LOCUS       DAJSBC010000001        87929 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSBC010000001 DAJSBC010000000
VERSION     DAJSBC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.001"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(552..2165)
                     /locus_tag="ONT65_000001"
     CDS             complement(552..2165)
                     /locus_tag="ONT65_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2327668.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="ONT65_000002"
     CDS             complement(2306..3214)
                     /locus_tag="ONT65_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2327669.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="ONT65_000003"
     CDS             3397..4521
                     /locus_tag="ONT65_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2327670.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4748..5665
                     /locus_tag="ONT65_000004"
     CDS             4748..5665
                     /locus_tag="ONT65_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2327671.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="ONT65_000005"
     CDS             5658..6491
                     /locus_tag="ONT65_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2327672.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="ONT65_000006"
     CDS             6574..7692
                     /locus_tag="ONT65_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2327673.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="ONT65_000007"
     CDS             7686..9584
                     /locus_tag="ONT65_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR2327674.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQISEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="ONT65_000008"
     CDS             9578..10801
                     /locus_tag="ONT65_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2327675.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="ONT65_000009"
     CDS             complement(10887..12821)
                     /locus_tag="ONT65_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2327676.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="ONT65_000010"
     CDS             13047..13703
                     /locus_tag="ONT65_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2327677.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="ONT65_000011"
     CDS             complement(13777..14130)
                     /locus_tag="ONT65_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2327678.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="ONT65_000012"
     CDS             14322..15251
                     /locus_tag="ONT65_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2327679.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="ONT65_000013"
     CDS             15262..16098
                     /locus_tag="ONT65_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR2327680.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="ONT65_000014"
     CDS             16346..17122
                     /locus_tag="ONT65_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2327681.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="ONT65_000015"
     CDS             17298..18041
                     /locus_tag="ONT65_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2327682.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="ONT65_000016"
     CDS             18034..19614
                     /locus_tag="ONT65_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR2327683.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="ONT65_000017"
     CDS             complement(19865..20344)
                     /locus_tag="ONT65_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2327684.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="ONT65_000018"
     CDS             complement(20360..21112)
                     /locus_tag="ONT65_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2327685.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="ONT65_000019"
     tRNA            21229..21299
                     /locus_tag="ONT65_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="ONT65_000020"
     CDS             21660..22385
                     /locus_tag="ONT65_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2327686.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="ONT65_000021"
     CDS             22640..22906
                     /locus_tag="ONT65_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR2327687.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="ONT65_000022"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="ONT65_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR2327688.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="ONT65_000023"
     CDS             23359..23664
                     /locus_tag="ONT65_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR2327689.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24233..25070
                     /locus_tag="ONT65_000024"
                     /pseudo
     CDS             24233..25070
                     /locus_tag="ONT65_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            25135..25569
                     /locus_tag="ONT65_000025"
     CDS             25135..25569
                     /locus_tag="ONT65_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2327690.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25637..26959)
                     /locus_tag="ONT65_000026"
     CDS             complement(25637..26959)
                     /locus_tag="ONT65_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR2327691.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27113..28753)
                     /locus_tag="ONT65_000027"
     CDS             complement(27113..28753)
                     /locus_tag="ONT65_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR2327692.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29083..29553
                     /locus_tag="ONT65_000028"
     CDS             29083..29553
                     /locus_tag="ONT65_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR2327693.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30182..31285
                     /locus_tag="ONT65_000029"
     CDS             30182..31285
                     /locus_tag="ONT65_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2327694.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31569..32249
                     /locus_tag="ONT65_000030"
     CDS             31569..32249
                     /locus_tag="ONT65_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2327695.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32339..33373
                     /locus_tag="ONT65_000031"
     CDS             32339..33373
                     /locus_tag="ONT65_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2327696.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33387..33722
                     /locus_tag="ONT65_000032"
     CDS             33387..33722
                     /locus_tag="ONT65_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327697.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33706..36186
                     /locus_tag="ONT65_000033"
     CDS             33706..36186
                     /locus_tag="ONT65_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327698.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36242..37801
                     /locus_tag="ONT65_000034"
     CDS             36242..37801
                     /locus_tag="ONT65_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2327699.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37798..39162
                     /locus_tag="ONT65_000035"
     CDS             37798..39162
                     /locus_tag="ONT65_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2327700.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39333..40403
                     /locus_tag="ONT65_000036"
     CDS             39333..40403
                     /locus_tag="ONT65_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2327701.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40418..41086
                     /locus_tag="ONT65_000037"
     CDS             40418..41086
                     /locus_tag="ONT65_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2327702.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41106..41684
                     /locus_tag="ONT65_000038"
     CDS             41106..41684
                     /locus_tag="ONT65_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2327703.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42087..42998
                     /locus_tag="ONT65_000039"
     CDS             42087..42998
                     /locus_tag="ONT65_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2327704.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43254..44171)
                     /locus_tag="ONT65_000040"
     CDS             complement(43254..44171)
                     /locus_tag="ONT65_000040"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR2327705.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44373..44810
                     /gene="zapA"
                     /locus_tag="ONT65_000041"
     CDS             44373..44810
                     /gene="zapA"
                     /locus_tag="ONT65_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR2327706.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44868..45398
                     /locus_tag="ONT65_000042"
     CDS             44868..45398
                     /locus_tag="ONT65_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR2327707.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            45530..47890
                     /locus_tag="ONT65_000043"
     CDS             45530..47890
                     /locus_tag="ONT65_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2327708.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47977..48291
                     /gene="trxA"
                     /locus_tag="ONT65_000044"
     CDS             47977..48291
                     /gene="trxA"
                     /locus_tag="ONT65_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR2327709.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48473..50251
                     /gene="uvrC"
                     /locus_tag="ONT65_000045"
     CDS             48473..50251
                     /gene="uvrC"
                     /locus_tag="ONT65_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR2327710.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50396..51295
                     /locus_tag="ONT65_000046"
     CDS             50396..51295
                     /locus_tag="ONT65_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2327711.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51288..52550
                     /locus_tag="ONT65_000047"
     CDS             51288..52550
                     /locus_tag="ONT65_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2327712.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(52644..53936)
                     /locus_tag="ONT65_000048"
     CDS             complement(52644..53936)
                     /locus_tag="ONT65_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2327713.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            54220..56118
                     /locus_tag="ONT65_000049"
     CDS             54220..56118
                     /locus_tag="ONT65_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR2327714.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(56417..57136)
                     /locus_tag="ONT65_000050"
     CDS             complement(56417..57136)
                     /locus_tag="ONT65_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR2327715.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            57473..57805
                     /locus_tag="ONT65_000051"
     CDS             57473..57805
                     /locus_tag="ONT65_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327716.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            57792..59795
                     /locus_tag="ONT65_000052"
     CDS             57792..59795
                     /locus_tag="ONT65_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR2327717.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            59814..60284
                     /locus_tag="ONT65_000053"
     CDS             59814..60284
                     /locus_tag="ONT65_000053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR2327718.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            60305..60889
                     /locus_tag="ONT65_000054"
     CDS             60305..60889
                     /locus_tag="ONT65_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR2327719.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            60904..61890
                     /locus_tag="ONT65_000055"
     CDS             60904..61890
                     /locus_tag="ONT65_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR2327720.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            61880..62191
                     /locus_tag="ONT65_000056"
     CDS             61880..62191
                     /locus_tag="ONT65_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR2327721.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            62213..63994
                     /locus_tag="ONT65_000057"
     CDS             62213..63994
                     /locus_tag="ONT65_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR2327722.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            63987..65363
                     /locus_tag="ONT65_000058"
     CDS             63987..65363
                     /locus_tag="ONT65_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR2327723.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            65370..66005
                     /locus_tag="ONT65_000059"
     CDS             65370..66005
                     /locus_tag="ONT65_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR2327724.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            66035..67390
                     /locus_tag="ONT65_000060"
     CDS             66035..67390
                     /locus_tag="ONT65_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR2327725.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(67582..68976)
                     /locus_tag="ONT65_000061"
     CDS             complement(67582..68976)
                     /locus_tag="ONT65_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2327726.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            69068..70039
                     /locus_tag="ONT65_000062"
     CDS             69068..70039
                     /locus_tag="ONT65_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR2327727.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            70036..70818
                     /locus_tag="ONT65_000063"
     CDS             70036..70818
                     /locus_tag="ONT65_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR2327728.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(70987..71358)
                     /locus_tag="ONT65_000064"
     CDS             complement(70987..71358)
                     /locus_tag="ONT65_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2327729.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            71492..71935
                     /locus_tag="ONT65_000065"
     CDS             71492..71935
                     /locus_tag="ONT65_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR2327730.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(71978..72787)
                     /gene="yidA"
                     /locus_tag="ONT65_000066"
     CDS             complement(71978..72787)
                     /gene="yidA"
                     /locus_tag="ONT65_000066"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR2327731.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(72806..74173)
                     /locus_tag="ONT65_000067"
     CDS             complement(72806..74173)
                     /locus_tag="ONT65_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2327732.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(74157..74987)
                     /locus_tag="ONT65_000068"
     CDS             complement(74157..74987)
                     /locus_tag="ONT65_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR2327733.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            75150..75587
                     /locus_tag="ONT65_000069"
     CDS             75150..75587
                     /locus_tag="ONT65_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR2327734.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            75663..76283
                     /locus_tag="ONT65_000070"
     CDS             75663..76283
                     /locus_tag="ONT65_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR2327735.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            76472..76978
                     /locus_tag="ONT65_000071"
     CDS             76472..76978
                     /locus_tag="ONT65_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR2327736.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            76995..77894
                     /locus_tag="ONT65_000072"
     CDS             76995..77894
                     /locus_tag="ONT65_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR2327737.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            77981..78865
                     /locus_tag="ONT65_000073"
     CDS             77981..78865
                     /locus_tag="ONT65_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2327738.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(79057..79128)
                     /locus_tag="ONT65_000074"
     tRNA            complement(79057..79128)
                     /locus_tag="ONT65_000074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79093..79095),aa:Arg,seq:ccg)
     gene            79188..80522
                     /gene="rpoN"
                     /locus_tag="ONT65_000075"
     CDS             79188..80522
                     /gene="rpoN"
                     /locus_tag="ONT65_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR2327739.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            80671..81762
                     /locus_tag="ONT65_000076"
     CDS             80671..81762
                     /locus_tag="ONT65_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR2327740.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            81803..82804
                     /gene="gap"
                     /locus_tag="ONT65_000077"
     CDS             81803..82804
                     /gene="gap"
                     /locus_tag="ONT65_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2327741.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            82914..84104
                     /locus_tag="ONT65_000078"
     CDS             82914..84104
                     /locus_tag="ONT65_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR2327742.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            84208..84963
                     /locus_tag="ONT65_000079"
     CDS             84208..84963
                     /locus_tag="ONT65_000079"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR2327743.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            85079..86377
                     /gene="eno"
                     /locus_tag="ONT65_000080"
     CDS             85079..86377
                     /gene="eno"
                     /locus_tag="ONT65_000080"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR2327744.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            86776..87915
                     /locus_tag="ONT65_000081"
     CDS             86776..87915
                     /locus_tag="ONT65_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR2327745.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        28902 a        15226 c        18141 g        25660 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ctgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg
    23701 attgtgacaa acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc
    23761 ccctcggcat tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc
    23821 taataatcag gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca
    23881 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt
    23941 ccttgtgact cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
    24001 cttgcatctg cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg
    24061 ctgttcttat tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa
    24121 tttccttaaa aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa
    24181 agaaaagagt aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg
    24241 tctgatttat tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg
    24301 tggaggcgtg ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat
    24361 ttctttttac tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt
    24421 atcaagcggg aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt
    24481 tggaggagta gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa
    24541 gcatgtgcag ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta
    24601 caccaagcat catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg
    24661 ggggggttct gggcttttta gctagttttt taggtattgg tggtggacca gtcaatgttt
    24721 ctttgttgat gctgatgttt gcattgccaa taaaagaagc aacactatat tctctatcta
    24781 cgattttctt ttctcaactg gcaaaattgg taacgattgc tttaacaagt agctttatgc
    24841 gctttgattt atctatgttg ttttatgtta tccccgctgc tgttattgga ggtttgtggg
    24901 gagcaagatt tagcagggtt ttgtctccaa aaaaagtgac ctttattttt caagtaattg
    24961 tcctcgtggt tcttttggtc aattgttaca atgcctttgt catcttacaa acattaggag
    25021 aatctagact aacgcaagtc aaaatcactg aaacatattg ttctaaatga aaaaaagagt
    25081 atactatgat aaaaaaattc attttaaaaa atcagaaatt ggctggtgat ttggatgtta
    25141 cgggaaatgc gtttagaaga tgcagaagca ctacgtgaaa tcaatgcgga tcaattaggg
    25201 tatgatatac cattaaccgt tacggtgaga caaatgaaga aactgctgaa ggagccagag
    25261 aaaaattttt tcttggttta tgaagatcct acttctaaaa aagcagctgg ctatgtacaa
    25321 gcggagtcct atgaatcgat ttttagcgat ccaatgttca atattatggc acttgctgtg
    25381 tctaaatatg ctgaaaaaag aggcatcgga aaggctttga tgactggcgt agaagaagaa
    25441 gcgaaaaaac gaggaataac gacaataagg cttaattctg cagaatacag agtagaagca
    25501 caccaatttt atgaacacat cggttatcat tccgataaaa tgcaaaaaag atttttgaaa
    25561 atcctgtaaa tctttatcaa aaaaataccc gatcaccaag ccgatacttg atgatcaggt
    25621 atttttgttt ttttgactaa gaaagtaatt gcatcaagaa accagagatg atcaaaacga
    25681 tggcaccacc aagtcgattg cccatttgag caaaagcaat aagttccatc cgattagctg
    25741 cagataagac ggctacattt cctgttccgc ccatcgagtt gttgcataag ccagcagtaa
    25801 tagttgattc aattggatat aaaccaaaca aacgaccgac aaatcctgaa acaactgaaa
    25861 tcgcgataac acttgtcaga cataagacaa caaattgcca tgtcaaagca gaagctaata
    25921 cattcagatt cagcaacgca ataccgatac cggcaagaac agctgctgtc aagtttttga
    25981 caatcaagtt attgaacatg atagctgcat cttcataata ctctggtaat aggttggcga
    26041 ttttacagat gacaacgata ataatcataa atgcgtaagc atggacttta ggaacaaaat
    26101 agttaccaat ttgtcctaag atgaagaaag tcaatgatac catcaaacca acacctaact
    26161 gtggaatgga aggtttcaca gttcgaggtt ttccatcgcc aagatcgcct ttttccatta
    26221 atttaccatt gccgttgaat tttggcattg attgtccagc acgagcaact aatgctgcac
    26281 cgatgatagc taagacattt gtcattgctg tcgcaggaat caggcgggaa atgatagaag
    26341 aagcatccgt acctaaaaca tttgcataaa tcgttgataa aggaacggct ccggctccaa
    26401 caccaccagc catcgcaggt aatgagatgt ataaaacgga atttgcaaaa ccatttccga
    26461 taagcatgcc cacgagtcct acggcaaaaa aggagacgac cattgaaata agtgcaacgg
    26521 gaataaaacg aacagaagct ttcatcaata gattacggtt cataccaagg atgctaccag
    26581 taatcaaggc agcaatataa aagtcccaaa agcccatatt gctgacaaag ttttctgtgg
    26641 atttcactac atattcaggt aagatcccaa atgtagcaat cgcagcagaa gcgaagatcg
    26701 caaaaacgga accaccaccc aaataacttt tgacgatagg gatttttgtc ccgataaaat
    26761 gaaataaatt accgaaaata accaaaactg cgattggtcc aatcatattc gtcggtaatt
    26821 tccctaaagc cattgcaatg atcaggacaa ggaccatgat caaataaagt ggcaaactta
    26881 cgccggtgat ttttgtggac caaaaacctt cttttttctt ttcctcaact gcagttgcag
    26941 ttgttgtttt ttgtgccatt tttacagatc ctttcttatt ctgtatcata tgaaaaaagc
    27001 caaagaaaag cgacaaaaga gcaggagttg gctggccgct gttttgccgc agtcgctttt
    27061 ttctttttag acgtcataca aagtttctgt atgttctttt actataacga tcttaatctt
    27121 ctaaagattt atattcagga acccatttag cagattcaac tgcttctttg atatccgtga
    27181 tttcttcacg attcaagttt tgtgcgacaa ctgattttgc gacagtttca gcaatcgttt
    27241 gagaaaattc agtgattttt gctactggag gcaagatagc tgctcctggt tttgttacat
    27301 caacgattcc gcctaatgct cgactagctt gagaaatgat ttcagcattt actcgtgtag
    27361 ctgttgaagc aatcaaacca agtcctaaac ctgggtacat caaagcatta tttgcttgac
    27421 cgatttggta agttacacca ttatattcaa catcagcagc agggatacct gttccaacta
    27481 atgcttttcc gtccgtccat tctattaaat cttttgcttt tgcttctgcc aattttgttg
    27541 gattagacaa aggcatgatg atcggacgtg gtgtatgagc agccatttct ttcacgattt
    27601 cttcagtgaa tgctccaggt tgggtagaag taccaatcat gattgtcgga tggatcgctt
    27661 ttacaactgc ttctaagttt gtcaattcat cagcattagt gaaatctgag cgtttgcgag
    27721 cgaatgggat ttgaccagga gtcaatcctt ctgtatcttc aaacaataaa ccttgtttat
    27781 ctacttgata gaaatgttgg cgtgcttctt cttcaggtac accttgacgg atcatctcat
    27841 ctaaaaggat gtttgcgata cccacaccag cagtaccagc accaaatgtc aaaattattt
    27901 gatctttcaa ttgttcaccg gatatattca atccgcctaa tacacctgct aggacaacaa
    27961 ttcctgtacc ttggatatcg tcgttgaaag tagtgatttg atcttcgtat ttttctaaga
    28021 tcgctgcagc atttcctcgt ccaaaatctt cccaatgcaa taacaattct gggaataatt
    28081 cggtagctga ttgaacgaat tgatcgatga attcatagta agtttcgcct tctacacgag
    28141 catgacgatt gcctaagtat aagggattat tcaataattc ttcattatta gtaccagcgt
    28201 cgatcgatac aggcaaaact tgtgctggat taataccagc agcagcagta tagaccatca
    28261 atttgccgat agcgatgtca acaccgttca caccccagtc gcccatacct aagattcctt
    28321 ctgcatcagt cacaacaatc aaacgaatgt ctcgtccatc tgccgcgttc ttcaaacttg
    28381 ctttgatgtt ttctggttcg tcgattgaaa gaaatgcagc atcttgcggt ttcaagaaaa
    28441 tttcgttgta ttgttcgatt gaatccgcaa cgacaggatc ataaacgaca ggcatgaatt
    28501 caactaaatg ttcgcccatc aatttataaa acaatgtccg attcgtattg aatagattca
    28561 ttaagaagat acgtttttcc aaatctgttt gtttgcttaa gtattgacca taagcttgaa
    28621 cagattgttg ttccagtgtt tggacagtac ttggaagcat accagtgatt ccatattttg
    28681 cccgttcttc ttttgtaaaa gctgttcctt tatttagaaa aggattattt aataagttta
    28741 accctgtaag catgaatgat tcctcctaag ttttaataca aacgaagtat agaagtcaga
    28801 tgatgatata gtcaaaaaga gcaggtatga taaattttat ttatatgtaa tatagccaaa
    28861 agaaatcaaa atagcagtat ttgttctttt tatatcgaca atccttgctt gaatactatg
    28921 cgtatctctt atatgctaga gaaagaaaag tagaagaata agggatagat ggaatggata
    28981 aataggattt ctgaggaaaa aagcacatga tagcgttgat aggagtcacc tatgtcaggt
    29041 gataaaaata cataagtaga aaattaagag gaggaagaga aaatgaaaga agttcaattg
    29101 attagaaaaa gcgaactatc agaaggcggt tgcaatgcat gtggcgtagt agaagcgaca
    29161 agctatacat taaaattaga ttccaatcaa gcaataatct ctgaactgac agtaggcggt
    29221 cttgtggaca gtctggcgct tgctgaaggt tttactggag aagatattta tgagatgttt
    29281 tcagaagtcc gtcagttaaa aaaaggagaa aaatgcatag aggttcatca tgagagcccg
    29341 aatgtgaggt tcaaacgtgg tgataatgag atgatcttca agaatcatgt gtctgatcac
    29401 actgaactat atgaaatcgt gaatcaaata ctgtctggga tttttgaatt aggaccttat
    29461 gagttcaagg aagaaaacgg taatccaaaa ttaaatgaag aatggcaaga aacgattgaa
    29521 acacaaagaa acaatccaca cttatttcaa tagtcttcca cagtatttcg taaaaatgtt
    29581 gatgaaaaag aaacaccgtg aatcagaggt cgtgaaaaac ctgccctgca tcaagtaata
    29641 agaacagcca aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgcag
    29701 atgcaagttt ttgaacaccg tttaaggaag agactgtgaa gtcaacgttc tgacctgagt
    29761 attagagaga caaatggcaa aatagttcct catattttgc cattttttga gctaataccg
    29821 agggtcagct gattgaacac cgttttatcg agaggttgtg aaaaagtcgc ttagtttcga
    29881 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttaata
    29941 ccgagaaaat ggcttttgaa caccgtttat tcaaaaaaag ggactttgac actacttttg
    30001 tcaaagtccc atcggttttt tatctatttt tattacaatc atttcttcca gagtagtgac
    30061 atctggaatg gctgaaatgt aaacagatta tttgcctgta tcaatgtcga agagtaatat
    30121 aaaaagtgta aacgctatca tattgttcat cattcaaatt ttatagagag aaggcattga
    30181 gatggaaaaa acatatcaag caggtacaaa tgaaggaata gtagatttta tcaatatgga
    30241 agatttagaa atcgcggctt cacaagtgat tccagctggt ggttatggtt acatcagcag
    30301 tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc atcgtttgat
    30361 tattcctcat gtattaagag atgtagaatt acccgataca actacacatt ttgacgaaga
    30421 aatgctgact gcaccaatca tcatggcacc agttgctgct catggattag ctcatgtgaa
    30481 ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata cagcaagttc
    30541 ttacgcttct tgtactttag aagagataag agaagcaggc ggggagaaag caccgcaatg
    30601 gtttcaattt tatatgagca aagataatgg aatcaatttg gatattcttg aagtggccaa
    30661 gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg gaaaccgtga
    30721 aacagaccgc agaaacgggt ttaccttccc gctgccaatg ccgatcgttc aggcttatca
    30781 atcaggagta ggccaaacga tggatgctgt atataaatct tccaaacaaa aacttagtcc
    30841 aaaagatgta gaatttatcg ctgctcattc tgatttgcca gtctacgtga aaggtgttca
    30901 atcagaagaa gatgtctatc gatcattaga atctggtgct ggtggaattt gggtatctaa
    30961 ccatggtggc cgtcaattag atggggggcc tgctgcgttt gattcgttgc aatatgtggc
    31021 agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac gtggacaaca
    31081 tgttttcaaa gcaatcgcat caggtgccga tcttgttgct attggcagac ccgttattta
    31141 tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct ttaaaacaga
    31201 attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca aaaaaataaa
    31261 attgcgtgag aatcgtttta tttaagaaat aaatgacgaa aaaaatagga gtgcggcaga
    31321 tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa tttgaaacga
    31381 aaaaataaaa ttgaaatatt ttttcatact taataaaaaa ggaagaatat tcatgtaaac
    31441 gattttattg tttttttctc ttttatacaa aaagggtcat aatttcaagt aagcggatga
    31501 attcatgagg attttttgca tactgtctct actatactta ggtcactaag aaaaaggagt
    31561 gatttattat gaaaaagaac atgattggga cattattact ttcaacactt cttctaggcg
    31621 gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat attaccttta
    31681 ctgaaccaac aaataccgtt gagcctctta accctactga tccttcaaaa ccagtagagc
    31741 cagcagatcc ggaaaatcca gcaactggcc agacaggttc tttaacattg gatgtagttc
    31801 ctgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat caagtagacg
    31861 cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta ggtgcagatg
    31921 gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac ggttcacaag
    31981 ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg tcagataacg
    32041 aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt gcagcgacac
    32101 caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt tatgatccag
    32161 caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct gatttgactt
    32221 ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa actgcggtga
    32281 aaatactata ggtgaaggat tttttaccgc agttgttgtt gaaaggatgt ggtggacaat
    32341 gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat ttttcatttt
    32401 tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac cggtatttcc
    32461 agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac cggaacaaaa
    32521 tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc
    32581 tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac
    32641 gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat
    32701 caatgtatca gcacatgcag aaacaactat acctatagaa gtgaaaatcc cagcaaaaag
    32761 ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag ctgaaacaga
    32821 aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt
    32881 acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag atttggacga
    32941 ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta ggattatcag
    33001 caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg aaaatacgtc
    33061 taatgaattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt tagctggtga
    33121 taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg acagtaatga
    33181 atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa
    33241 ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat tggttgctgc
    33301 agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagg
    33361 agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga gggattattg
    33421 caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa agttcaggta
    33481 cgattacatt ttatgatcta agtccagttc cgcatccaag tggttcagtc aatggcgcat
    33541 caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc gtattcccgg
    33601 agacgaatga agaaaagatc cgttttcttt atttgatcat tggtatagaa ttatggcttc
    33661 ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt gctacaaaat
    33721 aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag tatatttatc
    33781 ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta tgatccatac
    33841 acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt
    33901 gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt gaacgatggt
    33961 tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca tgtgaaacta
    34021 acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt ggcgatcatc
    34081 aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg gaacaacgcc
    34141 tacttgtata ttgccggtgc ccccatcaag gttactagcg gtagtttatt atcagcagga
    34201 agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt ggcagatatt
    34261 attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca atttgatgtt
    34321 acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag tccaaacaaa
    34381 attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt ttctgttgat
    34441 ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt agctccagct
    34501 gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc atatgcattt
    34561 tctgcaagtg cagagataaa tacgtggaat agttacatct caacaagtga ttcaacagga
    34621 aaatataata catttgctga taccacagaa tacgtggatg gaaataacca gaaccttcca
    34681 ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt tgatcagttg
    34741 ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt tacacaagga
    34801 gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc caatatcgtg
    34861 tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa ggtattggag
    34921 cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag tcagtgggga
    34981 aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac tgaactaaaa
    35041 aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc gacaggatgg
    35101 acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac agctacttct
    35161 acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt ggacgatgta
    35221 cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc tggaatcacc
    35281 actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag tcagctaaaa
    35341 aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg agatacgatt
    35401 caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga caaaaaaatc
    35461 acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt aaatggtaca
    35521 actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct attgagcaaa
    35581 ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga tacttctgta
    35641 caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac attagcagat
    35701 ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct tgtccccaaa
    35761 caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga tctccccaaa
    35821 ccgttgaaga caagttatta tgaagcaaaa ggcgattttt cttttgatgt gcgggatact
    35881 cggcttccaa gcacgtctcc ttggcaactg acagggacat tgacttcttt atttaagaat
    35941 gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg atcaaaacaa
    36001 ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc taaaggagag
    36061 gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag cagtacgaat
    36121 gctcaacctg gcgcaacgta tcaaggcatg gtcacatggg aactgacagc tgggccaacc
    36181 agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct ctttttcaag
    36241 gatgagaagg aaacttctgc tgttaaaatc agaaatgctt ggaggagtgg acttggaact
    36301 tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac ttttattaga
    36361 ggaaaaagga gagatttccg ttaataacgt acttacccgc ttatcagttg atcgggctac
    36421 cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac aattggaaat
    36481 tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag cagtcgatgt
    36541 ttattatcat tgctacttgc gctattctac aaaatatcag atacttcttt atctgttgga
    36601 aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt cggctacttt
    36661 gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga aaataaaaag
    36721 cgggcggctg accggtccag aaagtcaaat ttgttatttt tatttccaat tattttggtt
    36781 tggacgtcct tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa acactttttt
    36841 agatctgctg caaaaagagt ttagttttac tattacagaa gaaggacgat tgaaatttga
    36901 aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca aaccagatta
    36961 tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc tccatacatt
    37021 tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa cgttggtttt
    37081 ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg tccaggagtt
    37141 tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa cgtttaaaca
    37201 atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc aaaagattgc
    37261 gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga tgaatatttt
    37321 tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat tcctggtttt
    37381 atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag tagacgagga
    37441 aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttgcggacgc tgatcaagca
    37501 gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa tggcggatat
    37561 cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt tagtcgaata
    37621 cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt acccgtcaca
    37681 gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt gcccctattt
    37741 gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga ataaaacatg
    37801 aaaatcgtga tcattggtgc ttcccatgca ggcatcacag ctgccctaaa tctaagaaaa
    37861 cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg actaggctat
    37921 gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc tgaagttgcc
    37981 cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg gagagtaact
    38041 gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa aaacgagacc
    38101 ataacctatg ataaattaat cgtagcaaca ggttcctctc ctgtcacctt gtataaacaa
    38161 attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca agtacttgcc
    38221 gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttatattgg attggaatta
    38281 gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat gccgaatgtt
    38341 ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct gcaaactaag
    38401 caaatcaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga agagaaagta
    38461 gtatccgtac aacttctatc tcaagcaata aaggcagata tggtcatttt ttctgcccaa
    38521 actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga tgagactgtg
    38581 atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg agatatcgtc
    38641 ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac tcgagctgta
    38701 catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta cgacctcaga
    38761 caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg gctgacagaa
    38821 gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt tgatctattt
    38881 ccacaatacg atgaagaaaa caaaacagta aatgcaaagc tgatctacca tccggataca
    38941 ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga tttgaacctg
    39001 cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga ggattttgga
    39061 tttctgtctg aacacacctc tcgttttcac tacctcaatg agttagcttt taaagtacta
    39121 gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc acggtctttt
    39181 attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat ataatgaaat
    39241 agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat aaaattgttg
    39301 tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat gaaattatgc
    39361 attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc tacctggtat
    39421 tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca gtcgacaagt
    39481 gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg atgtccatga
    39541 ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt gcccagacac
    39601 ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt agcttcttcg
    39661 gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct tttgataacg
    39721 atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc gtaggagata
    39781 cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg gatcatgcca
    39841 aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagag atccgcttcg
    39901 aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga ggtaaaatca
    39961 gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat gaattacctg
    40021 gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc gttattgcgg
    40081 ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat atctttggtg
    40141 taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg gcacaaacat
    40201 tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga acatcccttg
    40261 ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc attagcttgt
    40321 tgatgctggt agtagctgtg ttaggcgcac tattttctgt tcggacaatc gtaaaaatcg
    40381 atccgttgaa ggcaattgga taagaaggag gaagagcatg aaagcaatag aatttaaaga
    40441 agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag aaacaaccat
    40501 gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag gtaaaagtac
    40561 ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat ttatcaatga
    40621 tcaagaattc agtaatcaaa aagaaagtga acgcgttaaa ctacgtttta aagaaattgg
    40681 ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc agttattatt
    40741 agtagataag atcaaacatg aacatcgtca aaaagaagca aaagaattat ttgagcagct
    40801 tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag aacggcaaag
    40861 ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag atgaaccaac
    40921 tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa aagaatcaaa
    40981 agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg acaaatgcga
    41041 tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa aaagcaggtg
    41101 gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa gaagcgactg
    41161 acttgtttat gtccaaggga tatctcggca cttcaacaag agagatcgca caaaaagttg
    41221 ggatcaccca acccaatctc tatcattatt tcggtgataa agaaaaacta tatacggcag
    41281 ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt caaacaagcg
    41341 aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag acacatctgg
    41401 tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa acaagagatc
    41461 atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc ttttcagcaa
    41521 accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat tttttcttag
    41581 tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta aatgtggcgc
    41641 aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt tatataggga
    41701 gaaaaatagt taggtgaaga agcaagatat gacgaagatc gttatatctt gtttttttta
    41761 tataggtagt ttttcataaa tacaaatttg ttattctctc atgattatca gggtatattt
    41821 ttatttatta tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt
    41881 ttgtagaatg aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag
    41941 atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt
    42001 tatatctttt acagcccctt ttttgcgtac ttaagctgat tttaagtttt gacccttagt
    42061 ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa
    42121 tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac
    42181 aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag
    42241 aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc
    42301 taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa
    42361 aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg
    42421 gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg
    42481 tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag
    42541 aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg
    42601 gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg
    42661 actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat
    42721 atgcgaatga agcaactgga atcacgacat attattacta tggacaagag gaaacattag
    42781 cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg
    42841 ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata
    42901 tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg
    42961 caagtttgac acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa
    43021 aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttgaac
    43081 accgtttaag gaagaggttg tgagcctgcc gtttaactcc gcgtcacaag gagcaatttc
    43141 aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcatgag
    43201 aacaccgttt attcggaaca agggactgtg acatgacttt tgtcacagtt ccattatttt
    43261 tttagcagct tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata tttttcaagc
    43321 ataggcatgc cgcctttctc caatagtttt gccgctactt ggtcagattt cgttccagct
    43381 cctgatggaa gggtgattcc agcttcagct gatgctttgt gtaactcttc caaaaagctg
    43441 gcacgactga tgatagaagc agcagcaaca gctaaatgat attgttctcc tttagtaacg
    43501 aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg catatttttt aaaatttgct
    43561 tccggcgtaa actgatcgat caaaatcgct tctggttttt ctggctcgat tttttttagc
    43621 agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt gtattttggt
    43681 tggatctcat tgtatttttc tggagataag acaagcagtt tataggggat ggtttttttt
    43741 aagatgtcag caagctggat gatttggcta tcttttaact cttttgaatc tctaacacca
    43801 agtttacgta attctgcaag ctgatcacgt ttcacataag cagcacatac tgttagcgga
    43861 ccaaaatagc tgccatttcc gacctcatca ctacctaaaa cggataattg actgatattt
    43921 tttggcaaag aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc ctccccccat
    43981 ttatcagctt ctattgctgc attcgtacct tgaaacatca cttttccgga tgtataagcg
    44041 gtaatggtcg tcccgtcttt ttttgcagca aagctagtat aaggaatctg tttatttaac
    44101 aaataagagc gatagtatgc tttcatttga ctaagttggt cgtttgttgc tttgataatg
    44161 acgttgctca taaaattctc ctcgttttta tacataaaac tataccgcaa ctcacgactt
    44221 ttcgttttct aagagattgc gagacatagc atttttttaa agtttatcat ataatagtta
    44281 agtatgtgaa aaatatttgt ggagaacggt gaaagaacca acttcatggt acactaaata
    44341 aaactttcat caaataggag gaaatgattc gtatggcgca cgaaaaaaca cgctacaaag
    44401 cagtgattgc caaccaaaca tatactatta tcggacggga aaccaagcat catatggata
    44461 tcgtaacgaa gctgatcaat gaacagttag cagaattgaa acaattatca ccacaaatgg
    44521 ataatgaaca agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa ttgaagaaac
    44581 aagaaagaat cttggagctg gaagaagaaa cagcagaatt aaagaaaaaa atgatcaaat
    44641 ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa gctcgggaag
    44701 tattgaaaga aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa gcacaacaga
    44761 ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa gatcctttta
    44821 tcaaaataag aaaaatttaa aaaagttaga agagaaagga acgtagcatg ctgagtttgc
    44881 tgattttatt tttattgcta attgcgtttt tttctggagc acgtcgcggg tttgctttgc
    44941 aaatcatatt cgcgattgga tatgtattgt catttattgc tgcccagcat ttttacaaac
    45001 cattagcaag tcatttggaa ttatacattc catacccagc tgtcacccca acatcaaagt
    45061 tagcattttt tgatcaggta tttgcttttc acctagatga agctttttat gcaggaactg
    45121 cttttctgat tattttgcta attggctggc tgcttacccg atttgtgggg gtttttgtcc
    45181 atggattgac gtatgtgccg atcttgagac aggtagactg gattgcagga ggcatcctgt
    45241 cactaatcat ggcttacgtt actatatttt tgattttgca gttacttgcc tttgttccat
    45301 tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc tcgttttatc gttgagagaa
    45361 cgccatttct taccaacaag atgtatgagc tttggtaacg aatattttga attaaggaaa
    45421 atagtgctgg aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag
    45481 cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg
    45541 aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac
    45601 tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac
    45661 aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc
    45721 tatacctaaa cttgaaaata tccgtcccca tatgaaacgg atcgagatcg gagcagactt
    45781 gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg
    45841 tttcattgat gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga
    45901 tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg
    45961 acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag
    46021 tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt
    46081 aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata
    46141 ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat
    46201 tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg
    46261 aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga
    46321 aatcatccac aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt
    46381 tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa
    46441 acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg
    46501 aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt
    46561 gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga
    46621 agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat
    46681 tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa
    46741 agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga
    46801 aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat
    46861 ggcaacaacg cattatcctg aattgaaagt ttatggatac aatcgggcaa atacgattaa
    46921 tgccagcatg gagttcgatg tggatacact aagtccaacg tatcgtttgc tgattggcgt
    46981 tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat
    47041 tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga
    47101 tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc
    47161 tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg
    47221 tgaaaaagaa atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga
    47281 aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa
    47341 tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga
    47401 aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg
    47461 agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa
    47521 tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc
    47581 tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag
    47641 tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga
    47701 ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca
    47761 cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag
    47821 cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa
    47881 atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta
    47941 tactaatcat agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag
    48001 acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt
    48061 ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa
    48121 gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt
    48181 atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt
    48241 ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca
    48301 gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga
    48361 gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt
    48421 ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga
    48481 acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga
    48541 taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc
    48601 atattttact ggcagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt
    48661 tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa
    48721 gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa
    48781 gataacgaat gaaaaatatc cccgtttggt gattactcgt aaagtactaa aagacaaagc
    48841 actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga
    48901 tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta
    48961 tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa
    49021 aatcgttgat gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg agtcagagat
    49081 ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg
    49141 agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt
    49201 actggatcga gatgtatttg gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt
    49261 tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga
    49321 agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat
    49381 tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc
    49441 tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa
    49501 gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac
    49561 gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga
    49621 gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat
    49681 tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc
    49741 ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcacgag tgattcgcga
    49801 agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc
    49861 aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa
    49921 cgataaacat aaaacaaatg agttgctatt cggtatagat ttggctgtag taccgcttga
    49981 gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc
    50041 aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat
    50101 tgaagggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa
    50161 tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca
    50221 aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag
    50281 tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat
    50341 cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt
    50401 agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt
    50461 ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac
    50521 gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg
    50581 ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg aacggggact
    50641 ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc
    50701 aagaaaagag attgaaccaa aaatcgattt ttggatggaa aaatttcagg taaaagggaa
    50761 aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc
    50821 cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc
    50881 ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt
    50941 cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt
    51001 gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg
    51061 tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt
    51121 gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc
    51181 tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca
    51241 accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt
    51301 ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga
    51361 tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa
    51421 atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga
    51481 gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa
    51541 aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga
    51601 taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac
    51661 aacaactgac tggcttgcaa tcgatgcttc gagcaagtca attaggcatt tcaccagaag
    51721 aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa
    51781 aaggaaaaat gacagttgga gaagataact cgacagtcca atatgtcgta agttatgtag
    51841 caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg
    51901 cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc
    51961 atttttacgg aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg
    52021 gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat
    52081 tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct cttctattca
    52141 tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca
    52201 aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggatata
    52261 tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt
    52321 atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta
    52381 tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct
    52441 tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt
    52501 cattgaaaca atcgatttca attatgagaa atgaacgaaa aaaagcataa aatatagaaa
    52561 aatggctctt tgtcaaatga tgttggtaaa gagaaatctt agaataattg gagatcaagt
    52621 ggatcagttc ctcttgatct tttttagttg aaaatcaatg cgttgccatg acgaatcatg
    52681 atgcgcaatt tgaaattata aaagctacga aagccatatg catttcgttt taacactttg
    52741 atatggttgt tcagacattc aagtgggcca tttgaataac tttgttgtaa agcatttttg
    52801 atttgtggca gaaatttctg gaacgttctc aaggtcgtct gatagccttc gggaagcatg
    52861 atattattgg taagtagttc ttcaaacgat acgtagtctc gttcctttac agcgtaaaga
    52921 aagtcttgat ataactgata gcctacacgc aaagcagggc tccctttcag taagcgatcc
    52981 acaatatcgg tctctgttaa atgagctttg aagccaggtc gccaaaggcg tcgagtataa
    53041 tcaagcttgc gttcatcttt ttgaagaagt ttccagtatc tttttaattg cttacctaat
    53101 tgatagtgtt tttgttcttt gctttttaat agttggttcg tttctttgat tcgatgattg
    53161 cgaaaggtac gaccaatatg ttggacgatg tgaaaccggt caatgatcag ctgtgctttt
    53221 ggaaacagct ttttaacgag cgatacataa ggagcgtaca tatcaatcac gatccattcg
    53281 accgcttcac gaatttctcg tgaaaaatga ctaaaataac gttttaaaaa aggcagttgt
    53341 cggttctcga caatgtccaa caagcgctgt gtttgtccat ccatcatgat gaaactcata
    53401 gcacctgaaa caccccgaac tgatttgaat tcatcaaaac aaagtacctt aggtaacggt
    53461 gtccgaaaag gattcaacgg acgataaaat cgcttcataa tacggtaaac agaagagacc
    53521 gatagatttt tctgagcggc gatctctgtc attgaggtat tttttttcag acgagtcacg
    53581 acagaccagc ggactttctc actgatgaaa cagtgttttt tcgcaacaga agtatctgcg
    53641 acaaaggtcc gctgacaagc tttgcatttg aagcgttgct ttttcaattc gaggtaggta
    53701 cggcattcag aaacatcgtt cattaacaac cggacggttg ttgtacccca acgcacaata
    53761 tctgaagcaa tctggtgttt acagtggatg cattcttggg ctgtataaat caagtttcca
    53821 cgataaatat gacagatttg ccctttgtat ttttcttttg tgagacaatt ttcgttaaaa
    53881 gtaactgata agtctagaat atctaatgtt tctttgataa ggtgagtgta agacatcttg
    53941 attcctcctt ttgggttggt tcgccaaatt aattctaaag gatttcaagg tgtcttttct
    54001 atatgttttg gaaaataaaa aagatgctgg tgtcatccac caacatcaaa aagtatagag
    54061 ctagaaaaat atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac
    54121 ctatttctat ttttttatta tattggatat atatattcat tgaaaataga gcataacaac
    54181 tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta
    54241 ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca
    54301 attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct
    54361 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
    54421 ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca
    54481 gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt
    54541 ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga
    54601 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac
    54661 gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa
    54721 aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc
    54781 gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat
    54841 ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc
    54901 ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt
    54961 gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc
    55021 tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa
    55081 gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag
    55141 ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac
    55201 tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt
    55261 caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat
    55321 agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc
    55381 gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct
    55441 gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
    55501 ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa
    55561 gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc
    55621 aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa
    55681 aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca
    55741 gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta
    55801 aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta
    55861 tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac
    55921 ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa
    55981 tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg
    56041 aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat
    56101 tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac
    56161 tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta
    56221 gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc
    56281 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca
    56341 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    56401 ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg
    56461 aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat
    56521 ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag
    56581 gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa
    56641 ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag
    56701 tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg
    56761 ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc
    56821 tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt
    56881 ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg
    56941 caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg
    57001 gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat
    57061 agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc
    57121 cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa
    57181 aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    57241 taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    57301 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    57361 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    57421 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    57481 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg
    57541 aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat
    57601 ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa
    57661 gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    57721 tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt
    57781 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    57841 atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt
    57901 atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg
    57961 ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc
    58021 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    58081 tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag
    58141 aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag
    58201 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    58261 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    58321 aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    58381 tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga
    58441 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
    58501 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
    58561 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    58621 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
    58681 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
    58741 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    58801 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
    58861 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
    58921 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
    58981 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
    59041 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
    59101 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
    59161 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
    59221 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
    59281 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
    59341 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
    59401 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
    59461 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
    59521 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
    59581 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    59641 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
    59701 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
    59761 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
    59821 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
    59881 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    59941 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
    60001 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
    60061 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
    60121 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
    60181 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
    60241 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
    60301 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
    60361 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    60421 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
    60481 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
    60541 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
    60601 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
    60661 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
    60721 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    60781 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    60841 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    60901 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    60961 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    61021 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    61081 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    61141 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
    61201 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    61261 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
    61321 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    61381 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    61441 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    61501 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    61561 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    61621 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
    61681 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    61741 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    61801 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
    61861 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    61921 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    61981 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    62041 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    62101 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    62161 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    62221 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    62281 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
    62341 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    62401 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    62461 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    62521 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    62581 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    62641 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    62701 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    62761 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    62821 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    62881 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    62941 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    63001 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    63061 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    63121 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    63181 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    63241 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    63301 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    63361 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    63421 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    63481 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    63541 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    63601 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    63661 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    63721 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    63781 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    63841 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    63901 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    63961 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    64021 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    64081 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    64141 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    64201 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    64261 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    64321 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    64381 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    64441 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    64501 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    64561 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    64621 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    64681 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    64741 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    64801 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    64861 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    64921 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    64981 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    65041 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    65101 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    65161 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    65221 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    65281 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    65341 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    65401 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    65461 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    65521 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    65581 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    65641 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    65701 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    65761 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    65821 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    65881 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    65941 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    66001 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    66061 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    66121 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    66181 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    66241 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    66301 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    66361 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    66421 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    66481 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    66541 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    66601 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    66661 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    66721 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    66781 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    66841 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    66901 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    66961 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    67021 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    67081 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    67141 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    67201 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    67261 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    67321 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    67381 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    67441 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    67501 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    67561 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    67621 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    67681 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    67741 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    67801 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    67861 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    67921 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    67981 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    68041 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    68101 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    68161 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    68221 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    68281 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    68341 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    68401 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    68461 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    68521 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    68581 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    68641 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    68701 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    68761 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    68821 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    68881 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    68941 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    69001 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    69061 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    69121 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    69181 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    69241 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    69301 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    69361 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    69421 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    69481 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    69541 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    69601 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    69661 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    69721 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    69781 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    69841 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    69901 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    69961 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    70021 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    70081 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    70141 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    70201 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    70261 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    70321 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    70381 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    70441 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    70501 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    70561 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    70621 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    70681 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    70741 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    70801 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    70861 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    70921 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    70981 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    71041 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg
    71101 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    71161 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    71221 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    71281 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    71341 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    71401 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    71461 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    71521 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    71581 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    71641 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    71701 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    71761 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    71821 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    71881 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    71941 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    72001 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    72061 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    72121 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    72181 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    72241 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    72301 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    72361 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    72421 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    72481 cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    72541 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    72601 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    72661 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    72721 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    72781 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    72841 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    72901 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    72961 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    73021 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    73081 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    73141 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    73201 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    73261 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    73321 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    73381 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    73441 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    73501 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    73561 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    73621 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    73681 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    73741 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    73801 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    73861 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    73921 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    73981 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    74041 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    74101 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    74161 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    74221 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    74281 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    74341 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    74401 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    74461 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    74521 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    74581 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    74641 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    74701 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    74761 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    74821 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    74881 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    74941 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    75001 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    75061 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    75121 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    75181 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    75241 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    75301 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    75361 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    75421 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    75481 gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac
    75541 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    75601 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    75661 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    75721 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    75781 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    75841 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    75901 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    75961 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    76021 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    76081 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    76141 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    76201 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    76261 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    76321 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    76381 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    76441 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    76501 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    76561 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    76621 tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc
    76681 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    76741 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    76801 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    76861 ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa
    76921 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    76981 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    77041 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    77101 agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt
    77161 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    77221 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    77281 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    77341 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    77401 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    77461 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    77521 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    77581 cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac
    77641 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    77701 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    77761 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    77821 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    77881 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    77941 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    78001 tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    78061 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    78121 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    78181 tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa
    78241 aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    78301 gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt
    78361 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    78421 gcacgagatt ggcaattaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    78481 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc
    78541 cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg
    78601 accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat
    78661 tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta
    78721 tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg
    78781 catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact attatggcag
    78841 aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta
    78901 ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta
    78961 caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag
    79021 caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc
    79081 cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat
    79141 aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc
    79201 aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac
    79261 agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag
    79321 caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag
    79381 cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa
    79441 aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt
    79501 tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta
    79561 gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga
    79621 ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca
    79681 tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat
    79741 gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc
    79801 tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga
    79861 ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg
    79921 ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga
    79981 gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac
    80041 ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc
    80101 gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa
    80161 tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca
    80221 gtcgtgcagt caacgggaaa tatttggaaa cagatttcgg tgtatttgaa ttaaaaaaat
    80281 ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata
    80341 cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtcgg
    80401 accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag
    80461 caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact
    80521 aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg
    80581 agagcgaaac tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat
    80641 tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta
    80701 aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga atctgttgca
    80761 ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg
    80821 cagccgattg ggcgtcgaag tttatcggaa agtatgggga tcacagaacg agtcttacga
    80881 acagaaacag accttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg
    80941 ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta
    81001 ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt
    81061 gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc
    81121 gaagccttag accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact
    81181 actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg
    81241 tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt
    81301 gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac tttatgttcc ggaacaatta
    81361 agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg
    81421 attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt
    81481 cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct
    81541 tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata
    81601 gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa
    81661 gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat
    81721 gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat
    81781 tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat
    81841 tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa
    81901 cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg
    81961 tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa
    82021 agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt
    82081 tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg
    82141 tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa
    82201 cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac
    82261 taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt
    82321 aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa
    82381 aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc
    82441 tgctaaagct atcggtttag taattcccga attgaacggt aaattagacg gagctgctca
    82501 acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt
    82561 aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa
    82621 cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc
    82681 tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta
    82741 cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt
    82801 ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc
    82861 aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta
    82921 agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca
    82981 acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa
    83041 caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg
    83101 taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg
    83161 aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag
    83221 caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata
    83281 ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag
    83341 gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag
    83401 gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt
    83461 tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa
    83521 aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga
    83581 tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc
    83641 tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac
    83701 ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag
    83761 taatcgaagg agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa
    83821 aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg
    83881 tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact
    83941 tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag
    84001 gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg
    84061 aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc
    84121 aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc tagccatttt
    84181 tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa
    84241 atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct
    84301 tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg
    84361 acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca
    84421 ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg
    84481 atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa
    84541 aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg cgaatcttta
    84601 gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta
    84661 gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc aatctgggca
    84721 atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt
    84781 caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc
    84841 ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct
    84901 ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa
    84961 taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact
    85021 aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat
    85081 gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat
    85141 cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc
    85201 ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg
    85261 taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg
    85321 ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc
    85381 taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc
    85441 agctgctgac tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt
    85501 attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga
    85561 cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat
    85621 gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc
    85681 tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt
    85741 aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat
    85801 ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgactc
    85861 aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa
    85921 atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg gattcaagaa
    85981 attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa
    86041 cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt
    86101 gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg
    86161 ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat
    86221 cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat
    86281 tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa
    86341 aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg
    86401 atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt
    86461 gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat gatgtttaca
    86521 aagaatggta caagaaaaga tcatccttga aaaaggaaag ctaacaataa ctagcatcat
    86581 ataagagctt ttaaggatag aacagtagaa taggaatcta tgggcaagtc ctctcgtata
    86641 aataaaatag gagggaagta gcagcatatt tttcataaag tggctctctt tcttcatttt
    86701 tactttggtt gctcctattt gaaaattcga gcacactcaa atacaataaa aagaaatggc
    86761 aagggagagg attagatgaa aaaagaagaa aaaatcgcga ttttacaaga gattatccga
    86821 attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct atttgaataa gcttttggca
    86881 aagcatgaca taacaggaga gattgtttct tacagagatg gtagagataa tttgattgct
    86941 cgttaccaaa agggccaatc aggaaaagtg ttaggcctat ctggacatat ggatgtcgtg
    87001 gcggctggtg atgaatcgtc ttggacctat gcaccctttg cagcagaaat ccatgggaac
    87061 cgtttgtacg gacgtggtgc tacagatatg aagagtggct tggcagcaat ggtgattgcc
    87121 atgatcgaac tgaaagagtc aggaaaacca tttaacggaa cagtcaaatt attagccact
    87181 gtcggagaag aagtgggtga actaggtggg gaacagctca cgaaagcagg ctatgtagat
    87241 gacttagatg ccttgatcat cggagagcca accaattaca gtttgatgta tacgcacatg
    87301 ggatcaatca actatacagt cacctcgcac ggaaaagaag cgcatagctc tatgccagac
    87361 caaggttata atgcaatcaa tcacctaaat gaattcatta cgaaagcaaa tgctgaaatg
    87421 aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa aaacaatcca taatgttaca
    87481 ttgatcagtg gcgggaatca agtcaacagc atcccaagcc atgcgcagct ccaagggaac
    87541 attcgatcga ttccagaata cccaaatgat aaaatcattg cattgctgca atcaatcgtc
    87601 aatgaattga atcaagagac agactatcat ttggaattga tgatcgatta caacaaaata
    87661 cctgtcaaag ctgatccaga ttctcctttg attcattgca ttcagcaaca attcagccag
    87721 ccattacctt tagttggagc ggcagcaaca acggatgcag ccgaatttac gaaagctaac
    87781 cattccttcg actttgtcgt ctttgggcct ggtgtcgtta ctttgcctca tcaagtagat
    87841 gagtatgttg aaatcgataa ttatttggat atgatcgaaa aatatcaagg aattatttta
    87901 tcctacttag cgtaagattt ttttaataa
//
LOCUS       DAJSBC010000002        87415 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSBC010000002 DAJSBC010000000
VERSION     DAJSBC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.002"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            164..1045
                     /locus_tag="ONT65_000082"
     CDS             164..1045
                     /locus_tag="ONT65_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2327746.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1058..2668
                     /locus_tag="ONT65_000083"
     CDS             1058..2668
                     /locus_tag="ONT65_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR2327747.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2745..3404)
                     /locus_tag="ONT65_000084"
     CDS             complement(2745..3404)
                     /locus_tag="ONT65_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2327748.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3687..4352)
                     /locus_tag="ONT65_000085"
     CDS             complement(3687..4352)
                     /locus_tag="ONT65_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR2327749.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4499..5104)
                     /gene="dhaL"
                     /locus_tag="ONT65_000086"
     CDS             complement(4499..5104)
                     /gene="dhaL"
                     /locus_tag="ONT65_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR2327750.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5132..6142)
                     /gene="dhaK"
                     /locus_tag="ONT65_000087"
     CDS             complement(5132..6142)
                     /gene="dhaK"
                     /locus_tag="ONT65_000087"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR2327751.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6117..6563)
                     /gene="dhaM"
                     /locus_tag="ONT65_000088"
     CDS             complement(6117..6563)
                     /gene="dhaM"
                     /locus_tag="ONT65_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR2327752.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6666..6986
                     /locus_tag="ONT65_000089"
     CDS             6666..6986
                     /locus_tag="ONT65_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR2327753.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7098..7967)
                     /locus_tag="ONT65_000090"
     CDS             complement(7098..7967)
                     /locus_tag="ONT65_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2327754.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8063..9366)
                     /locus_tag="ONT65_000091"
                     /pseudo
     CDS             complement(8063..9366)
                     /locus_tag="ONT65_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9363..9872)
                     /locus_tag="ONT65_000092"
     CDS             complement(9363..9872)
                     /locus_tag="ONT65_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327755.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10047..12182)
                     /locus_tag="ONT65_000093"
     CDS             complement(10047..12182)
                     /locus_tag="ONT65_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2327756.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12265..13920)
                     /locus_tag="ONT65_000094"
     CDS             complement(12265..13920)
                     /locus_tag="ONT65_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2327757.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13922..14236)
                     /locus_tag="ONT65_000095"
     CDS             complement(13922..14236)
                     /locus_tag="ONT65_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2327758.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14406..14666
                     /locus_tag="ONT65_000096"
     CDS             14406..14666
                     /locus_tag="ONT65_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR2327759.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14680..14994
                     /locus_tag="ONT65_000097"
     CDS             14680..14994
                     /locus_tag="ONT65_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR2327760.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15724..16278)
                     /locus_tag="ONT65_000098"
     CDS             complement(15724..16278)
                     /locus_tag="ONT65_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR2327761.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIRLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16404..18332)
                     /locus_tag="ONT65_000099"
     CDS             complement(16404..18332)
                     /locus_tag="ONT65_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2327762.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18638..19354)
                     /locus_tag="ONT65_000100"
     CDS             complement(18638..19354)
                     /locus_tag="ONT65_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2327763.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19355..21178)
                     /gene="glpO"
                     /locus_tag="ONT65_000101"
     CDS             complement(19355..21178)
                     /gene="glpO"
                     /locus_tag="ONT65_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR2327764.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21192..22688)
                     /gene="glpK"
                     /locus_tag="ONT65_000102"
     CDS             complement(21192..22688)
                     /gene="glpK"
                     /locus_tag="ONT65_000102"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR2327765.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22824..24284)
                     /locus_tag="ONT65_000103"
     CDS             complement(22824..24284)
                     /locus_tag="ONT65_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2327766.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24274..25569)
                     /locus_tag="ONT65_000104"
     CDS             complement(24274..25569)
                     /locus_tag="ONT65_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2327767.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25901..26578)
                     /locus_tag="ONT65_000105"
     CDS             complement(25901..26578)
                     /locus_tag="ONT65_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR2327768.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26608..27699)
                     /locus_tag="ONT65_000106"
     CDS             complement(26608..27699)
                     /locus_tag="ONT65_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR2327769.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27800..28234)
                     /locus_tag="ONT65_000107"
     CDS             complement(27800..28234)
                     /locus_tag="ONT65_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2327770.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28818..30275)
                     /gene="ascB"
                     /locus_tag="ONT65_000108"
     CDS             complement(28818..30275)
                     /gene="ascB"
                     /locus_tag="ONT65_000108"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2327771.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30272..32167)
                     /locus_tag="ONT65_000109"
     CDS             complement(30272..32167)
                     /locus_tag="ONT65_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2327772.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32225..34000)
                     /locus_tag="ONT65_000110"
     CDS             complement(32225..34000)
                     /locus_tag="ONT65_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2327773.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34050..35546)
                     /locus_tag="ONT65_000111"
     CDS             complement(34050..35546)
                     /locus_tag="ONT65_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2327774.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35740..37362)
                     /locus_tag="ONT65_000112"
     CDS             complement(35740..37362)
                     /locus_tag="ONT65_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2327775.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37811..39124)
                     /locus_tag="ONT65_000113"
     CDS             complement(37811..39124)
                     /locus_tag="ONT65_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2327776.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39369..39593)
                     /locus_tag="ONT65_000114"
     CDS             complement(39369..39593)
                     /locus_tag="ONT65_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327777.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39613..40035)
                     /locus_tag="ONT65_000115"
     CDS             complement(39613..40035)
                     /locus_tag="ONT65_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR2327778.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40179..40889)
                     /locus_tag="ONT65_000116"
     CDS             complement(40179..40889)
                     /locus_tag="ONT65_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327779.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40907..41404)
                     /locus_tag="ONT65_000117"
     CDS             complement(40907..41404)
                     /locus_tag="ONT65_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2327780.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41441..41893)
                     /locus_tag="ONT65_000118"
     CDS             complement(41441..41893)
                     /locus_tag="ONT65_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2327781.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(42028..42633)
                     /locus_tag="ONT65_000119"
     CDS             complement(42028..42633)
                     /locus_tag="ONT65_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2327782.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(43083..43703)
                     /locus_tag="ONT65_000120"
     CDS             complement(43083..43703)
                     /locus_tag="ONT65_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2327783.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43705..44574)
                     /locus_tag="ONT65_000121"
     CDS             complement(43705..44574)
                     /locus_tag="ONT65_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR2327784.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44597..45004)
                     /locus_tag="ONT65_000122"
     CDS             complement(44597..45004)
                     /locus_tag="ONT65_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2327785.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(45023..46312)
                     /locus_tag="ONT65_000123"
     CDS             complement(45023..46312)
                     /locus_tag="ONT65_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2327786.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46339..46641)
                     /locus_tag="ONT65_000124"
     CDS             complement(46339..46641)
                     /locus_tag="ONT65_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2327787.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46666..48759)
                     /locus_tag="ONT65_000125"
     CDS             complement(46666..48759)
                     /locus_tag="ONT65_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2327788.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49248..50012)
                     /locus_tag="ONT65_000126"
     CDS             complement(49248..50012)
                     /locus_tag="ONT65_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR2327789.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50123..50992)
                     /gene="mdcB"
                     /locus_tag="ONT65_000127"
     CDS             complement(50123..50992)
                     /gene="mdcB"
                     /locus_tag="ONT65_000127"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR2327790.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(51017..52093)
                     /locus_tag="ONT65_000128"
     CDS             complement(51017..52093)
                     /locus_tag="ONT65_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR2327791.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52117..52710)
                     /locus_tag="ONT65_000129"
     CDS             complement(52117..52710)
                     /locus_tag="ONT65_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR2327792.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52714..54390)
                     /gene="mdcD"
                     /locus_tag="ONT65_000130"
     CDS             complement(52714..54390)
                     /gene="mdcD"
                     /locus_tag="ONT65_000130"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR2327793.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54380..54679)
                     /locus_tag="ONT65_000131"
     CDS             complement(54380..54679)
                     /locus_tag="ONT65_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR2327794.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54781..56427)
                     /gene="mdcA"
                     /locus_tag="ONT65_000132"
     CDS             complement(54781..56427)
                     /gene="mdcA"
                     /locus_tag="ONT65_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR2327795.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56434..57345)
                     /locus_tag="ONT65_000133"
     CDS             complement(56434..57345)
                     /locus_tag="ONT65_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2327796.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57465..58322
                     /locus_tag="ONT65_000134"
     CDS             57465..58322
                     /locus_tag="ONT65_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2327797.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58509..58871)
                     /locus_tag="ONT65_000135"
     CDS             complement(58509..58871)
                     /locus_tag="ONT65_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR2327798.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59217..60656
                     /locus_tag="ONT65_000136"
     CDS             59217..60656
                     /locus_tag="ONT65_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2327799.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60663..63215)
                     /locus_tag="ONT65_000137"
     CDS             complement(60663..63215)
                     /locus_tag="ONT65_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR2327800.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63329..64660)
                     /locus_tag="ONT65_000138"
     CDS             complement(63329..64660)
                     /locus_tag="ONT65_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2327801.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64626..66104)
                     /locus_tag="ONT65_000139"
     CDS             complement(64626..66104)
                     /locus_tag="ONT65_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2327802.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66409..68244)
                     /gene="tdc"
                     /locus_tag="ONT65_000140"
     CDS             complement(66409..68244)
                     /gene="tdc"
                     /locus_tag="ONT65_000140"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2327803.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            68714..70141
                     /locus_tag="ONT65_000141"
     CDS             68714..70141
                     /locus_tag="ONT65_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2327804.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(70233..70598)
                     /locus_tag="ONT65_000142"
     CDS             complement(70233..70598)
                     /locus_tag="ONT65_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2327805.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(70595..70960)
                     /locus_tag="ONT65_000143"
     CDS             complement(70595..70960)
                     /locus_tag="ONT65_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2327806.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(71152..71400)
                     /locus_tag="ONT65_000144"
     CDS             complement(71152..71400)
                     /locus_tag="ONT65_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327807.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            71399..71527
                     /locus_tag="ONT65_000145"
     CDS             71399..71527
                     /locus_tag="ONT65_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR2327808.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            71584..73278
                     /locus_tag="ONT65_000146"
     CDS             71584..73278
                     /locus_tag="ONT65_000146"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR2327809.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            73282..73401
                     /locus_tag="ONT65_000147"
                     /pseudo
     CDS             73282..73401
                     /locus_tag="ONT65_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            73378..73551
                     /locus_tag="ONT65_000148"
     CDS             73378..73551
                     /locus_tag="ONT65_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327810.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            73929..75062
                     /locus_tag="ONT65_000149"
     CDS             73929..75062
                     /locus_tag="ONT65_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR2327811.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            75093..75413
                     /locus_tag="ONT65_000150"
     CDS             75093..75413
                     /locus_tag="ONT65_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR2327812.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(75442..75906)
                     /locus_tag="ONT65_000151"
     CDS             complement(75442..75906)
                     /locus_tag="ONT65_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2327813.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(76038..77291)
                     /locus_tag="ONT65_000152"
     CDS             complement(76038..77291)
                     /locus_tag="ONT65_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2327814.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(77320..77637)
                     /locus_tag="ONT65_000153"
     CDS             complement(77320..77637)
                     /locus_tag="ONT65_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2327815.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(77663..77983)
                     /locus_tag="ONT65_000154"
     CDS             complement(77663..77983)
                     /locus_tag="ONT65_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2327816.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(78447..79862)
                     /locus_tag="ONT65_000155"
     CDS             complement(78447..79862)
                     /locus_tag="ONT65_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2327817.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(79879..81195)
                     /locus_tag="ONT65_000156"
     CDS             complement(79879..81195)
                     /locus_tag="ONT65_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2327818.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(81345..82043)
                     /locus_tag="ONT65_000157"
     CDS             complement(81345..82043)
                     /locus_tag="ONT65_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR2327819.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(82332..83288)
                     /locus_tag="ONT65_000158"
     CDS             complement(82332..83288)
                     /locus_tag="ONT65_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2327820.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(83401..84234)
                     /gene="msrA"
                     /locus_tag="ONT65_000159"
     CDS             complement(83401..84234)
                     /gene="msrA"
                     /locus_tag="ONT65_000159"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2327821.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(84409..86043)
                     /locus_tag="ONT65_000160"
     CDS             complement(84409..86043)
                     /locus_tag="ONT65_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327822.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(86030..87415)
                     /locus_tag="ONT65_000161"
     CDS             complement(86030..87415)
                     /locus_tag="ONT65_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327823.1"
                     /translation="MCTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
BASE COUNT        25489 a        18318 c        14862 g        28746 t
ORIGIN      
        1 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
       61 tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
      121 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
      181 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
      241 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
      301 gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca
      361 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
      421 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
      481 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
      541 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
      601 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
      661 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
      721 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg
      781 aacagtcgta gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
      841 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
      901 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
      961 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
     1021 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
     1081 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
     1141 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
     1201 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
     1261 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
     1321 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
     1381 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
     1441 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
     1501 cagctgtacg atgatttttt tccaagcaga ttcgatcact gcagaaggtg cttggctttt
     1561 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
     1621 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
     1681 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg
     1741 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
     1801 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
     1861 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
     1921 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
     1981 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
     2041 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag
     2101 cacgatcatg gtcgtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg
     2161 tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag
     2221 gaaccaactt tattggacca atatgttact agctattcta tttgctatat tggcaatcct
     2281 gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat
     2341 ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat
     2401 cggaattagt ggctttctat tcggatggct tcctcgaatg ggtaaacttg cttatatcta
     2461 cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt
     2521 ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc
     2581 cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta
     2641 taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc
     2701 caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac
     2761 tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct
     2821 aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct
     2881 ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga
     2941 tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact
     3001 tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt
     3061 aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg
     3121 ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc
     3181 tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg
     3241 ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt
     3301 tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga
     3361 ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct
     3421 aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt
     3481 atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc
     3541 ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca attttttaaa
     3601 tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt
     3661 ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat
     3721 agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc
     3781 ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct
     3841 tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt
     3901 tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt
     3961 atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt
     4021 tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc
     4081 tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca
     4141 ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct
     4201 gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt
     4261 tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat tggtcgatca
     4321 aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt
     4381 attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca
     4441 agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt
     4501 aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg
     4561 ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca
     4621 agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca
     4681 atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc
     4741 ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat
     4801 caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg
     4861 gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc
     4921 tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca
     4981 tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt
     5041 tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt
     5101 tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt
     5161 ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca
     5221 tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt
     5281 ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca
     5341 ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag
     5401 taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct
     5461 caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc
     5521 ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga
     5581 ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca
     5641 cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat
     5701 tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag
     5761 ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca
     5821 aaactccttt tccactatcc gcttccttaa tagccgtctg aatctgatct ggagttggag
     5881 aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat
     5941 gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat
     6001 tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta
     6061 atccttccag catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat
     6121 tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt
     6181 ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga
     6241 taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg
     6301 ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct
     6361 acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc
     6421 acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga
     6481 ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg
     6541 ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa tttctaattt
     6601 tgggaagata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg
     6661 atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag
     6721 ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt
     6781 tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag
     6841 aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg
     6901 gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta
     6961 gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga
     7021 tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc
     7081 ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc tgcgactaac
     7141 acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt ttttggcaaa
     7201 gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt cgtgcttcca
     7261 gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg gaaactagct
     7321 gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc gctgatcttt
     7381 ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag ctttccagaa
     7441 gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg gatcttcccg
     7501 tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt tttctcaagt
     7561 tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc gattgctgct
     7621 tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc ttttctttta
     7681 ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt tattgatgta
     7741 tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga agtcatttct
     7801 gtttccgagg caccgttttc agcaccagaa gagcttatag ttgttgaagg ttctgtttga
     7861 gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact ggaaacagca
     7921 attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct tactactttt
     7981 taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca acaggctgtt
     8041 tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc catgtcccgc
     8101 ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc gttatcgcgt
     8161 tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc aattttcttc
     8221 gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc atttcaaagc
     8281 agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt ccttgatata
     8341 cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat attgaccaga
     8401 caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta aaaggatgga
     8461 gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc caacgttttc
     8521 tttgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata atctcaggag
     8581 caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg tcttctgtcg
     8641 ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac atcgtatttg
     8701 ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta atgatgccca
     8761 cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga ttttggacat
     8821 ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa taattcacat
     8881 acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa atcaatttcc
     8941 cttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt ttttcgatcc
     9001 gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct ggtgtttggt
     9061 ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat ttcgtcatca
     9121 aatactcgat tgtatcttcg atcacaattt cttcattatg ggctggcacc atgatcgtga
     9181 tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt ttgtataaaa
     9241 acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga taagaaacga
     9301 gaaaaaatac ataatagaca aacaagtatc taagaataaa ttacctgtat gggtgattga
     9361 gatcatttca gttccacccc attttcttta tacaaatctg caatgaatgt cgtatctaga
     9421 tttttctctt cggagacaga ataaaaacgg accgtttctc tctcttcttt tgttccaaaa
     9481 cgttccgtat acacttcttc aagtaattct tgtctttttt tcaatttttc atcttcatat
     9541 tcaaaagatt tatttacttt tttgtataga tttcgattgt tccacctagt caatacaaat
     9601 gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc cgattaagaa atacagcatt
     9661 ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg tctcgttaat
     9721 gggaaaaaga cccatataaa aggaagaaca acgatcatcc atcctaaaag agccactaac
     9781 gtttgccaaa tctttaacca gtaattacct tttgaaaaat acttatcttt caccaaaatt
     9841 gatgcaactt cttctcttgt tacttttttc atatcctcac ctcacgtttt tttgttacga
     9901 cgtaggagtt taccatacat ttcatttctt ttcacataaa gtgttcctaa atttttccta
     9961 agaaaaagaa caaaacattt ctcatcagca aatgaaaaat attttgttct ttttatcagt
    10021 tgttcttttt attttttatc tcttaattat gttgctcttt ccaatacgac aatgcttttc
    10081 attcctctag atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt
    10141 tcaaatggat ataagattac atgatcaaat tccagtcctt ttgctagact caaaggaatg
    10201 atttggcagt tttctaatcc atatgaattt tttttaatcg attcttgtag cgtttgggtt
    10261 tcctcttctg tttgggtgat aactgccgct ttttctcctt ttagagaaac caaaatctca
    10321 gctagtattt tcaacaattc ttcagaggat cgaaaagagc ggtattcggg ttcttttcct
    10381 tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca cagcataggt tttgaaaagc
    10441 tctgtgattg ctccgctaga acggtaacta tttctaagat cataccgatg gatcggcaga
    10501 tggtagtcgt cgaaacaacg cataatatca gcaaaagtag tggaagaatt aaagattgcc
    10561 tgattctcgt cacctactaa agtaaagtga gtttttggaa ataattcgat caatagacct
    10621 aattgtgcca gggtataatc ttgtacttca tcaatcaata gatagcgaaa tgctggtaca
    10681 tttacctttt caacaagtaa atgacgaata gctaatataa tgactgcttc atcaacagta
    10741 cgtgtgggtt gccaagcata agctgagcca tatcgttttt cataaattct ttcaaacaac
    10801 tgatgctcat ccacccatgc catttcttcg atttgtctgg ttatttttcg atattttttt
    10861 tgtaaaagtt tccttgcata agctacaata ctttgttccg aatcatcaga aatcaattta
    10921 ccgaataatt ccaactgcct atcttctgtc aacgagagta cctgattctg cacagcagga
    10981 ctttttgctt gtttcaaaag atgattcttc cattcgctca tcagtgcctt tttcgttgct
    11041 tgaagcctat cgatcaattt aggatgagca ggaactttct catatatttt ttcgatcgtt
    11101 tttttagaaa ggactgtttt tccattttta gtaagtccac ggataaaatt cggatgatct
    11161 aaaaacaaag gatctgattg ttttagatta tcgataaact ttttgctacg caaacgttct
    11221 gtctgctccg atacattttc tcccgtgata cgtttgaaat aagcctcttc accttctatt
    11281 tcctctgctg aaagctgtga aaccagctgt atgatggtta gattcagtgg atttctttca
    11341 cctaatgatg gtaggacatt cgaaatatag tcgataaacc gatgattagg tgatagaatc
    11401 agcaagtcat cggatgtcat cgtatctcga tataaataga gcagatacgc aatacgctgc
    11461 atgatagcag aggttttacc acttcctgct acgccattta ccaagatatt ttgacttttc
    11521 acatcccgaa tgattgtgtt ttgttccgat tggatcgtag cagtgatagc cttcatttca
    11581 ttggtctcat cttggcctaa gacttctaat aatacgtcat cttgaatagc tacagacgta
    11641 tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc tttaataacc
    11701 gtatcgattt tattttgacg aaccgtataa gaagatggac caatcatgtt attgtaaaaa
    11761 agttcggcaa ttggtgaacg ccaatcataa acaagatttt catctgcatc atctgtgaaa
    11821 tttgctgtac cgatataaaa ggattctttt ggctcattat ccagaaagtc tacaacgact
    11881 tttccaaagt agggactttt caataaacgt tcgatctttg ctagctgatt ttctgcgttt
    11941 ttctgtttga tattcatctg atcgatttct ctatttttca tttcaatcat cgaaaaagta
    12001 tccaggttat ctaagtaact gtcaaaattc aatcgaagct cgccagccat ttctttcaac
    12061 gactggtttc cttctatttt tgccgtatcc atcgattccc gcagttcgtt ttttttatca
    12121 attagctttt gatacacgtt cttcagatat gcttcttcta actgtttttg tgtgttgacc
    12181 atacacattc tcctcttttt ttaaagtggt ggacccgatt agtttacctc ttttaagagt
    12241 cgaatgcaag ctttttaacg agtatcattt gttaggatat atcagttctt caggtcggac
    12301 agtttgatat aaagaaaatg caccatcaag gttttttacg tggtagccag actgcttcaa
    12361 gattctttcc gctagatagc tacgtaatcc gctatgacag ctgacgatat attcttgttg
    12421 gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt
    12481 gaatttgccc ttttccaatt cttctgctgt tcgcacatct aataaaattt ttccttttgc
    12541 tagttctgtc ggcaattcat gccattgtac ggtttcacta agcccttctg caatgttcat
    12601 tgctgcatat cctagcatgt ttactggatc tttggctgag ccaaatggcg gtgcatacgt
    12661 caattctaat tccggtaaat cgaagatcgt caaatgccct ttgattgctg ttgctaaaat
    12721 atcgatccgt ttatccacac cttttgcccc aacaccttgt gcaccataaa tctcgccagt
    12781 ctctggatgg aagatcaact tcagtaaaat atctgttgct tctggataat agcttgcatg
    12841 atcttttcct gatacatgaa tgacttcaaa agacagttcg ttttctttgg ctgttctttc
    12901 acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt ataattgctg ttccaatacc
    12961 gccttgattt cttcgagaaa ttccggaaat cgtgtccgca acttgtctac cttgacgatt
    13021 ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg atttgctgtt ttacaacgat
    13081 agcatccccc accgcataaa tatcaggaat attcgtttga taacgttcgt ctactaggat
    13141 acccccgcgt atgcctaatt cgattccagc ggtcactgct agctggttat caggacgaac
    13201 accaatggat aaaataacta aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg
    13261 tttcccgtgg tcttcaaatc cagcgacagc atgagaagta atcacttcta ctccttgatg
    13321 agacaattct ttttctataa aagctgccat ttcttcatcc agtgttggaa gaatatgtgg
    13381 tgcttgttcg atcaccatga cttctaaacc tcttcttttt aggttttctg ccatttccaa
    13441 tccgatgaat cctgcaccaa cgatcacggc acgctttggc tgtttttcta atgcatgcat
    13501 cactttatcg atatctggta catttctaat agaaaaaaca ttgtcggctt ctgttatccc
    13561 tggaatcgat ggaacaactg gttttgcacc aggagataaa accaatgcat cgtactgttc
    13621 aatgtgtttt tgaccctcat gtacgacttc taccgttttg ttttcgggaa agatggctgt
    13681 cacttcatga tttggacgca catctaaaca aaaacgagcg gataatgatt ctggcgtttg
    13741 gacaagaaga ttttcccgtt cactgatttc tcccgaaaga taataaggca agccgcaatt
    13801 tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt tctgcatctt ccattaatcg
    13861 gcgaagccgt gtagcagcag acattccacc tgctacacct ccgacaataa cgattttcat
    13921 cttatttccc tcctactgtt cgatcaaacc attgattcat gccaccacgt acattcacta
    13981 catcataacc agatttcttt aattctttag ctgcttgttt actcctcatc cctgattgac
    14041 aaatcacata gacggtcttt tctttgtctc catcatatct gtttatactt tgtaatggaa
    14101 catttttagc tccttttata tgcccgcccc ggtattctgc aggtgttcta acatcaagta
    14161 acgctggatt ttccaaaagc ttttccttta attctgggac tgttatcgat tttattgatt
    14221 gaaatagcaa attcattttc tgcacctcat ttacattttt ataaatacct catggggtat
    14281 attaaacagg agctttatta atatgtcaac cagaatgact cttcattttt ttcactactt
    14341 tttatatttg cataaaatac cgataagggg tattatctaa ttataaaaat agaggagaat
    14401 gtgttatgga gattgataat aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa
    14461 ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc atcactcaat
    14521 taagcgctgt tcgttctagt attgatcgcg taatgggcat gatcgttgca gataatctga
    14581 aaaattgctt tgagaaacct gatagtaatc cagaagaaca agcggctaaa ctagaacaag
    14641 cgatcaagat gattattaaa aagtaaagga atgatttcaa tgaaagtcgt tttccatatt
    14701 gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct
    14761 ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta tctagactct
    14821 gccaatgcag aattcttaga tttgcaagga gtcgtttttc acgcttgcgc caatgctatg
    14881 cgtgctaatc atatttcaga aagttcttta cctgaacaag taatcgttgt accagcaggt
    14941 gtccttgatt tagttgaact gcaatcccaa ggttatgctt atatcaagcc ttagtcagta
    15001 gaggttgcga cagaagtgat cagcttcaag aaatttcggc ttatttttcg aagaagctac
    15061 ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag taataagaac
    15121 agccaaacaa aaatagcttc tcatattttt cgtttggttg ggcttattac cgcagatgca
    15181 agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc taagtattag
    15241 agaaaaaaac gggaaaatag ctcctcatat tttgccgttt tttgagctaa taccgaaggt
    15301 cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt
    15361 aagaaaaatt ttgtgaaaat ggatttctac atttttgcat aaatttggct taataccgag
    15421 aaactggctt ttgaataccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt
    15481 attagagaaa caaacggaaa aatagctgct catattttgc cgttttttga gctaataccg
    15541 aaggtcagct gattgaacac cgtttaccaa gaggttgtga gcctaccatt taactccgaa
    15601 tcacaagaaa taatttcaaa aacaacttct cctgtttttg gtttggttgg cttattatcg
    15661 agaagtagct actgggagtc tatttatcaa aaaaaggaat gcgacatagg tcacacccct
    15721 tttttatttt ctttgaaaca tctttcggta ctcgacagca tacaagacaa aagattgtaa
    15781 aagaccgtaa gtgatgacgg caaaaatgac tctcacccac agcgttggta attctgggaa
    15841 aagcagcaaa aggatcatgc caacatagat gacagcagtc gctactttgc cataccactt
    15901 cgctccactt aatctgatat ttttagatag cccgattaat ccagctacaa acataaagcc
    15961 ttcttttatc aagaatagac caaacaacag ccaaagccac ggtcgatacc atgccataga
    16021 caaaatcaga aaaagttgtg tcaatttatc tgcaaacgga tcaagcacct tccctaagtc
    16081 tgttacctga ttatatctcc gagcaatgac cccgtctaga taatcagaca aacctgagac
    16141 aacaatgacc gctcctgcta tataaaatga ctgtctcagc acaaaataaa gataaatcgg
    16201 cagcaaaaaa atacggaata tcgataaaag attcggaatc gtttgtattt ctttacgcca
    16261 gtctgtgctt aatttcattt tatcgcccta cctcctcagc actcttttct ttttatttta
    16321 gcttacacgg ggaataaaac caatgatttc actgttttta tttttcttta cagccactga
    16381 tattatgagc taatttgaga taatcacttg ctgttttcat cgtttactct tctttcttcc
    16441 tgtcgtttgc gcatttcttt tactagtact ttatgagctt tttttgatcc tcgctctagg
    16501 tcacgctgcc gtttacgtgt tttataatcg ataaacagac tgtctaaatc gtaacctgtc
    16561 gggtacagat cttctgctga acgcaccaga cgaacacgct gacgaggcac gtggattatt
    16621 tttttgtcta aatatacatc aatcgtatct tcacctatat cttcataaac taacgcttct
    16681 ttttggtatt cattgacaaa tatccgatca ccttttgcaa agagctggcg ttctttttta
    16741 ttctccttcc tatttgtaac tgctgtctta ggaagaaatt cttttttctg tgtccgatag
    16801 gctttttccg ccaagtaacg ctctgcttga tggatcaatt tcatcgacat atccatttta
    16861 tgagcaatcc acaaagcttg gctttctcct gtttctccaa cccgcaactg atatttagga
    16921 cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg
    16981 aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata
    17041 gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc
    17101 tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc
    17161 atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg
    17221 aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac
    17281 atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt
    17341 gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca
    17401 tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt
    17461 ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac
    17521 accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc
    17581 aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt
    17641 tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca
    17701 tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat
    17761 tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag
    17821 aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta
    17881 cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac
    17941 tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg
    18001 tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat
    18061 tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg
    18121 atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct
    18181 cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg
    18241 attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca
    18301 aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct
    18361 gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt
    18421 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
    18481 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
    18541 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
    18601 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
    18661 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
    18721 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
    18781 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
    18841 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
    18901 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
    18961 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
    19021 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
    19081 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
    19141 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
    19201 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
    19261 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
    19321 actctcccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
    19381 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
    19441 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
    19501 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
    19561 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
    19621 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
    19681 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
    19741 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
    19801 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
    19861 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
    19921 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
    19981 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
    20041 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
    20101 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
    20161 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
    20221 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
    20281 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
    20341 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
    20401 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
    20461 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
    20521 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
    20581 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
    20641 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
    20701 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
    20761 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
    20821 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
    20881 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
    20941 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
    21001 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
    21061 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
    21121 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
    21181 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
    21241 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
    21301 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
    21361 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
    21421 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
    21481 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
    21541 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
    21601 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
    21661 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
    21721 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
    21781 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
    21841 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
    21901 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
    21961 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
    22021 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
    22081 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
    22141 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
    22201 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
    22261 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
    22321 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
    22381 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
    22441 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
    22501 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
    22561 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
    22621 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
    22681 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
    22741 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
    22801 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
    22861 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
    22921 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
    22981 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
    23041 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
    23101 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
    23161 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
    23221 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
    23281 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
    23341 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
    23401 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
    23461 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
    23521 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
    23581 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
    23641 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
    23701 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
    23761 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
    23821 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
    23881 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
    23941 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
    24001 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
    24061 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
    24121 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
    24181 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
    24241 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
    24301 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
    24361 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
    24421 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
    24481 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
    24541 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
    24601 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
    24661 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
    24721 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
    24781 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
    24841 ttcttcccac atctgattgt cggggcgaga atgattagcc agcaaaacaa gatcatagtt
    24901 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
    24961 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
    25021 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
    25081 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
    25141 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
    25201 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
    25261 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
    25321 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
    25381 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
    25441 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
    25501 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
    25561 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
    25621 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
    25681 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
    25741 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
    25801 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
    25861 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
    25921 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
    25981 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
    26041 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
    26101 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
    26161 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
    26221 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
    26281 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
    26341 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
    26401 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
    26461 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
    26521 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
    26581 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
    26641 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
    26701 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
    26761 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
    26821 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
    26881 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
    26941 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
    27001 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
    27061 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
    27121 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
    27181 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
    27241 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
    27301 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
    27361 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
    27421 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
    27481 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
    27541 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
    27601 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
    27661 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
    27721 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
    27781 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
    27841 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
    27901 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
    27961 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
    28021 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
    28081 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
    28141 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
    28201 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
    28261 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
    28321 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
    28381 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    28441 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    28501 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    28561 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    28621 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    28681 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    28741 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    28801 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    28861 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    28921 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    28981 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    29041 atatgctgtt tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    29101 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    29161 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    29221 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    29281 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    29341 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    29401 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    29461 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    29521 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    29581 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    29641 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    29701 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    29761 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    29821 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    29881 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    29941 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    30001 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    30061 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    30121 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    30181 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    30241 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    30301 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    30361 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    30421 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    30481 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    30541 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacaggac
    30601 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    30661 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    30721 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    30781 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    30841 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    30901 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    30961 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    31021 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    31081 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    31141 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    31201 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    31261 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaag
    31321 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    31381 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    31441 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    31501 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
    31561 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    31621 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    31681 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    31741 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    31801 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    31861 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    31921 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    31981 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    32041 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    32101 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    32161 tctccattca attagtaccc cttataatag cgaacgaaac tgtacaatag tgtgtatggt
    32221 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    32281 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
    32341 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
    32401 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
    32461 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
    32521 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
    32581 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
    32641 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
    32701 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
    32761 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
    32821 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta
    32881 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    32941 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    33001 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    33061 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    33121 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    33181 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    33241 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    33301 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    33361 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    33421 aaattgtact gatttgttga ggcaaccttg cgtgttggat caccgtttct gttccaacaa
    33481 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    33541 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    33601 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    33661 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    33721 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    33781 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    33841 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    33901 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    33961 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    34021 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    34081 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    34141 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    34201 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    34261 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    34321 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    34381 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    34441 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    34501 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    34561 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    34621 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    34681 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    34741 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    34801 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    34861 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    34921 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    34981 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    35041 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    35101 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    35161 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    35221 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    35281 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    35341 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    35401 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    35461 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    35521 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    35581 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    35641 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    35701 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    35761 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    35821 cacaataacc cttaaagctt cacgtggaac agtgaacgtc tgcttttcac tggacatatt
    35881 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    35941 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    36001 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    36061 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    36121 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg cattgattga
    36181 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    36241 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    36301 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    36361 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    36421 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    36481 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    36541 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    36601 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    36661 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    36721 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    36781 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    36841 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    36901 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    36961 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    37021 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    37081 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    37141 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    37201 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    37261 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    37321 gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat
    37381 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    37441 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    37501 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    37561 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    37621 aaatatgagg aactattttt tcgttttttt ctctaatact caggccagaa cactgatttc
    37681 acaacctctt tataaacggt gttcactcag ctgtccctcg gtattagctc aaaaaacggc
    37741 aaaatataag aaactatttt ttcgtttttt tctctaatac tcaggccaga acgctgattt
    37801 cacaacctct ttatttcaca ttcaatatcc acatgaatcc cacaaataca aagaacagac
    37861 catacatgat tggagatact tcttttcctc ttttagctgc taacatggta attggataga
    37921 agataaaacc taatgcaatc ccatctgaaa tactataagt caaaggcatg ccgatcaaga
    37981 tcaagaatga agggatcgca atctctaagt ttccccattc gatttgtcgc aaagattgtg
    38041 ccatcaatac tcctaccata atcaatgctg gtgcagttac ttgtgaagta acaactgata
    38101 gtaatggaga aaagaacaag cctaacaaga agaaaatccc tgtagtaata gctgtgatac
    38161 ctgaacgtcc tccaacggcg ataccagctg atgattctac aaatgcacca acaggagaag
    38221 taccaaataa ggaacccgca agcatagctg ttgaatcaga agctaaagct ttccctacac
    38281 gaggcatctt gttgtctttc atgaaacctg cttggtttgc taatccaact aaagttcctg
    38341 ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac gacccacatt tgaagagaat
    38401 tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg
    38461 caacaatttc agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg
    38521 ctatcccgat gaagatgcca cctggtacac gccgaacaag caagatagca gttacgacta
    38581 atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct actaatgtag
    38641 attcattcga aacgatgatc ccaccttcgc tcagaccaag gaaagcgata aatagaccga
    38701 taccgccaga aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc atttcccgca
    38761 gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg gctaatgctg
    38821 tttcccatgg aatccccatt cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa
    38881 gagcaggtgc cgttccaatt ggataacggg ctaatacccc cattaaaaca cagccgatcg
    38941 cactggctaa tgcagtcgca gtaaacactg ctccttcatc cattccagaa gcacctaata
    39001 cagttggatt tacaaataag atataagcca ttgagataaa tgttgtaaat cctgccagta
    39061 cctcacgttt taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt attttttcct
    39121 tcatcttcca tccccctaaa tccgaacatt ataaataaag tccgttaaaa tttcgattta
    39181 ttaataaaac aacgtgtttt atcatatact aaaacaaaac ataagtaaac aaaaaattcg
    39241 gattttaagc aaaatccgaa cgttccatct gattaaatcc ctttatctca aagttcatga
    39301 acattcactt tggattacaa gaatttcttt attgtgttaa taaagtttct tcattctagt
    39361 ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt tctgttcttc tttgatgaat
    39421 tcttttgtat cgacaggatt cagattattt agtccgccaa aacctgtatc gaccattttt
    39481 ccccagcctt ttgtcttctt ttttcctgat ttgagattct ttgctcgaag cttctcacta
    39541 tgttttgctc gccgatactt caaataacct tctaatcttc ccattagatc cactgttttt
    39601 cctccttttt attcaatcag acgactcagc ttttcctata acaaaataat tgccttcagg
    39661 gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg atttctcctg gttcttccat
    39721 gttctcgtag atagtttcaa attcatccgt aaaaaatact aaagaaggcg gtggtcctaa
    39781 tacctctggt gaaaacttct gcacaaatac ttttgggaat acgcctaatt ctaattcttt
    39841 tgagatgctg agtacaatac tcttaaattc ttctggcaat tcgattgttt cagaaatttc
    39901 tgcaccaatt ttttctacaa agaatcggca aatcatctct acgtcattga cataaagcat
    39961 cacacgtgtt ttttgtatca tcatttgacc cttctttcta tttgattttc tttcctgtct
    40021 ataaactgat tccattatat cacgaaagcg caaacacctt gtgtttgata ctattcaaga
    40081 aaaataaagc aatagtttct aggattgctt tcctccacaa acttttttta caaaaaaaaa
    40141 aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca
    40201 tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc
    40261 aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc
    40321 agcaccgctg cttgtgtttc ttccgtcagc attgttcttt gggcttcacg ccagttgaga
    40381 cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca
    40441 tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga
    40501 tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata
    40561 ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt
    40621 ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc
    40681 tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt
    40741 gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg
    40801 tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag
    40861 aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct
    40921 tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt
    40981 tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga
    41041 gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg
    41101 taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat
    41161 gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct
    41221 cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt
    41281 tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc
    41341 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
    41401 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
    41461 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
    41521 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
    41581 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
    41641 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
    41701 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
    41761 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
    41821 ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa
    41881 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg
    41941 ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta
    42001 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
    42061 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
    42121 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
    42181 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
    42241 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
    42301 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
    42361 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
    42421 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
    42481 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
    42541 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
    42601 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
    42661 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
    42721 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
    42781 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa
    42841 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
    42901 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
    42961 tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg
    43021 ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt
    43081 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
    43141 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
    43201 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
    43261 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
    43321 tcacgatcac gtttgtaccg acgttgacat aacacttcaa agtctgctgt taggcgaatc
    43381 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
    43441 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
    43501 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
    43561 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
    43621 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
    43681 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
    43741 cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac
    43801 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
    43861 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
    43921 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
    43981 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
    44041 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
    44101 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
    44161 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
    44221 tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga
    44281 agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
    44341 caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
    44401 catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc
    44461 ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac
    44521 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
    44581 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
    44641 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
    44701 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
    44761 tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa
    44821 atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa
    44881 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
    44941 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
    45001 acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat
    45061 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg
    45121 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
    45181 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
    45241 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
    45301 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg
    45361 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
    45421 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
    45481 atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
    45541 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
    45601 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
    45661 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
    45721 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
    45781 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
    45841 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
    45901 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
    45961 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
    46021 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
    46081 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
    46141 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
    46201 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
    46261 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtttttac
    46321 cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc
    46381 ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac
    46441 tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat
    46501 ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa
    46561 aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcac cgcatacgaa
    46621 taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac
    46681 tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt
    46741 ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatgcg agtaggagtc
    46801 tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat
    46861 tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc
    46921 atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg
    46981 aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc
    47041 tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt
    47101 caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc
    47161 gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat
    47221 gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg
    47281 tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat
    47341 cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag
    47401 attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc
    47461 gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg
    47521 gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat
    47581 cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac
    47641 ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg
    47701 gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc
    47761 agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga aaggtacgtg
    47821 tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc
    47881 gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct
    47941 ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga
    48001 aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt
    48061 cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc
    48121 ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt ccaccaaagg
    48181 aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa
    48241 gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc
    48301 tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac
    48361 cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc
    48421 ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc
    48481 aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa
    48541 tcgagcttgc ttttcctttt ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat
    48601 ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct gacggatcgt
    48661 ccgctgactg acattgaact cacttgctag atgttttgct tttactggat attgggctgt
    48721 catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt
    48781 tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa
    48841 agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa
    48901 agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa
    48961 agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc tatttttcgt
    49021 ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg agaggttgtg
    49081 acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt
    49141 acaaaatcgt gccttattgc cgataggcta tctctggcac actgtttatt cagaaaaagg
    49201 agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata
    49261 atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat
    49321 atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga
    49381 tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc
    49441 tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct
    49501 gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt
    49561 ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg
    49621 atttccagaa actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc
    49681 gctttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct
    49741 actgacaaaa cagctaatgc aagaaatccc gtttgctcat aactagaagg acctgtacct
    49801 ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata actgccccca
    49861 tatgaacgac caccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca
    49921 tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac
    49981 aaaatgattg ctaaataatt gatttttttc attgtcttcc tcgttatctt ttattacaaa
    50041 aaactatttt tatgatagcc ttgctaaaag gaagagtcaa taaccagatg cataaactgt
    50101 ttttttagca attttgtctt atttagtcat tgagtaacat cggacaaatg tatgtgtcgg
    50161 cagtccataa ttcctctatc ttcagcatga acaagcttgc agacaagaga tctgcgcttc
    50221 ctccaggaga gatttggttt tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg
    50281 gtaaagcact ttgactagca gcatgagcaa gtttttgcat ttccactaac gttttctgat
    50341 cactgcgatg caatacgcat gtgtcatcta gcgaactcat caaaactaac aacatatgca
    50401 tttgtttctg tacatatgaa tcattatcta gctccttagc tgcttggatt gcaagcctga
    50461 tatgcggata ccctaatatc gcttcttcat atgcaccatt tacgccatat ttttgcttca
    50521 ccctatgacc atgagtggtt tttttatgac gatattttgt atcaggaaaa gcagcaagct
    50581 tgctgacatc atcaagtatt ttcgataaag aaaaaggctg ttccatactg attttttgtg
    50641 caatgacact gatcaatagc cccattgccc aaatcgctcc tttatgggta ttgatgccat
    50701 ttgtagcaat cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt ctttctctta
    50761 actcctgatc aatagcatga tgccatgcta tttgagccat ttcttcaaaa aaaggagtta
    50821 gcgtcttagc agacttaaga aataaagcat agtccatatc ggaatgtgca cccgcatcca
    50881 gtgcatccac taatccaggt tttggactta atgttacttc tgcgatcaat gcttcctcta
    50941 ccaacgcagc gagttgctga gtcactgctt tttttcgttc gtcatttatc atgacactca
    51001 ctccttcttc ctactattac ttgctgaaac cttttttgat agctacccaa atgatatgaa
    51061 gtaaccagct taaagcaaat gcacaacttg ccgtcattag ataagctaca agaatcatga
    51121 ctcctgaagg taactgcagc caactatctt taaacaataa taacagtact tggaaaacaa
    51181 atacattggc taaatacgta cgataagcat aaacagacaa ccatttgaac aatggcaatt
    51241 ttggcgaatc gtttacaatc attttggaag cgcctatgaa gacagcaaag atgatgacta
    51301 aactgtataa actttggatc gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg
    51361 gatgtgcgag caatgaacgg acagaaaaga acgcgataac taaggcaaca ggtaagaaaa
    51421 gataacgtac tctgtccaaa tattgataga ctttttcgtg ataaacaaat gcggctaccc
    51481 cataaatcat gaaactagca acaaaccgat ctaataagta ccagctgtca tgataaggcc
    51541 cagtcaatac ttgtgtttga tagaacagat accatgcgac atataaaatc gttgaaccaa
    51601 tcaatactgg ccatacatat tttttattag caaatacttt gtaaccgagc caaacaaaaa
    51661 acggcatgaa caattggatc tgcagcatca taacggtata ccataaatga ggcgctccat
    51721 cacctgtgac atatttcagt aaaaagcttg cagtatccgt atacggagtt tgttgttgga
    51781 ctgctggaaa tagccaaaga taagccgtcg tccatagaag atacggaacg actagttcaa
    51841 aaaacttttc tttcaaaaat tcccaataag acaaatgctg acttgttttt accatattat
    51901 aaacaattgc aaaaataaac attggtgctg tgtatttaac gatgacgtaa aaggtaccaa
    51961 gaaaagctgt atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca
    52021 tcataacagc cgttacagct gatactttct ggaagtcact tacgttgatt ttgatttttt
    52081 tgttttcaaa catctctttc tctctctttt tctttattac caaagttctt tggttaattt
    52141 aggtccttcg actgttttca atagaatttt tttctctggc tctctcaaat actcttttag
    52201 tgaaaaaccg ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg
    52261 cgattgaatt acctgaattg gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa
    52321 atcactggtt tcttttactg ccggaatccc agtcgataat tcaaatccta agctgccgcc
    52381 gattccccat ttacatcctg ataatatgtt tctagcagca tgaaattgct gataaacagg
    52441 ataatccatg attttctctt gtctatacga ttcttgttcg atgatgtccc acggatgaat
    52501 gacttgtttg acggcatgtt ttgagatttc tgttgaccat ctctgattgc gggcaggtcc
    52561 acgtaaacca atcggtagta tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa
    52621 catcttttca gttagaacga cccattcagg caaggtgaac ccttctattt catgtttatc
    52681 aaaaaggaca atatcatgtg cttgaatcat tttttatgca tcccattctt cgtccatttt
    52741 ggcacggact ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg
    52801 atttgtcagc atcgtgtcat ttgtattgcg gacatcttga atggcttcat tgattgcttc
    52861 gatgactttg ctgacatttt gttctgaagc agcttggtcg gtgatatctt ctaggaaatg
    52921 atagatcgct cctagttttg tataattatt gatatcgtaa gcgatagctg gaactttttc
    52981 agcagctgct tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac tttctttaga
    53041 catcgcttgg acggtaattt ctttattgtt taaagcaatc aagcggcttg attgtaatcc
    53101 atgtgctaaa aatgcacctg aaatagcatt tcctgcaatc aatccaataa ctggatgtcc
    53161 agcttgacgg agttttgcat aagcagcagc actattagcc aaagccacat ggataccgat
    53221 caattcttcg ttatagccgt atgcttggct tggtacatcg atcacaacga tgattggccg
    53281 tttcactttt ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa
    53341 accttcaact aaaccaactt caccgtttcg tacacggtac atcgggtttt taggatcagg
    53401 tacgattgat gtcacgatgg cttcttctat gaatttactt gatttaccat aatacacagt
    53461 actaatagaa gaagttggat ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac
    53521 acttgctgca gctggttcat ttccttctgc tacttctggt aagtcaattg attttgcttg
    53581 atggttagca attgcttcat tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga
    53641 atcaagtaat gataaataaa atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc
    53701 cattgctgcg atgacagcag ctttgattgc ttctacacta tcagtcacta attcatcaat
    53761 gatttttgat tctacccgtt gacgagatcc taatgtattc caaatcaagt ctttgtcact
    53821 agaatcaaat tctcgaacgc ctgcttcttg ttcaataact tctggtccat tcaaaccgac
    53881 tctcgctttt tttgttgcga ttaaataact cattaatgct gaagtaatcg acataccgcc
    53941 aaatgatcct acacgtcctg gaatcaaacc gatgactggt acatattgtt ttagagcaat
    54001 gattacattt ccgatttctg aaatagataa cagtccgtag tttgcttctt gcaaacgaac
    54061 accacctgta tctagtacaa tgactggaaa gatctcattt ccttttttat tttcttctaa
    54121 cacatgttca agtgcagcaa cgatttttgc accggaaact tcaccgatac cgccaccttg
    54181 gaattttcct tccatagcga ttactactgc ttgtttgcca ttgatcgtgc cacggctgac
    54241 aacgatcccg tcatcacttt cagggacgat tccttgtgct ggtaaacttg gcgcgatgat
    54301 ttgatcgaat ggatcaatca attcacgtcc attgtcaagt agagcgttca ctcgttctct
    54361 ggcgtttaat tctacaaaac tatttttcat ctgaaagttc ctccattgct tgcgtcaatc
    54421 gtaggtacac aactcctggt gtagcaccga agtcattgat cacgatatct gctgaaattg
    54481 gataacgatc aaaaaatctc gttaatacat tgttccatac ttcgccgaag ccatcgcttc
    54541 ctgtacaaat ttttacagta gattctgttt tttctgttgg aaacatcaag atttccaaat
    54601 ctcctgaacc gaccactcct acatggattt tcttattgat cggctgtttc gttggatatt
    54661 tataagctaa ttgttccatg gatctctccc ctttttgtaa aacagagcat acacgttctg
    54721 cttattcgtc tttcttcttc catctgggta aatggcgaca aaaggttttt ctcgccaatt
    54781 ttatgaccaa gttctgaatt tcattggtgg ttcatacagg ccatctgacc attcgatcaa
    54841 atcatcgatg gtttgtgcag ctaataatga acgtttcgct tcactacgtt tgatgcctag
    54901 atcttctggt aatttcacga tgccggctgc acgcaagcta tctagtgatt ctttcgtgct
    54961 agtcattcca agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc gttcttcttt
    55021 tgtttttgct ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg tagtatcttc
    55081 gctataaatc atcactggtg cattggctaa gcctgcttgt ttttgaacag ctagtgcatc
    55141 taattcttcg acaaagactg gttttttatt tgaaccatat gtttcaacca tttgaacaac
    55201 taatttttgg cctttcccta atggatcatc attgtcgcga agtgattgcc atgctggtgt
    55261 agaatgacga cgaccgccag gattatgtcc catgttaggc gcaccgccaa aaccagataa
    55321 acgtcctttt gttacggtag aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat
    55381 aaacatatcg atggcatatt gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt
    55441 gccgtcttta ccgacaaaaa agacatctgg gcgagcagca atatattttt ccataccaac
    55501 ttcgccacca aaactatgga tgctttcaac ccaacctgat tcgatggctg gaatcatcgt
    55561 tggatgtggg ttcaataccc agttcttcgc gattttccct tttaacccta gtgattcacc
    55621 ataagttggc agtaacaatt cgatagctgc tgtattgaat ccaataccgt gattcaaaga
    55681 ttgtacgccg tgttctgcgt agattccttt gattgccatc attgccatta gaatttgcaa
    55741 ttctgtgatg ttttgaggat ctcgtgtgaa taaagcttct aattgataag gtttatctgc
    55801 tttcacgaca acgtctaccc agtcaccagg gatatctaca cgtggtaaag aatcgacaac
    55861 ttcgttgact tgaacgatca cgataccatc tttgaaagct gcagcttcga tgattgttgg
    55921 tgtttcttcc gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc
    55981 tactaaaaca acatttggaa tcaaatcgat gaataagcgt ccataaagtt ccaaataagt
    56041 atggatatca cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg
    56101 tgcaccagca taagagaaat cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg
    56161 ttctggtcgt gagatactag acatgatcaa atgcagatca ttcacttttt ctgcatctac
    56221 tttttcaaga ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac
    56281 tttgtcgccg ggagcaatca gtttttccat tacttctacg attttttcag tttctacata
    56341 cttgccattg agcaagttgc tgattttttc caatcgctgc gctttttttt cgcgtttaga
    56401 agcccagttg cggtttggtt tttccataat tagtcatctc catttttgat atagaaacgt
    56461 ggcgtcatca ataccatgtt ggtctagatt gatcgtacgg atccctttat ctacaccaaa
    56521 ttttgtatga atcagctttg tcaaagtatt gccgggggga aattcaatcg tcagatccat
    56581 tcctaattct tttgccaagt ccatcatttg gttccactgc acagggaaag ctaggttttc
    56641 caacaaatct ttacgaatct cttctgcgtc ctgagtggct cttccatccg tattttttat
    56701 atacaaacat ctagcatcag atagatggac agactgcata tacggcttga atttcttgac
    56761 cgtatcattc atcaagagac agtgagatgg cacagggact ttcagcagct tcgctgtttg
    56821 cgcttggtgc cgtttggctt ctgccaacgt aacggataaa ccttcgatac tcccagataa
    56881 cgtatgttgc atcggacaat tttcatttga cagataaact ggatggtcag gatcaaatgt
    56941 ttttgctaca atttcctcag cttcgcttct cgtcacacct actactacgc ccattgcata
    57001 accgcttgga taagcttctt tcattaagac tgcgcggtga tagactagtt ccgctgcatt
    57061 ctcaaatctc aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc
    57121 tgcaatatca ggctgaatac cgtttttttc catttcatct aaataaaata ctgcttttgt
    57181 cagtaatgct agctggataa aaaccgtatc ttgatagttt tcttctttat caactaaccg
    57241 ataccccgtc acattttcta aacgttccat gtacatttca gggatatccg ctaacatccc
    57301 taacttctga ctgccttgtc caggaaataa gaaacctgta ctcatgagcc tcgcctcctc
    57361 agcacttact ttttccactg ctatcttagt caatttctgc tataataaaa aatgccaaaa
    57421 aaagaaaaaa ccataacaaa aaggtatgga tgggatggat aattatgaaa ttgcttcatc
    57481 tgcagtattt taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat
    57541 ttatttctca acctgccttg agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc
    57601 ctttgtttta ccatagcggc agaaacattg agacgaccgt ttatgcagaa gaattttatc
    57661 catatgcagt caagacatta gacacattag aagaaggaat caaattattg tcttcggtca
    57721 atgagaagat cgttacttcc gtcatattgt atttagaggt agcctctgtt tctattccga
    57781 acttggtccg aatttttatg gaaaagcatc ctgatatcca attaacgatc atgcaacatc
    57841 atttaccaga tgatattacc gatcctgtac tgtacatcac ttcggagatg aagcctggca
    57901 tgaccaatat tcctatcatg aaagaacctg tttatgtcgc cattcctaag aaacatcctc
    57961 ttgctaaaaa agaacatctg caattagaag atatccaacg cacccccatc ttgatgctgt
    58021 caaagagcaa cgccttccgt catacactgg atgcagcact tgaagcgaaa agtatcgaat
    58081 tgacgatcag ttcgacgaca gatgatcctg cgacattacg ttctattgtc aagcaaggat
    58141 tagctgttag ctttttccca aaagtttctt ggtcttacga taaaaatgat cctttcgtct
    58201 tgcgggaaat caaagatttg cccttgacac gaacaattta tttatcttct agcttcacag
    58261 aagatcatcc tttgatcaaa atgattgcaa aaacgatgcg ggaattttat ttaggaaaat
    58321 aaaaaaaaaa gagctgggac aaaattcttg tcgcagctct tttttcgaac aaacggtgtt
    58381 ccagaagtaa tttcttagaa aataagccga aatttcccaa aaatttgaga agcaattttc
    58441 gtaaattcct tcttatttct tgaagctaaa catttctgtc acaacctctt ctctattgtc
    58501 ctagacattc attgcgttga tttaaaactt ccatcaatca aatctgctag ttcatctagc
    58561 ttttctattt tacgtagatc aatagagatc caatgttctt tattcatgtg ataggctgga
    58621 aaatacccct cttttcctcg caaaattccg ccaagttcag gatcgacctt caaatcaaga
    58681 atattgatga tcctatggtc acttgcatca tcatagattt tttcttctgg aacagacatc
    58741 aggaccccaa accatttttt gcttttttga tgtctgaata cgcaataatc aggaaatttt
    58801 ttaaaaatat attctggaga caaattgtat gtttcattga tgtgatcgat tagttctttt
    58861 ttcgttagca tctgcttcca tctctttctt attgattcgc tttattcaaa gataccacgt
    58921 aagaatagaa aaatgaatac aagtagttgc taatcaaata cttcgatatt tttctttttt
    58981 atcaactctt aaatgttcaa tccgcctgtg ttggtcttgc taccatctac tattaaaatg
    59041 tttattattg tttgcccacc tattttttga ttttagtggt atcatttttc aagttcatga
    59101 tgaaattctt atcatcagta aatgctccag aaaaataaac aatgattgaa aaatcagttg
    59161 aaacttgacc accgtttatt ttctccgtat tcttagaata gaagggagtc tgattgatgt
    59221 attcactaat gaaaaaaatc attaccgaaa aagatattcg acgtcatgtt tctttagtag
    59281 aacaattgct gaatcataca aagataaccg taaatgaatt agctgaaatc atagggacaa
    59341 ccgaacgaac gattttttct gaccttcaat ctattcgctc tcagctgccc gaaggctggg
    59401 acatcatcag cgatcaagca ggaatcagtt tgcaaaacca gcaaaattta ttaacaaatg
    59461 acctttggga gatttttttc aaacaatcag tcagtgtcga actgctgaaa aatctgcttt
    59521 ttacaaaaaa agttgctgtc cctgattttc tagccgacca tgggctttct tatgggacat
    59581 tgaaaagaca tgtgactaaa atcaatcagc gtttagcaag ttacgacttg cagatcgatt
    59641 tgacgaaata tacagcttgt attcttggaa aagagcgtgc gattcgtact ttttatcatc
    59701 gattgctgat tcctttcact cataacaact attttttcga ggattattcg atccatgaat
    59761 cccattattt tcaattttta agaaacctca gtcaaactga actagcggtt gaaacagaag
    59821 agattttcgg aacatgttgg ttctttatca atacgatccg tataaaagcg aattgccgat
    59881 tagattcttc tatccatacc aacagcacac tctcctccct gtatgactct gcattgaaaa
    59941 aattgtattt aacggaaggt atttatctta aagacactga gctttctttt gcttctttct
    60001 gctttctgga aagttggaat tacaacaata actatggaca agaaaccgct cgctgcctgc
    60061 atcattctcc atttttagag gtcctcgaaa cgtttgtaga ggaactagct tcagagctgt
    60121 ctcttgatca actaaaaaaa acatctctga ctgataactt ggctctcctc atattaaaat
    60181 atcatgaatc gccgatattg attgaagcat tggaccgtca ataccatcgt tttttagaaa
    60241 catatgatca gcaatatgca cacttgtatc aacagaaaga gaatttactg aacgatctaa
    60301 aaaaagaaat agaaatcaat gaaccaaatt actttctcca actcttgtct ctactgatcc
    60361 aacaaacgat tttttctatc aaaccgaatt tgttcaatgt ctattttatt ttccaaggag
    60421 aaccttcatg gaaggcgttc ttgcagcagg aattaaaaga ttacttagga aaacgtgtct
    60481 gcttcttacc tatcgaacta accgatcttt ccactatttc ttttgaaaaa acagatatct
    60541 tgatttccaa tttccctcta gattctctag atatccctat tgtatacatt tcttctattc
    60601 caacgaaaaa tgagctcaat cgtttgactg aactcacttt caaaactttt ttatagtaac
    60661 ggctagcgac tgtattctct agctgttttt tcttttttgc gaaacagttt gttcatgatc
    60721 cctttttcta tttctacaac taagaataga agaatcacat ttgctccaat caataactgt
    60781 tgtgtcatcg ttaaacttac tgtaccgatc agctgctggc cgactggtag aaaaataacc
    60841 atcccttgca ataaagcaag gatacctaat gaaagaaaaa gcgctttgtt ttgcaaaaga
    60901 ccttggttca atgacggctt ggacaactct ctgcaattga tcatgtaaac agcttgagct
    60961 aagacgatat tttgcagaag aatggtttgc tgtaagcttt gcccttcaaa ctgcatagcc
    61021 aataaatagg caggaaccat gatcagtagg gagacataga tgatccgaaa caaatgataa
    61081 ccagatagga ttccttgctt tggatctctt ggtgggcgct tcatgatttc tggatctggc
    61141 gactcgaacc ctagtgcata agataaagtg attgtcgtta ccatattgac ccataaaatc
    61201 tgtactgggg acaaaggtag cgggcgattc agcataagtg cccaaatcaa gatcaatcct
    61261 tgagctaatg cagttggcaa gaagaaattg atggtttttt gcaaattatc caaaatccgc
    61321 cggccctctt ttacggcttt tgcaatcgta tggaaattat catctcctaa aaccatatca
    61381 gctgcctgtt tgcttacttc actacctttg attcccatag caattcctac atcggatctt
    61441 ttcaaagcag gtgcatcatt tacgccgtct ccggtcattc cgacgatttc tccatttttc
    61501 tgcaaagctt taacaattcg cagtttatgt tctggtgtcg ttcgagcaaa gacatcgact
    61561 attggcactt gctgaatcaa ctcttcatca gacataagat ccaaatcgat cccttctaaa
    61621 actttggcag tatgttttaa gccaatttgt tccgcaatcg cttttgctgt gtctttgtga
    61681 tcccctgtaa tcattttcac tgagatccct gcttgttggc attcctttac agctttgact
    61741 gcactttctt ttggcggatc gatcaatccg gcgatccctg caaaagttaa ttgggttaaa
    61801 gtctcatgcg tgagttcctg tttcgaatct acttttttat aagcaaatcc taaaacacgc
    61861 tgaccttttc tcgcaaattc tgcagctgtc tgactccatg cttcttgttc tgcagaagaa
    61921 agagttgatt tagcaaataa tacttctggt gcacctttga cgtaaatcac cgctcctttt
    61981 tcctccgcat gcatcgttgc catatatttg tattcagaac tgaacggaat ctttgcttct
    62041 atttttttca aaggtagatt ggctgtttct gtgtgtcgaa tcagcgcaac ttctgtcgga
    62101 ttgccactta aattttctgt tttctgttct tcttttgttt ttaattcctg acagtttttc
    62161 ataatggaca acacgcaggc ttcttctttt gcagctacat ccacaactgt catttcattt
    62221 tttgtcaatg ttcctgtctt atctgaacag atcactgtca tagaacctaa tgtttctacc
    62281 gaaggaagtc ctttgatgat cgccttttct tttgccattt catgtactcc aacggataga
    62341 atcatggtca aaacagctgg caatccttcg ggtaccattg acaccaccaa agcaatcgta
    62401 gccgaaagaa ggatatgcca atccatacca tatcgaaatg tcgtaaaaat aactaagaat
    62461 aggctcaaac aaagaatgcc ttgaaaaatt tgtttgttta atcggttgat ttttttaatc
    62521 agaggcgtcg tttgttgttc gaccgattgc aatgcttgat tgattttacc gatttctgtc
    62581 gtgtttcctg tttctacaac gacaccgact gcagaaccag attgtacaag cgttcctgaa
    62641 aaagccatat ttttctggtc gccgatcgat tcttcttctg tcaaaacggc atgacttttt
    62701 tcagatgctt ctgattctcc cgtcaaaatc gattcttcca ctaataaatc atgtgcttcg
    62761 attaatcgga tatctgctgg aacgacatct ccggcacgta agacaatgac atctcctttt
    62821 accagttctt tcgcagggat tttttcttgc atcccgtttg atagtactat cgcttcttgt
    62881 cccatcagct gcttcaaacc attcaatgat tcttccgctt ttctttcttg ccagtaacta
    62941 acgagtccat tgacgatcac tacgaataaa ataatacttc cttcaatata ttcactagaa
    63001 gcaaatttca aaaaagaggc aatgatcaac acgatcatca gcagatcagt aaaatgcttt
    63061 gctatttttt tccatcgttt tgtctgctgc cttgctttta tttcattata tccttgatca
    63121 gtcaggcgtt tctgtcgttc ttcttgcgac aagccttctg ttgatgtttg atagtttttc
    63181 aatacatctt ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt ttaattgata
    63241 ctgttattat acgcagggaa gttttttcag gcgtatcgat tatttcaggg gggtctgaaa
    63301 taattcgttt tgcaaaatgt atctgggcct aactgatatt tctattgctt attcttttat
    63361 atacaaaatt taagaataca aaccagataa ccaataaaaa aggaataaag cgtgtcgtgc
    63421 tttcgaataa caagctgaaa gcaattgaag taaaaaacac caacaaagca agatctttat
    63481 atctttgtgt agtgttccgt ttcttttctt taataaattg aacatgtgca cacaagatcg
    63541 aagaccacac aaatagaaaa caactcgttg tcatgcctgc caaaagctga aaaaacaatc
    63601 gactatcagc tacaaaaaaa tgtagacaaa gaccggcaaa aaataacatt cctgtaatta
    63661 aaataccgtg taaaggtacg cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt
    63721 tttcttttgc tgtttgatat aaaatcctgc tagtactgta gactgcacta tttgctgagg
    63781 acaaagaagc aaccaaaatc attagattga caattgctgc tccgccttgg caatggatca
    63841 cttgcagcat agtcactaaa ggactttctt ttaaatctaa acaatgccat ggaacaacaa
    63901 ataaaatcga caaaatcgtt cctatataga aaaagagaat acgccaaggc agacagttga
    63961 ttgctttttt caaattcttt tgcggatcac ttgtctcgcc aacggttaag ccgatcattt
    64021 caattccagc aaaactgaat aaaaccatag gaaatgagac taccattcca gcaaagccaa
    64081 acggtagtaa ttcatcgatc tgtccaaaat tttctttgat tccgatagac tgggtatgaa
    64141 aatctgagac actcaatcct atacccaata agataaaaag gaaaatcccc aagatcttga
    64201 tgattgagaa aaaagattcc aattcaccaa accacttgac tgcaacaagg ttgactgcca
    64261 ttaagatcat tagtgcgcta cctccgctta tcaaagctgg tacatgtaca aaccagaaat
    64321 ggatatacaa tcctattgct gttaattccg caagtccagc tccaagccag caaagccaat
    64381 aagtccaatt gacaaaaaat gcccactttt tgcctaaata atagtcaacg aaatcactga
    64441 tagagttaaa tgtactattt gctaaaaata aatcacccat ggcccgcatc aaaccataaa
    64501 tagccaaacc gcatatcaaa taaacgagaa taacagctgg ccctgctgat gcaattgcct
    64561 ttgctgaacc taaaaataat cctgttccaa ttgcgccacc taatgcgatt aactggatat
    64621 ttcgattagt tagagtccgt ttcattgccg gtttttccat caagttcacc tttttctttt
    64681 tcaagcagca tggcgacttc ctgttcccaa gaaggtttct ttattttttc aatacagagt
    64741 ggtactacaa acaaaatgac actgatggct gtgactgcaa ttagattgat ccatgttcca
    64801 gctttcatcg tcagtccgac agaaaatccg attgccgcaa ataaaacaat aacgacagtc
    64861 caaactccaa gattgctctt tttctctttt tttccaattc taaatggtcg attcaacttt
    64921 gcatgtgtat aacgcaatct caaaactgcg atccccatga tgacatagac gatacagtag
    64981 ataacagtag ttgaatttac gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa
    65041 taaaaaagtg caaagattga gataatgaca gactgagtca aaataacgga tttggatacg
    65101 tcaaattgat tcgtcttcca aaaattgaat tttggtggat acgcaccttt tctagcactt
    65161 tctgtcactg tctttgctgg tcctgaggcc caagcagata attggactgc tacaccaatg
    65221 aagacaagta aactaaacag attcacaata agatgtggca aaccaagaat ttggcaataa
    65281 atcgaaatag attgcgcgat attattcaat tccatttgtc cttttggtac gacatttgct
    65341 acaactaaag cattcaaaat attgacgaaa aatataaaaa ttaacgcggc aaatactccg
    65401 cgaatatacg ttttaactgg attttccaat cgagtgatat acacagagga catttcgata
    65461 ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct
    65521 ggtattaatt tgtcccatga aaaactgcct aatgttccgg ttggtacgat cccaactttg
    65581 atccacgcag caaaccctaa taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa
    65641 acaccgactt ttccgccgat ttttgccata tcaaatttca aattcaaaag agtgatgatc
    65701 caataaacga ctaaaatgac aactaatgta aatttgctgt tcaaaccaag cggcacatta
    65761 tcgataatat taccaaacaa cggagcgagt gctgaagcga ccatcaccat cccaggaaac
    65821 atttggaccc ataacagcca tgagacaacg aacccccatt tttttcctag agcattcgat
    65881 acccaaagtt ctggaccacc tttttgggga aacattccag caaattcacc agagatcaat
    65941 gagattggaa aagcgtacaa taatactgca accagcatgt aaaaaatcat cgtccagctc
    66001 gttgctgcta catctggaat atttcgtaca cttgctacaa gggcgcaggt cattccgata
    66061 aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt tcatctttat tccttctttc
    66121 aatagcgaca tatgtttttg ttttctttta tttttgttca agaacagaaa tgacaaggtt
    66181 tcatcgattc agtacaaaac ccagtatcac cgaaacatcc attgaacgat cttagtgaac
    66241 tgggtttgat acatgaaacg aacgatccaa cttatagaac gttttataaa aaagaatatc
    66301 ttggcgagga atatatgaaa aacaggggga aatacaattg ttttgatgaa gcaaataatt
    66361 gtctgttctc catatgtctc ttgttattct ttcacacaaa aatcagttct attggatgat
    66421 ttcgtttagt ttcttttcca ttgctttggc gatttttttt gtataaaaat tgaaaattcg
    66481 atcatcattc aaataaggag tcatgatagc cgcacgtagc agagtgattt tctgttcttt
    66541 tttccattct tctttcgtta gacccattct ttcaacaaaa cgaatcggag aatcaccgta
    66601 atcttcttgt gtaaatgtag tatgagaagt aataaaccgt tcaccataaa catcgccatc
    66661 catataagaa gaccgatcga acattttttc atttaattca ttgatagcgt tcaaatctgt
    66721 acagccttgt tctttgaata cccaattgac catattaaaa tcaggatgat tcaatggata
    66781 gacttcgatt gtttttcctt taacagtaaa agtcagatga tcgagaaatt ctctgaatct
    66841 ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa
    66901 tacacgatgt gctgtccaga ctgcggcggc ggttgctcca gcttttgatc cttccaaaat
    66961 ataagctcct aacatatctg gagcctttac tgatttttca aacacatatg gcgcgaaata
    67021 agaaatgata tttctcatat ttttatgttt gattacgatt cctccagcag cataagggac
    67081 atagcccatc ttatgaggat ctatcgtgac agaatccgct tctgaaatag ctctgaaccc
    67141 ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa
    67201 ttcagctaag gaagcatacg gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc
    67261 atagccgcca tatgcggcat caacatgtaa atagaaatag atcccttcat cttttaacct
    67321 ttctcttaat tggacgattt tatctacact gtctacttga ccttcttctg tcgttcctac
    67381 gacagcaacc acacctagaa ttgggatttt ttgtccagct aattcacgaa ttgttttttc
    67441 caaagcatta atatccattc gataatcctc ttgaactggg atagcaacca tctgatctaa
    67501 gccgactccg caaatatcta acgccttcat ccaagaatag tgttttgttt gagggaccag
    67561 ccatttccct aatctttgga tattttttcc gctacgggaa gaagctgctt tgacctcatc
    67621 catttcttcg tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag ataaattcaa
    67681 aagttcccac tcagacattt ttttcacttt ttcaggcagc acttccttga cagctaaagg
    67741 aatcgatttg atgcaacggg cgtaccataa tccttcaaga ttagcaatag aaccatctgc
    67801 tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc
    67861 tgcttccatt tgcgaagttg ccatagaaga ttccaatgca acattatttg ggttccacag
    67921 catcgcatag ttgtaagcta ataaagcagg catcaatgtt tcagcattca tttgtcccca
    67981 gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac tgacttcatc
    68041 caagacctct atcatatggt cgcgggtggc tagataatct ggagtgagct tatctccttc
    68101 gctaattgca ggcatatctg aaggaatata attttctctc catccaagat gttcatctac
    68161 catcttgttc aacaacattt tataaactgg tccattttca gctttgtcgc ctataaagac
    68221 ggccttgata tccatatctt tcatttttct tccctccata ttttgttggt caatgtcttg
    68281 cagatacatg tgtttatctg taacacctct tcactatagc gccaaaaatg agaaattttt
    68341 ttttataaca tcaaatccac gtagattttt acaaacaaaa ataaataaag tcggattttt
    68401 ctgataattc aacgcaaaaa agcgaacaat ttttcaataa atcagtcttc taaaatagat
    68461 aacctaaaaa aagcgaacaa acaaataaaa tgactgtcct tcattcacta aacatatttt
    68521 tattttttta tattgttaaa aattcacaat ttatttttta atagaattat ctgttttcca
    68581 aattttataa cataaaaaac cttttttaac ggcatttata tgctttttca gaaattaact
    68641 gatgatttca aaaagaaaac atgtcataat atttcccata ttgtgcaaaa aaatacaagg
    68701 cggaggctct actatgattg aagaatatat tgagaaaaat attttaagac agctgttttt
    68761 atgtgggcaa ttctatgtaa ataaagaagt caatctagaa aagctctcca atctcctgca
    68821 tgtttgcaaa accactttac tcaatgatat caataacata aaaaaagaat ttgaagaaca
    68881 gatcgcttat acacatcggg aaaaggaccg ttacacttta tatttttcag aacatatacc
    68941 tcgatgcaag atcatgcagc aattgtctca aaattcgttg tttttaaaaa cttgtctgct
    69001 ttatttggaa gaagatgaac ctgattatct tcaattaact gaatgtgaat tcatctctgt
    69061 cagcaaagct tattcattaa aaaaacaagt attagcttac tttaatgact gcgggatcga
    69121 aatcgaccgc tactcccctc gattcactga aatggaaaga cgtctgctgc ttcttaatgt
    69181 gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc
    69241 cgatcgtttc attgaatccg taacggaaaa tagtggcaga ttttatgata aagaaaataa
    69301 agaaatctta tcgattggtt ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac
    69361 aatagattca aaatttatcg aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc
    69421 agcatgggaa aacactgatt tccaaaccta ttataaaaaa gaagaattcg cctttatttt
    69481 gacactattc aatctttgta attatggttt ccattcttat cagttgattg cggaagactt
    69541 tcaacagcta caccaagtgt ttattgacaa tactcccgaa ataaaagaac tagttgctac
    69601 ctttgaatct cacttcaatc aagaattatt cggaaaccaa ccgttcgaac gtgcgctgat
    69661 tcatctgatg cgttcggctt gggataacta tcaattattc atgccagaaa aattttatct
    69721 tttaaatgag gaacagacaa atttatataa agaagtccag acaatttttt ccagctggtc
    69781 cagccagtta ccttatgatc tacggttgaa tcctaattgc atgcgggctt ttgtgatcga
    69841 attgagcgga attctccgtc tgacaaaaga acatctgact atctatatcg tcacaaattc
    69901 ggatgttcat tatctgattt atcgggaagc cttggaagct gtaactactt tcgattttca
    69961 agttgcacca acaatttatt caagtatatc tgatataaag aagtatgcgc agcagtcctc
    70021 aaaccgggta ttatgtgaac gtacattata tacaccagat gcggtccaat atgaaaatat
    70081 tattcctatt tcgatcaata cgatcgatcg agctatcatt tcagctgtgc aaaacaaata
    70141 aggggctgtg acaaaaatca tgtcacagcc tcttatttgt tgaagatgcc catttctgta
    70201 acaatctctt gatacacgtc aggctcacaa cctcacttat tttggacatc taggggtccg
    70261 atctctttat ttaaaaaata agaaatgatg gttcggataa ctaccaacgc cgctagttgg
    70321 aaaatatcct gcaaggtagg tttgataatc gattcgatga tcccagctga aacaagaatt
    70381 tccaaactta gaagtatata gcctcccaac atctttttaa tagcattgtt ctcgcttact
    70441 cggtcgcttt gcacttttgt atggacaaat aaatatttca aaaaatcctt gattgataga
    70501 caaacacccc aaaccaataa acagatagca aagatatcaa gtcccagtac aagtttttcg
    70561 aaatatggaa gaagaaactg catcaactct gtcgtcatcc tacctctcct cttctttgtt
    70621 tttctttgtc ttctatttcc tgattcaaga aataagaaat gactgttcga atgataataa
    70681 taagtgcaag cttcaaaata tctgtccaag tcggcttgat aactgactcg atgatatcag
    70741 caacgactag tacttccaag cttaacaaaa tataacttgc tagcagtttt cttgtatctg
    70801 cattacgagt attacgttct gcatagtttt tcttgacatg agaccaatca aaaagtttga
    70861 atagaacaag aacgatcccg acaattaaga taccgataga acaaatattt aaaatagcaa
    70921 tgatccattc tagaaattca attaaatttg agcgaaacat atacgctacc tactttcttt
    70981 taatttgttc ctattctttt attgtaaaag aaagttacct tgttcgtcta tttaaaagtt
    71041 gttctaatga taacggttcg cttaaatttt tctttttcac tctcccattt ttctccctcc
    71101 atccataaat cgtggaagta tccaaaacaa cgtagcaagt taaaataagg cttagtccgt
    71161 catcagcttt tcaaagaagc actgtctcgg tgcttttttt gtacccaaat ttctaaaaat
    71221 agaaccatac acctcctcaa atcttttctt cttacctacc ctatttttat ttacaggaaa
    71281 atcagacact tcttcttttt tgtcaaaatt tcctttttct tctcgtccta tcatttggca
    71341 ttctaataaa aacgaactat ccttggatat agaaagagaa aacaaggagg cgtttttcat
    71401 gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg cttgcccttg gcgctgcata
    71461 tctaggaaaa aagacaggac tattcgaaga tgacagtcat ttatatgatg aattcgaatc
    71521 aatctagcat cctatttagt tgaatcacta cggacatact tggaagacat tggaggaatc
    71581 acaatgagaa aaagtaaagc aataatgatt ggtgccggta ttgccaatat ggcagcggct
    71641 gtttatttga ttcaagaagg caaatggaaa ggggatcaga tcacctttta ttcattggat
    71701 gatcacggtt caaatgatgg tgcaccgacc gacactgtag tggatgaata ttggaataaa
    71761 aaccatccat tagaaaacca aaaaggctat attgctcgag gcggtcgcat gcttaattac
    71821 cgtacctatg tagatttgat ggatctgtta agtcgaatac cttctgttac tgagccaggg
    71881 atgactgctg aagaagatac gagagatttt gatgcaaaac accgaacctt tgacaaagca
    71941 cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa aactgggatt caataataaa
    72001 gaccgtattc ttctgactaa actgattgct atgcctgatt cagaagaaga aaaactagac
    72061 aatatcacta tcgaagaata tttcaaagat gatccacact tttttgaaac aaacttctgg
    72121 tttatgtggg aaacaacatt tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc
    72181 tatatgcatc aaatgattta tgaattcact caaatcgaac atctagtggg cgtcaatcga
    72241 acacgttaca atcaatacga aagtattatg cttcctttga tcgagtactt gaaagaacaa
    72301 ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg aattcaaaga aacaccgatg
    72361 caagatgaaa tcactgtgac tggtttgaaa attaccaata cactgactca agaagaagaa
    72421 tacgttgtgg tcgatgaaga tacagcagtt attttcacta atgggtccat tacagattca
    72481 gctactttag gagattacga aacaccagct gtagaaaata tggattatgg tgcagcttct
    72541 agtctctgga aaaaagccag tgaacgtttc tacaacttgg gaaatcctga caaattcttt
    72601 gctgatcgag atgcaagcga atgggtcagc tttaccctga cgacgaaaaa ccatttgttg
    72661 ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa atgccttgaa ttcatttatt
    72721 tcaaccacac cgatcacgcc gttaggccaa aaagatgtca atatgtctat tgtcgtgcat
    72781 catcaaccgc attttacgtc tcagaaacca aatgaaacag ttatttgggg ttatttcctt
    72841 tatccaagac gacgtggcga atttgtggat aaacagtata ttaaaatgaa tggaaaagaa
    72901 atgctagaag aactgattgg acaactttca aaagtagatc ctggaccagt gaatattcgt
    72961 gaaaaagaaa cagaaatttt cgacagcgta ataaataata tccctgtcta tatgccttat
    73021 gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat
    73081 tcgaccaatc tggcctttac aggagaattt gtcgagcagc cttatcagat gatctttact
    73141 gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc attttgcggg tattcctatg
    73201 tcccgtcttg tcaaaacacc gcgttacgac aaagatattc ctactctagc tcgtgccgca
    73261 aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg ccctgcatca agtaataaga
    73321 acaaccaggc taaaacagct tcttatgttt ttgatttggt tggacttatt accgcagatg
    73381 caagcttttg aacaccgttt accaagaggt tatgaaaaag ctgccctgca tcaagtaata
    73441 agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt attaccgcag
    73501 atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta gtttcgagta
    73561 tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga cttaataccg
    73621 agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt tttgaaaaca
    73681 actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga tacaactatt
    73741 tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac aaatacataa
    73801 gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc
    73861 tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat ctgaaaggag
    73921 catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc agaaacgtag
    73981 atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca acagccattt
    74041 gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa gggtatgttg
    74101 taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa acattgaaaa
    74161 aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat ttctgcctga
    74221 atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc gggcttttcg
    74281 gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta agagttcctt
    74341 atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa aaagttttat
    74401 ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga gtaaaaaaag
    74461 gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa aaattcgctg
    74521 ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt ctagataaag
    74581 cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa gctggaacag
    74641 aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta ggaatggacg
    74701 gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc ggcacaatct
    74761 ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa gtaactggtg
    74821 tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg
    74881 tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca atgattgcag
    74941 aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca gaagcagcac
    75001 aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt
    75061 gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga aatacccatg
    75121 agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc cgcttctatt
    75181 tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg attttctata
    75241 tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc tatttttatt
    75301 tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg aacattttta
    75361 tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca tgagaatttt
    75421 tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac gagcggtcgt
    75481 cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt
    75541 atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt ttctgatttc
    75601 cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa acagggcaca
    75661 aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc
    75721 gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt ccttattagc
    75781 gattccagta cgaatacttt cctcatacac aacagcagga actccccatt ttgatgaaaa
    75841 ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc agttatatgt
    75901 aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac aagtctttat
    75961 gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa acggaaaaca atcacttgtt
    76021 ctccgttttt tgattaatca agcatctaaa gcttcttttc ttctttcttg gatcgtagct
    76081 accttgacga acggtagata gatcaaaata gaaagaacca aattgacagc ggctagtaca
    76141 ccacctgcaa tactttttgt tgccaataca ccgccaataa ttggtggagt tacccatgga
    76201 ggcataacgg tagctgctgg cactaaacct gtagaagtcg caaagtaagc aacactaact
    76261 aagaccattg gtgtcaaaat aaatggaatg aacatgatcg ggttcaacac gataggtaac
    76321 ccaaacatcg ttggttcatt aatattgaag acacctggcg caatcgacaa gtttgttaca
    76381 accatatatg gtttgttttt acgtcctact aaataaatag ctaacagtaa acctaaagtt
    76441 gcacctgttc ctcctaagtt gacaaagctg tcaaagaaag gtttgttcac gataaacggt
    76501 gcgctatgtc cagctgcgat tgcattaacg tttgcttcaa tggctggcgc attgattgtt
    76561 tgcatcaaag gatcgaccat attcgctccg tgtaaaccaa agaaccataa gaatggtgtg
    76621 ataaatgcaa gcagtaaggc agatggataa gagtttgcaa gtcccataaa tggttcttgg
    76681 accactgtat agaaagaacc tacgatatca tcaacaccga atgcagcaaa aattgcagca
    76741 actaaagcaa acgcagagat agtgatcatt gctggtagta atgaagcaaa tgcttttgca
    76801 actgccggtg gcacaccatc aggcattttg atgatcaatt tttcatttcc gcttaaacgt
    76861 acaaagattt ctgttgaaat aatcccaaca ataatagcaa taaacaaacc gtttgagctc
    76921 atacctaatg ctccgcctaa agcgaagaaa gagcctaaag aaatcacacc tgcggaaaca
    76981 ccgtcttttc ggtagccgtt agctaaatga taagcaagca agaaagcaat caagacagaa
    77041 agaatggcaa atgtaccgtt ccaagcagca gccccgaatc ctttccatga ttcaccacca
    77101 aaaattgagt tcatcaattc ttggaacccg ggaatcggta agttattgat cattactgct
    77161 aatgctccta agatcattaa cggcatactc accataaatg agtcccgaat cgcaactaaa
    77221 tgccgttgtg caccaatttt tgatgctaca ggtataaaat gtttctccat aaatgcaaca
    77281 aatttatcca taaatcttcc ctcttttctt tactgtagat tacgcgttca atcgacgata
    77341 cagattaatg atctcttttg ctaaatctgt aaaagctata gatgtcatca gatgatcttg
    77401 actgtggacc gtcaaaagcg tgacttcaac gtgatttcct tgtgcttctt gtgtcagcat
    77461 ctctgtttga gagtgatgtg cttggactaa agattcctct gcatctttga ttttttcatc
    77521 tgctaaagtg aaatcacctt ttttcgcagc agcaatcgct tccattgcat cgctttttgc
    77581 atttccgcca aacatgatca gacccatgat ggcttctaga tttttttgat cttccattta
    77641 ttgtatcctc cgataatttt tattatttgt ttattagttt ttctgcttgg tcgagtactt
    77701 tttcaccatt catcatgcca tagtcagcca tattgattac atctaaagga atgcctttag
    77761 gtgctagctt ttcagcaaat tgattttgca taaagcgtac ttgcggtcct aaaagtaaaa
    77821 cgtctacatt tttagattct agttgattat ccgcatctga agcagataca gcaaagatgt
    77881 ctgcttccat tccttttgct tctgctgcct tttgcatttt tgtgactagg agacttgtac
    77941 tcattccagc tgaacataca agcatgattg ttttcttttc cattttaaac acgttccttt
    78001 ctatacttac acttgtatat aagcaagtag cgtgccaact ttaaaagcgc tttaaaaagt
    78061 tgatatcatc acatttaagg tctataccaa gttgcataca cactaaaaaa gaaagcgcac
    78121 actaaacaca caaattaatt tttattacct tttttctttt aacgtgcaca aaaaaagttg
    78181 tgacacaaaa cttgtcacaa cttttttcaa ataaacggta ttcaaaagct tgcatctgcg
    78241 gtaataagtc caaccaagtt aaaaacataa gatgttgttt ttactttgct attcttatta
    78301 cttgatgcag gacagctttt tcacaacctc tcaataaacg gtgttcaatc agctgaccct
    78361 gggtattagc tcaaaacgct ggattcacaa cctcttttat ttcaaataac cagtaaatac
    78421 aatgaacatc tacaaaaaac ttctatttat ttatagattt cgtcaggcaa atccaattct
    78481 ctcttatcaa tcaaagtctg ataccacgaa gcagattttt tcaatgttct gcttcgttca
    78541 tcgttcaatt cgattcgaac taatccatat cgattcttaa atgcattcat tggtgacaca
    78601 cagtcagtga atgcccataa catatagcct tcgcaatttg cgccttcttc tgttgctttg
    78661 atcaagtaat gcaaatgctg tccgataaat tcgatacggt aatcatcttg cacgattcct
    78721 ttctcatcca tatactgttc ttcatattct cttcccatac cgttttctgt tatcagccaa
    78781 gggatattat gataattttc ttttagatac atggctatat catacatgat tttcggatag
    78841 atttcccatc ccctggaatt attcattttc ttcccaggta aatcaaagac ttcataatat
    78901 cgttcaggat ggaaaggtgt ctcttcattc cattgataac gaggggcgcg aacgcgttta
    78961 ggataatatt gattgattcc aaggaaatct actgtatgat gctgaatcgt ttctaaatcc
    79021 tctgttgcag gatcgaaata aatcgattgt ttttgacaca actccaggaa ctcttctgtg
    79081 tagtatcctt ttaccatcgg atcaaaaaat actcgattat aaaacaaatc atacatataa
    79141 gcagcttttt ggtcttctgg agaattggat cgagcataga ccatttcagg atttaaaata
    79201 cagccaatcc ttccaccata atttccgctg cggtaaatct ctactgcttt agccgttgct
    79261 aaaactttat gataattcca catcatccac ttttttgtgt tttgttcatg cggccagcga
    79321 acagcatcca aatagcaacg cgtttgggga acgattggtt cgttgaatgt aaaccagttt
    79381 tttaccctat caccgtaacg atcaaaggca atttgagcat acttagcata aaaatcaact
    79441 acttttcttg aagaccagcc atcatatttt tccaaaagaa aagctgggac ttcataatgt
    79501 tccaaacaaa tcatcggttc tacgtctgct tcgatcaaag agtcgatcat ttcacttata
    79561 tgctgagcat attcttcatc tactatacag ttctcataat cagtaaaaaa acgagcccaa
    79621 ttgatagaag tccgataatg tgtcagatgg atggatttca tcagatccac atcctgctgg
    79681 tattgttcca taaaattagt tgctacggct gggccatacc cattatgcca aacaaaatgt
    79741 tcatttttat accagctatc tataaacgaa tcctgtcctt ctttttttcc tttccaacct
    79801 tccgtttgcc aagcagaaga tgcagcacca agaataaaat tttttggaat agtaattttc
    79861 atgatttcct cctgtttttt agatcccatc attttccaca cgatttgcta cttttacaaa
    79921 tggcagatag atcaaaatag aaacaataat acaaacaagt tgcgtaataa ctgcaccgat
    79981 atttcctgct gttgccagat aagcattgac gatcggcggc attggccaag ggaccattac
    80041 aactgctttc gccgcaaatc ctaaagcggt tgcaaaataa ccaatcgttc cagtgataag
    80101 aggcgttaag ataaacggaa tagctaaaat tggattcagc atgatcggca taccaaaaat
    80161 aactggttca ttgatgttga agatcccagg agcaagggat aacttggtga tttgtttcat
    80221 atcttcccgt tttccaacta acaatactgc aatcagtaaa ccgatcgtga ctccagaccc
    80281 gcccatactc atatacatat cccaaaatgg catagtaata atgttcggta cttcttttcc
    80341 agcttcaaaa gcttccgtat ttacagcaat tgccgctaaa agcaacggtt cccgtatcgg
    80401 cttcaccatt tgatttccat ggataccaat cacccaaaag acttgcgcaa taaacatcaa
    80461 tagcaaaata cccggtaacc cttgtacgac accttctaat gggcgttgta cgacattgta
    80521 aatgatatca taaaggtaca ttcctgtaag ttctttgatg ataaatcccg cagccgcgat
    80581 acccgataca gtcagaatcg tcgggaataa agcagaaaaa ctagcagaaa cattcgttgg
    80641 cacggtatca ggcattctca gctgtagttt cttctgtttc ctcaaccagc aataaagttc
    80701 aatagaaagg atggagacca acattcccaa aaataaccct ttcgtatctg tatattgttt
    80761 agccaatacg ttttctacta taacagtctg attttcattc actaattcat aaacagttgg
    80821 attaacagat aaataagaca tcactgcgat gataccaggg aatacaccat ccactttatt
    80881 cagcttggct aattcgatcc ctatcagaaa aacagcatag atggtcaaga aacttagtgt
    80941 gacatagctg atggcagatg cgataggctt taattcttct aaaaaacgca agaaacttat
    81001 ctgtgctaag ccattttccg agtcaaagac cattgctgaa aacaatgtgc caaaagcacc
    81061 cgtaataatt accggaatca atgcagcaaa tgcgtttttt atcaccatga tataccttaa
    81121 attattgatt ctcgttgcca cgttccctaa aacattggct aatttatcca taaaactcat
    81181 tccagatccc ctcattcctg cttcttctcc ttgacgtcat ctctttttgt ctattagtca
    81241 gcgcttacat ttcgatgaac agaaactatt tactcctctt ttttaccaaa tataaaacaa
    81301 aaattcatct gatgtattta tcataaaata catcagatga actgtcaatt agtttctttt
    81361 tctcctatta ttttctgtaa ttctctccga tttattgcca tatggacagt cattgcatca
    81421 aacgcagcaa gtgcattccg cctttcgatg gcctgaacga tctctgtatg aatatcgatc
    81481 gtctcgattt tcagatgctg gatatcataa tttttattaa acaaagcaat tgattcatta
    81541 atgattggga taatatgcag caaggctacg tttccgctag cttctgcaat catcgtatga
    81601 aaatcgatgt ctaattgaac atgttcttgt gtgccttctc gaaaagaagt ttcaatcttt
    81661 tgcgttaatt ttttcatttg gacgatttga ttcttcgttg catattccgc tgccaaacct
    81721 gccatcttag gctccaataa atatctcagt tcaaataagt ctcgtatcat ccgatcctga
    81781 tcttcaatca aagaaaaacc gagtgggtca ttggcaatcc ctcgcttatc actgacaaat
    81841 gtccccgctc cttgccgaac ttcaaggata ttcctggaag ctagagcccg cacagcttct
    81901 cgcaccgtgc ttcgccccac ctctaaataa tcggacaatt catattcatt cggcagttta
    81961 tctcctactt gcaactgcat ttcctttaaa tatctaagga tcttgtcagt agttacttct
    82021 actagggttt tattttcttc caaaccacat acctcctttt catctgatgt attttttcgt
    82081 aattattata acacgtttga atcatctgat gattttttgt atacaaaatt gattaatccg
    82141 ttcatagaca tacaaacgaa gaagatattt accataaaaa agactaggat aaaattcact
    82201 cctagccttc gatcattttt attttgaacg acaaaaaata tgatgttcaa taacagacga
    82261 tcttcattcg attcttgatg tcatcttttg ttttttttaa tatatggata aaaagtttga
    82321 ttacatcgtt tctattctag aatttggtcg attctcaatt cttcttctgg tgagaaatct
    82381 aaattatcca tcattcgaac actttcttgt aactgactta accgacttgc tccgaccagt
    82441 acacttgtaa cagatttttg tcgcaaattc catgcaaggg ccatttgcgc taatgattgt
    82501 cctcgtgata cggcgattgt ttgcagtgct ttgatttttt ccagagttga tccaacttgt
    82561 tcttctgaaa ggaatggtat ttccttacga tgtgctctag aatcttcagg aataccatgt
    82621 agataacgat ttgtcagcaa cccttgagca agcggactga acgtgattgc ccctagccct
    82681 tcttcttcta atactggaga tagatcgtct tctagtagtg cacgagaaaa catattgtag
    82741 cgcatctgat gaataatgaa cggtgcttcc tttcttttta aaatttcagt catttttttt
    82801 gtatcttctc cattatagtt ggatatacca atatataatg cttttccttg tcttacaagt
    82861 tgcatcaaag cttctgctgt ttcttccaaa ggtgtatcag ggtctgggcg atgatgatag
    82921 aagatgtcaa cataatctag ccccattctt tggagacttt ggtcacaact tgaaatgatg
    82981 gatttacgag agccccattc cccatatggc ccttcccaca tatgataacc tgctttgcta
    83041 gaaataatca gttcatcacg gtaggcttga aaatcttctc taaaaatacg cccaaagttt
    83101 ttctctgcag ctccagcagg gcctccataa ttattcgcaa ggtcgaaatg ggtaattccc
    83161 atgtcgaatg cgcctcttaa aatctctctt tgattatgga taggatcata atcaccaaaa
    83221 ttttgccata atccgagaga taaaatcggt aatttcaaac ctgaatgacc ggatttacga
    83281 tattccattt tttcataacg tgcactatct gcaatataca atttttctcc tcctcatcaa
    83341 atctttttaa aacgctttct tttttctatt tttagtttac tacattttaa agaaaaggag
    83401 ttaatcaaaa tgattttttc gtatattttt ttgccatttg ttttttactc gttgatagat
    83461 cagaaattga ttacgtgctt gctggatttt tttataacgt ttcggttgtt ttttgtaaaa
    83521 ttgctgatga tagttttctg ctggccaaaa cgtaccggct ggttcgattt ttgtgacgat
    83581 tggttggtgg aaagttcctg aactgtctag tttctgtttt gattgttccg ctaattcttt
    83641 ttgtcgttcg acttgataaa aaatcactgg acgatattgc ttccctctat cttgaaattg
    83701 tccaaatgta tctgtgggat cagtaatttg ccaatagatt gtcagcaact cgctataact
    83761 tatttctctt gtgtcataaa tgatctccac agcttccaca tgacctgtgt aaccaccact
    83821 tacttgatca taatttggtt tttcaacatg tccgcccgtg tatcctgaca aaacagatag
    83881 gatccctttt ttcgattcaa acggctcgac catacaccag aaacatcccc ctgcaaatac
    83941 tgcacgttct tgatagtcag catcttgcat ggcatgcttg gaaaagtcat atttttgctc
    84001 cttatcaaat ccttccgtaa tcaataaata aaagtccatt acatcaggcg tcagattcat
    84061 tcggatagct aatggacgta gtgttgcttc tagttttgat aactgttctt taacggatat
    84121 attttctctt atgtcattct ttgtctgaat caatactttt cgttcccaat ctcgtgtatt
    84181 cggattcaaa atcagattat aaagtgtttg aatgaatatt tgttcctcat ccatagattc
    84241 tcctcctttc cgaagaagac aagctagtga ctttagctgt aatttatcat aaagtgagga
    84301 aataagcgat tacttcatcc ccctacagat ttttcgtata aaaaagagag aatccttatc
    84361 gacagattct cctttttaat caatttattt tataaatgaa aagttaattt accttttatg
    84421 gaaaactatt ttaaaaaata aacataaata tgcagttaat gtaacaagat aagaaataat
    84481 agtcgttact gtataacttg tgcttacttt aatatcagca actcctttga ctccgcttat
    84541 ttgtaccaat ccattagcat tcacctgctc ctttacaggt tgatgattta tttctacatt
    84601 cgttcctaaa taagcatata caggaagtgt gattacttgg ctcttctcat tagttactct
    84661 atatatgata gaactaccag ttgttttata aggaaggttt acccattgac tgccaatctt
    84721 tgcttcgtgt tttattatat gctccttatt ctccaatgaa gcgataggag aataatcata
    84781 taaaaaataa tccattcctt tagtggtata cgcctctact gtttctttgt ttacaacaat
    84841 atgtcccggc tgagaaatta ttttttgatt gaaatttttt ttactagaaa cagttaaagt
    84901 gattaataac aaaacagtca taaataaata aaatagttct cttgtcttat tgtatcttct
    84961 atttttgaaa aatatcatcg ttaatatcag agaagagaat attatttgca agcttaagat
    85021 ccgccaagga aattgtaaca atttggctgg cgtattttgc agaagatacc atggcaccaa
    85081 actagttgta gctagccaag tcaaacacgt accataataa atcttttttt ctatgtttgt
    85141 taatttttta aataaaatca ggggaataca taaagataac agaccaacta gtcccattga
    85201 gtaagtagtt aagtcgttgt tcatagcttc agtcagacta tcgaacacat tagatgcatg
    85261 agcatataaa tttgtcacgc taggtttgtt gattttttgg tagagcattt gttcaaacat
    85321 cggataccaa aaagcactcg tcagaaccaa tgtcattcct gccgcactaa ataatttttt
    85381 aaatatagca ataaaatctg cattcttgaa acctttagaa accaaacgaa taaaaataaa
    85441 aatgataatt aatgctgtac tcattaatgt tgatagtaca tggctgtaca ctagcaaact
    85501 catactcaat gctagcttta cagacggata cttttcttct tttccagaga tgatttgata
    85561 agcataaaat aatacaggga taacaagtgt tattgcgata gcttcggcaa ttgcggaacg
    85621 atagtagatg tctactgtcc gataagcaga gaaaatatag agacaactaa ataaaaatgc
    85681 cgcttttttt tgttttaaaa accgctcgcc ataatgataa gctacgagtg ttgtacacaa
    85741 gttcaataac caaacgtaaa taatatagct tacaatcaaa ttttttgtca tccaatataa
    85801 cattacagct ggaaaaaaag tcagaaaggg ataaaaataa tttacaccat atccagtata
    85861 attgaatgtc tcataattaa tcggactggt aaaaatagtc gataacccct tgattcgaga
    85921 taagtgaaaa gaaagatctc cagtggtata aatactctgt gtatttttga aaaagatgaa
    85981 agaatatata ctactaacta taattaatcc actcaatatt actacttttt tatttatttt
    86041 cattcttctt gtttatctcc tttttagtca catatcgaga atctttcaac ttattaggtg
    86101 caaatcgttt aaaacttgaa tacaccacta aacaataagt cagaatactg atccataaag
    86161 ccatgagagc agttggagta tattttgttg taatttctat cttattttct ccagcattta
    86221 catccacatt cactaagcca tgttttttat aagagtcatt tacgacttta ccgtttactt
    86281 ttacctgtgt acctaaataa gcatatactg gtactgtcac tttttgcttt ttgttgcttg
    86341 ccactgtgta agtaatgtta ttatctgcag ctttatactt ccctttttcc cacgattctc
    86401 ctacttttac ttcatgtttt tctaagtgag gcctttcttt tatcgcttct gttggggcgt
    86461 aatcgtataa tcctcccata tctgatgttg tataacgtgg gagtgtttct tcattgataa
    86521 caatatgacc gtgttttgat tggataactt ttgcgtaatt ttcttttgct gctatagacg
    86581 tgacgagtag aaacaccaca gtagcgccaa tgattaacca tgaatatttt ttattcatag
    86641 tctttttcat aaaaacaagc gttatgatca ttgaagaaaa aaagatttgg atacctaaga
    86701 tacgccaagg aaactggagt aagcttatcg gtgttttttg aaacaaaaac catggaaata
    86761 aattagttac gcatacccat gtagaaacta cgccaacata gatgattttt tcttttttct
    86821 ccatttcttt cagtatgaat aaaggaaaaa tcaacgcaac caatcctaat aaccccatag
    86881 tgtatgttgt aatatcgtta tttaacgctt gaatgatact atctgaaata gaaagtgctt
    86941 ctagctgcaa atctgttttg aagggactgt gtatttcttg ataattcatc tgctgcaaca
    87001 taggatacca aaaatagcta gttaatgcac aagtcaaaaa aacagccttt actaaattca
    87061 cgaccacaaa ttttatatcg ttttttattt gcttgcttaa aaacaaacga actataatca
    87121 tacaagcaat caatgcacta cacaatagtg tagttaagac atgagtatac acaagtagac
    87181 ttatactgac agataaagcg atgacttttt tttctctatt gaatatcaat ccataagcat
    87241 aatataaaac agctgggata atcgtaagag cgattgcttc tgcaattgcg gatcgataat
    87301 agatatcaac tgttcgataa gttgcaaaag tatataaaca actaaataaa aaagcagcat
    87361 ctatcttttt aaaaaacttt tctccataat aaaaactaat cagaatcgta caaat
//
LOCUS       DAJSBC010000003        83038 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSBC010000003 DAJSBC010000000
VERSION     DAJSBC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.003"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..465
                     /locus_tag="ONT65_000162"
     CDS             25..465
                     /locus_tag="ONT65_000162"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2327824.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            593..1276
                     /locus_tag="ONT65_000163"
     CDS             593..1276
                     /locus_tag="ONT65_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR2327825.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1585..1658
                     /locus_tag="ONT65_000164"
     tRNA            1585..1658
                     /locus_tag="ONT65_000164"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1619..1621,aa:Arg,seq:tct)
     gene            1817..2176
                     /locus_tag="ONT65_000165"
     CDS             1817..2176
                     /locus_tag="ONT65_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR2327826.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2299..3930
                     /locus_tag="ONT65_000166"
     CDS             2299..3930
                     /locus_tag="ONT65_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR2327827.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3992..4633)
                     /locus_tag="ONT65_000167"
     CDS             complement(3992..4633)
                     /locus_tag="ONT65_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2327828.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4819..4895
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4915..5223
                     /gene="rplU"
                     /locus_tag="ONT65_000168"
     CDS             4915..5223
                     /gene="rplU"
                     /locus_tag="ONT65_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR2327829.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5250..5594
                     /locus_tag="ONT65_000169"
     CDS             5250..5594
                     /locus_tag="ONT65_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR2327830.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5607..5900
                     /gene="rpmA"
                     /locus_tag="ONT65_000170"
     CDS             5607..5900
                     /gene="rpmA"
                     /locus_tag="ONT65_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR2327831.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6334..9141
                     /locus_tag="ONT65_000171"
     CDS             6334..9141
                     /locus_tag="ONT65_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2327832.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9332..9823
                     /locus_tag="ONT65_000172"
     CDS             9332..9823
                     /locus_tag="ONT65_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2327833.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9926..10915
                     /gene="manX"
                     /locus_tag="ONT65_000173"
     CDS             9926..10915
                     /gene="manX"
                     /locus_tag="ONT65_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR2327834.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10948..11751
                     /locus_tag="ONT65_000174"
     CDS             10948..11751
                     /locus_tag="ONT65_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR2327835.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11772..12686
                     /locus_tag="ONT65_000175"
     CDS             11772..12686
                     /locus_tag="ONT65_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR2327836.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12769..13143
                     /locus_tag="ONT65_000176"
     CDS             12769..13143
                     /locus_tag="ONT65_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR2327837.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13282..13527
                     /locus_tag="ONT65_000177"
     CDS             13282..13527
                     /locus_tag="ONT65_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2327838.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13699..14763
                     /locus_tag="ONT65_000178"
     CDS             13699..14763
                     /locus_tag="ONT65_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2327839.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15009..15434
                     /locus_tag="ONT65_000179"
     CDS             15009..15434
                     /locus_tag="ONT65_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2327840.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15427..15876
                     /gene="nusB"
                     /locus_tag="ONT65_000180"
     CDS             15427..15876
                     /gene="nusB"
                     /locus_tag="ONT65_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR2327841.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15973..16827
                     /locus_tag="ONT65_000181"
     CDS             15973..16827
                     /locus_tag="ONT65_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR2327842.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16828..18165
                     /locus_tag="ONT65_000182"
     CDS             16828..18165
                     /locus_tag="ONT65_000182"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR2327843.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18170..18394
                     /locus_tag="ONT65_000183"
     CDS             18170..18394
                     /locus_tag="ONT65_000183"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR2327844.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18396..19286
                     /locus_tag="ONT65_000184"
     CDS             18396..19286
                     /locus_tag="ONT65_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2327845.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19295..20113
                     /locus_tag="ONT65_000185"
     CDS             19295..20113
                     /locus_tag="ONT65_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR2327846.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20220..20669
                     /locus_tag="ONT65_000186"
     CDS             20220..20669
                     /locus_tag="ONT65_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2327847.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20687..22369
                     /gene="recN"
                     /locus_tag="ONT65_000187"
     CDS             20687..22369
                     /gene="recN"
                     /locus_tag="ONT65_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR2327848.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22424..22540)
                     /locus_tag="ONT65_000188"
     CDS             complement(22424..22540)
                     /locus_tag="ONT65_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR2327849.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22613..23569)
                     /locus_tag="ONT65_000189"
     CDS             complement(22613..23569)
                     /locus_tag="ONT65_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2327850.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23740..24108
                     /locus_tag="ONT65_000190"
     CDS             23740..24108
                     /locus_tag="ONT65_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR2327851.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24225..24367
                     /locus_tag="ONT65_000191"
                     /pseudo
     CDS             24225..24367
                     /locus_tag="ONT65_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719784.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
     gene            24391..24822
                     /gene="mraZ"
                     /locus_tag="ONT65_000192"
     CDS             24391..24822
                     /gene="mraZ"
                     /locus_tag="ONT65_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR2327852.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24843..25802
                     /gene="rsmH"
                     /locus_tag="ONT65_000193"
     CDS             24843..25802
                     /gene="rsmH"
                     /locus_tag="ONT65_000193"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR2327853.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25807..26208
                     /gene="ftsL"
                     /locus_tag="ONT65_000194"
     CDS             25807..26208
                     /gene="ftsL"
                     /locus_tag="ONT65_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR2327854.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26209..28401
                     /locus_tag="ONT65_000195"
     CDS             26209..28401
                     /locus_tag="ONT65_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2327855.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28427..29389
                     /locus_tag="ONT65_000196"
     CDS             28427..29389
                     /locus_tag="ONT65_000196"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR2327856.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29403..30764
                     /locus_tag="ONT65_000197"
     CDS             29403..30764
                     /locus_tag="ONT65_000197"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR2327857.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30790..31878
                     /gene="murG"
                     /locus_tag="ONT65_000198"
     CDS             30790..31878
                     /gene="murG"
                     /locus_tag="ONT65_000198"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR2327858.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31893..33113
                     /locus_tag="ONT65_000199"
     CDS             31893..33113
                     /locus_tag="ONT65_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR2327859.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33269..34594
                     /gene="ftsA"
                     /locus_tag="ONT65_000200"
     CDS             33269..34594
                     /gene="ftsA"
                     /locus_tag="ONT65_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR2327860.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34617..35858
                     /gene="ftsZ"
                     /locus_tag="ONT65_000201"
     CDS             34617..35858
                     /gene="ftsZ"
                     /locus_tag="ONT65_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR2327861.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35875..36552
                     /locus_tag="ONT65_000202"
     CDS             35875..36552
                     /locus_tag="ONT65_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR2327862.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36566..37165
                     /locus_tag="ONT65_000203"
     CDS             36566..37165
                     /locus_tag="ONT65_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR2327863.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37180..37467
                     /locus_tag="ONT65_000204"
     CDS             37180..37467
                     /locus_tag="ONT65_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR2327864.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37516..38298
                     /locus_tag="ONT65_000205"
     CDS             37516..38298
                     /locus_tag="ONT65_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR2327865.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38319..39026
                     /locus_tag="ONT65_000206"
     CDS             38319..39026
                     /locus_tag="ONT65_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR2327866.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39288..42086
                     /gene="ileS"
                     /locus_tag="ONT65_000207"
     CDS             39288..42086
                     /gene="ileS"
                     /locus_tag="ONT65_000207"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR2327867.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42135..43661)
                     /locus_tag="ONT65_000208"
     CDS             complement(42135..43661)
                     /locus_tag="ONT65_000208"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR2327868.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43676..44323)
                     /locus_tag="ONT65_000209"
     CDS             complement(43676..44323)
                     /locus_tag="ONT65_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR2327869.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44507..44836)
                     /locus_tag="ONT65_000210"
     CDS             complement(44507..44836)
                     /locus_tag="ONT65_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR2327870.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45013..45741
                     /gene="sfsA"
                     /locus_tag="ONT65_000211"
     CDS             45013..45741
                     /gene="sfsA"
                     /locus_tag="ONT65_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR2327871.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45757..46770
                     /locus_tag="ONT65_000212"
     CDS             45757..46770
                     /locus_tag="ONT65_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2327872.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46770..48047
                     /locus_tag="ONT65_000213"
     CDS             46770..48047
                     /locus_tag="ONT65_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2327873.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48110..50812
                     /locus_tag="ONT65_000214"
     CDS             48110..50812
                     /locus_tag="ONT65_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2327874.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50964..51281
                     /locus_tag="ONT65_000215"
     CDS             50964..51281
                     /locus_tag="ONT65_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2327875.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51311..51631
                     /locus_tag="ONT65_000216"
     CDS             51311..51631
                     /locus_tag="ONT65_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2327876.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51739..53199
                     /locus_tag="ONT65_000217"
     CDS             51739..53199
                     /locus_tag="ONT65_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2327877.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53267..53488
                     /locus_tag="ONT65_000218"
     CDS             53267..53488
                     /locus_tag="ONT65_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327878.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53519..53701
                     /locus_tag="ONT65_000219"
     CDS             53519..53701
                     /locus_tag="ONT65_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR2327879.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53701..54114
                     /locus_tag="ONT65_000220"
     CDS             53701..54114
                     /locus_tag="ONT65_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR2327880.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54237..55418
                     /locus_tag="ONT65_000221"
     CDS             54237..55418
                     /locus_tag="ONT65_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2327881.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55489..55653)
                     /locus_tag="ONT65_000222"
     CDS             complement(55489..55653)
                     /locus_tag="ONT65_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327882.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55807..55880
                     /locus_tag="ONT65_000223"
     tRNA            55807..55880
                     /locus_tag="ONT65_000223"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55841..55843,aa:Arg,seq:cct)
     gene            complement(55917..56117)
                     /locus_tag="ONT65_000224"
     CDS             complement(55917..56117)
                     /locus_tag="ONT65_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2327883.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56422..57654)
                     /locus_tag="ONT65_000225"
     CDS             complement(56422..57654)
                     /locus_tag="ONT65_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR2327884.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57908..58477
                     /locus_tag="ONT65_000226"
     CDS             57908..58477
                     /locus_tag="ONT65_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2327885.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58655..59095)
                     /locus_tag="ONT65_000227"
     CDS             complement(58655..59095)
                     /locus_tag="ONT65_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR2327886.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59253..60017
                     /gene="map"
                     /locus_tag="ONT65_000228"
     CDS             59253..60017
                     /gene="map"
                     /locus_tag="ONT65_000228"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR2327887.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60049..60972
                     /locus_tag="ONT65_000229"
     CDS             60049..60972
                     /locus_tag="ONT65_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR2327888.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61048..62187
                     /locus_tag="ONT65_000230"
     CDS             61048..62187
                     /locus_tag="ONT65_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR2327889.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62180..62980
                     /locus_tag="ONT65_000231"
     CDS             62180..62980
                     /locus_tag="ONT65_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2327890.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62980..63807
                     /locus_tag="ONT65_000232"
     CDS             62980..63807
                     /locus_tag="ONT65_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2327891.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63785..64519
                     /locus_tag="ONT65_000233"
     CDS             63785..64519
                     /locus_tag="ONT65_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2327892.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64619..65485
                     /gene="rfbA"
                     /locus_tag="ONT65_000234"
     CDS             64619..65485
                     /gene="rfbA"
                     /locus_tag="ONT65_000234"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR2327893.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65499..66071
                     /gene="rfbC"
                     /locus_tag="ONT65_000235"
     CDS             65499..66071
                     /gene="rfbC"
                     /locus_tag="ONT65_000235"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR2327894.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66093..67121
                     /gene="rfbB"
                     /locus_tag="ONT65_000236"
     CDS             66093..67121
                     /gene="rfbB"
                     /locus_tag="ONT65_000236"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR2327895.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67219..68070
                     /gene="rfbD"
                     /locus_tag="ONT65_000237"
     CDS             67219..68070
                     /gene="rfbD"
                     /locus_tag="ONT65_000237"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR2327896.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68105..70138
                     /locus_tag="ONT65_000238"
     CDS             68105..70138
                     /locus_tag="ONT65_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327897.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70182..71462
                     /locus_tag="ONT65_000239"
     CDS             70182..71462
                     /locus_tag="ONT65_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR2327898.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71672..72478
                     /locus_tag="ONT65_000240"
     CDS             71672..72478
                     /locus_tag="ONT65_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2327899.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72490..73710
                     /locus_tag="ONT65_000241"
     CDS             72490..73710
                     /locus_tag="ONT65_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2327900.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73700..75286
                     /locus_tag="ONT65_000242"
     CDS             73700..75286
                     /locus_tag="ONT65_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2327901.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75325..77463
                     /locus_tag="ONT65_000243"
     CDS             75325..77463
                     /locus_tag="ONT65_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2327902.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77831..78814)
                     /locus_tag="ONT65_000244"
     CDS             complement(77831..78814)
                     /locus_tag="ONT65_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR2327903.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79192..80583
                     /locus_tag="ONT65_000245"
     CDS             79192..80583
                     /locus_tag="ONT65_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2327904.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80599..81639
                     /locus_tag="ONT65_000246"
     CDS             80599..81639
                     /locus_tag="ONT65_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2327905.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81658..82743
                     /locus_tag="ONT65_000247"
     CDS             81658..82743
                     /locus_tag="ONT65_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2327906.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82776..>83038
                     /locus_tag="ONT65_000248"
     CDS             82776..>83038
                     /locus_tag="ONT65_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2327907.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKY"
BASE COUNT        27158 a        13967 c        17583 g        24330 t
ORIGIN      
        1 ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt
       61 attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg
      121 aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa
      181 aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa
      241 gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt
      301 cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt
      361 caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt
      421 gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat
      481 aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc
      541 agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa
      601 tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca
      661 aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc
      721 agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga
      781 acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat
      841 cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa
      901 aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc
      961 agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc
     1021 aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat
     1081 ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg
     1141 caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga
     1201 tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga
     1261 aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc
     1321 tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa
     1381 tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta
     1441 gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa
     1501 ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta attatctata
     1561 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
     1621 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
     1681 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
     1741 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
     1801 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
     1861 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
     1921 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
     1981 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
     2041 atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag
     2101 tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct
     2161 ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa
     2221 aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat
     2281 gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca
     2341 ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta
     2401 gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg
     2461 attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata
     2521 tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg
     2581 caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc
     2641 cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt
     2701 attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt
     2761 acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc
     2821 tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa aaaatgggcg
     2881 aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc
     2941 catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg
     3001 aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt
     3061 gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat
     3121 aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct
     3181 ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct
     3241 ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt
     3301 gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt tttgattgtg
     3361 acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg
     3421 tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt
     3481 gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc
     3541 acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg
     3601 aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc
     3661 aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta
     3721 ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa
     3781 agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac
     3841 ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta
     3901 catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa
     3961 actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta
     4021 cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat
     4081 gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg
     4141 ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt
     4201 tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct
     4261 tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc
     4321 gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat
     4381 acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag
     4441 aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct
     4501 ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag
     4561 cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt
     4621 ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat
     4681 gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt
     4741 tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta
     4801 ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata
     4861 tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac
     4921 gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt
     4981 gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc
     5041 ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt
     5101 gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca
     5161 caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct
     5221 taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg
     5281 caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg
     5341 atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat
     5401 tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg
     5461 aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc
     5521 ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa
     5581 ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc
     5641 acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg
     5701 ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta
     5761 caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag
     5821 ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc
     5881 cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca agacttttgt
     5941 cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag tttactttgt
     6001 ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat aactatcgtt
     6061 ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg taaacagtgt
     6121 tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt taaattgaac
     6181 atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag
     6241 aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag aaaaggagcg
     6301 ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat
     6361 ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga agtgttaggg
     6421 attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg aaaactgata
     6481 aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca cgtaaagcca
     6541 gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa aaatacactc
     6601 caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag catgaaaaat
     6661 tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa ttgtttgatt
     6721 acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca ttttgcaaaa
     6781 gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc
     6841 aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc ttttacaggc
     6901 gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct gtttttggat
     6961 gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat ggatcatgga
     7021 acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt
     7081 gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg tatcccaatc
     7141 aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa
     7201 atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac agaagatgta
     7261 gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa gtcaaatatc
     7321 cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat ctcgatcaca
     7381 atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag caaccgcagt
     7441 gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc aaatgatacg
     7501 atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga tatcatcgga
     7561 gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa tcatttcatt
     7621 tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt ttcttttaat
     7681 gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac caatcagatt
     7741 tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat ttatgctatg
     7801 agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg gcatacgaat
     7861 gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc gaaagaagtg
     7921 cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga ctattacttg
     7981 accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt
     8041 gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca gttgttagat
     8101 gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac agcattggac
     8161 cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact cttagtagat
     8221 atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat tccagtacgt
     8281 actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt ctcagtatta
     8341 gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata
     8401 tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc tgttttggct
     8461 atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga acgagcggta
     8521 aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga agtgaaagaa
     8581 gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat tactgatttg
     8641 aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat cgaagtcatt
     8701 ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc cctagatgaa
     8761 gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt tattaatggt
     8821 agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac gacgggaatc
     8881 ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg aatgatcgaa
     8941 cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa tacaacgaat
     9001 gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca gataaaagta
     9061 aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca gttagataaa
     9121 aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg tatcgttttt
     9181 gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt
     9241 tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat aggggtttct
     9301 gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac gtatagatga
     9361 tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat
     9421 cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac taaaagaagc
     9481 tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc aaatctatca
     9541 tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc aagatgttga
     9601 agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta tgagttttac
     9661 tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata ttgattcctt
     9721 tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg ctgacaggaa
     9781 aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga
     9841 aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga atttgttaca
     9901 acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc atggacaatt
     9961 tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa acgtcaaagc
    10021 cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg aagaagcggt
    10081 tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg gcggaacacc
    10141 gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg
    10201 tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg aatctgctca
    10261 tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga
    10321 attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta aaggagcact
    10381 tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac gtgttgactc
    10441 acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat
    10501 tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa tcgaacaagc
    10561 tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg aagtagcaaa
    10621 agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg aagacgtatt
    10681 gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa tggcgcattc
    10741 ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg ttgaagcgtt
    10801 tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg
    10861 cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat
    10921 aatattaaaa tttataggag gcttatcatg tctattattt caataatttt agtcgtatta
    10981 gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca tcaaccacta
    11041 gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat cgtacttggc
    11101 ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt agcaccagat
    11161 gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca aggtgttaaa
    11221 ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt gttcttaaca
    11281 atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc tgctgaagaa
    11341 ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca aggggttcgt
    11401 atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca atcattcctt
    11461 gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat ggtcgtagct
    11521 gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc attcttcgtt
    11581 attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg tgctttaggt
    11641 gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc aaataatgga
    11701 ggcggaagca actctggtga ccctttaggc gacattttga atgactatta atcttgaagg
    11761 aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg
    11821 cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa aacggtggct
    11881 gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg
    11941 cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct tctccaattt
    12001 taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag
    12061 cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt gacccagtgt
    12121 tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct atgggaggaa
    12181 atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg gcattcatgt
    12241 ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac ctttctggcg
    12301 gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta ttagctgccc
    12361 ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta aaattagata
    12421 aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc aagagtgcat
    12481 ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca
    12541 acttagacca attgattcct ggtttagctg cattaggatt aacattgtta tgtatgtggt
    12601 tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta gtcggtgttg
    12661 ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt tgtcccgagc
    12721 tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat ggttcaatca
    12781 atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct gacagattac
    12841 gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga tagacgcaaa
    12901 tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat gctaaaggga
    12961 aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac attttcttcc
    13021 aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga tagaatggta
    13081 caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag
    13141 taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt tgcagaagaa
    13201 aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct aaaattgatt
    13261 agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg agccgtgatc
    13321 ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt aacgaatatc
    13381 attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg gggaccaaaa
    13441 gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt agcagttgtc
    13501 tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa aaggagttgc
    13561 agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag ttttccggaa
    13621 atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa
    13681 gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa
    13741 gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt aacgaacttt
    13801 acaggtacga caggattagc tgttattact ttggaaaaag cattttttat tactgatttt
    13861 cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa aaatgtcgga
    13921 cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc attagctttt
    13981 gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat cgatgcggaa
    14041 ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga agaagaaatc
    14101 gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat cctgaaaatg
    14161 atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta tatgcgttct
    14221 cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg ttcggctatg
    14281 ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac attggacttt
    14341 ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat tggtgatcct
    14401 ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc agttattgat
    14461 gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga ttatatttca
    14521 aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg tttagagatc
    14581 cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc
    14641 actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga ggatgacttg
    14701 ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt gatcatctta
    14761 tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa
    14821 aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag ataataaagt
    14881 tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt tcttaccacg
    14941 tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc aaagggagga
    15001 ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat ttaggagaga
    15061 tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct aaagtagaag
    15121 gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta ggacgtgcag
    15181 ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta gatctttact
    15241 gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag gaacgggtca
    15301 gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc catgtagtag
    15361 ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa gacgaagagg
    15421 aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt gcaggcttta
    15481 tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa cgcaatcgaa
    15541 ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt ttatcttgat
    15601 acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat
    15661 ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat cttgcgtatc
    15721 gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca
    15781 atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa cggtgtttta
    15841 tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt ctatgaatca
    15901 tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca aaaaatatct
    15961 gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa aatgcaggca
    16021 gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg attagtcgtt
    16081 ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga acgtgtagca
    16141 aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc tgaagacgaa
    16201 ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat attggtccaa
    16261 ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg ccctgaaaaa
    16321 gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa accggacaag
    16381 attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat cgatccggcg
    16441 ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta
    16501 ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa tctggctgaa
    16561 ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt tgtcacaaaa
    16621 gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga tgctaacggt
    16681 aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg gatcactcct
    16741 gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac cgtaaaatct
    16801 gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt cactacatta
    16861 acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt atatttaaca
    16921 ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag cttaaaagat
    16981 gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt acgattccag
    17041 ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga agcgggaggt
    17101 tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt atatcaagaa
    17161 ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg
    17221 ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc ggttatccgc
    17281 gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt gttcccaaca
    17341 gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag agtagaagag
    17401 atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga ggatttatgg
    17461 ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc ggtgatttca
    17521 tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt tcgggctgcc
    17581 actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt gatgcgcatt
    17641 caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg caaacaagaa
    17701 cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt
    17761 cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca gcaaatttat
    17821 catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt ggctccgctt
    17881 tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt agaagagaaa
    17941 atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca atctcttgat
    18001 ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt aaatgatcaa
    18061 gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca ttatcaagat
    18121 ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata tggaaaatcc
    18181 aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag aacaaggaga
    18241 cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa gcaaacattg
    18301 ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag aatcaaatga
    18361 agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac ttttcagcta
    18421 ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat acaacggata
    18481 gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga atacgtccct
    18541 tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc gtctatcaag
    18601 tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat ttgcctgcga
    18661 tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc ggcgaagcat
    18721 tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc agtatggcac
    18781 acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt gcgggatcac
    18841 aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag ctttctttag
    18901 aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat gccttgctgg
    18961 ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa cgattagccg
    19021 aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt ggtacaacga
    19081 aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt
    19141 tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc gccaacaaga
    19201 tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg cgccagatga
    19261 tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg tagatgtatt
    19321 agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag tgatggctgg
    19381 tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag
    19441 tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg gattaaaatt
    19501 agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt tagatattgg
    19561 tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc
    19621 tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta gagtcgtcgt
    19681 aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag gaattccaga
    19741 agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc cattacatgc
    19801 tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg aagcaggcag
    19861 agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa aagttttaga
    19921 agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa gttattctcc
    19981 gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag tacccgaaag
    20041 cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac acgaacagtt
    20101 tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat tcttctttat
    20161 tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc gaagacgtaa
    20221 tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa
    20281 agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact caagctacga
    20341 tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat ggcggctacc
    20401 gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa aaactattaa
    20461 aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa acattgccgg
    20521 gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa ttattttcga
    20581 ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag gcagttttat
    20641 tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc tacaagaaat
    20701 cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg aaggaatgac
    20761 cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt taggtctgtt
    20821 agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat gtgtgcttga
    20881 agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg aattagggat
    20941 cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg gaaaaaatgt
    21001 ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg gcagttattt
    21061 agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat cacatcttgc
    21121 tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt atcagcaaga
    21181 atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc
    21241 ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg ctgctgattt
    21301 gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca attatcaaaa
    21361 aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac aaaacagtgt
    21421 agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag atgtggagta
    21481 tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg caatcggcga
    21541 tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag aagttacgca
    21601 acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg agtcaatatt
    21661 ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag aaggtcaatt
    21721 ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac aagccgcaga
    21781 tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc tgcatgaatt
    21841 gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag aagaaaatag
    21901 acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca atccaggaga
    21961 gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc
    22021 tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg atgaagtgga
    22081 tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc aaatatcgaa
    22141 atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg ccgactatca
    22201 atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt cagagttgaa
    22261 aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga ttacaaaact
    22321 cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag ccacctcgca
    22381 gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg acactcatta
    22441 cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg acaaattttg
    22501 ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac ctcgtcatta
    22561 attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt ttttataaaa
    22621 acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt aatagaacta
    22681 gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt ccatagatac
    22741 tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt ttcataatat
    22801 tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct actagtttta
    22861 attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca cgtaaatgat
    22921 ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc aatgcatcta
    22981 aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc atgatttgat
    23041 aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg cattgagatt
    23101 gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga
    23161 tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact ttctttaaaa
    23221 acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa ataatagaga
    23281 gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa ggatattgta
    23341 acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta cgggaatttt
    23401 ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct
    23461 gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg tcagtttcct
    23521 ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg attttcctcc
    23581 tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa tgtgtttgta
    23641 agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta aagtatatac
    23701 agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt cacaccactt
    23761 atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa
    23821 ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat tccttttttg
    23881 tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt accgtttttc
    23941 tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata ttattatgaa
    24001 gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct gttgacaatc
    24061 gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa aaaatagaaa
    24121 agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa atgtttatga
    24181 ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg gattgtggta
    24241 gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg aattccaaca
    24301 taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag aacttggcga
    24361 aaagtttagt ggaggaatgg ggggctttca atgttgatgg gtgaattcca acataatatt
    24421 gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt
    24481 gttctgacac gcggattgga tggctgtctt ttcggctatc cgatgagcga atgggaaaat
    24541 ctggaagcta aactgaacga aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga
    24601 ttcttctatt cggctgccac tgaatgcgaa cttgataaac aaggacgaat caatataccc
    24661 agcacgttaa gaaattatgc agctttgaca aaaggttgcg tagtcatcgg tgtatctaac
    24721 cgaattgaaa tttgggatga gacacgatgg caagattttt ctgcagcagc agaagagaac
    24781 tttgacgaaa ttgctgaaag tatgattgat ttcggactat aagaatagga agaggctgac
    24841 taatgactga aacatttcaa cactatacag tcatgttgaa agaaacggta gatggattga
    24901 acatcaaacc ggatggcgtt tatgtagact gtacactcgg cggtgccgga catagtgaat
    24961 atttattaag ccaactaagt tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag
    25021 caatcgatca cgccaagctg cgtttagctt cttatgtcga acaaggtatg gtcacattta
    25081 tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga gcatgtagat aaggtagacg
    25141 gcattttgta tgatctaggg gtttcctctc ctcagctaga tgaagcagaa cgtggattta
    25201 gttatcatca agatgcaccg ttggatatgc gcatggatca gtcagctcct ttatccgctt
    25261 atcacgtaat taatgaatat tcctaccatg aattagtaaa gattttcttc cgctatggcg
    25321 aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa
    25381 tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt catcccagct cccgcaagaa
    25441 gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt gcggattgca gtaaatgacg
    25501 aactcggtgc cgaagaggcc tctttagaac aggcaatccg gttattgaaa gtcaatggaa
    25561 gaatcagcgt aatcacattc cattcattgg aagacagaat tgtcaaaagt atgtttaaag
    25621 aatatagtac ggtacaagat ctaccaccgg gacttcctgt catacctgac gaattccaac
    25681 cagaactgaa agtcattact cgtaaaccaa tccttccttc agaggaagag ctaacagaaa
    25741 acaatcgatc acgcagtgca aagttacgta ttgcagaaaa aataagaatg aaagaggagt
    25801 agaatgatgg cagaattaaa gaaaatgcaa gattacgaat atgatttacc tgaattggtg
    25861 gatcagccgg aacaacaaat gcctgattct tcttcgaatc aagaaatcat tcttccacaa
    25921 tcaccggcaa aacgattaaa gcagatttct gctttggaaa aaattgctgt ggcaagcgtg
    25981 gcgattgcta ttgttgcttt atgtgtttta acagttatgc tgcgcacaaa tatcagcgga
    26041 gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg
    26101 agtttagaac aagaaaaaaa cgaactttct cgtactgaac ggattaaaca aatcgcagaa
    26161 gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag tgaagtaaat gagtcttaaa
    26221 aataaaattc ggcggtttat ggaaaagaaa aatctaaatc cgatgaacaa tcgcaaaaaa
    26281 gtaggtatca ttttatttgc tacgagtatt ggattgttct ttttatttgc cgtccgtttc
    26341 tcgtatatcg ttattggagg tcatgttgct ggtacctcat tagctgaaaa aacaaaacag
    26401 ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa cgatttatga tcgtaacggt
    26461 gtggcgctcg cagaagatgc ctcctcttat tcaattaaag cgatactctc aaaaacatat
    26521 acctctgggg ataaaaaact gtatgttgaa gaaaagaatt ttgataaaat agcagaaatc
    26581 ctgcataaga atctttctat agacaaaaaa gatgcactga atattttaga ggacggcgca
    26641 aaaaaagagc tgtatcaagt tgaatttggt agctatggga aaaatatctc ccaagaaacc
    26701 aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg ctggtcttta ttttgtcgac
    26761 catcaggctc ggatgtatcc aaatggcgtc ttttcttctc attttattgg atacgcagtt
    26821 ccagacaaag atgagaatgg cttagttggt aaactaggtt tggagtctgc ttacaatgat
    26881 atcttgagcg gtaaagatgg taagattatt tatcaaaaag ataattttca aaatcctctg
    26941 ccaggtacag tggcagaaga agaaaaagct gtcgacggac aagatatcta cacgacttta
    27001 gacagcagac tacaaagtta tttagaaaca ctgatggatc aagtaaatga agaatatcag
    27061 ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg gagaaatctt ggcaatggga
    27121 caacgaccta ctttcaatcc tgaaacaatg gaaggattga ctggaaaaga tgctatctgg
    27181 cgtaatttct tggtacaaga tagttatgaa ccaggatcaa ccatgaaagt tttcactact
    27241 gcagctgcta tcgaagaagg agaattcaac gagaatgaaa ccttccaatc tggaaaaatc
    27301 caagtagaag atgctacgat caatgaccat gactttggtg aaaaaggtgt gctgaccatg
    27361 cgtcaagctc tttcatggtc aagtaacgtc gggatggtca tactggaaca gcgattaggc
    27421 ggacgatggt ataactattt acaaaaactg ggatttggac aaagtactca ttctggatta
    27481 gatgatgaag taaacggtgc tttacctaca ttaaatattg tcgaccgagc aatgagtgct
    27541 tatggacaag cggtaggtgt gacgaatttc caaatgatga aagctttcac ttctattgcg
    27601 aataacggta caatgattca acctcgatat atcagcaaag tggtggatcc ccaaactgga
    27661 gaagagcgaa caactcaaac agaagttcta gggcaacctt tttcaaaaga aacaacagaa
    27721 aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa actacggtag tgcttatggt
    27781 gtatatagtg tacctggata caatgtatcg gcaaaaaacg gaacagccca aatcgcttct
    27841 gatacagggg gatatcaaac aggagataca gcctacctgt attcgattgt ggagatggtt
    27901 ccttctgaag atccagacta tgtcttgtat ctaacaatga aacaccctaa gacatatgat
    27961 cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac gggcaatgga ttttaaagaa
    28021 actgaagaag attcagatac agaaacaaaa acagaaaaag tatccgtagc cgattatcgt
    28081 aatcttgaag cggacgttgc agcagctgat gctcaaaaaa gtggactgca acctgtagtg
    28141 atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca acggtgatca gttgatctct
    28201 ggagaaaaac tgatcctata tactggtggt gataagctga tgcctgacgt aacaggatgg
    28261 tcaaaagcag atatcatgaa gctggggaaa atcctcggta ttgaagttag ttttgatgga
    28321 gatggttatt gtgtcaaaca agaacttgct ccatatgaaa aaatcactga agacaaactt
    28381 agttttactt tagaagaata agaagagaaa taggagagaa aagagcatgg aatggacaca
    28441 agcattgatt cccattgtca gcagttgtgc aatgacaatt gccgcaatgc cgctatttat
    28501 tggttatttt caaatgaaaa agcaaggtca ggcgattcgt gaggagggac caaagtggca
    28561 caatagcaaa gccggaactc ctacaatggg cggcttagtg tttctgatcg gctctatttt
    28621 gacaggaatt tgggttggag cttggcaaaa acagctaact cctactcttt tcatcttgct
    28681 ttttgtatta gcgttatacg gagtaatcgg ctttcttgat gattttataa aaatattcaa
    28741 gaaaagaaac atgggactga actcgaagca gaaattgatt ggacagattc tagggggaat
    28801 catcttttat cttgtttacc gttctgaagg ttatccaggt gtattgaatt tcttcggact
    28861 agatctacct ttaggaatca tttatggtat tttcgctctc ttctggcttg ttggattttc
    28921 aaatgcagta aatctgacag atggcatcga tggattagtt gctggcttag gaagtatctc
    28981 ttttgcagct tacggtctaa ttgcttggca tcagcatcag tatgatgtat taatcatttg
    29041 cttaagtgtt ttaggaggtt tactgggttt cttcgtctac aacagaaaac ctgccaagat
    29101 ttttatggga gatgtcggtt cattagcgtt gggcggactt ttagcggcaa tttctataat
    29161 gctaaaccaa gaatggacat tgcttttgat tggtttgatc tatgttatgg aaacagcaag
    29221 tgtgatgctt caagttactt cctttaagct tacaggaaaa cgaattttta aaatgtctcc
    29281 aattcatcat cactttgaaa tgtgcggatg gtcggagtgg aagattgata cagttttttg
    29341 gttgatcagc attgtgactt cgttgatcac attatggatc gtttggtagg agatgacggg
    29401 aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct tgtcttagga ctcgcaaaaa
    29461 gcggagtaag tgcggccaaa ttattacatg aattgggcgc acttgtcacc gttaatgatg
    29521 gaaaaccttt tgacgaaaat ccggaagcac aagagctgct ttctttaggg ataaaagtaa
    29581 taacaggtag tcatccaatc gaattgttgg atgaagaatt ttcattgatg gtcaaaaatc
    29641 cagggattcc ctacagtcat cctttagtag caaaagccca agaaatgggt attccggtaa
    29701 ttacggaagt tgagctagct tacgaagtag cggaatgccc aattattggg attacaggaa
    29761 cgaacggaaa aacaacgaca acaacgatga caggactact actgaatgca ggagcagatc
    29821 aaggaattgc acgtttagcg ggtaatattg ggtatccagc aagtggtgta gcgcaagaag
    29881 caaaatcaga agataaaatt gtgatggaat tgtctagctt ccaactcatg ggaatcacag
    29941 atttccgccc tcatatagcg gtgattacca atatctatga agcacatatt gattatcatg
    30001 gtacaagaaa agaatatgtc aaagctaaat ggaaccttca aaaaaacatg acagaaaaag
    30061 attatctgat ccttaactgg aatcagtcag aactacaaga attagcgcaa agaacaaaag
    30121 cacgcgtact gcctttctcg acaaaagaag tgttggaaga cggtgtgtat gctgacgatt
    30181 actcaatata ctataaaaaa gaaaaaataa tggaaatatc tgaacttggc gtaccaggta
    30241 agcataatgt agaaaatgca ctagctgcta tttctgtagc aaaattgtat ggtatatcaa
    30301 atgaagctat acgagaaacg ttgcattttt tccatggcgt accacatcgt acgcaatatg
    30361 taggagaaat ccaaggcaga aaattttata atgattcaaa agcaaccaat attttagcga
    30421 ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt gttattggct ggcggattag
    30481 acagaggaaa tacatttgat gaattaattc cttcattaaa aggaatcaaa gcaatggtcg
    30541 tatttggcca aacaaaagaa aagttgatgg atgctggaaa aaaagcaggc atcgagacaa
    30601 tagtaacagc cgattctgta gaacaagcag ttcctttagc attagaaaac agtacagatg
    30661 gtgatgtagt tttattatca ccagcgaatg ccagctggga tcaataccca aattttgaaa
    30721 ctagaggaaa cagattcatg gaagctgtga accgattaaa atagaaaaag tagaagcgag
    30781 cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag ggggacatat ttatccagct
    30841 cttgcctttg tcaattatgt aaagactcaa gaaccgaatg ctgaattcat gtatgtggga
    30901 gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag gaatgccttt ccataccttg
    30961 gagattcaag gatttaaacg aaaaatttcc atgcataaca tcaagacggt ccgattgttt
    31021 cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt ttcaaccaga tatcgttatc
    31081 ggtactggag gatatgtttc cggagcggta gtgtacgctg cagccaaaat gggtattcca
    31141 acagttatcc atgaacaaaa cagtgtacca ggtgttacca ataaattttt aagccgatat
    31201 gtggatcgga tcgctctttc atttaaagat gcggcttcct tttttcctga aaacaaagca
    31261 gttcttgtag gtaatcctcg agcacaagaa gtagcagata ctaaaaaatc agatattttg
    31321 aaaacatttg atttggatcc agaaaagaaa acagtcttga tttttggcgg tagtcaagga
    31381 gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg aatatattcc ttcttcttac
    31441 caagttttgt atgcttcagg agatagatat tatgaagaga tcctaaataa aataaatcct
    31501 gaaatacaaa atattagtgt ccagccttat atcaaaaata tggcagaggt catggcttcc
    31561 agtgatctgc tggtggggcg tgctggtgca acttcgatcg cagaattcac agctctgggg
    31621 ttaccggcag tgttggttcc cagcccatat gttacgaatg atcatcagac taaaaatgca
    31681 atgagtcttg ttcacgcagg agcagcaaaa atgattgcag ataatgaact aacaggtgaa
    31741 tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag aattgcaaaa gcaaatgtgc
    31801 cgagcttcta aagaacaagg aatccctgat gcttcaaaac gtctttatga cttagtaaaa
    31861 caaataatca aaaaataaag gcggtggtct tagtgagtaa gaaaaaagaa ccaaaaaaat
    31921 tgtctcatcc gtcttctgat gaaaacaatt tgactccttg gcaaaaggct aaccgaaaat
    31981 atctggcaga acaagagatg aaaaaccaag agccaaaaaa agaagacata acattagata
    32041 cacccctttc agattctgaa gaagaaaaaa cggttcaaga ggaacagcaa gaagaaagcc
    32101 agtccgagaa aaaaatcgaa ttttttgaag agatcaagca aaagaaaaac ggtggaccat
    32161 ataacggttc ttttttgaac cggctgccca atttaaaaag tcaacggcac aaagtactat
    32221 ttcgtcggtt agctttgatc atcactgtat tgggtatacc gctgattttt ttgatctatt
    32281 atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc aggcaataag acagtcaaca
    32341 gccaagaaat catctctgat acaaaattgt cattaggaga aaatgtttgg ggacagtatt
    32401 ttcatcgatc tacttatatc gatcgcctta aaaaagcaca gccaagaatc gaaacagcaa
    32461 atatacattt caaagggatg aatgaattcg atttggatgt aacagaatat aaagagattg
    32521 ctttgatcgc aaaaaacaat caatactacc cagtgattga aaatggaaca gtattagatg
    32581 aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga agagttcaaa gactctgcaa
    32641 aaatcaaaga attaacaaag caatacaatc agttatcatc agaattacaa aaagcaattt
    32701 ctgaaatcaa gtacacgcca agagcaagca ataaaaatct gatccaattg aatatgaatg
    32761 acggcaatca agttattgta aatatcaata atcttgcgaa ccaaatgaaa tattattcgc
    32821 aagtagccaa agatatggat gaaaaaggtg tgatcgacat ggaagttggt atcttctcgc
    32881 atccttacgg tagcactcaa gaaacggaag aatcgggcca atcggaagaa acaactgaaa
    32941 gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga ggcagaaacg caaaaagcac
    33001 aaacacctgg atcgtctagc acaaacggat catctgattc aaatgattca agtgaaacaa
    33061 gtcaaataga agataatcca agtacaactc cgtcaagttt aaatgaaaat tgataaaaca
    33121 actttaaaaa gtaagaatgt actttctaaa aaccagtaat ttatggtaga attggaagca
    33181 gtgaatatat ctattaataa gtatatttta aaacggacgc tagttgttcg cttgtatatg
    33241 gaatagtaag taggagggaa tcccattcat ggcaaaaaca ggaatgtatg taggccttga
    33301 tattggaacg acatctgtta aagttgtcgt ggctgaatat atcgacagcc aaatgaatat
    33361 tattggagta ggaaacgcaa aatcagaagg gattaaccga ggcattatcg ttgatatcga
    33421 taaaacggtc caagcgatac aacgagcagt acgacaagca gaagaaaaag caggtatcca
    33481 gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt gaagtagaaa attgccaggg
    33541 aatgattgca gttaacggag attcaaagga aatcaccgat gaagatgtca gaaatgtggc
    33601 ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa atcgtgtcaa tattgccaca
    33661 agattttact gtcgatgggt tcgagggaat caaagatcct cgtggaatga tcggcgtaag
    33721 attagaaatg tacggtctgt tgtttacagg acctaaaacg atcatccata acatacgtaa
    33781 atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg attactcctt tagcgctgac
    33841 tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg atcgtcattg atatgggtgg
    33901 cggccaaaca acaacggctg ttatgcatga taagcaattg aagtttacca atcttgacca
    33961 agaaggtggc gaatttgtca ctaaagatat ctctattgta ttaaatacgt catttaacaa
    34021 tgcagaagcg ttgaaaataa actacgggga tgcataccca gaacggacat caccagatga
    34081 agaatttcca gtagatgtga ttggtcagtc agaaccagtg aaagtcgatg aacgctactt
    34141 gtcagaaatt atttcagctc gaatggaaca gattttcaac aaagcaaaag atgcattgga
    34201 ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg acaggcggag ctgctagtct
    34261 tcctggtgtt gtggacttgg cacaagaaat cttcggcgta aatgtaaaat tgtatgttcc
    34321 aaatcacatg gggctgagaa acccggtatt tacaaatgta ataagtatcg tggattattc
    34381 agcaaacttg agtgaagtgt atcaattggc aaaaattgct gttacgggtg aaacctcagc
    34441 agctcgtcaa atggtagttg aacaagaaac aacaaacaca tatgagagct atgaagcacc
    34501 tgaagaaaca atctacgatg agccggaacc aaaagagtcc ggagaaaatg taaaaaataa
    34561 aatcaagggt ttctttacaa atatttttga ttaattcatc ctgggaggaa taagatatgg
    34621 aattctcaat tgataataat atcaatgacg gcgcagtaat caaagtcatc ggagtcggtg
    34681 gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa cgttaaaggt gttgaattca
    34741 ttacagccaa cacagacgtg caagcattaa aaaattcaaa agctgaaaca gttatccaat
    34801 taggccccaa atatactcgt ggtttaggtg ctggttctca gccagaagtt ggacaaaaag
    34861 cggcagaaga aagtgaacaa tccttacgcg aagctttaga cggtgcagat atgattttca
    34921 tcactgcagg aatgggtgga ggaaccggta caggtgctgc accaatcgtt gcaggtatcg
    34981 cgaaagaatt gggcgcattg actgttggtg tcgtgacacg tccattcact tttgaaggac
    35041 cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt gaaagaaaat gtggatacat
    35101 tattaattat ctcaaataac cgtttattag aagttgtaga taaaaagaca ccgatgcttg
    35161 aagctttccg tgaagcagat aatgttttac gacaaggtgt acaaggtatc tctgatttga
    35221 tcacagcgcc aggttacgtt aaccttgact ttgctgacgt gaaaacagtg atggaaaacc
    35281 aaggtacagc attgatgggg atcggtgtgg ctagtggaga agatcgcgta gtagaagcaa
    35341 cgaaaaaagc aatctcctct ccattgcttg aaacatctat tgatggagct gaacaagtac
    35401 ttcttaacat cacaggcggt ttggatatga cattgttcga agcacaagat gcttcagata
    35461 tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct aggtacatct atcaatgaag
    35521 aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg tatcgatgaa tcaaaaaagg
    35581 aacgcaagtc tagccgacca gctagacaag cacaaatgca atcacctgct caaaagactg
    35641 tattagatat ggatcaagct aaaccaactt cttcagaaga agaaaacagt tttggcgatt
    35701 gggatatcag acgtgaacaa aatgttcgtc ctagagttga tgattccaat tttgacaata
    35761 tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac aaaatctcat ggcgatgatg
    35821 aattaaatac gccaccgttt ttccgtcgta aaaaataaag gagaggaaga gcacatgatt
    35881 gctgacaact tgcagaaaat caagcaagaa attcaggatt cgtgtgctct tgttcatcgt
    35941 gatccgaaag aagttacatt agtagctgta acaaaatctg ttgaatccgc tcaagctgaa
    36001 gaattggtta gagctggcgt tatgaattgt gcagaaaacc gagtagataa attattagaa
    36061 aagaaacaag atttatttga ttttcctgaa ataaagtggc atttaattgg taatttacag
    36121 cgcagaaagg taaaattgat tgtaaatgaa attgattact ttcatgcgtt ggacagttta
    36181 agattagcag aagaaatcca gaagcggtta gagaaagaat tatcctgttt tattgaagta
    36241 aatgtttctg gtgaggaatc aaaacatgga atttcaccag atgagctttt accgtttgtg
    36301 gaatctttag ctgcctgcac aaaaatcaag gttgttgggt tgatgactat ggcgcctaaa
    36361 gaagcttctg atgagcaaat ccataaagtg tttggtacgc tgaaagaatt gcgtgatctt
    36421 gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat taagtatggg gatgagtcaa
    36481 gactttcaaa ttgcgattga agaaggtgca acatttatcc gtgtaggaac agcgctattc
    36541 aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac agaggagcaa ttgctaattt
    36601 tttcggatta gctgatgagg aagagtacga ataccaagat tatcctgaaa caacaaaaaa
    36661 ggctgtacag ccaactaaaa aaaatacaac acagaatcaa tcaactcatt ctgtatctgc
    36721 tgcgaatact catgaaaaac aaaaaacggc gacttcacgt cctgtaacgc atgaaagtat
    36781 tcagccggct gaaaagaaaa aagtaattga actacatcaa tcacctacaa aaactgtagc
    36841 gaaagaaaga acagcttcaa gaacctctac acaacctaaa gcaaatcttg ctggaaaaat
    36901 cactgtaatg gaaccaagag cttattctga agctatggcg attgctaaac gaatcattgc
    36961 tggagaagca accttagtga attttcattt agtagaagaa aaacaagcga gaagaatcgt
    37021 tgattttcta acaggaacag tttatgcact agatggagat atcaaacgtg ttggagatga
    37081 gatttttctt tgtacaccga ctgggatgga gattgacagt tcaaccgctc aatcttttgc
    37141 agatgcgaat ttgtttgatc tgtaagagga ggaactacga tttatactat tgcactatta
    37201 cttcatttac ttcaacgggc agcctatttc tatagcatta tcttgatggt atatgcattg
    37261 ctctcttggt ttccaggcgg gtatcaatca gggctaggac gattattagc taagatctgc
    37321 gaaccgtata tcagtttgtt cgatcgtctg cctttgagga ttggaccctt ggatttcaca
    37381 attttagggg caatcctgat cttgaactta gccgtacaag gtttagcaca gatcgttata
    37441 tggataactg tccatttagt ttattgattg aagcaacaaa atctttattc tcagaataga
    37501 taggagggga aaattttgga tgcaaatgta taccagcact ttcgggcaga tgaacgtaca
    37561 tttatcgatt cggtaggcga ttggattgaa caggttcaag gacaatatgc accctatctg
    37621 accgaatttc ttgacccaag acaatcctat attttagaaa cactgattcg acaaagcagt
    37681 gatttgcgtt tcatgtttta tggcggatat gaacaggcag aaagaaaaag atgtttgatt
    37741 ttccccgatt attacgaacc tgtagaggaa gattttgaaa cagcattgta tgaaatccat
    37801 tatcctagca aatttgccag cctaagtcat gggaaaatcc ttggaacttt agtgggaacg
    37861 gggatcaaac gctcttactt tggcgatatc atctcggacg gtgatcgctg gcaagtattt
    37921 attgcaaatg aggtggatca ttttgttgct gcacaagtaa caaagattgg aaaggttgct
    37981 gtacgcttgg aaccaatcaa atacacagaa atgattatgc cgaaagatag ctggacacaa
    38041 gaaagaggaa cagttagttc catgcgacta gacagtgtga tctctgagat ttataatatt
    38101 tccagacagc ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa ctggacagaa
    38161 aatacaagac ctgattttgt acttgaacta ttagatatta tttcagtcag aggttatggc
    38221 agagtccaga tccaagaact tgaggggaag acgaaaaaag aaaagtacag ggtactgttc
    38281 ggtgtattac gtaaataaaa taaaaaggtg gtttactcat ggctttaact ccattagata
    38341 tccaaaataa gacattcccg acaaaaatgc ggggatataa tcaagacgaa gtagatgatt
    38401 tcttagattt agttgttcgt gactatgagg aattgactca aagaaatcga gaattagaaa
    38461 aagcagtcaa acattctgaa gaaaaacttg agtacttcaa tgaattaaaa gatgctttga
    38521 accaatcaat catcgtagct caagatacgg ctgataaagt aaaaacaagt gcaagcaagg
    38581 aatccgaagt gatcgttact tctgcacaaa acaaagcaga tgagttagtt gcgaacgctg
    38641 aaaaacgtgc acatcaatta acaactgatg cagaagaaaa agcaagaaaa atcttgacag
    38701 atgcaacaga aaaagcaaga cagcttgcga cagaaactga agatcttaag aaaaaaacac
    38761 gagtattcca tcaacgtatc agcttgatgc ttgagtctca attagaacaa gtgaaaagtc
    38821 cggaatggga tgaaatttta caaccattct ccagctacgt gactgacagc catgaagtga
    38881 tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga tactgatgta aactcagaca
    38941 caggagtcat agaagcacct atgacggcat ttgatacaca ggctattgat atctcgatcg
    39001 tgcctggtga aaatgaaaac gagtagaaca gaaaagcact ttgttattct taaagcgagc
    39061 cgatgagagt ggaagttcgg taagacactg catggtgaaa gatcctctac ttgagacctg
    39121 cttttccgaa attagagtaa gaagcagcgg ctgacctcgt tattggtcac agagagtttg
    39181 cactagatag atgcaactaa atttgggtgg taccacggca tgttcgtccc ttgcggctga
    39241 acatgctttt ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg aaaatgaaag
    39301 aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg taatttgcca aaccgagaag
    39361 gcgagtggca aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa gagttgaacg
    39421 aaggaaaacc tagctttgtt cttcatgatg gacctccata tgccaatggt aatatccatt
    39481 taggacattc attgaataaa atcagtaaag acattatcat tcgttcaaaa tcaatgtctg
    39541 gctatcgtgc accatatgtt ccaggatggg atacacatgg attgccgatt gaacaagtat
    39601 tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc agaatacttg gaaaaatgtc
    39661 gtgaatatgc actttcccaa gtagataagc aacgagcaga tttcaaacgc ctaggagttg
    39721 caggagattg ggaacatcct tatgttacat tagatccttc ttatgaagct gcacaagtac
    39781 gcgtatttgg taaaatggct gaaaaaggct atatttacaa aggattgaaa ccaatttact
    39841 ggtctccgtc aagtgagtct tctttagcgg aagctgaaat tgaatataaa gatgtaaaat
    39901 ctccatctat ttttgtagct ttcaatgtca aagacggcaa agatattctt gatgaagata
    39961 cagcttttgt catttggacg acaacaccat ggacattgcc agcaaaccaa ggtattgctg
    40021 taaatccaaa atttacgtat gtattggtgg aagccgatgg ccgtaagttt gtcgtagcga
    40081 aagatttgct agaaacagtc caatcagaaa tcggttggga aaatgtaaca gttctaaaag
    40141 aatttgctgg acaagaaatg gaatatatga ctgcaagtca tccattctat gaacgtgaat
    40201 cacttgtgat tttaggcgat catgttactt tagacgctgg tactggatta gtccatactg
    40261 ctccaggaca tggggaagat gactatatcg ctggtagcaa atataaacta tctgtagtgt
    40321 cacctgtaga ctcaaaagga ttgtttacag aagaagcacc tggatttgaa ggtatctttt
    40381 atgacaaagc aaatcctatg atcactgatc tattaaaaga aaaaggagct ttgctgaagc
    40441 tagatttctt tactcatagt tatcctcacg attggcggac gaaaaagcct gtgatctatc
    40501 gggcaactcc acaatggttt gcatctatcg ataaattccg tcagaatatt ttggatgaag
    40561 tagaaaaagt agagtgggtt atcccatggg gtaaaacacg tttgtataat atgatccgtg
    40621 accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt ccgattttct
    40681 atgctgaaaa tggagaagca atcatcacac cagaaacaat cgaacatgtg gcaaaattat
    40741 ttgctgaaca tggatcaatt atctggtttg aaaaagaagc gaaagaactt cttcctgaag
    40801 gatttaccca cccaggttct cctaatggcg aatttacaaa agaaaaagat atcatggatg
    40861 tctggtttga ttctggttct tctcatgaag ctgttttacg tcagcgacca gaattatcat
    40921 tcccagcaga tatgtattta gaaggatcag accaatatcg tggctggttc aactcaagta
    40981 tcacgactag tgtggctatc aatggtgtag caccatacaa atcagttatt tctcaaggat
    41041 ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc attgcaccag
    41101 aaaaagtgat caaacaattt ggtgcagata tccttcgctt atgggtatct agtgtagatt
    41161 atgaagcaga tgttcgtgtt tctatggata tcttaagtca agtggcagaa gtatatcgta
    41221 aaatcagaaa tacgatgcgt ttcttattag cgaatacaga agatttcgat cctaaaaaag
    41281 atactgtttc gtacaatgat ttacgttctg tagacaaata tatgaccgtt cgattgaacc
    41341 aagtaatcaa agaaatccga gaagaagggt acgacaaata taacttcatg catatttacc
    41401 gtacagttat gaacttcttg acggttgatc tatcgtcatt ctacctggat tttgctaaag
    41461 atgtagttta tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa actgttttct
    41521 atcaaacagc tgtagctttg acaaaattat tgactcctat cattccgcat acagcagaag
    41581 aaatctggtc attcctgaaa gaagaagagg agtacgttca gttaagcgag tttcctggtt
    41641 atgaagagtt tgcaaaccaa gaagaattga tggatacctg gactgctttc atggacttcc
    41701 gtgataatgt cttaaaagca ttggaagaag caagaaacag caaattgatt ggtaaatctt
    41761 tggaagcaaa actaactgtt tatccaaaac aacaagttcg tgaaatgtta aaagctttgg
    41821 atgcagatat tgcacaatta ttgatcgttt caccagacta ctttgaagtt atggcagcag
    41881 atgaacaagt gccagataac gctatggtat ttgatgatgt tgctattaca gtggaaaaag
    41941 cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga gtagatgaaa
    42001 aacttcctca tctatgcggt cgttgtgcaa aaatcgttga agacttctat cctgaagctg
    42061 tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt tctttgtcac
    42121 accccctttt gttatcaatc gtttaatttt ccacgttcac gataccagtt atctggttgc
    42181 cagatccatt cgaatccgtc tttttcaagt agttcgaaag ctgctttagg tcccattgat
    42241 ccagctttgt agttgggaaa atccggttgt gtttggtccc aagcttttcg aatgacatcc
    42301 acgatatgcc aagattgagc gacttcttcc caatgagaga agttcgttcc atcaccattc
    42361 aacgcatcta atagtaattt ttcgtaagct tctgggctat tttcaaccat ttctgcacta
    42421 tttctaaatt ccaatttgat tggttccgtt tcaaatcctt gccctacttc tttcccattc
    42481 aaggataaag aaaatccttc agttggctga atatagatcg ttagaacatt aggtggaagt
    42541 gtcgtatcgt cacatggttc atcgacagat gttttgaata cattaacagg aacttgtttg
    42601 aatacgatgt tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg tacatagaaa
    42661 ggaacacctg accaacggaa gttatcgata gtgaatttgc ctgcgacgaa tgtttcagtc
    42721 tctgattgag catccacttt gtcttcttcg cgatagccct tgaattgtac accatccaat
    42781 tcaccttgag catattgccc gcgaacgaag ttttctaaca cttcttcttc gttataaatg
    42841 cggatagctt tcaatgcttt gatcttttct gtacggattt ctttttcgga aaatgctact
    42901 ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac catgtcttta
    42961 agtgcgccac tatgatcgta gtagcccccg cgatcttcta caccgaggtt ttcgccaaaa
    43021 gtaatttgga tattgtcgat atagcgattg ttccattgag cctcaaaaat attattggca
    43081 aaacggatag ctgagatgtt ttggatcatt tcttttccta aataatgatc aatcctgaaa
    43141 atatcttgtt ctgggaatac ttctctgatt tgctggttca attcaaaagc agactggtaa
    43201 tcagaaccga acggtttttc tatgatcaaa cgattgaagc cattttttgt cataatattt
    43261 tgtgatttca aatgagaaac gatcgttcca aaaaattgag gtgccatagc caaataatag
    43321 acacggttac cgtttaattg atagtgtcca tctaatcggt cagacaattc ttttaatgtc
    43381 tgataatgcg ttgaatcatt tacatcatgg gattgatagt aaaaatggct tgcaaaggta
    43441 gttgcttctt catctgaagg attcaatgca cggattgttt ctttgacaac ttcacggtag
    43501 tgctcatcgc tccagggacg gcgagcggtt ccgattacag caaaatgctc gcctaaattt
    43561 ccttttctgt aaaggcggaa caaagacggg tacaatttac gctgggctag gtcacctgta
    43621 ccgccgaata tcgtaaacaa tacatttttt tcattagaca tgtggataca ctcctttata
    43681 ttttatcgac aaaaatcgtg ctggccgcct taaagctgac ggggattttc tttctatcat
    43741 tttggatagt gatcggtcct tcataagctg cgatgtcaac gatctcatat tcttcgttga
    43801 tattcaggtc aatagtgact aagtaatcca acaattcttt ttcatcaagc actcgtgcga
    43861 tacgcacttt tgtaccgaca ggataggact ccaatgtttg tcggcgttct tcatggacaa
    43921 cttcctcttt tttaggaatc gatcctccat gaggacaatg ttcaggatga ttcaaatagt
    43981 tttctaaatg atctgcaaga tgttccgaag tcacatgttc caatacttca gcatcatcat
    44041 ggacttcatt ccagctatag tctaaatgtt caactaaaaa aacttcccat aagcgatgtt
    44101 tgcgaatcag actgctagct ttttgcagtc cgcttgcagt caattgtaca ccttgataag
    44161 gggaatgttc tacaagctct tctttcacaa gtttagaaat catttcactg acagatgcag
    44221 ctgagacatc tagactagat acgatttgtt tattgttgac tttcgttgta tctccaccta
    44281 gttctaaaat gattttcaaa taatcttctc gatttggtgt catattcgat catcctttca
    44341 tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg ttaattgact atttttctaa
    44401 ataaaaaaca aaggattttt agacgttttt acaacaaaaa acgaaagatg atagagaaga
    44461 ctcattcttt cgtttataaa ggatatatat atacattggg agggatctat ttttcagata
    44521 acaacttata tgaagtgtct gcagattcgt cataaagagt ttgttcggga taagaaaaga
    44581 taagataaaa accatactga tcacgtacga ctaaacgata ttcatctatt tctaccacag
    44641 atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc agatgtaatc tgtaattgtt
    44701 gtttttgctg gctatcacta aagaaccatg taccttgata ctttgagagt ccttctgact
    44761 tttccgcttg ttttttcttt ttcaagtaaa taaaacttga gagaacggta atgagtgaac
    44821 caagaaaata gatcattttt tttatcacca aatcactcct ttatgagaaa ataatcgcat
    44881 agacatattc tttcttcttt atttcattgt accgaataaa agctaaaatt tctctaagac
    44941 tttcgactgg tttttttact gtttttctac aaattatgat aggatacata gtaggaagaa
    45001 aggtgacata atttgaagta tcagcaagtg gaaatagctt tttttattga ccgcccgaat
    45061 cggtttatag cacattgtat gaaaaaagac ggagaaataa ttattgcgca tgtcaaaaat
    45121 acaggtagag gaaaagaggt cctgatacca ggcgctgaag tggctattgt atttgctcct
    45181 ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa aaaagaaggg tgactggttt
    45241 aatatcgata gtcaattgcc taatcggctg gcaattgatg gtattttaga cggcacgatc
    45301 cttttaccag gattaactgg tccatttacg gtatacaagc gtgaagtaac ttatcttcac
    45361 tccaaatttg atatttattt agagacggtg gtcggacaaa aagcatttgt tgaagtcaaa
    45421 ggtatgacac ttgaaaataa agcgattggt gcttttcctg atgcgcccac actcagagga
    45481 ttgaaacata taggagaatt aacgtatgcc gcacaagatg gttatgccgc ttatgtcttg
    45541 tttattgctc agttcgaaca tcttcatctg gcaacgatcc atgaagaaat gcaaccagca
    45601 ctagctgata tggttcgaca tgcgcagcaa agcggagttc aaatcctcgc ctataattgt
    45661 caagtatcgg cagatgaggt aactgtcaag caggccattc catttgatgt agatatccct
    45721 tttgaagatc ctatacaata gaaagaaggt aaaataatga tcaacttagg tattatcgga
    45781 acaaattgga ttactcatca atttgtccaa gcagctttaa gcacaaaaaa atatgaattg
    45841 acagcggtat attctagaca tttggaaaaa gcacaggaat ttggttctca ttatgaaggg
    45901 gatatcgaat tcgcaacaga tttgaagact tttttcaata ttactcatat ggacaccatt
    45961 tatattgctt ctccgaatag tcttcacttt agtcaagcta agcaagcaat tttggcagga
    46021 aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa aagaaatgga tgaaatcatc
    46081 catttggcaa atgaacacaa tgtgtatttt tttgaagcag caagaaatat ccatgaatca
    46141 ggttttaaaa agattgccga ttacttacca ttaaaagacg agataatcgg tgcaaatttc
    46201 agctatatga aatattcttc gcgctatgac caagtattag atggagaaga accgaatatc
    46261 ttctcgactc atttctcagg tggtgcattg atggatttag gtgtttacct tgtatatgca
    46321 gctgttgctt ggtttggaat gccaaaagaa gtccattatt tcccaagaaa gatttctact
    46381 ggtgtagatg gtcttggatg ggggatctta cgttatgaga attttgatgt atcgatccag
    46441 ccaggtaaaa tcggtgattc ttatttaccg tcagagattt attttgactc aggaacgcta
    46501 gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc atgatcgcgc gcataaagaa
    46561 atcattgatt tggcggtaac agctgcagaa aatccaatgg cagatgaagc agaggatttt
    46621 gccaatgtca tggctcatcc gaaagatcca gcttggggat tgttgtatga agagtggatc
    46681 gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa gaaaaaatgg cggcatcaaa
    46741 ttcgatgcag ataatgagga agatttttaa tgaacgaatt tgtacaagta tttcccgaaa
    46801 attggcagaa aaaatatgag gataaagggt ttatctcacc ttctcttatt caacgagcag
    46861 tttttcagcc actaagtaat aaacaaagta tagtagcggt ttctcctaca ggttcaggta
    46921 aaacactggc ttatctttgg cctctactac taaacgtgga accaggtgaa gcaagtgcgt
    46981 tggtcatttt tgcttcttct caagaactag ctattcaagt agcagatgtt gcacgtgaat
    47041 ggggaaaaga caaagagttg aaagtccaat ctttggtggg cggagccaac gtcaaacgtc
    47101 agattgaggg tttgaaggaa aaaccagaaa tcttgattgg tacaccagga agaatattgg
    47161 aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa aacaatggta tttgatgaag
    47221 cagaccagct atttgacgct gggaacagcc aaatcattga tcaaattctt catcaagctc
    47281 caacagagta tcagctagct tttttttcag cgacagcaga tcgttccctt gaaagcattg
    47341 aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac agcagaggac gattctcgaa
    47401 aaggactgag acattacttt ttacgtgtac cgatacgaaa gaaagatgac tacttgagac
    47461 gtctcacgca tatagaagat tttcatggtc tgattttctt taatcaactg actgaattag
    47521 gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt aggaagtctg gcctctgatc
    47581 agaacaagct actccgaaaa gcagcgctag aacaattcaa aaagaaaatg gtcagagcac
    47641 ttttgaccac agatgtggcg gctagaggtt tagatattac aggtcttcct tatgtagtga
    47701 atgcagaagt tccgttaagt gaagaagctt atttgcatcg ttcgggaaga actggtcgaa
    47761 tgggtaatga aggaatagta attacactcg tacaagataa taatctaaga gatttaaaac
    47821 ggcttttacg tccatcaaat ataacacttg aagagatttt tttatacgga ggtaagctgc
    47881 aaactgagca gccaaccaaa gaagatgcag ctcctgcagt aaataaaaag tatgcgaaaa
    47941 ctttttctgg aaaggagcag aacccagaat ataacaaaga aaaaataaga aaaaaagtta
    48001 aaaataaatc aaaaaaagag agaaataaag gagcaaggcg caaataagcg cctttttatg
    48061 ctatgattat gaaaacggga acaaaattgg ttgccagagg aggggttatg tgaaagaacg
    48121 tcaagaagag atcttgcagc tattgacaga aaaccaacaa ggattgacag caagtgatgt
    48181 agctgagcgc ttaactatcg atcgaagcaa tgcaagccga tatttaagtg aactatacaa
    48241 ggcccatcat attgtaaaaa ccgctggccg accagtcgtt tactctttgc caacggaaaa
    48301 gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa gtgacttttg aaacactcgt
    48361 tggagaaaat gattctttaa aagtcagcat acagcaggca aaagctgcga ttctttatcc
    48421 accgcggggg ttgcatacga ttatttttgg agaaactggt acagggaagt cgatgtttgc
    48481 tgaatgtatg tatcattttg caatcgattc agagatgctt tctgcagatg cgccatttgt
    48541 ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg ctctttggac acatctttgg
    48601 gatcaaaaaa ggggcttaca caggagcagc tcaagatagt cccggtctaa tagccaaagc
    48661 agacggcggt atattgtttt tagatgagat ccaccgttta ccgccggaag ggcaggaaat
    48721 gctgtttact ttcattgata aaggtattta ccgtccgctt ggagaaagtg cacaagtaca
    48781 tgaagcatct gttcagatca ttggggcgac gactgaatct tcagaaagtt tcttatctac
    48841 gttcaatcga cggattccta tggcaatcac cttgccgaat cttgcttcac gttcactgga
    48901 tgagcgttat gaaatcatct ctctttttat caaacaagaa gcgaatcgtt tgaatcaacg
    48961 tatcgatatc gaaaaagaag cgattcttgc cttcatgctt tatgatgcgg aaggaaatat
    49021 cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa tcttttttac attaccgtac
    49081 tcatcatgag aaaaagctaa tcattcgaaa agaagaactt tcccttcaag tacaaaaagg
    49141 attactgaaa atcaaggaaa taccggaacg tctcgaccga ttcatggaca gcaaaagtca
    49201 gtatttgaca tttgaaccag gatttgctga tgttgtctgg tcacaagatc cagaacgaaa
    49261 catgcaggtc tataacgata ttgaggaaaa agtcacttct ctttccgaaa ccggtgtgga
    49321 gaacattgat ctagagaccc tcatttcaaa agatgtggat gcttatttcc agacatatgt
    49381 caaagaattg actaaaagtt cgattcccaa agatcttttg ccagatgaaa tctggcggtt
    49441 aaccaatcaa ttgtatgatg tggctgaaaa acggttagac cgtatttata atgaaaaagc
    49501 tcgttttgct tttgctttgc atctgcaaag tacattggat cgtgtgagag aaggtcatat
    49561 gatcgtccac ccagatttga ataacgtacg gaaaaatttt aagagggaat tccaggtagc
    49621 aatcgatctt tcaacgatca ttgaagaaga agaacaagtt gagattcctt ttgatgaaat
    49681 cggctttatt agtatgtttc tttctatcaa tctaggagaa agtgagcctg ttaaagaaaa
    49741 caaagtagat gttctcatat tgatgcacgg cagacagact gcttcaagca tgttggagat
    49801 ggcacaagaa ctattaggaa caaaagtagg gaaagctttt aatatgccac tggttatgga
    49861 agttcaaaca atgtatgaag aagtgttaca atatgtcaaa aactatcaag agcatttgac
    49921 gaatggccta ctcttgttaa cagatatggg atcattaaac accttcagtg atttgattta
    49981 tgaagagact ggcattcgga cgaaagctat cacgatgaca agtaccatga ttgtgataga
    50041 agcgatacgg atggcaagtg ttggaagaag cttagaagat atttatcaaa atatccagct
    50101 ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa aagtctgaaa ctttacagcg
    50161 taaaaaagcc gtaatcgtga catgctttac tggtgaagga gtagctgcca aattatatca
    50221 gcgtattatg ccagtgatta atcaaaataa ggtagaactg atccagatgc agtttttaga
    50281 acgtgaatca ttcaaaaaac acatcgatgc cttattggaa gaatatgaga tcaaagcaat
    50341 tgcgggaaca gttgatgttg attatcaaaa tatcccattt ttttcagcct atgacgtttt
    50401 tgatgatgaa aaactaaata ttttaaagcg tatagctgat gacgagatcc cgacagctaa
    50461 aattgtcgat tcacttgctg gatcattaag cgatgtcttt tctattgatg aactgatccg
    50521 ttcattgcaa aagacagttc agcagatcca gaccgatttg tcgattatcg tggaacctgg
    50581 agtagatact ggaatcgtta tccatttagc cttcatgtta gacggattga tcaaacaaga
    50641 aagccatcgg caatttgaga aactgtctat tttctcaaaa atttatcgtt tggaaatgga
    50701 tatcgttcgg gcaaacttga tggcactaga acgaaaatat caggtaaaaa ttccagaaga
    50761 tgatatcgca tttcttacac aaatgtttct tgaaaataaa ataaaaaaat gaacacacta
    50821 atcatactgt ttacacacta aacatctgtg tgcgcactat aaaaaagagc taaaaagcat
    50881 gaaaacgcta tttcagctct tttttttatt ggcatgcttc ttgcgttata taaaagtgta
    50941 aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat catgttagtt tgttcagcag
    51001 ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc agcagaagaa aaagggatgg
    51061 aagccgatat ttttgcggtc tctgcctcag atgcagataa taatttagaa tctaagaatg
    51121 tcgatgtatt attattaggt cctcaagtac gtttcatgaa agctcaattt gaacaaaaac
    51181 ttgcgcctaa aggtattcca ttagatgtca tcaatatgca agactatggc atgatgaacg
    51241 gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata aatcaagtat gcagttgggg
    51301 gtataacaac gtggaagatc aacaaaatct agaagccatc atggggttaa tcatgtttgg
    51361 cggtaatgca aagagcgatg caatggaagc gattgcggct gctaaaatcg gtgattttga
    51421 gttagcggat aggaaaatcg ctgatgcaga agaatcactt atacaagcgc atcatgcaca
    51481 aacagacatg ttgacacaag aagcacaagg aaatcatatg caagtgacat tattgactgt
    51541 tcacagtcaa gatcacttaa tgacatctat tgcgtttact gatttagcaa aagaaatcat
    51601 cgaactttat cgtcgcgtat acagcgaata aatacaactt cttttttgaa cggaagaaga
    51661 aaaatttttg aacggatttt ttattttgtt ataaggagaa ctctctttat ataaaaaact
    51721 tataggagga agtaaaaaat gaacggacta tcaagttgga tggagaaata catcctgccg
    51781 gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt tgcgtgatgc ttttatcggc
    51841 acactgcccg caacaatggc gggatcagtc gctgtaatga tcaatgcaat tattcgtgat
    51901 ttaccgcaac agtttaacag tacctatgct gacagtttag cagcagacaa aggaatcttt
    51961 gccgtattga atgtcatcat cggaatcaat ggttttgtct ggaacggtac actagcaatc
    52021 gccggattga tctttgcctt ttcttggggg tataatcttg cccgagctta tggagtaaat
    52081 gatctagctg gggggatcgt tggtttagct accttgattc aaggtatttc ctttggtcca
    52141 gcattatcag cagcattaga tgtgaatgtt ccttcaaatg tcgcagatgc aatcaatagt
    52201 tctgagattg gtgcaacgat tgcagatggc acactgtcag ttggattatg gggttggcta
    52261 aaactagatc atttgaatgg aaacgcttat tttacagtta tgatcatggg agctatttca
    52321 gtcattattt tttgtaaatt gatgcttgcc aacattacga tcaaaatgcc tgattcagta
    52381 ccaccagcag tatctaaagc atttgcagcc attttaccag cgacgatcgc tttatacgtc
    52441 attgctatta tcaattatat cgtaggtcgt gtagctgatg ggcaactgat cattgatttg
    52501 gtgcaaaaat atattgcaga gccattccta ggattatctc aagggttggg agcagtattg
    52561 atcgtcacag tatttgttca agtattctgg ttctttggta ttcatggacc aaatgtattg
    52621 gcaccagtgc tagagggaat ctggggacaa gcacaactaa tcaatattga tattttccaa
    52681 aaaggttata acggaaaaac tggtacacca gccgtgttag atgcaatcga agatggaaaa
    52741 gcttatatgt gggtccgcgg atcgttcgat gcttttgcat ggtttggcgg atcaggcgga
    52801 acgatcgtct tactgatagc tatcttgatt ttctctaaac gtgcagatta tctaacagta
    52861 gctaaattat ccattgggcc tggtatcttc aatatcaacg aaccagtgat gtttggtctg
    52921 ccaatcgtat tgaatgcaat catgtttatc ccattcttga ttgctccagt agtagcaaca
    52981 gcaattggat ggacagctac ttatcttggc ctagtcgctc cggtatcgca gcaagtcaca
    53041 tgggtagtac cgccgttctt attgtcattc ttagcaacag gagcggattg gcgggcaccg
    53101 atcgtaacat tagtatgtat ggtcgtgact ttcttgattt gggcaccatt tgtcatctct
    53161 gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag atgaacgtga ggagcgggac
    53221 gctttaagtt tcgttcctca taatgaattt tcgaggagga actgaaatga ttacaattgc
    53281 cagtgcaatg caacaagatg aaatcaaaga attgttagaa ggatacaatg aagaaggaat
    53341 gacttttaca tttaaagaaa aacaaggaat caagctgttc tttgaaacaa gtgcagaaga
    53401 tccggaagtt gcggctaaaa aagcaaagga actaatcaaa gcacagcctt ggggaacagt
    53461 cctttatttt caatcaacag ctgtttagaa aatggtttaa aaagatagga tggttgagat
    53521 gataaaaaac ggtttatttt tatgtagtat tggttctttg atcgtgttgt atagtttgaa
    53581 tccacattcg caaaggtatg atttgtatag tatggtcaca ggtctttttc taacaggatt
    53641 aggaatctat ttcttcatga aggggaaaaa aagagaagaa gcaaaggaga aaaataacta
    53701 atggaaatca cgaaaaaatt aaatattcca gcatcgtttt tttacgataa agttatggac
    53761 tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag taactagaaa acagctgaaa
    53821 aatttggagt acgtcaaaca attctcgcaa aataaccggg ctcgcataaa aatcgaagaa
    53881 gtcattgaaa accagtctta taagtttcgt acatcgacaa cgaagaatga attcgtcgtt
    53941 cattatcaaa tcaaaccatt ggacgattcg acctgtgagg ttcattacac agaaaaaatg
    54001 gagtcttatg gcatgcttca aaagttgaat gatatgcttt taggaacaat cttgatgtat
    54061 tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg aagcaacgta ttaaatcaat
    54121 gaaacatggt actatttagt tattgaaaaa tgaagtaagc tagaaggaat ttttcatagg
    54181 aagatagatc tttcctgcga atcctcacta gcttttttgt aggaaggggc agcaaaatga
    54241 caaacaaagt aagagagcta ggtgtatctt cgattgagga aatgtttgat ttagccactt
    54301 atgcatttaa tggggcagat acacctgaaa ggcgagaacg ttttcgtaca cttgcggaga
    54361 attcttggaa ttacggattt tttgatgagg aggatcggct aacaagtcaa gtcatggcga
    54421 ccccttttcc agtgaatttt tacggacaag agtacctaat ggccggcatt ggctatgttg
    54481 cttcttatcc agaagcaagg ggacaaggcg ggatcaatca gatcatggaa aagatccttg
    54541 aagattgccg tgatcgtaaa gtaagcttat cttatctggc gccattctcc tatcccttct
    54601 atcggcgcta tggctatgaa caatcatttg ataaaatcag ttatcaacta aacagtcgtg
    54661 atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg tatggatttt gaagatgcaa
    54721 aagctgcaat cagaagaatt tatgaacaga tgccggaaaa ccaacgagct ggtctgaagc
    54781 gcgaagactg gtggtatacg tataaattca agcaaaaagg caattaccag tttgctttgc
    54841 attttgatga taatgaggaa gcaacgggat atatcgttta tcagcttgca acgtcttcct
    54901 ttattattgc ggagtgggga tttttggatc atgatgcttt ttgcagcctt gttcgcttcg
    54961 taagttctca caatggtgct tttgaaacat ttgaatattc atgcggctac ggcggtaatg
    55021 atcaaaatta tctattagaa aatccatttg cttctgtaat gatcacacct tatatgatgg
    55081 cgaggatcgt agatatttct ttgtttttgg aaaattatcc atttaaaaag agacaagctg
    55141 catttgccct acaaatagaa gaagatcatt atgcgaaatg gaatgaagga atcgtggaag
    55201 taacaatcga aaatggaaaa aatcatctct caaaagtaga acaaacaaaa ctcccaatcg
    55261 ttcgaggaag tatccaacat atcacgcagc tattattagg gtatcgtaaa gtggaagagt
    55321 tggtattcca agaaagaata caggtgacaa aagaatatga agaaacgtta agctcaatct
    55381 tgccacagca acagccaatt cttaatgact atttttgata aaaaacgggt gatgacaata
    55441 tttttgtcac cacccttttg tttttcattt ggcatttgaa ggaaaatgtt aaagtaaatc
    55501 ctcttttttc gtaaatttga attttttgtt ttcttttgtt ttagggtttt cttttttgtg
    55561 gtcttggatt gcttctttcg taatcttttc ccgatttttt ttagatgaat ctaagcgttc
    55621 tgcgtatact ttttcttttg gcttatcatt cattggaaag gacctcccta cattatttca
    55681 ttctacctct agtttatcat ctgttaagga ctagtggaaa cgttttgcct aacttataag
    55741 ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa caagctacaa ttgatttatt
    55801 gaaattgttc ctgtagctca gtaggataga gcaatcgcct cctaagcgat aggtcgctgg
    55861 ttcaattcca gtcaggaacg tacaaataac ccctgtatcc tttgcggtac agggggttat
    55921 tttgtataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta
    55981 agtatattta ctccttgata aagtaaaata gatgcatgcg tatgtcttag tccatcaata
    56041 aacagtcgaa gagaaaatag ggaaaaattg attactaaag aaaaacttac taggtcgacg
    56101 gtgtagttga ttttcatact tcaatagatt cgaattaaac gatttcgatt ggcttatacc
    56161 agtcctatcc aacgaaagat atatcgttct taactctagc ggtataactt gaagctttac
    56221 gtaaagccaa tgggaatgaa ctggagatga ctttttatta tttttagtaa tagctgatgg
    56281 gttaatactt acaactttcc ttttcaaagt accatataaa aaaacggtgg ggttgtttag
    56341 cgaaataaag gatctaagcc aatagcttag atcctttatt ctatgcttta tattattttt
    56401 tgcatgtgtg cgaaagtata ctcaagccca atcgccattt cggaaaatcg gtactgtaga
    56461 gccatcttca cggattccgt cgatatccat cttgtcagat cctaccatga aatctacgtg
    56521 ggtttgactt ctattcaatc cagcttctgc taattcttca tcggacattt ctgtaccgcc
    56581 tttcacactg aaggcataag cagaacctag tgctaagtga ttcgatgcgt tttcatcaaa
    56641 taatgtattg aaaaagatga tgccagattg agaaataggt gaagggtcag gtacaagtgc
    56701 gacttctcct aaacgacgcg caccttcatc tgtatcaagc agttttgcta agacatcttc
    56761 tccttgttcg gcagaaaaat caacaacttt tccatcttta aaagtaaatt tcatacccga
    56821 gatgatggtg ccagcataac tcagtggctt tgtacttgaa atgtaaccat ctactcgatg
    56881 actgtcaggt gctgtgaaca cttcttccgt cggcatattt gccatgaatt tttctccacg
    56941 agcattatag cttccagcgc cttcccaaag atggttttta ggcaatccga taatgatatc
    57001 tgtaccggga gcagtatagt gaagggcaga aaattgttct cgattcaatt cttctgcttt
    57061 ttttgctaat ttttcatcat gcttcttcca tgctaagaca gggtcttctt catagacacg
    57121 agtcgttttg aaaatctgat cccataatgc ctcaacttgt ttttcttctg gcagttctgg
    57181 gaagactttt gctgcccatt gtttaccagc cgcagccacg acagtccaac tgactttatt
    57241 tgcttgagtt gccttgcgta aattcattag tgctttgcca ttcgctgatt gataagacgc
    57301 tactcgatgg ctatctacac cagcaaatgc atcgggatcg gcagaaacaa cgctgatcct
    57361 gcttgctcct ttttcaagcc attcatccgc ttgatcgatt ttagattgtg gtacattctc
    57421 aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg atctggtcgt cagtccattg
    57481 aacgataacc tcagcggctc ctagtttata agcttcttgt gtgatcaatc gagcaagggg
    57541 tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct ttttctgtgg ctacgccagt
    57601 ttctgcaatt aagcgtgcat atttttttag aagttcatta aaatcagata acataataaa
    57661 aattcctcct caccattttt tctggtaact attagcatga taccatttaa ttatgattca
    57721 aacaatgagg ctttaaataa aaaaaggagg ttattttttt aaggttaagt ttcatgatat
    57781 atgttactac ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt gcatatcaaa
    57841 aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt ataaaaaaat taaggagatt
    57901 aaaaaatatg gcaagaaaaa agacgattac tagagaacag attttggcag ctgcttatga
    57961 agtagctgcc accgatggat ttacacgatt tactgcgaga aacatcgcga acaaaatgaa
    58021 ttgttccacg caaccgattt atcttgagtt taaaaatatg gatgacttga gacaagctct
    58081 ttttgagcag atctatgatc atttacaata tgaagtgttc actgttgagc atacagggaa
    58141 tacgattatc gatcttgctt ttaattatat ccattttgca agaagagaga agaagttgta
    58201 tcgttcactg tacttagaag aatatggtgg aggaaaagag atgcagaatt tctcttatac
    58261 ttactttgtg aatacagtga aacgcgatcc agcttatgtg aatctttcag aagagcagat
    58321 cgaatctttg tataatggga catggatcat agctactggg atcgcagctt tgatgtcttc
    58381 aagcatcatc catccaactg atgaacagat tgaaaagatg atccaagaca gtatagatgc
    58441 tatcttgaaa ttcaaagaac caatcgaaat cgattgaata tactcaaaag ttccacaaat
    58501 cagtaggtaa caagagatgt gtcaaaataa ggaaaagttc gtttttctta cagcgtatgc
    58561 atttttaact cccgaagaac tcttccatga atgcaatgaa taaaagagcc atacagattg
    58621 aataaatctg gatggctctt ttattgcaag tatattaaat ttttgcgact agttctttag
    58681 cgaaagcttc taaattttcg atatcttctt cttcagcatt cagatccact ttaacatttt
    58741 cggctccttt tgatgcgcct gtttttgcaa aagcagcatc gaaatcatcc accgatttgc
    58801 agaattcatc gtaaaaagta tcaccagatc cgcagacacc gtaaattttt cccaatagat
    58861 ccaattcttg caagtcttca taaaagtcta cgatctcatc aggcagatcg ccatcatcgt
    58921 atgtataagt agctactacg caaatatctg cctcctcgaa ttcatctgca tctacttggg
    58981 tacattcgtt gatttcgact tcgatatcca gattttcaaa tgcttctgca acaatatcgg
    59041 cgatttcttc agtgtttcct gtcatgctag catatacgat ttttgctaaa gccatgatgt
    59101 gcctcctcgt ttataattat ttaacaatta tgaattatac caaaacatca ttgaaaagaa
    59161 aataaaacct aaagacacct ggggaatgtc atttggctaa atgtatgccg ctatgttaaa
    59221 atgagaggga tttgtaaaaa ggagacgaaa atatgattac tttaaaatca gcacgtgaaa
    59281 ttgaaatgat ggcagaatct ggagctttat tagcagatgt acataaaaat ctgcgtgatt
    59341 ttataaaacc aggaatcaca agttgggata ttgaggtatt tgtcagaaac tatatcgaga
    59401 gtcatggcgg aattgctgcg caaattggct ttgaagggta tgaatatgcg acttgtatca
    59461 gtataaatga tgaaatttgt catggattcc ctcgtaaaaa gcctttgaaa aacggtgatt
    59521 tggtaaaagt cgatatgtgt atcgatttga aaggcggcat ttctgattct tgttgggcct
    59581 atgtagttgg agaatctaca cctgagatcg accgattgat ggaagtaacg aagaaagctt
    59641 tgtacatcgg tattgagcaa gcacaagtag ggaatcggat tggtgacatc ggtcatgcta
    59701 tccaaactta tgtagaaggc gaaaatctag cgatcgttcg tgattttatt ggtcatggcg
    59761 ttggtcctac gattcatgaa gaaccagcag ttcctcatta cggagaagct ggaaaagggt
    59821 tgcggttgaa agaaggcatg gttatcacga tcgaacctat ggttaatacc ggaacttgga
    59881 aaatgaaaat ggatcctaac ggctggactg cgtatacaag agacggcgga ctttcttgcc
    59941 aatatgaaca tacattggct attactaaag atggtccaag aattttgaca tcacaaggag
    60001 aagaaggcac ttattgatct atccagtcaa taggtacggg agaggtacat gaaagtttgg
    60061 aacaaaataa aacaaaatga aaatctgatg cgctttattg agacgactca aaagcgcatc
    60121 actgattcag aaatgagtac gacctctgtc gtcgttgctt attatttact cttgtcttta
    60181 tttccattga ttattgcttt tggtaatatt ttaccgtttc tccacatcga tcaagagact
    60241 gtactgacct atttacgaga agtgattcct gagaccgttt accagtttat cggtcctgca
    60301 atcaaagacc ttttgacaca aagttcagga ggcttgttat ccgtttctgc attagcggct
    60361 ttatggtctg caagtcaaag tatcaatgct ttgcaaattg cgatgaataa agcgtatggc
    60421 gtagaaaata gaaaaaattt tttgatcgtc cgtttcttct cactaattgt cattgtcttg
    60481 ttcatgattg ctattagtgg ggtaactatc gttcttgggt tgggacagct gattttggat
    60541 gctattcagc caatcgtaca aatacccgtg aactttatcg atcagttcca aactttgaag
    60601 tggccaatca cattggtagc tttatttgtc atcatgttct tgatttattt aattgtccct
    60661 aatgctcagt tgaagttgaa gacgatcatt ccgggcacga tcttcgctac tgtcggctgg
    60721 atgctgttat cacaagtgtt tagtatctat gctaggtatt ttgcaacgag gatcagcggt
    60781 tatcagatca taggaagttt tattgtactc atgctttggc ttaattttgc tgctacgatc
    60841 atcattttgg gcggaatcat caatgcagtt gttcaagaat acgtgacggg tcatcaaatc
    60901 aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat tgaaaactaa attttctcat
    60961 aaaaaaaagt agaaacaaga gttcttattg taaatttata tcagtaattt ataataatta
    61021 tgactcttaa ttaaagtagg tgcccatatg cttgtttctt ttagtatggt tataagattt
    61081 tttctgacaa ttttattatc actgatatta acacctgtat tcaaaatcat ttcagtacaa
    61141 acaggtatgg tcgacaaacc aaatgaaaga agaataaata aagttccaat gccttcagcg
    61201 ggcggactgc ctatctttat ttcatttgtt atatcgactt tattcttatt ccgtaaaatt
    61261 atccctcaat cgtatattgt accgatcttg attgcttctt tagtaatcat agtcacagga
    61321 gtgctcgatg ataaatatga gctgacacct aaacaaaaaa gtatagggat cttgattgct
    61381 tctcttatta tttattttgt agcagatata cgtattgatt ccttcacgct tccttttatt
    61441 ggatatatcc aactaggctg gcttagtttt cccgtaacga ttttttggat cttcggtatc
    61501 acgaatgcgg tcaatctgat tgacggtttg gatgggctgg cggcagggat ttcattgatc
    61561 ggtttaatta cgataggaat cattggatat tttttcctcc atgcttctac agtgtatatt
    61621 ccagttgtta ttttctgtct tgtagcaagt attatcggtt tttttcctta caatttttat
    61681 ccggcaaaga tttatttagg cgatacagga gctctattcc tagggtttat gatggccgtc
    61741 ctttcactac aagggctgaa gaacgtgact tttgtttcat cgatttcttt gctgatcgtc
    61801 atgggagtgc cggtaacaga cacgtttttt gctatcatta ggagaaaagc aaaccgtgtt
    61861 tctttttcta cagcagataa aaaacatctt catcaccgac taatcagttt aggctttact
    61921 cataaaggtg ctgtcctgac gatttatgcg atggctttga tgttttcttt cacagctatg
    61981 gtcatgaatt atactggaag aatcggaacg atcgtattga ttattgccat gttgtttgct
    62041 gcaattttat tatttgaatt gattggtctg atcaatgaga aacatcagtt tattttaaaa
    62101 acattgcgat ttttagggaa taaagagtac cgtacgcaag taaaacagaa atatttgaag
    62161 aaatggaacc gtcatataga tgaataacaa attaaaccag ccgatcctta ttagtattgt
    62221 gacggcaaac agtgataaaa tttttcggac actagataat tttatttcaa ctgtaaaaga
    62281 tgaaacagca gtaaaaatcc gaatttttga caacaattca cttcccgagt acaaaaaaag
    62341 actaacagag tataggaaat atccctacat cgatatctat tttcatgatg aaaattccgg
    62401 atttggttat ggacataatc acaatctgat agctagcgat tctcgctatg gcattatttg
    62461 taacccagat attttagtgg atgaagagac agttacagca ttggtcttgt tattgaaaga
    62521 acatccggaa tgtgcgatgt tagcacctaa gattcttaat gaagatggaa caactcagca
    62581 cctcatccgt gagcagctgg ctgtttttga ttacgttttg cgctttgtgc ctttcaaatg
    62641 ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa tgccgaggat tgcctactga
    62701 tgctaatagt tatgtacgga tgatctcagg tagttttatg gtagctgata ttgataaatt
    62761 taaagagata aaggggtttg atgagcgttt cttcatgtac tttgaagaca acgacttgtg
    62821 tttgagtttt gaaaaagcag gttacaagtt attgtacaca cccttttatg cagttatcca
    62881 tttttacggg aagggtgcgc atcgaaatgc aaaactgttt cgcgtgtttt tgacttctat
    62941 gatgaaattt ttcaataagt gggggtggcg ttttttctga tgaaaccaga aatattagta
    63001 gtcgttgtgc tttatcagca actattcagt caatctccag cttatggacc attaagcaaa
    63061 gcacttagcg aaaagcaaat tcagcttatt atttacgata atagtcctca aaggcagcaa
    63121 aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg atccgcaaaa tcctggttta
    63181 gcggtggctt ataattttgc gttgtctcaa gcagatgaag gaacacgttg tctcgtcact
    63241 ctggatcagg atacgaggct agtcgacgat tattttgaac tattgcgtaa agtaaccttt
    63301 acagatgaat gtgttgctgc agttccgatg attttttctg ggagccgaca agtttctccc
    63361 ctatattcag atcgttatat caatcgtaca gctcaagcag tagaagttga aggcccaaca
    63421 gctgaacgaa tcatggcaat caattcaggt gttgcctggt ctgtcaaatt tctaaaaaag
    63481 attggcgggt ttaatcctgc tttttcttta gattttttgg atcattggct gttttggaaa
    63541 gtggatcagt tggaaaaaac tgtggagatc cttcctgtac gaatggaaca tgatctttcg
    63601 gttttagact atgaaaaagt ttcaagtaat cgataccgtt ctattttaca agcagaaaat
    63661 cgattttatc atgaatatga tcaaaggcat ttatccagtc accgacgaca attattgtta
    63721 agaacagcta aacagttctt gaccgtcaaa aaccggcata tctggcggat gactttgcgc
    63781 tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg gtttgtatag ctacctttaa
    63841 tggaggcaga tatttaagag agcagttaga tagtatttta tctcagcttt cagcagaaga
    63901 tgaagtagtc atttctgatg atgggtcagt agatgataca tgggatatat taactgaata
    63961 cgcagaaaaa gataagcgaa tccagctttt ttcggggcct cgtcaaggat tgatcgctaa
    64021 ttttggttat gcgatccagc agacgaaagg agatctaatc tttttggcag accaagatga
    64081 tgtttggttg gagacaaaag tgaaaaagat cactgactat tttgagcaga atccggatca
    64141 gctagtagta gtttctgatt taatcattgt ggacagcgag ctgcaagaga tccatgattc
    64201 gtatttttcg tttcgaaatt ccaaagaagg atggcttaat aatgtgataa gaagtcacta
    64261 tattggtgct ggtatggctt tccggtcttc tttaaaaccc gatattttgc cgatcccgtc
    64321 agaagtgccg atgcatgata tgtggattgg tttgctggga ggaaagaaga ctgggttcct
    64381 aagagaaccg ttaactctgt accgaagaca tgatttaaac gcgagtgaga ttgaaacaca
    64441 ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg attacggcgt tgatcaaacg
    64501 aaaatggttt aaacgttgat aaaagatgtt taaatcctta atatttccat gacaaatata
    64561 acaattgatg agataataac aaagtgaaat ttgaaatcaa aaaggagagc agatatccat
    64621 gaagggaatc attcttgctg ggggcagcgg aacacgtctg taccctttaa caaaggcaac
    64681 atcgaaacaa ttgatgccga tttatgacaa accaatgatt tattatccaa tgtctactct
    64741 gatgttggcg ggaatcaaag aaattctgat tatttcaacg ccacaagata caccacgttt
    64801 caaggaatta tttggaaacg gaaatgattt aggactgcag atcgaatatg cagtacaaga
    64861 aagtccagat ggattggcac aagcgtttat cattggagaa gaatttattg gagatgatag
    64921 tgtctgcttg gtattagggg acaatattta ctatggtggc ggtctgtcta aaatgttgca
    64981 acgagcagct tcaaaagata caggcgcgac agtatttggc tatcatgtca atgatccaga
    65041 acgctttggt gtagtagaat ttgatgagaa tatgcaagct ttgtcgattg aagaaaagcc
    65101 tgctcagcca aaatcaaatt acgctgtaac tggcctttat ttttatgaca atgaggtaat
    65161 ttctatcgct aaaggtatca aaccttcaga acgtggagaa ctagagatta cggatgtcaa
    65221 taaagcatat ttggataaag gaaaactatc cgtagaactt atgggacgcg gatttgcttg
    65281 gcttgataca ggaacacatg aatcattgct tgaagcatct acctttatcg aaacaattga
    65341 aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt gcttaccgta tgggctacat
    65401 cgataaggaa caattgctgg cccttgcaca gccattgaaa aaaaatcaat acggtcaata
    65461 tttattgaat ttggccgctg aataaggagt ccttacagat gaaagtaaca gatacaaaat
    65521 taacagatgt caaaatcgta gaaatggacg tctttggcga tcatcgcggt ttcttcacag
    65581 aaagttattc aaaagcaaaa tttgcagaac atggattgaa atatgacttt gttcaagaca
    65641 accattcact atcagctgaa gctggtgtcc ttcgggggct tcatttccaa aaaggagaag
    65701 ctgcacagac aaaattgatt cgggtagtca ctggagcagt tttagatgta gtagtggata
    65761 tccgcaaagg tagcccgact tatggtcaat gggacggatt tatcttatct gaacataatc
    65821 atcgacagtt gcttgtacct aaaggatatg cccatggatt cgttactttg acaccaaatg
    65881 tgaacttcct ttataagtgt gataattact ataatgcaga agcagatggg ggcatcgcat
    65941 ttgatgatcc agatttagcg attgattggc caattgatcc atcaagagcg attatgtcgg
    66001 aaaaagatca ccgtcatccg acattaaaag aatttgaagc agaaaatcca tttgtttacg
    66061 gagaaatcta agaattaagg agaaattttc ttatgaaaaa catcattgtt accggaggtg
    66121 ccggattcat cggctctaat ttcgttcatt acgtggtaaa caatcatcca gaagtgcatg
    66181 tcactgtttt ggataaactg acttatgcag gaaacaaaga gaacttggcc ggacttccta
    66241 gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc agaattagtg gaccgtctag
    66301 taaaagaagc ggacgcagtt gttcattacg cggcagaatc ccataacgat aattcattaa
    66361 aagatccttt cccatttgta caaacgaata tcattggtac gtatacactt ttagaagctt
    66421 gccgcaagta tgatgtacgc tatcatcacg tatctactga cgaggtttac ggcgatcttc
    66481 cattacgtga agacctgcca ggacatgggg aaggcgaagg agaaaaattc actgcggaaa
    66541 caccatataa tccttcaagt ccatattctt ctacaaaagc tggttcagat ctgttagtaa
    66601 aagcatgggt gcgttctttt ggcttacgtg caaccatttc aaactgttca aacaactatg
    66661 gtccttatca gcatattgaa aaatttattc ctcgccaagt aacgagtatc ttaagcggta
    66721 ttcgtccaaa attatatggc gaaggaaaga atgttcgtga ctggatccat acgaatgatc
    66781 attcttcagc agtgtggctg attttgacga aaggcagaat tggcgaaacc tatctgatcg
    66841 gtgcagacgg tgaagaagat aataagaccg tgatggaatt gatcttagaa atgatgggcc
    66901 aaccaaaaga cgcatacgac catgtaaacg accgcgcagg gcacgatttg cgttatgcaa
    66961 tcgattcgac aaaattgcga gaagagctag gttggcagcc tgagtttacg aacttccgtg
    67021 atgggttggc tgacacgatc aaatggtacg aagaacatga agactggtgg aaaaaagaaa
    67081 aagaggccgt tgaagcagca tatgccaaaa atggacaata atacagagga aatttcactg
    67141 tattaatgga aaaacacatg tgaggcaggc gctcgcctgt ctctttttta aaataaaaat
    67201 caaggagcgg ataaccatat gtttttatta actggaggaa atggacaatt aggtacagaa
    67261 ttgcgccatt tattagatga aaaaaatctg ccatatgttt caacagatgc aaaagacttg
    67321 gatatcacag atgctgagaa aacaatggct tatattacag aattaaaacc cgatgttatt
    67381 ttccattgcg cagcttacac agcagtagat aaagcagaag aagaagcaaa agaattagat
    67441 gagaaaatca atgtcgatgg cacaagaaat gtagcacttg cagcaaaagc tgtgaatgca
    67501 caattcgtct atatcagtac agactatgtt ttcgatggaa aaaacaaaaa tgtagaatac
    67561 gaagtagacg accaaacgaa tccactaaat gaatatggtc gtacgaaatt attaggcgaa
    67621 aaagcagtag aagagatttt agaagactat tatatcatac gaacttcttg ggttttcggg
    67681 atctatggac acaatttcgt cttcacgatg caacgcctag cggaaacccg cgacaaactg
    67741 actgtagtaa atgaccagtt tgggcgacca acttggacac gtactttggc agaatttatg
    67801 gtatatgtca tcgaagaaaa agcaccattt ggtatttatc atttgtcgaa cgaaaatagc
    67861 tgttcttggt atgagttcgc aaaagaaata ttgaaagata cagatgtaga agttgcacct
    67921 gttacgtcag aagaatttcc gcaaaaagca acacgtccgc agtattcagt catgagcctg
    67981 aaaaagacag aagcgttagg ttttgtcata ccaacttggc aagaagcact tgcgcaaatg
    68041 ctggaagctg ccaaaaaaat gaataagtaa tttccttttt atcaaataga atttggagca
    68101 tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa aagccaaaag cgaatcaggc
    68161 agattcgaat ttggtgcttg acctgtttct aaaactccgc tatcttattg gcgttttagt
    68221 catcgtggtc atcgttactt ttaatttaaa cggaagttca cttggtgttt gggataaata
    68281 tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt tttggacaga atcgggaagt
    68341 gcggtcagac gaatggctgg tgcagacacc tttttacctc tcacaagctg aagaggactt
    68401 ttcgttggtg aacgaggatt attcattaaa cggacaaaac atgatcattg cgtataattc
    68461 tcctgtaaag gatattacgg ttatcggaaa accattcaac tggggattct ttttcctcgg
    68521 aagagatcga ggactttcct tttattgggc gatgaaatta gtcgtcatgg tcttgcttgg
    68581 attcgaagta ctgatgatat taacgaagag gaaaaaagct ctatcgctaa tcggtgcatt
    68641 tgcgctgaca ttttctccag cagtccagtg gtggttcatg caacatgtgg gcgatttgat
    68701 tttctttact ttgggactga tgattgcttt ttatcattat ttctacaatc atgataaaaa
    68761 atggctacga acgttgatga tgcttttggt ggttattttc ggtattgggt ttattttagt
    68821 gatttaccca gcccatcaag tgatgctggc gtacttgctg attttttatt ttgttggcct
    68881 gcttatttac tatagcaaga aagtttcatg ggatctgttg gatgtagtgt tgattggagg
    68941 ggcagtttta tttattggcg gagtcatggt tcatttctgg ctaacgtcaa aagacgcatt
    69001 gttagcttct ttgaatacct tatatccagg aaaacgggta agtactggag gaaagtggac
    69061 gattggcgaa tttttttctt tccttacaaa ttggaaaatt ccttttgaag atatcaacta
    69121 ttcgaataac tctgaagtgg ccctgttcta tcactttttt ccaactgtat tcctagcaag
    69181 tccatttgtg ttatttggca aaaaagatag cgaacaaaag ttactgggac gtattttgat
    69241 gattttttgt ctgttttcta tcttttggat taccgttggg ttgccaaaat ggctagcaga
    69301 aatcactctg ctgtcttatg ttcctccatt aagagccatc cttactttta gttttgcggc
    69361 ttgtttatta tcgatctggt ttatcgctta tatttggaga gaagatgtta ttcctatttg
    69421 gtataaattc attttgttgg cagtaaatgc atggtcttat ttctatgcga ttacgcattc
    69481 gaaaatggat aattattttt cggattttga aaccatcgca gttgctgtgt tgggaacagt
    69541 gattttagct tttgcattat ttggctggag acgcttgttt tacctcgctt ttgtcgcctt
    69601 agttatcgta tctggctttt tcgtcaatcc agtcgtagaa ggtacaggag cgatttttga
    69661 aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa gatcctggta aagtttggtt
    69721 gtctgaagat gaattatata acttcacccc aacattggga gtgaaatcct tcaatacagt
    69781 acgcttttat ccagatatag ctgcatggaa gaaaatcgat ccgaaagaag aatacgagaa
    69841 gtattataat cggtatgctc atacaagagc ttttatcgct aaggaagata caaagttcat
    69901 tttagatcgt ccagatatgt tttcggtcac gttgaatttt gaagacgcag aaaaattagg
    69961 gatcgactat gtgatcagta aacgaccatt aactgattac aaccaattat atgaagcgca
    70021 gttcacgcag ctatatggac cggataaaga cggttatatg atttttaaag tagaatatcc
    70081 ggattatcca gatgtaccac aaacacaaac tcaggaaaat cagctagtcg tacagtaata
    70141 ggctagctga gaataatcct gaaatattag tagaaaggag agtgaagata attcaagcaa
    70201 tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt gcttttgttg ctgacgctag
    70261 tcttttttgt ttggacagca ggggtaggtt ttccagcaac tttgtttata tataacaata
    70321 gttcattact tatcgctttt agtgtggcta ttttgctcat tttaaatgtt tataaagcaa
    70381 aaggcgtcga ttttctttat ctaggtgttg cgcttgccct tttcattttc ttttcttact
    70441 ttcgggaatt aagaaatgag tctttatttg gagacttact gtttcctttg gttattttat
    70501 tttgtttagt aattaaatgg atcgagtgga caaaatggga tcgcggtatt tatctgattg
    70561 tctttacggt cttcctcgga atcacgttat atcgtgtatt tacagagatc caagtaccag
    70621 aaggacaaag catctgggct ccaggcaatt cgctttctga tatttggatc aatacgaata
    70681 caatcggaag ttctttgatg actcttgctt tattgatcag tggatttgcc agttcttttg
    70741 aacgatggta tgttcgaatc atcggtggga tagcagtcgt tgcagccttt ttaggtattt
    70801 gggtctgcca atctagaggt gcattagtcg cgttgattgt ttttgcgata ttggatcttt
    70861 tacctaaatc gttgatgcgt gtcattcgtg cgccatttat tgcctacacg gttacgattc
    70921 ttttagcttt gccgatttct tatctggcgg ctgtctctga aaaagtcaat ttgtttaccg
    70981 atcgagaaga tatttggcat aaattctatc agacgctagg agaaaaatct gaacaagttt
    71041 tactaggaat gaaaacattt atcttccagc gaggaaacca atttttagga aaccacaata
    71101 gttataattc tattttgaat atctatggat tgataggatt tgggattgct gctttgctgc
    71161 ttatcctttt tattggtcgc ttaacgttga aagcagattt atccaacggg cagatgactt
    71221 ttatctgggc cttttttgcg gtgatgatgc agtcatttat ggaggataca ttgacttcat
    71281 acccatgggt tccgattatt tatatcctgt tggctatggc cgttcatcga tatgatgaac
    71341 cagaagttgc ggaggaattc tatgttatgg aagaggcata tgacgatatg acttatgatg
    71401 atacatctta tgaggaacaa ccagatagcg ctcattcttc caggatccaa cgctatcatt
    71461 gataggcaca aaatacgaga gaggctgtga caaccgtcgc agcctttttt ggtttattgc
    71521 aattgtctag catcttctgt aaaatagaaa cgttagagaa aatcttgtat catagaaaat
    71581 taagaaagaa ataagaacgc agctcggtac caaaaatatg gtaaaatggc acttgactgt
    71641 aattcactga aaatcgcttg gaggaacaca aatgtttaaa gatttgtatt tatttatgaa
    71701 ggacatttat cagaacagaa aattattgct acagttttcg atcaatgact tcaaatcaag
    71761 atatgcagga tcgtttttag gaatcttatg ggcgtttatc aatccgctat ttactgtatt
    71821 gatttattgg ctggtatttg gtgttggact gaaagcacaa atgatcgatg caaaataccc
    71881 atttatcgta tatttagtta ccgggatgat tccctggttc ttcttctcag atgcatttgc
    71941 atcaactacg gttgtttttc gggaatatac ttatttagta aagaaagtcg tattcaatat
    72001 tcgtatcttg cccacgacga aaatcctatc caatctgtac acccatttat tcttcattct
    72061 gattggcata gctgtcgcgc ttataaatgg tatatatcct tcagcaatgt ctttacagct
    72121 gatttattat ttattttgca tgtgtgcttt cttgacagga ttgacttggc tgacggcatc
    72181 gatccaacca tttttacctg atatcatgca attaattacg atcgtgctgc agttgatcat
    72241 gtggacatta ccgatattat ggagccctaa tcagtttgat tcaggtcttg taagcatatt
    72301 gaaattaaat ccgctgtact atgttgttca aggttatcgt gagtcatttt taagtgaagc
    72361 ttggttctgg gagcatggaa catatacttt gtatttctgg gttttcacgt tagtaatgtt
    72421 cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat ttttcagatg tgctgtaagg
    72481 agaatagcta tgacagaata tgcaattaga ctaaaagata tcacaaaatc ttataatatg
    72541 tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc cttttaagaa atcctatcat
    72601 gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa aaaaaggtga gatgatcggt
    72661 ttcgtgggag aaaacggatc gggaaaatct acaatgttga aaatcattac gggagtactg
    72721 acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct cagctttgct agaactagga
    72781 tcaggattca atcctgagta ttctggctat gaaaatatct atttaaatgg aatggttcta
    72841 ggattttcaa gagaagaagt cgatgaaatg gtcgatgata tcattgaatt tgctgacatc
    72901 ggagaccata tttatcaacc tgtaaaaact tattcaagcg ggatgttcgt tcgtctagct
    72961 tttgctgtag caatcaatgt gaatcccgat attttgattg tggatgaagc attggcagta
    73021 ggcgatcttg aatttcaact aaaatgtatg gaaaaattta cagaaatcaa gaactcggga
    73081 aagacgattt tattcgtttc tcatgatatc agttcgattc gtcgtttttg tgatcgttcc
    73141 tattggttga aaaacggaga agtcgtcgaa tatggcgata cgatggaagt aacgacgaat
    73201 tatgaaaact ttttaaagaa aaaatcagtt aaaacagtgg atcggaataa aaaccaagta
    73261 gaacggttcg ccgctccaga tatcgtcgac gtgacgaaag cggaattgtt ggataaagac
    73321 aaaaatccgg tggatatgct ggaacaagga gaaaaactat acgttaaagt aacttatgaa
    73381 gtaaaagatg atacaatcaa aaatccggtt ttaggaattg cgctacgaac agtcgacaat
    73441 ttttatgtat gcggacttaa tactctacta gatggtatca agattccttg gaaaaaagga
    73501 gagaatatct tttatctgga gtatgaaaag ttaggtttgt tagctggtga atattatttt
    73561 gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg tatataagac aaagtacatg
    73621 aacttgtttg tcagcggttc ttatatcgga gaaggcatcg tggtcttaga ccataaatgg
    73681 gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg gacctagatg aacaaagcag
    73741 tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa gttttagagt ttggtcccgg
    73801 aaacggccga ttaacgaaac atttaatcgg agcaaaacaa tgtgaagtga gcattgttga
    73861 gttagataag gaattgttcg attttgtcag cgaatttgct caagatggat tttatggtga
    73921 tatcgaaagt tttgagtggg caaactatta tgctggacag acatttgatt acgtcttgtt
    73981 tgcggatgtt ttagaacatt tggtagatcc tggaaaaaca ttgaagaaag tcagagaatt
    74041 tttgaatgaa gaaggagaga tcctgattac attccctaac ttggcgcata attctgtcat
    74101 gatcgatttg ttcaataacc aattgccgtg ggcttcttat ggtctcctag atgaaaccca
    74161 taattctttt tatactcatg atggcttcca aaaagttttt gaaaaagcgg gattattcat
    74221 taatattgaa gattatttgt acttagctgt aggtgataca gaattaaaat caacttatga
    74281 agaattgccg gaagctgtac gctatgattt caaaatgcga ccatttggtg aagtctacca
    74341 atactttttc tcattgatga aacatccagt agcacaaagt tccattgcag agccacaaaa
    74401 ttctaattat gtaaaagtgt tagaagtaac ccagcaaacg aaacaagatg aaacaatcca
    74461 acaaatacca ttcaataatt tcacagggga aaatgagaca ctaagtttcc ccgtatcaga
    74521 aacaacagaa agaatggtgt ttcgcttcgc gcaacaacct agctttatcg aattcagcgc
    74581 agaagctgcc ggagaaaaac taacatttat cgattcgaat gctgtagtca aaacagtaaa
    74641 tgattgttat ttgtttgatg ggaaagaatt accagagttc gttctaacgg atatttctgg
    74701 gaaagaagtg actatccatt gtcactatcg ctttatcggg gaattgacac caacaatgaa
    74761 agaattgttg gaaacgattc gtcccatggc tgaagtcaca aagcagcttt ctgagaaaaa
    74821 tgacgaatta gaaagagaaa atcatcatct tcttgaggaa aatacaagac tagaccatac
    74881 gttaaaaaca accacaaatc gctattgcac attgttggaa agtgatgaat ggacaataaa
    74941 acatagacta ggacgacgta agaaagaaac ttcgaaaaaa atccaagaaa aagaactttc
    75001 tatatgtatt gacgagaaaa tctgggatgc agaaacaaaa atattaaaaa tcatcggatg
    75061 gggaatcgct aattcggatc gccagccctt gtcctataag ttatcagctg accaatcgcc
    75121 atttttcgaa gcaattcctg tttcacgaga agaagtcaat caagcagaac agctcgcaaa
    75181 aggaacagaa gcgggatttg agttacgaat cctttgcgag caagaacgtt cattcttagt
    75241 ggaagcagta gctcaaaatg gccaatcatg gatcattgaa atgtaaataa tgattaactt
    75301 ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa ttgccgtatt tatcgatcaa
    75361 atcactcgtg aacgggccac tggcgatttg cttattgtgg gctgggcaat tgatgaagtg
    75421 accaaagaaa tccctacgat aaaggtagaa aaagaaaatg tgattgctga agcaacacat
    75481 gtcgtaagat tggatatcaa tcatttatat aatcttgatg ttaagaccca aagcggtttc
    75541 aaggtacgtt tatcagggaa aatgagagga aaagccatct tagacttcca aacagcgaga
    75601 catcaaaatg gcatagctgt gaaattaaac ggtaaatacc cttatgatga cggcattgaa
    75661 tcttcatggg aaagaaaaaa aagattactg aaaaaaggga taaattatgc aaggactcat
    75721 ggcgtaaaaa aagcgatccg tcgggtcaaa ttggaattga agccaggatc gatcgactat
    75781 gcagagtgga tcagtcgtca tgagaaacca gatttgaaag agcaaaggaa agaagcacaa
    75841 ggatttgcgt accgtccatt gatttccata cttatgcctg tctataacgt tgaaatcaaa
    75901 tggctcgaaa aatgcattga ttcggtactg gatcaaacgt atgatcattg ggaactatgt
    75961 atcagtgatg atgcttctac tgatccagct atccgaaagt gtctggaatc ctatcaagca
    76021 aaagatgata gaatcaaagt cgttttccga aaagaaaatg gacacatcag tctggcaact
    76081 aattctgcat tggaaatagc agaaggagaa tttatagcac tcttagataa tgatgatgaa
    76141 ttaccgccat ttgcattata tgaagtggca aaagtgctaa acgtacatcc agaactggat
    76201 gtgatctaca gtgacgaaga taaaatcgat gcagatggca atcgctttga tccgcatttc
    76261 aaagcagact ggtcgcctga tacattgatg ggaaataatt atatctctca cttaggcgta
    76321 tatcgaacaa gcattgttaa agaacttggc ggtttccgta aaggctatga aggttctcaa
    76381 gattatgatt tggtccttcg tgtgacagaa caaatccctg cagatcatat ttaccacatt
    76441 gaccgagtct tgtatcattg gcggacgatc ccaggatcaa ctgcaagtaa tggcgaagcc
    76501 aaaagttata tctatgattc cggagtgaaa gcattgacag atgccctgtc tagaagaaac
    76561 atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt atgagattgc ttatgacgtc
    76621 ttgcaagagg atttggtcag tgtgatcatt ccaacgaaga atggctatga agatctaaaa
    76681 acttgtgtgg attcgatcat cgaaaaaacg tcttattcaa attatgaaat cattattgcg
    76741 gataacggaa gtacagatcc aaaaatgcaa gaattatttg cagaatacaa gcatcagctg
    76801 aaagaccgct ttatcgtaga attgattgac attccattca attattctcg aattaacaat
    76861 ttagcagcag aaaaagcaaa cggcaaatac tttttattct tgaataacga tacagaagtg
    76921 attgagccag attggatgac ggtgatggtt tcttatgcac agtttgatcg aatcggctgt
    76981 gtaggagcaa aactgtttta tccagatgat acaacgcagc atgcaggagt attattagga
    77041 atcggtggtg ttgcagggca tgcattgaat aactatgatc gaacacattg tggttatttc
    77101 ggccgattgg tcattgacgt caattactta gcagtcactg cagcttgtat gatggtcaag
    77161 gcagctgact tcaatgctgt taacgggttt gatgaaacat tagaagtagc attcaacgat
    77221 gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca atgtctatgc acatcaagca
    77281 gaattgtacc atttcgaatc taaatcaaga ggttatgaag atactccaga aaaacaaaga
    77341 cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc cagcatatat tgcgcatgat
    77401 ccattttaca atgataattt aacaaaagac ggaatcggcg acttttcgtt gagaccagat
    77461 taaactataa taaaaagctg ttatgttgtg attcgctcgc acataacggc tttttatagt
    77521 tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa taagtcgagg ttgggacaga
    77581 agtgtttaac tccgagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga
    77641 atttcggctt atttaagaag aagttgcttc tgctcccacc gtttattcgt ttttaaagaa
    77701 tgagacaaaa gtgtttctta cttttgtccc acgctcttat tcatgctcag agcgccaata
    77761 gatacaaaat catagccaaa gtttcacgaa ccctagctgt caaagaatcg atattttatt
    77821 ttaatatagg ctattttaaa ataaatggtt taaggtattc ctctacaaaa gccactctat
    77881 caattatcat gggtttaaaa taagaagtga tggagataac cagattgttc ttagcgtcaa
    77941 tataaagaac atttccacta ttaccaattg ctgaatatat ttcgttctca gaatctaata
    78001 cccaccataa atagccatat ctcatatttt gtaagtcttc taaagcactg gttctaggtt
    78061 tgaacatctc gtttatccac atgggtggta cgatttgttt tcctttatac atccctttat
    78121 ttaggcataa tacaccaatt tttgccagat cacttgccga taaacacaaa ccatatcctg
    78181 ccgtcccgat atttagtgga tcacaaaacc agacattggt tttaggagac ttatccattg
    78241 taaactgctt atgttcttca gctgtttcag cataataatt gacatggttt ggtatcccta
    78301 taggatcaaa tagatactta ttggcaaaat caacagttgt tagcttactt gtctgataca
    78361 acaagccagt taatatgtgt aaacagacag tttgatagtt gaattcgccg gttatacctt
    78421 ttcttcctcc caataaatct agtgatgttt ttgtccaatc ttcacttgaa cagacttttg
    78481 tccatggatc gcctttacat ttgtagggtg ctttcattgt tagcaagtgc ttgattgtaa
    78541 tagcgtaaat agttttctcc ccgcgtttca ctttatactc tggaaaataa tctaatactt
    78601 tatcatcgat acttttgatt aatccttgct ctaaagcaat gccaatcagc aaagatacaa
    78661 tacttttcgt tgctgacatg atatgacaag tatcgtcttt tttataacca ttccattcat
    78721 ctgtataaac ttctacccca tccttatatg cgacaatttg acatatattt ggttgtcgaa
    78781 aaccaataaa atcatgcaac tcttgtttat tcataagata ttacctcact ttcttctata
    78841 agcataatgt cattaaaaat cgatttcaat tttttcgatt cttcacgtaa tacaggtatt
    78901 cttatggact agacagcgct ttgggaagat ggattctctg aaaaatataa aggttcactt
    78961 tatgagtgga taaaaaaagc agcaaatact tcgacttctt taatttacca acattagtga
    79021 aaatcgattc atctggagat ttgtaattta gagtccttgt cttgattaga ggtgtataaa
    79081 gctttttatt agacattttt aaattccatt acaatttgct tataaactag gtttttaaag
    79141 gtgactatgg taagataata gagatattat tttgtgatta ggagtgtata tatgaataaa
    79201 aatggagaat ggagtgctgc acgacggata ttcattatat taatggatgt tattgtatac
    79261 aatgcagcaa tatatagtag tttcttactg aagtttcgtg gagagatccc attacgaaat
    79321 ttagaaactt tccaacactc agcaattatt atttcagtga tttttattgc gttgaacatt
    79381 ttgttgggtg catatgtttt ttataatcga atgatcagtg acatcgtgtt tgtcacagtc
    79441 atcggtcagg tattgatgac actagggatc atggtagtga cttttgcggg ccgctggttt
    79501 gcttttccac gtcttgttat cttactttca tttgttctcg gtacgctttt cttaagtatc
    79561 tatcgtattc tcgtatataa actttattta cgcgtaagtc gagataaaaa ggtcgtcatt
    79621 attgggttag aaaaggatgt cgcaccagca atccaaaatt ttggttcaaa gaaaaacaac
    79681 aaacataaag tcgaagcagt cattcttgat aactattatg caaacatcaa aaatatgatt
    79741 aaagacatcg atattgtcta tcttgcttct catatcgaag aaactgaaaa aatcaaaatc
    79801 tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga atacaacatt tgaaaacttg
    79861 acattgatca atccaaatat tatgaatttc gaagatgaaa gtattattga agcatctgga
    79921 tttagaatcc aacctgaata tgaactattc aaacgtttgt ttgatatcat cgtttcactt
    79981 gttttattag ttattgcttc accatttatg ttattcacag caattttagt caaagtgaca
    80041 tcaccgggac caatcatcta taaacaagtg cggatcacaa aaaatcaaaa ggaattttca
    80101 atctataaat tccgctcgat gaccgccaca gccgaagcaa aatctggacc agttttagct
    80161 aagagcaatg atgcacgagt aacgcctgta ggtaaattta ttcgagctgt ccgctttgat
    80221 gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt cgatcgtagg accacgtcct
    80281 gaacgttctt tcttcgtcga tcagttcaat gcagaaaacc cttattacta cttacggcat
    80341 aatgttcgtg ctggtatcac tgggtatgca caagtctatg gaaaatatgt ctctgattac
    80401 aacagtaaat tacgttttga tttactttat atcaaaaaat attcattgat gcttgattta
    80461 aagatcttgc tgcaaaccat caagatccta tttgataaaa tgtcctctca aggacttgaa
    80521 gaagacgaaa atgtgatctc attggcagaa attgcttttc cgaatgaaaa aatctttcgc
    80581 tagttgggag gcgtaaaaat gaacaatccg aaattcagca taatcgtacc agtatacaat
    80641 gcagaagcaa ctattggtcg aacgattgat attctaaaag aattggatta tgaagagtac
    80701 gaagtcgttt tagtaaatga tggatcaaca gatcaaacac aggaaatcat tcaagaactg
    80761 attgaaaatg atgaacgttt tgaattgatt actacgacca atcaagggcc cggattagca
    80821 agaaacgaag gaataagaca gtcaaaagga gaatatctct tattcttcga tgcagatgac
    80881 acgccagtaa aaacgatctt gtcagattat gatcatttgc tagaaaagag tccaaaagcg
    80941 gatcttgttg ttagttcgtt tgtttttcgt acaatggacg agggaagaat cgtttccgaa
    81001 aaagaaaact tagttacaga atgtcactat ctaactcacg aagagtttat aaaagacatg
    81061 tatcagttaa tgaatgaaca gttgatgtat gttgtatgga acaaatgcta tcgtagcgat
    81121 attctgaaaa ataaccaaat tctatttaaa acttataata gttgtgagga tcgaatattt
    81181 aacttacatt attatgaata ctgtcatcaa gtgatcatga acccaaaagt agaatatatt
    81241 tatgaatttg aaggtgggaa ggggatcact aatcaatacc gacctaacaa gttcgaaaca
    81301 tttaaagaat tctattcatt agcaaatact gtaacagacg atattaataa agacggtatg
    81361 gcggcgcttc ttttaaaagg gacaacttca gttatttttt ctatttatag tacctctgca
    81421 agaacttcaa aagagaagaa agacgaagca aagcagatat tgagtgatcc agtgatccga
    81481 gaagccaagc aaattgctaa gacggattct tcagcaaaaa aaataacaaa gttgctttac
    81541 aatatgccag ctccactatt tttatctgct gtaaaagttg gaagcttcgt tgaagtgaaa
    81601 atgccagggt taatggctgc tttgaagcga gtatattagt taatggagga aaatataatg
    81661 aaaagaattt tgcattttca gggacgaatg ggtttaggtg gagcagagtc tttcatgatg
    81721 aatttatatc gtaaaattga tcgtacaaac tatcaatttg attttttgat ttatgaagat
    81781 tatgcagatg ttcaagacta tcattcagag attgaaagat taggaggccg tatttttgtt
    81841 gtacctaatc ctaagaaaaa tatttttaaa tatttaatag aagtgaataa gttactaaaa
    81901 aaagaatctt tttctatagt tcacaatcag gtttattttg gcgggggaat taatttatgg
    81961 ttagccaaaa agaatggaat cagacagaga attgctcata gccatgcaac tgaagatgga
    82021 aaatctcaaa atattgtaat gaatgtattg cgtaaatttt taaccaaatt gttgttacaa
    82081 aatgcaacag actaccttgc cgttagtcaa gaagctggtg aaagtctgtt tcaaaaccat
    82141 ccctttgaga tagttcataa tggtattgat ttagagcttt atagtaaaaa ttcagaagct
    82201 aaggttaata agagaaaaga attggatatc tcaatgagta cttttgttgt gggaaacatt
    82261 ggacgattag agaaacaaaa aaatcaatct tatttgttag agattttttt agaaatacta
    82321 aaaatgaatt ctgattctta cttgatttta gtaggtgaag ggtcacttag aaataaactg
    82381 aaagccaaag cagtagaatt ggatatagag aaaagagtaa aatttttagg agaaagaaac
    82441 gatattcccg agttattatc tgtgatggat gtttttgtaa tgccttctct ttatgaaggt
    82501 ctgccaatgg ttggattaga agcacaagca gcaaaattaa aattagtctt agcagatact
    82561 atttccaaag atacaaagtt aacaaataat gtggattttg tatcattaga tgaaacacca
    82621 caagaatggg caaaaaaagt aataaaagag ccgtatgcta ataaaataac acaagaacta
    82681 aagagttatg atgtttcgta cactactgaa caaatggaga gtatatatag tagagatcat
    82741 taaaaatgtt caagatattt taggagtgat tatttttgaa aatatgttat ttgatattgg
    82801 catacaatga tattggcaac ttaaagagat tggtcaatcg attgaacgaa aatgcagttt
    82861 ttattattca tatagataaa aaaactgata tagaaccatt tattgaagca tttaaatcat
    82921 atgataatgt tgaattttta tgtaataaaa accgtgttaa aatttattgg ggtggttttt
    82981 ccattgtaca agcagaaatc aatttagtaa agagagcatt acaaaatgag aaatattt
//
LOCUS       DAJSBC010000004        72629 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSBC010000004 DAJSBC010000000
VERSION     DAJSBC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.004"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..791)
                     /locus_tag="ONT65_000249"
     CDS             complement(<1..791)
                     /locus_tag="ONT65_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2327908.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGK"
     gene            966..1490
                     /locus_tag="ONT65_000250"
     CDS             966..1490
                     /locus_tag="ONT65_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR2327909.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            1506..3005
                     /locus_tag="ONT65_000251"
     CDS             1506..3005
                     /locus_tag="ONT65_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR2327910.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3093..3695)
                     /locus_tag="ONT65_000252"
     CDS             complement(3093..3695)
                     /locus_tag="ONT65_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2327911.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            3799..4755
                     /locus_tag="ONT65_000253"
     CDS             3799..4755
                     /locus_tag="ONT65_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR2327912.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(5060..6016)
                     /locus_tag="ONT65_000254"
     CDS             complement(5060..6016)
                     /locus_tag="ONT65_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2327913.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            6190..6750
                     /locus_tag="ONT65_000255"
     CDS             6190..6750
                     /locus_tag="ONT65_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327914.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            6743..7567
                     /locus_tag="ONT65_000256"
     CDS             6743..7567
                     /locus_tag="ONT65_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR2327915.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            7577..8194
                     /locus_tag="ONT65_000257"
     CDS             7577..8194
                     /locus_tag="ONT65_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2327916.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            8212..8895
                     /locus_tag="ONT65_000258"
     CDS             8212..8895
                     /locus_tag="ONT65_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR2327917.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            9578..10813
                     /locus_tag="ONT65_000259"
                     /pseudo
     CDS             9578..10813
                     /locus_tag="ONT65_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            10821..11708
                     /locus_tag="ONT65_000260"
     CDS             10821..11708
                     /locus_tag="ONT65_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2327918.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            11725..12561
                     /locus_tag="ONT65_000261"
     CDS             11725..12561
                     /locus_tag="ONT65_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2327919.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            12564..13187
                     /locus_tag="ONT65_000262"
     CDS             12564..13187
                     /locus_tag="ONT65_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327920.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            13184..14476
                     /locus_tag="ONT65_000263"
     CDS             13184..14476
                     /locus_tag="ONT65_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2327921.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      14835..14932
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15048..15629
                     /locus_tag="ONT65_000264"
     CDS             15048..15629
                     /locus_tag="ONT65_000264"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR2327922.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            15632..16939
                     /locus_tag="ONT65_000265"
     CDS             15632..16939
                     /locus_tag="ONT65_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR2327923.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            17232..17720
                     /gene="purE"
                     /locus_tag="ONT65_000266"
     CDS             17232..17720
                     /gene="purE"
                     /locus_tag="ONT65_000266"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR2327924.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            17713..18837
                     /gene="purK"
                     /locus_tag="ONT65_000267"
     CDS             17713..18837
                     /gene="purK"
                     /locus_tag="ONT65_000267"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR2327925.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            18850..20145
                     /gene="purB"
                     /locus_tag="ONT65_000268"
     CDS             18850..20145
                     /gene="purB"
                     /locus_tag="ONT65_000268"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR2327926.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(20325..20702)
                     /locus_tag="ONT65_000269"
     CDS             complement(20325..20702)
                     /locus_tag="ONT65_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327927.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            20958..21686
                     /locus_tag="ONT65_000270"
     CDS             20958..21686
                     /locus_tag="ONT65_000270"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR2327928.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            21686..21940
                     /gene="purS"
                     /locus_tag="ONT65_000271"
     CDS             21686..21940
                     /gene="purS"
                     /locus_tag="ONT65_000271"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR2327929.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            21942..22613
                     /gene="purQ"
                     /locus_tag="ONT65_000272"
     CDS             21942..22613
                     /gene="purQ"
                     /locus_tag="ONT65_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR2327930.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            22614..24836
                     /gene="purL"
                     /locus_tag="ONT65_000273"
     CDS             22614..24836
                     /gene="purL"
                     /locus_tag="ONT65_000273"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR2327931.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            24821..26260
                     /locus_tag="ONT65_000274"
     CDS             24821..26260
                     /locus_tag="ONT65_000274"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR2327932.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            26283..27335
                     /locus_tag="ONT65_000275"
     CDS             26283..27335
                     /locus_tag="ONT65_000275"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR2327933.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            27332..27910
                     /gene="purN"
                     /locus_tag="ONT65_000276"
     CDS             27332..27910
                     /gene="purN"
                     /locus_tag="ONT65_000276"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR2327934.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            27907..29442
                     /gene="purH"
                     /locus_tag="ONT65_000277"
     CDS             27907..29442
                     /gene="purH"
                     /locus_tag="ONT65_000277"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR2327935.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            29458..30732
                     /gene="purD"
                     /locus_tag="ONT65_000278"
     CDS             29458..30732
                     /gene="purD"
                     /locus_tag="ONT65_000278"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR2327936.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            30854..31768
                     /locus_tag="ONT65_000279"
     CDS             30854..31768
                     /locus_tag="ONT65_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2327937.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(31901..33466)
                     /locus_tag="ONT65_000280"
     CDS             complement(31901..33466)
                     /locus_tag="ONT65_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2327938.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(33459..34910)
                     /locus_tag="ONT65_000281"
     CDS             complement(33459..34910)
                     /locus_tag="ONT65_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2327939.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(34924..35940)
                     /locus_tag="ONT65_000282"
     CDS             complement(34924..35940)
                     /locus_tag="ONT65_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2327940.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            36160..37248
                     /locus_tag="ONT65_000283"
     CDS             36160..37248
                     /locus_tag="ONT65_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR2327941.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            37302..38828
                     /locus_tag="ONT65_000284"
     CDS             37302..38828
                     /locus_tag="ONT65_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR2327942.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            38830..40035
                     /locus_tag="ONT65_000285"
     CDS             38830..40035
                     /locus_tag="ONT65_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2327943.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            40292..41269
                     /gene="guaC"
                     /locus_tag="ONT65_000286"
     CDS             40292..41269
                     /gene="guaC"
                     /locus_tag="ONT65_000286"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR2327944.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            41533..42957
                     /locus_tag="ONT65_000287"
     CDS             41533..42957
                     /locus_tag="ONT65_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2327945.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT65_000288"
     CDS             43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT65_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR2327946.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            44530..45507
                     /locus_tag="ONT65_000289"
     CDS             44530..45507
                     /locus_tag="ONT65_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR2327947.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            45532..47175
                     /locus_tag="ONT65_000290"
     CDS             45532..47175
                     /locus_tag="ONT65_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR2327948.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT65_000291"
     CDS             47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT65_000291"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR2327949.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            48751..49026
                     /locus_tag="ONT65_000292"
     CDS             48751..49026
                     /locus_tag="ONT65_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR2327950.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            49032..49799
                     /locus_tag="ONT65_000293"
     CDS             49032..49799
                     /locus_tag="ONT65_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR2327951.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            49967..51796
                     /gene="typA"
                     /locus_tag="ONT65_000294"
     CDS             49967..51796
                     /gene="typA"
                     /locus_tag="ONT65_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR2327952.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            52057..53829
                     /gene="recQ"
                     /locus_tag="ONT65_000295"
     CDS             52057..53829
                     /gene="recQ"
                     /locus_tag="ONT65_000295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR2327953.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            53943..54245
                     /locus_tag="ONT65_000296"
     CDS             53943..54245
                     /locus_tag="ONT65_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR2327954.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            54248..55411
                     /locus_tag="ONT65_000297"
     CDS             54248..55411
                     /locus_tag="ONT65_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2327955.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            55437..58865
                     /locus_tag="ONT65_000298"
     CDS             55437..58865
                     /locus_tag="ONT65_000298"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR2327956.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            58886..60010
                     /locus_tag="ONT65_000299"
     CDS             58886..60010
                     /locus_tag="ONT65_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327957.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            60000..60437
                     /locus_tag="ONT65_000300"
     CDS             60000..60437
                     /locus_tag="ONT65_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2327958.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            60421..60759
                     /locus_tag="ONT65_000301"
     CDS             60421..60759
                     /locus_tag="ONT65_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR2327959.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT65_000302"
     CDS             60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT65_000302"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR2327960.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            61435..61926
                     /gene="coaD"
                     /locus_tag="ONT65_000303"
     CDS             61435..61926
                     /gene="coaD"
                     /locus_tag="ONT65_000303"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR2327961.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            61916..62959
                     /locus_tag="ONT65_000304"
     CDS             61916..62959
                     /locus_tag="ONT65_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR2327962.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            63118..63846
                     /locus_tag="ONT65_000305"
     CDS             63118..63846
                     /locus_tag="ONT65_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR2327963.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            63962..64207
                     /locus_tag="ONT65_000306"
     CDS             63962..64207
                     /locus_tag="ONT65_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2327964.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            64204..64422
                     /locus_tag="ONT65_000307"
     CDS             64204..64422
                     /locus_tag="ONT65_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR2327965.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            64558..65097
                     /locus_tag="ONT65_000308"
     CDS             64558..65097
                     /locus_tag="ONT65_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR2327966.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            65090..67363
                     /locus_tag="ONT65_000309"
     CDS             65090..67363
                     /locus_tag="ONT65_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR2327967.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            67401..68447
                     /gene="holA"
                     /locus_tag="ONT65_000310"
     CDS             67401..68447
                     /gene="holA"
                     /locus_tag="ONT65_000310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR2327968.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(68536..68790)
                     /locus_tag="ONT65_000311"
     CDS             complement(68536..68790)
                     /locus_tag="ONT65_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR2327969.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(69190..69459)
                     /locus_tag="ONT65_000312"
     CDS             complement(69190..69459)
                     /locus_tag="ONT65_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327970.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(69929..70384)
                     /locus_tag="ONT65_000313"
     CDS             complement(69929..70384)
                     /locus_tag="ONT65_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR2327971.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(70522..72450)
                     /locus_tag="ONT65_000314"
     CDS             complement(70522..72450)
                     /locus_tag="ONT65_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2327972.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        23651 a        12522 c        15835 g        20621 t
ORIGIN      
        1 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
       61 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
      121 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
      181 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
      241 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
      301 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
      361 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
      421 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
      481 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
      541 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
      601 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
      661 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
      721 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
      781 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
      841 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
      901 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
      961 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
     1021 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
     1081 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
     1141 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
     1201 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
     1261 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
     1321 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
     1381 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
     1441 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
     1501 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
     1561 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
     1621 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
     1681 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
     1741 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa caaggcgtac
     1801 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
     1861 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
     1921 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
     1981 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
     2041 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
     2101 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
     2161 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
     2221 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
     2281 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
     2341 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgccaaa
     2401 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
     2461 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
     2521 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
     2581 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
     2641 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
     2701 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
     2761 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
     2821 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
     2881 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
     2941 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
     3001 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
     3061 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
     3121 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
     3181 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
     3241 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
     3301 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
     3361 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
     3421 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
     3481 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
     3541 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
     3601 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
     3661 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
     3721 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
     3781 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
     3841 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
     3901 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
     3961 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
     4021 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
     4081 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
     4141 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
     4201 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
     4261 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
     4321 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
     4381 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
     4441 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
     4501 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
     4561 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
     4621 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
     4681 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
     4741 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
     4801 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
     4861 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
     4921 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
     4981 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
     5041 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
     5101 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
     5161 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
     5221 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
     5281 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
     5341 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
     5401 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
     5461 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
     5521 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
     5581 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
     5641 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
     5701 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
     5761 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
     5821 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
     5881 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
     5941 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
     6001 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
     6061 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
     6121 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
     6181 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
     6241 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
     6301 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
     6361 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
     6421 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
     6481 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
     6541 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
     6601 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
     6661 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
     6721 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
     6781 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
     6841 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
     6901 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
     6961 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
     7021 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
     7081 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
     7141 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
     7201 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
     7261 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
     7321 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
     7381 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
     7441 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
     7501 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
     7561 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
     7621 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
     7681 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
     7741 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
     7801 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
     7861 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
     7921 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
     7981 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
     8041 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
     8101 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
     8161 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
     8221 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
     8281 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
     8341 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
     8401 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
     8461 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
     8521 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
     8581 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
     8641 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
     8701 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
     8761 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
     8821 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
     8881 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
     8941 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
     9001 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
     9061 caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta
     9121 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
     9181 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
     9241 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
     9301 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
     9361 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
     9421 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
     9481 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
     9541 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
     9601 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
     9661 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
     9721 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
     9781 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
     9841 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
     9901 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
     9961 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    10021 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    10081 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    10141 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    10201 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    10261 tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc
    10321 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    10381 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    10441 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    10501 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    10561 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    10621 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    10681 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    10741 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    10801 agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg gatcaagaca
    10861 atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg ataacagtga
    10921 taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga aatccagtct
    10981 catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt ttgaactcca
    11041 tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc tttggtctat
    11101 tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt tctttgtatt
    11161 atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg gagttgtttt
    11221 ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg ctttcctggt
    11281 ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg caattcggtt
    11341 cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat tatgaagcgg
    11401 cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta cctcttcttt
    11461 cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg acttttacgc
    11521 aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat gctctttatg
    11581 tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt tcgtggatca
    11641 tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg
    11701 ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt tcaatacggt
    11761 tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat cgcaggttct
    11821 tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga gacttttcaa
    11881 tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt tactttcttt
    11941 aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag ctcgacttgt
    12001 attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg
    12061 ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat tatttacaat
    12121 cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt tggacaagca
    12181 tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt agatgaatca
    12241 gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt tcctttgctg
    12301 aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga cgacttcatg
    12361 ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc catcaaatta
    12421 tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg
    12481 ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca ggggatcagt
    12541 acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt ctttacaagc
    12601 tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct cttttcgctt
    12661 tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt gtctatctga
    12721 tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg gcagaaaaaa
    12781 aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg ttgtttcagc
    12841 tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt ggagcttggt
    12901 atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt tttagtacgc
    12961 taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc aaaaggaata
    13021 tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt ttgtatggac
    13081 tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca gggctgccat
    13141 ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga aaaaatatgt
    13201 acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct ctacgtatca
    13261 tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg attatgcaaa
    13321 tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc catcacaatt
    13381 tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag tagatcgaac
    13441 gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc
    13501 tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac agacgggaag
    13561 aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga taaaggaact
    13621 gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac
    13681 gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata gcggcgggct
    13741 tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga acagccgtcc
    13801 ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg cggagaaacg
    13861 cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt
    13921 tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag cagaaaaaga
    13981 cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg aagatacact
    14041 aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa acgcgtatat
    14101 gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt
    14161 gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag cagccgttct
    14221 tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga
    14281 agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg gtgaagaaaa
    14341 agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt cgattttgac
    14401 aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg acgagcttgt
    14461 cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta taaaatcttg
    14521 ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg gctatgacat
    14581 gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga aaataaaaat
    14641 aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat tttacaaaaa
    14701 aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc aatatttgaa
    14761 acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa
    14821 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
    14881 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
    14941 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
    15001 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
    15061 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
    15121 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
    15181 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
    15241 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
    15301 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
    15361 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
    15421 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
    15481 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
    15541 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
    15601 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
    15661 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
    15721 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
    15781 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
    15841 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
    15901 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
    15961 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
    16021 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
    16081 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
    16141 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
    16201 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
    16261 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
    16321 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
    16381 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
    16441 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
    16501 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
    16561 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
    16621 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
    16681 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
    16741 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
    16801 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
    16861 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
    16921 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
    16981 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
    17041 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    17101 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    17161 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    17221 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    17281 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    17341 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    17401 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    17461 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    17521 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    17581 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    17641 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    17701 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    17761 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    17821 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
    17881 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    17941 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    18001 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    18061 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    18121 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    18181 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    18241 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    18301 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    18361 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    18421 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    18481 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    18541 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    18601 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    18661 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    18721 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    18781 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    18841 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    18901 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    18961 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    19021 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    19081 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    19141 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    19201 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    19261 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    19321 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    19381 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    19441 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
    19501 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    19561 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    19621 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    19681 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    19741 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    19801 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    19861 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    19921 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    19981 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    20041 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    20101 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    20161 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    20221 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    20281 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    20341 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    20401 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    20461 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    20521 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    20581 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    20641 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    20701 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
    20761 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    20821 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    20881 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    20941 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    21001 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    21061 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    21121 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    21181 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    21241 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    21301 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    21361 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
    21421 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    21481 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    21541 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    21601 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    21661 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    21721 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    21781 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    21841 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    21901 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    21961 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    22021 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    22081 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    22141 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    22201 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    22261 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    22321 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    22381 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    22441 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    22501 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    22561 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    22621 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    22681 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    22741 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    22801 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    22861 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    22921 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    22981 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    23041 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    23101 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    23161 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    23221 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    23281 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    23341 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    23401 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    23461 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    23521 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    23581 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    23641 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    23701 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    23761 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    23821 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    23881 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    23941 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    24001 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    24061 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    24121 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    24181 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    24241 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    24301 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    24361 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    24421 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    24481 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    24541 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    24601 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    24661 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    24721 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    24781 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    24841 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    24901 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    24961 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    25021 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    25081 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    25141 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    25201 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    25261 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    25321 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    25381 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    25441 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    25501 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    25561 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    25621 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    25681 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    25741 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    25801 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    25861 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    25921 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    25981 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    26041 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    26101 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    26161 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    26221 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    26281 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    26341 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    26401 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    26461 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    26521 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    26581 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    26641 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    26701 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    26761 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    26821 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    26881 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    26941 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    27001 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    27061 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    27121 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    27181 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    27241 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    27301 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    27361 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    27421 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    27481 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    27541 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    27601 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    27661 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    27721 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    27781 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    27841 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    27901 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    27961 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    28021 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    28081 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    28141 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    28201 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    28261 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    28321 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    28381 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    28441 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    28501 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    28561 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    28621 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    28681 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    28741 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    28801 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    28861 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    28921 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    28981 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    29041 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    29101 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    29161 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    29221 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    29281 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    29341 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    29401 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    29461 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    29521 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    29581 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    29641 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    29701 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    29761 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    29821 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    29881 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    29941 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    30001 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    30061 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    30121 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    30181 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    30241 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    30301 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    30361 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    30421 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    30481 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    30541 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    30601 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    30661 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    30721 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    30781 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    30841 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    30901 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    30961 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    31021 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    31081 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    31141 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    31201 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    31261 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    31321 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    31381 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    31441 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    31501 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    31561 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    31621 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    31681 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    31741 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    31801 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    31861 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    31921 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    31981 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    32041 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    32101 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    32161 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    32221 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    32281 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    32341 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    32401 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    32461 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    32521 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    32581 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    32641 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    32701 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    32761 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    32821 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    32881 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    32941 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    33001 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    33061 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    33121 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    33181 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    33241 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    33301 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    33361 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    33421 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    33481 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    33541 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    33601 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    33661 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    33721 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    33781 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    33841 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    33901 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    33961 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    34021 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    34081 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    34141 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    34201 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    34261 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    34321 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    34381 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    34441 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    34501 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    34561 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    34621 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    34681 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    34741 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    34801 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    34861 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    34921 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    34981 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    35041 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    35101 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    35161 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    35221 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    35281 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    35341 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    35401 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    35461 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    35521 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    35581 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    35641 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    35701 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    35761 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    35821 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    35881 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    35941 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    36001 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    36061 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    36121 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    36181 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    36241 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    36301 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    36361 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    36421 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    36481 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    36541 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    36601 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    36661 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    36721 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    36781 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    36841 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    36901 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    36961 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    37021 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    37081 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    37141 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    37201 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    37261 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    37321 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    37381 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    37441 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    37501 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    37561 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    37621 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    37681 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    37741 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    37801 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    37861 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    37921 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    37981 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    38041 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    38101 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    38161 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    38221 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    38281 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    38341 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    38401 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    38461 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    38521 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    38581 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    38641 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    38701 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    38761 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    38821 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    38881 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    38941 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    39001 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    39061 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    39121 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    39181 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    39241 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    39301 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    39361 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    39421 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    39481 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    39541 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    39601 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    39661 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    39721 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    39781 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    39841 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    39901 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    39961 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    40021 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    40081 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    40141 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    40201 tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat gttcgtgttt
    40261 tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg aagatattca
    40321 attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt
    40381 aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga ttattgatga
    40441 aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc ggtttgatga
    40501 agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag
    40561 tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag aatcgctaaa
    40621 tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta ttgatatgat
    40681 tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc
    40741 ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc
    40801 aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct ggcaactggc
    40861 agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg gtgggatcag
    40921 aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca tgattggttc
    40981 attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta
    41041 taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg
    41101 aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg aaatgcaaca
    41161 agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca ttcgaaaagt
    41221 tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa aaataaataa
    41281 aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag ccaaacaaaa
    41341 atagttcttt atatgttttg tttggctgga attattatcg cagatacaag cttttgaaca
    41401 tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta tgttttgcaa
    41461 aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga
    41521 aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt
    41581 atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag tcaacttgaa
    41641 tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt ccatgcattg
    41701 gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg gtttgttaat
    41761 cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat gatctctcct
    41821 cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca gatccaaaaa
    41881 acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc gaattttgag
    41941 tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga tcttttaggc
    42001 tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga attggcattt
    42061 tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt gattttacaa
    42121 acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt ttaccgatta
    42181 cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga agaagggatc
    42241 gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt gcttatcaac
    42301 ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga aaagctagta
    42361 gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac ggatctttca
    42421 tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa tgtgttatac
    42481 gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt gataggacct
    42541 gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc gatgaatatc
    42601 cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa aatgcaagat
    42661 gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga ttcacaatcg
    42721 tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg ggatgggaaa
    42781 aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat tgcaccaagt
    42841 ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct ggatgaaaac
    42901 caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac agaataattt
    42961 cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag ctgtttcttt
    43021 tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt tgtacgtccg
    43081 ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc aacgttttta
    43141 aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa
    43201 aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat tggcataatt
    43261 tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc atgataattt
    43321 attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc gcagaatgaa
    43381 gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat
    43441 cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc aagcacttga
    43501 tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg atgaactcgt
    43561 tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa cagcgctgaa
    43621 ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa gtcagctggc
    43681 tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg atgtacctca
    43741 actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag gacatgctgc
    43801 aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca tcggtgcaca
    43861 atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt
    43921 cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag caatcaattt
    43981 cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt atgcgatctc
    44041 tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg
    44101 gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg tatctaaaat
    44161 ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac tgacttatcg
    44221 ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa aagacacaga
    44281 tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga cggaaaaagg
    44341 actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag aaatcaaagc
    44401 agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat
    44461 gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag cgaaggagtc
    44521 gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt agcacttgaa
    44581 ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt
    44641 ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt cgatactcct
    44701 ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg atttcgtcca
    44761 gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt agtagctcaa
    44821 atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac aattcgttcg
    44881 ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga aggtttgatc
    44941 gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc aaaaggacta
    45001 ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat gaaactatat
    45061 cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga taaagcagca
    45121 gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt gcgtgaagca
    45181 atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt
    45241 acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac tggtcgcgta
    45301 gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc
    45361 tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc acctgataca
    45421 gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac
    45481 aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt catggcttat
    45541 caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    45601 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    45661 tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat tgttccagaa
    45721 ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg acacgaagac
    45781 aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc ggctgctgaa
    45841 cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca atttaagtta
    45901 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    45961 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    46021 attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg cactgtagca
    46081 aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc accgtcaact
    46141 agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc agcaagtgtc
    46201 gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg tcaatttgcc
    46261 cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg cagagtaaca
    46321 aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc gaaatcagaa
    46381 gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa accagctgca
    46441 ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc aatgacgcca
    46501 acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc acacgtgaca
    46561 ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt taaagaagtt
    46621 gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc tttaacagcg
    46681 actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca agaaatcgtt
    46741 tataaacatt actacaatat cggtattgca actgatacgg atcacggact atatgtacca
    46801 aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat caatgagaaa
    46861 gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg aacaattacg
    46921 atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa ctatccagaa
    46981 gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc tgaaggcgaa
    47041 atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat cgtagatgga
    47101 gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc agagctatta
    47161 atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat tagatacagt
    47221 tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg aaatgggaca
    47281 aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg tcggctgtat
    47341 tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag attctgaaat
    47401 gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag aatggaaaga
    47461 aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa aacataaggt
    47521 tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg tcattcatcc
    47581 agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt cccgtccaat
    47641 cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg gattagcatt
    47701 gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg ccgaattggg
    47761 cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac cacaaatttt
    47821 accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga aaaaaggcgt
    47881 aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca gcgtgacagt
    47941 gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga tggtcactgt
    48001 aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg aagtaggaga
    48061 acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca tctatgccat
    48121 cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag caaaaatcgc
    48181 tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc cggcagtagc
    48241 cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa aagatgctgg
    48301 tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat tatcgcttgg
    48361 taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc ttattggcgc
    48421 gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag caatcgaatc
    48481 aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc ttggcgaaat
    48541 cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat ttgaataata
    48601 caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat caataatatt
    48661 attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct atgctacact
    48721 gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc acttgattta
    48781 gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct ggaacgtgcg
    48841 aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt taaaactgtt
    48901 gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc ttccggttat
    48961 tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac
    49021 gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa gaagctggtg
    49081 agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat cgaacggatc
    49141 tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc cattcctttg
    49201 atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg gatctcgatg
    49261 gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa
    49321 atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt ttcatttatg
    49381 acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg
    49441 agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt gtgaatagtt
    49501 atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc atggggacac
    49561 gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac cacattgctt
    49621 atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg gaagagtttg
    49681 gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg gaatactatt
    49741 tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa
    49801 aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa
    49861 aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag cagtcgagta
    49921 aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga attatagaaa
    49981 cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa cattagttga
    50041 tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag aacgtgcgat
    50101 ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa acacagctgt
    50161 agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg acttcggtgg
    50221 cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag atgcgtatga
    50281 aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac atatcacacc
    50341 aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga
    50401 agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact ttcctgtgat
    50461 ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag atcaagaacc
    50521 aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg tcgataacag
    50581 cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg taggtcgtat
    50641 cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag cattaattaa
    50701 attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct ttggactaaa
    50761 acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag gtatggaaga
    50821 tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac ctatccttca
    50881 cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat ttgccggtcg
    50941 agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac
    51001 agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag tttctggtcg
    51061 tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat acgaattgca
    51121 agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga
    51181 acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt ccttgagctt
    51241 acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc gtttgacatt
    51301 cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg
    51361 ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg gtcaaattgg
    51421 cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga cttattctat
    51481 catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag tatatgaagg
    51541 aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca cgaaagcaaa
    51601 acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa
    51661 acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt gcgaagtaac
    51721 accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc
    51781 taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt ataaaaaata
    51841 gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt caatggacat
    51901 aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta tttctagaga
    51961 gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa
    52021 aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt aaaacaatac
    52081 tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt cattgatcag
    52141 gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta ccagcttcca
    52201 gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt gatgaaggat
    52261 caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag ctcgttagat
    52321 ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg tttactttat
    52381 atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg atggaacgtc
    52441 cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga tttccgccca
    52501 agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt aattgttgct
    52561 ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt aaaaattcca
    52621 gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca agtaattaaa
    52681 gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt
    52741 atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct taaaaaattc
    52801 gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa
    52861 gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt tggtatgggt
    52921 ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc tctagaagca
    52981 tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc catcctgctt
    53041 ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca aagagaagaa
    53101 gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt gtatattgag
    53161 tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc gtgcaaccgt
    53221 tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc acagatggtt
    53281 ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat gaaagtctta
    53341 gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc tacctatgga
    53401 ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt gatatcgaat
    53461 ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga aagagggata
    53521 caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt ccagcagcta
    53581 tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac ggatctagca
    53641 tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa agaaatgagc
    53701 cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg ccaaagcaag
    53761 cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc taacgttctg
    53821 gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa atctgttttt
    53881 tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg aggtaagcac
    53941 agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa gcagaacgga
    54001 tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc aaagccttcg
    54061 aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt gctgtccgta
    54121 taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa cgagaattga
    54181 atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt ggcaaggagg
    54241 aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca tcttgattcc
    54301 ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt cgtatctatt
    54361 attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt tttgggtgct
    54421 tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga aaaaccagcg
    54481 tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg tgttttttgg
    54541 taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga tccaacctgc
    54601 cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta aaagacaaaa
    54661 cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg ttatcggact
    54721 tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct ttttgattat
    54781 tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg taggagtagg
    54841 aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg gaaagatctt
    54901 acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc ctttctcaga
    54961 cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca acggaggact
    55021 atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag aagcacaaac
    55081 cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga ttttgatatt
    55141 agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc
    55201 gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa
    55261 tcttggagga attactggcg ttattccttt gacagggatc acgttcccat ttttaagtca
    55321 aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc
    55381 ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt ggttcgatga
    55441 aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag
    55501 aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc gtacaccgtt
    55561 tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat gcgtatctag
    55621 atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt catccgggtt
    55681 acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt
    55741 ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa gcaaaagctg
    55801 cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc gcttctatag
    55861 acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag gcagctctag
    55921 gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg
    55981 aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat
    56041 acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat ggaaacgtca
    56101 tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta gtagaagtag
    56161 cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat
    56221 tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt gaaggagatg
    56281 atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc acagaaatga
    56341 ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg gatctgcata
    56401 aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc attcaatgcc
    56461 gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa atcgatacgt
    56521 atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg
    56581 tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt gctacctttg
    56641 aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa
    56701 caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca ggaaatgcaa
    56761 aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta cgagaccgcg
    56821 gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa
    56881 gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc acaagaactg
    56941 attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg gtcgaatgga
    57001 tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc catcaaagtc
    57061 tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg actggagaag
    57121 gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat gtggcttacc
    57181 gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta atgcctaata
    57241 cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat tatcctgaca
    57301 atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta tttcgaatct
    57361 ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta cgggatactg
    57421 gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca agtcgggcaa
    57481 aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg ggtgcgcata
    57541 tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat cgtttgatta
    57601 gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat accagcggga
    57661 atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa
    57721 tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat tatgcactgg
    57781 tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta aaccattatt
    57841 gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag
    57901 aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa caggcaaaag
    57961 ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact gtcaacttga
    58021 tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat atggcactat
    58081 ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa acactaagtt
    58141 tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt ggcggatttc
    58201 ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa cgtccaggtc
    58261 ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa aaaattggct
    58321 atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc tttttggact
    58381 atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca acatttttcc
    58441 aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa acgcttatca
    58501 ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt
    58561 tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt acggtccaaa
    58621 cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg tctggttctg
    58681 ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg ttgatgatta
    58741 ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa gtcgcacatg
    58801 tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa gtgactgaga
    58861 aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc tgattctgct
    58921 attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa
    58981 ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc aggagcttaa
    59041 agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa aggattttgg
    59101 ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt atgaaaataa
    59161 agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa ctgttaaagt
    59221 actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga tgcaagatct
    59281 tacgaaaatc actactatct atactaattt cacttttgag taccatgata cagaagttgg
    59341 aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg ataacgacac
    59401 ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg tgtatttaga
    59461 taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc ttccacatta
    59521 tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac gaaaaagtac
    59581 gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc aaacacttgc
    59641 ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg aggaaatatt
    59701 gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa
    59761 attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat tagaaaatac
    59821 tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat attggtacag
    59881 cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag gcattgcttt
    59941 ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta gtgatagcag
    60001 tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc gctgaaatga
    60061 taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg gaagaatctg
    60121 caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt gaaaatgtag
    60181 cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa
    60241 aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa acacagctgc
    60301 aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat gaaatcaaag
    60361 tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga ggtaattgtc
    60421 atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat cgtatgggtc
    60481 tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta tgtctcaaga
    60541 agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac agaagaacga
    60601 atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga tatcgaaatg
    60661 aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa
    60721 gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat gagcagtttt
    60781 taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt atttttttgg
    60841 tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat caggaaatta
    60901 tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta cagataaagt
    60961 aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag tattggatct
    61021 gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca gtcatgcggt
    61081 atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa
    61141 agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg aacggctagc
    61201 agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa aacaaaaaat
    61261 aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg cagttatcgt
    61321 atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc atcaaataag
    61381 aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa atcaatgaaa
    61441 cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga tacggtggaa
    61501 cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac ttcaaagaaa
    61561 agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc tcatcttcct
    61621 aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa agagattggt
    61681 gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga acgagagatt
    61741 gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt agcaaaacca
    61801 gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg cggagatgtg
    61861 agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa
    61921 agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga tcattggatt
    61981 tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg atctgaaaga
    62041 actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc ttacctctgt
    62101 tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc cttttgaaga
    62161 attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata atcaaatgca
    62221 acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga
    62281 caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa
    62341 tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga aaagctttca
    62401 aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa caatcgaaat
    62461 cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg agttaccaac
    62521 agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg aatcagatac
    62581 agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga tgttcacttt
    62641 agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac
    62701 tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc
    62761 aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga tttcagacga
    62821 tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag ctgccgctaa
    62881 aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta
    62941 tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg agttctgtca
    63001 caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag aaaaaattga
    63061 cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca attttaaatg
    63121 aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat gatagaaaaa
    63181 atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat gatagttctt
    63241 atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag agaaacagaa
    63301 gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga tccatcagct
    63361 gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga
    63421 agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta tgatgtacta
    63481 aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt ttctgcgaag
    63541 atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc agaacatctt
    63601 gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc tcccaaaata
    63661 aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg atcagtcaaa
    63721 gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt ggaagacctg
    63781 caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt
    63841 aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat ggatactaag
    63901 aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga taggaaatag
    63961 cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc tccaaaataa
    64021 aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc aagctctacg
    64081 agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta ttctcattgg
    64141 gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa aaatctgcag
    64201 tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt tatcacgcca
    64261 ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa agaatatatc
    64321 tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt tatacggaat
    64381 ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac ggacttcatt
    64441 tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt taaagaatat
    64501 tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat ataaaacatg
    64561 ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga taagtcacat
    64621 aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt atctttacgt
    64681 agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag gatcattgct
    64741 ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg gtgttacgtc
    64801 gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct acagtgtgca
    64861 aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc atgtttggcc
    64921 tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca tgactatcga
    64981 aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca ccaagttgct
    65041 ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt
    65101 tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga tgattatctg
    65161 tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa tttttttatg
    65221 gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta gcataacggt
    65281 atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa catctgcgca
    65341 aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa tggaaggaag
    65401 gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag aagcagaatt
    65461 gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag gcgagttcat
    65521 ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact tcaggaacca
    65581 tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc aaaatcactg
    65641 gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat ctgaatttcc
    65701 gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg caggattcag
    65761 agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc tatcgggtct
    65821 acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg gatggccact
    65881 acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta ttgcaggaag
    65941 ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag tcaaattgct
    66001 gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc ttttactatt
    66061 tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga gttttttgtt
    66121 tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg tcctgtttac
    66181 tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt taggtagtct
    66241 ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg tgttatttct
    66301 cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag agggattaat
    66361 cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag gacgtttgcc
    66421 ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata ggttgaaaga
    66481 acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt tgactttgcc
    66541 atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt
    66601 tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc agttacactt
    66661 tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa atatcgttcc
    66721 atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac acgacgatac
    66781 ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa aaatttatat
    66841 cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa
    66901 tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt atgatcttta
    66961 tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg gtctatggat
    67021 ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa tggaaaaaca
    67081 attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc atcacggtag
    67141 ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg ggattatatc
    67201 ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga cactagaaga
    67261 aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc gctggcatcc
    67321 agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct catctgctca
    67381 tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag ttacaaaaaa
    67441 tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag tttctccaag
    67501 atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt
    67561 ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa gcagagacgt
    67621 tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt ttaacaggtg
    67681 aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat ttgaaagaac
    67741 cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat gaacggaaaa
    67801 aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa
    67861 aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa atggaacgaa
    67921 gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg atgagagagt
    67981 tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga gaagtagaag
    68041 aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat attttaaaag
    68101 ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg gaagaaacaa
    68161 tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca aaatttcttg
    68221 taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg
    68281 taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg
    68341 atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt
    68401 ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa gaaagacgag
    68461 ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg
    68521 ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt cgcggttagc
    68581 tttgtttttg tggattagac cttttgattc agccatatca atagctttaa cagcttcttt
    68641 atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt ttttgattgc
    68701 agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc ttgtacgaac
    68761 acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact taagttctta
    68821 cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt gctttgcaat
    68881 atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt tcagtaaaaa
    68941 cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa
    69001 ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt ttggtactaa
    69061 gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga tacgattccc
    69121 tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt atcctttgat
    69181 cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat tttttatcaa
    69241 catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt ccagcacata
    69301 tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa gcaggccatt
    69361 gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct gttcttgtaa
    69421 aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc tttcttttca
    69481 aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag tggatgcgat
    69541 cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag agccaaaaag
    69601 aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt attacaaaac
    69661 attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt aataggtcaa
    69721 tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat aagctaagcc
    69781 cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat actttaattt
    69841 ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg ggggtgttaa
    69901 gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact tgtaagatac
    69961 agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag aagacaacac
    70021 ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca atcaatccta
    70081 cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc cagccaatag
    70141 gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg atagggatta
    70201 gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga agccaaggga
    70261 aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc gctatcatca
    70321 gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa agccaacttt
    70381 ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta gaaagttaca
    70441 aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata aaataaacaa
    70501 aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt atagtggaca
    70561 agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc
    70621 attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag gtagatttaa
    70681 aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa aaataatatt
    70741 ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa atttatttaa
    70801 gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc cttgccccat
    70861 agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat caccaaccat
    70921 cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt cttccggagt
    70981 acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg ctgtgccaga
    71041 atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg aaattacttg
    71101 ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact gctgatcttt
    71161 agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc
    71221 tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc attgcgcacc
    71281 taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt attggatttc
    71341 ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt
    71401 ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc
    71461 agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa
    71521 ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt ttgcagaatt
    71581 tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag gagaagccac
    71641 aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg
    71701 ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat tattttcgat
    71761 tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta ctaattggat
    71821 aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga tgggatttcc
    71881 ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag gcacagattc
    71941 accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg
    72001 aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag aaacatctac
    72061 ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa gattcatcaa
    72121 agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac cgcttaaaca
    72181 aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg caccaatagc
    72241 agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt ctttcaagcc
    72301 ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa gtggggtgat
    72361 ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc agccgattgt
    72421 tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc aaaacaagga
    72481 tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat aatggttatc
    72541 atttggtgat taagcttgat gttgttttta ttatttataa tgattattat taaagaaaat
    72601 aaaaaaccag aacatttctc aaataagag
//
LOCUS       DAJSBC010000005        71080 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSBC010000005 DAJSBC010000000
VERSION     DAJSBC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.005"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            161..391
                     /locus_tag="ONT65_000315"
     CDS             161..391
                     /locus_tag="ONT65_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR2327973.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="ONT65_000316"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="ONT65_000316"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2327974.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="ONT65_000317"
     CDS             1802..1978
                     /locus_tag="ONT65_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2327975.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="ONT65_000318"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="ONT65_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR2327976.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="ONT65_000319"
     CDS             2593..3060
                     /locus_tag="ONT65_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2327977.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="ONT65_000320"
     CDS             3145..4029
                     /locus_tag="ONT65_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2327978.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="ONT65_000321"
     CDS             4143..4379
                     /locus_tag="ONT65_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR2327979.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="ONT65_000322"
     tRNA            4467..4547
                     /locus_tag="ONT65_000322"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="ONT65_000323"
     tRNA            4554..4625
                     /locus_tag="ONT65_000323"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="ONT65_000324"
     CDS             4750..4920
                     /locus_tag="ONT65_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327980.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="ONT65_000325"
     CDS             5140..5847
                     /locus_tag="ONT65_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2327981.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="ONT65_000326"
     CDS             complement(5907..6551)
                     /locus_tag="ONT65_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR2327982.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="ONT65_000327"
     CDS             6678..6965
                     /locus_tag="ONT65_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2327983.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="ONT65_000328"
     CDS             7073..7393
                     /locus_tag="ONT65_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2327984.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="ONT65_000329"
     CDS             7474..8787
                     /locus_tag="ONT65_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2327985.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="ONT65_000330"
     CDS             8784..9503
                     /locus_tag="ONT65_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR2327986.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="ONT65_000331"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="ONT65_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR2327987.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="ONT65_000332"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="ONT65_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR2327988.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="ONT65_000333"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="ONT65_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR2327989.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT65_000334"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT65_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR2327990.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="ONT65_000335"
     CDS             14940..15833
                     /locus_tag="ONT65_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2327991.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="ONT65_000336"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="ONT65_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2327992.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="ONT65_000337"
     CDS             17770..18627
                     /locus_tag="ONT65_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR2327993.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="ONT65_000338"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="ONT65_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR2327994.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="ONT65_000339"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="ONT65_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR2327995.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="ONT65_000340"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="ONT65_000340"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2327996.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="ONT65_000341"
     CDS             21383..22141
                     /locus_tag="ONT65_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2327997.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="ONT65_000342"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="ONT65_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR2327998.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="ONT65_000343"
     CDS             23069..24100
                     /locus_tag="ONT65_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2327999.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="ONT65_000344"
     CDS             24097..24864
                     /locus_tag="ONT65_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2328000.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="ONT65_000345"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="ONT65_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR2328001.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="ONT65_000346"
     CDS             26829..27149
                     /locus_tag="ONT65_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR2328002.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="ONT65_000347"
     CDS             27149..27499
                     /locus_tag="ONT65_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR2328003.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="ONT65_000348"
     CDS             27666..28604
                     /locus_tag="ONT65_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR2328004.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="ONT65_000349"
     CDS             28619..29449
                     /locus_tag="ONT65_000349"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR2328005.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="ONT65_000350"
     CDS             29493..30518
                     /locus_tag="ONT65_000350"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2328006.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="ONT65_000351"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="ONT65_000351"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2328007.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="ONT65_000352"
     CDS             31596..32036
                     /locus_tag="ONT65_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR2328008.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="ONT65_000353"
     CDS             32039..32641
                     /locus_tag="ONT65_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2328009.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="ONT65_000354"
     CDS             complement(32700..33806)
                     /locus_tag="ONT65_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2328010.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT65_000355"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT65_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR2328011.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="ONT65_000356"
     CDS             complement(35354..37699)
                     /locus_tag="ONT65_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2328012.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="ONT65_000357"
     CDS             37978..38880
                     /locus_tag="ONT65_000357"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR2328013.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="ONT65_000358"
     CDS             39093..40868
                     /locus_tag="ONT65_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR2328014.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="ONT65_000359"
     CDS             complement(40934..42520)
                     /locus_tag="ONT65_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR2328015.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="ONT65_000360"
     CDS             42771..42995
                     /locus_tag="ONT65_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR2328016.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="ONT65_000361"
     CDS             43137..44888
                     /locus_tag="ONT65_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328017.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="ONT65_000362"
     CDS             44888..46672
                     /locus_tag="ONT65_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328018.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="ONT65_000363"
     CDS             46998..47849
                     /locus_tag="ONT65_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2328019.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="ONT65_000364"
     CDS             47903..49654
                     /locus_tag="ONT65_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2328020.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="ONT65_000365"
     CDS             49658..49828
                     /locus_tag="ONT65_000365"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2328021.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="ONT65_000366"
     CDS             complement(49868..50770)
                     /locus_tag="ONT65_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2328022.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="ONT65_000367"
     CDS             51001..52404
                     /locus_tag="ONT65_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2328023.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /locus_tag="ONT65_000368"
     CDS             52404..53837
                     /locus_tag="ONT65_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2328024.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="ONT65_000369"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="ONT65_000369"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR2328025.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="ONT65_000370"
     CDS             55122..56849
                     /locus_tag="ONT65_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR2328026.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56864..57214
                     /locus_tag="ONT65_000371"
     CDS             56864..57214
                     /locus_tag="ONT65_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2328027.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="ONT65_000372"
     CDS             complement(57306..58166)
                     /locus_tag="ONT65_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR2328028.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="ONT65_000373"
     CDS             58258..58686
                     /locus_tag="ONT65_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2328029.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="ONT65_000374"
     CDS             58848..59024
                     /locus_tag="ONT65_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR2328030.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="ONT65_000375"
     CDS             59051..59497
                     /locus_tag="ONT65_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR2328031.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT65_000376"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT65_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2328032.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="ONT65_000377"
     CDS             61736..62707
                     /locus_tag="ONT65_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR2328033.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="ONT65_000378"
     CDS             62731..64923
                     /locus_tag="ONT65_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR2328034.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT65_000379"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT65_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR2328035.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65400..65795
                     /locus_tag="ONT65_000380"
     CDS             65400..65795
                     /locus_tag="ONT65_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR2328036.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /gene="era"
                     /locus_tag="ONT65_000381"
     CDS             65810..66709
                     /gene="era"
                     /locus_tag="ONT65_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR2328037.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="ONT65_000382"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="ONT65_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR2328038.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT65_000383"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT65_000383"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR2328039.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="ONT65_000384"
     CDS             68967..71042
                     /locus_tag="ONT65_000384"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR2328040.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23266 a        12442 c        15206 g        20166 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DAJSBC010000006        66048 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSBC010000006 DAJSBC010000000
VERSION     DAJSBC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.006"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(27..725)
                     /locus_tag="ONT65_000385"
     CDS             complement(27..725)
                     /locus_tag="ONT65_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR2328041.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ONT65_000386"
     CDS             complement(718..2070)
                     /locus_tag="ONT65_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR2328042.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ONT65_000387"
     CDS             complement(2293..3693)
                     /locus_tag="ONT65_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR2328043.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ONT65_000388"
     CDS             3854..4591
                     /locus_tag="ONT65_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR2328044.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ONT65_000389"
     CDS             4716..5195
                     /locus_tag="ONT65_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR2328045.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ONT65_000390"
     CDS             5192..5959
                     /locus_tag="ONT65_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328046.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ONT65_000391"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ONT65_000391"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2328047.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ONT65_000392"
     CDS             complement(6862..7464)
                     /locus_tag="ONT65_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2328048.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="ONT65_000393"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="ONT65_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="ONT65_000394"
     CDS             8145..8729
                     /locus_tag="ONT65_000394"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2328049.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ONT65_000395"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ONT65_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR2328050.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ONT65_000396"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ONT65_000396"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR2328051.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ONT65_000397"
     CDS             10630..10809
                     /locus_tag="ONT65_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328052.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ONT65_000398"
     CDS             10831..11856
                     /locus_tag="ONT65_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR2328053.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="ONT65_000399"
     CDS             12031..13275
                     /locus_tag="ONT65_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR2328054.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="ONT65_000400"
     CDS             13428..14375
                     /locus_tag="ONT65_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2328055.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="ONT65_000401"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="ONT65_000401"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR2328056.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="ONT65_000402"
     CDS             complement(15277..15519)
                     /locus_tag="ONT65_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328057.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="ONT65_000403"
     tRNA            15640..15712
                     /locus_tag="ONT65_000403"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="ONT65_000404"
     CDS             complement(15831..16469)
                     /locus_tag="ONT65_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2328058.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="ONT65_000405"
     CDS             complement(16592..17224)
                     /locus_tag="ONT65_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2328059.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="ONT65_000406"
     CDS             17575..17874
                     /locus_tag="ONT65_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2328060.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="ONT65_000407"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="ONT65_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR2328061.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="ONT65_000408"
     CDS             complement(20806..21291)
                     /locus_tag="ONT65_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR2328062.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21381..21726
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21771..22412
                     /locus_tag="ONT65_000409"
     CDS             21771..22412
                     /locus_tag="ONT65_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2328063.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="ONT65_000410"
     CDS             22431..23066
                     /locus_tag="ONT65_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2328064.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="ONT65_000411"
     CDS             23084..23899
                     /locus_tag="ONT65_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2328065.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24218..25654
                     /locus_tag="ONT65_000412"
     CDS             24218..25654
                     /locus_tag="ONT65_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2328066.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25843..28290
                     /locus_tag="ONT65_000413"
     CDS             25843..28290
                     /locus_tag="ONT65_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR2328067.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28520..29587
                     /gene="rlmN"
                     /locus_tag="ONT65_000414"
     CDS             28520..29587
                     /gene="rlmN"
                     /locus_tag="ONT65_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR2328068.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29780..30883)
                     /locus_tag="ONT65_000415"
     CDS             complement(29780..30883)
                     /locus_tag="ONT65_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2328069.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31436..31984
                     /gene="aac(6')"
                     /locus_tag="ONT65_000416"
     CDS             31436..31984
                     /gene="aac(6')"
                     /locus_tag="ONT65_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2328070.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32530..34191
                     /locus_tag="ONT65_000417"
     CDS             32530..34191
                     /locus_tag="ONT65_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2328071.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34261..34608)
                     /locus_tag="ONT65_000418"
     CDS             complement(34261..34608)
                     /locus_tag="ONT65_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR2328072.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34692..35633
                     /locus_tag="ONT65_000419"
     CDS             34692..35633
                     /locus_tag="ONT65_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328073.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35634..36677
                     /locus_tag="ONT65_000420"
     CDS             35634..36677
                     /locus_tag="ONT65_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328074.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36683..37753
                     /locus_tag="ONT65_000421"
     CDS             36683..37753
                     /locus_tag="ONT65_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328075.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37743..38696
                     /locus_tag="ONT65_000422"
     CDS             37743..38696
                     /locus_tag="ONT65_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328076.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38708..39718
                     /locus_tag="ONT65_000423"
     CDS             38708..39718
                     /locus_tag="ONT65_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2328077.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(40003..40824)
                     /locus_tag="ONT65_000424"
     CDS             complement(40003..40824)
                     /locus_tag="ONT65_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2328078.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40921..41199)
                     /locus_tag="ONT65_000425"
     CDS             complement(40921..41199)
                     /locus_tag="ONT65_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328079.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41467..43476
                     /gene="metG"
                     /locus_tag="ONT65_000426"
     CDS             41467..43476
                     /gene="metG"
                     /locus_tag="ONT65_000426"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR2328080.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(43955..44542)
                     /locus_tag="ONT65_000427"
     CDS             complement(43955..44542)
                     /locus_tag="ONT65_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328081.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            44717..46039
                     /locus_tag="ONT65_000428"
     CDS             44717..46039
                     /locus_tag="ONT65_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2328082.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            46194..46970
                     /locus_tag="ONT65_000429"
     CDS             46194..46970
                     /locus_tag="ONT65_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2328083.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            46963..47532
                     /gene="rnmV"
                     /locus_tag="ONT65_000430"
     CDS             46963..47532
                     /gene="rnmV"
                     /locus_tag="ONT65_000430"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR2328084.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            47525..48409
                     /gene="rsmA"
                     /locus_tag="ONT65_000431"
     CDS             47525..48409
                     /gene="rsmA"
                     /locus_tag="ONT65_000431"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR2328085.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            48860..49489
                     /locus_tag="ONT65_000432"
     CDS             48860..49489
                     /locus_tag="ONT65_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328086.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="ONT65_000433"
     CDS             complement(49613..50722)
                     /gene="ugpC"
                     /locus_tag="ONT65_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR2328087.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            50931..51353
                     /gene="mgsA"
                     /locus_tag="ONT65_000434"
     CDS             50931..51353
                     /gene="mgsA"
                     /locus_tag="ONT65_000434"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR2328088.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            51676..52212
                     /locus_tag="ONT65_000435"
     CDS             51676..52212
                     /locus_tag="ONT65_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2328089.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(52252..54015)
                     /locus_tag="ONT65_000436"
     CDS             complement(52252..54015)
                     /locus_tag="ONT65_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328090.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(54018..55733)
                     /locus_tag="ONT65_000437"
     CDS             complement(54018..55733)
                     /locus_tag="ONT65_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328091.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(56512..56736)
                     /locus_tag="ONT65_000438"
     CDS             complement(56512..56736)
                     /locus_tag="ONT65_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328092.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            56927..59704
                     /locus_tag="ONT65_000439"
     CDS             56927..59704
                     /locus_tag="ONT65_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2328093.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            59785..60204
                     /locus_tag="ONT65_000440"
     CDS             59785..60204
                     /locus_tag="ONT65_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR2328094.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            60216..60686
                     /locus_tag="ONT65_000441"
     CDS             60216..60686
                     /locus_tag="ONT65_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2328095.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            60703..61482
                     /locus_tag="ONT65_000442"
     CDS             60703..61482
                     /locus_tag="ONT65_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2328096.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            61469..62290
                     /locus_tag="ONT65_000443"
     CDS             61469..62290
                     /locus_tag="ONT65_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2328097.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            62307..63356
                     /locus_tag="ONT65_000444"
     CDS             62307..63356
                     /locus_tag="ONT65_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2328098.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            63369..64373
                     /locus_tag="ONT65_000445"
     CDS             63369..64373
                     /locus_tag="ONT65_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2328099.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            65006..66025
                     /locus_tag="ONT65_000446"
     CDS             65006..66025
                     /locus_tag="ONT65_000446"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2328100.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        22102 a        11476 c        13458 g        19012 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
    24061 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
    24121 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
    24181 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
    24241 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
    24301 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
    24361 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
    24421 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
    24481 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
    24541 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
    24601 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
    24661 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
    24721 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
    24781 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
    24841 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
    24901 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
    24961 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
    25021 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
    25081 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
    25141 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
    25201 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
    25261 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
    25321 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
    25381 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
    25441 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
    25501 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
    25561 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
    25621 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
    25681 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
    25741 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
    25801 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
    25861 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
    25921 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
    25981 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
    26041 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
    26101 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
    26161 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
    26221 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
    26281 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
    26341 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
    26401 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
    26461 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
    26521 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
    26581 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
    26641 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
    26701 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
    26761 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
    26821 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
    26881 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
    26941 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
    27001 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
    27061 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
    27121 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
    27181 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
    27241 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
    27301 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
    27361 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
    27421 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
    27481 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
    27541 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
    27601 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
    27661 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
    27721 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
    27781 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
    27841 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
    27901 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
    27961 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
    28021 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
    28081 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
    28141 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
    28201 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    28261 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    28321 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    28381 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    28441 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    28501 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    28561 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    28621 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    28681 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    28741 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    28801 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    28861 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    28921 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    28981 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    29041 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    29101 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    29161 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    29221 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    29281 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    29341 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    29401 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    29461 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    29521 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    29581 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    29641 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    29701 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    29761 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    29821 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    29881 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    29941 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    30001 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    30061 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    30121 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    30181 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    30241 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    30301 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    30361 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    30421 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    30481 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    30541 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    30601 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    30661 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    30721 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    30781 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    30841 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    30901 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    30961 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    31021 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    31081 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
    31141 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
    31201 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
    31261 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
    31321 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    31381 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    31441 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    31501 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    31561 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    31621 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    31681 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    31741 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    31801 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    31861 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    31921 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    31981 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    32041 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    32101 caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt
    32161 gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
    32221 acaccgttta ttcagataag ggactgtgac aagacttttg tcacagtttc tttttgttta
    32281 ttctttttta tctttgtatg gcaataatag acaatataac gaaaattatt taatggcttt
    32341 tggaagacca tggaaaacct tgtacaaagc tattttttct gaaaaattcc ttgaaacctc
    32401 acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt gttgattttt taataattat
    32461 tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc caataaaggg
    32521 gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg ccttgtactt
    32581 gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag cggttcagca
    32641 gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc tgacttatca
    32701 gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg aatttaccgt
    32761 cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga agtcagtgaa
    32821 gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa tggtgatcct
    32881 gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc tacagcttct
    32941 gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc tggaaagaaa
    33001 gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat caaattgacg
    33061 aaacaaacac catatttcca atatctgctt gctttcccat catttttccc acaaaaacaa
    33121 tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc tgtttataat
    33181 ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg gacttataag
    33241 aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa agtcagtgta
    33301 gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga tgacgtgatc
    33361 ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc tgttaaagaa
    33421 gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt caacaatgtg
    33481 aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa acaagtacta
    33541 ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa aagtccaaca
    33601 acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa agataaagca
    33661 aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga atttgatttg
    33721 atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa
    33781 gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt acgtcttgac
    33841 cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga ctatgcagac
    33901 ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc
    33961 aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg cgatgaacaa
    34021 gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt
    34081 ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac
    34141 catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata atcgttaata
    34201 ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc tcatattcac
    34261 ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta ttccggcaat
    34321 gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa attctggttt
    34381 tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc
    34441 cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc ataagaatag
    34501 atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta ttaaaataac
    34561 cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt gattgctcct
    34621 ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc aaaagctagg
    34681 gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt ttcatgatca
    34741 tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg ccgggcacgc
    34801 catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg
    34861 gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg cttcaaggag
    34921 attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta gctggaagaa
    34981 tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc ctagggacga
    35041 ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct tctacactcg
    35101 tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta caatatatct
    35161 ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg tacacaatct
    35221 taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt atccgtacgg
    35281 aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa ggattgagcc
    35341 gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg atgactttat
    35401 taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat atctttgcta
    35461 ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat cctacgatca
    35521 tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc gtggatctat
    35581 tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga taaatggaaa
    35641 tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt cttccattac
    35701 aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat tttatccaag
    35761 attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt ttactattgg
    35821 tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca actgcccaaa
    35881 acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat ggattgaatg
    35941 gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa
    36001 acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc cgcttattta
    36061 tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat gtaacgattg
    36121 gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac gtgatgcaac
    36181 gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg atgttggtcg
    36241 tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac tggataccaa
    36301 tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat gttttagctg
    36361 gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg ccgaatatct
    36421 ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc ttcgaagcct
    36481 ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga acgatgttga
    36541 gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg gcagtaactt
    36601 tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat gcctttgatc
    36661 ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact tagaaatctc
    36721 ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc atttgaataa
    36781 aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga cgaccagaag
    36841 catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg
    36901 taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta aagaaatagc
    36961 gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg ggaagcaagt
    37021 cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt
    37081 agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca gttatccgca
    37141 tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg tatgttatcc
    37201 ggaaatcctg attgctgatg aacctacaac agcgcttgat gtgactattc aagcacagat
    37261 tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct ttatcactca
    37321 tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat
    37381 cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct atacttgggg
    37441 actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg ctattcctgg
    37501 ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa gaaatgaatt
    37561 cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa aaacacacaa
    37621 ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag aagaaatcca
    37681 acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag gaggaattgc
    37741 tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa
    37801 agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga gaaacattcg
    37861 gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc attcgcttga
    37921 atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag atcaaagata
    37981 aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat ccatacgctt
    38041 cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat gttaatggac
    38101 tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga actgttggat
    38161 tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa cgccagcgta
    38221 ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat gaaccaattt
    38281 ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat ctccaaaaag
    38341 aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa catattagtg
    38401 accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt gatgaaatct
    38461 ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct ctaccagatc
    38521 cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat gacggacaag
    38581 tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag caggaagtcc
    38641 catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct aactaggagg
    38701 gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac aaaaacagca
    38761 attgattcgt acttttggat gtgctcgatt tacctataac catttgctga aaaagcggca
    38821 gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta cattaaaaaa
    38881 agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac agcttaattt
    38941 ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa
    39001 aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct atctggaaaa
    39061 tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc atcggccagt
    39121 tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat tttatgtcgc
    39181 actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc
    39241 ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt taccaaaatt
    39301 tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc
    39361 cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac aaaagagaaa
    39421 agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg aacaattatc
    39481 tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa aagaagaggc
    39541 acatgctgat ttttctattc acgactggca taaattaata acaaaattgc gttataagtc
    39601 ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag aaagcaattc
    39661 cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca agggataaaa
    39721 aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt cttaatgact
    39781 tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa accaataaaa
    39841 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
    39901 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
    39961 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
    40021 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
    40081 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
    40141 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
    40201 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
    40261 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
    40321 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
    40381 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
    40441 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
    40501 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
    40561 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
    40621 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
    40681 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
    40741 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
    40801 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
    40861 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
    40921 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
    40981 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
    41041 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
    41101 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
    41161 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
    41221 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
    41281 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
    41341 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
    41401 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
    41461 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
    41521 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
    41581 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
    41641 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
    41701 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
    41761 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
    41821 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
    41881 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
    41941 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
    42001 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
    42061 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
    42121 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
    42181 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
    42241 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
    42301 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
    42361 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
    42421 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
    42481 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
    42541 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
    42601 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
    42661 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
    42721 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
    42781 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
    42841 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
    42901 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
    42961 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
    43021 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
    43081 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
    43141 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
    43201 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
    43261 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
    43321 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
    43381 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
    43441 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
    43501 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
    43561 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
    43621 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
    43681 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
    43741 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
    43801 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
    43861 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
    43921 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
    43981 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
    44041 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
    44101 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
    44161 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
    44221 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
    44281 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
    44341 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
    44401 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
    44461 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
    44521 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
    44581 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
    44641 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
    44701 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
    44761 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
    44821 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
    44881 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
    44941 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
    45001 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
    45061 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
    45121 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
    45181 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
    45241 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
    45301 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
    45361 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
    45421 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
    45481 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
    45541 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
    45601 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
    45661 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
    45721 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
    45781 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
    45841 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
    45901 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
    45961 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
    46021 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
    46081 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
    46141 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
    46201 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
    46261 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
    46321 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
    46381 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
    46441 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
    46501 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
    46561 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
    46621 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
    46681 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
    46741 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
    46801 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
    46861 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
    46921 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
    46981 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
    47041 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
    47101 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
    47161 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
    47221 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
    47281 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
    47341 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
    47401 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
    47461 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
    47521 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
    47581 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
    47641 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
    47701 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
    47761 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
    47821 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
    47881 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
    47941 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
    48001 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
    48061 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
    48121 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
    48181 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
    48241 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
    48301 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
    48361 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
    48421 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    48481 agttcctcat gtttttcgtt tgtttgggct tgttaccgca gatgcaagct tttgaacacc
    48541 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
    48601 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
    48661 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
    48721 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
    48781 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
    48841 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
    48901 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
    48961 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
    49021 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
    49081 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
    49141 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
    49201 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
    49261 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
    49321 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
    49381 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
    49441 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
    49501 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
    49561 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
    49621 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
    49681 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
    49741 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
    49801 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    49861 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    49921 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    49981 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    50041 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    50101 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    50161 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    50221 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    50281 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    50341 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    50401 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    50461 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    50521 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    50581 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    50641 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    50701 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    50761 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    50821 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    50881 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    50941 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    51001 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    51061 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    51121 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    51181 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    51241 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    51301 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    51361 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    51421 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    51481 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    51541 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    51601 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    51661 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    51721 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    51781 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    51841 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    51901 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    51961 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    52021 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    52081 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    52141 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    52201 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    52261 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    52321 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    52381 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    52441 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    52501 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    52561 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    52621 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    52681 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    52741 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    52801 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    52861 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    52921 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    52981 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    53041 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    53101 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    53161 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    53221 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    53281 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    53341 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    53401 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    53461 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    53521 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    53581 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    53641 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    53701 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    53761 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    53821 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    53881 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    53941 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    54001 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    54061 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    54121 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    54181 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    54241 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    54301 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    54361 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    54421 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    54481 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    54541 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    54601 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    54661 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    54721 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    54781 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    54841 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    54901 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    54961 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    55021 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    55081 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    55141 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    55201 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    55261 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    55321 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    55381 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    55441 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    55501 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    55561 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    55621 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    55681 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    55741 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    55801 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    55861 ttagaaattg aatagactat tttttttaaa aaaaagaaaa tgagaaaaca tgccagtctc
    55921 ttcattcata ggaacgtcta ttttacttaa tttttctata aatgaattct gcataaaaaa
    55981 tatatcaagt aaatgtttat agatgaaact ctatttactt cttttttaat ttgttcttgc
    56041 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
    56101 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
    56161 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
    56221 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
    56281 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
    56341 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
    56401 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
    56461 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
    56521 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
    56581 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
    56641 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
    56701 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
    56761 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
    56821 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
    56881 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
    56941 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
    57001 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
    57061 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
    57121 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
    57181 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
    57241 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
    57301 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
    57361 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
    57421 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
    57481 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
    57541 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
    57601 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
    57661 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
    57721 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
    57781 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
    57841 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
    57901 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
    57961 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
    58021 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
    58081 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
    58141 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
    58201 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
    58261 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
    58321 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
    58381 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
    58441 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
    58501 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
    58561 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
    58621 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
    58681 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
    58741 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
    58801 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
    58861 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
    58921 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
    58981 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
    59041 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
    59101 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
    59161 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
    59221 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
    59281 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
    59341 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
    59401 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
    59461 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
    59521 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
    59581 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
    59641 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
    59701 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
    59761 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
    59821 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
    59881 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
    59941 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
    60001 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
    60061 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
    60121 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
    60181 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
    60241 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
    60301 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
    60361 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
    60421 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
    60481 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
    60541 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
    60601 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
    60661 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
    60721 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
    60781 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
    60841 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
    60901 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
    60961 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
    61021 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
    61081 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
    61141 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
    61201 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
    61261 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
    61321 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
    61381 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
    61441 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
    61501 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
    61561 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
    61621 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
    61681 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
    61741 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
    61801 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
    61861 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
    61921 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
    61981 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
    62041 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
    62101 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
    62161 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
    62221 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
    62281 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
    62341 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
    62401 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
    62461 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
    62521 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
    62581 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
    62641 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
    62701 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
    62761 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
    62821 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
    62881 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
    62941 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
    63001 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
    63061 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
    63121 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
    63181 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
    63241 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
    63301 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
    63361 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
    63421 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
    63481 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
    63541 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
    63601 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
    63661 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
    63721 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
    63781 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
    63841 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
    63901 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
    63961 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
    64021 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
    64081 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
    64141 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
    64201 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
    64261 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
    64321 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
    64381 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
    64441 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
    64501 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
    64561 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
    64621 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
    64681 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
    64741 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
    64801 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
    64861 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
    64921 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
    64981 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
    65041 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
    65101 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
    65161 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
    65221 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
    65281 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
    65341 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
    65401 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
    65461 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
    65521 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
    65581 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
    65641 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
    65701 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
    65761 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
    65821 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
    65881 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
    65941 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
    66001 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DAJSBC010000007        63477 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSBC010000007 DAJSBC010000000
VERSION     DAJSBC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.007"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..550)
                     /locus_tag="ONT65_000447"
     CDS             complement(152..550)
                     /locus_tag="ONT65_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR2328101.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ONT65_000448"
     CDS             complement(547..1320)
                     /locus_tag="ONT65_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2328102.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ONT65_000449"
     CDS             complement(1317..2000)
                     /locus_tag="ONT65_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2328103.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ONT65_000450"
     CDS             complement(2167..3054)
                     /locus_tag="ONT65_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2328104.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ONT65_000451"
     CDS             complement(3051..3920)
                     /locus_tag="ONT65_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2328105.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ONT65_000452"
     CDS             complement(4057..5343)
                     /locus_tag="ONT65_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2328106.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ONT65_000453"
     CDS             5616..6194
                     /locus_tag="ONT65_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2328107.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ONT65_000454"
     CDS             6194..7123
                     /locus_tag="ONT65_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328108.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ONT65_000455"
     CDS             7120..8856
                     /locus_tag="ONT65_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2328109.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT65_000456"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT65_000456"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR2328110.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ONT65_000457"
     CDS             complement(9752..10261)
                     /locus_tag="ONT65_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR2328111.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT65_000458"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT65_000458"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR2328112.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ONT65_000459"
     CDS             complement(11562..12653)
                     /locus_tag="ONT65_000459"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR2328113.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT65_000460"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT65_000460"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR2328114.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT65_000461"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT65_000461"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR2328115.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT65_000462"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT65_000462"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR2328116.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ONT65_000463"
     CDS             complement(16616..17590)
                     /locus_tag="ONT65_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR2328117.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ONT65_000464"
     CDS             complement(17571..19454)
                     /locus_tag="ONT65_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR2328118.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ONT65_000465"
     CDS             complement(19472..21697)
                     /locus_tag="ONT65_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2328119.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ONT65_000466"
     CDS             21812..22846
                     /locus_tag="ONT65_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2328120.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ONT65_000467"
     CDS             complement(22913..23926)
                     /locus_tag="ONT65_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2328121.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ONT65_000468"
     CDS             complement(24004..24192)
                     /locus_tag="ONT65_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR2328122.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24632..24760)
                     /locus_tag="ONT65_000469"
     CDS             complement(24632..24760)
                     /locus_tag="ONT65_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2328123.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="ONT65_000470"
     CDS             complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="ONT65_000470"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2328124.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26247..27302)
                     /locus_tag="ONT65_000471"
     CDS             complement(26247..27302)
                     /locus_tag="ONT65_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2328125.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="ONT65_000472"
     CDS             complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="ONT65_000472"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR2328126.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="ONT65_000473"
     CDS             complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="ONT65_000473"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR2328127.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="ONT65_000474"
     CDS             complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="ONT65_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR2328128.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="ONT65_000475"
     CDS             complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="ONT65_000475"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR2328129.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="ONT65_000476"
     CDS             complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="ONT65_000476"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR2328130.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35064..36623
                     /locus_tag="ONT65_000477"
     CDS             35064..36623
                     /locus_tag="ONT65_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR2328131.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36734..38665)
                     /locus_tag="ONT65_000478"
     CDS             complement(36734..38665)
                     /locus_tag="ONT65_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2328132.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="ONT65_000479"
     CDS             complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="ONT65_000479"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2328133.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39594..40346)
                     /locus_tag="ONT65_000480"
     CDS             complement(39594..40346)
                     /locus_tag="ONT65_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2328134.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40505..40714)
                     /locus_tag="ONT65_000481"
     CDS             complement(40505..40714)
                     /locus_tag="ONT65_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328135.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40725..42053)
                     /locus_tag="ONT65_000482"
     CDS             complement(40725..42053)
                     /locus_tag="ONT65_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328136.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42189..42344
                     /locus_tag="ONT65_000483"
     CDS             42189..42344
                     /locus_tag="ONT65_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328137.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42418..43005)
                     /locus_tag="ONT65_000484"
     CDS             complement(42418..43005)
                     /locus_tag="ONT65_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR2328138.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="ONT65_000485"
     CDS             complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="ONT65_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR2328139.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44585..45874)
                     /locus_tag="ONT65_000486"
     CDS             complement(44585..45874)
                     /locus_tag="ONT65_000486"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR2328140.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46028..46960)
                     /locus_tag="ONT65_000487"
     CDS             complement(46028..46960)
                     /locus_tag="ONT65_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328141.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47117..47776)
                     /locus_tag="ONT65_000488"
     CDS             complement(47117..47776)
                     /locus_tag="ONT65_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2328142.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="ONT65_000489"
     CDS             complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="ONT65_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2328143.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48817..49107)
                     /locus_tag="ONT65_000490"
     CDS             complement(48817..49107)
                     /locus_tag="ONT65_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328144.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49516..51321
                     /gene="glmS"
                     /locus_tag="ONT65_000491"
     CDS             49516..51321
                     /gene="glmS"
                     /locus_tag="ONT65_000491"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR2328145.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51393..52748)
                     /locus_tag="ONT65_000492"
     CDS             complement(51393..52748)
                     /locus_tag="ONT65_000492"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR2328146.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52772..53941)
                     /locus_tag="ONT65_000493"
     CDS             complement(52772..53941)
                     /locus_tag="ONT65_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328147.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53938..54825)
                     /locus_tag="ONT65_000494"
     CDS             complement(53938..54825)
                     /locus_tag="ONT65_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR2328148.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="ONT65_000495"
     CDS             complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="ONT65_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR2328149.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58905..59426
                     /locus_tag="ONT65_000496"
     CDS             58905..59426
                     /locus_tag="ONT65_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2328150.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60125..60682)
                     /locus_tag="ONT65_000497"
     CDS             complement(60125..60682)
                     /locus_tag="ONT65_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328151.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60861..61472)
                     /locus_tag="ONT65_000498"
     CDS             complement(60861..61472)
                     /locus_tag="ONT65_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328152.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61585..61842)
                     /locus_tag="ONT65_000499"
     CDS             complement(61585..61842)
                     /locus_tag="ONT65_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328153.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62439..62717)
                     /locus_tag="ONT65_000500"
     CDS             complement(62439..62717)
                     /locus_tag="ONT65_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328154.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62751..63002)
                     /locus_tag="ONT65_000501"
     CDS             complement(62751..63002)
                     /locus_tag="ONT65_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328155.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17689 a        13738 c        11220 g        20830 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattgat tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaaaag actaagaaac aataacaaaa
    24601 gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta gggccttttc
    24661 tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt
    24721 tctttttgat caattctttc attttttctt tttggtccat ttttattctc ctttaacgaa
    24781 tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct gaactttctt
    24841 cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg ccggattaca
    24901 tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa aatgatgatc
    24961 taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt tttcttgcca
    25021 ttttttcata actttttctg cttttccgac ttcataatga acattttcga ttttattatt
    25081 taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc catacacctt
    25141 atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag cgtcgatcac
    25201 aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat actctgcttg
    25261 ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc ctaatagctg
    25321 atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa tcacatttgt
    25381 tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt catgaatagc
    25441 ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa cgaccatcat
    25501 ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt catttttttc
    25561 ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt tgattgcttg
    25621 atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat ggctattctt
    25681 tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct gtgctttatt
    25741 acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt ctggcatctt
    25801 agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct gttgttcata
    25861 cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt ctacaggctg
    25921 acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact tattgcctgc
    25981 ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa ataatggata
    26041 accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt cgactgtata
    26101 tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta agcctatttt
    26161 tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc ggcactgacc
    26221 ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc gtctgctatt
    26281 tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac gaattctttg
    26341 ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg aatctgatcg
    26401 gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg tgcttccccg
    26461 ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc tgtttcaacg
    26521 aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg accgccattc
    26581 agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac aatgattaaa
    26641 gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc tccaacagag
    26701 ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc atcatacttc
    26761 aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc aagataagca
    26821 agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc tgtcaaatag
    26881 ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc catttttacg
    26941 gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg aatttttaat
    27001 gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg acggtgttca
    27061 agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc tgccactact
    27121 agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc tggttctggt
    27181 gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag gatatccgcc
    27241 aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg tgcttttttc
    27301 attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac gacaccagga
    27361 tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac cgcacgatct
    27421 tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc attaatgatt
    27481 ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac tacttcttgg
    27541 gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt tgagctgatc
    27601 gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa ttttgtctca
    27661 gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa ccagttagat
    27721 gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga catttctttt
    27781 gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat atagcgttcg
    27841 cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca ttcgtcatcg
    27901 atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga cccttctttg
    27961 acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt
    28021 tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa accttttttc
    28081 acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc ttcttgtcca
    28141 taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat tttcacatcg
    28201 ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta tgcttcttct
    28261 ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa
    28321 tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc ttctaaatgg
    28381 acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat ccatccgtca
    28441 tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc tgggtaaaag
    28501 taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc caaagctgct
    28561 ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg ataaccccag
    28621 tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc tggagagaaa
    28681 atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt ttcaaaattc
    28741 attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt gcttgttcaa
    28801 acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg atctgcaatc
    28861 caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt ccagccaagt
    28921 tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta atgttctcac
    28981 cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa tctgcaaaga
    29041 ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt
    29101 cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt acttcttcac
    29161 cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca ttttcagagc
    29221 gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa
    29281 tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct tccacaacag
    29341 cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg ccaggggcca
    29401 caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg tcgcctgtca
    29461 gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt tcgtcataac
    29521 cgctgatagc tgtcaatgct aatgcattat ctttcaccgt acgggtcatt ggtccgattt
    29581 ggtcaagaga tgaagcaaag gcaatcaaac caaaacgtga gacacggcca taagtaggtt
    29641 tcatccctac gatgccgtta aaagctgctg gttggcggat acttccccca gtatcactac
    29701 ctaaagaaac tggaatttgt ccagctgcaa ctgctgctgc agaaccacca gaagagccac
    29761 caggtacttt tgttgggtcc caagcatttt ttgttttctt gaaataagaa gtttctgtac
    29821 ttccacccat agcaaattca tccatgttta gcttaccaac agggatcata tctgcttgat
    29881 agattttatc catgaccgtt gcatcataaa ttggttcaaa attgtaaagg atcttagatg
    29941 cagcggttgt caagatatct tttgttacga tattatcctt gataccaatt ggaataccag
    30001 caaggacgtt gctttcatca atcccttttt catctaaagc ttttgcctgt tccaatgctt
    30061 tttcttcgtt caatgtaatg aatgcctcga cctcaggctc tgtttctttg attcgttcaa
    30121 atgttgtttt tgtcaaatct tgtacagtga tttctttcga tacaagcata tgatgcagtt
    30181 cttctagaga acgattgtgt aattctgtca ttatgcgcca gcctccccat tatcgatgat
    30241 tgcgggtact ttgataaagc cgtcttctgt ttccggcaca tttttcaata attcatcgcg
    30301 gttccatcca gcttgcgccc gatcttgtcg catcacgttt actgtttcaa ccacattcga
    30361 cgtaaatgga acaccttccg tatctacttc ttccagcatt tcaaccatat cgatgatttt
    30421 acctaattgg tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt
    30481 ggcaacgtgt ttcacttgtt cttcactaat cgccatacta ccccttcttt ctaaacgagt
    30541 taaaaacttt gctctctatt ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat
    30601 ttgtctgaaa aaactcattt ttcattcttc tactgagcga cttttagcca atgcatctgc
    30661 catttgatct tctgtccaaa cttcgattcc taattcttgt gctttggtca gcttgctgcc
    30721 agcatcttct cctgctacca cgatatccgt ttttttcgat acgctaccag tcactttccc
    30781 gccaagattt tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta gtttaccagt
    30841 aaggactact gttttttctt taaaaggaga ctcgacttct tgcaattggg catttcgaag
    30901 ccctttgtat tcgaaattga cgcctgcttg ttttaattca ttcatcaatt catggacttc
    30961 ttcgttactg aaataagtga ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc
    31021 aataatcgac tcatagtcac ttttactcaa tgtttctaga tcaccgaaat gttctgctaa
    31081 aatttttgct gctttcgctc cgacatgtcg gattcctaac ccgaaaatca accgctcgac
    31141 cgaattatct ttgctgttgt cgattgctgt caaaatatta tttgctgatt tttcttttat
    31201 tttgtccaac gtcaataact cttcttctgt caatttgtaa agatcagcaa catctgctac
    31261 tagtttttta tcatacatct gttctagtac gcgcggcccc aacccatcga tattcatcgc
    31321 atttcttgaa acaaagtggt tcaaaccttc tttcatttga gcagggcatt taggattgat
    31381 gcaccgtaag gctacttctt catccaaatg gacaagttca cttccacaaa ctggacaatg
    31441 tgtaggcaat tggtattctt cgctgtcttt aggtcgttta tctaacacga cttgcgaaac
    31501 ttctgggatg atgtctcccg ctttataaat cagaactgta tctttcaagc ggatatcctt
    31561 caacttaata taatcaccgt tatgcaggct ggcacggctt actgtcgttc ctgctactcg
    31621 gactggtgtc atgatagctg ttggcgttac tacccctgtt cgaccgactg tccattcgat
    31681 gttttcgatt agcgtttcaa cttcttctgg cggaaacttg taagctgctg cccatctcgg
    31741 tgctttgata gtaaaaccaa gttgatcttg tagcgagaat tcattcactt tgatgacgat
    31801 tccatcgatt tcatagggca aatccacacg tttgtcatga tattcttcaa tgtatgacca
    31861 cacttcatca atcgaatgac atagacgttt ttcccgattc gtgtggaatc caattttttc
    31921 tagctcttct agcgcatcat actgggtttt tgcttgcata ggaccgaaat cagctactgt
    31981 gtataaaaaa gtgtccagat ttcttttcgc tgtgatttta gaatctaact gacgcaaaga
    32041 accagctgcg gcgttccgag gattcgcaaa gatatctttt ccctctgctt ctctgtcttg
    32101 gttcaattgg acaaatgacc gttttggcat gaaacattcg cctcttactt cgatgttcat
    32161 tggttctttt aatttgattg ggaccgaccg gacagttttt aggttttcag tgatgttttc
    32221 tcctacagtt ccatccccgc gagtcgcacc tctcacgaaa tttccatctt cataacgcaa
    32281 agaaacggaa agtccatcta tttttagttc acagcaatag tcaactacac gtcccactgc
    32341 tttttgtaca cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt cgttcaaact
    32401 gtaaagcgga atatcatgag tgactttttc aaaaccttct aatacttttc cgcctactcg
    32461 ttgagtggga gaatcagacg tgatcaaatc aggatattgt tcctcgattg ctactaattc
    32521 tgcgtattct ttatcataaa catagtcttc cactgttggt ttatcttcta cgtagtattc
    32581 gcgagaccat tggttcagtc ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat
    32641 cgtcatttca tgttccatca tttaccctcc actactaagc tttttcaatt ggcgcaaagg
    32701 cagccaaaag acgttttact ccttgacttg ggaaagccac atctagttct agatccttcg
    32761 acgtcccgct tactcggacg actgttccta gaccccattt tttgtgtttg actttatctc
    32821 caggtttcca gctgttattt tcccctccag tagcactttt agagggaaca accgctgtct
    32881 ctggctgttt atagctatat gctgtttttt tagcgaatac aggcgttttt ttaggcgtag
    32941 gacgcatgcc ttcgatttcc agtaactcct gatcgatttc ttctacaaaa cgagaaggtc
    33001 ggttatattg cgtccgtcca tataatgttc tggagaaagc atttgttaaa tacaacgctt
    33061 cttctgctcg cgtgatcccc acatatgcga gacggcgttc ttcctccagt tcgctttctt
    33121 ccatcaatgc tcgagataaa ggaaaaaccc cctcttctag accaatcagg aatacaactg
    33181 gaaattctaa tcctttggct gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt
    33241 ctaaattgtc aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt agtttttctt
    33301 ctggtgcatc agcgtcttct tcattttgct gttcgaattg tttgtcgaat tcttgtgtga
    33361 ccgttaaaaa ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt
    33421 ttaagtcttc tagatatccg ctacgatcca aaacctcttt cgtcaattct gtcactgtca
    33481 agtatgggat catctgagtg acttcttgga tcatctctcc aaatgcacct aattgctgtc
    33541 ctgcttttcc accgatattt gccagatcca cattttgcgc tgcttcaagc agtggccatt
    33601 catgaagaga agcaaaactg cgtagttttt cgatagatcc tggtccgatg cctcgtttgg
    33661 gcgaattaac gatacgctca aaactgattg aatcttgagg atttaccagc acattcaaat
    33721 acgctaaaat atctttgatt tctttacgat cgtagaactt gtgccctcca accattttat
    33781 aaggaatatt tgctttcaac agagtttctt ccattacacg agattgtgcg ttcgtccggt
    33841 acaagatggc aaagtcgccg taattccgat gattgcttcg aatctcttct tgcatccggt
    33901 ctacgataaa acgggtttcg tctcgttcat tatctgcacg gtagtaagtg atcttgtttc
    33961 cttctttgtt ttctgtccac aagttttttg gttttcgatt gctattgttt tcaatgactt
    34021 ggtttgctgc gctaaggatg tttttcgtgg agcgatagtt ttgttctagc aaaattactg
    34081 cagcatctgg ataatctttt tcaaaatcca agatgttctg catatctgca ccgcgccagc
    34141 cgtaaatact ttgatctgca tctcccacga cgcacaggtt cctaaatcta cctgccaata
    34201 aattgactag cgtatactgt gcgtgatttg tatcttggta ctcatctaca tgaatataat
    34261 ggaatttgtt ttgataatac gtgagtgaat caggatgttc ttcaaataat cgaatcgtat
    34321 tcatgatcag gtcgtcaaag tccatacatt gattatattg aagctctttt tggtaagcag
    34381 cataacattt tgccgcaatc tcttcgtaat aactgccttg catttttgca aaatcctgag
    34441 gggtctgcag gctgtttttt gcttgagaga ttgttcctaa tatacttcga ggatcatatt
    34501 ttttcggatc aatgttcagt tcttttagta tccgtttcat caaggtcagc tgttcagaag
    34561 aatcgatgat tgtaaagttc cggttatacc cgataaaatc aacatctctt cttaaaatac
    34621 gcacacacat cgagtgaaaa gtcgataccc agacatcttc gcctccagat gcgagaatcg
    34681 cattgacacg ttctttcatt tcttttgcag ccttattggt aaaagtaatc gctaggatgt
    34741 tccaaggatt gacttctttt tcctcaatca aataggcaat gcggtgtgtc agcactctcg
    34801 tctttccact tcctgcacct gccattagca gtaatggtcc gtcagtgtga agaacagctt
    34861 ctttctgacg ggggttcatc ccgtttaata attcagcttt ggatgacatt aacaacatcc
    34921 tttcttttat caatcttgtt aattatagca ttttcttggc tttcattaaa ctagctagtt
    34981 caaaatcatt tgtttctttt tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa
    35041 ttaaaagaaa gcgaggcgtt tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga
    35101 ctcagatata ttttattcta aaaggaatat ttgttggact gctgacagga atcgtcgtta
    35161 gcctgtttcg attatctatc gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga
    35221 gtcgggaaca gccgatttac ttaattggcc tagctatttg ctgcatcctt gcagcatttt
    35281 tcgtgggtta tctggtcaaa aatgaaccgg atatcaaggg aagcgggatt ccacaagtag
    35341 agggacagct tcgcggggaa ttatcaatga actggttctc tgttttatgg aaaaagttca
    35401 ttggcggtgt actttcagtt ggtgcgggcc tttttctagg tagagaagga ccttctatac
    35461 agcttggcgc atccattgga caaggagctg gacaattgtt tcggagtcca tcttcagaag
    35521 aaaagatttt aatttctagc ggtgcaagta gcggacttgc agcagcattc aatgctccta
    35581 tcgcaggatt actctttgtt ttagaagaaa tccatcactc cttttctcca cttgtttggc
    35641 tgacatcatt tgcttcagca atcactgcta acttcgtctc tctttacttt ttcggcttga
    35701 gacctgtttt atatctaggt gaccttcctt ctttgccgtt gaaatattat ggttccttag
    35761 tgatcttagg agtacttcta ggcatcttag gttttattta tcagaatgtc ttgctggctt
    35821 tgccaaaatt ttacaaccga ttgcctttac cagcttattt tcacgggttc gttcctttct
    35881 tgctgatttt gccaattggt tatttatggc ctagtactct aggcggaggc aatcaaatcg
    35941 ttttagcttt tggacatgct tccttgccaa tcattatgat tatcggtttg tttgttcttc
    36001 gattcgtttt ttccatgatt tcttacggtg ggggtcttcc aggggggatt ttccttccga
    36061 tattgacatt gggggcttta atcggtagct tttatggaaa cattatggtt gatttcggga
    36121 tggatccaat ctacatcaag gattttgtcg ttattgccat ggctggttat ttcacagcga
    36181 ttggaaaagc accgctgaca gcaattattt tagtgacaga aatggttggc tctcttaatc
    36241 atctgatgcc tttaggattg gctgctcttg ttgcttatat cgtcaatgac cttctcggcg
    36301 gtaatccaat ttacgaatca ttattggaac gtctacttgg aaatactcag accgcctcac
    36361 taaaaggtgc aaggacaact tttgattttc cagtaacggc agagagttct ttagacggat
    36421 taatggtacg agacttcaat tggcctaaaa atatgctgct aatctctata cgcagaggaa
    36481 atcaggaaat cttgactcac ggtgatacga tcatgaatgt aggagatatt ttggttatct
    36541 tgactgatga agcaaacttg ccaagaatca agcaggaaat cgaggctaaa tcttctatca
    36601 caaatttgaa gcaatcattt taacgaacat ggaaaaacta ttttacaaca actattcatc
    36661 tacactactt gattctatat gattctataa aaaaagaaga ggctgggaaa acccagcctc
    36721 ttctttttta tttctatgct tctgctactg caggagcttt tctcaataaa ccataaatca
    36781 caccagatac gactgctccg atagcaataa acaccaaata catgaccggg tgactcagta
    36841 agaatacaac gaaaatcccg ccgtgaggag ccagtaattt gattccgaaa gctccgacta
    36901 atccgccagt taaagcagaa ccaataataa aacttggaat ggcacgaatc gggtcagctg
    36961 ctgcaaatgg aatcgatcct tccgtgacaa acgatagtcc catcacgatg ttcgtcaaac
    37021 cagcatcttg atctgttttg ctgaatttat tcttaaacaa acgtgtagca acgaaaattg
    37081 ccaatggagg taccattcct ccagccataa cagaagccat cacgacactt ccgccagttg
    37141 caacagtcgt agctaaagtt gctgtaccaa acacataagc ggctttgttg attggaccgc
    37201 ctaaatctgc cgccatcatt ccagctaata atgcaccaag taaagcagca ttcgaaccac
    37261 tcatacttgt caagaaaccg ttcaatgcat cattgattgc tttcataggg acattgacca
    37321 gcaacatcaa gaaaccagta atcagtaagc caaacactgg ataaaagagg atcgttttaa
    37381 ttccatccaa tgattttggt aagttcttga ataatttacg taaaaatacg atgacataac
    37441 cagctaagaa tccaccaatc agtgcaccta agaatcctgc accgcctgta ttagctaatg
    37501 caccagcagc aaatccaacg atcaaaccag ggcggtcacc gatacttgaa gcaataaatc
    37561 ctgctaatac cggcagcatg aaaccaaatg cagcttgtcc gatttggttg aaccagctag
    37621 cagcttgatt gtaattccct agatttgata attgatcatg tggcacgcca ataaattggt
    37681 cgatcatgaa agaaagcgca atagcgattc ctccaccaat aacgaaaggt aacatgtgtg
    37741 atacgccgtt catcaaatct ttgtaaattc tttgtccgat agtaccatcc gcatttcctt
    37801 cttctgaact gctttctttt ccatcaccat ggaatactgg tgcagtacca ctaagggcta
    37861 gattgatcaa ctcttctggt ttacggatac catcactgac aggacgattg atcagttctt
    37921 ttccgtcaaa gcgattcatc tctacttttt tgtctgctgc aacgattaca ccttctgcac
    37981 gggcgatgtc ttcagcagtc aatcggtttt tgattccttc tgaaccgttc gtttcgactt
    38041 tgatctccac acccatttct ttcgcttttt tcttcaaagc atcttctgcc atgtaagtgt
    38101 gagcgatccc tgttggacaa gctgtaacgg caacaacgaa ttttctttct ggatgagcag
    38161 aagcttctgc agcttgtgtt tcttgttctg ctttttcttc tgcttctttt tgctcttcag
    38221 ctgtttggaa caagtcttgg acttcttctg gtgtatttgc ttcttttaat tttcctacga
    38281 aatctgcatc aatcagcaat cgagatagtg ctgctaatgc ttgcaaatgt gtgtcgtttg
    38341 cgccttctgg agctgcgatc atgaaaaaca gataagttgg ctgtccgtct aaggcttcat
    38401 aatctactcc tttttggctt tttgcgaata aaactgttgc ttctttaact gcactgtttt
    38461 tcgcgtgagg catggcgatt ccatcaccaa gacctgtaga agtttgtgct tcgcgggcta
    38521 gaattccttc tttatatgtt tcgatatcgg aaatccggcc ggcatcaaac attttttgga
    38581 ccatttcatc gattgcgcct tttttatcag tcgcttgcaa atccatgatc atcgcttctt
    38641 tgaccattaa atccttgata ttcatgatta tcctccttga aactaatcaa tttttgtgat
    38701 ttcaacttct ggcagcaact catcaatcaa ttcgcgagct gccaagtcat cagaaaaagc
    38761 tgttgcgctt ccgcaagcaa ctccccattt aaaggcttcg attggatctt tcgtacggct
    38821 gtaagcccca ataaagccag caatcattga atcccctgca cctactgaat ttttcaatgg
    38881 gcgaactaat acattcgttt tataagttcc ttgttccgtg aagagcaaag caccgtctcc
    38941 agccattgag atcagcacat tctgtgcccc ttcagctagt aattttttcc catacggaat
    39001 gatttcttct acagattgga aggttgtgtt gtaaagctca gctaattcgt gattgtttgg
    39061 tttgatcaac aacggtttct taacaagtgc attttttagg tcatcacctg ttgtatcaat
    39121 cacgaaatct gcaccttttt ctcgaatgat atcaatcagt tcttgataaa atccttttcg
    39181 caggctagct ggcgcgcttc ctgacaatac gacgatatct ccttgtccta ctgttcctaa
    39241 tgcttgcttc aactcatcga tttcttcatc tgaaatagat gggcctaagc cattgatctc
    39301 agtttcagtg tctgatttta gtttgatatt gattcttgta tcttctttta cggttgtgaa
    39361 caaagactgg atttgttctt tttcaagcca cttcgaaata aattcgccag tgaaccctcc
    39421 taaaaaccct agtgctgtag attgagaatc gatccgtttc aaaatacggg aaacattgat
    39481 cccttttcct ccaggtaact tgaagtcttc cttcattcgg ttcaaatcgc ctagttttaa
    39541 cccatctacg cgtacgatat aatcaattga tggattaagt gttactgtgt aaatcattgt
    39601 attgcctcct taattgttgt cttttcacgg aattcattaa cgaattctaa tggacaacgc
    39661 tcggttatga tcgtggctgc ttccaaaggt gcaacagagg tgaaggacaa ctggttgaat
    39721 ttggtatgat caatcaatac aaatgtctct tcggaatttt tcatggctaa acgtttaaca
    39781 gctgcctctt ctggatccgg agtcgtgaat ccttgttcaa gatgggcacc gttcattccc
    39841 ataaatgctt tattaaaatg aaaatttttt agttgttcta tcgcattaga tcctaaaaca
    39901 gcattagttg ccagctttat cgttccacct aaaataatgg tagcaagctc caaatcaact
    39961 aaatttgccg catgttgaac ggaatttgtc actactgtga tctgttttcc ttgcagaaaa
    40021 ggaatcattt ctaacgtagt agagccagcg tctaaataaa tgacatcttc gtcattaatg
    40081 gtttcagctg ccgatttggc gatttgctgt ttttcgtgaa cgtttttgag tgatttttca
    40141 ctcatattct gctcaaaatt caataattgc tctttcttag ctccgccatg aactctttct
    40201 agaaacccac tattctccag ctcttgtaaa tcacgtctta tcgttgattc agaagcattc
    40261 aaaaggttta caagatcttg tgatttcacg attttctgct gttcaagcaa gtctaggatt
    40321 ttttgtctgc gttcctctgt aagcattttc acgatcacct caatacaaat aatagcatat
    40381 tttttttcta ctagcaatat aaatcagtca aaaacgttca taaaaattat aaatcagtca
    40441 aaaaaaaata aaaaaccagt cagaaagact ggttttttat ttttttgtct atcatcaagc
    40501 cgttttatcg ttttttattt tcgcttcttg tttttttcat ataaacagaa gcaggttttc
    40561 tttccttatg aagaagataa taataaagtc ccatcccgcc aaacatgacc aaaaaaccga
    40621 tgactgatcg aataggaaaa ttaggaacaa aaaagagaca gacggataat aatgatgccg
    40681 gaaccattat tcgataatag agttgtttat ccattttctt cacctcatat tttttgtaaa
    40741 gtaatcaatt gttctaattc tcggtaatct aaaaattgca cacgcagatg ctgctcacct
    40801 ttcttgacat cgaattggat acttcgccgc tttttcttta ttcgtttgaa ggtaacatca
    40861 gagagtctat gtttgatttc ctttaacggt aattctgaca gggattctga aggaacatag
    40921 attcttaagg ataaaagata ctcttgatat ttttcatctg ttacctctac ttctagatat
    40981 tccctaatat aataacaata agcttcgatt ccattatcca aagcaacagt cggatcgata
    41041 actttatagc acatcatgcg tgatcgattc tcgtcaaccc aaggaaaata cgcctgagca
    41101 tttagtgttc cgcgaacacg cctttcttta taatctcctt gttggatatc ataacttttt
    41161 gttacaaaaa atgaagcatt ctttttagga acaggttgca tttgtgcatt ctgatactgg
    41221 acagattgga tcgtatggtc agtgtaaatg tattcaacga atggcggttt caataaaccg
    41281 acaccattgt cccaagcaag atgatcaaac ccaagctggt ccctaaaata ggattgaaac
    41341 atttgattgt ttggcttttg ccagttaaag gggtctcgat aaagctggat acatatgact
    41401 tcgttttcta attccttcac tttttctact tgttggatgt cgagaaaaat cggtttaggg
    41461 atgatttgat gataatagcg actataatac atttcccaac acgaagtgaa acataaacct
    41521 tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga ctactacttc cccgttttgt
    41581 tgtttcattt taggtaaatg ttcgatttca tcctttttct caaacaatag ccgttgagag
    41641 atctgttttg tattgtgata gataaactcg ctataagctc gcatataggc gaacacccca
    41701 tgagcaatca tttttttctg aatgaagtct cgaatcaaaa attgcttttc tttgaaaaca
    41761 gagatagaca atacattttt tatcaataat tgattatctt ccaagcgaaa actacattcg
    41821 ttctcgtcat ttcctacaac taaccaactt tcgtctctta ccgatgctag cattattttt
    41881 tcttgaagct ttcgaagatc atacggatcc gcttgttgtg tgaaagagcg gataaatgtt
    41941 ggctggatca acagacgtgt caaaatagcc caatcgctta tttcctgttt tttttcttct
    42001 tcatttaagt ctgtgagctg gatagtcatc aataatttat cttcgccaat cattttctca
    42061 cctctttcaa atctttttat aatttaatta taaccaatga taaagaaaaa ctaaaacttt
    42121 atgtcttaga aaccaatttg gacgattaca tgatagacta agtaaaaaga aacaaagagg
    42181 tgtttacaat ggggttgaaa tttgaacgta cagataattt agataaactt ttcgaatcat
    42241 ttgctgtcga tccggaaaaa acagttgcac caaaagaaga gatcgatcga tttctaaaac
    42301 cacagccaca aaatgaaaag aaaaaagaca agaaacaaaa gtaagttaag ctcaaaaaaa
    42361 gacttaccag ccaaactttt atgaaagagt ttgactggta agtctttttt attcttttta
    42421 tgattcaatt gcttgttcga ttgctgccca cgcttcttct tttccctgtt ttgtaacgga
    42481 agaaaagaga ataaacgtgt cattgcgatc aaaatccaac gcttttttga tttcgctctc
    42541 atgcttgttc catttaccac gagggatctt atctgcctta gtggcaacaa caattacagg
    42601 gatatcataa tacttcaaaa attgatacat ctggatatct tcttgagaag gcttatgacg
    42661 caaatctact aaagaaacga ccgcacgtag ctggtctcta gtcgttaaat atgtctcgat
    42721 catctttccc cattttgctc gctcagtttt ggatactttt gcatacccat atcccggaac
    42781 atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc aatgtctggg tctttcctgg
    42841 cttaccggaa gtacgggcta aattctttcg atcaatcaat gtgttgataa aagaagattt
    42901 ccctacattt gatcgtcctg ccagagctat ttctggcaat tgtgtatctg gatattgttt
    42961 tggtgccact gcacttatga taatttcagc ttgatggact ttcatggtct ctctccttaa
    43021 caaggtgatg aataaagcaa gcggctgtcg caaaagccaa aatgactttt acggcagcct
    43081 cttttcatca acccgctttt ttctctgaat ctttatagat tactgttggc tcaccagaca
    43141 attcagctgc ttcttttgtg ataatcactt tttcgatgtt ttcatttgaa gggatatcaa
    43201 acatcacatc catcatgatt tcttcaatga tggaacgaag tcctcgagca ccggtattgc
    43261 gttcgattgc ttttttcgca atggctctca acgcttcagg ttcgaattca agttcggtat
    43321 tatctagaga aagaagtttt tgatattgtt tgactagcgc atttttcggc tcagtcaaga
    43381 tccgtaccag atcatctgtt gtcaatttct ctaaagctgc cataacaggt aatcgtccga
    43441 taaactcagg gatcaatccg aattttagta aatcttcagg aatgatatgc tgcatgacac
    43501 tttcgtcttc agaaagtttt tggttttgtg taccaaatcc aatcgtcttt tcgcccatac
    43561 gattcttcac aatcgtttcg atgccatcaa atgcaccacc tacgatgaac aatatgttcg
    43621 tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg tccgccctga ggtggcacac
    43681 ttgcaacagt tccttctaga attttcagca aggcttgttg gacaccttcg ccggaaacgt
    43741 cacgagtaat agatacattt tcgcttttac gcgcaatttt atcgatctca tcaatataga
    43801 tgatcccttt ttctgcaagt tccacattgt aatcagcaga ttgaagcaat ttaagcaaaa
    43861 tgttttctac atcttccccc acataaccag cttctgtcaa acttgtcgca tctgcgattg
    43921 caaaaggtac attcaatgtt tttgctaatg tttgtgctaa aaatgtttta ccagaccctg
    43981 taggtccgat caagcagata ttgctttttt gcagttcaac atcatctgtg ttttcttgtg
    44041 cttgctgatt gacacgttta tagtgattgt atacggcaac agctaacgag cgttttgcgc
    44101 gatcttgtcc aatcacgtag ttattaaata cttcaagaat ttctagtggt ttagggactt
    44161 cagtgaattc gcgtacagct tcttcataaa attcttcatc aatgatttct ttacataggt
    44221 cgatacattc attacagatg tagacccctg gaccagctac gatctttttc acttcttctt
    44281 gggttttccc gcaaaaagaa cagcgtacgg tctcattgct acttggattg tcatacatgg
    44341 tcttcacccc ttaacttgca aaaagtatgc aagaaactat caaagacttt acagtctttt
    44401 actatcataa catagtttag taaaaagaaa aagtgtttaa caaaggatca actgacctat
    44461 acctttgcat tttttactct taacaaaaaa taaggaactg tgacaaaatc gtgtcacagt
    44521 cccttatttc gaataaacaa ttttctagaa gtagcttctt cagaaatgat ttattacttg
    44581 atttctattc tttcgcttct gtcgctgtac ctgtgatcaa ttcgattgct tttttcattg
    44641 tcacatcatg ttccagcata tcttctgtca acacgcgttt gatttgttcg attggcatgt
    44701 tgtatgtttc tgataattct tggatttctg cattgatttc ttcttctgtt gcttgaagat
    44761 tttcagcagc tgcaattgct tcgatcacaa gatttgttct tgtgcgcatt tctgcttcac
    44821 cttcgaattg tttatgcaag tcttcttctg tagaaccagt taattgatag tacatttcag
    44881 gagagatacc ttgacgttgc atgttgttca agaattcgtc cattgaacga tgtacttcat
    44941 catgaaccat cacgtgtggc aattcaacga tttctgcgtt ttcaacagcc atacggatcg
    45001 ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt ttctactaat tctttgcgat
    45061 atttttcttt caattcgtct aatgttgata cttcgtcatc aacatctttt gcgaattcgt
    45121 catctaattc aggcacttct tttgctttta cttcatggat tgtcactttg aagactgctt
    45181 cttttccagc aagatcttct gcttgataat cttctgggaa agttacattt acatctagac
    45241 tttcacctga tttatgacca actaattgat cttcaaaacc tggaatgaat gagcctgaac
    45301 ctaattcaag tgagtaattt tcgccttttc cgccatcaaa cgcttcgcca tctacaaagc
    45361 cttcgaagtc gattactact gtatcgccat tttcagctgc agcatcttct ttgataacta
    45421 attcagcttg tgcttcttgt tcacgtttga tacgtgcatc cacgtcagca tctgttactt
    45481 cacggtcttg tttttcaaca actagatctt tatattgtcc taattttact tctggtttca
    45541 ctgttacttc agcagtgatt acccagtctt gacctttttc catgctttca acatcgattt
    45601 ttggttgaga aacaggatcg atgccagctt ctttgacagc agcttcataa gcttctggta
    45661 atacagcatt caatgcatct tcatataaag cttcttcacc gtacatacgg ttgaatactt
    45721 gacgagaaac tttcccttta cggaatccag gtacgttcaa gctttttttc actttattaa
    45781 acgcagttgt taatcctttt tggatttcgt cttgcgcaat cgtaaatttt aaaacaccat
    45841 cattcgtgcc ttttttttca aatgttgcag tcatttaatt ccctccgagt ttagaaattc
    45901 attttataca tcaaaaacgt ttcatgcata cctttaaata gtacactaac cctttcaaat
    45961 tgtaaacata ttgaatggat agaatccttg tttttcataa catttttatc tttttcactg
    46021 ataaaatcta atggaagaag atcatttgct gcaaaatttt ttctcttttt tttaaaaaat
    46081 cttgtttttc atcttctgta ccttcataca tcgtttttcc tgtacacgcc tctacagtca
    46141 gttgaaacca gcgcttcggg tcttcgatgt atgtatcata gacaggataa acaattgcag
    46201 attgcaacgt gaattcttct tttaacacgg aactaagcag cgcatcttca ttcccgtaac
    46261 gatctgaaaa tacacataat tgctggtaac ttgcctgctg ctgcggcttt ggcagatctt
    46321 ttggtatgac tttgactaac ttttcttcaa tcgtcaaata gaaaacagga tggcattcgt
    46381 tcaattgcct aagtgtctcc agtaattttg cccttgctaa aatatggacc tctggcgata
    46441 caataagttt actcgctgtt tggataaact catgataagg aagctgttcg atctcatcaa
    46501 tagacgcaag ctgacgtgca ggttccaacg aaggaagttc ctccgattta ttgtggagtg
    46561 ccttgatctt ctgcctctcc atttgtccgg ataattcttc tgcaaattgg attttttcaa
    46621 tgacccgctg ctcgaaagct tcataaaaat ctgtacttgc tatcaatttt ctagcatata
    46681 aaaaatcttg tgtactgatt aacaagtcaa aataaaattc agcaaactcc tcattttgaa
    46741 gatactgttt ttcatgcaag agtgcttgct tcaatgcttc ttttttctcg tccaactcgt
    46801 aaagacaaaa aacaagcagg ctattagcct caaagctttc tttcaactcg taagctcgtt
    46861 gaaataattc ttttgcctgc tgatattgct ggtttgctag tgcttgttgt gccactcgca
    46921 ttaaattctc atatttttct ggaaaatcaa tcggcttcac gcagtgtcct cctcttgctc
    46981 atattcagct catgttcaat agtagcaaaa caaaaaaagc atgacaatta aaaataactg
    47041 aagcaccctc taaaagctag attttagatc tgacttttag aaggtgcttt caactgttac
    47101 tgctttttat tgctcatcag ctgttgactt cctggttgaa cgctttgatc agattgatcg
    47161 taacaacact tgtattagtg aaaaaatcgc ccagatgttg ctttttctga ttaaagactg
    47221 ctggaagata ccctaacatc agcaaaggaa attttagaat gaaccggcaa gcaccttctc
    47281 taactaaaac ggttgaccag ctaagttctt cttcatcaaa gctgatgact cgaataccaa
    47341 agatcatttt cccaatcgtt tggccatgat tgaatttcgt caataaaata aaatacgcta
    47401 gataaataag taaggctgtg aatccgtaga gactcagata agaatctgaa aaattaagcc
    47461 aacctagatt tttggcaata ccaagtgtta aacgcgtaat agctccgata cacaaaagat
    47521 cgaccaagaa ggcaaacatt cggataaaaa aacctgcata gaaatactta ggaaaatcat
    47581 ttgcttttgg cggttcattt cttgtgtccg tatacttttt ccatttacgt tgtttctttt
    47641 tgatttcttc gggcgtcaaa ggctctcttt caaaagagct cagtaccttt tgggaaaagt
    47701 tggagtcagg tcctacagca ggctgctgtt gcttatcttt ttcttgttcc ttattttcat
    47761 ttgaaagctc tgacatgctt actcacctcc gtaataatac attgcttttg gtgattccgc
    47821 tgtccctaga ttctcaagaa cggataagat ctgactagtc tcagaaggct tcaatccctg
    47881 gaattctgct agtttacttc ctaaccatgt actagcaaac ccagatggcg tggaagaata
    47941 ttctactagt tccgcatctt caagctgttg ttcctttcgt atagctttta aagcatcttc
    48001 aggaaatccc aattcatcga ctaaacctac ttcttttgct tggacaccat cataaatcct
    48061 tccatcagca atttttttga cttcttcttc agatttattc cgtccctcgc tgacgattgc
    48121 tacaaaacga ctatatgcgc tgtcgatata tgcttgaagc accgcttctt cttcttttgt
    48181 ctctggtcgc gttcctgaac ccatatcttt caacgctccg cttttaactg ttgtatcttc
    48241 cacacccagt ttttctagaa gtcctgaata attcaatcct gacatgatca caccaattga
    48301 gccggtcact gtttcttctg ttgcaaagat tttatctgct tgagcagaaa tatagtagcc
    48361 accgctagct gccatgtttt tcatgctgac atacattggg atatcatgtt ctttacgaat
    48421 cgtatccaat ttcttagcga tttccgcact ttcgtagaca ccgccaccag gagaatttac
    48481 ttctaaaaag atacctttga ctgttggatc ttcttcaatc gccttcagtt gttccataaa
    48541 atttttatga ttgtaacctt cagaagcaaa taaacctgaa gaccctcccg cagcaatcgt
    48601 tccatctact gataatttta cgattttttg cgtactgtct ccctcttcca acacatttgg
    48661 agctagttct tcatccccat ataaccaact gttcaatccc tcaatttgat cgttttcctg
    48721 aggcttagtc aaactagctg aaactaacga aaccacaaat aatcctgcag caatcagaac
    48781 tgctgcccaa cgtcttttat tcattctttc tcctttctaa tacaagtttt ttatgacgat
    48841 cacagtttct gactgctcag aagtgtcgaa ccgctctgct ttttcttccc gcgcataccc
    48901 ggaaaacgac tcttttgtta attcatcttc taccgtaaac tcacgagctt gagcaacttc
    48961 caaggcatca ggtgcgattt cttggatgct atcccagcca gtatctaaaa atattttcgt
    49021 atcatctgat acaccagctg atgcttcgaa tttggacaat tgttttttta acattcccag
    49081 tgtcatgatc tcaatttttg aatccatttt tactcctcct ctatccgatt actttctatt
    49141 gtaccgaact cctgaaaaag atgatagata aaaacccatt attttttgac ataaaaagga
    49201 catttgacaa agaatttgat atataccaat ttatttttta ttcttttttt gttgacaaga
    49261 acattcgcaa aaggttatga ttttaaatgt ttccagataa aaaacaaaaa ggaggatagt
    49321 tatacctttt aggatagcgc caggtctgtg taaacagatg acgaggagag ggtttatcga
    49381 aagattcggc ggatggccct agggactgca ctctacagaa aatccacaaa aaatgtctgc
    49441 aaagataaaa caaaaggatt ttcctgcagc tggaagcatt catacgaatt cattatagaa
    49501 agtaggatgt tttttatgtg cggaattgtt ggaatgattg gattaaaaaa tgtaacacca
    49561 ggattgattg acggtctaga aaaattagaa taccgtggtt atgattcagc tggtatcttt
    49621 gtctcagatg gcacaactga ttatctggtc aaagcacaag gcagaattca aaatttgaaa
    49681 aacaaaataa atattgatac tacaggaaat ataggtattg gtcatacacg atgggcaact
    49741 cacgggcaac cttctgaaga aaacgcccat ccacatactt cccaaagtgg acgcttcgtg
    49801 ctcgttcaca acggcgtaat cgaaaacttt gaagaactta aaattgctta tttagcaaat
    49861 gaccacttta ttggtgagac cgatactgag attatcgctc atctaattga aactttcgcg
    49921 aaggatacta caacgcaaga ggcgttttta aaagctttgc gggtcatcaa aggttcttac
    49981 gctttcgctt tgattgatcg gacagcacct gatgttattt acgtcgctaa aaataaaagt
    50041 ccactattaa tcggactcgg cgatggattc aatgtgattg ccagcgatgc aatggctatg
    50101 cttgctcata cgaaggaatt cgttgagatt gaagatgaag agatggttac cgtcacatca
    50161 gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag aacgttcttc attcgaagca
    50221 caagtagatg cttcagatat cgagaaaggt acctatcctt tttatatgct gaaagaaatc
    50281 gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat acgtaacaga agacaatcgt
    50341 gtctctcttg atcaaaagct ggtcgataca ttgtctgata gcgatcgaat ctatatcgtt
    50401 gcttgcggaa ctagttacca tgcggggtta gcaggtaaac agactttgga aaaattgaca
    50461 cagattcctg ttgaggttca tttagccagt gaatttggct ataacacacc tcttctttca
    50521 aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa cagcagacag ccgccaagtg
    50581 ctggtaaaaa tcaaccgtct cggctaccct tcacttacga ttacaaatgt tgctggttct
    50641 actctttcgc gggaagcatc ctttacactg ctattgcatg ctggacccga aatcgcagta
    50701 gcatcaacga aagcttatac tgcacaaata gctgtactaa ctttattagc caaagctatc
    50761 ggtgataaaa aagaatttga aacttctctt gtattcgata tcactcatga attgagttta
    50821 gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt atttagaaga gttggcagaa
    50881 gtatatctca gcgatacaag aaatgccttt tacattggac gtggtgctga ctacaacgtt
    50941 tctcttgaag ctgctttgaa attgaaagaa atcagttata tccaggcaga aggttttgcc
    51001 gctggcgaat tgaaacatgg aacgattgct ttgatcgaag aaggaacacc agtgatcgga
    51061 atcatcagtg aagaagtgac tggtgcgcat actcgaggaa acctgaaaga agtcgaaagc
    51121 cgtggcgcta aaacactagt tattgtctca gagggattag aaaaggaaag tgaccagctg
    51181 gtgctaccaa ctgtccaccc ttatctaact actctaacca cagtcgtacc gactcaatta
    51241 cttgcatatt acgcaacgct attacgggga ttcgacgttg acaaaccacg taatttagcg
    51301 aaatcagtga cagtagaata ataaaagaac aaggaccagt gtcagacatc tgacactggt
    51361 ccttgttctt ttatttttta tgaaaatatc tcttaatcca gtccgatttc ttctttgact
    51421 actgccgcga ttttatcgac atagtaatta actttctcat cagtaggcgc ttctgccatc
    51481 actcgaagta atggttctgt accggaagga cgaactagga ttcttccttc tgatcccatt
    51541 tcagcttctg cctcttcaat cacttttttg attgccggaa catccatcgc accgttttta
    51601 tcacttacgc ggatattcac taatttttgc ggatagattg ttacctctgc tgcaagctct
    51661 gataatttct tacctgtctg tttcatgata ttcagtaact gaattcctga aagcatccca
    51721 tctccagttg tgttaaaatc taagaaaatc atgtgcccag actgctcgcc gccaaagtta
    51781 taatcgtttt tgcgcatttc ttctacaacg taacgatcac caacttgtgt aacgacatct
    51841 ttcaaaccga ttccttccac cgctttgtgg aatcctaagt tactcatgac agtcgtaacg
    51901 atcgtatctt ttttcaatcg atttttttct gccaagtatt tagcacagat aaacatgatt
    51961 ttatcaccgt cgacaatgtt tccaagttca tccaccgcaa taatacgatc gccatcgcca
    52021 tcgaaagcta gtccagcatc tgctcctttt tcaacgacca tctcagctaa acgttctgga
    52081 tgggtagagc ctacgccgtc attgatattt aacccgtctg gatttgtgcc cattgtataa
    52141 aaatctgttt caagatcggc aaacaaacga ttaacacttg ttgaagttgc tccgtttgct
    52201 gcatcgacac aaatggtcaa accggaaagg tcaccgttga tcgtttgtac aaggaattga
    52261 gaatatttca ataagccttc tggaaattct tctacagttc ccaatccttc agctgatgga
    52321 cgaggcaatt catccacttc agcatctaat aatgcttcaa tttctagctc ttggtcatct
    52381 actagtttaa aaccatctgc accgaaaaat ttgattccat tatcttgtgc tggattatga
    52441 gaagcagaaa tcatgacccc tgcgcttgct ttttgcaaac gtgtcagata agcaacacct
    52501 ggtgtagaga tcacacctag ctggaacact tcgataccca ccgataaaag tcctgaaatc
    52561 agcgcttctt ctaacatttg acctgagatt cttgtatccc gccctactaa cacgcgtggg
    52621 cgttttcctt cttcttcatg ctggcttagg acataacctc cgcaccgtcc cagcttaaat
    52681 gcaagttctg gcgtaagttc cttgttggct tcaccgcgta cgccgtctgt tccaaaatat
    52741 ttacccatag ttgtaaaatt actcctttaa cttaattttc tgaggaattt gttctgctca
    52801 tttgtgttga ttcgcttgtt tctgatgttt cttctgtact tgtggtcgag ctgcttgtcc
    52861 cagattccga actggaagaa atggttgttg tcgatggttc cgttcctgaa tcagcagatg
    52921 aaatacttct actcgtactt gtattttctt gtgtccgact gctcgtcgaa gcagagctac
    52981 tgcttgacgt ttgcaacacg ggtgtaatct ccacactaac tgattcgggc ttgatactta
    53041 acccctcttc tactggtatt tcaattttac gagttgttcg atgattgata cctgagacat
    53101 tgatcggtac agcaatactg tcaccgatcg atgcaagcaa atcgctagag ccgcttactt
    53161 ctgcttccag ttttgaaaaa ctgaaatcat agctttctac cccggcagct ggtgttcctt
    53221 gctgtatccc ataaagtcgt accgacttgc ttggcttgat cgaatcagct ctcactgtta
    53281 cttgcactgg atcagagacg atacttaata cttcccctgc tgcattcagc gcctgtattg
    53341 ggacctcact attaatccca tcatctgtga ccatcgaagc gtccacatca gctactactc
    53401 gtttgatttc tgccaatgtt ttgtcacctg tagtgataga gactttttta ggactaacgg
    53461 aagtatcatc aagttcatat ccagatggtg tgaacgttga aggaatcaac gtttccacat
    53521 cgaaatcttt tattaccttt ttctcaaccg ttactgtaat agtcgatggt tcgatctttg
    53581 ctgtgacagc actgctcaaa ttttgaattt ttagcggaac ttcatgtgtt ccttcgccca
    53641 gcttagtgat atctgctgtg acgcggaaca tccgtgtatc ttcgtttgcc tctgcattca
    53701 actgaactcg gtttgcactg gaaagtttga cagtcactgc attttcatat ccatggatat
    53761 agtagcgctt tgaatcataa gaaatcttga tcggcacccc cgtaactgtt tgattatacg
    53821 cttcatttcc agaaagcgta tttggaacgg actggccatt tgcattgaag tacagcgcaa
    53881 tactgaacag caatgcaata aaactgtaaa gaatgcttgt ccgtttctct tttgagatca
    53941 tttgcgatcc cctcccttag tcataccgtc taggaaagat tgaatcacgt ttttcttgtt
    54001 ttttgttttc tcttcagcaa ttagttctct acgcaagatt gccaaatatt cttgctggga
    54061 taagccagcc atcagttcat tatctaacgt gatgctgacg tccccggttt cttcagagac
    54121 aacgatggta atcgcgtcac tgacttcgct tataccgaca gctgcccggt gtcgagtccc
    54181 gaattcttta gggatcaaca tactttctga tagaggaaga taggcgcttg ctactgcgat
    54241 ctttccttct tttacaataa ctgcaccgtc atgcagcggt gtattaggaa taaagatatt
    54301 gatcagcagc tccccagtga tatctgcatc taaagcaatc cctgtctcaa tatactcatc
    54361 taatccagta tgacgttcga tagtgatcaa cgcaccgatt tttcgcttgg acatatattg
    54421 gatggctttg tcaaaagaca agatcatctt ctcatcttcc tgctgttcgc tttttgattg
    54481 tttgaaaagc gaactgcgtc caagatgttc cagtcctctc cggatttccg gctgaaaaat
    54541 cacgatagca gctatcaccc cgtacgtaat gacttggttc atcagccaag acaaggtgtg
    54601 cagaccaatg atttccgcta cgattcggat aacaataaaa atagctacac ctttcagaag
    54661 ctgaatcgct tttgtgcctc gcagcagttg gatcagtttg tatacaagat accagacaac
    54721 cagaatatca agtatgttca ccaaaaaatt cagagagaac aagtcaccgg aaaacatttg
    54781 ctgccaatag tccaaattaa aaagttcccc gatttgaaat gacataaaaa gcctctcttc
    54841 tcttttgaga tttcctctct agtatatcat atgttttaat tttagaggct gatatgctcg
    54901 tatttttctt gagaaaaacg cataattttc aagaaaattg caagtatgcg ttttattttt
    54961 ctctgcaata aatattttta aacttcagag aattattcat tatatttttt ctgttatttt
    55021 tccttttttt cttttgtagc ttgttccatt tttttacgtc gttctgctct ggcagctctt
    55081 ctagcagctc ttctttcttc tgcttcaagg tccgtcgttt tttcttttct agtcttaacg
    55141 ggtacatcga tagattcctc tggttcaagt cgtttcttga ttttttctaa ttctcccgtc
    55201 agtgcagcat agttgtaaaa acgtgtttta gcgtcgtgga cagtcttttc aaataaagct
    55261 tttgcaagat ttggatttgc gttatctagc gaggaaaagc gaacttgttt cctcatgaaa
    55321 tcaggcatag cagagaaatc aggttttttg tagtccaaag tcattgggtt cttgtttttt
    55381 tcgaccattg tagggttata tcgatataat gaccaataac cagaagaaac agcttctttt
    55441 gcttctttaa gcgattcttt cattccgcca agcaagccat gtgtgataca aggcgtatag
    55501 gcaataatca gagaaggacc aggatacttt tctgcttctt caaatgcctt gatcgtttgc
    55561 atttgattgg cacctgaagc aatttgcgcc acataaacat ttccatatgt catcgccatc
    55621 attcctagat ctttctttga agcatacttt ccactagccg aaaatttagc gattgccgaa
    55681 gctggtgttg cttttgaggt ttgaccgcca gtattggaat agacttcgtt atccaaaaca
    55741 aggatattca cgtctgctcc gctggcaagt acatgatcga tcccgccaaa accaatgtcg
    55801 taagcccatc cgtctccacc aatcatccat tggctgtttt ttacgaatag atcctttctc
    55861 ttgtatatat cctccagttc agggaaaatt gatcgttcgc tttcgatcgc agcttgcagt
    55921 tttgctgctc tttgctgcgt tccttcactc tcaaacatgt gtactaacca gtcttccatc
    55981 aattctcgta gttcttctga tacttttggc aatacttctt tgattgaact tgccaaagcc
    56041 tcgcgacgag tctgtgccgc tagaagcata ccataaccaa attcagcatt atcttccaat
    56101 aaagaatttg accatgcagg cccttgacca cattcattgg tcgtataagg agaagcagga
    56161 gaagcaccgc cccaaataga agaacagcct gtcgcattcg cgatcatcat tcgatcaccg
    56221 aacatttgtg tcaataattt tacataaggt gtttcgccac agcctgagca agcgcccgag
    56281 aattcaagaa gcggttgatt gaattgtgta gacaagacag tattctttcc tttgactgga
    56341 tttgcttttt gtcttaatgt cattgcaaaa gcccaattca cagcttgttc tttctgttct
    56401 tcataaggct tcatcactaa agctttttct tttgcgggac aagcctctac acacagacca
    56461 caacctgtac agtcttccac tgaaacttgg atacggtatt ttacgccgtc tgcgcctcgc
    56521 atttctctca cgataaatcc ttctggcgct tcttccattt cctcttcatc tgctaaaaat
    56581 ggacggatgg cagcatgagg acaaacaaag gcacattcat tacacatcgt acagcgatca
    56641 ctaatccact caggtacttc taaagcgacg ccccgctttt ccatagaagc cgttcctaaa
    56701 ggcatttcgc cagttgtcat tccatttgct acaagtgtac caacagagag ttgatttcct
    56761 tcctgtgcat tcactggttc aagtatttct tgtatataac cagtgttcct tttattgatt
    56821 ttttcaggca tctccaacgt tctccaatgg tctgggatcg cgacttgatg aagcagttcg
    56881 gtcgtccgat cgattgcttg tatgtttttt tctacgatag ccattgattt atgtccatag
    56941 cttttcaggg cttcttcttt tagcagcgga agcacctcat caaaaggaat gatgtcagct
    57001 aatttaaaaa aagccgtttc cattgccgta ttgatccggc gtcccaaacc gacttcttgg
    57061 gccaatctca tggcattgat cgtatagaat ctaatgttat tttcagccaa gtaacgtttt
    57121 aattttaagg gcagatgcgt ttctaattgt tcgtcgctcc aaagagtatt caataaaaaa
    57181 atgccacctg gcttcaatcc tttgaccaag tcatagctat gcaggtaagc tggtgtgtga
    57241 caggcaacaa ggtccgcatg ctcgacaaga tacgctgaac gtatcggcgt atctccaaaa
    57301 cgaagatgag agacagtcag accgccagat tttttcgaat cataataaaa atagccttgc
    57361 gcatatttat cggtgtgatc tccaataatc ttgatagctg atttatttgc tccaactgtt
    57421 ccgtcagatc caaatcccca aaacttggcc tggaatgttt gcggttctgt caaatccaga
    57481 ctttcctttg gttccaaaga ttgataagtc acatcatcca cgatccctat ggtaaaccgt
    57541 tttttgcgta tgcttgggtc ttttttcaat tcatcaaaaa ctgctgagat ttggtcggga
    57601 gtcacatctt tagaccccaa tccgtagcga ccaccaataa cagccgggcg aagttcagaa
    57661 tcatataacg cactttgaac gtctaataag agaggttctc cacctgcccc tggttcttta
    57721 cttcgatcaa gaacagcaat tgtttttact gtatgaggca acttttgcac gaatgtttcc
    57781 actggaaaag gacgataaag atggatattc aaaaaacctg ttttccgtcc gtttgcattc
    57841 aagtaatcaa tcgtttgttc aatcgtctgt gcaactgatc ccatagaaac aatgacttct
    57901 tccgcatcgt ctgctccgta ataagtcacc aagtcataag aagttcctcg taatcggttg
    57961 atctctttca tatatttttg aacgatttca ggtaatttat cataataggg gttgatcgtt
    58021 tcccgttgtt gaaaatggat gtctggattc tggttcgtac cactaacagt cggatgattg
    58081 ggattcatcc ctcgtttacg aaatgaagct aatttctcct gattgatcag aggagctaat
    58141 tcttcatatt ccaaaacttc gattttttga atctcgtgac tcgtacggaa tccatcaaag
    58201 aaattcaaaa aagggacttc tccttcaata gaagcaagat gggcaacagg tgctaaatcc
    58261 atcacttctt gtacactgct ttcagctaac atagcaaatc ctgtttgtct tgcagccatc
    58321 acgtctccgt gatctccgaa aatattcaac gcatttgtcg tcactgcccg actagcaacg
    58381 tggaaaacac ttggcagtag ttccccagca atcttgtaca tattcggtat catcaaaagc
    58441 agcccttgtg aagcggtata tgtagttgtc agtgcaccag ttttcaatga tccatggacc
    58501 acaccagctg ctcctgcttc ggattgcatt tctactactt ttactggttg accgaatacg
    58561 ttttcttttc ctgctgctga ccattgatct actaattcag ccatggtcga actgggtgtt
    58621 attggatata ttgcagcaat ctcggtaaat gcataagaaa tatatgcggc tgccgtattg
    58681 ccatccattg ttttcatttt tcgcatgatt catcatcctt gttcatattc tctcttatta
    58741 tctgttccat tataacgtaa aacatgccat ttttttcttt tatttataaa aatattttac
    58801 atgtatctat ttgaattggt catctaattt ttagacaaac agtctaaatc gttgcctttt
    58861 tttgtcagac atgtataaac tataagcgaa agaggtgttt taacatggat atatcagtga
    58921 ttgatgcaac gaaaacaaat acacaaaaag gaatccttta tggttcatcg aatgctccta
    58981 aaaaaatggt cgaattcatc aacttagctt gtccgtattg ccgacaatgg tttgaagaat
    59041 cttatgagct attagaagaa gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt
    59101 tcgataaaga aaaagaaagc ttacagcgag ggaacgtgat gcaccggtat ctcacaatca
    59161 gcgatggcca aaaagcgatc aaagagatca aacaaatatt cgatactcaa gacgagtgga
    59221 aacatctttc tctccaagaa gtagcggatt ttgcagtaga taaactaaag ctgacagaac
    59281 aaaaagacga acagctgagc caagctgtca tcaacgaagc tgagcaagca catattcgat
    59341 ttgttccaac tgtcatcttg ggtaaagaaa tctttgatga atctatttca atagaggaat
    59401 taaaagaatt gatccaaaca aaataaaaaa ataaggagcg tgggacaaaa gtaaagaaca
    59461 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcttatt
    59521 tcttggagtt aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc
    59581 ttgcatctgc cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat
    59641 aatttattca tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt
    59701 tctcaatttc ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca
    59761 ctttttaagt tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt
    59821 tagttgatta gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt
    59881 agcttatacc tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg
    59941 tatttgccac tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg
    60001 ctctcttttt tattcagatt catttttgct gatcacagcc tagtatttca atggttttga
    60061 acactaaacc aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt
    60121 tcgattattc aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc
    60181 caaacctatc gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt
    60241 tattgcgtgg atattctatt gttacgccat taaatggata ccaattagga ttaggctctc
    60301 tttttttctg tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc
    60361 gaccatcctc taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta
    60421 gacgccacgt gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg
    60481 caacaattgt atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat
    60541 caaactcccc tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta
    60601 aaaccgcatt cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt
    60661 ttctatttga taaatttctc attattcgca catcacctct acattctcac atattagttc
    60721 gtaatactcc tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct
    60781 actactcgta taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt
    60841 ctatttatcg taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc
    60901 cagttgatta aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt
    60961 acagttctta tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc
    61021 tcgagatttc taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata
    61081 taatattgcg cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct
    61141 tctctaccac tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg
    61201 aactcacacc cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct
    61261 agttcgtatt caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta
    61321 ggtgagatta atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct
    61381 atgatcacaa ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg
    61441 tattccattt tattccccct attactattc atatccgcct atctatatcg atcttccaat
    61501 gcagagataa ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt
    61561 ctccactaag gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac
    61621 tcctccaaaa acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt
    61681 atatgtgttt gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat
    61741 cgcattcgta tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat
    61801 cattgtgatt tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta
    61861 tagaaatcgc ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg
    61921 tctccatttt tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca
    61981 caacacgtta ccttttaaat cattatactg tatctcatac attattatca ataaactatt
    62041 ataaaaatgc aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa
    62101 tcataccctg tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg
    62161 caagacagac actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt
    62221 acccactttt gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac
    62281 cccaagaaat taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca
    62341 acatttaaat tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata
    62401 tcaagatgtc aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta
    62461 ttaagactaa tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat
    62521 gatcctgcaa gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc
    62581 tttttcccat taggatcttt tattttatga attttctgca ggtaatctct cctaatggca
    62641 gctattttta cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt
    62701 atgttgtgtt tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa
    62761 agttgaatga gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca
    62821 tatcctcccg caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa
    62881 gagaaaagag tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag
    62941 ataacaactc caaacattga atttttatac aatgaccatt tatttatagg cttttctttc
    63001 attttacttc cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc
    63061 tctttgaaca tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt
    63121 tataggcgag ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact
    63181 gaaattaaag cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac
    63241 atgtctttac ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg
    63301 taatactttt tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata
    63361 atatctaatc taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa
    63421 ataaatagaa actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgtat
//
LOCUS       DAJSBC010000008        62714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSBC010000008 DAJSBC010000000
VERSION     DAJSBC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.008"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT65_000502"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT65_000502"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR2328156.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT65_000503"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT65_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR2328157.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT65_000504"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT65_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR2328158.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT65_000505"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT65_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR2328159.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT65_000506"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT65_000506"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR2328160.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT65_000507"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT65_000507"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR2328161.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ONT65_000508"
     CDS             complement(8053..8523)
                     /locus_tag="ONT65_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR2328162.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ONT65_000509"
     CDS             complement(8569..8994)
                     /locus_tag="ONT65_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR2328163.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ONT65_000510"
     CDS             complement(9061..9330)
                     /locus_tag="ONT65_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR2328164.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ONT65_000511"
     CDS             complement(9341..10936)
                     /locus_tag="ONT65_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2328165.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT65_000512"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT65_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR2328166.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="ONT65_000513"
     CDS             complement(14489..15049)
                     /locus_tag="ONT65_000513"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR2328167.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ONT65_000514"
     CDS             15402..16376
                     /locus_tag="ONT65_000514"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2328168.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ONT65_000515"
     CDS             16546..17946
                     /locus_tag="ONT65_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR2328169.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ONT65_000516"
     CDS             complement(18024..18746)
                     /locus_tag="ONT65_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2328170.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ONT65_000517"
     CDS             complement(18825..19331)
                     /locus_tag="ONT65_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2328171.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ONT65_000518"
     CDS             19454..21253
                     /locus_tag="ONT65_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR2328172.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ONT65_000519"
     CDS             complement(21321..22895)
                     /locus_tag="ONT65_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2328173.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT65_000520"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT65_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR2328174.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT65_000521"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT65_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR2328175.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ONT65_000522"
     CDS             complement(24672..25310)
                     /locus_tag="ONT65_000522"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR2328176.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="ONT65_000523"
     CDS             complement(25311..25961)
                     /locus_tag="ONT65_000523"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR2328177.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT65_000524"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT65_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR2328178.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT65_000525"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT65_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR2328179.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ONT65_000526"
     CDS             complement(29104..29844)
                     /locus_tag="ONT65_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR2328180.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT65_000527"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT65_000527"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR2328181.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="ONT65_000528"
     CDS             complement(31215..32153)
                     /locus_tag="ONT65_000528"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR2328182.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT65_000529"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT65_000529"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2328183.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT65_000530"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT65_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR2328184.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="ONT65_000531"
     CDS             complement(35313..35618)
                     /locus_tag="ONT65_000531"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2328185.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT65_000532"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT65_000532"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR2328186.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ONT65_000533"
     CDS             complement(36724..37008)
                     /locus_tag="ONT65_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328187.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ONT65_000534"
     CDS             complement(37026..37916)
                     /locus_tag="ONT65_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR2328188.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ONT65_000535"
     CDS             complement(37936..38154)
                     /locus_tag="ONT65_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR2328189.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ONT65_000536"
     CDS             complement(38124..39824)
                     /locus_tag="ONT65_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR2328190.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ONT65_000537"
     CDS             40091..40864
                     /locus_tag="ONT65_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328191.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT65_000538"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT65_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR2328192.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ONT65_000539"
     CDS             complement(41688..42659)
                     /locus_tag="ONT65_000539"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2328193.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT65_000540"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT65_000540"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR2328194.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT65_000541"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT65_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR2328195.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ONT65_000542"
     CDS             complement(45614..46438)
                     /locus_tag="ONT65_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2328196.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ONT65_000543"
     CDS             complement(46411..47091)
                     /locus_tag="ONT65_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2328197.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ONT65_000544"
     CDS             complement(47114..48052)
                     /locus_tag="ONT65_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR2328198.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="ONT65_000545"
     CDS             complement(48297..49160)
                     /locus_tag="ONT65_000545"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR2328199.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT65_000546"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT65_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2328200.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ONT65_000547"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ONT65_000547"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR2328201.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT65_000548"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT65_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR2328202.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT65_000549"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT65_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR2328203.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ONT65_000550"
     CDS             complement(56026..57267)
                     /locus_tag="ONT65_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR2328204.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT65_000551"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT65_000551"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR2328205.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ONT65_000552"
     CDS             complement(58294..59217)
                     /locus_tag="ONT65_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328206.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ONT65_000553"
     CDS             complement(59956..61242)
                     /locus_tag="ONT65_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2328207.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ONT65_000554"
     CDS             complement(61235..62503)
                     /locus_tag="ONT65_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2328208.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
BASE COUNT        17307 a        13635 c        11225 g        20547 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttt
//
LOCUS       DAJSBC010000009        61364 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSBC010000009 DAJSBC010000000
VERSION     DAJSBC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.009"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..561)
                     /locus_tag="ONT65_000555"
     CDS             complement(<1..561)
                     /locus_tag="ONT65_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR2328209.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="ONT65_000556"
     CDS             complement(748..1398)
                     /locus_tag="ONT65_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328210.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ONT65_000557"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ONT65_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR2328211.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="ONT65_000558"
     CDS             complement(2910..3908)
                     /locus_tag="ONT65_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR2328212.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ONT65_000559"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ONT65_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR2328213.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="ONT65_000560"
     CDS             complement(4924..5655)
                     /locus_tag="ONT65_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR2328214.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="ONT65_000561"
     CDS             complement(5659..6924)
                     /locus_tag="ONT65_000561"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR2328215.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ONT65_000562"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ONT65_000562"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR2328216.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ONT65_000563"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ONT65_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR2328217.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="ONT65_000564"
     CDS             12266..13768
                     /locus_tag="ONT65_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR2328218.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="ONT65_000565"
     CDS             13770..14507
                     /locus_tag="ONT65_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2328219.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="ONT65_000566"
     CDS             complement(14552..14725)
                     /locus_tag="ONT65_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328220.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="ONT65_000567"
     CDS             14701..15366
                     /locus_tag="ONT65_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328221.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ONT65_000568"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ONT65_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR2328222.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ONT65_000569"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ONT65_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR2328223.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ONT65_000570"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ONT65_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR2328224.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ONT65_000571"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ONT65_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR2328225.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20579..21745)
                     /locus_tag="ONT65_000572"
     CDS             complement(20579..21745)
                     /locus_tag="ONT65_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR2328226.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21761..21988)
                     /locus_tag="ONT65_000573"
     CDS             complement(21761..21988)
                     /locus_tag="ONT65_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR2328227.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22472..23062
                     /locus_tag="ONT65_000574"
     CDS             22472..23062
                     /locus_tag="ONT65_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR2328228.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23252..23965
                     /locus_tag="ONT65_000575"
     CDS             23252..23965
                     /locus_tag="ONT65_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328229.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24080..25090)
                     /locus_tag="ONT65_000576"
     CDS             complement(24080..25090)
                     /locus_tag="ONT65_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR2328230.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25127..25609)
                     /locus_tag="ONT65_000577"
     CDS             complement(25127..25609)
                     /locus_tag="ONT65_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR2328231.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25759..26127
                     /locus_tag="ONT65_000578"
     CDS             25759..26127
                     /locus_tag="ONT65_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR2328232.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26592..27044
                     /locus_tag="ONT65_000579"
     CDS             26592..27044
                     /locus_tag="ONT65_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328233.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27168..28019)
                     /locus_tag="ONT65_000580"
     CDS             complement(27168..28019)
                     /locus_tag="ONT65_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR2328234.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28032..28817)
                     /locus_tag="ONT65_000581"
     CDS             complement(28032..28817)
                     /locus_tag="ONT65_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2328235.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="ONT65_000582"
     CDS             complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="ONT65_000582"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR2328236.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30114..31037)
                     /gene="truB"
                     /locus_tag="ONT65_000583"
     CDS             complement(30114..31037)
                     /gene="truB"
                     /locus_tag="ONT65_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR2328237.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31405..33767)
                     /locus_tag="ONT65_000584"
                     /pseudo
     CDS             complement(31405..33767)
                     /locus_tag="ONT65_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34215..34388
                     /locus_tag="ONT65_000585"
     CDS             34215..34388
                     /locus_tag="ONT65_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2328238.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="ONT65_000586"
     CDS             complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="ONT65_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR2328239.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34919..37225)
                     /gene="infB"
                     /locus_tag="ONT65_000587"
     CDS             complement(34919..37225)
                     /gene="infB"
                     /locus_tag="ONT65_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR2328240.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37238..37549)
                     /locus_tag="ONT65_000588"
     CDS             complement(37238..37549)
                     /locus_tag="ONT65_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR2328241.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37546..37839)
                     /locus_tag="ONT65_000589"
     CDS             complement(37546..37839)
                     /locus_tag="ONT65_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR2328242.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="ONT65_000590"
     CDS             complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="ONT65_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR2328243.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="ONT65_000591"
     CDS             complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="ONT65_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR2328244.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39677..44035)
                     /locus_tag="ONT65_000592"
     CDS             complement(39677..44035)
                     /locus_tag="ONT65_000592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR2328245.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44247..45956)
                     /locus_tag="ONT65_000593"
     CDS             complement(44247..45956)
                     /locus_tag="ONT65_000593"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR2328246.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="ONT65_000594"
     CDS             complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="ONT65_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR2328247.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47453..48253)
                     /locus_tag="ONT65_000595"
     CDS             complement(47453..48253)
                     /locus_tag="ONT65_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR2328248.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48250..49062)
                     /locus_tag="ONT65_000596"
     CDS             complement(48250..49062)
                     /locus_tag="ONT65_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR2328249.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49257..49814)
                     /gene="frr"
                     /locus_tag="ONT65_000597"
     CDS             complement(49257..49814)
                     /gene="frr"
                     /locus_tag="ONT65_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR2328250.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49817..50539)
                     /locus_tag="ONT65_000598"
     CDS             complement(49817..50539)
                     /locus_tag="ONT65_000598"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR2328251.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50675..51556)
                     /locus_tag="ONT65_000599"
     CDS             complement(50675..51556)
                     /locus_tag="ONT65_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR2328252.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="ONT65_000600"
     CDS             complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="ONT65_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR2328253.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52796..53275
                     /locus_tag="ONT65_000601"
     CDS             52796..53275
                     /locus_tag="ONT65_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR2328254.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53492..54583
                     /gene="phnW"
                     /locus_tag="ONT65_000602"
     CDS             53492..54583
                     /gene="phnW"
                     /locus_tag="ONT65_000602"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR2328255.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54576..54704
                     /locus_tag="ONT65_000603"
     CDS             54576..54704
                     /locus_tag="ONT65_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2328256.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54708..55253
                     /locus_tag="ONT65_000604"
     CDS             54708..55253
                     /locus_tag="ONT65_000604"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2328257.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55707..57398)
                     /locus_tag="ONT65_000605"
     CDS             complement(55707..57398)
                     /locus_tag="ONT65_000605"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR2328258.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="ONT65_000606"
     CDS             complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="ONT65_000606"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR2328259.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58880..59899)
                     /gene="argF"
                     /locus_tag="ONT65_000607"
     CDS             complement(58880..59899)
                     /gene="argF"
                     /locus_tag="ONT65_000607"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR2328260.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="ONT65_000608"
     CDS             complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="ONT65_000608"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR2328261.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17058 a        13102 c        10623 g        20581 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt
    20521 tattgtattt ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact
    20581 atttttcaaa caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca
    20641 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    20701 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    20761 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    20821 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    20881 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    20941 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    21001 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    21061 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    21121 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    21181 ccaaagcttt ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa
    21241 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca
    21301 catcttctgc ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca
    21361 ctcccatgga taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca
    21421 aatcacctgg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    21481 tagcacctga taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc
    21541 ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat
    21601 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat
    21661 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    21721 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    21781 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    21841 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    21901 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    21961 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    22021 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    22081 aatccaagaa tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata
    22141 tatctctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    22201 ttactttttg ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt
    22261 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    22321 tttatttagt tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa
    22381 ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc
    22441 cttgttatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    22501 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg
    22561 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    22621 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    22681 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    22741 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    22801 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    22861 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    22921 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    22981 agaaataaga gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa
    23041 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg
    23101 acaagtaatt ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata
    23161 cactctattt atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata
    23221 ctggtattaa aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt
    23281 ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc
    23341 gaccacagac aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa
    23401 atcagcttct actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac
    23461 ttcttctaca tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac
    23521 ctcctcacag tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga
    23581 gtacgcacgt gtttggctag ctattagagg taatactttt agagatacat cgggtgattt
    23641 cgaattacat gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag
    23701 tgcagtctat ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt
    23761 agtgtattca agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca
    23821 aatgcctgca gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat
    23881 tgataatgca tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat
    23941 cgatgtgaca gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag
    24001 cattttgtca gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc
    24061 caaggcagga aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta
    24121 ccgctacttc tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat
    24181 caatatcata caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca
    24241 ttttataatc taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat
    24301 caaaccagcc tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct
    24361 cacagttttc tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt
    24421 ggacatctcc ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata
    24481 atcgtccctt catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga
    24541 gatggaaaac agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag
    24601 attgctgtag aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg
    24661 tttcatccaa gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc
    24721 ctgtttttaa tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt
    24781 ttggttgtag agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc
    24841 aagtcggaaa gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc
    24901 gttttcttgc tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta
    24961 cttggaaaga tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct
    25021 ctgatacgcc tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat
    25081 aacttatcat gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt
    25141 cgtttgccgg catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa
    25201 ccgtttggat gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg
    25261 ataagagctc ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag
    25321 cgccctgctt ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt
    25381 taccgacgat cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata
    25441 cgtagttatt atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc
    25501 cttgttcttt ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca
    25561 actcaaaata tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc
    25621 ttttctattt tattatttga gacatcttat catataaata tagcggttac aacattactt
    25681 gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact
    25741 taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa
    25801 gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta
    25861 gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca
    25921 tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt
    25981 gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg
    26041 aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat tgaagataaa
    26101 aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg
    26161 aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt
    26221 aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa
    26281 aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt cagcaatttt
    26341 cctgcaaaac ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac
    26401 tgcccattat tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt
    26461 tagagaaata aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg
    26521 ttctttctac atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag
    26581 gagggtgaac attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta
    26641 ctacacgttc tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg
    26701 ggcaatggaa attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg
    26761 aagcagcaaa agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc
    26821 ttcaatatcg tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata
    26881 acgatgttta cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc
    26941 atgcggagat actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca
    27001 atgaaaaatt attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct
    27061 agaaattcca gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg
    27121 ttcaaacaaa tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc
    27181 gtgaccatgc ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt
    27241 ggtgtcttat tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc
    27301 tgtgtaatga tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat
    27361 gctgacacac taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat
    27421 gcggttggtt tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc
    27481 ggaaagaaaa tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa
    27541 taaccaatac ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca
    27601 ggatcatcac tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct
    27661 ccaatgattt tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag
    27721 gttccgacta actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg
    27781 aaagatgtat actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc
    27841 aaaaatgtag tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca
    27901 agcaccgctc caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc
    27961 aaaatcagtg gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt
    28021 tttattcacc cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct
    28081 gcaaatagtg taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc
    28141 gccgctattt cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct
    28201 ttttgttcag gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct
    28261 atactattga ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat
    28321 tttaccccgc cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga
    28381 acagaagaca tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc
    28441 gctttcgttc cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca
    28501 aggctaagtt catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc
    28561 agtgtcccaa aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg
    28621 acatctgctt gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt
    28681 tctgctcctt ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat
    28741 cgttgagcaa tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt
    28801 tctaattcct tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata
    28861 acttttattt ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata
    28921 cgctctagca atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt
    28981 tgtgacagaa gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga
    29041 aaaaccattt atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg
    29101 gcttatttat cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc
    29161 ataatgtttt attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt
    29221 tcaatcaatt cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt
    29281 accgcgacat gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc
    29341 agctcccgtc cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat
    29401 gtttccccaa tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc
    29461 tttactttga catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca
    29521 actacccctt ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg
    29581 ctgtctagta atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta
    29641 gaaactgtga cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca
    29701 atgtcttttg gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct
    29761 acgatgtatt gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca
    29821 acttcatata aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta
    29881 gtcaaatatt tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg
    29941 aaagtcatca atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg
    30001 actttctggt gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct
    30061 tctggaattt gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc
    30121 tcatttctca atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa
    30181 atactgacaa cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc
    30241 gcaaatacag tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta
    30301 atatctactc gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt
    30361 gtcccatttt tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata
    30421 ccggcacttg ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct
    30481 gtatctacag ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa
    30541 gaaatggttc ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc
    30601 tgagcctttc taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc
    30661 ttaccgttga cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat
    30721 gaagccatca cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct
    30781 acccgttctc cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca
    30841 tagattttcc cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg
    30901 ggcaacacac cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt
    30961 aagatttttc ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa
    31021 ggtaataacc cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa
    31081 cacattgcat caattactga cgaatttgac atcataatga acggttttta tttttcggca
    31141 ataaaaacgt aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag
    31201 gagggaatag aatggaagat tttttctttg caccatttga ttggatttat gggtggctta
    31261 caggtaaata gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca
    31321 gtcttttttc cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta
    31381 ttttgcggga tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac
    31441 ttctggcaga tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc
    31501 catttttttc gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc
    31561 caaatgataa cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc
    31621 attttctttg tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg
    31681 gaccaatgtt tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg
    31741 atcaaattct tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa
    31801 atctacgaca ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac
    31861 agctgctaat gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc
    31921 ctcctctttt gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc
    31981 tgcttcttct gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat
    32041 tttttctgac gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg
    32101 cagatattca cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc
    32161 ttggtggcta tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag
    32221 cgaaggataa tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg
    32281 agtcaacata gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt
    32341 ccagtcagaa cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg
    32401 atgacgcgac cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat
    32461 tggcgatttg tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca
    32521 aatatacgga aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg
    32581 gtcatatctt ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa
    32641 tctggcataa taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt
    32701 ctttggtcac cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca
    32761 aataattttt ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata
    32821 tgttcttgat ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc
    32881 ccgtcccatt cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat
    32941 tttggcatca cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt
    33001 ttctcgattg cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt
    33061 ccttcaataa agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct
    33121 gatttacgtg ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt
    33181 aaaacagctc catctatcac tggattgata aagacatttg agaaccagct tccagctaaa
    33241 ggaccggttt ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct
    33301 aatacagctc cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa
    33361 cctggtgttt ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat
    33421 aatttttgca ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca
    33481 tctaaataag cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac
    33541 atgttcaaat catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca
    33601 ggaatcgttc cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt
    33661 ctcagtaagg ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc
    33721 caccaagccg tcattttatc aaagtattct ttactagaat aatccataca taacactcct
    33781 tttcattatt actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct
    33841 gttcatttgt ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt
    33901 ttcctttcgc tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt
    33961 tccgcagttc atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa
    34021 cacattgctg gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat
    34081 gtaaaagtct ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt
    34141 tctacaggga ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata
    34201 tcaataactt caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt
    34261 atggtgcagg ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag
    34321 attacaatct agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat
    34381 actactagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg
    34441 acacgccgca agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa
    34501 aatcccgcaa cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag
    34561 tcgcgaagca attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac
    34621 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
    34681 gctttattca atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg
    34741 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
    34801 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
    34861 tgaccgactc ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
    34921 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
    34981 tgaatttttc aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt
    35041 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
    35101 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
    35161 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
    35221 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
    35281 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
    35341 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
    35401 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
    35461 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
    35521 cttctttcaa ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg
    35581 tggcagcacg gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg
    35641 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
    35701 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
    35761 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc
    35821 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
    35881 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
    35941 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga
    36001 tcgtttctcc accccatgct tcaggaatta actcatactc acttaattct tgcataacat
    36061 gttgagggtt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
    36121 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
    36181 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
    36241 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
    36301 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat
    36361 ccaataaagt tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg
    36421 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
    36481 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
    36541 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
    36601 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
    36661 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
    36721 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
    36781 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
    36841 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
    36901 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
    36961 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
    37021 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
    37081 cttgttgcaa tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga
    37141 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
    37201 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
    37261 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
    37321 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
    37381 cttttatctt ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt
    37441 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
    37501 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
    37561 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
    37621 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
    37681 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
    37741 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
    37801 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
    37861 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
    37921 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
    37981 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
    38041 agtcaattta gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg
    38101 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
    38161 gacttgtgca ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac
    38221 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
    38281 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
    38341 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
    38401 tacttcttgc tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg
    38461 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
    38521 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
    38581 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
    38641 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
    38701 ttgtttagcc gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat
    38761 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
    38821 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
    38881 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
    38941 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
    39001 taaagcgtct aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt
    39061 aaaattggat agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt
    39121 ctcgtgtttt tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc
    39181 cttcatattg tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa
    39241 gtgccttttc atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct
    39301 ccaggaaata agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt
    39361 tctcactgac aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc
    39421 gtaagaacca gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt
    39481 catctaaaat cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg
    39541 cctccttaaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
    39601 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
    39661 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
    39721 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
    39781 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg
    39841 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
    39901 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
    39961 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
    40021 ctaatccttt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
    40081 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
    40141 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
    40201 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
    40261 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
    40321 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
    40381 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
    40441 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
    40501 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag
    40561 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
    40621 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
    40681 gtggatcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
    40741 gaatatctaa tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc
    40801 attccgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
    40861 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
    40921 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
    40981 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
    41041 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
    41101 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
    41161 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
    41221 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
    41281 gacaataata atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa
    41341 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
    41401 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
    41461 ctttttttaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
    41521 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
    41581 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
    41641 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
    41701 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
    41761 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
    41821 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
    41881 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
    41941 taacttcaat ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca
    42001 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
    42061 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
    42121 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
    42181 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
    42241 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
    42301 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag
    42361 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
    42421 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
    42481 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
    42541 acatccgcaa tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag
    42601 tcagatctac cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt
    42661 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
    42721 cagacaatcc tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat
    42781 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
    42841 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
    42901 cgtggtctgt aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag
    42961 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
    43021 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
    43081 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
    43141 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
    43201 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
    43261 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
    43321 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
    43381 tgtggatggg tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt
    43441 ggacaactag actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt
    43501 cttcaaaaag tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa
    43561 attttgggaa tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca
    43621 aataagaaat agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag
    43681 gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag
    43741 cttgggccca gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat
    43801 tcagtttgac ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat
    43861 atagcataat aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac
    43921 gatggaccac tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt
    43981 cctctagttg gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata
    44041 tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt
    44101 accttttagt atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa
    44161 atcgcagttc caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat
    44221 ttttattttc tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta
    44281 ttcaccaatt cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct
    44341 acgattccat ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca
    44401 gaatccgcga atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca
    44461 gcttttgtca tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga
    44521 ttcatttgga cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt
    44581 tcatctgaat gttgttctac gattgctgat aataaacgac ttacaccgat accataacag
    44641 cccataatca caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca
    44701 ctgtaacgag tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga
    44761 acaccattgt tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa
    44821 agtacagtga aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct
    44881 ccagtaatgg cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct
    44941 gaaacaccga ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct
    45001 tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact
    45061 tcatggtctc cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac
    45121 ttgatgattt tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg
    45181 ttaggtgtag ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag
    45241 tagcttgtag ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta
    45301 tcctccccga tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca
    45361 ccgtctccga taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct
    45421 ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca
    45481 tctttcatga taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga
    45541 tatttaggtt ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta
    45601 cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg
    45661 cggtctttga aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa
    45721 agttctgctg gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct
    45781 tcccgcataa tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa
    45841 atacccgctg atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact
    45901 tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta
    45961 tatactcctt tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt
    46021 tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa
    46081 aaatccaaag ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg
    46141 aatcccttca aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag
    46201 attgacgatt ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc
    46261 ttgatttgct gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa
    46321 actgaaacct gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc
    46381 ttgacgcgtc cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc
    46441 aagttggcca actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg
    46501 cacctgacct tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac
    46561 tgttgtcaga tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt
    46621 ttcttttaag cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt
    46681 gttcgtcact tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa
    46741 aataaagtta ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga
    46801 ttggaactgt acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt
    46861 cgcatcgtga tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt
    46921 gatagtcagc tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa
    46981 ttggattttc ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga
    47041 aagaggcatt cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata
    47101 accgccaata ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata
    47161 aattttcgga cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa
    47221 gaagaaatga ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag
    47281 aatcgttttc atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga
    47341 taaaacacta taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa
    47401 aataaaaacc tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa
    47461 tgccaaataa atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc
    47521 caccatggcc tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag
    47581 attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga
    47641 caaacatacc atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga
    47701 ctacaacagc actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat
    47761 caggacttaa cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg
    47821 cccaaacaat aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa
    47881 aattctggaa acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa
    47941 tcgtatacgt atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa
    48001 ataacataaa tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa
    48061 caagtaacac tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt
    48121 tcatccgaaa cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg
    48181 ctatgcctcc gatccatata atcggaataa acaagactag cgccacggct gctgtgacca
    48241 cacgttgttt catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg
    48301 acggtctcga ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg
    48361 ccaaagcgct tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact
    48421 aattcgctct tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa
    48481 gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc
    48541 tccttttgct acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc
    48601 ataattcaaa gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg
    48661 tcttacagca tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt
    48721 gacattttct ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat
    48781 gagaaaactt acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt
    48841 caacactttc actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc
    48901 ttctttatgg ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc
    48961 catgatgatg gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt
    49021 ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact
    49081 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
    49141 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
    49201 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
    49261 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
    49321 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
    49381 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
    49441 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
    49501 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
    49561 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
    49621 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
    49681 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
    49741 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
    49801 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
    49861 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
    49921 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
    49981 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
    50041 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
    50101 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
    50161 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
    50221 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
    50281 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
    50341 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
    50401 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
    50461 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
    50521 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
    50581 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
    50641 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
    50701 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
    50761 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
    50821 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
    50881 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
    50941 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
    51001 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
    51061 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
    51121 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
    51181 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
    51241 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
    51301 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
    51361 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
    51421 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
    51481 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
    51541 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
    51601 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
    51661 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
    51721 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
    51781 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
    51841 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
    51901 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
    51961 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
    52021 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
    52081 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
    52141 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
    52201 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
    52261 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
    52321 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
    52381 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
    52441 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
    52501 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
    52561 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
    52621 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
    52681 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
    52741 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
    52801 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
    52861 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
    52921 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
    52981 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
    53041 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
    53101 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
    53161 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
    53221 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
    53281 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
    53341 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
    53401 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
    53461 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
    53521 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
    53581 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
    53641 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
    53701 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
    53761 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
    53821 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
    53881 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
    53941 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
    54001 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
    54061 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
    54121 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
    54181 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
    54241 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
    54301 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
    54361 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
    54421 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
    54481 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
    54541 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
    54601 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
    54661 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
    54721 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
    54781 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
    54841 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
    54901 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
    54961 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
    55021 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
    55081 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
    55141 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
    55201 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
    55261 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
    55321 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
    55381 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
    55441 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
    55501 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
    55561 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
    55621 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
    55681 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
    55741 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
    55801 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
    55861 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
    55921 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
    55981 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
    56041 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
    56101 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
    56161 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
    56221 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
    56281 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
    56341 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
    56401 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
    56461 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
    56521 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
    56581 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
    56641 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
    56701 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
    56761 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
    56821 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
    56881 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
    56941 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
    57001 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
    57061 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
    57121 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
    57181 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
    57241 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
    57301 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
    57361 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
    57421 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
    57481 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
    57541 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
    57601 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
    57661 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
    57721 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
    57781 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
    57841 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
    57901 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
    57961 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
    58021 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
    58081 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
    58141 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
    58201 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
    58261 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
    58321 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
    58381 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
    58441 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
    58501 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
    58561 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
    58621 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
    58681 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
    58741 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
    58801 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
    58861 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
    58921 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
    58981 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
    59041 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    59101 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    59161 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    59221 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    59281 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    59341 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    59401 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    59461 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    59521 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    59581 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    59641 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    59701 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    59761 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    59821 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    59881 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    59941 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    60001 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    60061 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    60121 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    60181 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    60241 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    60301 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    60361 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    60421 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    60481 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    60541 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    60601 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    60661 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    60721 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    60781 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    60841 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    60901 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    60961 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    61021 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    61081 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    61141 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    61201 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    61261 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    61321 aatattcgat aattaaataa ttaaacgttt ttctattttt aagc
//
LOCUS       DAJSBC010000010        60388 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSBC010000010 DAJSBC010000000
VERSION     DAJSBC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.010"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(12..1535)
                     /locus_tag="ONT65_000609"
     CDS             complement(12..1535)
                     /locus_tag="ONT65_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2328262.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ONT65_000610"
     CDS             1787..2968
                     /locus_tag="ONT65_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2328263.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT65_000611"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT65_000611"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR2328264.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ONT65_000612"
     CDS             complement(5053..7764)
                     /locus_tag="ONT65_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328265.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ONT65_000613"
     CDS             complement(7774..8475)
                     /locus_tag="ONT65_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328266.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ONT65_000614"
     CDS             8661..9260
                     /locus_tag="ONT65_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2328267.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ONT65_000615"
     CDS             9341..10354
                     /locus_tag="ONT65_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2328268.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT65_000616"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT65_000616"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR2328269.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT65_000617"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT65_000617"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR2328270.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ONT65_000618"
     CDS             11802..13175
                     /locus_tag="ONT65_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2328271.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ONT65_000619"
     CDS             13532..15676
                     /locus_tag="ONT65_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2328272.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ONT65_000620"
     CDS             complement(15728..16768)
                     /locus_tag="ONT65_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2328273.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ONT65_000621"
     CDS             complement(17045..18847)
                     /locus_tag="ONT65_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328274.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ONT65_000622"
     CDS             complement(18861..19613)
                     /locus_tag="ONT65_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328275.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ONT65_000623"
     CDS             complement(19622..19984)
                     /locus_tag="ONT65_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2328276.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ONT65_000624"
     CDS             complement(20160..21032)
                     /locus_tag="ONT65_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2328277.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ONT65_000625"
     CDS             complement(21058..21570)
                     /locus_tag="ONT65_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR2328278.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ONT65_000626"
     CDS             complement(21708..22499)
                     /locus_tag="ONT65_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR2328279.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ONT65_000627"
     CDS             complement(22554..23162)
                     /locus_tag="ONT65_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR2328280.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ONT65_000628"
     CDS             complement(23155..23472)
                     /locus_tag="ONT65_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2328281.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ONT65_000629"
     CDS             complement(23628..24356)
                     /locus_tag="ONT65_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR2328282.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ONT65_000630"
     CDS             24515..25396
                     /locus_tag="ONT65_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2328283.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ONT65_000631"
     CDS             complement(25633..26676)
                     /locus_tag="ONT65_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2328284.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLYFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ONT65_000632"
     CDS             complement(26679..27347)
                     /locus_tag="ONT65_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2328285.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ONT65_000633"
     CDS             27489..29825
                     /locus_tag="ONT65_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328286.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ONT65_000634"
     CDS             complement(29940..30251)
                     /locus_tag="ONT65_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328287.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ONT65_000635"
     CDS             30432..31907
                     /locus_tag="ONT65_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR2328288.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ONT65_000636"
     CDS             31954..32538
                     /locus_tag="ONT65_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR2328289.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ONT65_000637"
     CDS             32540..32959
                     /locus_tag="ONT65_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR2328290.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ONT65_000638"
     CDS             complement(33240..33524)
                     /locus_tag="ONT65_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR2328291.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ONT65_000639"
     CDS             complement(33876..34586)
                     /locus_tag="ONT65_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR2328292.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ONT65_000640"
     CDS             complement(34583..36055)
                     /locus_tag="ONT65_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2328293.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ONT65_000641"
     CDS             36193..36666
                     /locus_tag="ONT65_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2328294.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ONT65_000642"
     CDS             complement(36819..37370)
                     /locus_tag="ONT65_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2328295.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ONT65_000643"
     CDS             37475..38005
                     /locus_tag="ONT65_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR2328296.1"
                     /translation="MKEFKELEDFIGTRFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ONT65_000644"
     CDS             complement(38488..38931)
                     /locus_tag="ONT65_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2328297.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ONT65_000645"
     CDS             complement(38947..40107)
                     /locus_tag="ONT65_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2328298.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ONT65_000646"
     CDS             40614..42314
                     /locus_tag="ONT65_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328299.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPSFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ONT65_000647"
     CDS             complement(42416..43249)
                     /locus_tag="ONT65_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR2328300.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ONT65_000648"
     CDS             complement(43553..45718)
                     /locus_tag="ONT65_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2328301.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ONT65_000649"
     CDS             45978..48272
                     /locus_tag="ONT65_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2328302.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT65_000650"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT65_000650"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2328303.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ONT65_000651"
     CDS             48923..49930
                     /locus_tag="ONT65_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2328304.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ONT65_000652"
     CDS             49952..50971
                     /locus_tag="ONT65_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2328305.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT65_000653"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT65_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2328306.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="ONT65_000654"
     CDS             complement(52729..54150)
                     /locus_tag="ONT65_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2328307.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT65_000655"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT65_000655"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2328308.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="ONT65_000656"
     CDS             complement(56561..57817)
                     /locus_tag="ONT65_000656"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR2328309.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ONT65_000657"
     CDS             58397..59698
                     /locus_tag="ONT65_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2328310.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ONT65_000658"
     CDS             complement(59767..60366)
                     /locus_tag="ONT65_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2328311.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18212 a        12362 c        10827 g        18987 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaataca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcgcttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatctttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaatttt
//
LOCUS       DAJSBC010000011        60192 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSBC010000011 DAJSBC010000000
VERSION     DAJSBC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.011"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT65_000659"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT65_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR2328312.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT65_000660"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT65_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR2328313.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT65_000661"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT65_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR2328314.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ONT65_000662"
     CDS             1642..2274
                     /locus_tag="ONT65_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2328315.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ONT65_000663"
     CDS             complement(2276..3181)
                     /locus_tag="ONT65_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2328316.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ONT65_000664"
     CDS             complement(3266..3925)
                     /locus_tag="ONT65_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2328317.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ONT65_000665"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ONT65_000665"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR2328318.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT65_000666"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT65_000666"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR2328319.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ONT65_000667"
     CDS             6168..7253
                     /locus_tag="ONT65_000667"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR2328320.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="ONT65_000668"
     CDS             7250..8314
                     /locus_tag="ONT65_000668"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR2328321.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ONT65_000669"
     CDS             complement(8563..9366)
                     /locus_tag="ONT65_000669"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR2328322.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT65_000670"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT65_000670"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR2328323.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ONT65_000671"
     CDS             complement(10244..11251)
                     /locus_tag="ONT65_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2328324.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ONT65_000672"
     CDS             complement(11258..11902)
                     /locus_tag="ONT65_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2328325.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ONT65_000673"
     CDS             complement(12002..12760)
                     /locus_tag="ONT65_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR2328326.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT65_000674"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT65_000674"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR2328327.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT65_000675"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT65_000675"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR2328328.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT65_000676"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT65_000676"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR2328329.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT65_000677"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT65_000677"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR2328330.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT65_000678"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT65_000678"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR2328331.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ONT65_000679"
     CDS             18158..19027
                     /locus_tag="ONT65_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR2328332.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ONT65_000680"
     CDS             complement(19153..20052)
                     /locus_tag="ONT65_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328333.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ONT65_000681"
     CDS             complement(20217..21041)
                     /locus_tag="ONT65_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2328334.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ONT65_000682"
     CDS             complement(21052..22347)
                     /locus_tag="ONT65_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR2328335.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ONT65_000683"
     CDS             complement(22363..22842)
                     /locus_tag="ONT65_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR2328336.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ONT65_000684"
     CDS             complement(22848..23876)
                     /locus_tag="ONT65_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR2328337.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ONT65_000685"
     CDS             complement(23893..24999)
                     /locus_tag="ONT65_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2328338.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT65_000686"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT65_000686"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR2328339.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT65_000687"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT65_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR2328340.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT65_000688"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT65_000688"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR2328341.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ONT65_000689"
     CDS             29970..30668
                     /locus_tag="ONT65_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2328342.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ONT65_000690"
     CDS             complement(30836..33475)
                     /locus_tag="ONT65_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2328343.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT65_000691"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT65_000691"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR2328344.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ONT65_000692"
     CDS             35318..36493
                     /locus_tag="ONT65_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2328345.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ONT65_000693"
     CDS             36497..37138
                     /locus_tag="ONT65_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328346.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ONT65_000694"
     CDS             37135..38052
                     /locus_tag="ONT65_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2328347.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ONT65_000695"
     CDS             38049..38720
                     /locus_tag="ONT65_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328348.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ONT65_000696"
     CDS             complement(39261..39749)
                     /locus_tag="ONT65_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR2328349.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ONT65_000697"
     CDS             complement(39950..40216)
                     /locus_tag="ONT65_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2328350.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ONT65_000698"
     CDS             complement(40216..40479)
                     /locus_tag="ONT65_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2328351.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="ONT65_000699"
     CDS             40955..41140
                     /locus_tag="ONT65_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2328352.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="ONT65_000700"
     CDS             complement(41282..43105)
                     /locus_tag="ONT65_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR2328353.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ONT65_000701"
     CDS             complement(43703..46408)
                     /locus_tag="ONT65_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR2328354.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ONT65_000702"
     CDS             complement(46714..47661)
                     /locus_tag="ONT65_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR2328355.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT65_000703"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT65_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2328356.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ONT65_000704"
     CDS             complement(48296..48658)
                     /locus_tag="ONT65_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2328357.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="ONT65_000705"
     CDS             complement(48669..49790)
                     /locus_tag="ONT65_000705"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR2328358.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="ONT65_000706"
     CDS             complement(49806..50156)
                     /locus_tag="ONT65_000706"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR2328359.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="ONT65_000707"
     CDS             complement(50286..51797)
                     /locus_tag="ONT65_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2328360.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="ONT65_000708"
     CDS             complement(52119..53471)
                     /locus_tag="ONT65_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR2328361.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="ONT65_000709"
     CDS             complement(53624..54700)
                     /locus_tag="ONT65_000709"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR2328362.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="ONT65_000710"
     CDS             54896..56641
                     /locus_tag="ONT65_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR2328363.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="ONT65_000711"
     CDS             complement(56921..57358)
                     /locus_tag="ONT65_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR2328364.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="ONT65_000712"
     CDS             complement(57342..58715)
                     /locus_tag="ONT65_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328365.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="ONT65_000713"
     CDS             complement(59037..59525)
                     /locus_tag="ONT65_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2328366.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="ONT65_000714"
     CDS             complement(59547..60149)
                     /locus_tag="ONT65_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2328367.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DAJSBC010000012        54686 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSBC010000012 DAJSBC010000000
VERSION     DAJSBC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.012"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..830
                     /locus_tag="ONT65_000715"
     CDS             <1..830
                     /locus_tag="ONT65_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HCR2328368.1"
                     /translation="LLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYH
                     VEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVAQLPLGSGVQYESSVSL
                     GYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVL
                     EQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIP
                     ARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            949..1116
                     /locus_tag="ONT65_000716"
     CDS             949..1116
                     /locus_tag="ONT65_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2328369.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(1176..1529)
                     /locus_tag="ONT65_000717"
     CDS             complement(1176..1529)
                     /locus_tag="ONT65_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328370.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            2034..2357
                     /locus_tag="ONT65_000718"
     CDS             2034..2357
                     /locus_tag="ONT65_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2328371.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            2336..2665
                     /locus_tag="ONT65_000719"
     CDS             2336..2665
                     /locus_tag="ONT65_000719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328372.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            2892..3311
                     /locus_tag="ONT65_000720"
     CDS             2892..3311
                     /locus_tag="ONT65_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2328373.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            3316..3555
                     /locus_tag="ONT65_000721"
     CDS             3316..3555
                     /locus_tag="ONT65_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328374.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            3813..4658
                     /locus_tag="ONT65_000722"
     CDS             3813..4658
                     /locus_tag="ONT65_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2328375.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            4939..6159
                     /locus_tag="ONT65_000723"
     CDS             4939..6159
                     /locus_tag="ONT65_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR2328376.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            6156..8876
                     /locus_tag="ONT65_000724"
     CDS             6156..8876
                     /locus_tag="ONT65_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2328377.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            8860..9804
                     /locus_tag="ONT65_000725"
     CDS             8860..9804
                     /locus_tag="ONT65_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2328378.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(9873..10883)
                     /locus_tag="ONT65_000726"
     CDS             complement(9873..10883)
                     /locus_tag="ONT65_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2328379.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(11063..11407)
                     /locus_tag="ONT65_000727"
     CDS             complement(11063..11407)
                     /locus_tag="ONT65_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2328380.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(11400..11834)
                     /locus_tag="ONT65_000728"
     CDS             complement(11400..11834)
                     /locus_tag="ONT65_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR2328381.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            11972..12709
                     /locus_tag="ONT65_000729"
     CDS             11972..12709
                     /locus_tag="ONT65_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328382.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12702..13913
                     /locus_tag="ONT65_000730"
     CDS             12702..13913
                     /locus_tag="ONT65_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328383.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            14117..14899
                     /locus_tag="ONT65_000731"
     CDS             14117..14899
                     /locus_tag="ONT65_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR2328384.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            14969..15631
                     /gene="trmB"
                     /locus_tag="ONT65_000732"
     CDS             14969..15631
                     /gene="trmB"
                     /locus_tag="ONT65_000732"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR2328385.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(15729..17483)
                     /gene="ade"
                     /locus_tag="ONT65_000733"
     CDS             complement(15729..17483)
                     /gene="ade"
                     /locus_tag="ONT65_000733"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR2328386.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            17615..18544
                     /gene="tadA"
                     /locus_tag="ONT65_000734"
     CDS             17615..18544
                     /gene="tadA"
                     /locus_tag="ONT65_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR2328387.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            18501..19550
                     /locus_tag="ONT65_000735"
     CDS             18501..19550
                     /locus_tag="ONT65_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR2328388.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            19547..19825
                     /locus_tag="ONT65_000736"
     CDS             19547..19825
                     /locus_tag="ONT65_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR2328389.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            19800..20270
                     /locus_tag="ONT65_000737"
     CDS             19800..20270
                     /locus_tag="ONT65_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR2328390.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            20287..20589
                     /locus_tag="ONT65_000738"
     CDS             20287..20589
                     /locus_tag="ONT65_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328391.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            20606..20977
                     /locus_tag="ONT65_000739"
     CDS             20606..20977
                     /locus_tag="ONT65_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR2328392.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            20949..21308
                     /locus_tag="ONT65_000740"
     CDS             20949..21308
                     /locus_tag="ONT65_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328393.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            21480..22487
                     /locus_tag="ONT65_000741"
     CDS             21480..22487
                     /locus_tag="ONT65_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2328394.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            22512..23696
                     /locus_tag="ONT65_000742"
     CDS             22512..23696
                     /locus_tag="ONT65_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR2328395.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(24133..25716)
                     /locus_tag="ONT65_000743"
     CDS             complement(24133..25716)
                     /locus_tag="ONT65_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2328396.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(26294..26437)
                     /locus_tag="ONT65_000744"
     CDS             complement(26294..26437)
                     /locus_tag="ONT65_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR2328397.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(26659..27138)
                     /locus_tag="ONT65_000745"
     CDS             complement(26659..27138)
                     /locus_tag="ONT65_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2328398.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(27211..27495)
                     /locus_tag="ONT65_000746"
     CDS             complement(27211..27495)
                     /locus_tag="ONT65_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328399.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(27573..27767)
                     /gene="ssrS"
                     /locus_tag="ONT65_000747"
     ncRNA           complement(27573..27767)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ONT65_000747"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27839..29125)
                     /locus_tag="ONT65_000748"
     CDS             complement(27839..29125)
                     /locus_tag="ONT65_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR2328400.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            29263..29934
                     /locus_tag="ONT65_000749"
     CDS             29263..29934
                     /locus_tag="ONT65_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR2328401.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            29900..30388
                     /locus_tag="ONT65_000750"
     CDS             29900..30388
                     /locus_tag="ONT65_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR2328402.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            30401..31348
                     /gene="prmA"
                     /locus_tag="ONT65_000751"
     CDS             30401..31348
                     /gene="prmA"
                     /locus_tag="ONT65_000751"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR2328403.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            31349..32113
                     /locus_tag="ONT65_000752"
     CDS             31349..32113
                     /locus_tag="ONT65_000752"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR2328404.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            32346..34559
                     /locus_tag="ONT65_000753"
     CDS             32346..34559
                     /locus_tag="ONT65_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR2328405.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            34570..35016
                     /locus_tag="ONT65_000754"
     CDS             34570..35016
                     /locus_tag="ONT65_000754"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR2328406.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            35187..36446
                     /locus_tag="ONT65_000755"
     CDS             35187..36446
                     /locus_tag="ONT65_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR2328407.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(36535..37482)
                     /locus_tag="ONT65_000756"
     CDS             complement(36535..37482)
                     /locus_tag="ONT65_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2328408.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            37876..39168
                     /locus_tag="ONT65_000757"
     CDS             37876..39168
                     /locus_tag="ONT65_000757"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR2328409.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            39183..40952
                     /gene="aspS"
                     /locus_tag="ONT65_000758"
     CDS             39183..40952
                     /gene="aspS"
                     /locus_tag="ONT65_000758"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR2328410.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            41061..41936
                     /locus_tag="ONT65_000759"
     CDS             41061..41936
                     /locus_tag="ONT65_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2328411.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            42202..44085
                     /locus_tag="ONT65_000760"
     CDS             42202..44085
                     /locus_tag="ONT65_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2328412.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(44160..44444)
                     /locus_tag="ONT65_000761"
     CDS             complement(44160..44444)
                     /locus_tag="ONT65_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2328413.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(44434..45168)
                     /locus_tag="ONT65_000762"
     CDS             complement(44434..45168)
                     /locus_tag="ONT65_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR2328414.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            45297..45920
                     /locus_tag="ONT65_000763"
     CDS             45297..45920
                     /locus_tag="ONT65_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR2328415.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            46112..47233
                     /locus_tag="ONT65_000764"
     CDS             46112..47233
                     /locus_tag="ONT65_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR2328416.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            47240..50368
                     /locus_tag="ONT65_000765"
     CDS             47240..50368
                     /locus_tag="ONT65_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR2328417.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            50387..53581
                     /locus_tag="ONT65_000766"
     CDS             50387..53581
                     /locus_tag="ONT65_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR2328418.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(53727..54434)
                     /locus_tag="ONT65_000767"
     CDS             complement(53727..54434)
                     /locus_tag="ONT65_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2328419.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        17892 a         9481 c        11334 g        15979 t
ORIGIN      
        1 cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac
       61 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
      121 aagaaaagta tcatgtggag atagaaataa aagagcctac agtcatttat atggaaagac
      181 cgttaaaaaa agcagagtat accattcaca tcgaagttcc accgaatcct ttctgggctt
      241 ccattggtct atctgtagca cagcttccat tagggagcgg agtacagtat gagagctcgg
      301 tttctcttgg atacttaaat caatcgtttc aaaatgcagt tatggagggg atacgctatg
      361 gctgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt tttaagtatg
      421 gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct cctattgtat
      481 tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt agttttaaaa
      541 tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa tattgtgcga
      601 acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa atccctgctc
      661 ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt agtgtttgtt
      721 taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag ccccgtcgtc
      781 caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag tgtattttat
      841 gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa taaagatttg
      901 aattaaagtg taaaggagga gatagttatt ataaactaca agtggatatt gtgtcctgta
      961 tgtggaaata aaacacgatt aaagataagg gaagatactg aattaaaaaa attccccctc
     1021 tattgtccga aatgcagaca agaaaattta attgaaataa agcagttcaa agtaactgtg
     1081 attacagagc cagacgcaaa gacgcagagc cgataaaatg agattaatac aatctcattt
     1141 tatcggctct ttccgttatg tatggattct tttaattagt cttcgatgtt tcttgcttcg
     1201 ttgataccgc tggctaaaga ttccattaag gatagttctt tgtctgtaaa gctatccatg
     1261 tatttctcta tctgtaatcg tcgggtgctt tttaccaagt tattagcagg taagaaaaat
     1321 tcatcaacgg aaacatgaag taacgataca aggtcataaa gaacttgtat gctggggtgt
     1381 tgccctttat tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa tgctcccact
     1441 tgttcacgag ttaaacctcg tttcaatcga gcttctttaa tggctaaacc aaaggctcta
     1501 aaatcatatt tatcttcttt tttacgcata gtagaccacc tctatacatt ttattgttcc
     1561 tactgaatta aaaacaggta tagaaaaacg tgttatatgg tttataggtt tatatttaat
     1621 aaaaagcact actaaacgcc aataaaaaaa accgttatat ggtagtgcta tttacgctgt
     1681 taaaatattg tatattactt ccaaatggcg gtttgttgga ggtcaacgtc gccatgaagt
     1741 acatcatata caataaattt ccttacattg ggttcttgtc aaaaaaagtc gtctatctgc
     1801 aatagataag tacgtccacc aatgtggttt tataaatcat atagatagaa taacagaagc
     1861 atgtaaacag agaaataaat ctgtttatat gcttttttgg ctattcagaa cttttttaca
     1921 aagtttattt atcagtaatg caacaaatcc ccctttcaca ttgggactaa gagtgaaagg
     1981 agataaacga gcaaggctca cttcctttcc tagacagaaa gggggtgaga aacatgaaac
     2041 catcttcttt tcagaccaca atagaaaatc agtttgacta tatctgtaaa cgtgctatgg
     2101 aagacgagcg aaagaattat atgctttatc tttcaaggat tgcaaagcgt gaggtgtcct
     2161 tttcggatgt tggcgattat cttgttagcc agtttgcgac aacagataac tattcaactg
     2221 actttcagat ttttacactc aatgggttat cagtaggcgt tgaaaatgat ttgttgagtg
     2281 aagcattacg tgagttgcca gacaagaaac gtgaaattct actgctgttt tacttatggg
     2341 aatttcgcta tttttagaat tgcctgcaat agtttcctac tgggtagtta cagtgatgtg
     2401 ctttgtggct atgggtgtat ctactatgtt tgttgttcaa atttacactc ttgttcagac
     2461 acaaacacca ccgcaattag taggaaagat aatggctacg ctggtagcag ttgctatgtg
     2521 cggtcaacct attggacaaa tggtttacgg tattttattc gatgtattta tacaagaaac
     2581 atgggttgtt atgtttgtag cttctattgc ttcaatcatg atttcaatat attcaaaaag
     2641 aatttttcaa aagttagaga tataatacaa ggggttaaat agtatgttag gctaaaaaca
     2701 atatataaaa gaaaatttga aatctgctaa aaggatagtg ttataaaaga aagagtaata
     2761 ttgcagtatt taaggctcgc acaaatatat gttttgtgtg ggcttttttc ttaccatttt
     2821 tctgtatttc taaagggtat aaacggtatt ccgtttatat atttcagcat tagcagaaag
     2881 ggggtgagat tatgaggaca tcttctttcg agcatatcgt cagattacag ttcaattctt
     2941 taatgctgat tgtcattaag aacaaagtga aaagtcgaaa cagacagctt gcaagacatt
     3001 ctaaacgtga agtattgttt tgtgaaatag ccgaaacgaa acaagttgaa cgtggaacga
     3061 ttgatacctg tttttatgat tgtgtatcat ttaaagtctt gcactttaca atttatgtat
     3121 ctgatgaaac acttggtaca gttttacata gactgtcaga aaaacagcgt agtgcgattt
     3181 tattacgtta ttttcaagga atgaacgaca gaaaaatttc agaactgtac catgtatcac
     3241 gttctgctat ttgcagtaga agaactagag gactaaggaa gttaaaaatg cttttgagcg
     3301 aagggaagta aagaaatgag taaagattat ggctatccct cttttgaatt gatttgcaaa
     3361 gcgtcaagcg gagatgaaat ggcagttaaa gagattttga agttttatga cggttatatt
     3421 tctaaattat gcttgcgtcc tttttaccac tctgaaagtg gtaaaatcat tatgcaggtt
     3481 gatgaagaac taaaaggaga aattcataca gacatgatga aagcaatatt gaaatttgaa
     3541 atcggagtga agtaatcaac tgtataaaaa aatcgggaga tacacagttt cgtgctatct
     3601 cccttagtag ttttacggtc atatcaaggc tcattcaatc gtggtaaatt cgtggtaaga
     3661 tgagtgtttt tctatacttc aaaatgcttg aatgtatagt gtttatgcgg ataattgaaa
     3721 attagatata aaatttctta caattagtat atgttttgtg agaaatttag acttacaata
     3781 agcagtgaat ttataatcaa cgtataggag acatggatga aatgaaacta attggtatag
     3841 atttagacgg tactcttttg aattctgacc agagcattag tcaaaaaaat gcaagaacaa
     3901 tgaaacattt accggaagac tgttttccat tcatttgttc tggaagagaa gtagaagaca
     3961 ttaataatat tctcaaaaaa tctggtttaa ggataccagc agtagggtta aatggttctg
     4021 ttggatttga tggagaaaaa aagatatttg agtcttcctt cgataaaaat gatgtaaaaa
     4081 aaattgattc tgtcctatcg aaatttccaa ccaaaatata tacaaatctc ggaagttatg
     4141 aatctcagaa ttataaaaat aaaatgaaaa aaatttttca cgaggtaggg agtgaatttt
     4201 ctatcaaaga gctaaactat gagcttgagt atgaaaaatc tatttgttca atcccttata
     4261 ataatattga ggaaattata gaaaaggatg ctatcagcat ttacaaattt tttattttta
     4321 ttcctaatcg tcaattgaaa gctaatatat ttaaccattt gaaaaagatg ctaaatgtgt
     4381 ctgttactga atcggcacct gtaaatattg aaatcgtacc tcaaaatgta tctaaaggtg
     4441 tggtgtttga tcgtttggaa tcaatctaca atttaaagaa acctttgaga atcgcgatcg
     4501 gagatagttt aaatgactta tctatgtttg aaagtgcaga tattagtttt gcaatgggaa
     4561 atagtcatca agaaatcatg aaaaaagctg attatgtcac tgtttcaaat gataaagacg
     4621 gagtatctca agcatttgaa agaattctta ccatataaac taattaaggt agtatagaac
     4681 atagaattct aaatacggaa aaatggagca taaattccag tacaaatagt acttcttcag
     4741 tgtcaaacga agcgaatata acatagatat ttaggattgt gacaaaaatc atgtcacaat
     4801 ctttttttcg aataaactta ttttttgaag tcggccattt atcgtaattt ctttgcataa
     4861 tacgaacaaa ttttcggtta ttgatgaaaa aaaacattga aaatggtatg ataaaagaaa
     4921 aaaaggagga aatttttaat ggtgaaattt atccatgcgg cagatttaca tatggatcgg
     4981 tcatttgaag gactgacaac tcttgataaa actgtacagg aaaaactatt gaagacgaat
     5041 ctgaccgttt tatcaaatat cattgaacag gcaataataa ataacgttga ttttgtttta
     5101 cttgcaggag ataattttca tcaaaatcgt ccttcattaa aaatacagaa acatttttca
     5161 gaacagatga aacggttaga aaagaaccat atacctgtat ttataatatt cggaaatcat
     5221 gatttctatc aaaaagagcg gtattggttc tcctttccta aaaatgtcca tttatttaca
     5281 tccgaaacgg tagagacaaa aaaaataacg ataaaaagtg gagaaactgt ctctctcagt
     5341 ggatttagtt atcgtcaacc gtggattcaa aaagacaaag tgatggaatt tccttctaga
     5401 gaactcacag attaccatat tgggttgtac cacggggaac ctggcgtctc tcaaaaagga
     5461 aactatgctc ctttccaacc ctcaaagatg caagaaaagg gatacgatta ttgggcttta
     5521 gggcatatcc atgtgccaac ggtcttaaat gaaaagcaaa caattgttta tccaggtgcg
     5581 cctcaaggtc atactaaaaa ggaacaagct tctacatcta ttctactagt tgaattaagt
     5641 aaaggaagtt gccaaataca tccgattaaa gtagcagagg tctattggaa gacaaaggaa
     5701 atatcattga ggaaagcaag aacgacaaaa gaggtcattg ttcacattcg tcagcagctt
     5761 tcaaaagtag aggatggatt tagtttaatt gagataaaat taaaagatta tgaccatctc
     5821 aatacagatg ttttggaacg gatacaatca ggagagctat tagactattt gttagaggaa
     5881 ttctcagata ggataaatga tttctttatt tggcgtattt ctctcataga gaatactttt
     5941 tatacgaaga ccccacttgc tgcttctgct aaattgatgg aacagttatt tcaatactat
     6001 cagacacctc aagatttcca acaaatttta aatgaagtat atagtcatca agaagcggct
     6061 agaatattga gcgaactgcc ggaatatcaa gaagaaacac ttgaaaaagc caagcagctg
     6121 cttaatcaag attttttatt tgaggaggat caggaatgaa gattttaagg atcgagattg
     6181 ctgcttttgg aaaatggcgt caaaaaagtt ttgactttta ttcaggcaat cagctgatct
     6241 atgggggaaa cgaagcagga aaatcaacga tttatcaatt tattcaagca atattatttg
     6301 gtttcccttc aaaaggccgt aaaaaaaagg attatacacc aaaagacggc tcagcctatg
     6361 gcggcaaaat atggcttaaa catcctgtat atggtgaatt tgctgtcgag cgctacaagc
     6421 agcaaaatcg cggaaaatcg aaggtgtggc tgggggatca agtgggaagt gatgagttac
     6481 tagaaaaact gatcgcacct ttgacgaaag agttattcca gcaggtattt actttccagc
     6541 aagagcagtt gatggaacta gatcatttga gagaaaaaga gctacatgat gcgctgattt
     6601 ctttaggcat cacgggcagt tctcaagtat tccaaaagcg gcaagaatat tatcagaaag
     6661 cacaacaatt attcaagaaa aaaggccaaa aattactaat caatcaaaaa ttaaatgaat
     6721 ggaaagagct gcaagaaaaa attcatcaaa aacaagaaca ggaagcttat tttggcaagt
     6781 tgatccaaca agaacaggaa tacgagtcgc aacagcagca gttattggca caattaaaag
     6841 aggcgaacaa acaattttca ttagccagac agcaagaact taatttttct ttatatgaag
     6901 aatggcaatc tctcaaaaaa aacagcacta caggtcttcc tgatgagcaa cttcttttag
     6961 atctggagtc tttctccaaa cggtatcagc aaacaaatga gcgtttggaa gaattagaag
     7021 caacactgga aaagcatagc gggatggatc agcagtcagc cagatattat ttttatttag
     7081 agcaggagca ggaactaaaa gatctccagc aacttcaatt tacagcggaa caattaaacg
     7141 aagaatggca aagattacaa attgagacta gagaaacaaa ttcagaattg aagattttgg
     7201 aacaaaggtg gcattggcag aaaaatccgt taccggaaga atttttatat gaagaagaat
     7261 ggaagcatct tctggctgaa gaaagaagga ttgctgatct agaaagccag atttctgtgc
     7321 gtctccaaat agctaaagaa cagcaagaga tgattgaagc agaagtgaat gacttggaag
     7381 agacctatcc agcattatta caaaatcagt cagagatagc accagtctct tcatctaaac
     7441 cgtatatgct gatggctggc ggagcaataa tcattgcagg tatatttgta ccaatgccct
     7501 tgaaagtgat ccttttacta attggtgcag gcatgattgg ctatttcttc tacaaaaggc
     7561 aaccaaaaat taaaactaaa aatgatttct ctgagataaa aggaatgtgg caagaaaaac
     7621 taggacaact tgatctagcc ttagctcaat tagctgaagc agaagaaaaa caaaagagaa
     7681 ctggagaaga aaaacagatt cttcagcaag aagtatatcg ttttgcggat catcggcact
     7741 taggaaagat ggatacgctc aaactcatga aagaatatgg acaggaagtc atagattatc
     7801 agaaattata tggcgtattt gagcggaaac ataaaagaga acaggaaata caagaaaaac
     7861 tacgacaaat cgaccaaaaa tttgagtttg ttaaagaatg gttaccgata caagataaga
     7921 caatttcaga aaaaatggta ttgctttccc gttttataca agagatggaa cagatgaaat
     7981 ttgccagaag ctatcagcaa aatactttat tgaagcaaga aatcaatcaa ttgaaaaaga
     8041 aacaaaaagt atggatacaa gaatacagcg aacaattgac tcaagcaggc ttatcttatc
     8101 cttcggaagt acctgtcttt ttacaaaaag ccaaacaagc cgaacagcag atgaaaagat
     8161 acaatgaatt atcggaaatg cttactccac tatttcctga agagctgaca aaagagaaat
     8221 tatctgtaag actgcgccaa ctgaaagaaa agcaatccca actccagcaa gaagaaaaag
     8281 aagcaagtga aaatcggcaa cgactccaac ttcaagtaga agagttgcaa agagacggca
     8341 cattggacga actttatcaa caagctagcc ggcaaaaagc aaaattacag gaattactgg
     8401 aggaatgggg agcacttgaa atcgccggga cattcttagg tgatctttca acagagatga
     8461 gtgaaagaca attaccccag ttgttaagag cagcaggtga ttatttaggt attttaacaa
     8521 aaggaaatta tcagaagata ttactgaaaa atgatacact gactttaaca gacggatata
     8581 cagaatttcc catctatgag ttatctacag gtacgaaaga tcagctgatt atggcgatga
     8641 gatttgctta cctctcatta gaagctaacc gttcgatatg cccgatcatc attgatgatg
     8701 gctggcttca ttatgatcat cagcgaaagt atcaattagc tgaactttta gctaaatttg
     8761 gagaaaagta tcaaataatt tgtttttctt ctgataaaga aatggtaagc tattaccatg
     8821 agatgaacca aatcgtaaaa caattagaag gagcaaataa tgaaaaaaat tcgtgattta
     8881 gtaatcgatg agcagtttga acaatttgtc ctgatcaaaa gtgcagatgt aagaatcgct
     8941 aaaaacggaa aaaaatttat tgcttttact ttccaagata cttcaggtac tatcgatggt
     9001 aaatactggg atgcttcgga ggaggaaatc aataaattta cagccggcac agtcgttttt
     9061 ctaaacggta aaagagaggt ttatcaagga aaccctcaag taaagattct tcatatgcgc
     9121 ttaacaaggg tagatgagcc aggagagcct agtttataca tggaaagagc ccctttgaag
     9181 aaagaagaaa tggaagaaga aatcaatcaa ttgttgtttg agatcaccaa tgcgcattgg
     9241 aatcgtattg ttcgctatct acttaataaa tatcaaaaac aattttttga gtttccggca
     9301 gcaaaaagaa accatcatgc atttgccagt ggattagctt atcatacttt gacaatgctt
     9361 cacttaggga aatcaatcgc cgatgaatat cctgagctga atcgagcatt gttgtatgct
     9421 ggtatcattt tgcatgattt aggaaaggtg attgaacttt ccggtcctat gtcgacagaa
     9481 tatacgcttg caggaaattt gatcggtcat ctggtattga ttgacgaaga aataactaaa
     9541 gcctgtcttg cgttgaaaat cagcgaagct gacgaagatg ttcttgtatt acgtcatatg
     9601 gttttagcac accatggctt attagaatat ggctcacctg tacgtccaag aataatggaa
     9661 gcagaaatat tacatcaaat cgacaatatc gatgcttcga tgcagatgat gttgacttcg
     9721 attcgtcaaa cagaaccagg aaaatacacc gatcggattt tcggtatgga caaccgaagt
     9781 ttctatgtac caaaagattt atagcaaaaa tttatttttt tcattcaaaa caaaaaaagc
     9841 gagacataag tcttcgcttt ttttgttttc ttttattttg atgaatcagt tgtttcttca
     9901 gttgaagatt cttttgttga atcagttgat tttgtgtcag aactctttgt tgaagctgta
     9961 gtttcacttg agtctttcgt acttgatgaa gttgttgtga atgcactcaa cacattttca
    10021 aaggcatcat ctttgatttt tacatttgca tcttttaatt cttcaccgat tactttagtt
    10081 gtgaatgcat tgtcacttag tttagtttca gtagcgatat cttttagttg atctttgtat
    10141 ttatccatgt cattgccttt attttggttt ttcaccattt tgacaacgta gtattctgtc
    10201 gcataagaag taggatttgt tgtagtaata acatcggaga tctcaccgtc tttcaatttg
    10261 aaagctgctt cttttacttc tgcaggaata gttgttgtcg ttgagtcgaa tttaacttta
    10321 ccgccatctt cttttgtctc tgtatcagtt gatttgtctt tggctaattt agagaaatca
    10381 tctccatcat ccgctgattt tttcacatct tttgcttcat cttcacttga taatttgatg
    10441 atttgtgctt ctacttctgg atggaaagat ttccaagcag ttttcaaatc gtcatctgta
    10501 atatctacgt gagctttcaa gccagcttca aaagctaaat tgttacgaat atattcttta
    10561 taagtatctt tcgtatatcc agcagcttct aattgtgatt caaaagtatc acctaatgat
    10621 ttcgcttgtt tgtcgtattc tgcatccact tgcttatcgg ttactttatc gccatatttg
    10681 ttattgaata ctttataaat gatcatccgt tgtactaagg attggttcga tgattcaagt
    10741 tttgcttcat catagaaatc agatacagtg atcgttccgc ctttcatcgt tgcgatatct
    10801 ttattagtat cgcctgaaca agctgctaga gttaaaacag ccaacgcact agtagctgcc
    10861 aaaataattg attttttttt cattaaaatt tgcactcctg taattagtta ttatttttat
    10921 atattaacta taccatatcc catttccttt aaaaacgttg tcgtggtagt ttcaaacaaa
    10981 attcaaaatt tcatcacatt cctaggaaat acgcaaactt ttctcactta tatagttaaa
    11041 tctgttgaga aaataatcat tattatattt gttcaggata ctctggcaga tcagctttta
    11101 gtttttctac ttgttcattg atttgtgtga tacgtggttc ggcttggaat ttaaaatctt
    11161 cgatatcttt tttgattgct tcttggaaag caggaatcac agattcagct gtttcttttg
    11221 tttcgatcaa tgcgctttta aaatgattca ggctgttatt taaatcattt gtcagagttg
    11281 ttgcttcttc caattcttcg atcagtttct ttcttgtttc attcccgctc ctgggcgcca
    11341 ataacagacc tccgacagtg ccagcagcag ctccaaataa cagtcctttg aaaaagtttt
    11401 taatcatcgc aggacacctg ctttacgatt gtttcttgaa tagcttttag ttcttccgat
    11461 ccataactat cttgatgatt gacaaagtga atagaaaaat catcctcttt gctgtagcga
    11521 gggactaaat ggatatgtga atgaaaaaca gattgatagg ctagttcacg gttgttattc
    11581 aagatattaa gacctttcat ttctggaaaa gctttttcca atgcttgagc aatcttaggc
    11641 acacgcgaaa aaacctctcc tgcaagttcg ggatcatatt caaaaatatc tgctacatga
    11701 cgtttaggca caactaatgt gtgtcctttc gttgcttgag agatgtctaa aaaagcatag
    11761 accttgtcgt cttcatatat tttatagctt gggatttctt gattgatgat tttgcaaaaa
    11821 atacagtttt ccatataaaa catcctttct ataaagaata aatacttcag cttaacttta
    11881 ccataagtta aagctattgt aaggaaaaaa acaaaatacc gtcattttct tcgtggtata
    11941 ataaataaaa ataaatggcg gaggaaaaag aatgagttta attatcgatc atgtcacagg
    12001 ggggtacgga catctccctg tgttaaaaga tatcagtttc gatgtacagc ctggagagat
    12061 agtagggttg attggactga acggagcagg gaaaagtacg accatcaagc acattatcgg
    12121 tctgctggaa gcttcaaaag gaacgataga aatcaatcat ctatccttaa agaaaaatac
    12181 ggaagaatat cgcaaacaaa ttgggtatat tccagagact ccttctttat atgaagaatt
    12241 gacattgaga gaacatatcg aagtcactgc gatggcctac gatataccta tggatatagc
    12301 aatgaaaaga gcagaaaagt tgttggagac ttttcggtta tctaatcgtc ttgactggtt
    12361 cccagctaat ttttcaaagg gaatgaaaca gaaagtgatg atcttatgcg cctttttgat
    12421 cgagccttct ttatatatca tcgacgaacc atttttaggc ttggatccac tagctatcaa
    12481 tgcattgctt gaattgatgg tagaaatgaa aaaagaagga gccggtattt tgatgtctac
    12541 gcatatttta gctactgcag aaaaatattg tgatcgtttt gtggtcttac ataacggaga
    12601 gatacgagca gacggttctc ttgagatgtt gagaaaagaa tttcagctgc cggattcttc
    12661 tttggacgaa atctatattg ctttaacaaa ggagcaggca catgagtagt ttttttcaaa
    12721 aacgtttagc aagacatcaa aagaaaatga tgaaatatat gcgttatgta ttgaacgatc
    12781 attttgtttt agtttgtctt ttcttgattg gcggtgtggg attatattat tctaattggt
    12841 tgaaaaccct gcagccaccg tttgcaattg gagggctcat aatctctgta ttttggatgc
    12901 tctgtttatt cgtgggtaag atggctactt ttgcagaggc agcagatatt gtttttttac
    12961 ttcctaaaga aaaagaaatg caaacctatt tggaacgagc ttttcgctat tcttgcattt
    13021 ttccgttgat ctttttgata ttggcatgcg gattggccat gccattagtg gtggttagta
    13081 cggagcaatc cttttcgatg tttttcgtct atctgatcat attatggagt ttgaaattca
    13141 gtcatctgca aatcaagaga gcagaattgt ttcgactaga tcaacaagtt attcgttatt
    13201 ggaaattagg ctggtttttt tcttctctga tctttttgtt tctatcattt tattggctaa
    13261 tatgggcagg aatagcaggt gcattagtac aggcaagtct atattactgg tttttgtgga
    13321 agcaacaaga aaaaggattg gattgggaga aaatggtgca tgtagaagaa cataggttgc
    13381 atcagatcta tcaatttatc aatttattca cagatgttcc tgaaattacg gcaaagatca
    13441 aaagaagacg ctatttggat cctatattga ctcatattaa aagagaatca aaaaacactt
    13501 atctttactt gtttgcaaga agattggcaa gaggttctga ttttagtggt ctttatttcc
    13561 gattagtaat tataggtggg ttattgattg ccggagtagt cgacttttat tttagtctga
    13621 ttatcggttt cttattttta tatttgatcg gtttccaact gttaccttta gcgaaccagt
    13681 tccgctatat ggttctcgtt cagctttatc caatccataa gaatcagaag aagcaggcga
    13741 ttcagtatct actcacatgg gtattaatta tcgcgtcggt tatttttggt ttgattgctg
    13801 cagtcgtatt agatgggcag cagcgatggg ttccgctagc ggcttatttg attgttacta
    13861 tgctctttgt taaactatat gtaccaactc gtttgaagaa gatgtcagac tgaaaacaaa
    13921 gctgtgtagg gtaaaaatga gaatgaaaaa tagagcgttg acagtaagaa tgctgttcgt
    13981 tctatttttt ttgagaaaag ctggtttttt gaatggcaat taaatgaaaa agaacgtata
    14041 tgtgcttgac gtgtagtgtt caggcgacta gaataataag tactgcaaaa caaagagtaa
    14101 gaggacggga gaatgcatga tggagtttca gttagaccaa gagtggcggt tacgtcccat
    14161 caaaggcgac acaggaaaaa catacatagg catgcgtgat gaggacaaag tctttattaa
    14221 acgaaacacg actccgatgt tggctgctct ttcaagggaa ggaattacac caaaacttgt
    14281 ttggacaaaa cgaacgggga atggcgatac tttgacagca caagagtggc tggaaggtcg
    14341 cttgctgact cctaatgaga ttggtcaaag aaacgacgtg attgatgttt tgtaccattt
    14401 acatcattca aattctctta aaaccatgct gacccgtatc ggtggtcgtg tcatgacgcc
    14461 ggagaagatg ctgcaaaaat atgaaaaaga attaccggaa gaactccaga aaaatcaatt
    14521 tctgcaaatt gtccatcgtt ttcttcgtga gcagatcccg tcaatagata aggaaaactt
    14581 atcagtagta cacggagatg taaaccatcg taattggtta gtatgtaaaa attatttata
    14641 tctagtcgat tgggattcaa tcatgtttgc agaccctgcg gtggatattg caacgatttt
    14701 aggaaattat gtgccactgt ctagctggaa tcagtggctg atcagttatg gcatacgccc
    14761 gactgacgaa atgcttgaaa aatttcattg gtatgcggcc atgaatttgc tccaagaaat
    14821 caccagatat tgcaaacgtg aagatcatcg acggatgaac gaagaaattt tacaattaaa
    14881 acgaattttc agtggctgag gtttggccca aactctgcgg agatttggcc agaccttttt
    14941 aattagagga aaggaatgga gataaataat gcgtgtccga aatcgtccgg gagcagcgga
    15001 aatgttagcg gctcacccga attttgtaat tagtgatcca acattgtgga agggaaaatg
    15061 gaatgaattg tttgaaaacg atcatccaat ccacattgag attggtatgg ggaaaggcca
    15121 gttcatcact ggaatggcaa aagcccatcc tgaaatcaat tatattggag tagaaatgca
    15181 ggtcagtgtt gtttcgattg cactagacaa gttgatcgaa caaccgcttc ctaacctgaa
    15241 acttctacat gtggatggat cagcgttgac agagtatttc gctgacagcg aagtagatca
    15301 aatctatctg aatttttccg atccatggcc aaaaaaacgt catgaaaaac ggcgattgac
    15361 atataagact tttcttgctg tagatgaaca gattcttcgt ccaaatggag aaatccattt
    15421 caaaacagat aatcaaggat tgtttgagta ttctctggca agtttttctc aatatggcat
    15481 gatcctcaaa caggtatggt tagatttaca tcaaagtcaa tttgaaggca atattatgac
    15541 ggagtatgaa gaaaaatttt cttcaaaagg tcaacgtatt tatcgagtag aagctcaatt
    15601 tcaagataag accaaaaaga aaagtctgtg aaactaactt tctgctttca taaaaacatt
    15661 aatatccata aataaatctc agaaaatgaa cattacagat gtaatgtatt ttctgggatt
    15721 tatttgattt aaaatgtatt tatttcatcg gtttttccac aaggtggaat caattggaag
    15781 cgagaaaatg aaaataatcc tttgtcggtg atcttcaatt cgggtatgac agataatgtt
    15841 aagaaagaaa gtgtcaaaaa aggatcaaag gttttagctg ctcctagttg atgtgcttta
    15901 gttattaatt cattcaactg ttcatagacg atcgaaaaag gttcctgtgt catgattccg
    15961 cctataggaa gagaaacaca agctatttcc tgctgtttgt tgacaacggt catcccgcca
    16021 cctttttgaa tcaaaagatt agcagcatac agcatatcct cgtcgtttgt cccaacgaca
    16081 actagattat gcgagtcatg ggcaatcgtt gtcgctatcg ctccttgttt cagctgaaat
    16141 cctttcacta tgcctaatcc aatgtttccc gtttgatggt gacgctcgat caccgcaatc
    16201 tttaattggt ctttttcgat tgaaggaatg aatcgttcgc cttgtcggtc cactttttct
    16261 accaaatctt tagtcactag actattcggg ataatttcga tcacatgggc ttgtgaagaa
    16321 gaaagaagca gctctaacga atgtggattt aatggttgag cgtttagttt tggcagacaa
    16381 cacgtatatg aatttgaatg atcagcaaat aaatggtctt ggagttgccc agattgaaca
    16441 actaatgttc ccttatagaa aacttcatga attggcagct tggtaagatc gtctgttaag
    16501 aagaaatcag cttgataccc aggggcaatc gcacctagtg ttttcaaatc aaaacattct
    16561 gctgcattca atgttgccat ctgaatggct tgtaatggat cgattccaag tgcaatagct
    16621 tgacggacga tatgatcgat tgatccttct tttactaaat cattgatcag tttatcatcg
    16681 gtgacaaaga agcatcttct tgaattttca ggtgtgatcg ccggtagtag cgatgccaga
    16741 tcttttgcaa cggtcccttc tcgaaccatt aaatacatac caagatccaa acgttcttta
    16801 gcttcttttg cagttgtagc ttcatgatcc gtccgaatac ctgctgcagg ataaacattt
    16861 aactcttcca tgccgatacc agctgcatgc ccgtcaattt ttttcttatg ctggtgcatc
    16921 aattggatct tttctaggat gtctgtttca ttatttgcta ctgcttgata gttcatgact
    16981 tcagctaagc ctaaaacttc gggtttttgt aaaaaagggg ctaatttttt tgcatttaaa
    17041 tttgcaccat tggtctcaaa aggggttaca ggtacacaag aaggtagcat aaaaaagata
    17101 ttcatgagtg tttgtcttgc atcttctatc ataaactcaa gacctgtact cccagcaatg
    17161 tttcctattt catggggatc tgtcactaca gtagtaactc catgaagcaa ggatactttt
    17221 gaaaattcgc ttggtgataa aagtgagctt tcgatatgca catggccatc gatgaatcct
    17281 ggcactagat attgcccatt agcattatgt acgctttttc cttcgtattc accgattcct
    17341 acgatcattc cttcgcagat tgctacatct ttttccatga tttctttcgt aaagacgttg
    17401 acgatcttag catgcttgat cactaggtca gcaggctttt ttttacttgc tacttcgata
    17461 tatttcctta atgttgtttc caatggagtg cctcccaaga taaattttta taagtataac
    17521 ataaattcgg cttttgccaa tatttttttg taaattcatt ttatttgaaa caaaaagatc
    17581 aaaaagacgt atgttttttg tggaggggag aataatggaa ataaaacgat tatcggatca
    17641 aatgataagt gaaagttttg ccaaacatgc ccaagacgta tatatattgc cagcttcaga
    17701 agtattttac atctattttc gctatggaaa aaaacgaaca ttctggaaga aagtctcttc
    17761 ggcagatgga cagcaactga tctcaagatt taagtatttg ggacagatgg atatcggaga
    17821 aaaaagaaaa gctcagttag gtgcatttac ttattcttta gaaaaacagg aagttcgtct
    17881 gcggttgtct tctgtaggag attaccttca acgagagagt ttagtgattc ggctattata
    17941 cggtactttt gaaacacccc atcgttgctt tagttctcga gattttgcaa ctattttaca
    18001 gaaaaccgtc agacgtggat tatacctttt cagcgggaca gtcggatcag gcaagacttc
    18061 tctaatgtat cggttggctt tggaagaaaa attgcaagtg attactatcg aagatcctgt
    18121 agagatagaa gaaccgtttt ttctgcagct acagatcaat ccgaaaatag cacagactta
    18181 tgatacactt ttgaaactcg cattacggca tcgtccagac ttgctaatca ttggcgagat
    18241 aagagatgaa tcaactgcac atgctgcggt aagagcagca ctaacagggc accgggtttt
    18301 tgcaaccgta catgcacgca atcttgaagg tacattggtc agaatccatg aactgacaaa
    18361 gaaaaaagaa gagctatctg aatgtttagc tggtgtcatt tatcaagaac ttttggaaga
    18421 tgccaatcac catccttctg ttttatggag ttatcaattt atggaggaag aatcagtaaa
    18481 aaaatcatgg gaggaaagtt atgaagaagc tcaggaaaat atttggcagt cagaactatt
    18541 ctaaaggatt aacaagaagc cagcaaaacc agtttgcaca aaccatggga gaactgcttt
    18601 tatctggttt tagtctgcaa gaatcccttt atttattact cacgatccgt tcgtttccac
    18661 ctgttatttt ggcaaatatc caacatcagc taaaggaagg gcaagcgttt agtgaggtac
    18721 tttcgcaaat aggatatgga gcagagcaac tggtacagat cgagttagct gaacgtcatg
    18781 ggaatctgcc gacaactttg ttggaaattg ccaaacagtt tcggcttgtt gaaggatttc
    18841 gcaatgattt gaaaaagctg cttgcatatc cgatgctatt gctcaccttt ttgctaggta
    18901 tcttgtttag tatgcggttg ttcgttttgc ctcagcttat tggttcagga atggtcatgc
    18961 aagagcattg gggtattcag ctgatacaag tctttcattg gtatgttcta ctattcatcc
    19021 tagcggttac tctattggct ttactagtaa ggtatcgtct gatgaagttg tctcctgttg
    19081 atcagagtga atggataagc agacaattat tgattggacc gatatattcc ttgtatcaat
    19141 cggcttattt gtcactagag attggaaagc tttttcatga aggtttcgaa ttaaggcaaa
    19201 tcatagtctg cttaaaggaa acgaaacaag aaagtctgat tcagcatctt gcctctaagt
    19261 tgattcaagg attggaggca ggtatgtcat tggctgagca gttccagaat tatcgttttc
    19321 tatcagaaga attcagtaaa attgttttac agggagaggc aaagggaagt ttaggtaaag
    19381 aactgatttt ttacagcgaa tggacaagaa aacagctttt caaacgtctt catcactgga
    19441 tgcattttat tcagccaatc atttttttgg gcgtagcggc tttgatttta ttagtttatg
    19501 ccgctgtttt attacctgtt tacggaagca tagaggaggt tttgccatga aaaagagaaa
    19561 aatatcttat tatagcggtt ttacattgat cgaaatgcta atcgtcttac tgatcatcag
    19621 tgtattagtg ttgttgttcg tccctaattt gtcgcgttat aggaatcatg tagaccaaga
    19681 aagtcgtgaa gcgatcatcc agttggtaga cacacaaaaa gagctctatg cgcttcaaaa
    19741 taatggaaga gttcctactg tggaggaatt gttgaatgaa ggctatatca aacgagaaca
    19801 tgcagaaatc tatcagcggc cttgaaggta aggttttagc atcaggatat acgttgtttg
    19861 aatcactctt actgatttta ttacttacaa cgatcctttc ttttccagtt ctcgcatttt
    19921 ctgcatggaa gaatgaattg gcagtctatc aatttttcgg ccaatttgaa aaacgaatct
    19981 attctacgca aaaaatcgct attgtgaatc agatccaaac aggcttttat tggaatagtg
    20041 aagagaatca aatcattttt catgttccga atccagataa aataaattgg gaagtattag
    20101 atattcctga tgagattacc ttgaaacgcc atgcttctat tacttttgct gctagaaccg
    20161 ggaatgaaag ttcactaaaa gcttatcaat tttattggga agaaaaagag cggacgatta
    20221 cgtatcaatt ccagataggg agtggtcgct atacaaaaag aattgaataa aacaggattt
    20281 gttttgttgg agtcccttac agcgcttatg atcgcttctg tctgtgtcta cttagtgagc
    20341 tttggccagc tgtttttgct tcaggcagaa aagaaaagtg gggaggaact gctatttatt
    20401 cgaatgattt atgaagatat caagacgcag cggatgtatc acaaaagacc agagcgaagg
    20461 gaagaaatga tcccatacga tatccaaatt gtcgataata atggagtaat cgaaaaagtg
    20521 tttgtcaata tggagaaaga agcgatcagt attgaaaaaa caagtaattc cagcatttac
    20581 gctgattgaa tgtctaattg gactgatgat attgagcagt ctattgctca cattcaatct
    20641 tttgattcag caaaccatcc aagtaagtga atttttgcag cgtaaagatc aaaaagagtg
    20701 gctaatattt ttagcacaat tagaagaaga actcaagagc agtcacaatg tctctgtgaa
    20761 aaatcagcaa ctgcactaca cgatcgagga taaacagtat atcatcgaaa gatatcaggc
    20821 aatgatacgt aaaagaagaa cttccggcgg acatcaacct atgttaacaa gtatcactga
    20881 gcttcaatta ttggaaaaag aacacacaat ttctttttat gtacactttg aaaatggaga
    20941 agatggatat gccatatgga caaaaaacga tcagtagaaa atgttataag ggctctattt
    21001 tattttctgt cttgctttta tttcttttat gtacgttttt attgatggca gcattagaaa
    21061 gctaccgttt gtcaatggat cttaatatac gaatcaaaaa ttattatata gcaaaaataa
    21121 tgaagcagat ggctttatcg gaacagtcta cgcttgccga aagttcggaa ggagtttttt
    21181 actacacaac tggatctgta acatatcagt gggtacaaca gtccctattc ttagaggtgg
    21241 aagtctcacc gtttatcttt cggttcacag aagaagtaaa aaatgataca gatactagta
    21301 cagaatgatg gaatgttcgt gaaaacgccc tcttttctgt tataacactg tttttaataa
    21361 aaaaatgttt gccgtttcac aaataaaaaa cagtgaaaac gattgaaatt ttgtcaattg
    21421 ttcgatagaa ttaaacaggt gaaaaaattg ttataatata tctagaaatg aggtgcacca
    21481 tgttaccaga aaaaatggaa caagggttta atcaaaacct ggaagctatt cagcttttac
    21541 agaatgcatt gggaacttcc tttttagaag cctatgtcga aaatgcagaa aatttaatag
    21601 atgattatca agtacgtgtg gtggatggag tgccgacaaa agagacaacg caacggatca
    21661 cagctcttta tgaagagttg aagaaacttt ctttcgagcc agaagaatgg cgcagactgt
    21721 ctcaattact tttattgaag ggcagccaga cagaacattt gcaggcgaac catcaactca
    21781 ctcctgatag tatcggtttt ttatttgttt ttcttattga acaactatac acaaataaaa
    21841 aagcaccggt taagattctg gatattgcgg caggaatggg caacttgtta ttaacagttc
    21901 ttttaaacct atcgaatgcg gggtaccaaa cagaaggaat tggtgtggat attgatgata
    21961 cattgttggc tgtcgcagct tctacaagtg atttgacaca agcaaatgtt caatactttc
    22021 atcaagacgg gttgcaggaa ttattaatag atcctgtcga ttttgcaatc agcgatttgc
    22081 caatcggtta ttatccgaat gacgaaaaag caaaagagtt tttaacaagt accgaagaag
    22141 gccatagtta tgcacatcat ttattgctag agcaatcaat gaaatatgtg aagccggatg
    22201 gttttggttt gtttttgatg ccgagtggat ttttagaaac ggatcaaagt gaagaaatca
    22261 aaaaatggtt taaagaagaa ggttatcttc aaggaatgat tcaattgcca gatgaattgt
    22321 tccgtaataa acaatcgcaa aaaagtattt tgatcttaca gaaaaaagga ccacaagcac
    22381 aacaagtaaa agaagtcttg cttgtgaaac ttgcttcatt aaaagaacca gagaaggtga
    22441 cggaattttt caacgagttt aaaaactgga aatcatctgc tctgtaatca ttcaacgaga
    22501 aggagagtca tatgtctaaa acaattgcaa tcaatgctgg gagttcaagt ttgaaatggc
    22561 aattatatca aatgccagaa gaaaccgtag ttgcgaaagg gatcgtcgaa cgtatcggtt
    22621 tacaagattc tatttttacc atcaagtacg gcgataatca aaaattcgaa caaatcatgg
    22681 atatcgaaaa ccacgaagta gcagtaaaaa tgctgttaga tcagttgatt gaactgcaaa
    22741 ttttaagctc ttatgatgaa atcaccggag tagggcaccg tgttgttcac ggaggagaaa
    22801 cttatgggga ttcagtagtg atcgatgaga aagtaatgga ccgcattgct gaattagcag
    22861 aatttgctcc tttgcataat cctgcaaact taatggggat caaagcgttt aaaaagattt
    22921 tacctgacat tttgagtgtg gctgtattcg atacatcttt ccactcaaca atgccaaagc
    22981 aaaattattt atatagctta ccggtgtctt attatgaaga tttcaaagta agaaaatatg
    23041 gcttccatgg cacaagtcat cgttatgtgt cggaacgtgc ggcaaaatta ttgaacaaac
    23101 cgatcgaaga actaaaaatc atcacttgcc acttaggaaa tggtgtatcg atcacagcag
    23161 tcgatggagg taaatccatg gatacttcta tgggattcac accattagct ggggtgacaa
    23221 tgggtacacg ttcaggtgat atcgatccag ctgttctccc atatttaatg gaaaaattag
    23281 agattgatat tgatgaaatg atcaatgttt tgaacaagaa atctggtctg ctaggcctaa
    23341 caggattgtc tagtgatatg cgtgatcttg aaaagaacta tgaaaaagaa catattcgtt
    23401 tagcttatga cattttcgtg gaccgcatcc gaaaatacat cggcggttat gtgacaacga
    23461 tgaatggtgt tgatgcaatt gtctttactg ctggtatcgg cgagaatgac ggacatgtac
    23521 gtagtgaaat catcaaaggt atgtcttggt ttggttgtga aatcgatcca gcgttgaatg
    23581 aattgcgtgg tgaagaagta gatatctcta caaaagaatc aaaagtaaga gtattagtga
    23641 tcccaacaga tgaggaacta atgattgccc gtgatgtgga gcgtctgcgc ggctaagcca
    23701 aagcattcgt taggataaaa gatgaaacgc atgcaagagg gaattcttgc atgtgttttt
    23761 tttgcggaga agaccgcaaa atggtaattt aacttcaatt tcccttcttt tttacaggat
    23821 cttcttaggg aattttgctt ggaaaagtgg tcaaaaggga aaagactggc attttattta
    23881 ttgattttca ggaaaatccc aatattttat tttcattcat ctttataagg gacattacat
    23941 atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt attcttcgct
    24001 ttatgtttag ctagaaaacc gatccgaaac attcattgat cgaataataa aaacttgatt
    24061 ttccacctgt actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag ttttttgttg
    24121 cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct gctcctgatc
    24181 aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc tgactgctag
    24241 gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat agctgtcgag
    24301 gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga gtttcagcaa
    24361 cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca catcgctttg
    24421 ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag tcaatgtgat
    24481 ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg gcaaataacc
    24541 gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct caccgagtaa
    24601 taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta aatgatctcc
    24661 acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact gctggtgcaa
    24721 gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc tttgcagccg
    24781 atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc gttccagttc
    24841 tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac gaaaacgacg
    24901 aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt ttgtcagctc
    24961 agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat actcaatcaa
    25021 tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt gccgatcatg
    25081 agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc agcgaatact
    25141 gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat cggctgctaa
    25201 aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta accgtgcttc
    25261 ctgacagtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt ctaatacgta
    25321 accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac acttctctaa
    25381 gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt tgaggtcctg
    25441 tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa ctgtaagttc
    25501 ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc tgtcgacacc
    25561 ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga tatcgactaa
    25621 ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg gattcccgcc
    25681 gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg agaccactca
    25741 aaaaggccat ggttatccct ggcctgtgtt atttaaactc agattatcat gagaaaaata
    25801 ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact tgtcccgtca
    25861 acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg gtaattttca
    25921 ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt agttgtcttt
    25981 tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact taatatttat
    26041 cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt ctcgtggacc
    26101 acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg tttgataaaa
    26161 aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt aaacagctct
    26221 tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg caatggcaag
    26281 cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc ttctcgcggt
    26341 acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc atcgatactt
    26401 catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa taagctttgg
    26461 catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt tcactttttt
    26521 tattttactt gaactcattg actgttttcc ctttatttta aacattataa agaccgatga
    26581 gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc tgccgatagt
    26641 tggcaggtcg tctacgtgtt aagctttggt gtggtcttta gctggcaatt ggttttctaa
    26701 atcagttcgg acaacaagca catcgcaagc tgcgtttcta ataacatatt cagaaacaga
    26761 accaataaat aaacgttcta cagcattcaa tcctgtggct cctaacataa tcaaatctac
    26821 ttgattatct tctgggattt ctctggcaat aatttgtttt ggtgagccat attctacgac
    26881 agaggttaca ttattcaagc cagctttctt cgcattgctt tcataatctg ccaatgtttg
    26941 ctttgccatt tcagtagctt gttctgcaag cattccatca aaggatgaaa cggtttggaa
    27001 agaacgtgtg tcaatgacat gtgctaatag aagttctccg ttatttcgga tagctacatt
    27061 gaccgccttt ttaaaagcta attcagcttc atcagaacca tctacggcaa ccatgatttt
    27121 tttgtattgt tgtaacataa gcattcactc ctcaagtcaa tcgtattgta ctcttttatt
    27181 gtagaacact ttagggaaaa agagaattat ttactgcttt ggttcagcaa ttttttttgc
    27241 aaatcggata ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga ccaaggcaat
    27301 aaagcgttga ttgaaaaaag ctgttaaaat acccattacg cctaaaacag catatgcagc
    27361 accgtaaaat tgtaggaatc cttgattttt atcatttttg ttgatcaata caaaaaagat
    27421 atcctgttta ttaaataaat aatagctaac gaataataat actaaagtgg cgataatcat
    27481 taaaatccta atcatgaaca aatcctccaa aataagcaac aaatagaaaa agcaatttgc
    27541 caagagcaaa ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc gttgtttgtc
    27601 ctgtagtatt gatcccgcga attcaagcag gtgggtccca tggtcgtagc ttcgaactac
    27661 caaatgataa ggcatgttac cagcagtgac acaagacggg tcccaagata tcaataaaaa
    27721 tgttcggtca acacacaaag aggacaattc gaacatcaca gatttcccac ctctatagta
    27781 gcatatgaat ttaaaaagag caatgagaga gctcgcagaa acagaaccgt tttcttgtct
    27841 agttttttgg aggatgcttt ttccactctt ttatacggta atactgttga cccagagcct
    27901 gttcatattt ccctgtatct tttggctgat aatactgagc attcttcagt ttatctggta
    27961 aatattgctg attgacccaa gcctgatcaa aatgatgagg atactgataa cccacgccac
    28021 ggttgagtga tttcgcacct ttgtaatggc tatctctcaa atgatcagga acatctccag
    28081 ctttgccctc tcgaatatca gcaagtgctg catctaatgc catgtaggca gaattggatt
    28141 ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc tcaggcaatc
    28201 ccaatttttc agcagctaat acagcgttca ctgtacgagc cgcagcagca ggattaccta
    28261 aacctatatc ttcataaccg ataaccatca gacgccggca aatacttgcc agatcacctg
    28321 cctcaactaa acgggcgaga taatgaagtg cagcatccac atcacttcca cgaatcgatt
    28381 tttgaaaagc cgaaataacg tcataatgag catctccatt tttgtcatgg gtaagagctt
    28441 ttctttggac acattcttca atgatcgaca gtgtaagatg aatccttcct tctttatctg
    28501 aaggagtcga tagtgtagcc agttccagcc cattcaatgc actccgcaaa tcgccgttcg
    28561 ttgcacgcga aaggtgaaga agagcatctt cgtctaactg gattgcttgt tgtccaaggc
    28621 cgcgttcttt atcccttaaa gcatgttcga ctgccaactg gatgtcttgt tctgttaatg
    28681 gtttgacttc aaagatctgt gtacggctgc ggatcgctgg attgatggta atataaggat
    28741 tctcagtagt cgctccgatc aagatgattc ggccactttc taaatgagga agcagaaaat
    28801 cctgttttgt tttatctaat cggtgtactt catccaataa gaggatcact gtaccactca
    28861 tttttgcttc ttccgctaca acttgtagat ccttttttga atctgtagcg gcgtttagca
    28921 tgcgaaatgc ataattagta gagccggcga tcgcacttgc gatactcgtt tttccagtac
    28981 caggaggtcc atatagaatc atggaagaga gcatgcgagc atcgaccatg cggcgtatga
    29041 tttttccttc cccgactaga tgctgctgac caacgacttc atcgatcgtt cgaggtctca
    29101 tgcgataagc caatggctgt tgcataggat tttcctcctt cttgttttta cgtcccttaa
    29161 ttatatcata tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt gttttcatag
    29221 cttatccaac taatattgtg tatgataaag taggtgagaa aaatgacgga taaacaaaag
    29281 atagatgaat ttttttggaa taaaacgacg ccagaagtgg cacgggattt attggggatg
    29341 tatttggagc atgacacacc agaaggaaga ttagctggat atattgtaga cgcggaagca
    29401 tacctaggtc cagaagacga ggccgcccat agctatggtt taagaagaac accgcgtgtg
    29461 cgggcaatgt atgaaaaacc aggtacgatc tacctttaca cgatgcatac tcatcggatt
    29521 ttgaacatca ttactcagcc agaagggatt cctcaaggag taatgatacg tgcaatcgaa
    29581 cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg tccagatatc
    29641 agcaacggtc cgggaaaatt agtggaagca ttagcaattc ctcaagaact ttatggtcag
    29701 tctatagcag atagttcgtt acgcttagtt tttgagaaaa aaaagacacc aaaaaagatc
    29761 aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa gccgttgcgt
    29821 tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat cgagaaagat
    29881 tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta acatatttag atgaaataat
    29941 tgaaaaacaa gtgacagact atgatgtagc tttagattgg gatacaaaaa atcatacgat
    30001 cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg atgatgcaga
    30061 agggatcgtt tcgcaagaac caattatcga atttgaagac ggcgtacttc tttttaatcc
    30121 tcagaaatct gtttttgatg aacgcaatta tctagcagta atcccttatg aaggaaaaaa
    30181 agggttggca aaatcagtag ctgattcttt agtggaatat ctaaatgaag tattagctca
    30241 agggcaaagc gatttgcttg attttttgga tgaagacgat gacgaagctg tattcgagct
    30301 tcactgggat aatcaacaac tagcagaatt agtagaagta aaacagcaaa atgaagcaga
    30361 tacttattta ccgtacccaa gttattaagg agtgacagat atgaaatgga atgaagtaaa
    30421 agtagaaact gaaagtgaag cagtcgaagc agttgctaat attttgatgg aggcaggggc
    30481 gagtggtgtc gcgattgaag attctttaga tgttgaacat tttcagtcgg atcaatatgg
    30541 tgagatttta gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta
    30601 ttttccggaa acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca
    30661 attacctgag tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga
    30721 aagcaactgg gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct
    30781 gacggttgcg ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac
    30841 actagatcct ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac
    30901 agcactagaa acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg
    30961 cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga
    31021 cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt
    31081 ccatgtatca gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc
    31141 gaacatttta gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca
    31201 aacaggcacg ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc
    31261 catgttagaa caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat
    31321 tattttgaaa aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga
    31381 atcaaaagac tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag
    31441 gatggaaccc gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga
    31501 aatcacggag attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca
    31561 aaaggaactg ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga
    31621 ttggatcgtt cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc
    31681 agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa
    31741 gattgctcaa gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt
    31801 ggaaacgaaa caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta
    31861 tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa
    31921 accagatagt cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt
    31981 cgattatttt gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac
    32041 agaaacagca cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc
    32101 ttctttcagc taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata
    32161 gtcaacaatt tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa
    32221 aaagctttta aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac
    32281 aattatcaaa cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga
    32341 ttcctgtgac aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat
    32401 acatgggtcc cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac
    32461 atgacggaca gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag
    32521 gtatattagc agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg
    32581 ttgttgaaga tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg
    32641 ctatgttagt ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc
    32701 agctagcaga aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca
    32761 tggtcaaatt agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata
    32821 agcaaagaag gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag
    32881 ggatcagccg gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac
    32941 agtattaccg tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa
    33001 gtggtactgt tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct
    33061 atggacggcc aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt
    33121 tcaacgaaat ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt
    33181 atgctgtatt aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt
    33241 atattgccat gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg
    33301 caggaaatcc ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg
    33361 gggttgctgc acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta
    33421 tgacgaaaca attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg
    33481 cttctgaatt tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca
    33541 caccaaaagg agacgttact gagttaccaa aaggatccgg accattggac tttgcataca
    33601 gtatccatac agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc
    33661 agcttgatta caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat
    33721 ttggcccaag tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca
    33781 aacgtttctt caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg
    33841 tcaaatgtat tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc
    33901 aagaagcact cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt
    33961 atggagaagt tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac
    34021 aaaaaatcga gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac
    34081 ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac
    34141 taattcgtat cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca
    34201 ctaaaggcag agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac
    34261 caaatgtagc agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag
    34321 agtatgatgc agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac
    34381 ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag
    34441 ataaaatggc gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta
    34501 tcgtggataa aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg
    34561 aggacagata tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag
    34621 caagaaattg gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat
    34681 acgcaaaaag atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat
    34741 gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct
    34801 caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca
    34861 cgaccagaaa cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc
    34921 atcacagtcg ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga
    34981 cctgtaacga tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa
    35041 aactatgaaa cacgtcgctt aagttagact gataaactct aacttaagtg gcttttttgt
    35101 ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa attatacaag
    35161 ttatttttta taggaggttg ctgaagatga aagaagtacc agtagtagat atcaaaatca
    35221 cagatttaaa aaaattgtat caaaaaggat acaacatgtt ggaggagtta cgccatgaag
    35281 cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg atctacggat
    35341 catcagcagc aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt gctatgccaa
    35401 aattagtatt gaagacactt tttggtcaag gtggagtaca gactttggat ggagctgctc
    35461 atcaccatcg gaaaaatatt tttatggatc taatgacacc ggaaagaatg gaagattatc
    35521 atcgtatctt agataagaat ctgacacaag cacttgaagc acagcatggt caatttgaac
    35581 tatttgattt aagtaagatg gttttcttta catctatttg cgagtgggca ggtatcaatt
    35641 tatcagcgat ttctaaagat gaggtcgaaa aattagcaga atatcaaatc tctatgatca
    35701 gcggaacgtt tacttctccg attgatcaca taaaaggtgt cgagaatcgc aaaaaatcag
    35761 aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct ggaaaagaaa
    35821 atgtggcatt atatgctttt gccaatgcca cagatttaga tggtcagctt ttacctctgg
    35881 aagtggcagc tgttgaatta ttgaacatca tccgtccaac ggttgctcta actgtgtggg
    35941 cggcattgat gggacatgca cttttcagta gaccagattt gtatcagcaa ttaaaaaatg
    36001 acttttcgac gttacaagat ccgtttattc aagaaatgag acgctattat cctttcttcc
    36061 caatgctacc tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt ataccagaag
    36121 gcagttgggt tattttagat ttatatggaa cagaccatga tgaacgaaca gttgaagcgc
    36181 cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc tataaagaag
    36241 aatatgagat gattgcacaa ggcgggggaa attttcgaca aatgcatcgc tgtgcaggag
    36301 aatggatcac tcttcatagt ctacgtgtgt tttccgatca gctagtaaat aagtttgagt
    36361 tctctgtacc tgaacaagat tggacgattc cattcaatca gtttccgact tatccgaata
    36421 gccgagcgct actttacaag aattagatta gcataaactt atcgaaaaaa aggaagatca
    36481 aacagtgctg atcttccttt tttcatatta ctataaattg ataaggtcaa ttgatcaaaa
    36541 tccacgaaat gcttcatcaa tggtttgata atcttcattg gataaatgca aatctaaagc
    36601 ttggacattg tcagatactt gctctggacg cttagctcca ggtataacta cagacactcg
    36661 tggattcttc atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt atcttttagc
    36721 aatggggcga agtacatcga cagcggttaa aatttctctg aaacgttctc cttggaaatc
    36781 tggatttctt tttcgaggat ctccttcacc aaattgtttt tcctcaccaa gttcatattt
    36841 tcctgtcagc aaaccggaag caagaggaaa atagggaaca aaactgattt tgtttttttc
    36901 aagataaggg aataattctt tttcagcctg ccgatggatc aaactgtatt tatcttctac
    36961 tacatcgaca tatccatctg cattcgcttc tttcaattgt tctaacgtga agtttgacac
    37021 accgactgct cggatttttc ctgcttcttt taattcgtgc aatgtagcaa ctgattcatt
    37081 ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat cagtttgcag
    37141 acgttttagt gcatcttcca ccgattgttt gagaaactca ggagaattat caaatgttcg
    37201 ccctttattt ggtacatgag cagcttttgt agcgatgacg acacgtgagc gatcatattc
    37261 tttttccttc agtacctcgc caatcagttc ttcggaacgg ccgtttccgt aagcataagc
    37321 ggtatcgatc aaattgatcc cgctattcaa agctgtacga actacttgtt ttcctgtctc
    37381 atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca tttgctccta atccaagctg
    37441 ttctgcgtag acttgtgaat gtccgattct tatttctgcc atagctgatt cctccttttt
    37501 gtctagtcta aaacaaaata ccaaaaagct gaactaaaac gtttttgtga ggggaaatag
    37561 tcagatatct cttgactttg agactggttt ttagtaggtt ataagtaata tcgaatgtca
    37621 atgaaagagc gagtaagttt ttgaaacttt acaagagagg atcatcgagg ctgaaaatga
    37681 tctaaagttt cattagccta aagacactct ggagatggac gagtgaaaag ccgtcccgcg
    37741 tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa attaggtggt accgtgtatg
    37801 aatttcattc acccttgttc atgatgaaca agggtgattt ttttatttac atagctaaga
    37861 aagggagaga aaactatgag tttccaaaga ccaaaaggaa caaatgatat tttgccaggc
    37921 gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt tattattcag agattatcag
    37981 tataatgaaa tgcgtacacc gatttttgaa cactatgaag tgatatcccg aagtgtaggg
    38041 gatacaacgg atatcgtttc aaaagagatg tatgattttt atgacaaagg ggaacgccat
    38101 gtgacgcttc gaccagaagg tacagcacca atcgttcgtt cttttgttga gcataaattg
    38161 ttcggcccag agtatgcgaa accgttcaaa actttttata tgggaccaat gtttcgctac
    38221 gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga agcatttgga
    38281 tcagaaaacc ctgcaactga tgtggagagc atggtgatgg cacttgattt cttcaaacag
    38341 ttggggatca cgcaaatccg gctagtcatc aattcattag gtgataaaga aacgcgtcaa
    38401 gcctatcgtc aagcattgat cgactatctt ttaccttttg aatcagagct gagtgaagac
    38461 tctaaacgcc gccttcatga aaatccatta cgcgtattgg acagtaaaga caaacgagat
    38521 caaaaatttg tggcagaagc tccatctatc ttagattatc taagcgagcc tgctcaaaaa
    38581 cattttgata ccgttgtttc catgctggat gcattggctg ttccttatga aatcgacagt
    38641 aatatggttc gcggactaga ttattacaca catacgattt ttgaaatcat gagtgacgca
    38701 ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac gttatgacaa tctagtggaa
    38761 gaactaggtg gtccttcaac acctggcttc ggatttgcaa tgggtatcga gcgattactc
    38821 ttgactatgg aggcagagga agtagttatc cctgcattta atgagttgga tgcttacgtg
    38881 gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag tggtccaagc gattcgaaac
    38941 tttggttttt cagctgaccg cgacttcatg aaccgtaaag caaaagcgca atttaagaca
    39001 gcagataaag cgaatgccaa attagtattg gtgatcggtg aagatgagct tgctagtcaa
    39061 acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt agaagagata
    39121 tatgaacatt ttgatgaagt ctatgatgaa atgactttat tcgagtagga ggaaaaaaat
    39181 aaatggcaca aagaacgatc tattgcggat tagtttccga acaacaatta gaacaaacag
    39241 ttacattaaa aggatgggta caaaaaagac gagaccttgg gggagtaatc tttatcgacc
    39301 tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac aaagaagcat
    39361 gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga agtgactggg gaagttagaa
    39421 gacgcggaga aaatgcagta aatcctaaaa tggccactgg tgaattcgaa gtgattgcaa
    39481 gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt tcaaattgaa gatgatcaag
    39541 caatcagtga cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt ccaaaaatga
    39601 ctaaaaacat caaattaaga aatgcgacaa caaaagcgat tcgtcattat ttagatgata
    39661 atgactttct cgatatcgaa acaccttacc tagggaaatc tacaccagaa ggcgcaagag
    39721 actacctagt tccttcacgt gtccatgctg gacatttcta tgcgttgccg caatctcctc
    39781 aattgtttaa acaattgctg atgaatgctg gattcgaccg ttattatcaa atcgttcgtt
    39841 gtttccgcga tgaagattta cgtggagacc gtcagcctga atttacgcaa gtagatattg
    39901 aaacaacgtt cttaacagca gaagaaatcc aaacttacac agagggattg attgcgaagg
    39961 taatgaaaga agttcgcggt atcgaagtca cattgccatt tcctcgtatg acctatgatg
    40021 aagcaatggc tcgctacggc agcgacaaac cggatacacg ttttgatatg gaattgatcg
    40081 atctaagcga tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct cttgaaaacg
    40141 gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga tcgctactca cgaaaagaca
    40201 tggatcagtt aggtcaatat gtaggacaat tcggagctaa ggggcttgca tggctgaaag
    40261 tagaagaaga cggattgaaa ggtccaattg ctaaattcat gggagaagca acagaagcaa
    40321 tcattaaagc aacagatgca aaaccaggtg acttgctgat gtttggcgca gataaaagtg
    40381 aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa ttaggactaa
    40441 ttgatgaatc gaaattcaat ttcttatggg taactgattg gccacaattt gaattttcag
    40501 aagaagaagg tcgatatgtc tctgctcacc atccattcac gatgccaaaa gaagaagata
    40561 tcccgttatt ggctgaagac cctgcaaaag tctatgcaga agcttatgat atcgtcttga
    40621 acggttatga attaggcgga ggttctatcc gtattcatac tcgtgagctt caagaaaaag
    40681 tactagaaac tctaggcttt acaaaagaac aaatggaaga acaatttggt ttcttgttaa
    40741 cagctttaga ttacggattc cctccacatg gcggtattgc attaggattg gaccgttttg
    40801 caatgttatt agccggagaa gataatattc gtgaagtcat tgcctttcct aaaaacggaa
    40861 aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa ttgttcgaat
    40921 tgaatattga tgtaacgaat atagaagaat agccgataaa aaggtaaaaa ggatttgtcg
    40981 aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca ctttataatg aagccataac
    41041 atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat catgattaca
    41101 cgaccaagct ttcggtatct atcgtttatg cgttgatcgc atcagtcgca ttgaatttct
    41161 tttatcagcc aggacatatt tattcaagtg gtattactgg tctagctcag attttgacga
    41221 ctttgtcaaa atcactgata ggattcgaag taccggtatc aacaactttg tatttactga
    41281 atataccttt gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca attttcactt
    41341 ttatcacagt cactttgacc tccctttgta tccatatttt accggaaaca gtcctatcta
    41401 ccgatccaat catctgtgcg ttgtttgggg gggctgtgat gggactaggt attgggctag
    41461 cgttgagaaa cggcctatca tcgggcggtc tagatatcgt gagtatcacg attcgtaaaa
    41521 aaacaggaaa gtcgattggt tctatttcga tctacttcaa tgcattgatc gtttttgttg
    41581 ccggaattct atttggctgg caatatatgt tttacagtac gttgtctatt ttcgtcagtg
    41641 gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg atcgtaacga
    41701 aacatccaga aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg atcacgatca
    41761 tcaatgaagc agaaggtgct tataagcatg ataagcagac agtattgatg acgatcgtta
    41821 cacgatacga actgcctcta cttagagaag caatgaaagc ttctgatcca caagcttttg
    41881 taagtattgc agaaaacgtc cagatacttg gtcgtttcta tgaggatggc atttagatcg
    41941 agtttgacaa aaattcatga aatactgctg tagacagacg ttcgtttgtc tgcagttttt
    42001 ttctaccata gtagaatcag caaagaaaaa ctgctttttt atttgaaatt ttcttaaagt
    42061 tgaaaaaaag catgtttatt tttttaaatt tatgggatga tagaaaaaaa tagtagagaa
    42121 aggacacgat tctacttgct attttcactt aatacccgta tattacgaat gaaaaaagtt
    42181 tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt taaaaaacag ccgctacatc
    42241 actgggtttg atgggctccg ttcattagct gtggtgggtg ttatcttcta tcaccttctg
    42301 ccaactagta tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt atctggttat
    42361 ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa tatttggcaa
    42421 ttttatgtca gaagaatgaa gagactctat ccggcacttg tttttatgct tgtgacagct
    42481 tcggcttata tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg agttgtggtc
    42541 agcagcttac tgtatgtcaa caactggtgg caaatcaata acggtttatc ttatttcgat
    42601 cgatttgcta atgagtcacc gtttacgcat atctggtctt tagcagtaga aggtcaaaac
    42661 tatttggttt ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa aagaaaatgg
    42721 atcttttata ctgtgttagg tacatccttc ctctccgcca tcttgatgat gatcttgtat
    42781 acaccaggag gcgatccaac ccgtgtttat tacggtaccg acactcgtct gttttctata
    42841 tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa aaacatcccg
    42901 aaacaagcga aaaatattct gaatatcgct ggaggaatct cgcttttggc actgattctt
    42961 tgcttcttct ttttagatga tcattacaat ttcatctatt acggaggaat gctcctagtg
    43021 agtctgttat gcgtagtgct agttgcagtg acagcacacc ccggagcaag tttgaatcga
    43081 tggctgacaa atccagtatt tacttggatt ggcaaacgaa gctatggcat ttatctgtat
    43141 cagtttccag ttatgatttt ttatgaagca aaggtgactg atattgccga tcatgttttg
    43201 ttgcatactt tagtggaaat cacattgatt ctaatcatca gtgaactttc ttatcgatat
    43261 gtggagcgtc cgctagcacg ttttgattat ggtcgtacat tccaagtgat gaaaggatgg
    43321 ttcagcaaac cgatcatcag taagagcaaa ccatgggttg ttccaggcac attacttgta
    43381 gcaattgcac tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc cgatcaaaaa
    43441 caactgcagg caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag aaacaattca
    43501 gaaaacacca ctagttcggt tgaccaagcg atccttgata agtacgagtt gtccaaggca
    43561 caagggaata aggcacaaaa attagaacta acggcatttg gtgattcagt tatgttggat
    43621 gctgcaacgg atctacaaga actttatcca aaagtagtgg tagatggaga tgtcggacga
    43681 caattatatg ccagtgtacc gtacattgaa aaattaaagg aacaagattt gttgaaagac
    43741 actgttctga ttggactagg aacgaatggc tcattctcgg aagctcagtt tgatagtgtg
    43801 atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc gacgaagcgc
    43861 tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga aaaaatacga caatatgacg
    43921 ctgattgatt ggtacagcta cagtaatgac catagtgact ggttttatga tgatcaagtc
    43981 catccaaatc ctgaagggat gacacattac attcacttag tcagcgaagc cttgctagga
    44041 ggaagttcaa gtacgaacag cagtgagaca aacgtgacag aatgatagct acaaaaaact
    44101 tcaggcaaaa acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt
    44161 catatagatt caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt
    44221 cgggtgagtt ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact
    44281 tctgcatgga tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta
    44341 ccattgttat gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc
    44401 tgatctttac agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat
    44461 ctctgcagtg tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc
    44521 gtctaatttg ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt
    44581 tgggtaaaca aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat
    44641 ttctaagaac cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt
    44701 acttgaaata tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag
    44761 gtgtttatta gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac
    44821 gactaagtca caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg
    44881 gacagtcatt tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag
    44941 acgaggttga agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc
    45001 aactgcgcca tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa
    45061 gtgagcaagc agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt
    45121 gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca
    45181 aaatgaaaaa aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt
    45241 gtcaaacctc tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt
    45301 tttatcgatt tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc
    45361 gctatgagaa taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc
    45421 gtacttggtg ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta
    45481 tggcaaaaga agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg
    45541 cttttccagt taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt
    45601 tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac
    45661 ttaaaggcgg aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat
    45721 atcaaggtcc gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg
    45781 gcgaaccaat cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg
    45841 aacgacgaac acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat
    45901 atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg
    45961 tcctgttcag ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt
    46021 tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa
    46081 taattagaag gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca
    46141 tatcggaaaa aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc
    46201 tatactagat atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata
    46261 tgataggagt gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg
    46321 gaatttaaag gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag
    46381 actttcagcg ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc
    46441 agaagcattt gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga
    46501 accaattgca gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt
    46561 agccaaggtg atagataaaa tgcaggagct atttgattca gaaaaaaaac aagtattgat
    46621 tagccatttt tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa tcgaagtagg
    46681 cgggttggat tcgatccctg gtgaactact tacaccgttt gattatgtag cactaggaca
    46741 tttgcataat caagctgctt tgagacagga taatgctcgc tatagcggtt ccccattgaa
    46801 attctcttta tctgagatca accaaaaaaa aggcgtatgg atcgttgacc tgacaacagg
    46861 cctggagttg aattttaggg agatcgaacc cctgcatgat attgaagaaa taacagcgag
    46921 tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc aatcgggaga attacatcta
    46981 tatctatctc actgatagaa cgatcatccc gaatatgatg aaccagcttc ggcaaatata
    47041 tcctcgtatt ttggggattg aacgggcaaa cggtcgagca gaacaaacaa agcgcaaacg
    47101 aaaagtacta gaaatgaaag atcctcaaaa attagcagaa gcatttttta gtgaaatgac
    47161 ggaagagcag ctaactgcaa accaagaaaa atggctcgga gaaacgttgc aagaaatcat
    47221 ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt ttggtccttt
    47281 tatcaatgag accgtagatt tttcacgttt gacagaagca ccgctttttt tgatcagcgg
    47341 gaagacagga gctggcaaaa cgacgatttt tgatgggatc accttcgcac tttttggtga
    47401 aacttccgga agacttcgct ctggaaaaga gatgcgttct ttgtttgcta caccagacga
    47461 agaaacaagc gtgacttttt cttttgaaca tcagcagatg agctatgaaa tcgaacgaaa
    47521 accagaacaa gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg cagccaaagt
    47581 aacgttgacc atttttgatg ggaaaggaaa agaaataaaa caaatcacaa aacgtacaga
    47641 agcagatcag ttgatcagag aactgatgaa tctcgatgcg aaacagtttt ctcaaatcgt
    47701 tttactaccc caaggagaat tccgtaattt tttggtttca tcaagcagtg ataaagagac
    47761 tgttttacgt catttattcg gtactcaatt tttccaacag ttcaatgagc gattaaaaga
    47821 aaaagcaaaa caacagcaac aaaaattgga tcatttagag caagagctgc atcttctgca
    47881 aaaaaggttt gttttagtca aagaagagga accagcagca gggtttgaag ccgttttagc
    47941 ccaatggaca aaccatcagg caatactcga aaaacaaatc acggaagaaa aagaagctct
    48001 aacggaactt caggaaaaac aaaagcaact agaaaattcc tactatcgtt ttgaaagctt
    48061 gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag caaaaggaat tagctgctca
    48121 aaaagacgaa atagaggaaa agaaacgatg gattgggtat tatgaattta cgcaacgatt
    48181 agtcgaacca atccagcgcc tacaagaaat aaaagttgaa aaaatagaac tgtcacaaaa
    48241 aagtagtgag caaacaaaga aactagcgat gactacgcag aattatgaag agtggcaaaa
    48301 aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc aaaaactgca
    48361 aaacaaacag atgatggtgc caattgtcga atccttacgt agtaaaaaac aagacgcaga
    48421 acaattaaca atttcattgg ataaagaaga attgaatgcc gagaagctgt atcaaaatct
    48481 cgctatctat caggaaagaa ctcgaattct tcaagaaaat ttactggatc aggaaaaact
    48541 gcaagaagat cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc agcaagaaat
    48601 agaaaaacaa aaagcaaaag acactgggtc gctcgcacag ataagagaag aactcaaaaa
    48661 agcaactgaa gaaataacaa aaaatagtca gctattgaaa gaacagacag aacaagcaga
    48721 acagctgaaa agccaatggg caaagcagca aattgctcgt cttcagcttt ttttgcgtcc
    48781 aggggagcca tgtcttgttt gtggatcaac ggaacacccc atacaacaat taaaacacga
    48841 acagccaaat aaggaagata tcttaaaagt agaacaacaa ctggcagaag ctgagtctgc
    48901 tgtagaaaga actcagcaaa tcattgccaa aagtgaagca catgaaaata atcttcaaca
    48961 acaattatca gatttagaac aaaaaatcat ggcaacgaat atacaattgg ctgaacaaga
    49021 agaacacttg caacaggctt tgaaaaaggc atttcctgag ataacagaac aaaaaacaaa
    49081 aacaactctt gtagtaatcg aacaatcttt agatagacga aaaagcaaga tggccaatga
    49141 aagaatagag ctggaagaaa taagacaaca agcagaaaag ctcgaacaac aattagctgt
    49201 ctacaaacag cagcagacag aaaaaaataa tcagctccag caactgcaag gagaaatcaa
    49261 aagtttagaa caacaattgc aagatacgga tagtaactgt ttagaagagg agatacgacg
    49321 acttgaaaaa cagttagaag aagactcgaa tcaactagct gttgagcaga aagcaggagt
    49381 gcaattgttg caagatcttc atacgcttcg gacacagctt gatcttcaac atgaacaaat
    49441 agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa agttagaaag
    49501 ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc aagcacaata
    49561 taaagaaaac caacgtacag ttacttctta tcatgaagag caattagtga cagctgaccg
    49621 gctgcgtcaa ttagaaaata tccagctaac tgagacattc ccagatacag aagagatcag
    49681 ccaacagtta gaagaaataa gacgtatgat cgaaataaag caagaaaaga tatacggttt
    49741 acaagaacaa aaaattagta atcaaaaaat ctacgatgaa tgtctttcga tttatcacac
    49801 aagccagacg aaattagatg agcttacgca attacagcag ctttcgcaaa cattcaacgg
    49861 agaaaatcct aagaagacta gtttggaacg gtatgtgctt caggtttatc ttcaagaagt
    49921 gttacaagta gcaaatgatc atctccaacg tttaacgaaa aatcgttatc aatttgagct
    49981 agcggatacc attggcagtt atcgagggaa aaccggatta gaaatcaata tctatgatga
    50041 tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt ttattgcagc
    50101 actttcttta gcattagctc ttgccgatgt gatccagaca caagctggtg gtgtagcaat
    50161 cgatgcattg tttattgatg aaggatttgg gtcattagat gaagaagctt tggaaatggc
    50221 aatggaagct ttagaaacga tcgaaagtga aggaagaatg atcggcatca ttagtcatgt
    50281 aagagaattg aaagaacgag tattacaaca aataagaatt gatacagaag gtagcggaca
    50341 aagcaaaata cgctatcaac ttggataaaa agggagattg gtaactatga aatggagtat
    50401 tccagaaaaa gtcattgcac gtggacgtca atacttgcaa gaaggccgag tgttatcggt
    50461 tgtccctgac caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg aattgtatat
    50521 ggttgatttg gatggtacag caaaagagat agattactgc caatgtccct attgggagga
    50581 acataaatat tgtaagcata cagttgctgt ggaactttat ttacggtcga aaaatctatc
    50641 tcgtatcatg aaaaaagacc agccaatgct agaaccggta aaagcaacaa gtgagggaga
    50701 aatcattgca aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag cggtgcaacc
    50761 tttgattatt gaataccaag tagaaacgat tgagactaat cagtatcacc cagagctttc
    50821 tattttggca ctttacttgc gtatcggttc attggcatca ccgaaaaaaa catatatcgt
    50881 aaaaaacatc tatgattttt tacaagctta cacagaaaaa gaaacttaca ctgttaataa
    50941 acagtaccag tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa aaatactcac
    51001 gcatcttgca ggaagtgctc agacgcatca attattaggt acgaacggct tacaagtcaa
    51061 aggaaaacta gataaaagat atctgcttct cccagtagaa caagcccgct tcctgttaga
    51121 gcagatgaat cagactacac gtttgcatct gcagataaat gaacaaaacg atcaaggaat
    51181 acaattttct gatcaaaaca aacctttatc attcaaagtg gaaaaatttg gagaagatta
    51241 tttgctgcaa gcattgaatg attttgattt tttctttatg cattatcagt ggggagttat
    51301 ccaagggaca ttttattctc tgacgaaata tcaacaaacg gtttatcaaa cttggaaaca
    51361 attgattcgc cgattggaga aaccagaagt agtgttcaag aaaaaagaat taccgtcttt
    51421 gtttaaagag attatccctc tgctgtcaga gatcggcgac gtagctattg cccctgcagt
    51481 agaagaggaa gtagcagata tccccgtcga atttattttc tttttccgaa aagcaaaagg
    51541 aaaaatccaa gctcgagtcg atttcgtcta tggtgaagtc atctattcga ctgacccgaa
    51601 gcatgaggtc tcgacagaac aggcttttca ggtgttacgt gacacagtag aagagaaacg
    51661 agtacttgat ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt atgaacgtcc
    51721 attgccaagc ggggaagaac tatatgcctt tttccgcaca gaactatctg tcttccgtca
    51781 gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg tatttagatg cgcataaaca
    51841 tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc gatattcgat tcgatgtgac
    51901 agggatagag gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa atgatgcttt
    51961 ttatacatta gaaaatggtc aggttctttc ttttgattca gaagaatttc aacaaacaag
    52021 ccaagtgttg caacaattac gggaatcgat acgtacagaa gaaggaacga tccatgtacc
    52081 taaaaatcaa ggattgatca ttcaaaatca attggaaaaa agcaatgcta cttttagtga
    52141 atcgttccaa acgatggtac aagatctcat tcatccagaa agataccagg cacagcttcc
    52201 caaaggattg aatgcaacga tgagagatta tcaaaagcaa ggttttcgat ggttgaagat
    52261 gttaggtcat tatcagtttg gcggtatttt agccgatgaa atgggacttg ggaaaacact
    52321 acagacaatt gcctttttac tttcggaaaa agaagaacgg aaaagcttct ctgctttgat
    52381 cgtagcgcct gcgagcttaa tttacaactg gcaggcagag gtgagaaaat tcgccccttc
    52441 tttaagtatc caagtaatca atgggaataa aaaagaacgt gaagaattac tggctaaaga
    52501 cacagatatc cgagtgactt cttatgccag cttaagacag gatctagcta attatcagtc
    52561 gcaaaaaatt gattatttga ttcttgatga ggcacaaatg gtcaaaaata gcagtacgaa
    52621 aactgcacaa gcattgagag aattagctgt acctcagcgg tttgcactga gtgggacacc
    52681 aatcgagaat aatttagaag aattgtggtc attgtttgca acgattatgc caggattttt
    52741 cccgactaaa acaaaattca gggaacttgc cgcagaagaa attgcgcaaa tgattcgacc
    52801 gtttatttta cggcgagata aacaaactgt gttgaaagac ttgccggaaa aaacagagat
    52861 gaatctatat tctgcactta cagaagaaca aaaaactgtt tatttagctt acttgcgtca
    52921 aatgcgtgaa gaaatcacat caatggattc tgaagcattc aagaaaaacc ggatcgggat
    52981 acttgctggt ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt ttattgaaga
    53041 ttaccaaggc ggttccggca agctcgaaca agtaaaagat ctactggtag ctgccaaaga
    53101 gaacaaacga agagttttgc tgttttctca atttacaggg atgctgacta tcttgcaaga
    53161 agagttagcc gagctaggga tctcgacatt ttatttaaga ggcagtacga aaccacaaga
    53221 tcggttaagt atggtagacg catttaatgc tggcgaaaaa gatgtttttc tgatttcctt
    53281 aaaggctggg ggaactggat tgaatctcac gggagcagat acggtcatct tgtatgacct
    53341 ttggtggaat cctgccattg aagaacaagc tgctggacgt gcacatcgga tcggacaaaa
    53401 aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg attgaggaac gcatggattc
    53461 actccagcaa gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg aagaacagtt
    53521 gacgaaactg actgaagaag acatccgcat gattttaagt gtaggagaaa tcgaggagta
    53581 agagactcga aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa gcgattgttt
    53641 atttgattta aaaaatagca aggaggctgg gattccattt tatcccagct tccttgctat
    53701 cattttttta tttctatttt taaaggttat acaaagattg tcttcattgt ctctaacagc
    53761 tgatcaaaag aagaaatctc gatatcgcaa attggagtct gttcaatttt gcgttctcga
    53821 tggttgaacc aaagcgcttg ccagttagca ctttttgcac ctagaacatc attgtcgtaa
    53881 ttatcgccaa cgtataaggt ctcttctgga agcatatgaa attctttctc agccagctga
    53941 aaaatctctt tttctggttt ttggaaacct gttgcttgag aaatgagcat attgcgggaa
    54001 ggaatccaat tgttcaattg aagttgcttc agtttttttg tctgatggtc agtcggccca
    54061 tttgtgataa ttcctaatgg gacattcttt tccttcaaga aatcgagggt ttttttgacc
    54121 tcctcatgca agcagatatt atcaagttct tcctcgtaaa ttttttgaaa taagaggcca
    54181 tcttcttccg taatatgagg gtagtctaaa tcttttagtg attggctgat ccgatgtgct
    54241 cgcatatatt ctagtgtcca atctcctgcc attacttttg ggaagttttc atcactatga
    54301 tggcggaacc ggatatatag tggatgcata tctgcatctg aaacaagcgg aacgactctt
    54361 ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac atcaaacaca
    54421 actgatttaa tcaaaatttt acacgccttt cggttttcat tgattttttc acaaatcctt
    54481 gactattgta acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt ttcttactat
    54541 atttctattt ccttgacaga ataaggtttt tatatttcag tttttttcac taaaaatcgt
    54601 gaaacacgtc aataagtttg atgaacaaat taatcattat ggtagaagtc gattgattta
    54661 ttttaatgca atctctgtgg aatttt
//
LOCUS       DAJSBC010000013        54178 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSBC010000013 DAJSBC010000000
VERSION     DAJSBC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.013"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            45..1385
                     /locus_tag="ONT65_000768"
     CDS             45..1385
                     /locus_tag="ONT65_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2328420.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1603..2046
                     /locus_tag="ONT65_000769"
     CDS             1603..2046
                     /locus_tag="ONT65_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR2328421.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2550..3968)
                     /gene="nhaC"
                     /locus_tag="ONT65_000770"
     CDS             complement(2550..3968)
                     /gene="nhaC"
                     /locus_tag="ONT65_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2328422.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4371..5594)
                     /locus_tag="ONT65_000771"
     CDS             complement(4371..5594)
                     /locus_tag="ONT65_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR2328423.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5625..6464)
                     /locus_tag="ONT65_000772"
     CDS             complement(5625..6464)
                     /locus_tag="ONT65_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2328424.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6481..7161)
                     /locus_tag="ONT65_000773"
     CDS             complement(6481..7161)
                     /locus_tag="ONT65_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328425.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7163..8236)
                     /locus_tag="ONT65_000774"
     CDS             complement(7163..8236)
                     /locus_tag="ONT65_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328426.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8292..8530)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8573..8806)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8883..9554)
                     /locus_tag="ONT65_000775"
     CDS             complement(8883..9554)
                     /locus_tag="ONT65_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2328427.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9641..12319)
                     /gene="mgtA"
                     /locus_tag="ONT65_000776"
     CDS             complement(9641..12319)
                     /gene="mgtA"
                     /locus_tag="ONT65_000776"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR2328428.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12978..13415
                     /gene="ndk"
                     /locus_tag="ONT65_000777"
     CDS             12978..13415
                     /gene="ndk"
                     /locus_tag="ONT65_000777"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR2328429.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13437..14303)
                     /locus_tag="ONT65_000778"
     CDS             complement(13437..14303)
                     /locus_tag="ONT65_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2328430.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14463..16556)
                     /locus_tag="ONT65_000779"
     CDS             complement(14463..16556)
                     /locus_tag="ONT65_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2328431.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17305..17928)
                     /locus_tag="ONT65_000780"
     CDS             complement(17305..17928)
                     /locus_tag="ONT65_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328432.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18087..19484)
                     /locus_tag="ONT65_000781"
     CDS             complement(18087..19484)
                     /locus_tag="ONT65_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2328433.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19516..20487)
                     /locus_tag="ONT65_000782"
     CDS             complement(19516..20487)
                     /locus_tag="ONT65_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2328434.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20861..21286
                     /locus_tag="ONT65_000783"
     CDS             20861..21286
                     /locus_tag="ONT65_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328435.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21774..22475)
                     /locus_tag="ONT65_000784"
     CDS             complement(21774..22475)
                     /locus_tag="ONT65_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2328436.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22491..22931)
                     /locus_tag="ONT65_000785"
     CDS             complement(22491..22931)
                     /locus_tag="ONT65_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2328437.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22931..23506)
                     /locus_tag="ONT65_000786"
     CDS             complement(22931..23506)
                     /locus_tag="ONT65_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR2328438.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(24354..24833)
                     /locus_tag="ONT65_000787"
                     /pseudo
     CDS             complement(24354..24833)
                     /locus_tag="ONT65_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(24992..25693)
                     /locus_tag="ONT65_000788"
     CDS             complement(24992..25693)
                     /locus_tag="ONT65_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2328439.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25801..27210)
                     /locus_tag="ONT65_000789"
     CDS             complement(25801..27210)
                     /locus_tag="ONT65_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR2328440.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(27258..29123)
                     /locus_tag="ONT65_000790"
     CDS             complement(27258..29123)
                     /locus_tag="ONT65_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2328441.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29244..30095)
                     /locus_tag="ONT65_000791"
     CDS             complement(29244..30095)
                     /locus_tag="ONT65_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2328442.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30405..31118)
                     /locus_tag="ONT65_000792"
     CDS             complement(30405..31118)
                     /locus_tag="ONT65_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2328443.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31186..31512)
                     /locus_tag="ONT65_000793"
     CDS             complement(31186..31512)
                     /locus_tag="ONT65_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2328444.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31635..31862)
                     /locus_tag="ONT65_000794"
     CDS             complement(31635..31862)
                     /locus_tag="ONT65_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2328445.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32184..33437)
                     /locus_tag="ONT65_000795"
     CDS             complement(32184..33437)
                     /locus_tag="ONT65_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2328446.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33498..34238
                     /locus_tag="ONT65_000796"
     CDS             33498..34238
                     /locus_tag="ONT65_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2328447.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34324..35082)
                     /locus_tag="ONT65_000797"
     CDS             complement(34324..35082)
                     /locus_tag="ONT65_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2328448.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35079..36107)
                     /locus_tag="ONT65_000798"
                     /pseudo
     CDS             complement(35079..36107)
                     /locus_tag="ONT65_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(36127..39294)
                     /locus_tag="ONT65_000799"
     CDS             complement(36127..39294)
                     /locus_tag="ONT65_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328449.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39518..40402)
                     /locus_tag="ONT65_000800"
     CDS             complement(39518..40402)
                     /locus_tag="ONT65_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2328450.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40497..40934)
                     /locus_tag="ONT65_000801"
     CDS             complement(40497..40934)
                     /locus_tag="ONT65_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328451.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(41151..42062)
                     /locus_tag="ONT65_000802"
     CDS             complement(41151..42062)
                     /locus_tag="ONT65_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2328452.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(42519..42947)
                     /locus_tag="ONT65_000803"
     CDS             complement(42519..42947)
                     /locus_tag="ONT65_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328453.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(42975..43613)
                     /locus_tag="ONT65_000804"
     CDS             complement(42975..43613)
                     /locus_tag="ONT65_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328454.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(43617..45599)
                     /locus_tag="ONT65_000805"
     CDS             complement(43617..45599)
                     /locus_tag="ONT65_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR2328455.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            45779..46444
                     /locus_tag="ONT65_000806"
     CDS             45779..46444
                     /locus_tag="ONT65_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328456.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(48379..51528)
                     /locus_tag="ONT65_000807"
     CDS             complement(48379..51528)
                     /locus_tag="ONT65_000807"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HCR2328457.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(51541..52758)
                     /locus_tag="ONT65_000808"
     CDS             complement(51541..52758)
                     /locus_tag="ONT65_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2328458.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(52748..>54178)
                     /locus_tag="ONT65_000809"
     CDS             complement(52748..>54178)
                     /locus_tag="ONT65_000809"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2328459.1"
                     /translation="EYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFN
                     VLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEV
                     IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQK
                     ERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAE
                     EMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSK
                     ADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLF
                     FGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVE
                     KYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLE
                     AISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT        15693 a        11177 c         9063 g        18245 t
ORIGIN      
        1 ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat
       61 tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg
      121 caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg
      181 cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt
      241 taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc
      301 attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg
      361 aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat
      421 ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg
      481 cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt
      541 ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc
      601 aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc
      661 ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag
      721 atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct
      781 tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca
      841 cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata
      901 tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat
      961 cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg
     1021 ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc
     1081 ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc
     1141 tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac
     1201 taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat
     1261 tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc
     1321 tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg
     1381 aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa
     1441 ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc
     1501 ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg
     1561 acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag
     1621 tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc
     1681 tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt
     1741 gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata
     1801 aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga
     1861 agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc
     1921 gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa
     1981 gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg
     2041 gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag
     2101 aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg
     2161 catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc
     2221 ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt
     2281 ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg
     2341 agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc
     2401 ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt
     2461 ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa
     2521 aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact
     2581 gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc
     2641 gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata
     2701 ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta gttgattact
     2761 gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa
     2821 gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc aataaaaata
     2881 tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt
     2941 cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat
     3001 gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta
     3061 agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca
     3121 gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg
     3181 atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa
     3241 aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact
     3301 tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag
     3361 aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa
     3421 tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct
     3481 ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt
     3541 gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca
     3601 gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc
     3661 aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc
     3721 aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg
     3781 attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt
     3841 ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca
     3901 atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt
     3961 tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc
     4021 ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt
     4081 taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac
     4141 tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg
     4201 gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg
     4261 acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc
     4321 atttttctga aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta
     4381 gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat
     4441 agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca
     4501 atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca
     4561 atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt
     4621 cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca
     4681 cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca
     4741 tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct
     4801 cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt
     4861 aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta
     4921 atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta
     4981 aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg
     5041 gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc
     5101 ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct
     5161 cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat
     5221 acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg
     5281 gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat
     5341 tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct
     5401 gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc
     5461 aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga
     5521 gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag
     5581 attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat
     5641 gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct
     5701 tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt
     5761 gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca
     5821 tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct
     5881 acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt
     5941 ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc
     6001 aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt
     6061 gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc
     6121 agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa
     6181 ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc
     6241 acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc
     6301 aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg
     6361 atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata
     6421 attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt
     6481 tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat
     6541 tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga
     6601 tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat
     6661 cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata
     6721 cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc
     6781 aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa
     6841 aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt
     6901 caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt
     6961 atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa
     7021 cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata
     7081 tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata
     7141 tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta
     7201 aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt
     7261 ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt
     7321 gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat
     7381 gagcgattag aggttcgttc gtctgatttc caatataact taattccacc agccatttat
     7441 tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat
     7501 gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga
     7561 tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt
     7621 ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca
     7681 atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt
     7741 catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt
     7801 tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt
     7861 ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag
     7921 aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca
     7981 taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct
     8041 gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg
     8101 tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta
     8161 aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat
     8221 ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa
     8281 aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc
     8341 acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat
     8401 cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt
     8461 gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca
     8521 atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga
     8581 cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa
     8641 aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac
     8701 aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc
     8761 ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg
     8821 gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat
     8881 atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc
     8941 gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca
     9001 ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct
     9061 tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga
     9121 atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca
     9181 gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg
     9241 cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag
     9301 tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc
     9361 tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt
     9421 tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc
     9481 caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat
     9541 attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac
     9601 tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc
     9661 gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa
     9721 ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg
     9781 taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg
     9841 ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt
     9901 gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct
     9961 gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca
    10021 tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt
    10081 ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat
    10141 cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag
    10201 gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa
    10261 aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag
    10321 aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt
    10381 ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact ttcattgcag
    10441 gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg
    10501 tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta
    10561 cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga
    10621 tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa
    10681 cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg
    10741 gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa
    10801 attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt
    10861 cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa
    10921 cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg
    10981 tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa
    11041 aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg
    11101 ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat
    11161 ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt
    11221 caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg
    11281 caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac
    11341 ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa
    11401 ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga
    11461 tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat
    11521 ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac
    11581 caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat
    11641 tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata
    11701 ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata
    11761 aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa
    11821 caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta
    11881 cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg
    11941 actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga
    12001 aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa ataattccta
    12061 atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg
    12121 gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt
    12181 gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt
    12241 ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc
    12301 ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg
    12361 atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta
    12421 gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt
    12481 taattcatga cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt
    12541 ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga
    12601 aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact
    12661 gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca
    12721 attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata
    12781 ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga
    12841 ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc
    12901 ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta
    12961 ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac
    13021 gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa
    13081 tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc
    13141 gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc
    13201 tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg
    13261 atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc
    13321 acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg
    13381 atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag
    13441 ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct
    13501 aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa
    13561 gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt
    13621 ctttctttat gtgctaagct ccgtttcata tcttcattca cactacgtgc ctttttcaac
    13681 cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca
    13741 tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca
    13801 atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca
    13861 tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca
    13921 aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac
    13981 agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag
    14041 ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc
    14101 agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat
    14161 ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc
    14221 ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc
    14281 ttgatcaatt caccatcata cataatagtt ctccttttta atttaaattt ttccacattc
    14341 aataaataga tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca
    14401 taaaaaatac caaccttcaa gcattagaat gttgtctaac tactgaaggt tggtttgatt
    14461 gtttattgat tatttttttg ctgttcaacg tctgttcttg ttactggata taagttggtt
    14521 gttgttttat taccattttt tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg
    14581 attgcttcat tacgttccgc ttcattttta tagtcatatt ttttcggatc aatcgtctca
    14641 aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt
    14701 aacgcagtct tgactttttc ctgatctttg gagatttcct catcgattcc tgctgctttc
    14761 gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg
    14821 ggtgtcacaa agttcccatt tcgaaaagca actacttgat catgctgaga agataataag
    14881 tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc
    14941 aaaacatcga tttctccacc gtattcatga ttgactgtcc ctttagtata accaggcata
    15001 tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga
    15061 tctttaccaa gagcgcttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc
    15121 ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat
    15181 tgttccaatg attgatccag ataatgtgcc gttcgaacat aattatctac tgtgctattg
    15241 ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg
    15301 gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gctcaagata ttgggcagat
    15361 tctctgaata aatctttatc taatatccca taacctaacg tttcgtcgga ttcatcaaaa
    15421 taagatcgat caaagaaatt gtcataacct aagtttttat atacatgatc tctattccaa
    15481 aaactagcta cattcccatg gaaaacagcg cttgtatagc cttgcttttg tccaaggatc
    15541 gctggtgcgg cttgaaagac attgtctgac ccaagttctg tgaataatga gccttgaggt
    15601 aaaccaaatg tgccagtttc caacatattt tcagcatcac ttgtctttcc ctgtcctact
    15661 tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct ggaaaatact atttaaaaac
    15721 ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa actgctggaa actttctaaa
    15781 tggagaacga tcacattttt ccctttagcg atcccaaatg tttcgggatt tggctctgca
    15841 taatgtttct ttgtataatc taaaacttca tcaatcccat tactgcttgc atgcgcgcga
    15901 acactgctag tcattcctgt tttgattccg tcataaatag tatacgcatc gattcctaga
    15961 tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca attgtggacg gttggcttcg
    16021 ctcagcgcta gatttcctga aaaaataagc cctgaaagac aggtaacagc gatagccatc
    16081 ggtttgccga cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc
    16141 ccaatgaaca cagcgatatc cagccagtag atgacatcat ggggcttcat caaagaaaag
    16201 gagcttcctg acaatccttg cgacactttc gaaaagccta atacagattt gactgtaatg
    16261 aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata
    16321 tcaatgatca aaagaatagc taacgctggc tttggctttt taatatacaa agaaaggcca
    16381 aaaaacaata aggtagtcgc aattgggttg atccacaaga taaagtactg gatcgtacct
    16441 tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa
    16501 acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat
    16561 atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa
    16621 aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccgtc cacttttcac
    16681 agcataaaac cgctgcgatg taagcgatta aactaatgaa gagataaaag tcttgtggtg
    16741 accgcttcac tccacggtat tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc
    16801 tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc aggacagctt tttcacaact
    16861 tcttccttaa acggtgttct cctgccaact cctcggccac aagtcacaat atttataaaa
    16921 catgagaagc tgttttttga aattgttcct tgtggctcag agttaaacgg caggctcaag
    16981 acctcttgat acacggtgtt caatcagctg accctcggta ttagattatg tcaggacgct
    17041 aattttacaa cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    17101 ccaaacgaaa aatataagga gctatttttt gaaattgctc cttgtggctc agagttaaac
    17161 ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccttcgg tattagctca
    17221 aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaaa
    17281 cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc cttgtgatga
    17341 ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt gtccactgcc
    17401 agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag ttgttttctt
    17461 gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt ttttgtctac
    17521 tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt tgtcgccatc
    17581 atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc tgagagaaat
    17641 cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt ttggcaaata
    17701 aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc accaataagt
    17761 tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc
    17821 tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa
    17881 gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc tttcctccac
    17941 tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt
    18001 tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat ggagaatttc
    18061 acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc tgatacgtct
    18121 ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa gtctactgaa
    18181 ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat acatgtccac
    18241 atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata tttcagatga
    18301 gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt gataaatcgt
    18361 gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt tccataatga
    18421 tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca tccgcgataa
    18481 ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg cattggaccg
    18541 tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta ataattgata
    18601 cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg gatcacaggc
    18661 atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc ttttacagct
    18721 ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt aacatcttca
    18781 gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc aataacacca
    18841 ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc ttcatgatag
    18901 ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg atataaataa
    18961 cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac tttcgaacca
    19021 aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc tcggttcaac
    19081 catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt cacaaagggt
    19141 tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg
    19201 ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa
    19261 ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata aacttgcgag
    19321 gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta ccaataatca
    19381 aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg gccacttgct
    19441 gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc acctcttttt
    19501 cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa tcactttttc
    19561 attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc gttccccgat
    19621 tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt ttttatctaa
    19681 ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt gattgaaaca
    19741 ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg cccctttttc
    19801 taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg taaagacctc
    19861 taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac gttctcttgc
    19921 tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt gttcaccatg
    19981 atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat caatacctgc
    20041 tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga gcatttcaaa
    20101 tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat gcttcatcag
    20161 gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta ataataagct
    20221 atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc caaaacggct
    20281 ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca accctttagc
    20341 aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt tcatgttctt
    20401 ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa caccagccat
    20461 cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac atttgatttc
    20521 tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct tgtcatcaat
    20581 atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt atacttaaac
    20641 aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc aacacttttt
    20701 taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt ctaaaattta
    20761 ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt ttacattcca
    20821 atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat taatcgtact
    20881 atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg gcggctctga
    20941 ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag cagataccac
    21001 caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg gacagttgac
    21061 atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa ctgattcttc
    21121 tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca aagatgacga
    21181 atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta caaaaatcgg
    21241 taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc gtctgttgga
    21301 tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg attgtaaagg
    21361 agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac aaaaatatcc
    21421 atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac aaactagtca
    21481 aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa
    21541 gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca aactcagctt
    21601 cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt ttacaaatca
    21661 aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac gatagaaagg
    21721 cttattctac atcacgtagt aatttttctt tcccgtcgtt tggtttttta tttttagcct
    21781 aattcttgac cttttgcatg ctggttatcc gtaaaatatt ttccagcagg ctttccttcc
    21841 aattcagcta atttttcttg gatctgacgg atcacctttt ttgcatcttc tggattttct
    21901 acaaaatcga atttatcccc atccacttgt attttaggac atacatcaaa atcttcatac
    21961 cattgattat agcgggtgtt caacgtttca taataggtat ataacgtctc atcgctttct
    22021 agctgttcat agtcacgccc acgttttttg atacgttcta gcatcgtgtc caaagaaact
    22081 ttgatatgga ccatcaaatc aggccgtttt tttggagcaa catcatctaa ctcgtttagc
    22141 atcgtatcta ataagctatc gtactgctgt acttcgatat ccgttacccg acctaaatca
    22201 gcgtttagat ggaacaacag gctatcttca tagatggatc ggtctagcac attgtcatcg
    22261 ttcactaaag catttttcat tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa
    22321 aaagcgtact gctcaggatt tgaataaaag agcggcaata cttcgttatc ttcgacattt
    22381 tcataaaatg gtttagaatc aatctctttt gcaagcatat ccgtcaaact gctcttacct
    22441 gcaccaatcg ttcctgaaat caaaatcact gacatacaac ccttcctctt ctataagtac
    22501 tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat attcacgtac agcttggtct
    22561 gattttaaat acgtatggag actttgtgta atcatcaagt tcgtaggtac cgtctcttct
    22621 tgtacaatga tcatcggttt tttcaacata gttgccacac ctaattcgta agccgttcct
    22681 ggatcgattg tttgatgctc aaagtcaagg atagcaacaa caaattctgc atttgtcagt
    22741 tcttccatat ctttttcgta tacttcctgc gcccatccag cgctgaatag ttcataattg
    22801 ctttcttggt gatgacgtgg agagtagaaa cttgtgactg ttttgttttc ctccaacgct
    22861 ttttcgatac gacttactcg atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat
    22921 atttgactca ttattttgcc tccttcaata ataactgatt tttaatagat actgctcgta
    22981 attcattgac cagttccaat ggattgttgg aaaagattac ttgatccaac cattttttat
    23041 ccataaaatc ctcttttgca aaagtttcta ataattgttg caagccgctg taataaccgc
    23101 caatattcaa tattccgatt ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa
    23161 gttgtgagaa ttcctccaat gtacctagac ctccagggaa aacgaggaca gcatctccct
    23221 tttcaaataa taataccttt ctttcatcga tcgtttctgt aggaataaat gtagtaacat
    23281 cttttccagg caattcagtt tcaaaaagcc cattcggata gatacctgtg acttttccgt
    23341 tattttccag tactgtttta ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa
    23401 ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac
    23461 tttctttatt accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa aaacactccc
    23521 taactcaaaa gtctatcttc attctacacg tgttttttta aaataaaaga ctagattttg
    23581 tagtttttta gtggtatata ggatagacgc cactatatat agtatttaga aaaaaatcat
    23641 ttttcagcca ttttatagat aaaacaaaaa taaataacgt gttttcatcg atatgaaatg
    23701 acttacttat agaagatact gggatttcgt cctatatgcc aataacgtgt attattttaa
    23761 acaacaaata cttgtttctt ttattaacga aaaaatcatt ttgttctcgt gttatgtttc
    23821 actgataaat gatgtttctc taaataaaaa agccgacgaa ctaaacgatc aaaatttgat
    23881 catttttgtt catcagcttc tcctaaaaat ctttttgtta acgataaata gctggatttg
    23941 taaaaatatc acttgaatcg tcactgctgg aagaaaattc agtgataacc gcacctgttg
    24001 cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt gtactgatct ctagcatgaa
    24061 gaataatttc tttttttact gtataataac tttctttcct ttttggaata gtacaggaaa
    24121 gtttttttgt ttcctttcct tcgacataag gtataccaga ccctttcgac aacgaaaggt
    24181 tctcattttt ccttcatcgt catttcaagg agggtgccga tgctctggac agtttcgatc
    24241 gctcctaaac caaaatctgt atattcgatt ctttctaact ctgattcttt cataccattc
    24301 ctgtctcaag gtacatctat tgtacttttt tcttgtaatc cattctgcct ctcctaacta
    24361 attgtcaaat catcccaaga tatgtcttct tcctcttttt cttcttggat gatttctgca
    24421 ttcggatcta ctggtttttt caataataaa atggttattc ctgtgacaaa agaaccagct
    24481 aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa cattcccgat
    24541 gccggaatac ggctatctgc gccccaaaac atgcttaatc cgccacctac tgtcccaccg
    24601 actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa acatccctct
    24661 gtgatctgac agatccccat aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg
    24721 agtatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc gaatggggtt
    24781 accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga aaatcgtgcc
    24841 acaccccctt atccagaata aatggtaatc cagaaacagc cgtgtatctg cggtaataag
    24901 cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatgc
    24961 agggcagctt tttcacaacc tcttttttta tttatgtttc ataatcaata tggtaaggat
    25021 gcagctccgt atggtagacc gcctccgaat attcaatcat ttccccaatt tgcgtagacg
    25081 atttccgtat ccgtttgatg ccgatctgtg cgtctgtttt cagaattcct tgttcttctt
    25141 ctgttaaaaa tactgccgag aaatcatcag aaaatgagtc aatagtatat ccttttttcg
    25201 tcagcaaacg atacaaagat tctttgccaa attcttctct gctgaattgc atcatttctt
    25261 gtggaagata atgaacaaaa taaatataag gctgattgtt caaataatat aagcgcttaa
    25321 aacaaacagc ttgctcaccg aaaaaggaaa acaaaggatc ttccttcttt atatggacca
    25381 attgcaaatc cagcgtttct ttccggactt catatcggga gttctccaag atttgcgtaa
    25441 agcttgccgc tttggataat cgattgtaag gacgattgct tagaaccgtc gtcccacgac
    25501 cacttttctt tgcgacatat tcatctgatg ccagcagttc gattgccttt cggacagtga
    25561 ttttgctgac ttgaaacagt tcttctaatt cactttctgt cggcaacatc gaaccgactg
    25621 gatattttcc tgagaaaata tcttctttaa ttttatctgc gacttccaag tatagtacac
    25681 gtgtcctttt cataacgtca ccctttgctt tactattact aaaagaatag cacataaaaa
    25741 ataaaaaaag aacacaaatc acatgtgaga agtgtgttct ttttcattca gctgctttaa
    25801 ttacaagttt acgccatttg aggcaattac ttctttatac caatcaaaag attttttctt
    25861 tgatcgattc agtgtcccat ttccttcatc gtctagatcc acatagataa agccgtaacg
    25921 tttactcatt tctcctgttg atgcactgac aagatcgata catccccaag gcgtatagcc
    25981 catcaaatct acaccgtctg ctactgcatt agccatcgct tcgatatgac gacgtaaata
    26041 atcgatccga taatcatcaa taatcgtgcc atcctcttcc atatgatcaa tggctccaag
    26101 accgttttca acaatgaata atggtttctg gtaacgattg tataattcat tcaatgcgat
    26161 tctcagccct tctggatcga tctgccagcc ccattcactt gcctcaagaa acggattttt
    26221 tactcctccc agcaagttcc cttctgaagt tgctgcttgt ggatcagtct cattgattgc
    26281 cgtagacata taataactaa atccgatgta gtccactgga tattctttta gcaactggaa
    26341 gtctttttcc gtttgctcta atttttccgg atcgactcca tattttttat gggtacgcgc
    26401 tgtgtatgca gggtattctc cacgtacttg gacatccgtg cagaaaaaat tgaattcttg
    26461 gttttggatc attgttgctg cctgattgac tggattggca tcgatgctgt aagttgttgc
    26521 atagataatc atgcagccga tttggatatc cgaacgcaat tcgtggccga tttttacggc
    26581 tttgctactt gcaacgaatt gattatgcca tgcttggaaa atattttggt actctcctgc
    26641 tccattgctt tttactaatc cttgacttaa agctggaaag tgaaaggccg aattgatttc
    26701 attgaaagtc atccaatatt tgactttact gctgtatctc tctaatactg tctgagcaaa
    26761 tcgttcatag aagtcgatca gttttcgatt tttccagcca ccgtattctt ttgccaaatg
    26821 aagaggcatt tcgtaatgag aaatcgtgat cacaggttca atatcatatt ttaagcactc
    26881 atcaatcagc tgatcataaa attctaatcc cgcttcgtta ggagtacctt cgtctccatt
    26941 tggaaaaatt cgtgtccagg caatcgaaaa acgataacat ttgaatccca tttccgcaaa
    27001 caaggcaaga tcttctttga aacgatcgta atgatcgatt cctcgatgat tcgggtaaat
    27061 atatttttct tgatcaatcg tccagtcaaa ttcttcactg ccgataatcg caaagcgctg
    27121 cttacctcca ggcatagcat ctgcaacgga tagtcctttt ccgtctcgat cataagcccc
    27181 ttctaattga tttggcagca gttgctccac cccataaaaa tttttctgaa aaacgtgatt
    27241 gcatcctgat ttcctcctta ttttacaatt gccagaacat cttcgtttgc aataatctct
    27301 ttttggtcta attcaagtat atcggcaaat gcatcagtat tcgtaatgac aactggagtg
    27361 attacgctat agccagctgc tctaattgct tcgatgtcaa aacgaatcaa taaatctcct
    27421 gctttgacga gatccccttg tttgactaag atctcaaagc cattcccatt taattctact
    27481 gtatccatcc cgatatgaat caaaatctcc gctccgtcag tcgttgtgat ccctacagca
    27541 tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag ttctcctact
    27601 tctggatcaa tagcgatacc ttttcccatc acgccagaag aaaaaacttg gtctggtact
    27661 tcactcaatg gcagtacttt acccgttaaa ggacttttta aggtatgccg tgctgaagta
    27721 atcggttttc ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca
    27781 aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag caattcctgt agctattact
    27841 gctactgtca catttgactc tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat
    27901 aagctgacca aactgctgga gaaagctttt actccggaaa atccgataat agctccacca
    27961 atacctccag aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt cacaccatac
    28021 acagttggtt cagtgattcc aaataacgaa gtcactgtag aagaaatccc taatgctcga
    28081 agtttcgtat cttttgttcg caatgctaca gctaaagcag ccccaccttg tgccaagatg
    28141 gctggaagaa gcattggcat caatacatca aacccatatt gttcgatatt caaaaacatg
    28201 atcggtacga atccccaatg catgccgaac atcacgaata cttgccataa acttcccatg
    28261 attagacctg ctacgattgg actaaatcca taaatcgagt tgaagagacc gcctaaagca
    28321 ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac catcactact
    28381 aaaacgacaa gcggaactaa aatcatttgc agaaattgcg gaatcacttt ttttacgaat
    28441 ggctctaatt tactttgaac ccatacggct agaatgattg gtatgacaga agaagtataa
    28501 ccagatggtg aaagaatcac tgggatccca aagaaactga tagcttctcc cgcaccagca
    28561 actgctgtga tagctggata cacaagtgcc atagcaattg ctactgctaa aaactggttg
    28621 gctttgaact ttttagctgc tgtaaaagca agcatgacag gtaagaaggt aaagacacca
    28681 tctgctgcag caaacaatat ttgataagta ccgctatcag ctgtcagcca gcccatcacc
    28741 gttgccaatg ataagaatcc tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt
    28801 gtaaatatac tagagataat atcgatcaga cgattcaaaa tatttttgga ctcttctttt
    28861 ccatcctccg aaactgcaag accttcctca tccacgatcg attgataaac atctgctaca
    28921 ttactgccaa tcactacttg atactgaccg ccactttgta cgacttggat cacgtccggg
    28981 tcttctttta acttttgcgt atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc
    29041 gtagcacaat gtactaaact tttgacattc ttttgtcctc ctaccgcttc ccatacacga
    29101 cgaccaactg cttgattatc cattgttttt tccttctttc ttttttattt aaaaaataaa
    29161 aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca atccaggttt
    29221 tgcctgccat acagtaacaa tccttatgat tggatgcttt ttttctcact tactactctt
    29281 tgaatatgaa tcgttaaata aatctgctct gcgatcccca tttcctgttt ttgcgtttgt
    29341 cgaagatact cattgatccg ttcgacacaa tgaaacgctt ttggatactt tccttgaact
    29401 aatgcgtaga gaaattcatc ttgctcgtca gatccttccc ctttgagata acgctgggca
    29461 aaaaactgca gatgagtaat gatacgctga taattcaaag attcttcatc gaacacgatg
    29521 ccgaagaatt tactgatgat actcagtata tctcttacca ttgtggtagc ggtcatcgtc
    29581 acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct
    29641 tcatcctcag aaagttccac acccgtcttt tgtttcacga acaaaagagc ctctttcgct
    29701 gcttgatatt ctttcggata aaattttttt gtttcaaata ataatggatt aggcagttgg
    29761 atattttctt ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt cagataaata
    29821 ttaggcgaaa attcaacttt tagtttatct tcagccagtt cgactacttg tttgacccat
    29881 tcgatctcct ctgccggaat atcagccatc agctgctgga attggttttt agcaaaagaa
    29941 tctcttaaaa caaattcttt ttggaccgct tctggcgaaa tctcgtcccc aatctttttt
    30001 tgaaaagcta gtccttttcc catacaaacg acttcttccc cgaatccatt tttcgaaatg
    30061 acgacattgt tattcaaaat tttatcaatt ttcattttgc ttcgccctct caagaaaaat
    30121 aaaaaaacct agaattcgcc tactgtttct tagctgaatt ctggttttgc ctgccgtaca
    30181 gtaacaatcc atttcttatt caaaatcatc ataccatcga atttttattt atgcaagcct
    30241 tttcatataa aaaataaaaa aactggaaca caagtctatc tagaaaacaa ccaaatcaga
    30301 gatgcacctc tttgcgtttc tctttttttc agcaaattgg tttttcctta tcgattcata
    30361 tcctttggct gtttttaatc tgtaacgact tatgtcccag tatcctaaaa tgtttttgtc
    30421 tctagattga aagcagatct catatcatct gtcgcatctt ttatcgatgt aacactttcc
    30481 agttctcctt gtacaactag tcgcgtgacg gcgtccatgt cgccacaagt aattagtgtg
    30541 acaatttctt ttccctcgac ttcattcagt aattgggtat ctgtaggagc aactttttct
    30601 ttaaaaaatg ttttgtatgt atacacattt tctaaatctg tcaaatagat cttggcaccc
    30661 atctctaaat tttctagcgg tgtaaacagc aattcagggt tcgttgcacg atggcttgct
    30721 aaggcataat ttccttttcc catttcttga tctggagata atgttcctgc tccgtaaagt
    30781 agtgacgtat tatccaagcc tttgaaaacc ggtaaattga ttgaaactga agggactgcg
    30841 atactagcaa tcactggtag atctgttcct ctcatttgcg acctcatgac cccttctgtc
    30901 gtcattggtt ctaccgcatc aaagtcaaaa ctgacatctt tatcgttatt ctttttcaga
    30961 tcttcttttg ttacattcgc tatagcatat ttatctcctg tctctctgac aaaataatcc
    31021 ttgatctgtt cattgaaaat caaagctagt cctactaata acaagaggaa caagaggata
    31081 ttgactagcc atctttttgc ttttcctttt tttttcattt tctctccctc cagactcatt
    31141 ttactgtttt tcttttgctc tttccaacag ccgaatgaca catttctaca actctgtgta
    31201 tttttttcgt acacctgcgc ttttttctac aggatatttc aaattatttt tgatcaattt
    31261 ttcttcaatg atctcatcta gatccaggtg aaggttgtct gccatcatgt atgaatagat
    31321 caatacatct gccagttctt ctttgattcg ttctaattgt gtttctttta cttcttccgg
    31381 cgttttccat tgaaaaagtt ctaacaattc agatgcttcc aaagaaatag agatagccaa
    31441 atctttttcg ttatgaaatt gtcgccaatt acgttcatct ctaaactggt tgactttttc
    31501 catactattc atttgcttgc cttcttccaa tcacttctta cttgattaat cataccattt
    31561 ttttacataa aaaatagatg atgcttttaa aaaaactcct gttttctttt atttaaaata
    31621 aatgtcatag aaaatcaata atttaggtag ccaccatgtg tatgcagtag cgtgtcttct
    31681 tcccttgagg ctttttgttt ttcgatacgt tcttcttccc aaacagacaa cgctactaga
    31741 atctctctta atgattttcc ttcttcggtg agcgagtact ctactttagg cgggacttgg
    31801 ttataggctt ttcgatggat actttcatct tgttctaact ctcttaactg ctgggtcagc
    31861 attttttttg tgatcgtagg gatttgcttg cttctccacg cctaaaataa gcgatattgc
    31921 tcttcccttt atctggtgta agcaaagcag ttagcatttt gacgatcgtg ggttccgctt
    31981 ttcacttctt atctacttag tatcatgaag tatactttcg tactaatact aagtagcgga
    32041 agtgtcaact actttgttta actacatagc aaaagaaaca gaagggtgac aacggtcatc
    32101 acgcttctgt ttcttttgaa tatgctgtgt tgttgtctct atctccgtgt agactttcct
    32161 caaacttatc ctttgacagt aagtcagtag gctgatttgc tctttctaga tgccagatac
    32221 atttctacaa aaaaatagtg attctgtttt gaaaattcga tttttagttc tgcattttcg
    32281 taaacatcca acattttttt cgcactgtat agacctaacc cgccattttt tttagaggtt
    32341 tttcctcttt tgaccatctc cgatagcgtc aaattcgttg gatttgcctt gttcgctata
    32401 ctaaaacgtg atccctcttt ttcttccagc agattgatcg taatcggact ctgatcggct
    32461 acacgatgat cgaatgcgtt agaaataaaa atagataaac agcgaataaa aacaaccaga
    32521 tcaatcggga ttgttttcac ttcgacagga ataactactt ggatcgggat tttctccgat
    32581 tctgcttttt ctagaaattt taaaaagaca cccctgacag ctggggatac aatatttgtt
    32641 agctgttgta tttgcggttt ttcaaaaata gctgcagaat gatcttctaa tatttctaaa
    32701 aattccaacg tttctttttt attttcctga agagtaattt ttaatgaaaa cagtagactt
    32761 ttataatcat gtttgaattc ctctagatga tggtaatatt cttcatttat ttgatacagt
    32821 tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat aatggaaaaa
    32881 aacaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata attagatgag
    32941 cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa aatacaaata
    33001 cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct cgtctgtttg
    33061 tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa aaaacttgct
    33121 gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa aaagctgagt
    33181 ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt tttcgtagta
    33241 aaataaaaaa gaaacatcaa taataagcca actaggtaag gaaagctcac tttagggata
    33301 ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt tactagcagt
    33361 tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt caaataaagt
    33421 gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac acatattttt
    33481 ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag tttcacagcg
    33541 aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag ctcacttcta
    33601 tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat gttttcttta
    33661 tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa atacgtaaaa
    33721 aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca gtagagatca
    33781 tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta tctgaacaaa
    33841 ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat gtcgataaaa
    33901 tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt ttatatattg
    33961 aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac aactacagaa gataaattag
    34021 tgaatacttc tttaaaacaa ttgaagacag agttagaaaa gtatgcttac tttttacttt
    34081 taaaatctta ctgtatcaac ttatcgcaat tgcagaaaat tgattctgct cattatgtgt
    34141 tagaattttt caacggtgac tccttactcg ttggtcgaaa gatctttgaa aagacaaagg
    34201 aacgtttcca tgacttccaa aaaactgctt cgagttgatg aataagagct gagacgtaag
    34261 catagcagat ttcagggagt gggccttgaa tcggctccac tccctgaaat cgttgatatg
    34321 attttacgct tcctcttctg taggagcatc tgttaacgac catgtgaatg ttgaaacgta
    34381 gttgtctaca gcataattgg atacaggaac aaataattcc actgattgtc tcccttgttc
    34441 cacattctct cctaatgaat aaatccatgt gccaatccct tcatttgctc ctgcagttac
    34501 tagatctact gcctgatctg ctgggatttc gattccttca gcgccagttc cagaaacggt
    34561 agatggcatt ccactgctat tagttgcgct atttcgaatc acggcgtttt tgaatttcac
    34621 ctttgaacca gttagtttat tcgtatcaga tgttgtacca ataaattctg ttcgtttgac
    34681 tgataatttc caaccggcat aatttccgct tttatccgtt acttgtacga aattctgtac
    34741 ttgtgtttgt gctccagtac tgtccgttcc gctttgtaat gcagcatata ctgtttttgc
    34801 tgtacctaac ttgattgttc caaaatcaag atctggagct tggtcaatcg tcagtaatcc
    34861 ggatgttccc tgatttggtt cgtcaggtgt aaaaggagta tcaggatcat ttggatcaac
    34921 aggaggaact ggaacaacgt cttcaggatt tgcagcatct ggtttggtca cagaaatcgt
    34981 tccttctgta gtggctgagc tcttattctc agcaaaaaca ctttgtgtac ataataatag
    35041 acttccagct agaacggtac ttaatgcaat tgtttttttc atttcttttt ccttctttct
    35101 tttaaaataa ataatccagc aaataagcca ataagaatga ctccaacaag tatcagccat
    35161 cgccagtcaa atgtttctgg ctgatttttt aaatcaatat ccttttcatt caattggtcc
    35221 gctgcatcat cagttatttc aaaatcggta tggagattcc agcgtttttc tttccaagca
    35281 cccttcacaa ccgcttgata atgtcccgga gctaattttt ttccttctaa tgggacaagt
    35341 aaatcaaaat ttgtattggg tgctacatct attttttctt tttttattga aaggtaaggc
    35401 tcttttgttt cttttttgta gatttcaacc gatgtagtca agccgaacat ataagaagca
    35461 tttggatttt gaagattcgc cttaatcaca tttcttccat cgctttgatc cgcataagct
    35521 ttttttaatt tcaaatctgg ggaagactct ttttcgctat ttcaaatcaa gattgctttc
    35581 acttgagaaa actgattgac gacaccaact gtacttttgt tagaaacgtt ctcttttttt
    35641 tcttgaaacg tgatccctcc tgcgaccaca ccagccagct ctttttctgg cgctttcagt
    35701 gaaagtgtct cagtgaccgc actttgagga ggaacagtta tttctcccac tgtttttgct
    35761 aagtttttca tatcataact taatgtttca tcaaatttac tttttgattc tccatattcg
    35821 atcacaccgt ttgaattcgt cgtagcggag tggatcaatg tctcaactgt tactgattta
    35881 tctgttcggt taaagaccgt gtaggggatt tcctgtgttt tcccaggctc catccttaca
    35941 tttatatatg ttttgttctt atcaatctga ttagccggta attcaaaatc taccgcaaaa
    36001 ttgaattcgg aggcatggac aggtgtctgc caaaggatga atccaaggag aaggatcata
    36061 agtaaagagc agttgtttcg atgactttta ttccatattc gattcatcaa tcatcctcct
    36121 tgatctttat tctgtcggcg catctgctaa tgtccatgtt atctctgctg agtaattacc
    36181 agctatctga ttttctttgg ttaagacaag tttaaacgcc cgaccgttat ttaaatattc
    36241 tgaataatct gcttgcacat tcgttgaatt ttgtcctctg acaatttcca gttcttcttc
    36301 attcactaat tgatactcct ctcctttctt gaaatatagg acatcctgca tagaaaatcc
    36361 tgcatcattt gtgaaaggct gagataattt tatcgttaag atccacggat ttttcgtttc
    36421 tcgctgatca taaatccata atttttcctg tatatccatt tggccaaatg cttcttgtgt
    36481 caaaccagtt gctttgatct gaccaaaatc gagtttggaa ggggcaagaa atgagagacc
    36541 accttctact acgatcttcg tagcaggtgc aaaaggcggg gcatcatgat aaacaactgg
    36601 cgtatcactt ggattgtgat tcccaatttc atcgttggta cttggcttgt tctgcacacc
    36661 cttagcagta gcttctcctg cttggtcgtt taatacaatt tctaatacat ctcctgcaaa
    36721 aagttgattt tctgggatag aaagaacaaa ttcccctgta tcatcaatag ttgtttctga
    36781 caaaacagct tgattttttt tcaatgaaac caaggctcct gcttcacctg tacctgtgat
    36841 cgtgcgagaa gctgcattga tcaatgtggt acttaaatta gcaggttcag gaggaatcac
    36901 atcgactgtc gttttggcag agtccagttc tgtcacttct tggccacctc ttactttttt
    36961 agcagcaaca acttgcactt gatcctccgt ttttaatctc tcatcattgg gtacttgaac
    37021 ttcgaacaag ccgccttgtt cgccttctcc ccatgggtta tatcctgccc cttcattttc
    37081 atctgtttgt ccttgcgtga gagcagtaat ttctacagat gatttcgtcg tatctgcgta
    37141 agtaatacgt aaagtcactt ctacttcgcc tgtccaagca tctcttggct ttgcctcgac
    37201 accttcggga acaacgacat gcccatagat ttttttatct gcattcgttg gcactcttaa
    37261 ggattcaatc atcgggagct gattattggc actgattcga ttataatttt tcattccggc
    37321 acctgtctga gcaaattcat ttgttaaacc tgcactaggg cttgatacga aattgcctaa
    37381 gtcggtccca ttgatcgata agtctgtcat atcagaaaaa taatccgggt ctttttcaaa
    37441 gttatcgaat gttgcgcctg tttttcgcca taaagcgatt tctgaatggg cgatctttaa
    37501 agaagaatct ccatttcctt ggaataccca gccgcctatg ccattggtct cgcttccaat
    37561 ccgtttgttt tggaaatcaa aataggtagg agattctaag tccacattca acacatcccc
    37621 actgatgatg ccggtcttat cttcccctgt tttcccgacc atgactaaat aagttttttc
    37681 acctgccgta aaatttaaat caatatctcc agttgtatct acagttgctc cggaatctgc
    37741 gcgtatatcg atatttgatt tttctccagt gactgtaaaa tcagctgaac ctggactttc
    37801 tagaaaacca gaagcgtcat aaaattggat cgcttgattt ccttgattga gtctgttcac
    37861 gtaagatttg ccatcacctg tatttcttac cctcaatgag cctccattgg ttactgtcag
    37921 cttttgcgta ccattctcaa aacgaactgc gctggaacgc ccactctgct tatcgatgtc
    37981 gataactgat ccggctcctt ctacgatcaa agaaaggcct tttcctacaa aacgcatccc
    38041 attgttcccg ggttgatcgt catctccagc cattttgacc gagatttgtc caccacttga
    38101 tacatggatt tgactagaat ctccttgcaa gcgaaatccg cccgtagttg ctgtattgat
    38161 ggtgattttc ccaccattcg atacagtgac tttcgatcct gaacctctca tttggatgat
    38221 tccgcgattg ttgtcattgg taatattgat ctttgtattt accgaaaatt cgcttcccga
    38281 accactgact tcaaacatga cattaccagg tagcgtatcg actgcctctc cttcatcgct
    38341 aacgccatct gcccgatcat aatcaagaga gacctttgaa ccgcttaaga tatggattcc
    38401 tggcgctgtt tttccactac cagtctcatc tcctgaatct agaaaaccgt agaatttccc
    38461 tagatacggt ccataaagtt gactaccatt gatcacattc aattgatagc ttctacaaaa
    38521 ataggcttta cccggagcgg cattcagtcc ttccttcact ggttggacat ccaacacacc
    38581 ggaaacacca tctaatgtga cagttgcacg aggagcgtaa atcagtccta atttggaccc
    38641 atcatacaaa tcaagtgtcc cggtaaattt tatttctccc gtaaaatgat atctgcttcc
    38701 ccaaaaatcg gtcatataag caatgataat ggcatttcca tcgatatttg gtgtcaatcc
    38761 acctggatgt gcagctgttc ctttaaaatt ctggaactcg atcgactgag gtatctcatt
    38821 gatataaata tggtgtgtgc cgaaattgtg agcgaatcca ccaaaatcaa taacgatatc
    38881 tcgtttgatc tctaattccc tgtctaaagt caaaggctga atcagtcgga tcttagttac
    38941 tgattcgtct gcgagaagct gcgccagttc ttctgttgtg gcagcttcgc tgtacccatc
    39001 cgctgaaaca gctcttgcca gtgccgcttc actgctatca gacgtttgtt cagctacttc
    39061 tgaggtctca cttgtcttat tttcttcttg ccttttatta gtagaagcct cactttgaga
    39121 ttctgtagtt tcagaagtaa tcgattcttc tgaaaaagta gtcttgtcaa aactttgatt
    39181 ctctgattgc gttgattcgt ttgttttctc tacatcggca aaaacggatg tcaccatcaa
    39241 gagagggaag acaaacaaaa tcgacgcaat cagctttttt acacattgat tcattctcca
    39301 ccatcctctc tgacgttttg acaattttat tttatcgttt taatctattt ccaattgtac
    39361 tttcatattt aaaatatgta tttttatgga aaaatttttc tattaggtgt aaatttcttt
    39421 tttgtacaaa aaaaaggctg tgacaaaagt cttgtcacag tcccttatcc cgaacaagcg
    39481 gtgttctccc gccaactcct cggtcacaac ctccatttta acaataaacc tgtaaatgtg
    39541 agggcaatac tttgattgtc acaggtaatt tatccccttc atctccatca acattcgtcg
    39601 tcaattcttc ctcatcagct aatgacacaa ctaattgctc acaagtaagg taggctagat
    39661 ttttcgtaga ttcatctact ccttttaata ggtctggcaa tgttttaagc gtatcgatca
    39721 aagaagaatc ttttaaataa accaaatgga gcttaccatc atccactttt gcatttggaa
    39781 gtaatgtttc aaaaccgccg actgagttag ttagtcctat cagcaaggta ctgctctcaa
    39841 tttcttcctt ctttccatca acttctacgt gaaacgagta ggattgtgtg gaagctaact
    39901 gtttgattcc tgacatgaaa taagctagtt ttccaaattt cgtcttcttc tccgcatcta
    39961 catcattgat tgattcaggg atagagccaa ttgcgacgac attcataaaa tagtcgtcat
    40021 ttatttttcc aatatccaat ggcttgactg attcaatcga aaaatgattg atcgcttcct
    40081 caggttctag cggtatccct aatgctcgag ccaagtcatt caccgtaccc aatgggaaaa
    40141 atccgaaatt tggccgatgt tcttgttcag cgattccact gattccttcg ttgactgttc
    40201 catctccgcc catgacaaac acactatgat acccttccgt agccgcttcg cgagtaaagt
    40261 tttttgcatc ccctgctttt tttgtatgca agacgaccac ttcatcaaac accgattcca
    40321 attttgctat cgctaattgc tcaaattcct ttgcttgttc accgcctgaa ctggggttta
    40381 ccactaataa aacttttttc atcactttct ttcccttctg attgatttct ttaatataac
    40441 gatagcataa agaaaaaaat taggctgtga tgaagtactg ttcatgaaac attcatttat
    40501 tttttgacaa gataaaacat ccatactatg aaactgagga aaattaaaac aatgatatag
    40561 gaaataaaag aaaatgatag gcattgtata cctaataaag taaaaatctt aggtggcaga
    40621 taaaaacaca aaaatagcaa gagaatgtaa tagcgtttct tccacttttt gttatcagac
    40681 cttatagcga tcagaggaat accaaatgca taactgaaaa actcttgatg tactgttaat
    40741 atgtcacggc cttgaataat aaatgcgaca atccccgcta taattagtgt gcctaacacc
    40801 aaaacggaaa attgatgaat atattttata tacaaatgat ccattgtact tcctgccttt
    40861 ttatttgtga gaaaaacaaa tccgtatacg tgaataactt ctagaaataa aagaaatttg
    40921 caaaaccact ccatttatct acttctacgt gcattttttg atattgtttt ctttatttaa
    40981 gattatacag aaaaatatct agggtcgctg gattctaagt aaaaaaaatt tttaaaataa
    41041 aaagaactaa atcaagtagt aattaaacta ctgttgattt agttctaata aaaagattta
    41101 gatgaacagt tttattgatt taaatagttc atatttttta tttataccgc ttatttacta
    41161 cttgctgtac tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg
    41221 atttttacat tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca
    41281 cttaattttg tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg
    41341 cctttgtttt ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca
    41401 ttggtagaag tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg
    41461 acttctgccg gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt
    41521 gtcgtagtat cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca
    41581 gctttcttca catcttttgc atcatcttca cttgctactt ggataatttg agcttccact
    41641 tctggatgga aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc
    41701 tttagacctt cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg
    41761 taacctgcag atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca
    41821 tattgagaat cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga
    41881 taaacgatca actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt
    41941 acttcttgtt cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc
    42001 gtacagcctg acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc
    42061 atttatcaat tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga
    42121 tgaaaatcat caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc
    42181 tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat
    42241 atactacttc ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact
    42301 aattcttaga acaaatgtta tcgattttgt ttttcttctt ccgctgctgg gatattcaaa
    42361 tcagacactt ttatgttcga ttttccagac atttttttgt tgtttttatt atgatatatt
    42421 tttatacaac taaaaatgaa tatattaaaa ctaccaaaaa taacttttaa cgctgtttca
    42481 ttatatgttt tttctatttt atataaactt ttggatactt aataccaacc ggttggattg
    42541 taccaacatg agaatgtgtc agttctattt cctgtcgcac ttgcataggc ccatactcct
    42601 tttgttgcat ttgctgatcg aatagagttt ccaactttcg cttttgcacc gtgacgatta
    42661 tagacacgac tatagtggtt agaatgacct gtttttctcg tacaagttgt atcactataa
    42721 taaacccatc ttccagagct atgattaatt gctgttctag aaatttcccg gtctactggt
    42781 aaatttgact taataatatt acctttagca tcaaaatgaa tctctcctgg ctttaaagat
    42841 ttgtaataga tttcaccgtg aactgaattt gcttccactt cagatgacat agcgtttcct
    42901 ccaacaaaac ctatccctag tgcacaaata ataactgctt ttttcataaa acgacatcct
    42961 ttcctttttg atttttaaag catgatcttc ttgtttccta ttttggtcag atatggatca
    43021 tgcgtgacta cgataactaa cgtaccttgg gaagcttgtt gcgataagat ttcaactact
    43081 ttatccttat ttcttttatc caatgaccca gtcggttcat cggctaaaat gattttgcta
    43141 ttcttcaata agactctcgc acaggcgatt ctttgcttct caccaccaga taatgtgtag
    43201 ataggcgtac ttagcttctt gtttatattt acttttttca atgcctcata ctttgctgtt
    43261 cgcttatctg aaacatttaa attcgaaaac ttcaatccaa tatctaaatt aaaatcgatt
    43321 gtttcatatt caatcaaccc aaaatcttga aaaagaaaag atatggtatt tctttttatc
    43381 aagcgcttct ctctcccagt taattgtttc gtctcttgat cgaacaaata gaattgtcct
    43441 gaactgaact gatcattcaa tcctaatata ttcaataacg tacttttacc tgctccactt
    43501 tctccaacaa cagtcacaag atccccagca tccgcagtga aacgatccaa atgaagaatc
    43561 gttttgtttc gaatgatttt tttcgcacct tttatcacta tcaacggctc caattgttaa
    43621 cctccattca tcagcaataa ctgacttttc ttttctgtag acaaaataaa aacaactgaa
    43681 agaataaaat caatgaagct aaagaaaaaa atattaaaaa gatatttagg ctctctttct
    43741 ataagaaaaa ggaagattac acttgtcgtt ccccatttga tgaggttcat catgaagaaa
    43801 tctttatacg tttcaaagag cgaataacca cttgaacgaa agatagcgat tttccgacaa
    43861 ttgtagacaa aatatatgca taacatgaca tagctcaaca agaagaaaga aaagaaagtg
    43921 agtaaaaata tctgtatcaa gtaatgaaat gatctgctta acactttgac agctatctct
    43981 tcagtctgag tatacggaaa tgataactgc aagttatcac gtaactgact ttcttcaatc
    44041 agctcaaata acctttcttt cgacaaagag cctatcttga tctttaacgg tggatagatt
    44101 tctccgctga gaatatttct ttccaaatac atactgtttt tcaatgtcag tatttcaaca
    44161 accggataat gttcgatcca ggggatagaa ggaataaatg tataaatggg ttgattgttt
    44221 tttataaata gaaatttcgg ttctttttca ctgatttctt gataatattc tgttatttgc
    44281 gccaggtcat tttttaaatc tgtatgtcta tatcgttcag ggatcaatat aacagggtgc
    44341 gtttcttttt cggttatttg gatcaacctt ccagagacat caatcaaagg aaatttctta
    44401 agataattca gatttatttt tatccctctg ccaaactgtt cattttcttc attcaagtaa
    44461 tctgatacat tgactaatat acttccgttt ttattcaaag tttcatataa ttcctgatct
    44521 gctgctgatg caaaacgatt ggaataaata aaatccacgt ggtttttccc gatggttact
    44581 ggataaaata catgatagtc ctcaccagcg atttcttttg gtggttcatg ggaaacagct
    44641 aactgtgaag aagaaaaaac gatagttgct aattcgacat tggcaacggt caaaagaaga
    44701 gaaaacaaca atccaaatgt aaaaagaatg tattggatat agttgaacgc ttctcttaac
    44761 gaagcgattt tttcaactaa taaaagagaa atcaaagaga taccatatat tccgctgaac
    44821 aagaaaacgt acatctggaa caaataaaga aattgctcct tataatacat aaaaataaaa
    44881 ccaccaatga ttcctgcaat tatgaatgta ataatgattt tctttttccc caataaacac
    44941 cacaatactt gatgtaatga aaagtcatta ttcaacaata aactgatttc tttattcatc
    45001 aataagaaaa aggatgataa caaaaggaat aaaagaatca atccaatagt taaatacact
    45061 cgataattat atacgtctgt atttgaaata accgaatacg aatagctatt ttcaaagtct
    45121 tcaaaaatat tagtggcata atcagtgtga aatgcttgat tgaatcgatc agtgaatagt
    45181 gaaatgaacc gttcataggc ttctttttct ctcgtcatca agaaaaattc accttcaaaa
    45241 tcttttattt tgatcgattc ttcaatggac gttacgcgaa aggaggtatt agaatagggt
    45301 accttctcgt gagaagattt gacatcccct tgataataca atgtcatttc aagctttggc
    45361 tgtatcttga agttccaaga tgtatctact tcattggatt catttactgt ttttttcata
    45421 taagaaagct cagtttctga tgagacattt tcaaatacca tgattagctg ctcatattct
    45481 tcttgcccaa taaaatgttg tggcaatcga atcggagtat aagtatctgt ttgatggaaa
    45541 aattcatttg tccgatagag tacctgttta tttatactaa ccaaggcaaa accactcatg
    45601 ataccggtca ctatgattgt gagtaaaact gtgaactttt ttttgattat caccactctc
    45661 ccttacttaa atgattcttt gataaaatca taccgttttt catcaaaaaa acgtatctat
    45721 caagtgtata ggtcatgtag aataaatcta tatactgtag aatcgaaagg agaagaagat
    45781 gaacaccgga gaaaaaatac gaacttaccg cagaaattgt aagcttacgc aaaaagaatt
    45841 aggcgaaaaa attggtgttt ccgataaaac gatttctagt tgggaaaatt ctcgtacaat
    45901 gccagacctt gaaatgctgt cacttttaca tcatacttta ggtctgccga ttgattttcc
    45961 ttctattgaa tcagctaccg aatcaataga accaacattg ccagatgaac ctcttgttaa
    46021 caagaaatct tattcatcaa aacaaccctt ctcactcaaa aagacactat ctttctttct
    46081 gctcgtcttt tgtttggtct atctttttct tatcaattat caatacaaaa atgaaacgat
    46141 cgatcgattc aatccttttt tgaaaccaca aatcggctat accacactcc ctgtaggata
    46201 cgtggacaat tccatcgaac gttgggtatg taatgatatc tttggccatg gtacatggct
    46261 gtcttttcac gctggagagg tagaaaaaga gaaaaaccat gctgtaatca aacacaaagg
    46321 aaaacacgtc atgaacgttc gcctgctcac ttgggaagcc attccctcag attaccaaga
    46381 aatcctcagc caagactatg acgacgcctt aaaaagaagc cctcaggagc cagataggca
    46441 ttaagtagga gatgaacgta gagatagaaa aaatcaatgc acatgatctt atccaaaaaa
    46501 caggggtaca aattaattga aaaagccttg agatgaaagt tcctaaataa aaaagttcag
    46561 aatgataggt atttctggct tatagtataa tatttacgcc acagcctccc aatgattctt
    46621 tttggaatca taatcaagga tggacattca caaaaatatt ttgctcattc gatccgaata
    46681 gattaacgac caatctagta agtaatatag attggtacgt agtcaagctg cacataattt
    46741 agatcaattt tagttctttt ttatatttct atgatctgct catttacttt tatatgatgg
    46801 cttacagtaa cataaataaa tatgataatc gctggtagtt tatccaagag tactcaataa
    46861 aatacaaaat ggattgttca agcgcctatc ataatgataa aatgaaacaa ataaacgata
    46921 caaggaggaa gagactttga gtaatggcag aacaattttt tcattgtaaa aattgttcag
    46981 ttgttaatct atgggatttc ggtagcatgg gcattttttt atagaggaat cacaccaacc
    47041 tggccgtttt atctcttgtt agtgggaata ttagtaggaa attttgcccc tcttcactat
    47101 cgtacgaact tttttccaac tgattctggc ctttttctta aaacaaagac gcgaaaatta
    47161 gaaaacatta ttgaaatgac aattacctgt ataatcattt tcttagttat attcctaaac
    47221 gtcttctaat aagaatctct taaaattagc ttgttacagt tagttaccta gtttatataa
    47281 cacagttatc ttatcttcct tatcaacatc ttttgccaac tctttgtctt aaattcgaaa
    47341 catataagca gagctacgaa caccaagact gtttcggctc tgttttttgc atcaaattca
    47401 ctcttatata gctgataatc tcggctcttt aaaagaaatt attttcgctt cattttatta
    47461 taaaaaggct aggaaccttg atgttatcag gttcctagcc tttatttaag tattacccca
    47521 cagacccttc catatagatt tttaaattac acactagttt aaactaaaac aaacaaggaa
    47581 aaacttacca tttaatcaaa aaattagctc atatgaatat attagaatgt aaattagact
    47641 ggtcaaaatc tggtcaaaaa agaggacgaa tacaggtagt cctctttttt gttattttga
    47701 tattctaaat cattttttat tggattattc ccttgttcga tatagaccca aattgatcta
    47761 tcaataatta tcaaactgag tcatatagag acaaaaattt caaaaatgac ctgttaaatg
    47821 gtcatcgaat atcatttaaa atcaatacga cggagtcaga cacggagtca aaacaagccc
    47881 gctgagagta aaaaatatac agtcccatag gtaaagtgcc acaggactgt atattttttt
    47941 atttgagatt acctgaaatg attccattta atttttatct cattttttcc tactagttag
    48001 tatcaaatac tctcctattt tctcaataaa ctattttggg ttacatggta tctgaaacag
    48061 ctaaaacctt gtcattatag caaaaaatca ctttatctcc gtttcatttc agatacattt
    48121 cacttaagcg gattccaaaa aaacaaaaga aaaaggaacc cctagttagc ctatgctttt
    48181 tattggttcc tctcaaaaca cggtcatata acaacggggt ggggtaaatg aatgaaaaag
    48241 gtggaggaaa cattattcaa tagctgtctt aaatacttcg ttataaaatg tgaacaaata
    48301 aaagacatct atccctattt tctacacttc cacttgacta ccatctttga atcgtacaat
    48361 tttcttaact cttctcgttt acctcaattc attctcaagt ggaatcaact catcatctaa
    48421 cattttacgc catgctcgct tcatttcttg tgggtattta aatggcttcg cttctggatg
    48481 tttggcttta tatccttcgt aatccttact gttgataatt gctttcatat taggaattgg
    48541 atcttcacct ggtagatatt gcagattaga agcatgtaat tctttctcag aaacaaacca
    48601 ctcattggaa aagacatgaa tcaaatcatc actcgcttgt gtaaaatagc gacgtttaat
    48661 gacaaagata tcttcattac tatctaaagt ccccacatca aatgcattta aaatttgtcg
    48721 gtaaacttct ttgactcctt ctgttttatt caatttagcc aaagcttttt caatttcttc
    48781 tcgtgcatct gaactattcg cagcataagt tcctagtaat tgatcaatat aagttgaatc
    48841 aatatcatac aacttagtgg tattttcact ataaaattcg atcgttgaaa aatcttctcc
    48901 ttctttttct ggttcttcct ctattaatga acccttcacg gtctcgtaca cgccaatttg
    48961 ttcttccaaa agttgaattt gattgttcaa agttggagat ttcaccatgt catcattgta
    49021 atcgtcataa gtcactaacg attcataagc acgattcaat tcttgaaatg ctttgacgta
    49081 ctcgatacgg atttcaagtg gcgtatgttc atcaatggat tccatggatg aaacctcttt
    49141 taacttacca tatgcttgtt tgaatctttt tttggattcc tgataacttg gataaatcaa
    49201 acctgattct tcttttgctt ttgaatacag ttttgtcgca tcctcgacat tcttctccat
    49261 tgtcgctggc ttacgaaacg tcacaatcaa tccttttgtc ttatcaggat acgtacgatt
    49321 cgtacgtgaa aaagcttgga tcaatcctgc atactctaga ttacgatcca caaatagggt
    49381 ttgaatcgta ggggcgtcaa aacctgttaa tagacgatcc actacaatcg ctaaatcgat
    49441 ttgtttccca aattgtttga attccgctct ttttcgtgcc aaacgattat tgatgtctcc
    49501 gttataacgc tcaatgtctg ctaatgacca ggaagtatca taatactgat tatattcctc
    49561 aataattttt tgcatttctt cttgttgggc acttgaatct cggctatttt cttctaatga
    49621 ataggtaata gcgattcgtg gaaaatcagg atcctccatc gttcgtcctt cacgaatgag
    49681 ctgattggga aactcttttg ttaaccaatc aggctctttc gtcatctctt tgatggcccg
    49741 ataatacttt ttggccatgt caatggaact cgtggttaag attgcagatt tcgttggcct
    49801 gccatttcga aaatcaaatt ttgtataggc attatctgga cggaacattt tatgaatgac
    49861 tttttggata tgttcatctg cttcaaaaac agctgggtcc aaataacttt cttttttgac
    49921 aggttccatt tgatcaatca tacgattgat ctgttctgat gaatactctg catatgtttc
    49981 tacttctttt aatttccgat agattttatt ctctagtgaa gtcggctcca cggtgttttc
    50041 atgctctact tgaaatccta agacagagcc atcttctagc gcattcttaa tggtataggt
    50101 atgcaagacc tctccatact gatcatgggt tgtacgtgcc aattgccctt tggcttgttt
    50161 ccgattctca ggaaaaatcg gtgtacctgt aaagccaaac caagtggatt tcggaaacat
    50221 ttttttgatt tcttccatat tttcagcact taacgctcga tgacactcat ctactacaaa
    50281 cacgatatgt tggcccatta atttttggaa gcgattggtt ccttttttct cttcttgttt
    50341 ctttgcatat ttcaacgccg catctagttt ttgacgggtt gtaatgatta caacatttgc
    50401 attggcatct gccaacaggg tttcacttaa ttctttggca cttcccgttc caacgatcaa
    50461 gctattcgct ttggcgttcc cggaagaaat cccagtatta aattccgaag caaattttgt
    50521 aaactctgtg gtggtttgat tatctaaatc tttgcggtcc actaacatga tcgttcgatc
    50581 gatccccgat tttcgtgcta aaagcttagt agatacaaaa ctagtcaacg tctttcctga
    50641 accagtcgca tgccacacat accctgattg atgtttattt gcggcaataa acaaggcttg
    50701 aatcgcatgt acttgataag gatgtaacac catgagggtt ttattgtctt gatcttcact
    50761 gacgattgta taatccgcaa tcaaacgatg agcatccggg atatttaaca cttgtttaca
    50821 aaactcatat aagttttcta ctttttcatt gtctttcgtt cgccaactaa ataaaaactt
    50881 agggtgcaag tctttcggta aggcatttgc aaaataccga gtggtttgtt cattggaaac
    50941 cacaaacaac tgtaaggtcg aaaagatatt gttccgaaac ataccttctt ctgcatattt
    51001 tttgatttgg ttaaatgcct gataaacacc atcttttgca gttacttgtt tcagctcgat
    51061 ttggacaatc ggtaatccat tgattaataa ggtgacgtca aaacgtcgat cccgtgcttc
    51121 tatgttactt ccacgttttg caatttgatg gaccacttca taagtagaga ttcctccacc
    51181 aatgtcttga ttggaataaa ggataagtga gacagaaccc aattgcgaat cttcccgttc
    51241 aatcgtgatc cgagccatcc cattttcacc ttttaaccat ttcgcagcat caaatggggt
    51301 tttcgttcgc agcagtaatt ccgttttgat cgtttcaaat tctttatcgg tcaaaggaaa
    51361 atcattcagt tctgcttgat tatttgagat aattttttgg cgtaaattga cccaaaggtc
    51421 ttcttctgat tttagatccg gacgatacgt ccactgatta tgcccttctc caagtacacg
    51481 aatcaactgt tcttcgactt ctgcttcctc acggtgacta attttcattc ttcttccctc
    51541 ctagacaaac atcttttgta agaagctttt tttcgtttct tttagtaaat ctaacttacg
    51601 ttgatgaaga gcgatcgtat catctagctg tttgaagaaa gtaccgattt tttgttgttc
    51661 atcctttaca ggaaccttaa cactgatatt ctcaatatca gaactgttat aagaaggcaa
    51721 tgcccctact tgggagtatc ttggcaaatt tatagtttca aacaatattt taccaaaata
    51781 gatatcccaa gtattattaa ataaataagc catggtattg ttatctatta agaaagggct
    51841 tgtgaccaaa cgttttctat taagcattat tgctgcgcca actttagcaa atataatcgc
    51901 tggcacatct tttattggct tccatttttt acgctcaatt tgctcattag atacataatg
    51961 atttgcattt ctcatttcat actcattacc aatgttattc atgtcggaaa ctttaaaaaa
    52021 tggagtacct tcagaaccac cttgctcggc atcaggaaat ccaattcccg attgagtttt
    52081 acctaattct cccaacttac gctcttccca atcttccgta aaaccaggaa accggatttc
    52141 tggcactttt gctccgtttt cagggaacat tttttgtaag aatccttttt tcgtttcctt
    52201 cagcaaatct aacttacgct gatgaagagc gatcgtgtcg tcaagttgtt tgaagaaggt
    52261 gccgattttt tgttgttctt cctcactagg aaacattgct actatatttt taaacttttt
    52321 tagagacaga acatgttgac ctgttccctc tgcttgaata gataattgtc gttggattcc
    52381 attgttaata cgtcttaaga agaagtcgga atcagcagat tttaaagtaa atacaggata
    52441 aaaatagctt ataatccctc tatcaataat gtcattacgg ttaaacacaa acacatcatt
    52501 atcacttcga ctacggaacg tgaaatatcc tctaggtagt acaaagtaac caatattatt
    52561 ttctgtagtt acttgccgat ttgcaaaata gtcttcttgc aaaactatcc ctttttgttg
    52621 agatgaagtt agcataggaa attgattttg tacacttgtc ttttcaacat aatctacaac
    52681 gaagtccccc aacttcctct gttcccaatc atctgtaaat cctgggaaac ggatttctgg
    52741 ttgtgtatca ttgctcatgt ttgaacacct ccagcgcacc ttgtaaccac gcttcatttt
    52801 caggtgttgt ttgcagggaa gagatagctt ctaaaagttt tttttctagc acttgctgtt
    52861 cttgtcgtat cttcttgatg tcatttccga catgaaccat atcaattggt tcttcttctt
    52921 cgaaagtatc tacatagcgt gggatattta agttatagtc attttctttg atttcatcaa
    52981 aggttgccaa atgagcgtat ttctcgacat cttttctttc agcgtaggtt tcgaggattt
    53041 tttgaatgtt ttcttccgat agtttatttt gatttttccc tttaacaaat tcacgactag
    53101 catcaatgaa taatacatct cgagtttgac gattcttctt caacacaatc accgtcgttg
    53161 gaatcgatgt cccaaagaac aagttcgcag gcataccgat cacagcataa atactgccat
    53221 cttctagtaa tttttgacgg atgacccctt ctgcagcgcc acggaataaa actccgtgtg
    53281 gtaagacgat ggccatggta cctgtttcct tcaaatgata gaacccatga agaagaaacg
    53341 caaaatctgc tttggatttt ggcgctaatt tcccataacg attaaaccga gaatcatcta
    53401 agaaagtcgt atctgctgac caattagccg aataaggtgg gttcattact actgcatcaa
    53461 atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt atccccattg cggagattca
    53521 tttcttcggc gtcgacacca tgtaaaatca agttcatttt tgctagattg tatgtcgttg
    53581 tatttagttc ttgcccatga tatttaacgt tatctgggtg tgttaagtag ttacgtacgt
    53641 ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac actaaagaat cgtcgttcct
    53701 tttgatcgag cgttacgatt tgcgccatca tatcagaaac catatgaggt gtgtaaaatt
    53761 ctccggcttt cttccctgct tctgaagcaa attgactgat cagaaactca tacgcatccc
    53821 cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt caatttcttg atgacttcgg
    53881 taatcgtcac atttcgttgt tgctcatctg ttcctagttt ttttgattgg agatccacat
    53941 catcaaataa cccattaaat tgattgtagg tagatgccaa ttgcacaaag gctttgttta
    54001 gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc taagacgttg aaaagatact
    54061 ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc aatcagatct tcttttgatt
    54121 cttcatctga aagtaattca cgatacaaca ttgtttgttt gcttactgtg tcatattc
//
LOCUS       DAJSBC010000014        48459 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSBC010000014 DAJSBC010000000
VERSION     DAJSBC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.014"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..893
                     /locus_tag="ONT65_000810"
     CDS             <1..893
                     /locus_tag="ONT65_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2328460.1"
                     /translation="PLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEII
                     LSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASI
                     GLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKY
                     GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKY
                     CANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVC
                     QTRRLNSRIDKVRYMFNKIT"
     gene            991..1179
                     /locus_tag="ONT65_000811"
     CDS             991..1179
                     /locus_tag="ONT65_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2328461.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1237..1590)
                     /locus_tag="ONT65_000812"
     CDS             complement(1237..1590)
                     /locus_tag="ONT65_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328462.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2121..2543
                     /locus_tag="ONT65_000813"
     CDS             2121..2543
                     /locus_tag="ONT65_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2328463.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2540..2770
                     /locus_tag="ONT65_000814"
     CDS             2540..2770
                     /locus_tag="ONT65_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328464.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3268..3468
                     /locus_tag="ONT65_000815"
     CDS             3268..3468
                     /locus_tag="ONT65_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2328465.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3495..4688
                     /locus_tag="ONT65_000816"
     CDS             3495..4688
                     /locus_tag="ONT65_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2328466.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4756..6321)
                     /gene="guaA"
                     /locus_tag="ONT65_000817"
     CDS             complement(4756..6321)
                     /gene="guaA"
                     /locus_tag="ONT65_000817"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2328467.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6380..7303)
                     /locus_tag="ONT65_000818"
     CDS             complement(6380..7303)
                     /locus_tag="ONT65_000818"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR2328468.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7499..8104
                     /locus_tag="ONT65_000819"
     CDS             7499..8104
                     /locus_tag="ONT65_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2328469.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8412..9428
                     /locus_tag="ONT65_000820"
     CDS             8412..9428
                     /locus_tag="ONT65_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2328470.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9569..10870
                     /locus_tag="ONT65_000821"
     CDS             9569..10870
                     /locus_tag="ONT65_000821"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR2328471.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10916..11578
                     /gene="deoC"
                     /locus_tag="ONT65_000822"
     CDS             10916..11578
                     /gene="deoC"
                     /locus_tag="ONT65_000822"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR2328472.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11599..11997
                     /locus_tag="ONT65_000823"
     CDS             11599..11997
                     /locus_tag="ONT65_000823"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR2328473.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12063..13145
                     /locus_tag="ONT65_000824"
     CDS             12063..13145
                     /locus_tag="ONT65_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2328474.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13357..14919
                     /locus_tag="ONT65_000825"
     CDS             13357..14919
                     /locus_tag="ONT65_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328475.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14912..16045
                     /locus_tag="ONT65_000826"
     CDS             14912..16045
                     /locus_tag="ONT65_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328476.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16045..17001
                     /locus_tag="ONT65_000827"
     CDS             16045..17001
                     /locus_tag="ONT65_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328477.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17100..18266
                     /gene="deoB"
                     /locus_tag="ONT65_000828"
     CDS             17100..18266
                     /gene="deoB"
                     /locus_tag="ONT65_000828"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR2328478.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18290..19108
                     /locus_tag="ONT65_000829"
     CDS             18290..19108
                     /locus_tag="ONT65_000829"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2328479.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19125..19832
                     /gene="deoD"
                     /locus_tag="ONT65_000830"
     CDS             19125..19832
                     /gene="deoD"
                     /locus_tag="ONT65_000830"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2328480.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20130..20816)
                     /gene="gpmA"
                     /locus_tag="ONT65_000831"
     CDS             complement(20130..20816)
                     /gene="gpmA"
                     /locus_tag="ONT65_000831"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2328481.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20988..21569)
                     /locus_tag="ONT65_000832"
     CDS             complement(20988..21569)
                     /locus_tag="ONT65_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2328482.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21702..22385)
                     /gene="rpiA"
                     /locus_tag="ONT65_000833"
     CDS             complement(21702..22385)
                     /gene="rpiA"
                     /locus_tag="ONT65_000833"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR2328483.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22704..23117
                     /gene="rpsL"
                     /locus_tag="ONT65_000834"
     CDS             22704..23117
                     /gene="rpsL"
                     /locus_tag="ONT65_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR2328484.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23201..23671
                     /gene="rpsG"
                     /locus_tag="ONT65_000835"
     CDS             23201..23671
                     /gene="rpsG"
                     /locus_tag="ONT65_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR2328485.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23765..25852
                     /gene="fusA"
                     /locus_tag="ONT65_000836"
     CDS             23765..25852
                     /gene="fusA"
                     /locus_tag="ONT65_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2328486.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26024..27211
                     /gene="tuf"
                     /locus_tag="ONT65_000837"
     CDS             26024..27211
                     /gene="tuf"
                     /locus_tag="ONT65_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2328487.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27618..27926
                     /gene="rpsJ"
                     /locus_tag="ONT65_000838"
     CDS             27618..27926
                     /gene="rpsJ"
                     /locus_tag="ONT65_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR2328488.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27957..28586
                     /gene="rplC"
                     /locus_tag="ONT65_000839"
     CDS             27957..28586
                     /gene="rplC"
                     /locus_tag="ONT65_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR2328489.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28614..29237
                     /gene="rplD"
                     /locus_tag="ONT65_000840"
     CDS             28614..29237
                     /gene="rplD"
                     /locus_tag="ONT65_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR2328490.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29237..29527
                     /gene="rplW"
                     /locus_tag="ONT65_000841"
     CDS             29237..29527
                     /gene="rplW"
                     /locus_tag="ONT65_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR2328491.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29566..30399
                     /gene="rplB"
                     /locus_tag="ONT65_000842"
     CDS             29566..30399
                     /gene="rplB"
                     /locus_tag="ONT65_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR2328492.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30442..30720
                     /gene="rpsS"
                     /locus_tag="ONT65_000843"
     CDS             30442..30720
                     /gene="rpsS"
                     /locus_tag="ONT65_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR2328493.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30743..31090
                     /gene="rplV"
                     /locus_tag="ONT65_000844"
     CDS             30743..31090
                     /gene="rplV"
                     /locus_tag="ONT65_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR2328494.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31104..31760
                     /gene="rpsC"
                     /locus_tag="ONT65_000845"
     CDS             31104..31760
                     /gene="rpsC"
                     /locus_tag="ONT65_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR2328495.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31763..32197
                     /gene="rplP"
                     /locus_tag="ONT65_000846"
     CDS             31763..32197
                     /gene="rplP"
                     /locus_tag="ONT65_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR2328496.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32187..32375
                     /gene="rpmC"
                     /locus_tag="ONT65_000847"
     CDS             32187..32375
                     /gene="rpmC"
                     /locus_tag="ONT65_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR2328497.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32400..32666
                     /gene="rpsQ"
                     /locus_tag="ONT65_000848"
     CDS             32400..32666
                     /gene="rpsQ"
                     /locus_tag="ONT65_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR2328498.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32724..33092
                     /gene="rplN"
                     /locus_tag="ONT65_000849"
     CDS             32724..33092
                     /gene="rplN"
                     /locus_tag="ONT65_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR2328499.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33129..33437
                     /gene="rplX"
                     /locus_tag="ONT65_000850"
     CDS             33129..33437
                     /gene="rplX"
                     /locus_tag="ONT65_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR2328500.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33465..34004
                     /gene="rplE"
                     /locus_tag="ONT65_000851"
     CDS             33465..34004
                     /gene="rplE"
                     /locus_tag="ONT65_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR2328501.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34023..34208
                     /locus_tag="ONT65_000852"
     CDS             34023..34208
                     /locus_tag="ONT65_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR2328502.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34244..34642
                     /gene="rpsH"
                     /locus_tag="ONT65_000853"
     CDS             34244..34642
                     /gene="rpsH"
                     /locus_tag="ONT65_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR2328503.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34674..35210
                     /gene="rplF"
                     /locus_tag="ONT65_000854"
     CDS             34674..35210
                     /gene="rplF"
                     /locus_tag="ONT65_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR2328504.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35371..35727
                     /gene="rplR"
                     /locus_tag="ONT65_000855"
     CDS             35371..35727
                     /gene="rplR"
                     /locus_tag="ONT65_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR2328505.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35748..36248
                     /gene="rpsE"
                     /locus_tag="ONT65_000856"
     CDS             35748..36248
                     /gene="rpsE"
                     /locus_tag="ONT65_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR2328506.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36263..36442
                     /gene="rpmD"
                     /locus_tag="ONT65_000857"
     CDS             36263..36442
                     /gene="rpmD"
                     /locus_tag="ONT65_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR2328507.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36486..36926
                     /gene="rplO"
                     /locus_tag="ONT65_000858"
     CDS             36486..36926
                     /gene="rplO"
                     /locus_tag="ONT65_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR2328508.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36926..38221
                     /gene="secY"
                     /locus_tag="ONT65_000859"
     CDS             36926..38221
                     /gene="secY"
                     /locus_tag="ONT65_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR2328509.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38283..38930
                     /locus_tag="ONT65_000860"
     CDS             38283..38930
                     /locus_tag="ONT65_000860"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR2328510.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39121..39339
                     /gene="infA"
                     /locus_tag="ONT65_000861"
     CDS             39121..39339
                     /gene="infA"
                     /locus_tag="ONT65_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR2328511.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39372..39488
                     /gene="rpmJ"
                     /locus_tag="ONT65_000862"
     CDS             39372..39488
                     /gene="rpmJ"
                     /locus_tag="ONT65_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR2328512.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39506..39871
                     /gene="rpsM"
                     /locus_tag="ONT65_000863"
     CDS             39506..39871
                     /gene="rpsM"
                     /locus_tag="ONT65_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR2328513.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39899..40288
                     /gene="rpsK"
                     /locus_tag="ONT65_000864"
     CDS             39899..40288
                     /gene="rpsK"
                     /locus_tag="ONT65_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR2328514.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40368..41306
                     /locus_tag="ONT65_000865"
     CDS             40368..41306
                     /locus_tag="ONT65_000865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR2328515.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41334..41714
                     /gene="rplQ"
                     /locus_tag="ONT65_000866"
     CDS             41334..41714
                     /gene="rplQ"
                     /locus_tag="ONT65_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR2328516.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41986..42825
                     /locus_tag="ONT65_000867"
     CDS             41986..42825
                     /locus_tag="ONT65_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2328517.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42801..43670
                     /locus_tag="ONT65_000868"
     CDS             42801..43670
                     /locus_tag="ONT65_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2328518.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43660..44457
                     /locus_tag="ONT65_000869"
     CDS             43660..44457
                     /locus_tag="ONT65_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2328519.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44660..45406
                     /gene="truA"
                     /locus_tag="ONT65_000870"
     CDS             44660..45406
                     /gene="truA"
                     /locus_tag="ONT65_000870"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2328520.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45739..48408)
                     /locus_tag="ONT65_000871"
     CDS             complement(45739..48408)
                     /locus_tag="ONT65_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR2328521.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15622 a         8899 c        10353 g        13585 t
ORIGIN      
        1 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
       61 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
      121 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
      181 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
      241 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
      301 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
      361 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
      421 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
      481 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
      541 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
      601 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
      661 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
      721 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
      781 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
      841 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
      901 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat
      961 ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct
     1021 atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct
     1081 ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata
     1141 acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat
     1201 cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc
     1261 attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat
     1321 gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa
     1381 ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg
     1441 ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca ttgttcccac
     1501 ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac caacggctct
     1561 aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat tttactgttc
     1621 ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt tcctatagaa
     1681 cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg ctgattttat
     1741 ttacctatac aactttataa aatttagtca tactcctaga aagtagataa aattggcgac
     1801 ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct cttaattaag
     1861 ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa tgtggttttg
     1921 cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct gtttatgtgc
     1981 ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca acaaatcccc
     2041 cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact ccctttcacg
     2101 acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata gaaaatcagt
     2161 ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc aaacatctag
     2221 acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt gtcaactctt
     2281 tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt ggattaagaa
     2341 tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag aaaaaacgga
     2401 acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg gagttactaa
     2461 atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg attaaagaat
     2521 tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc ccctcattgt
     2581 aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt atcgagggta
     2641 tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc atatggttat
     2701 tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt tatcgttcga
     2761 aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc ttcccgattt
     2821 gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga taggctaaca
     2881 agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt aagaaaagag
     2941 ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt agtaattcga
     3001 aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga tactcccgta
     3061 tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca tgagaaccat
     3121 gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga tattcttctc
     3181 ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt ggagttttcc
     3241 tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga tttagttgag
     3301 ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga attgatgatt
     3361 acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg tgaagctgtt
     3421 gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt tttgcacagt
     3481 aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga acctattatt
     3541 ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat cgtagtggat
     3601 tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg aaaccagaag
     3661 aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa attttccttc
     3721 catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca actattaaga
     3781 agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt gatgtgcaaa
     3841 attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct gttcaaggac
     3901 tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca aatccatcta
     3961 agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca aaagaagaat
     4021 ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt ttatttatct
     4081 ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc attcaatggg
     4141 aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat tacaagaatc
     4201 aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat attgtattag
     4261 ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat ctaattcaga
     4321 cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta tcatatgcta
     4381 ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg ttacggcatt
     4441 ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa gatcgtctag
     4501 gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct aacagtaatt
     4561 tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa caaaattatg
     4621 atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa atgaaaaaag
     4681 tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat aagaggattt
     4741 ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag tgatgtcata
     4801 cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt tttgcaagac
     4861 ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta cagcacggat
     4921 accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc ggatccctgg
     4981 aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga tttcttcacg
     5041 caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt cgccaagaac
     5101 acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat caggcatgcc
     5161 taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa
     5221 ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga tcgtctgtgc
     5281 tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta ggaaagagac
     5341 gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga attcattccc
     5401 aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta agaagcgatc
     5461 tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt ccattacttg
     5521 ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca attggtcacc
     5581 gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg aaagaccaag
     5641 caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat cgataaagtt
     5701 atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc gcagcaattc
     5761 gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa aattacgtgt
     5821 tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt caaagccttc
     5881 aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt ttggtgtttc
     5941 tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat attcgcggtt
     6001 tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac catagcaaat
     6061 ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg cattctcatc
     6121 atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg cttttacttc
     6181 ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac gaatacgtct
     6241 tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt caacgttcgt
     6301 catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta tctcaaaata
     6361 gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg atatgatttt
     6421 cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat tcctgtaaat
     6481 ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct tcacgatctc
     6541 cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc agtaacgcac
     6601 caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca tagacagaaa
     6661 aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc gtattgatcc
     6721 cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga atgatatcat
     6781 acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt acacggttca
     6841 agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg tccatgattc
     6901 cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc agctgaacgt
     6961 ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt gtactttttc
     7021 caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg tatttatgaa
     7081 gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga taaatcagat
     7141 gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca ttcaaacttt
     7201 tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa aattctcgcc
     7261 attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc tcctgcgttc
     7321 tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact taagagagat
     7381 tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt cttgacttgc
     7441 gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga tggaagacat
     7501 gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac aatgggcttt
     7561 tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt cacgtgaaac
     7621 agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt tacctgatga
     7681 aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt tggcatttgc
     7741 gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc tagcgcaagg
     7801 aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata tctatgaagc
     7861 agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa
     7921 agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg gtacactgac
     7981 aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt
     8041 tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa gtgtgaaaga
     8101 ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt
     8161 acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa
     8221 aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact
     8281 tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc
     8341 ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg
     8401 gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa
     8461 caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa
     8521 aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga
     8581 tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct
     8641 ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat
     8701 gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct
     8761 cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca
     8821 gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata
     8881 acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg
     8941 aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag
     9001 aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat
     9061 ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca
     9121 aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc
     9181 aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg
     9241 gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg
     9301 atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag
     9361 ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa
     9421 agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt
     9481 tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat
     9541 accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag
     9601 ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg
     9661 aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac
     9721 aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt
     9781 gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac
     9841 cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg
     9901 acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca
     9961 gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat
    10021 tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac
    10081 agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc
    10141 aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat
    10201 cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga
    10261 aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct
    10321 agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat
    10381 gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga
    10441 agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga
    10501 aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac
    10561 tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc
    10621 aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga
    10681 tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg
    10741 tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt
    10801 atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat
    10861 cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga
    10921 actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat
    10981 gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg
    11041 ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg
    11101 gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa
    11161 taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga
    11221 tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt
    11281 caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat
    11341 agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc
    11401 aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc
    11461 ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag
    11521 aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt
    11581 ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa
    11641 gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg
    11701 gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat
    11761 tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat
    11821 ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta
    11881 atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg
    11941 aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc
    12001 atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag
    12061 aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt
    12121 taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca
    12181 caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt
    12241 cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg
    12301 gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat
    12361 tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag
    12421 aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat
    12481 tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg
    12541 cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag
    12601 taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag
    12661 gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac
    12721 gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg
    12781 gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag
    12841 tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg
    12901 aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt
    12961 tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga
    13021 aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg
    13081 atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa
    13141 actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag
    13201 aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc
    13261 ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt
    13321 tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata
    13381 gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta
    13441 caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca
    13501 ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg
    13561 aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat
    13621 cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag
    13681 cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct
    13741 gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg
    13801 gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat
    13861 gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa
    13921 ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca
    13981 gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa
    14041 gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg
    14101 atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa
    14161 gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta
    14221 cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt
    14281 ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa
    14341 ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg
    14401 ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct
    14461 tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc
    14521 gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga
    14581 aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt
    14641 gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc
    14701 gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta
    14761 aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg
    14821 aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca
    14881 cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa
    14941 tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc
    15001 tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc
    15061 gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg
    15121 tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg
    15181 cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt
    15241 gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg
    15301 tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat
    15361 cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag
    15421 tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca
    15481 agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt
    15541 cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa
    15601 tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt
    15661 catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa
    15721 cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct
    15781 tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat
    15841 cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat
    15901 tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt
    15961 cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca
    16021 aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact
    16081 tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa
    16141 cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc
    16201 atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt
    16261 ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt
    16321 ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt
    16381 gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg
    16441 gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa
    16501 aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc
    16561 ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac
    16621 acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta
    16681 ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg
    16741 atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta
    16801 ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca
    16861 caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg
    16921 acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag
    16981 aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt
    17041 tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca
    17101 tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg
    17161 ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat
    17221 tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg
    17281 aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag
    17341 atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt
    17401 ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag
    17461 tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa
    17521 tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac
    17581 atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa
    17641 caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag
    17701 gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag
    17761 tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata
    17821 ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg
    17881 accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag
    17941 attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag
    18001 aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga
    18061 ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg
    18121 taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt
    18181 attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg
    18241 aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt
    18301 aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga
    18361 ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt
    18421 aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg
    18481 aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca
    18541 ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact
    18601 aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc
    18661 aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg
    18721 tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga
    18781 ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt
    18841 atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac
    18901 aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag
    18961 cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta tgcaagcgaa
    19021 tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt ttaaagcatt
    19081 ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt gtgcatattg
    19141 aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct ttgcgagcta
    19201 aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta cgcggtatgt
    19261 taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa
    19321 tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta aagaaattga
    19381 ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat ttagtactag
    19441 cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc
    19501 ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa gaaaagggct
    19561 atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat gatttgacag
    19621 agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca gcggctcttt
    19681 attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt agcgaccatt
    19741 tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat gaaatgatcg
    19801 aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct gccctgcatt
    19861 aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg ctggacttat
    19921 tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct gccgtttaac
    19981 tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact
    20041 tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact gtgacaaatc
    20101 gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc aactgttaag
    20161 tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc catgatgtct
    20221 tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc atgtgcagct
    20281 actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca gaatggcaaa
    20341 gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt gtctaacatt
    20401 gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa tggaggaaga
    20461 gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc agctgtttct
    20521 tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca tgatttgatt
    20581 tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt gattgcacga
    20641 gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat tttgcgtccg
    20701 ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca accagtaaat
    20761 tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt tggcatgttt
    20821 agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt tattgtacaa
    20881 ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa acaagtagag
    20941 aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta gatgcataat
    21001 tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac tgaaacaccg
    21061 cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg tcggattttt
    21121 ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt acgagcttca
    21181 tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc taggttttcc
    21241 attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc aagactaatc
    21301 aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg cgtagatttg
    21361 cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc agcgatcact
    21421 gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc atggaaaatt
    21481 tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac gtcatttatg
    21541 gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg attttctaac
    21601 tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt ttcgcaaatt
    21661 ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg acggattgcg
    21721 tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat gtttaggaaa
    21781 agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct ttctggctgt
    21841 tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt taagacttct
    21901 ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat atgaagtagc
    21961 tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag tttatcgact
    22021 aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac tattttctcg
    22081 aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat ttcatcagca
    22141 ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg tattcctaat
    22201 ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg taaaccttct
    22261 tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc ggtcccgaga
    22321 cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa ttgttttaaa
    22381 ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac tattttagcg
    22441 tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt caaaaaaaat
    22501 tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg ctttatctac
    22561 aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa ggatgcagac
    22621 attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac tagacgcaaa
    22681 tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt aaacctcgta
    22741 aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac agctttaaga
    22801 aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt gtgggtacaa
    22861 tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt ttgtcaaact
    22921 taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa cacagtgttg
    22981 tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat atcgttcgcg
    23041 gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa tacggaacta
    23101 aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta ctatatagaa
    23161 gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta aaggtcctgt
    23221 tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa ctcgcttgat
    23281 caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct ataatgcctt
    23341 tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac aagcaatgaa
    23401 aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa actaccaagt
    23461 accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg tagtaaacta
    23521 cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa tcatggatgc
    23581 tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa tggctgatgc
    23641 caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg gcttttataa
    23701 gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag gagaaacaag
    23761 gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca tggcccacat
    23821 cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta aaatccataa
    23881 aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag aacaagaacg
    23941 tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc gaatcaacat
    24001 catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat tgcgtgtact
    24061 tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa ctgaaacagt
    24121 ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca aaatggacaa
    24181 gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc aagcaaacgc
    24241 tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta tcgaccttgt
    24301 aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag aaacagaaat
    24361 tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg aagctgtagc
    24421 tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca ctgaagctga
    24481 gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag tactatgtgg
    24541 ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg attacttgcc
    24601 atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg aagaagtaga
    24661 acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta tgactgaccc
    24721 attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat caggttcata
    24781 cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac agatgcatgc
    24841 caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg ctgttggttt
    24901 gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta ttcttgaatc
    24961 catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa aagctgacca
    25021 agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt tccgtgctga
    25081 aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc acttagatat
    25141 catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg cgcctcaagt
    25201 atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg tacgtcagtc
    25261 tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg aagaaggtgc
    25321 tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat acattccagc
    25381 tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct atccattagt
    25441 agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa atgaaactgc
    25501 cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta atccagtaat
    25561 ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag gtgatgttat
    25621 gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg ctggcggcca
    25681 acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta caactcttcg
    25741 ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg aagatgtacc
    25801 aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat aatttcttaa
    25861 aaccggatag tcctgcaaag agattatcca aagagactat atttttaata tagtttcgtg
    25921 taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat atacctatca
    25981 gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa aagaaaaatt
    26041 tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc atggtaaaac
    26101 tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc aagctatggc
    26161 ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa tctcaacagc
    26221 tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc caggacacgc
    26281 ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta tcttggtagt
    26341 ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt ctcgtcaagt
    26401 tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg acgaagaatt
    26461 actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat tccctggtga
    26521 cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg cttcatacga
    26581 agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc cagaacgtga
    26641 caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg gacgtggtac
    26701 tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag ttgaagttgt
    26761 tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt tccgtaaatt
    26821 gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg ttgcacgtga
    26881 agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc atacaaaatt
    26941 ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc cattcttcac
    27001 taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg ttgaattacc
    27061 agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg aattaatcca
    27121 cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac gtacagttgg
    27181 tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt aaagcaacat
    27241 ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga gcttttttat
    27301 ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca cgaaataaaa
    27361 gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca agaaaaagcc
    27421 ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta gtgaattgca
    27481 cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca cgcccggacg
    27541 ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt ttcaaaatag
    27601 gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa gcgtatgaac
    27661 accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga actggagcta
    27721 gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc atccgtgcga
    27781 ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa cgtcttatcg
    27841 acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac ttaccatctg
    27901 gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt gtactcatga
    27961 ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa tctggtgaat
    28021 taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg aaaacaatgg
    28081 aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa gttttatcaa
    28141 acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc ttcattcgtg
    28201 aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca gtagatgttt
    28261 tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga ttccaagggg
    28321 ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt taccatcgtc
    28381 gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa cgtcttgctg
    28441 gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt gtcgatgcag
    28501 aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct ttaattacta
    28561 tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa ggaatgccga
    28621 atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt aacgaagaaa
    28681 tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg caacgtgcgt
    28741 cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc ggcggacgta
    28801 aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc tcaccacaat
    28861 ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac aaacttccta
    28921 aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct gaaaacaact
    28981 tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc aaacaagttc
    29041 ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt aacgactttg
    29101 ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat aatgtgagtg
    29161 tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt actcaaattg
    29221 aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga tcactgaaaa
    29281 atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc gcgcaaacaa
    29341 aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa acgtgaacat
    29401 cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata caaaaaaacg
    29461 tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat tcgaagctgc
    29521 tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc gattaaaaag
    29581 tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc tgaaatcaca
    29641 acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg acgtaacaac
    29701 aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata tcgcgtgatc
    29761 gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga atatgatcca
    29821 aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc ttacatctta
    29881 gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc agatatcaaa
    29941 gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca caacattgaa
    30001 atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc tcaagtactt
    30061 ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg tatgatctta
    30121 gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt aatcaacatc
    30181 ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg tagcgtaatg
    30241 aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg acgtaaagca
    30301 ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa aaaagctaaa
    30361 tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc atgtgtcgca
    30421 cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt tgtcgatgag
    30481 catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt tatcaaaact
    30541 tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc agtttatgat
    30601 ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa attaggtgaa
    30661 tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac gaaacgctaa
    30721 tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag caactgcaaa
    30781 aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca gaggtaaaag
    30841 tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg gaatcattga
    30901 aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag atgttgaaaa
    30961 cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt tccgtcctcg
    31021 tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg tagtcgtatc
    31081 agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga atgcgtgtag
    31141 gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct gagttcttac
    31201 acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct gctgtatcaa
    31261 cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca gctaaaccag
    31321 gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa ttaaacaaat
    31381 taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat ctagatgcaa
    31441 aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc cgtcgcgctc
    31501 aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa actcaagtat
    31561 ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa ggaactgttc
    31621 ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat acaacatacg
    31681 gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact aaaaaaaaca
    31741 ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc gtcgtgaatt
    31801 ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg gtgaatacgg
    31861 tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg ctcgtatcgc
    31921 aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc ctcacaaatc
    31981 ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac ctgaaggatg
    32041 ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc ctgaagaagt
    32101 agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta agatcgtaaa
    32161 acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa ttaaccactg
    32221 ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt agattccaat
    32281 tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa tcgattgcac
    32341 gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc attaagcgta
    32401 tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca gacaaaatgg
    32461 ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat ggcaaacgca
    32521 tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa gttggcgaca
    32581 tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt ttgttggaag
    32641 tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga ttgaatgcaa
    32701 aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa gtcgctgata
    32761 actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt cgtaaaactg
    32821 ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt ggggttgtca
    32881 aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct cgtcgtacag
    32941 acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat gataaaagcc
    33001 ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac aacttcatga
    33061 agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa aggaggtgcg
    33121 aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga aaagacaaaa
    33181 acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt gtcgaaggtg
    33241 taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt ggaattgttg
    33301 agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact ggtgtagctg
    33361 gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa aaaaccggtg
    33421 aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac cgcctaaaag
    33481 aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat agctctgtta
    33541 tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat gcggtatcaa
    33601 acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt caaaaaccaa
    33661 tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga atgccaattg
    33721 gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa ttggtttctg
    33781 tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt gacggtcgtg
    33841 gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat tatgatttag
    33901 tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct gatgaagaat
    33961 ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag gaggcgaact
    34021 acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt tcaacacaag
    34081 cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag ttccatcttt
    34141 gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg aagaaagcta
    34201 gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca tgacagatcc
    34261 aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg agtctttaga
    34321 agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg aaggtttcgt
    34381 tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt tcctaaaatt
    34441 tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac caggtttgcg
    34501 tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta tcgcaatcat
    34561 ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata tcggcggcga
    34621 agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt taagtgagcc
    34681 gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa gacggaaaca
    34741 acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca gatatcaaaa
    34801 tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa gaaatgaaaa
    34861 caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc agcgaaggtt
    34921 tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa ggatcaaaac
    34981 ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct ggagtaactg
    35041 ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa gaagttggcg
    35101 aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt aaaggtattc
    35161 gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa tcgtttagaa
    35221 aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac cttcaaaaaa
    35281 atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg cttgaaatag
    35341 tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa gaacaaaaca
    35401 cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga gcgcccacgc
    35461 ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga cgtagcgggt
    35521 gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac aaaaacagaa
    35581 gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg cattaaagta
    35641 gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt agctgaagct
    35701 gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg gtttatatcg
    35761 atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta acaaaagttg
    35821 ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac aaaaacggac
    35881 acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt aaagcaattg
    35941 aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc ccacacgaag
    36001 taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa ggttctgggg
    36061 ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca gatatcactt
    36121 ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt gaaggtttga
    36181 aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt gaagaattaa
    36241 tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac gcagtattat
    36301 cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa agattaacag
    36361 ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg tttctcacct
    36421 agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa ggaggtgcca
    36481 aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta cgtaaccgtg
    36541 ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa aaaggacaaa
    36601 aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca ccattgttcc
    36661 gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca gtcattaatc
    36721 ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct ttagttgaag
    36781 ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg gaactaaaca
    36841 agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca attgaagcgg
    36901 ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt ttaaagtaaa
    36961 agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc gcctaggtgc
    37021 acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt ctttgccatt
    37081 tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca ttttctcaat
    37141 gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa tggacatcgt
    37201 gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac tgaatcaagc
    37261 gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca ctgctggatt
    37321 caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt acgtaacgat
    37381 cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac agatcacaga
    37441 aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt ctagattgcc
    37501 tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt cagacatatg
    37561 gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg tgacattcgt
    37621 aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac gcgtagctgg
    37681 cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta ttccagttat
    37741 ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga gcggtagatt
    37801 cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata cagcacctgg
    37861 tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct ttgttcaagt
    37921 aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac caagcgtacg
    37981 ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa gtacagttgg
    38041 tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg tttgggatct
    38101 tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg tagctttaga
    38161 aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat tcattgagta
    38221 agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta ggagggaaaa
    38281 caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa gctgaaaaaa
    38341 tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc gcagcgatgc
    38401 aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt gcattagttc
    38461 ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat acagagaaag
    38521 gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg gacgcaatgc
    38581 taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt gaagaaatct
    38641 tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact taccataaaa
    38701 ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat gaattctatc
    38761 aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac atcaaaaata
    38821 gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt gatggtgctc
    38881 gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag cacgattgcc
    38941 cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat gatagaatat
    39001 gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt gaggtgggaa
    39061 ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga ggtactgtgc
    39121 gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt gccgaatgca
    39181 atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc aggtaaaatt
    39241 cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt gtctccatac
    39301 gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt aaaactacag
    39361 gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat tgtaaagtaa
    39421 ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat aaacaacgtc
    39481 aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata ttcctcgtga
    39541 taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta ctgctaaaaa
    39601 gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa cgaatgaaca
    39661 aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc ttcgtcgtga
    39721 agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa tccgtcatcg
    39781 ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc gtaaaggccc
    39841 agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt aaaacttcat
    39901 ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag caggtattgc
    39961 acatatccat tctacattca ataacactat catcatgatc actgatactc acggaaatgc
    40021 attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat caactccttt
    40081 tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg gattaaagac
    40141 tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc gttcattaca
    40201 agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc ataatggtgc
    40261 ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt ttatcagtaa
    40321 cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg attgagttcg
    40381 aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc atcgttgaac
    40441 ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt ttattatctt
    40501 ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat gaattctcta
    40561 ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa ggcttagcgc
    40621 tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga cctgctacag
    40681 taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa gatatgtaca
    40741 tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa cctggacgtg
    40801 gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt cttccagtcg
    40861 attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca cgtgttggac
    40921 accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca attgaaccgt
    40981 tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt tttgtgaact
    41041 taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca caaaaagaaa
    41101 aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac aattgtttga
    41161 aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct gaaatgatca
    41221 aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg cacgacttag
    41281 gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc aacgtgagtt
    41341 atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat ttaacaactg
    41401 atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa gttcgctcaa
    41461 ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt cgtcaagctg
    41521 cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac atcgtcattg
    41581 aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa cgtcaaggcg
    41641 gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca atggttgtct
    41701 tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt ttagagtgtt
    41761 atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag cgaatatcgc
    41821 ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc tgtcatgaca
    41881 gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa ttttgtcttt
    41941 ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga gccaattatt
    42001 gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc attgaaagat
    42061 gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa tggttcagga
    42121 aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg gatcgtgaaa
    42181 gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat ggttggaatg
    42241 atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga cgttgctttt
    42301 ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa agatgcactt
    42361 gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc aggagggcaa
    42421 aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat tattttagat
    42481 gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac gatcaagaag
    42541 atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga tgaagcagct
    42601 aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg tacaccaaaa
    42661 gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc atttcctgaa
    42721 aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata tatgacagaa
    42781 gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc acttatcagc
    42841 cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc aaagaaaaca
    42901 gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt cagcatttga
    42961 atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat attcagccgg
    43021 atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc tttcagtttc
    43081 cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt cctaaaaact
    43141 ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa caagttgggc
    43201 tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa atgcgtagag
    43261 tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat gaaccaacgg
    43321 caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt cttcataagg
    43381 aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc aattttgcag
    43441 attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg caagaggtct
    43501 ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc accgagtttg
    43561 cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca gctgaagaat
    43621 tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa attgatattt
    43681 ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc taaattatta
    43741 gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac gtaccttttg
    43801 ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa attttttatc
    43861 cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca ggtcttgttt
    43921 acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga atttggtgtg
    43981 atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc gactctactc
    44041 acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct tcgtccattg
    44101 aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc tcttcgtttt
    44161 gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc tcgcggtgtt
    44221 gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt gctgattcct
    44281 ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga agcaagaggc
    44341 tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa tagagacacg
    44401 atggttatca ttgcttttgt attattaact gtgctactga tctttttaag aggataaacg
    44461 ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag gatgcaagca
    44521 gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct ctttctttta
    44581 tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat aaccttttag
    44641 gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg acggaacaaa
    44701 ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag tagaaaaaac
    44761 attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa ggacagatgc
    44821 cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc gtccgttaga
    44881 acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa gacaagtaga
    44941 aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt atcaatttcg
    45001 agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt attttcctta
    45061 cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga ctcacgattt
    45121 cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga tctacgaggc
    45181 taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg gaaatggctt
    45241 cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca atggccgatt
    45301 gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag caggacctac
    45361 agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg ttatgataga
    45421 agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa aattttgcaa
    45481 aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa gaggtcgtga
    45541 gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc tcatctttga
    45601 tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg ttacctattt
    45661 tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg gacagtctga
    45721 acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc gtggttgttc
    45781 ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt accagacata
    45841 aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa atccttcagg
    45901 caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca agaatgcgtc
    45961 tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc ctttgtcata
    46021 aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag aagcttttct
    46081 gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg cttgataggc
    46141 tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg tcccataaaa
    46201 acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat agtattgacc
    46261 agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg cactagaact
    46321 ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat ttgcaggcac
    46381 atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt aagtgacgcc
    46441 agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg tatagaatgt
    46501 aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac tggtcaaatt
    46561 atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg ctagtggcaa
    46621 tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc ggaagccata
    46681 tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa acccatccat
    46741 cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac ggaaacctaa
    46801 tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac tgataccact
    46861 gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca agcggtaaaa
    46921 cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat aaggatcagt
    46981 atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca tactgttcgt
    47041 attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat gattgaatga
    47101 aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga aaaccactgt
    47161 caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga tgccccatgc
    47221 caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac catcgtcttg
    47281 ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga acagataggc
    47341 ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat tcaatggaac
    47401 aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt tatttttcaa
    47461 cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca gacctgcgta
    47521 aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat ttttcatgat
    47581 tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca gtgcctcgcc
    47641 atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc cgcctgccag
    47701 taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt ttgtcaatag
    47761 acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat aaaaactgga
    47821 taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat ccataacacg
    47881 atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca tgatgatctc
    47941 cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa catgccccca
    48001 gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac agccgacctt
    48061 aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa agaacaccag
    48121 tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca atcctagaga
    48181 agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga agctggcatg
    48241 aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc ttccaggata
    48301 gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga aactagctgc
    48361 tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat ttattttcca
    48421 ccaaatttct atgaagttgt attcagttta tcatatcct
//
LOCUS       DAJSBC010000015        47952 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSBC010000015 DAJSBC010000000
VERSION     DAJSBC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.015"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(38..1189)
                     /locus_tag="ONT65_000872"
     CDS             complement(38..1189)
                     /locus_tag="ONT65_000872"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR2328522.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ONT65_000873"
     CDS             complement(1202..1639)
                     /locus_tag="ONT65_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2328523.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ONT65_000874"
     CDS             complement(1654..3420)
                     /locus_tag="ONT65_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR2328524.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ONT65_000875"
     CDS             3684..4361
                     /locus_tag="ONT65_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328525.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ONT65_000876"
     CDS             4364..5491
                     /locus_tag="ONT65_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328526.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ONT65_000877"
     CDS             complement(5691..6131)
                     /locus_tag="ONT65_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2328527.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ONT65_000878"
     CDS             complement(6142..8175)
                     /locus_tag="ONT65_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2328528.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ONT65_000879"
     CDS             complement(8177..9880)
                     /locus_tag="ONT65_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2328529.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT65_000880"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT65_000880"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR2328530.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ONT65_000881"
     CDS             complement(10963..11988)
                     /locus_tag="ONT65_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2328531.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ONT65_000882"
     CDS             complement(12197..13066)
                     /locus_tag="ONT65_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR2328532.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ONT65_000883"
     CDS             complement(13525..15417)
                     /locus_tag="ONT65_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328533.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ONT65_000884"
     CDS             complement(15414..17141)
                     /locus_tag="ONT65_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328534.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ONT65_000885"
     CDS             complement(17157..17807)
                     /locus_tag="ONT65_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2328535.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ONT65_000886"
     CDS             18011..18307
                     /locus_tag="ONT65_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2328536.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ONT65_000887"
     CDS             18479..19099
                     /locus_tag="ONT65_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR2328537.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ONT65_000888"
     CDS             complement(19191..20357)
                     /locus_tag="ONT65_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2328538.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ONT65_000889"
     CDS             complement(20369..20722)
                     /locus_tag="ONT65_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2328539.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ONT65_000890"
     CDS             20850..21872
                     /locus_tag="ONT65_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR2328540.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ONT65_000891"
     CDS             21891..22052
                     /locus_tag="ONT65_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328541.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ONT65_000892"
     CDS             complement(22311..22745)
                     /locus_tag="ONT65_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328542.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ONT65_000893"
     CDS             complement(22855..24714)
                     /locus_tag="ONT65_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2328543.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="ONT65_000894"
     CDS             complement(24832..25992)
                     /locus_tag="ONT65_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR2328544.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT65_000895"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT65_000895"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR2328545.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ONT65_000896"
     CDS             complement(27739..28437)
                     /locus_tag="ONT65_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2328546.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ONT65_000897"
     CDS             complement(28394..30055)
                     /locus_tag="ONT65_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR2328547.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ONT65_000898"
     CDS             30273..31673
                     /locus_tag="ONT65_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR2328548.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ONT65_000899"
     CDS             complement(31925..33004)
                     /locus_tag="ONT65_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2328549.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ONT65_000900"
     CDS             complement(33090..34049)
                     /locus_tag="ONT65_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2328550.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ONT65_000901"
     CDS             complement(34061..35572)
                     /locus_tag="ONT65_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2328551.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ONT65_000902"
     CDS             complement(35594..36157)
                     /locus_tag="ONT65_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR2328552.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ONT65_000903"
     CDS             complement(36163..37026)
                     /locus_tag="ONT65_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2328553.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ONT65_000904"
     CDS             complement(37040..37936)
                     /locus_tag="ONT65_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2328554.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ONT65_000905"
     CDS             complement(38111..39400)
                     /locus_tag="ONT65_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2328555.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ONT65_000906"
     CDS             complement(39372..40889)
                     /locus_tag="ONT65_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2328556.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ONT65_000907"
     CDS             41087..41977
                     /locus_tag="ONT65_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR2328557.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT65_000908"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT65_000908"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR2328558.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ONT65_000909"
     CDS             complement(42886..44358)
                     /locus_tag="ONT65_000909"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR2328559.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="ONT65_000910"
     CDS             complement(44995..45357)
                     /locus_tag="ONT65_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2328560.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="ONT65_000911"
     CDS             45580..46332
                     /locus_tag="ONT65_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2328561.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="ONT65_000912"
     CDS             complement(46472..47395)
                     /locus_tag="ONT65_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2328562.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="ONT65_000913"
     CDS             complement(47410..47682)
                     /locus_tag="ONT65_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328563.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga ac
//
LOCUS       DAJSBC010000016        43706 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSBC010000016 DAJSBC010000000
VERSION     DAJSBC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.016"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            115..801
                     /locus_tag="ONT65_000914"
     CDS             115..801
                     /locus_tag="ONT65_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR2328564.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            803..1747
                     /locus_tag="ONT65_000915"
     CDS             803..1747
                     /locus_tag="ONT65_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR2328565.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1946..2590
                     /gene="pcp"
                     /locus_tag="ONT65_000916"
     CDS             1946..2590
                     /gene="pcp"
                     /locus_tag="ONT65_000916"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR2328566.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2669..3946
                     /locus_tag="ONT65_000917"
     CDS             2669..3946
                     /locus_tag="ONT65_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328567.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4130..4879)
                     /locus_tag="ONT65_000918"
     CDS             complement(4130..4879)
                     /locus_tag="ONT65_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR2328568.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            5209..5589
                     /locus_tag="ONT65_000919"
     CDS             5209..5589
                     /locus_tag="ONT65_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2328569.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            5685..6194
                     /locus_tag="ONT65_000920"
     CDS             5685..6194
                     /locus_tag="ONT65_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328570.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            6271..6450
                     /locus_tag="ONT65_000921"
     CDS             6271..6450
                     /locus_tag="ONT65_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328571.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            6475..7068
                     /locus_tag="ONT65_000922"
     CDS             6475..7068
                     /locus_tag="ONT65_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2328572.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(7115..7630)
                     /locus_tag="ONT65_000923"
     CDS             complement(7115..7630)
                     /locus_tag="ONT65_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328573.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(7656..8525)
                     /locus_tag="ONT65_000924"
     CDS             complement(7656..8525)
                     /locus_tag="ONT65_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2328574.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8651..9331
                     /locus_tag="ONT65_000925"
     CDS             8651..9331
                     /locus_tag="ONT65_000925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2328575.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9484..10464
                     /gene="pta"
                     /locus_tag="ONT65_000926"
     CDS             9484..10464
                     /gene="pta"
                     /locus_tag="ONT65_000926"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR2328576.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            10587..11060
                     /gene="tsaE"
                     /locus_tag="ONT65_000927"
     CDS             10587..11060
                     /gene="tsaE"
                     /locus_tag="ONT65_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR2328577.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            11053..11577
                     /locus_tag="ONT65_000928"
     CDS             11053..11577
                     /locus_tag="ONT65_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2328578.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(12067..12600)
                     /locus_tag="ONT65_000929"
     CDS             complement(12067..12600)
                     /locus_tag="ONT65_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR2328579.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(13464..14216)
                     /gene="xth"
                     /locus_tag="ONT65_000930"
     CDS             complement(13464..14216)
                     /gene="xth"
                     /locus_tag="ONT65_000930"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR2328580.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            14329..15249
                     /locus_tag="ONT65_000931"
     CDS             14329..15249
                     /locus_tag="ONT65_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2328581.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            15322..16257
                     /gene="murB"
                     /locus_tag="ONT65_000932"
     CDS             15322..16257
                     /gene="murB"
                     /locus_tag="ONT65_000932"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR2328582.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            16555..18033
                     /locus_tag="ONT65_000933"
     CDS             16555..18033
                     /locus_tag="ONT65_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328583.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            18310..20370
                     /locus_tag="ONT65_000934"
     CDS             18310..20370
                     /locus_tag="ONT65_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR2328584.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            20453..21022
                     /locus_tag="ONT65_000935"
     CDS             20453..21022
                     /locus_tag="ONT65_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2328585.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(21197..22003)
                     /locus_tag="ONT65_000936"
     CDS             complement(21197..22003)
                     /locus_tag="ONT65_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328586.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            22141..23655
                     /locus_tag="ONT65_000937"
     CDS             22141..23655
                     /locus_tag="ONT65_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR2328587.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(24058..25041)
                     /locus_tag="ONT65_000938"
     CDS             complement(24058..25041)
                     /locus_tag="ONT65_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR2328588.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(25046..25693)
                     /locus_tag="ONT65_000939"
     CDS             complement(25046..25693)
                     /locus_tag="ONT65_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2328589.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(25778..26410)
                     /locus_tag="ONT65_000940"
     CDS             complement(25778..26410)
                     /locus_tag="ONT65_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2328590.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(26426..26977)
                     /gene="coaC"
                     /locus_tag="ONT65_000941"
     CDS             complement(26426..26977)
                     /gene="coaC"
                     /locus_tag="ONT65_000941"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR2328591.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(26974..27711)
                     /locus_tag="ONT65_000942"
     CDS             complement(26974..27711)
                     /locus_tag="ONT65_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR2328592.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(27708..28697)
                     /locus_tag="ONT65_000943"
     CDS             complement(27708..28697)
                     /locus_tag="ONT65_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2328593.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            28955..29497
                     /locus_tag="ONT65_000944"
     CDS             28955..29497
                     /locus_tag="ONT65_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328594.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            29514..30599
                     /locus_tag="ONT65_000945"
     CDS             29514..30599
                     /locus_tag="ONT65_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328595.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            30611..31417
                     /locus_tag="ONT65_000946"
     CDS             30611..31417
                     /locus_tag="ONT65_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328596.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            31418..32245
                     /locus_tag="ONT65_000947"
     CDS             31418..32245
                     /locus_tag="ONT65_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328597.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            32242..33315
                     /locus_tag="ONT65_000948"
     CDS             32242..33315
                     /locus_tag="ONT65_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2328598.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            33743..36439
                     /locus_tag="ONT65_000949"
     CDS             33743..36439
                     /locus_tag="ONT65_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2328599.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            36516..37004
                     /locus_tag="ONT65_000950"
     CDS             36516..37004
                     /locus_tag="ONT65_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2328600.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            37016..37510
                     /locus_tag="ONT65_000951"
     CDS             37016..37510
                     /locus_tag="ONT65_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2328601.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            37531..38340
                     /locus_tag="ONT65_000952"
     CDS             37531..38340
                     /locus_tag="ONT65_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2328602.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            38337..39158
                     /locus_tag="ONT65_000953"
     CDS             38337..39158
                     /locus_tag="ONT65_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2328603.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            39209..41308
                     /locus_tag="ONT65_000954"
     CDS             39209..41308
                     /locus_tag="ONT65_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR2328604.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            41305..42423
                     /locus_tag="ONT65_000955"
     CDS             41305..42423
                     /locus_tag="ONT65_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2328605.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            42462..43340
                     /locus_tag="ONT65_000956"
     CDS             42462..43340
                     /locus_tag="ONT65_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2328606.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            43531..43683
                     /locus_tag="ONT65_000957"
     CDS             43531..43683
                     /locus_tag="ONT65_000957"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2328607.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVLVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        14109 a         7580 c         9107 g        12910 t
ORIGIN      
        1 aaaaataacg aaaaatattt aaaaatagtg tctatttcgt gagaaaacaa ctatattttt
       61 tatgtgtttt gctttataat taaaaaaata ttgtcatttt atggaggaaa atatatggaa
      121 aattatctta gccttttagg cgtcttgatt gttattctcg ggtttgcatt taaattagac
      181 tcgatattga tcgtaatggt tgctttagtt gttacagctt tgactggcgg tcttggcatt
      241 caaggaatgt tagaactttt aggtacaagt tttgtaaaca atcgcgggat ggcgattttt
      301 atcatcatta tgttggcaac aggaacgctt gaaagaaatg gtctgaaaga atcagcggct
      361 tccttgatca agcgattcaa gaaagtatct gctggtatga ttgtagatat ttatgggatc
      421 tttcgaatga tctttgcggc ttttaacgtg agttttggtg gagttgctgg atttgttcgt
      481 ccaatcattt taccgatggc attggggacc atcgaatcaa aagggttaga agtccatcct
      541 aagcatgaag aagaactaaa aggtatagct tcagctatgg aaaatgtctg ctggttcttt
      601 ggccaagtac tattcatcgg aggagcaggg gcgttgctag tccaatcaac actgaaagat
      661 ctaggatatg acgtaacatt gggacagctt gctttagtgg aagttccagt ggctatcacg
      721 gctttagttg tggcaagtgt ctacttctat cttaaagaaa agaaattagc gaagaaatat
      781 tatccaggaa aggggcaata agatggactt ttttacaaat cctgatattt tattaggcga
      841 taaattactg gagatccttt atatcgtgat gggactcgta ttgatttaca ctggagtaag
      901 aaatttaaca gataaaacca atcctcatcg ttatgggtca gcctttttct ggacggtctt
      961 aggggtagtt attgcaggag gaagatggct tccggcaatc gtgaacggtg gcttgatctt
     1021 tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa ggagaaagcc ggcttccatc
     1081 aaaagaatat atggaaaaaa tgtcagacaa actaggaatg aaaattttta ttccagcttt
     1141 gagtatcggt gtctttgcga tcttgtttgc tctatttacc aatttaggtg ctttagtcgg
     1201 cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg ttcttttctc gagataatcg
     1261 accaagtaca tttttagatg atgcagcaga tatgctgggc acagtagggc cgttaagcat
     1321 gttgccaatg cttttagctt ctctaggtgc ggtatttaca agcgctggag ttgggacagt
     1381 gatttccaat gcagtaggta cgatcgttcc agaaggaaat gtgaacatcg gcatcatcat
     1441 ttacgctctt ggcatggtga tatttacggt aatcatgggg aatgcatttg cggccatcac
     1501 cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt catggagcga atccagcgtt
     1561 aataggaatg gttgcattga cttgcggttt ttgtgggacc ctgctgacac cgatggctgc
     1621 taacttcaat atcgtgcctg tagcgatgct ggagatgaaa gacaaatacg gagtgatcaa
     1681 aaaccagttg tttattgcgt tatttatgct agttttccaa attgcttata tgattctatt
     1741 taaataatag aagagagggg ttgtgacaga ataattgtcg caacctcttc taacattgta
     1801 gtggaacact atttttttat aagaatatat taagtttgat ctgttatgtt atttaaacta
     1861 ttgtttcctt tatttacgct gttcaaggaa aatggtatga taaaagataa ccatatgtta
     1921 aatcaatcgg acggaggaac aacagatgaa agtactagta acaggttttg atccatttgg
     1981 tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa atgccagatg aaatcagcgg
     2041 tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa aaaagcagtc aagtagtcaa
     2101 agaagccatc caacagcacc aaccagatgt cgtgatctgt gtcggacaag caggtgggcg
     2161 gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca gaagcaagaa tccctgataa
     2221 tgaaggaaat cagccattcg atacggcttt agaagaaaac ggtccagccg cttactttac
     2281 ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat catggtctac cagcgtacat
     2341 ctcttataca gcaggaacat ttgtctgcaa tgatatcatg tatcgtttgc ttcatttgat
     2401 cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac gtgccattct cacccgatca
     2461 agtgatcgat cgtccagtgg gtacgccttc tatgtcttta gaagacattg cttcttcttt
     2521 gacttatgca gttgaagcag cagtagaaaa taaagaagat atttccggaa atgccggaac
     2581 aactcactaa tttagcaaga aacactggct ggttgctaac gttatgttag tagccagctt
     2641 tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg caatccgtct ttgaagaatg
     2701 gtttccaaaa gaaatcagca aaagctatcc catcaaaatt tctaaacaat acacaagcag
     2761 tcaacgttgg gagatctatg caaaattaac gaaaaaacag cgggaactag tcgataagca
     2821 ccgccgatat ctaatcagct ctcgttttat ggaagaacat tatttagctg caacagattg
     2881 ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca aaacgaagcc agcaaaaatt
     2941 gtattgtgag tgtggtagag aattaaaagt gcaatacatc gttaaatcgc caaaaaccgg
     3001 aaagatccta aaactaggca tcaaccactt tgccgatcac ctgcatgtct ctccgacagt
     3061 ggcagcttcg atccatcagg gaatgacaaa agttgatctg gccttagacg aattattatg
     3121 gcttaagcag aagaatatcg attttcctga aggactatgg caaaaatact gttttgtctt
     3181 gtaccaaaat cgtcggatga aacagccata tttaccggat ataaaattag ctcagcggct
     3241 cgcagaattt cgccaagtag aaatgccgat ttacatagcg gattatcaag cattggaaaa
     3301 tgaaatcaag aaaataagtg aacacatcaa tgggcagtca aaaaagcgac agataaaaaa
     3361 agaactattt gacgactttg cagaagaatt agtaaaggat gtagaagaat tcttgatcaa
     3421 ctatcgcgca tttttacgta aagattggca atccatcgta tatgaagaag tgccagtaca
     3481 tcctaatgct tattttgaaa catttatttc cgtgttgaga aagacaaaac gtcaacgaac
     3541 accagaagtc actgctcaga tggaatattt tgcgaaaaac caacgattca tccaaccaaa
     3601 aatctatcta tttatttgga agcaatattg tcgctatggt tttacagaag ggttcttcga
     3661 cagcattcct cggatagtca gaaatggttt tttaaaagta ttgcgtaaag aacgagaagc
     3721 tatacaatct gctgataaga aagaccgtac agtctcaaaa gaaaaatggc aactagtagt
     3781 gaaagatatc caatctggaa atgtgcaaga gacaatcgac aaatggaaag gaaaacatta
     3841 tcgttttact gaagctcaga aacaagcgtt ggaatattat cagaagttag aagaatcact
     3901 gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta ctataaaaaa tagaggctgg
     3961 gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct tttcaaattt
     4021 ttgtgaattt cagcttattt ccgaaggagt tgcttctgat tccgccgttt atacgtgttt
     4081 agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tcagtttttt tatgcgtgtt
     4141 gttgtgcgtt gtagtattct tcttccattt tttgcatacg ttcacgttct ttttggttat
     4201 accaaggtga aacggtaaat gcaaggacat cattcagcaa gtaaacagag ctgttcacga
     4261 acatcgccaa acttgcagat ccatgcgtaa atgaaatata ccaaaggacc atttgagcaa
     4321 gccctgaagc taaccaccag taatattggt tgttgtaacg caagaaactg atgataccag
     4381 cagtcaagct aatagaaaaa gcaatggcat ctacccacgg ccgaggatca tctgtataac
     4441 gaccaatcag gaatcctgaa acaagataga caaggaaagt actgatgatt gcgataaacc
     4501 agctttttcc agtgaatttt cggatttttg aagccatatt atgattccat tctttactta
     4561 acaatacggg gatatccaat gtgatcatat aagcaatctg ttcaccgata cttaaatagt
     4621 tctttgctga aaacccaacg ataataaaac aaatcgcaga caaaatacct aaaataccgt
     4681 tgactgattt tgccgcattg atggataaaa tacacaatac accaagcgtg gtaccgataa
     4741 atgtaataac ggacagccaa gtgatgggac tattgacaag agtcatgacc tggcagccaa
     4801 gactgaaaaa gaaaagataa taattttgtt gcggccagcc ttttagctga tgagctaaaa
     4861 actgaaaata tgacttcatg tatgaggact ccttaagaca ctaaatgtag tgtgttctgt
     4921 atttactaac acaatctatt gtatgctttt cttgaaaaaa aactagtctt tcaattataa
     4981 ataatttgtg ttatgataat tttctgtttg ttttcttgaa aatataattt cttactttca
     5041 tttattatat tgggaacagg aagagtgtct tggaggacct actttctgtt ttttttgcct
     5101 tttttcttat aaaaaggaaa aacagctatt tttttcaatc tgacacattt acaatggaac
     5161 tggagattaa tatagatccg agcttaaacg aagaatagga gtgctcatat gaaccttaca
     5221 acgatccatc atatagccat cattgtttca gattatcaga aatcacgtga tttttatgtt
     5281 aataaattag gatttgaagt aatcagagaa aattaccgca aagaacgaga tgactataaa
     5341 ttagatctga aactaggaag tgctgaattg gagatttttg gaattgctaa tgcgccacaa
     5401 cgaccaaact atccagaagc ttgtggcttg cgtcatcttg ctttcaaagt agacgatatt
     5461 gaagaaacaa ttgcagaatt gcagtcctta gggatcgaat cagaaccgat tagagaagat
     5521 acctttacag gaaaaaaaat gacgtttttc tttgatccag acggattgcc attagaactg
     5581 cacgaataaa gataacaaaa ttgtcacttt tttttcgttt cattcgattt ttcattcgtt
     5641 catttcttga tattctttaa aaaaaagaaa caggagaatg aagaatgaaa aaaatgatgt
     5701 ttggtgttgc tgccctcatc gtaataggga ttaccggtat gacgggactg ctcaatgccg
     5761 cttttcacat ggatgatatt agtgaccagg aatatcggat ctcattgaaa aaagcagcag
     5821 caatcgtaaa aaacgaaaca aaagaacata acatatctgc tattcatttt gcagttgaaa
     5881 aagatttgga gaatcctacg aatgcaacat atgaatacca gtttttttgt gaaggacaag
     5941 tcatagcagt caatccatcc actgggaaaa caaccgtacg aaatgacacg gaaaaggccg
     6001 cagaacatct ttttgatata gaaaaaacgg aagagttcaa acaaccgcaa gcagcgatga
     6061 aggaagcagt cagctactac gggaaaaaag caaaagtgaa agagtggaca cttctagtca
     6121 aagatgaacg tccttattat aaagtccgat taatagatgg agaacaggac cgtcaggtat
     6181 ggatatctgc atagaagaag gatattgctt ctctttttta caatataaga gtaagctaag
     6241 agagtaaaga tgaaaggaag ttgagataac atggaaaaaa tgaaatgccc taattgcgga
     6301 aaaaaatttg catacgaaga agtcaataat gtagtcgaac ataatgataa agaaatgcca
     6361 gtcgtctgcc cttattgccg aactgaagcg gcaagaatcg ttactcatgg ttatttcatt
     6421 actgaaaaga ttgaagattt tttaaaataa cgattttgcg atttcttttt ctgcatgtta
     6481 gaatggagaa aaggagagga aactatgatt cgttttgcga gaaaagaaga tgctccagaa
     6541 atcgccccat tgatcttagt gattctaaaa gatatggaac tgccattttt gaagcagaca
     6601 ggggaacaaa agactttaga gattttagaa gaagcaatca ctgatccaaa ttatcgttac
     6661 agttattcac gaggtatcgt agaagaaata gacggtaaag ttgcaggagt tgcttacggt
     6721 tatacagatg aagaagaatc aatcatcgat cagccactgg tacctatctt agagaaatat
     6781 cagctagatg cgtctacacg tttgttcatt gataaagaaa cgtttcccaa cgaatggtat
     6841 ttggactcta tcagtgtatc agaagatttt agaggacaag gcatcggttc gcgattatta
     6901 gaagcattac ctaaattggc gaagaaagct aatcgctccg tcatcggtct aagtgtggat
     6961 gaaaaaaatc caaaggctaa aaaattatac gaacgccatg acttcaaagt tgtcggtcag
     7021 cggatgatca gtggtcatct ttatgatcat atgcaaaaga atatctaaaa agatgtacct
     7081 gaaatgcgta tttccgcatt tcaggtacat ctttttaact taagttaagt cggtaagaaa
     7141 caggcttatc gcgacttttg tctttacggt agagccagta atctaaaacc gcagcaatac
     7201 aaatacaaac tggagcatct tcatcatttg agacatccaa tacatagtaa tctcctgaaa
     7261 aaagtgttgc tttatccatt ttcatgattt gctgattgaa atgatggatt tgatattgat
     7321 gattatagat atcaccttgg actgtccagt gaagtctacg aatataataa aagtcaccgg
     7381 gccaattgaa tattttctgc atggttccca ccttttcgaa ctctttatac agctcaaaac
     7441 gcgtaccgaa tgtcaggctt atctgcttga tgtgagcaac gagatcgcca ttcatggcat
     7501 aaagagacaa tgcatcgccc tttgttcccc agcgaccaac catcaaaaac agggaatgtc
     7561 ctttttcgtt tttgacaatt gtccgagttg cacgtccaag ctgcttttcc tgaatgaaaa
     7621 attctgacat ctatatccct cttttctaga taaaactaca agtcttctgc aatcgcgcgc
     7681 aatatggctt ttcctacgcc agattcaagg tttgtgtccg ttgtatattt ggcataatct
     7741 tttacttcga tttctgcatt tcccattgcg aaactgactc ctgctacttg cagcatgctg
     7801 acatcattga agttatcgcc aatcgtcatg acttcttcta aagaaatccc acgctcgtga
     7861 gcgacatggg caacagcaat tcctttttga gcgtttttat ggttcacttc gatattgttt
     7921 tgaccagagg aagtaacggc tagatcgcct aaaagagaaa tctgctttcc aactggacct
     7981 aatacagctg gcccttctgt atgaaaacaa ataattttta aaacttccag attcttttct
     8041 tccaaaatag cacgaatact gtccacgtat gtgatgtgca ataactccag cttagctgag
     8101 gccatggcaa tcgccatttt atacgtaagg tgaggtaaat gggtagccac catggaagca
     8161 aaactttcaa ttcgtttcgc ttgattttca gaataaagac catcgttggt tgctacttca
     8221 tagtaaatac cgttagcatc tagaatatct aaaacagaga ttgcttgatt atttggaatc
     8281 gacacggtaa acaatgaatt tccttcttta tcaaatacct gtgccccgtt caaagtgatc
     8341 atcgcacaat cgattcctac ctcatctaaa gctgcgcgag cttcttgata gtttcgacca
     8401 gtagcgacca tgaactcaac accggcatca tttgcaaaac gtatcgcctc ggcattttca
     8461 ggagaaacac tcatatgggc atctaataat gtgccgtcca tatctgatgc aattaatttg
     8521 atcataatga ttatcgttcc tttctgcttc ttgttcctag tttaccatat taatcaagtg
     8581 ctgagggtga agattgtccc ttgtgattga agaaaatgtg agttattctt tttattagga
     8641 ggtggagttt ttgaaagaaa tcattcataa cagttggcaa gaagttttgt cctcagaatt
     8701 ttcaaaagat tattatctgc atctaagaga atttttaaaa aaagaatatg cgtcccaaaa
     8761 gatccatcca gatatgtatc atatctatga agcattggaa ttgactcctt atgaagaagt
     8821 taaagtggtg attttaggac aagatcctta tcatggagaa aatcaagctc atggcttatc
     8881 tttttctgtg caacctggcg taaagatccc gccatcgtta agaaatatct ataaagagct
     8941 ttatgatgat cttggaatcg cacctgttca acatggaaat ctagtgtcat gggctaaaca
     9001 gggagtcctt ttactcaaca ccgttcttac cgttcgggaa gggcaagcgt attctcatcg
     9061 cgggaaaggt tgggaaagat taacagacac gatcattgaa aaattaaatg aacgggaaaa
     9121 accaatcgtg tttatcttgt ggggaaaacc ggcacaagaa aaaatcaaaa tgatcgataa
     9181 gagtcgccat atcatcataa catctccaca tcccagtccg ttatctgcaa gtcgtggctt
     9241 ttttggttcc aaacccttct cgaaaacgaa tgatgcgctc cttgcgttag gagaagaacc
     9301 gattgattgg caactgcctg aaacagtgta ataaaacagc gctttcctgt tatagtgcag
     9361 ttcgctttgt atgtgaaaaa cttaattttt ccagcatttt tgactgatac agataaaata
     9421 aactgttctt tttttctgga atagtgtatg ataatacata taaaaacgtc tggaggtcat
     9481 cttgtggaat tgtttgatag tttaagattt aaaattgttc gccgtgggat caaaatcgtt
     9541 ttcccggaag ctactgatgc tcgtatttta ggcgcagctg cccggttgaa agcagaagaa
     9601 cttgtggaac ccattttagt agggaacgaa gaagaaataa gaaatgcagc gcatgccaga
     9661 ggaatcaaaa catctagctt tacgatcatc agccctgata attacgataa gtgggatgaa
     9721 atggtggata cttttgttga acgtagaaat ggaaaggcat ccaaagaaca agcagaaaag
     9781 attttaaaag atgtgaatta ctttggtacg atgcttacgt atatggggat tgcagacgga
     9841 atggtctctg gagcgatcca ttcgactggc gacactgttc gcccagcact tcagatcatc
     9901 aaaacaaagc ctggcatcag tcggacaagc ggagcgatga tcatggtacg tggaagagat
     9961 caagaaaaat atgtcttttc ggattgtgcg atcaacgtga atccaactgc gcaagaattg
    10021 gctgaaatcg ccgtagattc tgcaaaaaca gcagaattgt tcgatatcga accaaaagta
    10081 gcaatgatga gcttctcgac aaaaggctca gcgaaatcgc cagaagtaga caaagtagtt
    10141 gaagcaacta aaatcgctaa aaaactagcg ccacaataca tgatcgacgg cgaattgcaa
    10201 tttgatgctt cttatgttcc ttcagttgca caactgaaag cgccagattc agaagtagct
    10261 ggtcaagcaa cagtctttgt atttccagaa ttgcagtcag gaaatatcgg ctataaaatt
    10321 gctcagcgtt tcggtaactt tgaagcaatc ggaccaattc tacaaggctt gaacaaaccg
    10381 gtttctgatt tgtctcgtgg agcaaatgaa gaagatgtat ataaattatc gattatcaca
    10441 gcagcacaaa cattgattga ttaacaaata cacgcgaact agccaaaaac agcagctgct
    10501 gttcttgact agttcatttt ttctttaaaa aaacttcatt acgcgatata ctggcaaaaa
    10561 gaaagaagag cgggagaaga tgaaaaatga tcgaactatc aggattaaca atgacagaac
    10621 aattcgcaga ggcaattggt cattccgcaa tgccaggtga caatctgata ttgactggtg
    10681 atctaggtgc gggtaaaaca acgttgacga aaggaattgc ccaaggatta gggataacgc
    10741 aaatgatcaa aagtccgaca tacacgatta ttagagaata tagtcaaggt cggattccgt
    10801 tgtatcatat ggatatttat cgggtagcag ctagcggagc agatttaggg ttagatgaat
    10861 attttgaagg tgatgggctt tccgttgttg aatggggaaa tctattagaa gaagcactgc
    10921 cggaggatta tttggaactg attttagaaa aaagtgatac agatttagaa aagcggtatg
    10981 tgaaactaat ctcatacggg gagcaaagtg aaaccttcaa acagcgaatt ctagaaaaat
    11041 gggctgaaaa tcatgagtga agaagttggg atcatcttgc gggaagcagt gccaggagat
    11101 gcaaaagaca ttcttttgat gatgggacaa gttaataagg agacagaatt tttagttttg
    11161 gatgaagcag aactcctgtt accaccagaa accttagaag aagagttgga ttatatatac
    11221 gaaagcaata ataatttatt gctgcttgca atctatgaag ggacgattat tggaacagca
    11281 tctgtcaagg cagacagtca atttcgtttg tctcacgtgg gagaagtcgg tatcagtatt
    11341 ctgcaggaat attggggaat gggactaggc actcttatgc tagaagaaat catcagttgg
    11401 gcgaaggaaa tgggtgtact ttttcgattg gagcttgacg ttcaagttcg taatgaaaga
    11461 gccgtacacc tttaccgaaa aatgggcttc caaatcgaag cggtcatgcc tagaggggca
    11521 agaacagatc taggtgaatt tttagatgta tataaaatga gttatttgat tgaatagaag
    11581 aaataggaaa agagtgagaa actgtcatcc ttttgagaag gcagaaaaac acacgaaggg
    11641 actgtgacaa aagtcgtgtc gcagtccatt ttttttgaat aaacggtgtt caatcagctg
    11701 accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct
    11761 ctaatactca ggtcaaaacg ctgatttcac aacctcttgg taaacggtgt ccaaaagctt
    11821 gcatctgcgg taataagtcc aaccaaatga aaaatatgag aagctatttt tgtttggctg
    11881 ttcttattac ttgatgcaga acagcttttt cacaacctct ccaataaacg gtgttctcct
    11941 gccagctcct cggtcaccag tcacaatatt taataaacat gagaagctat tttttgaaat
    12001 tgctccttgt gactcggagt gaaacggcag gctcacgacc tctttgtgtg tttcataagt
    12061 attcttttac tttatagtca caagtttttt caatggatct gtcccgaatt ttttttcttg
    12121 ataaagcagg atttccgcac atgcacgact atcttccaat gcatcatggt gatgatctaa
    12181 ttggatgttc aaattttcgc acacagtgtt caatcgatga tttggaaatt caggaaacag
    12241 gcgacggcta cttttcactg tacataaaga aagataatta ggctgttcaa gatgataata
    12301 atctaagcat cccgctaaga cctttgtatc aaaagcagca ttatgtgcaa cgactaaacg
    12361 atttttttga aaatatggtt ggatagtttg ccagacttct gggaatttcg gcgcattgcg
    12421 tacgtcttct tgatggattc cgtgaatttg gacatttcgc cagaaaaaag gagtctcagg
    12481 ctgtatcaat gtatagaatt catcaacgat ctggctattt tttaccatga ctagcgcaag
    12541 agaacaagca ctataaggct gatgattggc agtttcaaaa tccatggcaa caaaattcat
    12601 ttctgtaaaa cctccttggg aaattactgt aacatacttt tgaaaaaagc aaataaattt
    12661 aacaaaaaac atagaatttt ttatgtaata tttgttgaga aaagctagtt tttgaactca
    12721 ttccttgtga tttggagtta gatggggatc atgacttctc ggtattaagt caaatttatg
    12781 caaagttgtg aggaaccatt ttcaaaaaaa tttcttacga ctcgaaagta ggcgactttt
    12841 tcacaacttc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat
    12901 agcaaaatat gaggaactat tttgccattt gtttctctaa tactcaggtc aggacgctga
    12961 tttcacaact tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
    13021 aaacgaaaaa tatgaggaac tatttttgtt tggctgttct tattacttga tgcagtgcag
    13081 ctttttcaca acctcttaat aaacggtgtt caaaagcttg catctgcggt aataagccca
    13141 accaaacgaa aaatatgagg aactattttt gtttggctgt tcttattact tgatgcagtg
    13201 cagctttttc acaacctctt aataaacggt gttcaaaagc ttgtatctgc ggtaataagc
    13261 ccaaccaaac gaaaaatatg aggaactatt tttgtttggc tgttcttatt acttgatgca
    13321 ggacagcttt ttcacaatct ctcgataaac agtgttcaaa agccagtttc tcggtattat
    13381 gccaaattta tgcaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac
    13441 taaacgactt tttcacaacc tctttatttc aaatctaatt ctactggaca atgatcactg
    13501 cccataatgt ccgtatggat ctttgcatca gctagtcctg gctctaaatc tttgctcgtc
    13561 aaaaagtaat cgatccgcca accagcattg tttttccttg aattgaatcg gtagttccac
    13621 caagagtaga caccttcctg ggttggataa aaataacgga atgtatcgat aaacccgtta
    13681 tccaacagtc gagaaagagc cgctctttct tctggagtga atccagcatt tttttgattc
    13741 gttttccagt ttttcaaatc aatcttttga tgagcgacat ttagatcccc acacacgatg
    13801 actggttttt gtttttttag attttccaaa taattataaa aggcttcttc ccattctaat
    13861 cggtaatcta aacgttttaa ttcgctttga gaatttggcg tgtaacaggt tacaagaaag
    13921 aaatcggaat attccaaagt aatcagacgg ccttctgtat catggatatc gatacccatt
    13981 ccgtaagtgg cattcaatgc tggtttttta gcaaaaatcg ctgtgccgga atagcctttt
    14041 cgttccgcgt aattccaata ttgataataa cctggaagat ctaaatcaat ttgtcctgct
    14101 tggagtttcg tttcctgcag acagaaaaaa tcggcgtcta actcatgaaa aacttctaaa
    14161 aaatttttat ttacgatggc acgaagcccg tttacgttcc aagagataaa tttcaataaa
    14221 agcacactcc ttcgaattaa tttccttacc tttgcgatac tattgtacct cattttagta
    14281 aaaaatggtg taagatgaaa gtaactaaat gatccaggag gaaagtagat gaagattggt
    14341 gtaatcggtg tagggaatat cgcagaaaaa gcatatttac caacttatgc gaaaaaacaa
    14401 ggaacacttg atttttattt tgcaacaaga aataaagaaa caaaagaaag aattcaaaaa
    14461 gagtacgggt tccaacatct atatgaaacg atcgatgagc ttttagaaga aaaaatcgaa
    14521 gcatgtatga tccacgcagc aacaaaagta cattatgaat tagccaaaaa atgtctagaa
    14581 aatggtatcc atgtttatat tgacaaacca ctgagtgtcc atctaaatga agttcgtgaa
    14641 ttgcaagaat tagctgataa gaaccaaata attttgatga tcggtttcaa ccggcgcttt
    14701 gctcctatgg tagaagaatt gaaacgtatc ccagataagc ggttgatcca cctgcaaaaa
    14761 aatcgaatag cggcaaaaga aacgacagag ttcgtccttt atgatttgtt tttacatctt
    14821 attgatacag ctgtgtattt attagatgaa cccattttga gaacaaaata ccagataaga
    14881 gaaacaaagg aatttttgga atatgccatt ttacaattag aaacacaaaa tcaaacggct
    14941 attttaacga tggatttagc aagtggcgct aatatagaaa aatatcaagt aacaagtaaa
    15001 aatggtactt atgaagtaaa cgatctgacc cagcttgtca ttcaagaggc tgatggaaaa
    15061 aaagtgaaag aatttggcga ctggacaaac acgctagtca aacgcgggtt tgagccaatg
    15121 gtagaggcct tcttttctca gatccgttta ggtaaatcgg aaacagaatt gcttaaacaa
    15181 aaagaagttg taagaagtca tgaattatgt gaggaagtgc ttaaagatca ctaccgtcat
    15241 caactttgaa caagactggc aagattgtat taagcgtgat agaataatga ggacgtttga
    15301 gaaaagaaag gaaaatcctc agtgaataaa gaagaaatag ttgaaagctt agccacgatt
    15361 actatattgt ttgatgaacc attgatgaac tatacgttta cgaaaacagg tggaccagct
    15421 gatgtccttg cattcccgaa aaaacaagaa gaagtaaagc aaatcatcga ttattgccga
    15481 atccatgata taccttggat ggtattagga aatgcaagca atctgatcgt tcaagatggc
    15541 gggattcgcg gtgtagtcat catgctgact gaaatgaaac agattcatgt caaaggaacg
    15601 atggtcattg cggaagcagg tgcctcactg attgatacga cttatgcagc tttggcagaa
    15661 tcattgactg gatttgaatt tgcttgcggc atcccgggaa gtgtcggtgg agcggtttat
    15721 atgaatgcag gtgcgtatgg cggagagatc aaagatgttt ttgctgaagt ggatcttttg
    15781 cttgaagatg gaacattgaa aactttgaca aaagacgaaa tggtattttc ttatcgtcac
    15841 agtaaagtcc aagaattacg tgctatcgta ttggaagctc gattcagttt gcaaacaggg
    15901 gattatgaag caatcaaagc aagaatggat gagttgaccg aattgcgtca atcaaaacag
    15961 ccattagaat acccttcatg cggaagtgtc tttaagcgcc cagtaggaca ctatacaggt
    16021 cagctgatcc aacaagcagg attgcaaggc ctgaaatggg gcggtgcaca agtctctgaa
    16081 aaacatgctg gattcatcgt caatatcgat catgcgacag cgacagatta tattgaattg
    16141 attgcgcaca tccaagaagt aatcaaagaa aaatttgatg tgtctttaga aacagaagtg
    16201 cggatcattg gagaaaaaca agcaatatca gaaacaagca gaagtttagc caattaataa
    16261 gtaatggaac ttaaaataag taaaagagca ggacggtggc cgtcctgctc ttttttgtgg
    16321 ttgtttacgc aaatggcgca aatcttgtac attttaacac aataaaacaa aaaatataga
    16381 atatttcctc ataaattttt ccattttttg caatattctc aaaaaaaaga taaaaaatga
    16441 gaataactga tgaaaaaaca agcataatcc atcataaaat cagtataggt gaaaagatga
    16501 actttcatct gcatatcaag taattggtat gaggcatttt ttgaggtgta ggatatgaag
    16561 ttggattacc ttatagaaga ggaagataga gaaaaactgc tgctagctag atatttagaa
    16621 tttgagaaaa agaattatgt agtgctgcct aagttatatg aaacattagg tttgtctaaa
    16681 tttaaaacaa gaaattatat catagaatta aataaagaat ttactgaatt caaaacaaag
    16741 ccagagatcc aaatttcaga agaagcagag atccatgtcg aacgtctgaa tttgacggat
    16801 atcaaaaatt taagaagaat ctactttcaa cgatcaaaaa ttgctcgact gttaacggag
    16861 atcgtggatt caaaagtaac gatcgaaaaa tttgcagaga atcatttttt aagcatatca
    16921 agagcctatg taaaaagaag agaattagtc tcttttcttg aacagcatca aataaagtta
    16981 aagaaaaaca tattagttgg ggaagaaata aacatacgga acgtattatt tggggtatat
    17041 ttcggggtct ttaatggttt ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag
    17101 agtatcgtgc aatattttga atatctgttc aatttaaaac tgtctgcaac aaaacgaatc
    17161 aaattcaatt tgctaaccac tattagtttg ttgagggcct atgggaatca accaatcgga
    17221 aaacgtttct tttcagatga atactttaag agctcagaag gaatgaagat catcaaggat
    17281 ttatcacata tttcttttct tcatatccct aaagagcaga tcattgatga gatttcttat
    17341 atcatgttgt ttttgatcac ggaagaagaa tttgaattag atggcgcatt tggacttgcg
    17401 gaagctcatt ttcatgatat ccacatgatt agtcaaaaaa tgtcagtgga attgattgat
    17461 caattaccat tttcgattga aatctctgaa gaagaaagac tatatatacg aaaagaactt
    17521 cgtatgggta tgatgcatgt caatcgaaaa aattggatat tcgactttga gatgactagc
    17581 tttacgtcta aaaagcagct acagtttttc ttcgagacgt atccaatatt cagtacagca
    17641 atccaaaagg tcatacaaga gaataaacgc tatttcttgg gaaaagatga acgtttcatg
    17701 attcgcttat tttatgatta tctcttttta ttggtcggta tttgtccggt atcatatttt
    17761 gaagcaccga ttcatgtctg tattgatttt tcgcaaggat ctgcttatac cgattacatt
    17821 atttctcaag tgaaaggatt taaaaattac aatattgtga ttgagagtcg aatcacttct
    17881 cgtacgaata tttatttatc tgattgttta cttgaaaaat ttaataaaga acagataatt
    17941 tggaaaaatc caccgactcc agatgactgg gaatattttg gaaataccat catcagagaa
    18001 aaaaatagac atttaactga aaaaatcagc tagtatttat ggatggcagc tttgtctcac
    18061 atatttatta gctgatttat gtttttttca aagaaaaggt aactagatat aactagatat
    18121 aactagatat attacttaac aaaattaaaa agtacataag ttattattcc gattttcttg
    18181 aaacaataaa aaatcaaaaa aaatactctc ctaatagtcc atcttctttt gattttattt
    18241 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
    18301 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
    18361 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
    18421 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
    18481 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
    18541 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
    18601 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
    18661 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
    18721 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
    18781 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
    18841 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
    18901 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
    18961 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
    19021 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
    19081 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
    19141 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
    19201 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
    19261 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
    19321 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
    19381 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
    19441 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
    19501 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
    19561 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
    19621 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
    19681 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
    19741 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
    19801 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
    19861 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacgaga agaaagcttg
    19921 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
    19981 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
    20041 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
    20101 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
    20161 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
    20221 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
    20281 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
    20341 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
    20401 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
    20461 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
    20521 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
    20581 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
    20641 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
    20701 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
    20761 gtatccggtt tttcattggt tagcagtgaa accagattac caaggaaaag ggctcgcaaa
    20821 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
    20881 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
    20941 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
    21001 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
    21061 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg
    21121 catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg ccccttctct
    21181 tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg atgaacgtaa
    21241 atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca actgctccaa
    21301 taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag taaccatttt
    21361 ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt gtccgagtga
    21421 tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta ttgatttgct
    21481 ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa aattccacaa
    21541 actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct agtaataaag
    21601 gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa aagccgacac
    21661 ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa aaatagccat
    21721 aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac aaaatgattt
    21781 ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg atttcaaata
    21841 aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag acctgatcca
    21901 ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg attctacctg
    21961 cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa ctccttcatg
    22021 tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa
    22081 cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag ggggatacaa
    22141 atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat gagcgcatgc
    22201 ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc tgccgaagta
    22261 tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga atcttatgat
    22321 gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc aaaagatgct
    22381 gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac atctaaatca
    22441 tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat caaagactcg
    22501 aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga taaagaaatg
    22561 cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca aaacggtatc
    22621 aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca tcgtccgtct
    22681 gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt ccatgagcgg
    22741 gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg ggaaacggtc
    22801 aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc
    22861 gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata tcgtcccgat
    22921 ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat caccatgatt
    22981 gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc aactgtgcag
    23041 caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat cttggtcgat
    23101 caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc tgcagcaatt
    23161 acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc
    23221 acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa
    23281 ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga tggatggaat
    23341 acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat ggaacatgac
    23401 ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta cacgatgggc
    23461 ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc aattaggggg
    23521 ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat cggtggaaat
    23581 tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc tcgatttgct
    23641 tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc
    23701 taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa aaagctgccc
    23761 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggttgg
    23821 acttattacc gcagatgcaa gcttttgaac accgtttatt cggaaaaaga gcgtgggaca
    23881 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagaa
    23941 attccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
    24001 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttgatcag catgatttta
    24061 ttcagttatc ttcaaaatga tttttccgat gtttttgttt gcttccatat attcatgcgc
    24121 ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt ggccgaatct tgccattttc
    24181 tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc tctgctttat actgatcact
    24241 tctaggtgtc aataatgtac caagcagttg gacacgtttc ccaatcaata caccaaggtc
    24301 tatctcaggc acgatggtcc cacctagcat accaatcaag acccaacgtc catcaacggc
    24361 tatagaagct aaattctttt cccagtaaga agcgccgatg aaatccaaaa taacatcgac
    24421 tccccgacct tcagtgattt ttcgtacctc ttcttgaaag tcttgttttt tatagttgat
    24481 caattcatct gcaccaagtt ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac
    24541 gatgattttc gcgtgagaaa gctgtctagc taattggatc gctgctgttc ccacaccact
    24601 tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt
    24661 ttgataagca gtcaaaaaaa cttctgagat tccagcagca gaaacatagt ctagcgtatc
    24721 agggagaagg attgcacgat cggcaggcat cacagcatat tcggcataac cacctagatt
    24781 gactaatccg tatacttttg tcccaggagt aaaagtggag atatcacttt tattctcaac
    24841 tacctctcca gctacttcga ctcccaaaat agggtaggga ggtttcaaag agactgattc
    24901 tcttttcata atatctgttc gattgacagc tgctgtatgg actttgatca gtaattcgcc
    24961 ttgttttggt ttaggaacag gcacttcaat ttgttctaat acatctacag aaccaggatg
    25021 cttgattgaa atggcacgca tggatctatt cctcctttgc ccattcttgg aatgctgtat
    25081 tcaactcttt gctaccttca gaaagtacta gagtcgtacg agaaatatcc gtttcttcca
    25141 ctagagcggc aagtacagca gcaactgtag agcgagtgat cgtttttgat gccttctcat
    25201 taggcttagt taattgaatg gaagtaactt cttcatcgtt agtcaatgtg actggttgaa
    25261 gaatgacaaa atctaagtta gttcgtttca gccattcatc tgcataatgt ttagtaatat
    25321 aataatctgt catggactca ggccattttg ctggttcatc tgcataaaca gcactgacca
    25381 tcagatagcg agagacatgc gcattttcag ctgcgatcac tgttttgatt gcgccatcca
    25441 agtctacttg cagaagattc tttccttgag agccggcagc aaaaatcaat acatcgatgc
    25501 ctttgaatgc ttcggtcatt ttttccacgg accaagttaa atcaaaagag acatacgaaa
    25561 cattctcttt ttttactaat gtttgggaag caacatctct cacccctgca aaaatttcat
    25621 gggaacttgg agctaaatct tttatcaggt gtcggccaat ttgtccgttt gcacctacaa
    25681 caaaaatttt catttggatc atcctttcga cgtcaaatct ttccattcaa tagtaacacc
    25741 aaaagagaaa aaaatcagag atcggctacg atgactatca gttagtaaga gaagggtgct
    25801 tgaagtatct taacaagaca ttgcatactg ctgcagtcgc aagacctgcg acgattcctt
    25861 caggtatacc acttgtaaaa ataaccgcta gaatagctga ataaacagca tttaaatttg
    25921 ttccgatgac tttggcataa tcttgctgga ataaaaagta gatcatattc atcactaaaa
    25981 tcgtattggt caatgaaccg gaaagtccag ctaagaataa agaaacggaa tctgcttttt
    26041 gtttcattag tttgcgcaga cctttataaa cgaaataagg aaccacccca atcaagatgc
    26101 gaggaataaa cgcgatcagc aaggctttcc agcttccatg ttctgttccg atgactggga
    26161 taaacggaga aaagacaaaa gacaaaggtg tttggataac tgtgctacgg atcatgctga
    26221 tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa
    26281 tgattacagg aatatgcatg gttgttgcat tgattggacc gatagggata aaacctaaag
    26341 gcgtaacagc taataaaatc aaaatagcta aaaacatcgc agttaatgta aagttttttg
    26401 tatttttcat aagtactcct tttttctaag catttctttc attaagagtc agcatgacct
    26461 gttcgatgat cgtctgaata tcagctaagg caccttttcc aaaatctcca caagctaaaa
    26521 gagattctct tggttcaatt tcctgatagc cgacagatct caatgtttcc agattacgct
    26581 gagtgatcgg gttttgatac atattcgtgt tcatggcagg ggcaatcaat tttggtgttc
    26641 ccactggaag cgccattgcg actgttgaaa gaagatcgtc tgcaatgccg tttgctagct
    26701 tgccgataat gtttgctgta gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt
    26761 cgatatgatt gattacctgc ggatttgatt cttgcatgac gtctgtatgg actgcgcgct
    26821 tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt gctgctttta gacataatca
    26881 catctacctg ggcacctaat ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag
    26941 aaatacttcc agtaacgcct agtaagatct ttttcatttt gattcctcca gtctaatttt
    27001 ctcagtaatc aatgcagcaa tctcagattt actttgagct tcttccactg ttccatcttt
    27061 agataagaga tagccgtggt gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat
    27121 aaaatcagtt ttgttattta tcagactatt ttttgcgact gccagaagag attcctttgg
    27181 tacatctact aatagtttga aaccaatgat ttttgctttt ggctgccatt cacgtagata
    27241 agaaatgatt tttggtgttt tcttcaaaac gagcacaaga tgctcggtgt tagaagagat
    27301 ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac caattgtcca gttcttcttg
    27361 cgttgaagaa gtgggtaggt tttttgccag ctgttcttct gaaaaactaa aagcaggagt
    27421 aaaatcactg actgccatac tgtggataat cgcatcgtag tgttcttttt tcgttagttc
    27481 ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta taaatcgtta tattggagtg
    27541 tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg attgtggtat ccggattggc
    27601 agctaaataa tcagctaatg cttgccctaa tcgtcctgtc gaatgatttg taatagcacg
    27661 tacttgatcg attttttcag tcgttcctcc agctgtgatt agaatcttca tttatggtca
    27721 cctacattta ctagtgttct tccttggtgt gttccagcaa gtagtttaga tgctgtttca
    27781 ggcaattgat ccagagtaat ctcttggacg accagttcat ctgcaatctg caaatccgcc
    27841 atctgttgcc ataaagaaag acgttgatct attggtacat tgacagaatc aattccaatc
    27901 aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt ttagtttgat tcccccagca
    27961 ttgccacata aagctactgc accgttataa gagatcaatg gaagaagact gcttagctgc
    28021 tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc
    28081 tctggcagaa attcatcagg tcgtacgact tgcaaagcac ctagactttt tagccatgtg
    28141 acagactctt cttttctgga aaaggcaatg attgactgga atcctagttt atgtaacagg
    28201 gctatcgctg tactgcctac accgccagaa gcaccagtaa cgacaacttt tttatcggag
    28261 gtcatacctt gacggattaa agcgttcaca cacaacatag cagtaaaccc tgctgtacca
    28321 aggatcatcg cttgacgcag cgtcatattt tttggtagag gaactaacca gtctcctggt
    28381 actttttgat attggctata accgcctgga tgggtgacgc caagcccaaa accagtagca
    28441 agtacaagat cttcttttga aaaacgtgga ttgttacttt ggacgacagt tccggataaa
    28501 tcaatccctg gagtcatcgg atatgttcgt atcacctgac cagactcgct cattgccaat
    28561 gcatctttat aattgacatc agaataggca atttttacca agacttcatt ttcagccaac
    28621 tcagtaaatg gcagatcact gattgctgta tggaaaacag gttcctttat tacctgaaaa
    28681 gatcgaaaag cgttcattcg tgagacactc cttttaaaat tttcttttat cataccataa
    28741 agcctactct tttattcttg ttcttccgta aatttagaca aaaattaggt gaaaaataaa
    28801 gaatcaaaca actagaattt attccaaaag ataaagaaat attcagcaaa aaaaatttta
    28861 caaagggttg cctttttcaa taaatttcgt atactaatac gagtggtagt aaacaggaag
    28921 tttactatta gaaaacttcg attcggagga aattatggaa attggtgaga agttaagaaa
    28981 tttgcgcatc caaaagaatt tgacacaaga agaactaggc gaacgcacag atttgtcaaa
    29041 agggtatatt tcccagttgg aacgagattt aagttcacca tcaatggaaa cattcttttc
    29101 tattcttgaa gtcttaggtg tgactccaga agaatttttt catcaagaag cagcgaatct
    29161 gcaagtggtt tattcaaaag aagatcatac ggtttatttc gatgaagaaa acgggtatga
    29221 attggaatgg ctcgtggctg attcgaatga aaaagaaatg gagcctgtct tgctgacttt
    29281 tgaaggtgca ggagaatata agacgtttga accttcatta tctgaaacgt ttatttttgt
    29341 attagaaggg gaattagagt taagcttagg agagacggtc tatgtggcaa aagcaggaca
    29401 gtcgatctat tatcaagcga caaaacaaca tcaattaagg aatcatctca aaaaacgaac
    29461 aaaagtattg attgttgcaa cgcaatcata tttgtaatat agggggtaaa aacgtgggga
    29521 aaccaatcat ttcatttgat caagtagtga aacgctatga tgatgaaaca gtattaaaaa
    29581 aagtaagttt tgagatcgaa caaggaaaat tttatacact gcttggtcct tccggctgtg
    29641 gtaaaacaac gatcctacgt attatagctg gattcaccga agctacagaa ggggacatct
    29701 attttgaagg gaaacggttg aatgatttgc cagctaataa gcgacaagta aatacagtct
    29761 tccaagatta tgcacttttt cctcatatga atgtttttga taacgttgct tttggattga
    29821 agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg
    29881 tccagcttcc tggatacgaa aaacgggaga ttagtgaaat gtctggtggt caaagacagc
    29941 gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt attgctatta gatgaaccgt
    30001 tatcggcgct tgatttgaaa ctacgtacag atatgcagta tgaactgcgg gatctgcaac
    30061 aacgattagg aatcacgttt atttttgtta ctcatgacca agaagaagcg ttggcaatga
    30121 gtgatgagat ttttgtcatg aataaaggga aaatcgtcca aagtggtaca cccgtagata
    30181 tttatgatga gccaatcaat catttcgttg ctgattttgt tggggagagc aatatcgtgg
    30241 acggtgtaat gatcgaagat aatctagttg agtttgttgg taagcaattt gaatgtgtcg
    30301 atggcgggat gcgtctaaat gaaccagttg aagttgtttt acgcccagag gatttgacga
    30361 tcacgacacc agataaagga aaactagtcg taacggtcga tactcagctg tttcgtggtg
    30421 tccattatga aatcatctgt tatgatgaac agcaaaatga atggatggtc cattcaacga
    30481 aaaaagctaa agaaggaagc aaagtaggat tggcctttga gccagaagat atccatgtca
    30541 tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct tgacagctac gaagaatagg
    30601 ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc ccttatattt tgtggctcgg
    30661 tttattcgtg atcgcgcctg tattaatgat catttatcaa tctttttttg atatgaatgg
    30721 gcaattcacg ctgaataatt atctatctta ttttacgtcg gggacatcgc tttctatgac
    30781 gatcaattct gtatggtatg catttttgat cacgtttttc acgctgatca tcagttaccc
    30841 gactgcttat tttttaacaa aactgaagca caagcaatta tggctgatgc tgattatttt
    30901 gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt attgggattt tcagtgtgca
    30961 cggcagcgtg aatcatttct ttgattttat cgggatcggg ccgaaacaga tattattcac
    31021 agacttcagt tttctgttcg tggcaacgta tatcgagatt ccatttatga ttatgccaat
    31081 tttcaatgca ttggaagaga tgaatccttc tttgatcagt gctagtagag atttaggcgc
    31141 gaatagcttt gagaccttcc gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag
    31201 tggtgtccaa gcagtattta ttccttctct ttctttattc atgctgactc gtctgattgg
    31261 cggaaatcgg gtgatcactt taggaacggc tatcgaacag catttcttgg ttacacaaaa
    31321 ctgggggatg ggttctacaa tcggtgtgat tttaatcatt gctatgttca tcgtgatgct
    31381 cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg aaaaaattta aatggtcgta
    31441 tctttattta gggatcgtct ttttattctt atatgcaccg atcttctatc tgatttttta
    31501 ttccttcaat gaaggaggaa cgatgaacag ctttacagga tttacttggg aaaattatga
    31561 agctgttttt gaagatacac gtttgatcat tatcgtattg aatacgttca tgctggcttt
    31621 cttatctgca ttgatcgcca cgatcatcgg gacatttggg gctatgggaa tctattacac
    31681 gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat aacatcttat tagtctctcc
    31741 tgatgttatt atcggtgcta gtttcttgat tttctttaca ctactaggat tcggattagg
    31801 ttttcagagt gtattgcttt cccatattgc tttcagcatt ccaatcgtcg tcttgatggt
    31861 cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat gcggctagag accttggtgc
    31921 taataatttc caagtagtca aaaatatcat tttgccttat ctttctccag gaatcatagc
    31981 tggatatttt atggcattta cctattctct tgatgatttt gcggttacat ttttcgtcac
    32041 aggaaatggc tttacgacgc tctcagtaga gatctattct cgtgcaagac aaggaatcag
    32101 tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc tccatgatat tggtcattgg
    32161 atactacttt attagtaaag aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg
    32221 cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc tgtttttagg tattctcatc
    32281 attattgcgg tgctgctgct tggtgttcgt caacttgaaa aaacaagtgg catgagtgga
    32341 gcaaaggtac ttaccatcta taactgggga gattatatcg atcctagttt gatcacgaaa
    32401 tttgaaaaag aatatggata caaagtgaat tatgaaacat ttgattctaa tgaagcgatg
    32461 ttcacaaaaa tccagcaagg tgggacaaac tacgacatcg ctataccaag tgaatatatg
    32521 atccaaaaaa tgatcaaaga aaaactagta cttccattag atcattcgaa gatcaaagga
    32581 ttaaataata ttgatccacg atttttagac ttggattttg atccagataa tcagtattcg
    32641 attccatatt tttggggaac tttagggatc gtatataacg ataaattctt tgatgcaaat
    32701 caaattaagc attgggatga tttgtggaag ccggaattaa aagacagttt gatgctgatt
    32761 gacggtgcac gagaagttat ggggctttct ttgaatagtt taggctattc tttgaatagt
    32821 aagaatatgg cacagttgac agaagcagca aataaattga atcatttaac accaaatgtc
    32881 aaagcaatcg tggcagatga gatcaaaatg tatatgatca atgaagaagc ctccgtggca
    32941 gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa atgaacattt acattatgtg
    33001 atcccaccag aaggatccaa tctttggttt gataatatcg ttatgccaaa aaccgtgaaa
    33061 aataaagaag gtgcctatga tttcatcaac tttatgctag aaccggaaaa tgcggcacaa
    33121 aatgcagagt atattggtta ttctacacca aatgaagaag caaaagcgtt attgccaaaa
    33181 gacatttcgt cggacgaaca attctatcca tctgatgaca cgatctccca tcttgaagtc
    33241 tacaaagatc taggatcgaa atacctaggc atctataatg acctattctt agaattcaag
    33301 atgtatagga gataatggca aaactcagta agaatgggga cttatcaacg ttctaaagag
    33361 ctaaaaatcg tcctttttct atctgagttt tccaatcttc taccaaaagc ggatgaaacg
    33421 ccgctttttt tgtgttattt tattttttct accacgtttt tctaccagag aaaagtggac
    33481 aaaaccattg taaaataggg cccgagacgg gctagtaaat ctaagattga aaaagttaca
    33541 gaaggataaa gtaattggaa actatgcaaa tatacttaac ccttctgtaa tctattaata
    33601 aaactaatat atgaatccag ttcaatattt gtagctatga aaaaacataa aaatcatatt
    33661 agataaaaac taaattggta gataaattat tttgtcagat atctgaggca aatattcgac
    33721 tatttgtgtt tcaaagtaaa aattggaata caaatataga atagacatat tgcagccgat
    33781 agtttattat gcttttagac acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga
    33841 tcgagttttt gatgagctga tacgactaac agggaatatg tcttctgtca ctgccgagaa
    33901 aatttcgaag aatctaggta tctctcgtca gaatgccagc cattatttga cgagattagt
    33961 ggaagataaa aaggtcgaga aattaccggg aaagcctgtt ttgtggaagc ctttagatga
    34021 atatgcggtt gtagataata cggagcaaat aaacgaagcc tttcattctg tagttggtca
    34081 tgacggaagt ctgagagagg tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa
    34141 cggactcagt gtattaataa atggagccac gggtgttgga aaaagttttt tagccacaaa
    34201 aatttttgaa tacgctatcc atgagcaaat tattgaaaaa gatgcaccat ttgctatctt
    34261 aaactgtgcc gattatgcgg acaatcccga actgctttcg gcaacactgt ttggatacaa
    34321 gaagggggcc tttacggggg ctgaaaagga taccgatggt ttattggcaa cggccaacaa
    34381 cggctatctt tttctcgatg aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt
    34441 tctatttatt gatactggaa actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc
    34501 taaggttcgt tttattttcg ccacgacgga aaaaggtgaa aattatttat tagacacctt
    34561 tgatcggcgt attcaaatta gtgttatgtt gccaactttt gaagatcgcc ccattcgaga
    34621 acgattagag ctgattcaat tattttttca aaatgaagcg gatgttttac aaaaagatat
    34681 tattgtttca agagaagcct tgtctttaat gttatctcat ccctttagtg ggaatattgg
    34741 taagctgaag aatatcatta aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga
    34801 accattaacg attgggaaaa atgagatctt gattcagtta aatatgctgg aatccgaagt
    34861 agaaagcttg ccgattgcta gtttattgat aaagaaaaat caaaagataa aaacggatcg
    34921 aatcaaaccg tttgattatg attcgatttt ggaaaaaatt actcagctga taaatgaagg
    34981 taatttcaat gaaaattttt cttcgataaa gaatgaagta cagcatttgg tgcatcacat
    35041 tgataaggat tcttttttat caatgagtag aaaattgatg attcagatgt ttagaaaggt
    35101 ctgggtatcc gttttaggaa aaacgtacgg attgacgacg agtggacctg ttgcagaaat
    35161 tattagcaga tcctatgtca tttccccaag ctataagaca gacttaaccg ggataaatcg
    35221 tacattagct gcaaatctac ctcggacgac ttacttatgt aatcagttct tggagcattt
    35281 accaccgctc tcagaaaagg acgagaactt tctaagactg cttattgtcg tgactttatc
    35341 tgattatgta gatgaatcca tcgagctgaa gggcctgttg cttgctcacg gagatagtac
    35401 ggctagcagc attcaagcag tagtgaatca attatgcggc aattatgttt ttgaagcgtt
    35461 ggatatgcca gtagagagta atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa
    35521 acggcaagtg aatacagata gtctagtgct gatcgtagat atgggctctt taaatcaaat
    35581 ttacacacag atcaagaacg atttaaaagg ggagttactt cttctgaata atttgacgac
    35641 ttccattgca cttgatattg gcttgaagat gacgatgaat gcgccattta aacagattgc
    35701 ggaggatgca gcagacaagt attcaatcaa tgttcaatac tttgaaggct tttctcaaag
    35761 tagcaacatt atcatttcct gtatgtcggg tttggggatt tcggacaaat tgaaagatgt
    35821 ttttcgtcat catttatcgg atgaatccat cgaagttttc acgaaggact acagagaact
    35881 acgtgaatta atcgaccaaa atgatgaaac ttattttgac aagacaaaat tggtcatcac
    35941 gacttcggat cttccacgtt ccttttcaat tcctaatatt aatgtttatg acatcttaga
    36001 cggtgatgga gcagccaatt tggagcatat tttgagtcct gaaatgaagc cggcagaatt
    36061 taaccgcttg attcaggagc tagtaaaatt cttttcgttg gaaggaattg cggaccgttt
    36121 gaactttctg aacccaacga tcgtgatgag tgaagtggaa acagtcattt ctaaatatga
    36181 aaattactat catttcactt taaatggccg tgtaaaacta aatctctata tgcatacggc
    36241 attgatgatg gaacgtttat tcttggctcg gacagaaaaa tctgaagaac cgaattatga
    36301 tttgaaacca gatgaagatg aattttatta tgtttctaga agtatttttc aatcaatgga
    36361 gatgaaatat aattttaaag tcaatgctta tgagctttcg ctgctctatg aattattagg
    36421 accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga
    36481 cacttttttt aatgtgaatt aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa
    36541 ttgcttatat aaaagtgaac agaaagaaag gaattaacca tatgaagaaa attctaatag
    36601 cgactcacgg tcacttagct tcaggctttc ttagcagtat tcaattactg acggggaagg
    36661 ttcaagagat tacggtgatt aatgcttatg tggatgagtg tgattttgaa caggagttga
    36721 atgtatttgt aaaccaggtg aacgagcagg atcagatttt tgtttttacc gatttatttg
    36781 gcggaagtgt caatcagaag attaccaaaa cctttttgga gaaagagatt gctgtaacta
    36841 ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt attggcaacc aaggaattaa
    36901 caaaggagga tattcaggaa ctggtaaata aatgccaaca ggaattaaag atgaccattc
    36961 ttgatgaggt acatgtagta aatgaagagg aagatttttt ttaggaggaa gaaaaatgat
    37021 taaagcagtg cgaattgatg aacgattaat tcatggacaa gtagcgatga cttggacaaa
    37081 gagtctaaat ttaaccgggt tagtagtagc aagcgatgag gcggctacca acgatattca
    37141 aaagatgaca ttgaagatgg ctgcaccatc gaatgtcaaa gtcattatta aaacagttga
    37201 tgacgcgatt catttgttaa aagatccgcg ggcgaaggac atgcgcttga tggttttagt
    37261 ccctaatgtc aaagatgcgg taaaagttgc gaaagctttc ccaacggaaa tcgaattaat
    37321 gaacgtgggg aatgctggga aaatgacacc gccatcggaa gataagaaaa ccttatcaaa
    37381 ggaagtgatg ttaacgacgt cagaaattca agacttaaaa gaattagttg aactttatcc
    37441 agataccgtt tttcagccta caccggcgat ggaaaaacgt tcagctatct ctattttgag
    37501 tgatttttag aaaaaggaag gagaaaaatt atgtatgatg catttgtagt cgctttggcc
    37561 gtttttattg gaattgctgg acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt
    37621 gttgttgctc cgttagcagg agcgttgatg ggggatttgc agacggggtt gattgtcggg
    37681 gcgtcactag aagccatctt tatgggtgtc gtcaatatcg gaatctctag tacggcggag
    37741 ccttctttgg ctgctgggct ggctacagcg ttcgcaatca aaatgggagg aaacgtggat
    37801 gcgattattc ccattgtctt tccactagca gtattaggat tacagttaat gaatatgatt
    37861 ttttcttttg tatgtggacc gatggctgct aaatttttca aatttgcaaa atctggtgat
    37921 caaaaaggaa tcatcaatct gcacttcttt atgtggtttg tccactatgc gctttatgct
    37981 cttattccat tctttgccgt tttatttgga gcagatgttg tcaaaagcgt cttggataat
    38041 attccagagg tcatcatgaa cggattaaca gtcgctggaa atctgctgcc ggcagttggg
    38101 atggcgatgc tgttacggat gctttggagt aaaaatattg ctgtgtggtt tttctttggg
    38161 gccgttgtta tggcgtatct cgagttacca ttgattgcaa ttgccgcagt tggaacgatt
    38221 attgcaattg ttatagccca aagagatcta caactaaaaa atatatctgt tcatgcagtt
    38281 caaagcgatg ctgctgtaaa tgcaacgctt gaccaagaag aggaggattt ctttcaatga
    38341 aattagctga tggaatttct aaagatgaga aaaaattggt tcggaagatg ttttggcgct
    38401 cgatgactat gtatgcttcg gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt
    38461 ctatgatgcc cttcattaat catttctaca aaagtgaaga agatcgagct gcggcgctgg
    38521 aacgtcatac acagtatttt tcgacgacca ttccttgcgc gtcctttgtc atggggattg
    38581 cggcatcgat ggagaaggaa aattctgaaa aagctggttt tgacgtggaa agtatcaacg
    38641 cgatcaaaac gagtttgatg ggaccattgg ctggaattgg cgattcattg ttctggggag
    38701 tttggcgtgt cgttaccgcg ggactagcga tcaacttagc caatgccgga aatattttgg
    38761 caccaattat ctttttagtt atgttcaata tacctaatta cttgatgcga tactatggcg
    38821 gcgttttagg ctattcatta ggctctaaat atattgaaaa gctgtatgcg aacgggttaa
    38881 ttgaaattct gacaaaagct gctagtatct tgggtctgat catggtaggt gcaatgacga
    38941 tcagtatggt caaattcaat accgttttga gcttttcaat gaagggcgaa gaactaatga
    39001 atgtccaaga aatcctcgat agtattttta aaggaattgt accgttattg gtaacattga
    39061 gctgtttcaa attactagat aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg
    39121 tcttgggaat tttattgtca ttacttggaa tcgcatagta ggaagctgca gctactaggc
    39181 tgcagttttt ttagcaagga gggaaaagat gactattaat gaagtaattt attcacccaa
    39241 acacccattt tttaatgctg agttaaacga cggaaatgtg actacttcaa acgaatacag
    39301 ctactatcgg aatattcctg atgtaaaggg attgaccttc gatgtaagta atcaaaagac
    39361 attagcaaac cttgatgttt gcgggacgat tcaaacgttg accttttatc gagaaaattt
    39421 actaactgag gaaaaaccag gtgtttgggt caataaacag ctctctcaaa ccaattcgtt
    39481 atccttaaag gttgaagtaa acgggattgt ctaccatttg tcagaatcgg atcatcgtat
    39541 tgaagtcgat gtgattcaag actgtttgcc tagatatatt catcattatt cgacgtttaa
    39601 ggtcattgta gtatcctttg ctcccatact ggctaataag cgtttaagta tgcttgttca
    39661 acaggtttac attgtgaatg aaacacacga aagccttcaa gtttcagtga aagatgtgcc
    39721 ttcgtatcaa gagaagtatt cggatcagca aaatgtgttg atttctcaaa aaggaaataa
    39781 ggacacgatc gcacaacagg aagtggcaag cttcgccatt gcttttgtgg acccaaatgc
    39841 ttttgaagaa gtaatgactt ttcaggaatc tgatatcgaa aaatggctga aggaaacatt
    39901 gaactatttt gctcagctat ttgggcagct gtcaatgcca aacgatagag ttgtccatct
    39961 ctatcgtcgt gcactttatc aaagcttttc tagtttcggt atgaatcgac agggacaggt
    40021 cgtcggcagc aattggggca gctatcctgc tactaatcga atttggaata aggatatgta
    40081 ttacgcatca ttgccattca ccatgtttga accggagtta tgtcaaaaaa cggttctatg
    40141 gtttgatcaa tatggcgtta agtttcctgg ttcaaaattt acaggaggta tcaatcattc
    40201 tctgagtaat tcgttatcca gtgcgttact ggctagtctt tattttgaac atactgggga
    40261 tacaaaattc tttgagcggt gtccgcaggt tctactgaat gcttcaagaa ttattgagga
    40321 catattagct caacgtcggt caggggaacc aatgcttttt ccatctgttt ggttatcaga
    40381 cgcctttgca cttggaaagt atcataccgg cagcaatctt tgtctttgga aggcctgtat
    40441 tggacttgca gaaatttttg aagccctaga acaactgagt cttagcaagc gatacaaaaa
    40501 cattgcctgt aaactaaagg aatcaatttg taaacaaatg actatcgatg gtccttttgg
    40561 tgagcaattt ttggagggaa tcggtgatga ggagaaaaca acctattcag tgaagcacta
    40621 tcaaaaaccg attcttgagc aagggttaat ctttttaagt gatgtgattg tggatggaaa
    40681 aattaattta ttgatgcatg atggagaaga atcagacact acgttgatgc ctttttatca
    40741 atttttagat aataaagatc atttatatca gaacaccatg gccttttctg ctagttcttt
    40801 caatccaacc tatagcgagg aaatcaaagg catcacttgg ggattagaat ccggcgccac
    40861 ttttcctggc tttatcactg ttttaatgag cgagctgcac aaccctcaag ctttcgtaac
    40921 ccgattagaa gaactaattt gtttggcaga tttagacggt tcgtggtggt ggtggccgta
    40981 tcgcttagag gctcaacaag gagaagtcgt gcgagacttt ggctgcggca aatgcggctg
    41041 ggcatcgggt ctttttgtca gcctaatgat cagtcaatat ttcggattga aattcaaaaa
    41101 aggagtattg caaattgatc ctgttgataa tttaacgttt tcttggaaga atttgaagtt
    41161 cggtcaagcc ttcatttcga ttgaatgtac gcaatccgag ttgagtattt caaatttgtc
    41221 agaaaagcca ttgaaaataa gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac
    41281 gattcacatt caaaggagaa aaagatgagt atactaaaaa atattaccca atttaaaaaa
    41341 atccagagtc gagcaatcac tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg
    41401 ttgccaagcg agctaggacc aagccgcaaa ggtcgtggcg caattccgct gacttctggt
    41461 gaagaaactg tgattgcgga tattactgga acgggaataa ttcgtcatat gtggatgacg
    41521 attcgtgatc gaacagaatt tggcagtttt gttttacgtg atttaatctt aagaatttat
    41581 tgggatgggt cggatagccc tgctgttgaa agtcctttag gggatttctt ttgtaatggg
    41641 tttggagagc gttgcgaggt gaactcgttg ccgatagtgg ttaatcctac agggggcttt
    41701 aattcctatt tcgaaatgcc tttccaaaca tccgctaaaa ttacgatcga gaatttacat
    41761 ccgaaggata tgtcctcatt tttttatacg ataaattacg ctttggtgga cgaattggaa
    41821 gaaaatagcc tatattttca cgcaaattgg catcgggaac gcattaccaa attacaggag
    41881 gattataagt taattgatcg attaaagggt caaggctatt acgtgggtac ttaccttgcg
    41941 cttacctgcc tagaacgtta ttggtggggc gaaggcgagt ttaaattcta cattgatggg
    42001 gatagtgagt atccgaccgt aacctcaaca ggttcagaag attattttgg cggggcgtgg
    42061 gcctttcatg agtacgatga gttcggacgt ccacatgcga aaaactattc aacagccttt
    42121 ttagggtatc cttaccaatc aaatagagat gccacaagag atcgttttga aacgggaaaa
    42181 ttgaatgccg ttcatgcctt tggtgacgac gcaatgccac gacatggtct ttatcgttgg
    42241 catcttcaag accccatctg ttttaccgaa gatttatcgg tcactttaca acaaatcggc
    42301 aacgatgatg ttcgcttatt tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat
    42361 tcaacagaag cttcaggaaa tactaaacgt catttcactg atcgagaacg cttgccgcgt
    42421 taatgaagtg tttttcacat attcgaaggg aggaacctat gatgatatat ggggcaattg
    42481 aagctggagg gactaaattt gtctgtgcag tcggtactga ggaattaacg attttggaaa
    42541 gagtcagttt tcccacaaca actccagttg aaacaatgaa gcaggtcatt gattttttta
    42601 aaccttttaa ggaacaattg gcaggcatcg gggtcggctg ctttggtcca attgatattc
    42661 aatgcaaatc aacgagtttc ggtcatatca catcaacacc aaaacttgct tggcaaaatt
    42721 tcaattttgt aggaacgcta aagcaatatt tcaatattcc gattgaatgg acaacagatg
    42781 tcaatgcggc ttgctatggt gaatatgttg ctggttctgg caaagggtta gcaggtgttg
    42841 catacttcac tgtgggaaca ggtatcggag gtggtgcctt gttcaatggg actttcgtgg
    42901 aagggttcag tcatccggaa atggggcata tgttgatcaa gcgacatcca aaggacaatt
    42961 tttcaggaaa ctgtcccttc caccaagatt gtttagaggg gatggcagca ggaccagcga
    43021 ttgaaaaacg gttgggtgta aaaggccaga atttattggc tgacgattcg ttttggcaga
    43081 ttgaagcctt ttaccttgcc caatgtgcat acaatacaac actgatgttt tcgccagata
    43141 gaattatttt tggcggtggt gtaatgaaac aagaacacat gaagaaaaaa gttcaggata
    43201 aatttgttga gcttataaat ggatatgttg aaataccacc aattgatagc tacattatta
    43261 cgcccgaatt aggtgataat gccggaataa tcggcggatt agcattggcc agaaaagcgg
    43321 taagaaataa gcaaccataa ttaatgaatg tttcattgat actcatgtca aaagtattta
    43381 accgagtaat caaatgatca aatgaagttg gataaagcga aaatgaattc aaataaaaag
    43441 gctctaagtc aaatcgtgtt gttttcatta aatcactgaa aggaaatgct tctgcgtttc
    43501 cttttttctt tttgttgtgc gctgtatacc ttgaggatat tcactggtgg cgtacactta
    43561 aatagtaata tattgtgtta ctttatgtct gtggtattag ttatagttgc ttaccaatgt
    43621 atgttcattt ttcgaaatgt tgacttcata atccttggct taatgtttga atttttttgg
    43681 tgatagattt tttcagccgt ataaaa
//
LOCUS       DAJSBC010000017        43572 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSBC010000017 DAJSBC010000000
VERSION     DAJSBC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.017"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(212..2593)
                     /locus_tag="ONT65_000958"
     CDS             complement(212..2593)
                     /locus_tag="ONT65_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2328608.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2583..3230)
                     /gene="recU"
                     /locus_tag="ONT65_000959"
     CDS             complement(2583..3230)
                     /gene="recU"
                     /locus_tag="ONT65_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR2328609.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3294..3833
                     /locus_tag="ONT65_000960"
     CDS             3294..3833
                     /locus_tag="ONT65_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR2328610.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3923..4342
                     /gene="gpsB"
                     /locus_tag="ONT65_000961"
     CDS             3923..4342
                     /gene="gpsB"
                     /locus_tag="ONT65_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR2328611.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4364..4731
                     /gene="rnpB"
                     /locus_tag="ONT65_000962"
     ncRNA           4364..4731
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ONT65_000962"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4940..6106
                     /locus_tag="ONT65_000963"
     CDS             4940..6106
                     /locus_tag="ONT65_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR2328612.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6152..7648
                     /locus_tag="ONT65_000964"
     CDS             6152..7648
                     /locus_tag="ONT65_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR2328613.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7729..8412
                     /locus_tag="ONT65_000965"
     CDS             7729..8412
                     /locus_tag="ONT65_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2328614.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9057..10247
                     /locus_tag="ONT65_000966"
     CDS             9057..10247
                     /locus_tag="ONT65_000966"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR2328615.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10349..11302
                     /locus_tag="ONT65_000967"
     CDS             10349..11302
                     /locus_tag="ONT65_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR2328616.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11371..12720
                     /locus_tag="ONT65_000968"
     CDS             11371..12720
                     /locus_tag="ONT65_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2328617.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12903..13226)
                     /locus_tag="ONT65_000969"
     CDS             complement(12903..13226)
                     /locus_tag="ONT65_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328618.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13355..14518
                     /locus_tag="ONT65_000970"
     CDS             13355..14518
                     /locus_tag="ONT65_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2328619.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14588..15937
                     /locus_tag="ONT65_000971"
     CDS             14588..15937
                     /locus_tag="ONT65_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2328620.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15982..16413)
                     /locus_tag="ONT65_000972"
     CDS             complement(15982..16413)
                     /locus_tag="ONT65_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2328621.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16801..19443
                     /gene="alaS"
                     /locus_tag="ONT65_000973"
     CDS             16801..19443
                     /gene="alaS"
                     /locus_tag="ONT65_000973"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR2328622.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19643..20239
                     /locus_tag="ONT65_000974"
     CDS             19643..20239
                     /locus_tag="ONT65_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2328623.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20361..21509
                     /locus_tag="ONT65_000975"
     CDS             20361..21509
                     /locus_tag="ONT65_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2328624.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21547..21960)
                     /locus_tag="ONT65_000976"
     CDS             complement(21547..21960)
                     /locus_tag="ONT65_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR2328625.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22039..22470
                     /locus_tag="ONT65_000977"
     CDS             22039..22470
                     /locus_tag="ONT65_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR2328626.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22569..22802)
                     /locus_tag="ONT65_000978"
     CDS             complement(22569..22802)
                     /locus_tag="ONT65_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR2328627.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22985..24655
                     /locus_tag="ONT65_000979"
     CDS             22985..24655
                     /locus_tag="ONT65_000979"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR2328628.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24802..25188
                     /locus_tag="ONT65_000980"
     CDS             24802..25188
                     /locus_tag="ONT65_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328629.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25175..25873
                     /locus_tag="ONT65_000981"
     CDS             25175..25873
                     /locus_tag="ONT65_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2328630.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25878..26342
                     /locus_tag="ONT65_000982"
     CDS             25878..26342
                     /locus_tag="ONT65_000982"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR2328631.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26339..27250
                     /locus_tag="ONT65_000983"
     CDS             26339..27250
                     /locus_tag="ONT65_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2328632.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27485..28024
                     /gene="pyrR"
                     /locus_tag="ONT65_000984"
     CDS             27485..28024
                     /gene="pyrR"
                     /locus_tag="ONT65_000984"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR2328633.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28038..29321
                     /locus_tag="ONT65_000985"
     CDS             28038..29321
                     /locus_tag="ONT65_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR2328634.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29334..30260
                     /locus_tag="ONT65_000986"
     CDS             29334..30260
                     /locus_tag="ONT65_000986"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR2328635.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30279..31559
                     /locus_tag="ONT65_000987"
     CDS             30279..31559
                     /locus_tag="ONT65_000987"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR2328636.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31564..32640
                     /gene="carA"
                     /locus_tag="ONT65_000988"
     CDS             31564..32640
                     /gene="carA"
                     /locus_tag="ONT65_000988"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR2328637.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32645..35830
                     /gene="carB"
                     /locus_tag="ONT65_000989"
     CDS             32645..35830
                     /gene="carB"
                     /locus_tag="ONT65_000989"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR2328638.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35884..36660
                     /locus_tag="ONT65_000990"
     CDS             35884..36660
                     /locus_tag="ONT65_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR2328639.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36657..37589
                     /locus_tag="ONT65_000991"
     CDS             36657..37589
                     /locus_tag="ONT65_000991"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR2328640.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37586..38302
                     /gene="pyrF"
                     /locus_tag="ONT65_000992"
     CDS             37586..38302
                     /gene="pyrF"
                     /locus_tag="ONT65_000992"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR2328641.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38309..38998
                     /locus_tag="ONT65_000993"
     CDS             38309..38998
                     /locus_tag="ONT65_000993"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR2328642.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38971..39240
                     /locus_tag="ONT65_000994"
     CDS             38971..39240
                     /locus_tag="ONT65_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328643.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39278..39919
                     /locus_tag="ONT65_000995"
     CDS             39278..39919
                     /locus_tag="ONT65_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2328644.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39962..40654
                     /locus_tag="ONT65_000996"
     CDS             39962..40654
                     /locus_tag="ONT65_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR2328645.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40651..41553
                     /locus_tag="ONT65_000997"
     CDS             40651..41553
                     /locus_tag="ONT65_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2328646.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41711..43417)
                     /gene="efbA"
                     /locus_tag="ONT65_000998"
     CDS             complement(41711..43417)
                     /gene="efbA"
                     /locus_tag="ONT65_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR2328647.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13953 a         7685 c         9473 g        12461 t
ORIGIN      
        1 attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg gattttctaa
       61 tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata aaaaatcatg
      121 taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac acaaaaaaga
      181 aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct gaggtgctgc
      241 tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca ctggaagaag
      301 caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg
      361 ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac tcagcagttg
      421 attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt gagcttccag
      481 gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca ttcgatctag
      541 ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg cttggcatct
      601 cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga taaacatcag
      661 aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa cctgtccaca
      721 cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac accccagaag
      781 aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc ttaccggctt
      841 gatataaccc agcgatttgc gcattcgtac cagttccttc agtgatcgta tcttttaaaa
      901 tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct ggctcataag
      961 tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg taataagtcc
     1021 ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct ccgtattttg
     1081 tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc gtactgtatt
     1141 cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca ttgaataatt
     1201 tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg gtgcccatat
     1261 attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac ggtgcgtcag
     1321 taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa tctgtgacag
     1381 gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg cctaaagtta
     1441 cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta ttggtgtcga
     1501 taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga tccgtgttga
     1561 cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt
     1621 ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct ttgacataat
     1681 tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca tccgttaccg
     1741 gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga agcatcgtat
     1801 acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga tcataggcgg
     1861 aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt
     1921 tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt gacatataca
     1981 ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc aatcttacag
     2041 ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg gtggagaaga
     2101 aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa
     2161 agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt ttataaaaac
     2221 ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa
     2281 ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt gtatatagtt
     2341 tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct tttgcgtaat
     2401 accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt attcctaaaa
     2461 ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa cctttgccag
     2521 aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag cttctagttt
     2581 gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata gaagtcaacc
     2641 gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc tagttcttcg
     2701 atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc
     2761 aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa caagacaaag
     2821 cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc atggaagttt
     2881 ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa gtaaaagcct
     2941 cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc cttgatcaca
     3001 gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg ctttttatgg
     3061 ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc ttcttcaaag
     3121 cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat tttttcgacg
     3181 ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat ctttaatcac
     3241 actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct gagatggata
     3301 agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt tttaaagaaa
     3361 atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat cagttagcag
     3421 aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc
     3481 aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt ttgtatcctt
     3541 tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac
     3601 aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct agtcagctaa
     3661 agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc tatgatgaag
     3721 aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa gcagatcatc
     3781 cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa taatcgatat
     3841 tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg ttattacgta
     3901 aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag acattttaca
     3961 acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg aattcttaga
     4021 caatatcatc aaagattatg aagcttacag caaagaactc cttgctttac aagaagaaaa
     4081 cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag ctcaaacacg
     4141 cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata tcttaaaacg
     4201 cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag caagtgcagt
     4261 caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct tagatgataa
     4321 tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg ggtaatcgcg
     4381 gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt
     4441 tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg aaaacaagct
     4501 aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc ggctggggaa
     4561 acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat ggtaggggcg
     4621 ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata gatggttacc
     4681 gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga aaaccgcaaa
     4741 catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa aaacatttga
     4801 aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact tttgtatcac
     4861 cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc tccttttttt
     4921 ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg caactgcggc
     4981 aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg aatgccaagt
     5041 tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag ctaatttatg
     5101 gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga
     5161 tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac tagatgcagc
     5221 ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg attgccaagc
     5281 gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt
     5341 accactgacg gaaactggcg cacattttca gttggaagta gctttgctga aagaccatgt
     5401 gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc gcttagaaaa
     5461 aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga caaattggcg
     5521 aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta ttgaagcagc
     5581 cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg aatcttggga
     5641 ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa aagcagatta
     5701 tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg aaattgccaa
     5761 agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac aagcagtcaa
     5821 agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt acggagaacg
     5881 tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg tattccgtcc
     5941 attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg aattttacgg
     6001 acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg atctattcca
     6061 gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt actaattgat
     6121 tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt tacaagaagt
     6181 aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata cacaaacagg
     6241 tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt acagcattta
     6301 tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt ctgaacatcc
     6361 ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag agtatgattt
     6421 ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt cttcagcaca
     6481 cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac acgcattgaa
     6541 tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg aaaagacgaa
     6601 ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc ttgataaggt
     6661 atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag aaaaagggac
     6721 tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga agagatttgt
     6781 agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag atgacactgt
     6841 ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac gatggaacga
     6901 aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg gtatgtatga
     6961 acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat caatgggtat
     7021 ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca gcttctggaa
     7081 aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata ttccttttga
     7141 caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg aggcagacag
     7201 tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga
     7261 aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag agtatctggg
     7321 aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt caggtgcaag
     7381 ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat tacttcattc
     7441 tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt ctccaatcaa
     7501 aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa atcaattaat
     7561 catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata tgaaagacct
     7621 gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct gaaattcatc
     7681 taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat ggataaatta
     7741 caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg acatgggagc
     7801 gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga accgcaggca
     7861 gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga taaagtcgtt
     7921 aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt gccaatagta
     7981 gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc taaaaatctc
     8041 tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga tcgtatcgaa
     8101 gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca tgggcacccg
     8161 atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc
     8221 acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact
     8281 gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa
     8341 ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg gatcaaaaat
     8401 aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt cggatcattt
     8461 tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg ccgttcagct
     8521 ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat attgtaactt
     8581 gtgattcgga attggcagga gaacaccgtt tattcggaag aataggactg tgacaagact
     8641 tttgtcacag tccttttttc gaattcaaca agtaaaactt ttaagaaata ttcagagagt
     8701 aaaattaaaa attgctgata aacgaagatg gaaattttat gtatttttat tggtctatgc
     8761 aataaataaa ttaaatattc agaatacaat tgattttaac gaaagggctt ctatcaatgg
     8821 ttgtgttgta tttatctgcc caggtagagt aaaatagatc attatctaaa aggtaattgt
     8881 tcatactata taagtcattt gaaagtaaaa agatactgta aataaaaaat gtggttaaat
     8941 aattctcata atcagtgtaa gcgttatcaa aaatatagca tacttgcttt gtagatgaat
     9001 gacacgatat atgtttggta tgttctgcaa gacagatgaa tatggaggtg gagacaatgt
     9061 cttccaaaga actaacaatg tatttagaga aagaattgga tcaactgaaa gaaaaggggt
     9121 tatacaatac gatcgacata ctagaaagtg aaaatggtgc ttgtatcatc gtagacggaa
     9181 aaaagatgat caatctagca tccaacaatt atcttggatt tgccaatcgc gaagaattga
     9241 aaaaagcatg tattgaagca accgaaactt atggtgtagg agcaggagca gtccgaacga
     9301 tcaatggttc gctgaaaatc catcagcaat tagaagaaaa aatcgcagaa ttcaaaggga
     9361 cagaagcagc aatcgccttt caatctggat tcaactgcaa tatgggcgct atctctgcag
     9421 taatgacaaa agaagatgct atcttatcgg atgaattaaa ccatgcatct attattgacg
     9481 gttgccgctt atccggagca aaaatcatcc gaattaaaca tcaagatatg aaagaccttg
     9541 agaaaaaagc aaaggaagcg atagaaagta agaaatataa aaaaatcatg tatatcacag
     9601 atggtgtttt ctccatggat ggagacattg cacgattgcc ggaaattatt ccgattgttg
     9661 aaaaatatgg actgatcacc tacgtcgatg atgcgcacgg ctcaggcgta actggaaaag
     9721 gtgcaggaac cgtcaagcat tttggattaa gcgacaaaat cgatatgcaa atgggcacgt
     9781 tatcgaaagc tataggtgtt gttgggggtt atgtagctgg atcgaaaaca ctaatcgact
     9841 ggttgaaagc tagaagtcgg ccattcctat tttctacttc gctaactcca ggtgcagctg
     9901 cagctgcatt ggcttcgatt actttgatgc aagaacatcc ggaacttgtc gaaaaagttt
     9961 gggaaaatgc taattatttt aaagaagaat tgaaaaaagt aggctataac atcggtatgt
    10021 ctgaaacacc tattactcca gtcattttgg gagacgaaaa agtgactcag actttctcta
    10081 agaagctgat cgaacatggg atttatgcta aaccaatcgt ttatccaact gtgcctcttg
    10141 ggacaggcag gatcagaaat atgccgactg cggaacatac gagggaaatg cttgatgaag
    10201 cagtagcagt ttatcaaaaa ataggaaaag aaatggaaat tatttaacta aatttagttt
    10261 tttcgtctat ttaaaattag agaacatgtt cacgcatata ttgttaatca tctgaagata
    10321 gtagtaaaaa agatcaggga ggatcatcat gaaaaaagta ttggtgacag gatgtttagg
    10381 acaaattggt tcagagttga tcacacggct acgtcaagaa ctaggcagtg aaaatgtcat
    10441 tgcgacagat atacgcatgc ctgaagtcat tgagaaaaac gatatctttg agatcctaga
    10501 tgtaatggat tacgaaaaaa tgcgcagcat agcagaacaa tatcaagtgg acacattgat
    10561 tcatttggct gctcttttgt cagctgttgc agaagcaaaa ccacaattag cctggaattt
    10621 gaacgtgact gggctggtca atgcattgga agtcgctaga gaattccagc tgaaattttt
    10681 tacgccaagt tcaatcggcg cgtttggtcc aaatactcca aaagatcata ccccacaaga
    10741 tactcttcag cgtcctacaa ccatgtatgg ggtaacgaag gtagctggag aactactatg
    10801 tgattattat tatgtgaaat atggcgtaga tactcgtggt gtccgtttcc ctggattgat
    10861 ttcttatgca accttgccag gaggcggtac gacagactac gcagtcgata tttattattc
    10921 agcaatcaaa gaaggaaagt acactagttt cattgatcaa ggtacatcaa tggatatgat
    10981 gtatatgcca gatgcgatcg atgccattat aaaactcatg aatgcagatc ctgatcgatt
    11041 ggttcatcga aatgccttca atatatcggc gatgtctttt gacccggaag agatcaaagc
    11101 atctattcaa aaaatcatac cagattttga aatggattat gatgtggatc ctgtacgtca
    11161 gtcaatagca gaatcttggc caaattcact agatgtttct tgtgcgcaag aggaatgggg
    11221 cttttcgccg aaatacgact tagatgtgat gacaaaggat atgttggaaa aactgaaaga
    11281 aaaattggta gagacggtat aaaacaagaa ccacatccaa aaagtttttt atacttttgg
    11341 gattggctat ggtatgaaag gggacaaggg atggaagaat atcaagccac gccagtaaaa
    11401 gaagtaaaaa tagaaaatga attaaaacgt acgatgggat tttttactgc tttatcgact
    11461 gttatgggca cagtgatcgg tgcaggggta ttttttaaag ccgcaagcgt agcagaagtt
    11521 acaggatccg ccagtttgca tatgttttct tggtttctcg gtggtatgat ttctgtctgt
    11581 gcaggactaa caggagctga actggcagct gctattccag aaacaggagg gatgattaaa
    11641 tatatcgaac ggatttacgg aaatacagca gcttttttat tgggctgggc gcaagtcgtg
    11701 atttattttc cagcaaatgt agcagccttg tcgatcattt tcggtacaca gttcgtcaat
    11761 ctatttggtc tttcccagtc aatgattgtt cccgtagctg ttacagctgc tgtatcgatc
    11821 ctgttgatca attttttagg ttctaaagct ggaggggctt ttcagtcgat tacgcttgtc
    11881 tgcaagctta ttcctttgtt tgtgatcgtc attttcggtt tgttccgaca agaaggagta
    11941 gacttccaac tgtttccgat ccaagcagga gaaaatcttt catttttctc agccttggga
    12001 gcgggcttgc tagcaacgat gttcgcatac gacggttgga tacacgtagg aaatatttcg
    12061 ggagaattga aaaagccggc gaaagatttg ccaaaagcaa tttctttagg aatcatcgga
    12121 atcatgatcg tttatttatt agtaaatgcg gtgttcttaa gaaccgcatc tatcgacgga
    12181 gtcgcaggaa acagcaatgc ggcaagtgac gttgcaaaaa tgatttttgg cggcttcggt
    12241 ggaagactgg tcacagttgg tatcttgatt tctgtttatg gaaccatcaa tggctatacc
    12301 cttactggaa tgcgtctccc ttatgttatg gcaaaagaaa acaacctgcc ttttagcaag
    12361 ctctttgcta aacttcatga taaaactaaa gttcctgtag ccgcaggtat cttagagcta
    12421 gtcatcgcta ttggcatgat gatgatcggt ggttttgata cactaacgga tatgctgatc
    12481 tttgtcattt ggatttttta tacgatggtc tttgttggtg tcattcttct tcgtaaaaaa
    12541 gaacctgatt tgtttcgacc atacaaagtt ccaatgtatc cgtttattcc actagtagca
    12601 atcattggag ggacttttat cgtatcaagc acgcttatca ctcagacctt tttagcttcg
    12661 atggggatag tattgacact tgctggtatc ccgatttatc tctatttaaa acgtagatag
    12721 ctaaaacatt cgaattttat tagaactgca cttctctgcc aaacaaaata ttttatttaa
    12781 atgaggagta cgtggaagaa atgtcaaaag tactcgttaa ttttgatcac gtaaatgtcg
    12841 ttttgtctac gatgaaatag caaagtaaaa gacagaaatc gggctcaacc gatttctgtc
    12901 ttttactttg atttgcgtaa ctgaactatt cggcgtacgc cgcgataaat atttaataaa
    12961 accaagcaac cagaaatgaa aatgggaagc caaatcagat agacagggta gccaagcact
    13021 ttagacaaag catgtggagt cattttatcc atttcaagaa gtgcttgctg ccaagtctga
    13081 agattgaaga ctagcatgaa acccgaaata atcaggagaa taaatcctaa ataatgaatt
    13141 gtagttcttt ttcttacata agcgatcgta gcataaagga acagtaaaaa aataggtacg
    13201 ccaaaaacaa agtacatatt cagcatctcg acaccccctt tgtgtttatc ttactttact
    13261 ttgaaagaaa tgagaaataa tagcctcgtt acttgcttga aacagcaaag gactgctaag
    13321 ataagagaga tacaaagaat caaggaggag tcatatggca agttatatcg aaccgattcg
    13381 ttttgcgctg attgtttttc catttttagc cttagcgatc tcatctgttt tttttattta
    13441 tgaatatcga aagtatggaa catttctagt aacaagggca gttattttgt attctttcgt
    13501 tttttattta ttgtgtgcgt attttttggt gatcttacca cttccggcaa aggagacggt
    13561 tgcccaaatg acagggccaa agatggagct gcatttattt gcttcatggc aacatttcag
    13621 agacaacacg gtattggtct tgtctgaacc tagtacctac ctgccggcaa tgaagcagag
    13681 tgtatttttg gaaccagctt ttaatgtttt attactcata ccatttggca tttatttaag
    13741 atattatttc cggctttctt ttatgaaaac gatgattgct agtttctgcc tttctctatt
    13801 ttttgaattg acgcagctaa gtggacttta ttttatctac ccaagaccat accgcttatt
    13861 tgatgtgaat gatttagtga caaatacatt gggtggaatc attggttttg tcgttacgcc
    13921 gttatttaca tttatgcttc ctacaagaag acgaatggat gaactttctt atgaaaaagg
    13981 aaagactgtc tcattgactc gacgcttggt cgcttatttg atcgactgga catttttatc
    14041 tcttattaat ttggtagcgg cgattttctt taaaattgcg ttcgattcag gggcagttac
    14101 ttctcatatc tggtggcttt tattagaagt aggggtatac tttatgattg taccgtttat
    14161 aaccaatgga tggacacttg gcaaaaaaat tgtgcgtatc cgtttggtgg aagaagaccg
    14221 cgaacgaatt tctttaaaag cattgatgat ccgctacggg tatctgtact ttctttttta
    14281 tggtttaagc tggttggggc agttgcttca gcaaatcatg aattcgacaa cgaatacggt
    14341 gttgcagatg agtgcagtat tcatttctct cggtgtcttg ttactacaag gtatttttat
    14401 tttaaatatc ttgctatcat ttttgagaaa aaaacggatt ctgttttatg aaaaagctag
    14461 tcacacatat actgttagca cgattggcca aacaaaggat gatgattctc gaaattagct
    14521 gagcaaaaac gctttgtctg tgttataatg ccaaagtcgt gataatttaa agagaagagg
    14581 tgcacgaatg aataattttg atatattccg cttcaaaccg tttatcaatg aagcgttaac
    14641 cgcaaagggc ttcaaacagc ccacagaggt ccaggaacgt ttgattcctt taattgcaaa
    14701 aggaaaaaat atcattgggc aatctcaaac tggtacagga aaaacacata cgtttttgtt
    14761 gcctttgttt gaaaaaatca atgcagaaaa agaggaagta caagttgtta ttacagctcc
    14821 aagtagggaa ctagctgcac agatctatga agcagctaca caaatcgcta aattttctac
    14881 accggagatc cgtgtctcga attttgtcgg cggtacagac aaacagcgtc aattaaatcg
    14941 tctgcatcac caacagccac aggttgtagt aggcacgcct ggtagaatat tagacatgat
    15001 gaatgaacaa gcgctgaaga ttcacaccgc atttgcattt gttgtcgatg aagcggatat
    15061 gacattggat atgggttttt taaatgaagt agaccaaatt gcaagtcgat taccagaaaa
    15121 gctgcagttt ttagtttttt ctgccactat tccagaaaaa cttcgtccat tcttacgaaa
    15181 atacatggaa aatccaatca ttgaagaaat caaaccaaaa gcagttattt ctgaagacat
    15241 tgaaaattgg ctgatttcaa caaaaggtaa agatgagaat caaatgattt atcaactgct
    15301 gacaattggt catccttatt tagcaatcgt atttgccaat acaaaacagc atgtggatga
    15361 aattgccgat tacttgaaaa accaaggctt gaaagtagca aagattcatg gagacatcac
    15421 cccaagagag agaaaacgag tgatgcgaca agtacagaat ctggagtatc aatatgttgt
    15481 agcgactgat ttagctgcta gagggatcga tatcgaaggt gtctctcatg ttattaatgc
    15541 agaaattcca gaagatctag acttctttat ccatcgtgtc ggacgtactg gacgtaatgg
    15601 cttaaaagga acggccatta cgttatattc gccaaatgat gagcaagcca ttgatgcgat
    15661 agaaaaattg ggcgtctcat ttgaaccaaa agaaatcaaa aacggggaaa tcgtcaaaac
    15721 atacgatcgt aatagacgtg ccaaacgaga aaaatcaaaa gatgtattag atcccacgct
    15781 gattggtctt gtgaagaaga aaaagaaaaa gatcaagcct ggttataaga aaaaaatcga
    15841 ttgggcaatt gcagaaaaaa ataaaaaaga acgcaaaatc gaacgcagac agcaaagccg
    15901 tactgctcgt aaaaataaga aaaattcaca taaataaaaa acaagaggtt gtgacaaaag
    15961 gcgtgtcaca atctcttgtt tttaagctag actttttttc attccgatac aaagtacttc
    16021 atcttcaata aaaggttctg tgcaagtatg atacccttgt ttttcataaa aatctacagc
    16081 cgtcatttca gctactacat aactagtctg acaaccttct gaaaccgccc gtttttcagc
    16141 agcttcaagc aactgaccgc ctaacccttt tcctcggtat tcttttgcta cgcaaaatcg
    16201 ttccggcttg aagcaagtag gtgttttctt ttcataacgc aaagtagcta ctggtctttt
    16261 ttgatcaaga aatagataat aggtttgttg cgggatatct tttccgtcga attctagttc
    16321 tgcaggaatc ccttgttcta atacaaaaac ttgatatctc aaaaagcagc tagctgcgcg
    16381 tatccattca tccgctccaa ctattttttt catcatattc ctccttgtca tgaatagcag
    16441 agatgtattg attcctttat tcttatcgta ccaaaaatcg tggagtatga ccaaaaggaa
    16501 atgaaaataa aaattgagat attgacagtg atcaatagaa tgcgtatact aattaagtac
    16561 gaaaaggata tgatttgttt tttaagactc acagaaaatt ctttcttggc tgaaaaaaga
    16621 atagtcacta atcaaattgc tccctttttc tattttggta gaaataccga cgcagctcag
    16681 cgttatcgag tttaagaggt aatgattaca catcattgca atcaaggtgg taccgcgagt
    16741 tttcgtcctt ggctgtgatg gtgttttttt gtatttaatt tatagaaagc aggtaatgac
    16801 atgaaacaat taagtagtgc acaagttcga caaatgtttc ttgatttctt ccaatctaaa
    16861 ggacatacga ttgaacctag tgcgtctttg atccccgtga acgatccaac cttattgtgg
    16921 atcaactctg gtgttgcaac attgaaaaaa tattttgatg gttcagttgt gccagaaaat
    16981 ccaagaatca caaatgccca aaaatctatt cgaacaaatg atatcgaaaa tgtcggtaaa
    17041 acagcacggc accatacgat gtttgagatg ctgggtaatt tttcgattgg tgactatttc
    17101 aaaaatgaag ccatccactg ggcatgggaa tttttgactg atccaaaatg gatggcattc
    17161 gatccagaaa aactttatgt aacagtttat ccaaaagata cagaagcaaa acgcatctgg
    17221 catgaagaag ttggcctgcc actagatcac attattgatg tagaagacaa cttctgggat
    17281 atcggtgcag gcccatgtgg tccggatacg gaaattttct atgaccgtgg agaagcttat
    17341 aacgatttag cagaagacga tcctgaaaac tatccaggag gagaaaatga acgttattta
    17401 gaaatctgga acttagtatt ttcagaattc aaccataagc ctgataacac atttgaacca
    17461 cttcctcata aaaacattga tacggggatg ggattggaac gcatggtctc aatcgttcaa
    17521 gatgcgtcaa cgaactttga gacagacttg tttatgccta ttatccacgc cgtagaaaag
    17581 ctaagcaaac aagtaaaata tggtgaaaac ccagtaacag atatttctta caaagtaatc
    17641 gcagatcata ttcgcgccct ttcatttgct attggcgacg gtgcattgcc atcgaatgaa
    17701 gggagagggt atgtcttacg ccgtttgctt cgtcgtgctg tcatgcatgg gaaaaaatta
    17761 ggtattgaac aagcattctt atatcgttta gtccctgttg ttggggaaat catggtaagt
    17821 tattatcctg aagtacttca acaaaaagaa tttattgaaa aagttatccg tacagaagaa
    17881 gaacgtttca atgaaacaat caatgaaggt ttggaaatct taaatcaagt gatccaagaa
    17941 gtaaaaaacg aaaacgggac gactctagca ggaaaagaca tcttcaaact atacgacaca
    18001 tatggcttcc cggtagaatt aacggaagag gtggcagaag atgaaggact gaaagttgat
    18061 catgctggtt ttgaaaaaga aatggatgca caacgcgaac gtgctcgctc tgcccgcagt
    18121 actgaaaaat caatgggggt acaatctgct ttattgacag atatcaaagt agaaagtaaa
    18181 ttcattggat acacagaaca agaatcagat agtcaattat tggtcatcgt taaaggtgag
    18241 tctcttttga atgaagtcgc agaaggtgaa gctcaaatga tctttgatca aacacctttc
    18301 tatgcagaaa tgggtgggca agtagcggat caaggatgga tcatgaatga agtaggcgaa
    18361 atcgtagcag ttgttacaga tgttcaaaaa gcgcctaatg gtcaatttat gcacaccgta
    18421 gaagtaaaag caccaatgag tgaaggacaa acctatcatc tattgatcga tcaatcgatg
    18481 cgcaatcgga tcatcaaaaa ccatacagca acccatctac tgcacaaggc attgaaagaa
    18541 gtacttggcg agcatgcaaa tcaagctggt tctttagtcg ctcctggaca tttacgattc
    18601 gactttaccc actttggtca aatcacttca gatgaattag ctgaaatgga aagaatcgtc
    18661 aacgaaaaaa tctgggaagc tatcccagtt gaaacaattg aaacagatat cgatacagcc
    18721 aaaaacatgg gtgcaatggc tttgtttggt gaaaaatacg gacatgacgt tcgagtagta
    18781 aatattggcg gctggtcaat cgaactgtgc ggaggcacac atgtagcaaa tacagaaaac
    18841 atcggaatct tcaaaatcgt ttctgaatca ggaattggtg caggcgtgcg acgaatcgag
    18901 gctgtaacaa gtaaagaagc gtacgaacta cttgaagaag aagaaaaaca actaaaacaa
    18961 gtagcaacaa ttgttaaatc acctcaattg aaagaagttg tttcaaaagc ggaacaattg
    19021 caacagcaag taagagagct acaaaaggaa aatgaacaat tagctagcaa gttagcgaat
    19081 caacaagctg gtgatgtatt caaagatcca gaaacgatca atggaaaaac aatcattgca
    19141 gcacaagtaa aagtgaaaga tatgaatcag ctgcgtcaat tagctgacca atggaaacaa
    19201 aaaaatattt cagatattct tgttttagca aacgaacaag aaggaaaagt cagtctgcta
    19261 gcggcaatga cgaaagaatc aaatgaagct ggactaaaag ctggcgactt gattaaagca
    19321 atcgcaccga aagtaggcgg tggcggtggt ggacgtccag acatggcaca agctggcggg
    19381 aaaaatcctg ctggtattcc agatgcactt aaagctgcga aagaatgggt tgcgcaacaa
    19441 tagggcgttt acgtttgaca gaaaaacaaa agttcaaaca ttgaacgaaa catgattatg
    19501 ggggcgaaga caaacaagtc ttgccctcat acttttttca attggatggc gcatctaatc
    19561 aaatggtcgg attcggatgg tctttagttg acgaaagtga cagaaatttt tacgattagt
    19621 ataaataaaa aggggtgaaa aaatgaatag tagaaataag acctttcgac tagtcttacg
    19681 tgcgatcttg ttagcaatca tcatcgtaca agcgatggtt ccttggctgg ggtttattcc
    19741 attaggattc attagtttaa caattatcca tatcacagta attattgctg cagtagtatt
    19801 aggcccaaaa gatggcatgg tgattggctt gttttgggga atcgctacaa ttgttcgtgc
    19861 ctatgccatg ccaacaacgc catttgatac attagtgttc acgaatccga ttatctcggt
    19921 agttcccaga gtacttgttg gcttagtagc aggcttggtt ttccactgga tttatcaaaa
    19981 aaataaatca ataacgatca gctcagcctt tgcaggggta ctgggttcat tggtcaatac
    20041 agtcttagtg ttaggattca tgggattatt ttatacaggt gcaacggcag atgcctatgg
    20101 tgtagactct gctcttttat ttaaaacctt agcaggggtt gcagcaatca atgggatacc
    20161 agaagcaata ggtgcgggaa tcataacacc attactagca aaagcgttat ttgcagcaac
    20221 accgttgaaa ccagaataat gaccatcttt taatagtaat aggacgcacg atagatagaa
    20281 aaaggaagag aggagaggaa aacaatcgtc ctctccatct ttttacctga gcaacaagca
    20341 aactagaagg ggtaaaaaac ttggctaaga aacaagaaga aaaaaagaaa caaataccgc
    20401 aacagcggac aaaaaaagga gtactagcgg gtatcttagg attggttacg aatctgatct
    20461 tgtttgtctc taagctaatg atcgggcttt tttcaggaag tgtctccatt atggctgatg
    20521 cagtgaacag tctatcagat actgcttcat ccatattgac tttgatcgga tttaaaattg
    20581 ctgcaaagcc agcagatcag gaacatcctt atggacacga acgcttcgaa tatatcagtg
    20641 ggttatttgt ttcgatcatc attacttatg taggatttca atttttagag tcttctgctg
    20701 aaaaaatcat ccatccagaa aatgtctctt taacacccat cgtctttttt gtcctaatca
    20761 tttccatctt tttgaaattt gcacaaggca aaatgtatca ggttattgcg caatccattg
    20821 attctgaaac attgagagct acagcaacag atagttataa tgatgtcttt actacactgg
    20881 ctgtattgct ttcagcagca atcgaatggg taactggatg gcgtattgat ggatatatag
    20941 gttttgcttt agcattatat atcctttata gtggattgac gatgcttcga aattttgtat
    21001 acgaactatt aggaagtcgc ccaacgccag cagagattga agaaatggaa gagcatttaa
    21061 atgcttgcga tgatatactt ggctatcatg atttattggt gcataactat ggacccaata
    21121 aacgctttgc atcagtccac atcgaagtag atgaatcaat gaatttgaat gtggctcata
    21181 agattattga tctgatagaa aaagacttta aaaagtcttt ggatgtggaa cttgtctgtc
    21241 acttggatcc aatgcctgtt gataacgaaa aatactataa tatcttgcgg cagttaaagc
    21301 aaattcttct tgaaacaggt acagggctta atattcatga ttttagagta attcatcaag
    21361 gaaaaacgct gcaatttgat gtagtggtcc ctgagaaatt ccagtttgtt gatacagaac
    21421 tagaaagaat cttacgtagt caaatagaag aaaaaatcgg agattaccaa ttggaaatta
    21481 cgtttgatca caattatctt ttatattaaa tgagaaacta taaaaagact ccttaaagtt
    21541 tttaatttaa agagtctttt tttcttttaa ttgtttatgg agagcctgac gtgcaagatt
    21601 atctgctcct ttatttttgt tttcgggcaa ccattgcagg atcaataaag aaaattcgga
    21661 tagaagttct tggattcttt gaaggtaggg aaggaagagc tcgttcttcg ttgcgttttt
    21721 gtcgaccgta gaaactagtg ttttgctgtc tgaaaaacag aaaatcgttt cattctgtaa
    21781 gtcgtttttc tttagaaatt ctaaagtttt taaaaagact tcgaactctg cttgatggtt
    21841 tgtccctata gaaagcggga tagatagctg ttcttgttta ccttgataga caatcaagat
    21901 tccacctcca ctaggaccag ggttgccttt tgtagaggca tcaatgtata cttttaacat
    21961 aaaaagagat tctccttttt gttcaaacta atgttatggt aagattatat cattactgaa
    22021 tcggtgagag gttagagaat gaaaaaaaat attcgatggc agcctgaact ggcacatacg
    22081 atcatctatt ggtcattgac cttgatcatt ctgtttttta gtctgattct ttctttagaa
    22141 aacacaggtc cttattggaa aagcaatttt gttttaggta tttttcttgt ttttctattt
    22201 ctaggtttgc ggcgttcttt tattttaaaa tcaaactctg tgattatacg ttatgctgct
    22261 ttttggaaag accgagaaat atctgtcgga cagattcgat ctttgacgcc aactaatact
    22321 ggggtgcaaa tgatgttgca agatgagaaa gccccatctg ttttcatgat gcgaaaaaaa
    22381 gatcaagtag tcctttttga tcattttgaa aaaaacggac ccaaatcaga atcaattcct
    22441 tatccttata cgaaaaaaag aaaaagctaa ttttaaaaat cagaacctaa aaaaacgaat
    22501 ttttacagga aaagaaaaga gtgagacgta cggttcatcc cgtttagcgt ctcactctat
    22561 ttgtttatct attcatgcat actagtatcg gtagtctgaa cgttgccttt cttgacatgg
    22621 gcagcttgtg ttcctcgatt cccttcgatg atctcaaatt cgaccacttg attttcttcc
    22681 aaagttttga atccctcttc ttcaatcgca gtaaaatgta caaaaatttc ttctgtgtca
    22741 tcataactga taaagccgta tccttttttg ttgtcaaacc attttacaat tcctgtggtc
    22801 aaagcactca cgacctttct aaagtctgat catagccttc attataaaga gtatttataa
    22861 gtacgtcaaa taaattaaaa acctgtaatt ttatgttttt tttctgaata aaaaaaggta
    22921 taattggcat atcttaacaa agaagatatt ttgttgaata aagttcgtga aaaggggtaa
    22981 tcaaatgaag aatgatctgc aaatttcaca agaaacagag ctagaaccga ttgttgaagt
    23041 ggctagtaaa ttagatctta aggaagacga tttggaactg tatgggaaat ataaggcgaa
    23101 aatcaatttt tctggaatca atcgatcaaa ggataacgct gaggggcatt tgatcttagt
    23161 tacttccatc aatccaacac cagctggcga aggaaagtca acgatcactg tagggttagg
    23221 agacgcgctc aataaaatca ataaaaaatc tgttattgca ttgcgagaac cctcacttgg
    23281 tcctgtgatg ggaatcaaag gtggtgctgc aggcggagga tatgcacagg tattgcctat
    23341 ggaagatatc aacttgcatt ttactggtga catgcatgct atcacaacag ctaataatgc
    23401 attatctgct cttttagata atcatattca tcaaggaaat gaattgaaca tcgatgcccg
    23461 ccgagtgatt tggaaacggg tagtcgatct caatgatcgt gaactgcgta aagtaattgt
    23521 tggcttgggt gggccaatcc aaggtgttcc aagagaagat gggtttgata tcacagttgc
    23581 cagcgaaatc atggctatct tgtgtcttgc ggcagacctt gaagatttaa aggctcgtct
    23641 tgcaaggatc gttgtcggct atacatttga ccgtcagcct gtgactgctg gagacttgaa
    23701 agcagaaggc gctctggcat tactgctgaa agatgcaatc aagccgaatc ttgtacaaac
    23761 aatttacgga acaccagctt ttgtccacgg gggaccgttt gccaatatcg ctcatggctg
    23821 caatagtatt ttagctacca agacagcatt gagattagca gattacgttg tcacagaggc
    23881 aggatttggt gcagatcttg gcggagagaa atttttagat atcaaagtac ctaacttgaa
    23941 aaaagcgccg gatgctgtgg tgattgtcgc cactgtccgt gcattgaaaa tgcatggcgg
    24001 aatgaaaaaa gatgaactta aaaatgagaa tcttgatgct ttgaaagtag gttttgcaaa
    24061 tttaaagcgc catatacgta atatggaaca atatcagcta cctgtgatcg tcgcaatcaa
    24121 tgaattcgta acagatacag acaatgaact cacactgttg gagcacttgt gtgaaaatca
    24181 ggggattctt gccaagagag cgagtgtatg ggcagatgga gcagaaggcg gcgttgattt
    24241 agcaaaagct gtagtacgat tgattgatag aaaagaagct aattacaagc cattataccg
    24301 cctagaagaa acgatacaag aaaagacaga aacaatcgtc aagaaaattt acggaggaaa
    24361 tggtgttgtt ttcagcaaaa aagcgcaaaa acaaatagaa gagtttacga agaatggatg
    24421 ggacaaactg ccgatttgta tggcaaaaac acagtattca ttttctgatg atccttcact
    24481 gctaggtgca ccggaagatt tcaccatcac gatccgcgaa atcatcccaa aattaggggc
    24541 aggattttta gtagccttga ctggagatgt tatgacgatg cctggtttgc ctaaaaaacc
    24601 tgctgcattg aatatggacg tgacaaatga cggagaagtg ttgggattgt tttaagcttg
    24661 attataatct accatcgaag gagctgtatt ttgactacag cttctttttt tagacgatag
    24721 ggaattaatt ttgtttacac taggatcatt ctttttcttt attgtatttt gagctatact
    24781 aaagcataat aggagggtat gatgaaaacg acatttataa gaaaaaccgg tttttacggt
    24841 atgggtagct cgattcagct aagaatcaac aatgaaaaac gcagtgttct cagtcatgaa
    24901 caaaagataa cgcttgatct ggaagtgccc tttacgatgc aggtcacctt ttattggcta
    24961 aaaagccccg tttatacagt gaaagaagca aagcctgctt atgtgatcac aatgaattac
    25021 ttgattcttc aaatctatcc gtttttattt ctagcaactg ggttgggggc cgttttccta
    25081 caaaatttat ttttcagttt ttttgcagtt attgtgttga ttgcgttctt tttctatata
    25141 aaaaaccggg catatttgat caaggaggaa aattatggca aactctgatc tttttttaat
    25201 cagtttcaat cgaatcgaaa aatggatgag agacgagatg ggaaatgcac gaaatatggg
    25261 atttaccgaa cttgtcagac gcctagctca aagaaaacaa ttaatgatca gaaaatacga
    25321 agatgatctt ttacagcttg ctcagttaag gaatgcgatc gtccatgatc ggatcgcagt
    25381 cgattttgtc attgcagagc caaatgattg gacagtcaag cgaatccagc tgatcgaaca
    25441 agaactgatt cagcctgaaa ccgtgctgcc tcgatttgca aaacgtgtaa caggttttga
    25501 acaagatctt ccgttacttg aactgttaaa gatagtagca gaaaaaaggt attcacaatt
    25561 tccgttatac aataaaggaa aatttgtagc attgattact ttacgtaata tcgggttttg
    25621 gctagcaaaa gaaagccaaa aaggtccggt tgatttaaca aataaacgag cgttggattt
    25681 gatcatccaa aacggaaagt acacaaatta tcattttgtt ccagctaaaa cacatattta
    25741 cgaggttgaa gcgatgtttc gtgagcaagg gactttggaa gccgtactta tcactaaaga
    25801 tgggaatccc gatggtaatc tgctcgggat cgtacgacct cgtgatattt ataaacaaat
    25861 agaaaaggat tgattttttg ttaattattt attggatatt cagcgctttg ctagtaggag
    25921 tggatcagtt ggtcaaatgg tggatcactg ataatctagc gcttggagag acgaaaaatc
    25981 ttataccagg tattttgtca ctgaatcata ttcgaaacac tggagccgca tggagtatgc
    26041 tagaagggaa gatgtggttc tttactatag tgacactcat tgcagtcgtt gtgattctca
    26101 cattgatgat taaaaatcgt gaaaaaggta atcgctggtt catgattgga ttgagtttga
    26161 ttctagctgg tgcgattggc aattttatcg accgtattcg tcttggctat gtagtagata
    26221 tgttccagac agattttatg agctttccga tttttaacgt agcagatgtg acacttgtat
    26281 gtggagtgat ttgtatgtta atttatatta ttttagatga aaaggatcaa agaaaaaaat
    26341 gacagaactt aaaacgacga ttcaccatga atctggtagg atcgataaag tattgactaa
    26401 tttattcacc gattacagtc gttcgcaaat tcagctgtgg ctgaaaaatg gagcggtttc
    26461 tgtcaatgga cagccagtca aagcaaatta caaagtaaaa caaggagacg agattactgt
    26521 cttgatcccc gaaccagaaa cattagatgt ggtcccagaa aatatcccgt tgacgatcgt
    26581 ttacgaagat gaagatgtag cggtgataaa caaacctcaa ggaatggtcg ttcatccttc
    26641 agcaggtcat acgcatggca caatggtcaa tgcgctgatg taccatatca aggatctttc
    26701 tacgatcaac ggcgttattc gtccaggtat tgttcaccga atcgacaaag atacttccgg
    26761 actgctaatg gttgccaaaa atgaccaggc acacgaatca ttagctcagc aattaaaaga
    26821 taagacctct ttgcgcaaat atgttgcttt agtccatgga gagattcctc acgaaaaagg
    26881 aaccatcaat gcgccaatcg gccgttcgaa ggaaaaccgg aaaatgcagg cgataagaga
    26941 agatgggaag tcggctgtca cacattttac tgtattggaa cgtttcgaag gatttactct
    27001 ggttgaatta aaactagaaa ccggaagaac acaccagatc cgtgtgcata tgaagtatat
    27061 cggttatccg ttagctggag atccagtata cggaccgaag aaaactttga aaggcaatgg
    27121 tcagtttctc catgcgcaaa tgttaggttt tacgcacccg actacaaacg aaaagatgat
    27181 ctttgaagca cctcttccag aagtttttga aaaaacttta gaaaaattaa gaaaaaacat
    27241 tgcattttaa tttagataac ggtatagtaa ttaacaacga aagcataaaa cttaatagaa
    27301 ttcctttaag tttagtcccg tgaggctaag aagggtggga gcaaggatac gtatggtgcg
    27361 ccataagtag attgctcatt gacatagtat agctatcagt gtatcagtat acgctcagtc
    27421 taaacattcc tcctgtcctc ttagggcagg agttttttta tgcaaaaaag tagggggaat
    27481 gaaaatgcaa gcaaaagaag tagtagatca ggtaaccatg aagcgagcat tgactcgaat
    27541 tacttatgag attatcgaga gaaaccatag tatccaagat atcgtattgg taggtatcaa
    27601 aacacgagga atctacattg catcaagaat tgccgaacgc ctgaagcagc tagaagatat
    27661 cgatattcct gtaggagaac tggatatcac tttataccga gatgacaaaa aagaaaatcc
    27721 agaagaaccg gaacttcatt cttctgacat cccagtttcc ttagaaggaa aagaagttat
    27781 tttgattgat gatgttttgt atacaggacg aacgattcga gcagcaatgg acgcagtaat
    27841 ggattttggc agaccaagaa aaatttcatt agcagtcctg gtcgatcgtg ggcatcgaga
    27901 attgccaatc cgtgcagatt atgttgggaa gaacattcca acatctcggg cggaagagat
    27961 tttagtagag atgcaagagc ttgatggaca agatcgcatc atgattttga aagaggaaga
    28021 ttagaggaga gagaaacatg tcggagaaaa aaggatttca taatgatgac gtggtgttag
    28081 atatccacga tcgcccaacc ccaggacatt gggtaggatt aagtttacaa cacttgttca
    28141 cgatgttcgg agcaaccgtg ttagtcccca tcttagtagg tatcgatcca ggtattgcgc
    28201 tagtcagttc aggactaggt acattggtgt acttatttac tactaaagga aagatacctg
    28261 cgtacttagg aagcagcttc gctttcattg cagcgatgca gcttttgatg aaaacagatg
    28321 gttatccagc gattgcacaa ggagcagtca caactggatt agtctatttg attgttgcgc
    28381 tgatcatcaa aaagatcggt tcagcatggc tggataaaat cttgcctcca atcgtcgtcg
    28441 gtccagtcat catggttatc gggttgggac ttgcggcaaa tgcagcaaca aatgctatgt
    28501 ataatcaggg cacgtatgat ttcaagtata ttgctgtagc gctgatcaca ttaggattaa
    28561 caattttctt caacatgtgg ctaagaggtt ttctcggatt gattccgatt ttactaggta
    28621 ttattagcgg atacatcgta gccttgctgt ttggaatcgt ggatactaag ccaatcgtag
    28681 acgctgcctg gtttgctgtt cctaactttg agattccctt tgtacaatat gaaccaaagc
    28741 tatatcttgg agcaatcaca acaatggcac ccatcgcttt tgtgacgatg acagagcata
    28801 tcggccatct tatggtattg aataaactaa ctaaacgtaa tttctttgaa gaaccgggtc
    28861 ttcataagac gttgacaggt gatggattag cacaaatcgt ggcagccttt gtcggaggtc
    28921 ctcctgtaac aagttacggt gaaaacattg gtgttctggc tatcacacgt gtccatagcg
    28981 tgtttgtgat cggtggagct gcagtatttg cagtcatttt aggattcgta ggaaaattaa
    29041 gcgctttgat tttaagtatc cctggaccag tcatctcagg aatcagtttt atcttatttg
    29101 gagtaattgc cgcaagtgga atgaagattt taatcgagaa caaaatcgat tttgataaaa
    29161 agaaaaatct gttgattgct tcagttatct tagtagtagg aattggcgga ctcgtctttg
    29221 aagtaggcac atttacattg tcggcaatgg cattagcaac tgtacttggc atcattttaa
    29281 atcttatctt accagaaact tcacgaagcg aagaaaacta aggaggaaac attatgatca
    29341 tcaaatcaga acgtatcagc ttaaagcatt tattgaccgt tgaggcactt actgatcaag
    29401 aagttatggg cttgatccgc agagggcagg aattcaagca aggtgctaat tggactccgc
    29461 aaaagcgcca gtactttgct acaaacctct ttttcgaaaa tagtactcgc acacataaaa
    29521 gcttcgatgt agcggagaaa aaactagggc tagaagtgat tgaatttgaa gcaagcacaa
    29581 gctctgtcca aaaaggggaa acactatacg atacagtact gacgatgtca gcattaggcg
    29641 tagacgtagc ggttatccgt catggtgatg agaactatta tgacgaactg atacaaagta
    29701 aaacaatcca gtgttcgatt atcaatggag gagacggaag cggacagcat ccaacccaat
    29761 gcttgcttga tttgatgacc atttatgaag aatttggtca ttttgaaggt ctgaaagttg
    29821 ccatcgtagg tgatatcact cactctcgcg tagctaaatc caatatgcag atgttaaaac
    29881 gtctaggcgc acaagtattc ttctcaggtc ctagagaatg gtacgacgat gaattcgaag
    29941 tgtacggaca ttatatgcca ttggatgaac tactagatca agttgatgtc atgatgatgc
    30001 ttcgggtgca acacgaacgc catgatggaa aagaaagttt ctcaaaagaa ggctaccatc
    30061 aggaacatgg gttaacagtt gagcgtgcga agaagttgca aaaacatgca atcattatgc
    30121 acccagcacc agttaaccgt gatgtagaat tagctgattc attagtggaa ggattacagt
    30181 cgcggatcgt tcagcaaatg agcaatggag tattcgtcag aatggcaatc ttagaagcag
    30241 tgctaaatgg aaaagcgtaa ttataggagg atgaaaaaat gaaaacactc attaaaaacg
    30301 gtcagatcaa tacacgaaaa aatatgacga caccagcaga aatttggata gaagatgggc
    30361 gaatcaaagc aatcggtaca ggattctcag aagccgaatt tgatgaagtg tttgatgcaa
    30421 agggacaatt gatcacacct ggtttagtgg acgtccatgt tcatttgaga gagccaggat
    30481 ttacatacaa ggagacgatc gaagctggga ctcgctcagc tgctcgtggc ggtttcacta
    30541 ctgtatgcgc gatgccgaat ttaaatcctg tacctgatac ggcagaaaag ctgagacaag
    30601 tatatgacat cattagaaaa gatgcagttg tgaaggtatt gcagtatgcg ccaatcacag
    30661 aaaatctgcg aagtgagaaa ttagttgatc aagaagcact gattgaagaa ggtgcttttg
    30721 ctttcacaaa tgacggtgta ggtgtccaaa cagctgggac gatgtatctg gctatgaaag
    30781 aagctgcaaa aaacaataaa gccctagtag cccatacaga agatgaatct ttattatttg
    30841 gcggcgttat gcacgctggc aagaaggcag aagaacttgg tcttcccggg atcttaagcg
    30901 taaccgagtc ttctcaaatt gctagagatc tgttgctagc tgaagcaact ggtgttcatt
    30961 accatgtttg ccatgtttct actaaagaaa gtgtccgtgt aatcagagac gcaaaaaaag
    31021 caggaatcca cgtaacagca gaagtttcac cccatcatct tattttgatc gacgaagata
    31081 ttcccgaaga tttcggcttt tggaaaatga acccgccgct aagaggacgg gaggacagag
    31141 aagctttgat cgaaggcttg cttgacggta cgatcgattg catcgctaca gaccatgccc
    31201 cccatggatt agaagaaaaa agccaaagtt tcatgaaatc accatttggt atcgtaggaa
    31261 gcgaaacagc gtttcaactg atctatactc attttgtaga aactggtcga ttcacgttgg
    31321 aacaagtgat caattggttg gcagttaaac cggctgagat ttttggtttg aatgcaggaa
    31381 cgctaacggt tggtgccccc gctgatgtag cagtttttga tattacacaa acttgcacga
    31441 tcgataaaga agacttttta tcaaaagggg agaatacacc ttttatcggc tggaaagtaa
    31501 aaggcgaaac acaaatgact tttgtcaacg gaaaacttgt gtggcagaag ggagaataaa
    31561 agtatggaac gtttgcttat attagaagat ggaacagttt tcgaaggaaa ggcttttggt
    31621 gcagatgcta gtgtcttcgg agaaatcgtt tttacaacaa gtatgactgg ttatcaagaa
    31681 actatcactg atcaaagttt taacggtcaa atcattacat ttacctatcc tatggtgggt
    31741 aattatgggg taaatagaga tgattatgaa tcgatttctc caacatgcaa aggagtagta
    31801 gtaaaagaac atgcacgtgt agcttcaaac tggcgaaacc aaatgacgct agattcattt
    31861 ttgaaacaaa aaggaattcc tggaatatca ggtatcgata cgcgcgccct tactcgcaaa
    31921 atccgtgaag tcggaacgat gaaagctagt atagttgatg ctggagattc cttcgaacat
    31981 gcatttgatc agctgaaagc aaccgttcta gcaaccaatc aagtgcagca agtctcaact
    32041 aacaaacctt atccaagtcc aggaacaggt cggaaagtca ttgtcattga cttcggcttg
    32101 aaacatagta ttttacgaga attgagtaaa agaaactgta acctcacagt acttccctat
    32161 aacacagatg ccgagacgat tttagcgcta tcaccagacg gagtcatgct gacgaatgga
    32221 cctggtgatc caaaagatgt gccagaagca atcgaaatga tcaaaaaaat ccaaggaaaa
    32281 gtaccgattt ttggtatttg tctcggtcac cagttatttg ctttagccaa cggtgcagat
    32341 acctataaaa tgaaattcgg acatcgagga ttaaatcacc cggtacgaga agtagcgacc
    32401 ggaagaattg attttacttc tcaaaatcat ggctatgcag tcgatgaagc cacgattgat
    32461 ccagaaaaac tcatcgtaac tcatgtagaa gtaaatgacg gaacagtaga aggagtcaaa
    32521 cacagagatt atcccgcttt cagtgtgcag tttcatccag atgctgcccc tggtccgcat
    32581 gatgcactac atttatttga tgaatttatg gaaatgatgg acgcaggaaa ggagcagtaa
    32641 acagatgccg aaacgtacag atatcaagaa aattatggtg attggatctg gtccgatcat
    32701 cattggtcaa gccgcagaat ttgactacgc aggaacacaa gcctgccttg ccttaaaaga
    32761 agaaggatat gaagtggttt tagtaaattc taatcctgcg acgatcatga cggataaaga
    32821 agtggcagat caagtatata tcgaaccgat cacacttgaa tttgtatcaa ggattcttcg
    32881 aaaagagcgt ccagatgctc tccttccaac tttaggcgga cagacaggcc tcaatatggc
    32941 aatggagctg gcagcatcag gtattttaga agaattgaac atagaacttc taggaacaaa
    33001 gctttctgcg atcgatcaag cagaagatcg tgatctattc aaacagttga tgaaagaatt
    33061 gaatcagcca atccccgagt ctgaaatcgt caatacagta gaagaagctg tagattttgc
    33121 aaataaaatc ggctatcctg tcatcgttcg tccagccttt actttaggcg gaactggtgg
    33181 aggtatgtgt gaaaatgaag aagaattacg tatcgtatca gaaaatggat taagtctttc
    33241 ccccgctacc caatgtttga ttgaacgaag tattgcaggt tttaaagaaa ttgaatatga
    33301 agtgatgaga gattcggctg ataatgcaat cgttgtctgc aatatggaaa attttgaccc
    33361 ggtgggtatc catacaggtg attctatcgt atttgcccct agtcaaacat tgtctgacca
    33421 tgaatatcaa atgcttcgtg atgcttctct ttcgattatt cgagcactta agatcgaggg
    33481 aggctgtaat gtccaactcg ctcttgatcc acatagtttt aattattatg tgattgaagt
    33541 caatccgcgt gtttctcgtt catctgcgtt agcaagtaag gcgactggct atccgatcgc
    33601 taagctagct gctaaaatag cgcttggatt aacattagac gaaatgaaaa atcctgttac
    33661 aggaacgact tttgcagaat ttgaacctgc attagattat gttgtagcta aaattcctcg
    33721 ctggccgttt gataagtttg aaaacggaga acggctttta ggcacgcaga tgaaagcaac
    33781 tggtgaagtg atggctatcg gtcggaatat cgaagagtct ttattgaaag ctgtgcgttc
    33841 tttagaaatc ggtacacacc atatcgaact tcctgagttg gcagacatca cggacgatga
    33901 cctaatccaa aaagtggttc gagcacaaga tgatcgcctg ttttatgtag ctgaagctat
    33961 tagaagaggg tacaccatag aagaattagc ggatttgaca aaaatcgatt tattcttttt
    34021 agataaactg ttgcatatcg tagaaatcga acagcagttg aaacagcagc cgttggatga
    34081 caagatattg aaaacagcga aacaaaacgg ttttacagac aaaaaagtgg cggagctgtg
    34141 ggaaataaac gaagaagccg ttcgccagat gagaaaagaa aatgatttga ttcctgtata
    34201 taagatggtt gacacttgtg cagcggaatt cgaatcaaaa acaccttatt tttatagttc
    34261 ctatgaatat gaaaatgaaa gttcacgatc agaaaagccc tcaattctag tactgggatc
    34321 tggtcctatc cgtatcggac aaggggttga atttgactat gcgactgtgc attctgtcaa
    34381 agcaatccag caggcaggat acgaagcaat catcatgaac agcaatcctg aaacagtttc
    34441 gactgacttt tctatttcgg ataaattata ttttgagcca ttgaccttcg aagatgtgat
    34501 gaatgtgatc gagctagaac agccgatagg agtaatcgtt caatttggtg gtcagacagc
    34561 aatcaactta gccgaacctt tgactgctgc tggtgtaaaa atcttaggga caacgatcga
    34621 agacttggat cgtgcggaaa atcgtgattt attcgagcaa gcgctgaaag agttggatat
    34681 tcctcaacct ttaggggata ctgctacaag taaagaagaa gccgtggtta tcgcttcgag
    34741 aattggttat ccagttcttg ttcgtccaag ctatgtatta ggcggacgtg cgatggaaat
    34801 cgttgaaaac cagcgagatt tagaagatta catggaacat gctgtgaaag catctccaga
    34861 acatccagta ctagtggacc gttacttgct tggtggtgaa tgcgaagtgg atgcgatttg
    34921 tgacggtgaa acagtactca tcccagggat catggagcat attgagcgtg ctggtgtcca
    34981 ttcaggggat tcaatggctg tgtatccacc tcaaacatta tctgaatcga tcaagaaaac
    35041 gatcgaagat tacacgatcc gcttagcatt aggtttgaat tgtgtcggga tgatgaatat
    35101 ccagtttgtc atccatgaaa atgaggttta tgtgatcgaa gtcaatcctc gagcaagtcg
    35161 gactgttcca ttcttaagta aggtgacagg aattccaatg gcacaaattg cgacgaaagc
    35221 tattttggga gagaaactaa agacacttgg ttatgaaaac ggactttatc cagaaacaaa
    35281 agaagttcat gttaaagcac ctgtcttttc ttttacgaaa ttgcagaaag ttgatactta
    35341 cttagggcct gaaatgaaat cgactggaga agtaatggga tctgacagac aattagacaa
    35401 agctttgtac aaggcattcg aagcttctgg attacacttg cctgaatatg gagccgtatt
    35461 atttacgatt gcggacgaaa caaaagaaga agcactagaa ttagctaaac gttttacaga
    35521 aattggctat agtctgatag gaacgaaaca tacagctgct tatttcgaaa aaaatggtct
    35581 ggtcgttgaa acagtagcga aaatttctga ggaagcagca gaaaaaaatg tagtcgactt
    35641 aataagagat ggtaaaacac aagtggttgt caatacgatt gataaagatc ggggaaatgc
    35701 ttctaaagac ggtttcatca tccgaagaga agcagttgaa catggtattc cattgtttac
    35761 ttctttagac acagcagatg cgatcgttcg agtgatggaa tcccgtgctt tttcaactaa
    35821 ccctatttaa cggcgagcac atgttaagac aaaaagacga aaaaccgttg caggagggga
    35881 cagatgaaac aagaaatgat ggaaatcgtt cagcaaaaac agctggcacc aaagattttt
    35941 gaaatgacat tgaaaggtga tctagtaaaa gaaatgcagc aaccaggaca gttcttgcat
    36001 attctcgtgc cgcgtgcaga cttgctatta cgccgaccga ttagcttgaa tcatatcgac
    36061 cacgaaacaa atacttgccg tatcatttat cgggtggaag gtgaggggac tagagtcttc
    36121 tctactcttt ctgccggaga ccatttagat gtcatgggtc ctttaggcaa tggttttgat
    36181 ttgtccaatc ttcatacagg agacgaagta tttataatcg gtggaggaat cggtgtccct
    36241 cctctatatg agttatcaaa acagttagtc gaaaaaggaa ttaagccaac gcatttttta
    36301 ggttttgcca cacatgaagt cgtttattat gaaaaagaat ttaatcgtct aggagaaaca
    36361 agaatcgcta ctgacgatgg aacatacggt atgcacggaa atgtcggtaa tttattgtta
    36421 ggaacgaaga cggaacctac agctgttttt gcatgtggtt caaacgggtt attgaagaca
    36481 gtggaacagc tgttttcttc gcatcaaaat gtccagttat cattagaatc ccgcatggca
    36541 tgtgggatag gggcttgcta tgcttgcgtt tgtcatacgc cggaagatga aacaggaaca
    36601 aaaagtgtga aagtttgtga cgaaggaccg atttttaaaa taggagaggt aattatatga
    36661 atgagaatcc tttagcggtc aaacttcctg gcctagaatt aaaaaatcct atcatgccgg
    36721 caagcggctg tttcggtttt ggcaaagaat acggaaaata ctatgatttg aatcagcttg
    36781 gatcgatcat ggtcaaggca acgactccaa atgcccgctt tggcaatccc acgccaagag
    36841 tggcggaaac accatctggc atgttgaatg caattggttt gcaaaatcct ggtctagacg
    36901 ttgttatgca taaacttctt ccagaattag aggagtacaa tgagttaccc atcatcgcaa
    36961 atgttgctgg tgcttgtgaa gaagactatg tagaagtttg tagcaaaatt ggcgaagcac
    37021 caaatgtgaa agcaatcgag ctgaacattt cttgcccaaa tgtcaaacat ggcggaattg
    37081 cttttggaac agattctgat gttgcttttc aattaacaca agcagtaaag aaagtttctt
    37141 ctgttcctgt ctatgtcaaa ttgtccccaa atgtaacaga catcgttcca atcgctcagg
    37201 caatcgaagc tggaggggca gatgggttta cgatgatcaa tactcttctt ggtatgcgga
    37261 ttgaccttaa gacaagaaaa ccgattttag ccaatcaaac aggtggactc tccggaccag
    37321 caatcaagcc tgtcgctatt cgtttgattc accaagtagc aagtatatcc agccttccaa
    37381 tcattgggat gggcggcgtg cagacagtgg atgatgttct tgagatgttt atggctggag
    37441 caagtgctgt agctataggg accgcgaatt tcaccgatcc gtatatctgt cctaaactaa
    37501 ttaaagagct ccctgtgcga atgagcgaac taggcatcga atcgctggaa agattaatca
    37561 aagaagtgag agaggaacgt gaacgatgaa tcaactagac caacgaccaa ttattgcatt
    37621 agatttttca actaggcaag aggtagaaga ctttcttcga tttttcccga aagaagaaaa
    37681 attgtttgtg aaaatcggaa tggaattgtt ctaccaagaa ggacctgaaa tcgtacgtta
    37741 tctaaaagag gctggtcata atgtattttt agatctgaaa cttcacgata ttccgaatac
    37801 ggtcgaaaaa gcaatgcggg gattggcaaa gcttggtgtt gatctcacct gtgttcatgc
    37861 cgctggcggt attcgtatga tggaggcggc aatgcgagga ttagaagagg ggactccaga
    37921 aggtggaaaa cgtccgctac ttctagctat cactcagctt acttctacaa gtgaagaaga
    37981 aatgcatgcc gatcaactaa tcgaagtttc tttagaaaaa agtgtgatcc attatgctag
    38041 ctgtgccaaa aaagcaggat tagatggtgt ggtttcttct gcttgggaag tagaagcgat
    38101 caaagaaatg gcaggagacg aatttgtttg cttaactccg ggtataagac cagaaggaac
    38161 agtcgcagga gatcagacaa gagtagtcac accttctcag gcaaaaaaga tcggctcaac
    38221 ttttatcgtt ataggtcgac caatcactca gtcaactaat ccttataaag catatcaaac
    38281 gattcaaaca gaatggagct aacctaaaat gaatgttgaa caaagtatcg caaaagactt
    38341 attagaaatt gaagcagtat ttttaaatcc aagtaatcct tttacatggg caagtggaat
    38401 caaaagccca atttactgtg ataaccgaat cacgatgagc tatccaaaag tccgaaaaga
    38461 gatcgcaaaa ggattagcaa gcaaaatcaa agaagcttac cctgaagtac aagtgattgc
    38521 tggaactgca acagcaggaa tccctcatgc cgcatgggta gcggaaatac tggatttgcc
    38581 aatggtctat attcgaagta aggcgaaaga tcatgggaaa ggtaaccaaa tcgaaggacg
    38641 tatatttgaa ggtcagaaga tggttgtgat cgaggatttg atttctacag gcggaagtgt
    38701 cttggaagca gctgaagcag caaaaagaga aggtgcggat atccttggtg ttgcggctat
    38761 ctttacgtat gagttgccaa aaggaaaagc aaatttcgaa aaagccgaaa tccctcttat
    38821 gacgttgacc aattattctg tattgattga agcagcttta gaagatcgtt atatcgatga
    38881 gcaagaatta actctgctaa aagaatggaa aaaagatcct gaaaattggc agattgaagc
    38941 gcgcttgatg aagatagaga aatccgattt gtgtctccag aatcatcaat tggaatagca
    39001 aaaatccaaa aactgattgc gagaagcatc ttttcatcag gtatgaaaag aaacttctat
    39061 cgtgatcagt ttttgatttt tttaaaattc atttttctaa gactggttag cagaaagaag
    39121 agaataatct cttctctttt ttctattata tatagatttt ctctattttt tggtaaggga
    39181 gctgtttcaa aaatggtaaa gagcggaaat gttgcgaata tcagttattt aatgatatga
    39241 tattcggaaa aagaaatctc aaagggaggt aacttttatg gggttgaatg aatttatttt
    39301 aggattgaac aatttagaga cgatcacaag ggcacctgga ttttttaaat atacagagca
    39361 tacagttgca gcacattcgt atcgtgtagc atctattgcg caagttctag gagacattga
    39421 agaagcgaat ggacaaccga tcgattggaa actgctctat gaaaaatcat tgaatcatga
    39481 ctacacagag cgttttattg gtgatatcaa aacgccggtg aaatacgcaa gcaaagagtt
    39541 aagagggatg ctgcaaacag ttgaagaaaa gatgacggac gaattcatca accaagagat
    39601 tccagctgag ttccaagaag tttatcgccg tcggttgttc gaaggaaaag atgaaaccgt
    39661 agaaggagaa ctattagcca tttcagataa ggttgacctt ctttatgaat cttttgatga
    39721 aatcatcaaa aataacccgg aaaaagttta taaggagatg ttcattgagg cagtgactac
    39781 gattaaaagc tttgaacatc ggccttctgt tcagtatttc tttaaagaga tatttccgga
    39841 actattgaac caggaatttt atggaaaaga ggcgttttta gaaaccgtct caacgattgt
    39901 aaagaaagaa gaaaaataaa aatgatatga gatgcagggg gaagtatagt tgaaaaagaa
    39961 tatggatgtc gaatggggct attctggaga caaaggtccg gaaaattggg cgagtttatg
    40021 tgattggttt gcgagaggtg cggaatttcc attgcagtcg cctattcatt tgaccaaggg
    40081 ggaagaaacc aaaatagaag gagacacgct ttcctttcat tatcaaaagc aacggtttac
    40141 tgacaaggaa ttcaaaaaca cgattcattt agtacctttt gaccaactca gttatgtaga
    40201 gttcaagaaa gaacgctatt atttgacaga tattcatttc cacttgcctt ctgaacatat
    40261 catcaacgga aaacaggaag atattgaatt tcattttgtt catatgaaca gcaaaaaaga
    40321 gaatctagtt gtgggtgtca tgtaccagct aatggtacag gaaggttggc tgtataatca
    40381 agaaaatggg gaaagttgga acttagaaaa acatgagcat tgggtaaatc cggatgtgtt
    40441 ctttccggag caaaaaagcc atttccatta tattggctca ttgacaactc cgcctaccag
    40501 cgggccaatc caatggtttg taatggatca tgtaggaaaa ctgaaccaag agtttcttct
    40561 ccccttcgat ggtcagtatg ctcgaccgaa caaccgacca atccagccct tgaatggaag
    40621 agaaatttat tatttcgagg agtttgagca atgaatattc agcagatgaa atacgtggtc
    40681 gctgtagcaa acaatggtag tttcagagaa gcggcaaaga aaatgtttgt ctcccagcca
    40741 agtctttcag ctggtatcaa agaattagaa accgagttag ggatcacgct gtttactcga
    40801 acgaatcgag gggcatattt aacagaagaa gggcaagact tcttaaatcg tgccgagaaa
    40861 atattggtcc agcttgatac attagaaact cactatctaa cgagtgaaaa gagagacaat
    40921 ttttcgatcg ctgcccagca ttatgatttc ttaggtcctt tgaccgcaca gctactcgag
    40981 gaattcaaag acgagatcaa acaatttcgg atcatggaga cgacaacagc gagagtgatc
    41041 gaagaagtca aagagcagca cagtgagatc ggcattattt atatgaatga gcataatcaa
    41101 gctggtatca gaagatattt acaacaagga gagctaagtg gccgcagcat tggaaaattc
    41161 aaaacgcata tttttttaag gcaaggacat ccattagctg aacacgcaga gatcaccaaa
    41221 gaagaattgt tatcttatcc tcaagttcgt tttacacagg atggaaataa tttcccgtat
    41281 ttttatgaag accttatcga gatccctgat caagaaacag tgatttatac gagtgatcga
    41341 ggtactttga tgaatatcgt tcttgaaact gatgcttacg cttcaggatc tggtattgtg
    41401 attggaggga ttcgcgagca cttgcggtta atccctttgg cagaaggcga actaaatgaa
    41461 ttttatatca tttattcggc gaaacgtccg ctcagtgaaa tcgctcaaag atttatcaaa
    41521 gagcttactc ggttactgga ccagcagagc taagaaaagc agatgagaac gctgtttatt
    41581 gaacaccgtt gatcaaaaaa gaggggttgt gacagaattt tgtcacaact cctctttttg
    41641 tagcaaacaa tttataacta attgctgttg tgggaaagta cgcaaatatg attctagaaa
    41701 cactcattga ttacctgttt tgtttcatag ctgtaatttt ttcttcttct ggtgtcacga
    41761 taatcgtctt ttggttttca taaataacat aaccaggctt ggcgccattt ggttttcgga
    41821 tatgctttac ctgtacaaga tcaaccggca cttgggcaga gtatcggtat tttgaaaaat
    41881 aggcagctaa ctcggcagct tctgtaattg tttcatctga gggcttgtca cttttaatga
    41941 tgacgtgcga accgggtata ttttttgcat gaagccaata gtcggttttt ttagctgtct
    42001 tcattgtcag ctggtcattt tgaagattgt ttttaccaac aagaatgagc gttccgtcag
    42061 aagaaaagta ttgatcaggc tgagatggtt tcttacgttt ttgttttttt aagttcttct
    42121 ttttcaggta tccttctgaa gtcaattctt ctttaattgc ttcgatatcc attgggccgg
    42181 caatttccag ttgagataag acagattcaa gatattggat ttcatctttg gcttcttgga
    42241 tttgttcgcc aatcagctta acagcgttct tcagcttttg gtatcgatgg aaatatttct
    42301 gtgcattttg atttggtgtc aatgccggat ctagtgctat tttgattggc cgatcttctt
    42361 cataataatt aggtaaaaca acttcttttg cgccgcgtgg tacttgtgtc agaaaagtag
    42421 taagtagttc gccatttcta cggtaattct cagcgttttc ggattctttg agtgtttgtt
    42481 cacgtttttt caatttattt ttatttcgtt ttagttcatt ttcgattttt cgaatcagtt
    42541 ctccgccttg ttgttttgcc cgatctttct ctgctttttc gtgataatag ctatctaata
    42601 attgactgag tgttggataa gctgttacag cagatgcctg ttcggacaat acctgataag
    42661 caatcggtgt gaaaaaatct ttattttccg tttcgtaaaa agtaggaata ggctgatggc
    42721 tgatggctga gaaaaattcg tcccatacaa ccatcttttc attaggacgt tccagtaatc
    42781 ttttggtcag ctcttgagcg atatcgaaac cgattccctg aaactgacgt tggattgctt
    42841 ttggatctag ttcagtttgg gaaagccgct gaaagatttt ttctttctcg gagctaaatg
    42901 ggttcaagac ctcttgtttt ggcggagcaa catattctac accaggaagc aaagaacgat
    42961 aagtgttttg agagcttcca atgtgtttga tcgcatccag tatttttcca gtttctcgat
    43021 taactaagac gatcgtacta tgacgtccca ttaattcaac gatcaatatg atattttgta
    43081 gatcacccaa ctcgtttcgt ttagcaaagt ggaaatggat cactcgatca ttttcgattt
    43141 gttcgatgtc ttctaaaata gcgccatcca aatattttcg aagcatcatc acgaagttag
    43201 gcggattatc aggattctga taatcgattt gggtaatctg tacacgagca taacttgggt
    43261 gcgcagataa taataatcga tggtttttgc cgcgtgaacg aatcaccagc acgatttcat
    43321 tttcataagg ctgatggatt ttcgagatcc ggccagatag taacgtttca cgcaattcgt
    43381 tgaccatcgc gtgggtaaat acaccatcaa aggacatggt tcattcctca tttcttttca
    43441 taagtatatt cattataacg aaaaaaagaa gaaaaaccaa tttggccgaa tagtaaagca
    43501 aaagtctaga aaaatcttgg actaattaca taaaaaacag tgtttctatc gttgaaatcc
    43561 aaatttttag gt
//
LOCUS       DAJSBC010000018        43029 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSBC010000018 DAJSBC010000000
VERSION     DAJSBC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.018"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            591..1280
                     /locus_tag="ONT65_000999"
     CDS             591..1280
                     /locus_tag="ONT65_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2328648.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ONT65_001000"
     CDS             1267..2475
                     /locus_tag="ONT65_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2328649.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ONT65_001001"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ONT65_001001"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR2328650.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT65_001002"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT65_001002"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR2328651.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ONT65_001003"
     CDS             complement(6578..7273)
                     /locus_tag="ONT65_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR2328652.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT65_001004"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT65_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR2328653.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ONT65_001005"
     CDS             complement(8472..8663)
                     /locus_tag="ONT65_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR2328654.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ONT65_001006"
     CDS             complement(8684..9574)
                     /locus_tag="ONT65_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR2328655.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ONT65_001007"
     CDS             complement(9561..10328)
                     /locus_tag="ONT65_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2328656.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT65_001008"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT65_001008"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR2328657.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT65_001009"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT65_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR2328658.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT65_001010"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT65_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR2328659.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ONT65_001011"
     CDS             14899..15282
                     /locus_tag="ONT65_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328660.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ONT65_001012"
     CDS             complement(15450..16208)
                     /locus_tag="ONT65_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR2328661.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ONT65_001013"
     CDS             complement(16226..17044)
                     /locus_tag="ONT65_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2328662.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT65_001014"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT65_001014"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR2328663.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT65_001015"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT65_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR2328664.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT65_001016"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT65_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR2328665.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT65_001017"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT65_001017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR2328666.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT65_001018"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT65_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR2328667.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ONT65_001019"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ONT65_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR2328668.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT65_001020"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT65_001020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR2328669.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT65_001021"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT65_001021"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR2328670.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="ONT65_001022"
     CDS             27086..27385
                     /locus_tag="ONT65_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR2328671.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ONT65_001023"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ONT65_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2328672.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="ONT65_001024"
     CDS             27973..28209
                     /locus_tag="ONT65_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR2328673.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ONT65_001025"
     CDS             28352..30325
                     /locus_tag="ONT65_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR2328674.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ONT65_001026"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ONT65_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR2328675.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT65_001027"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT65_001027"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR2328676.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ONT65_001028"
     CDS             33204..34496
                     /locus_tag="ONT65_001028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR2328677.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="ONT65_001029"
     CDS             35232..36095
                     /locus_tag="ONT65_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2328678.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="ONT65_001030"
     CDS             complement(36224..37846)
                     /locus_tag="ONT65_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328679.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="ONT65_001031"
     CDS             38095..38616
                     /locus_tag="ONT65_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2328680.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT65_001032"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT65_001032"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR2328681.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="ONT65_001033"
     CDS             40697..41515
                     /locus_tag="ONT65_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR2328682.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="ONT65_001034"
     CDS             complement(41618..41815)
                     /locus_tag="ONT65_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328683.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="ONT65_001035"
     CDS             41984..42562
                     /locus_tag="ONT65_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2328684.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42657..42794
                     /locus_tag="ONT65_001036"
     CDS             42657..42794
                     /locus_tag="ONT65_001036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328685.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT        13077 a         8244 c         8498 g        13210 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaata
//
LOCUS       DAJSBC010000019        41106 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSBC010000019 DAJSBC010000000
VERSION     DAJSBC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.019"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            128..931
                     /locus_tag="ONT65_001037"
     CDS             128..931
                     /locus_tag="ONT65_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2328686.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            918..1244
                     /locus_tag="ONT65_001038"
     CDS             918..1244
                     /locus_tag="ONT65_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328687.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            1593..2633
                     /locus_tag="ONT65_001039"
     CDS             1593..2633
                     /locus_tag="ONT65_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2328688.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            3469..3801
                     /locus_tag="ONT65_001040"
     CDS             3469..3801
                     /locus_tag="ONT65_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2328689.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4392..4721
                     /locus_tag="ONT65_001041"
     CDS             4392..4721
                     /locus_tag="ONT65_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328690.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            4708..5049
                     /locus_tag="ONT65_001042"
     CDS             4708..5049
                     /locus_tag="ONT65_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2328691.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5163..5402
                     /locus_tag="ONT65_001043"
     CDS             5163..5402
                     /locus_tag="ONT65_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328692.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5469..5708
                     /locus_tag="ONT65_001044"
     CDS             5469..5708
                     /locus_tag="ONT65_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328693.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            5794..6465
                     /locus_tag="ONT65_001045"
     CDS             5794..6465
                     /locus_tag="ONT65_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2328694.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            6518..8494
                     /locus_tag="ONT65_001046"
     CDS             6518..8494
                     /locus_tag="ONT65_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2328695.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            8507..9163
                     /locus_tag="ONT65_001047"
     CDS             8507..9163
                     /locus_tag="ONT65_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2328696.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            9259..10086
                     /locus_tag="ONT65_001048"
     CDS             9259..10086
                     /locus_tag="ONT65_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328697.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            10096..10344
                     /locus_tag="ONT65_001049"
     CDS             10096..10344
                     /locus_tag="ONT65_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328698.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            10387..12486
                     /locus_tag="ONT65_001050"
     CDS             10387..12486
                     /locus_tag="ONT65_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2328699.1"
                     /translation="MENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(12524..14581)
                     /locus_tag="ONT65_001051"
     CDS             complement(12524..14581)
                     /locus_tag="ONT65_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR2328700.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(14695..14907)
                     /locus_tag="ONT65_001052"
     CDS             complement(14695..14907)
                     /locus_tag="ONT65_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328701.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            15094..15561
                     /locus_tag="ONT65_001053"
     CDS             15094..15561
                     /locus_tag="ONT65_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328702.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            15558..15800
                     /locus_tag="ONT65_001054"
     CDS             15558..15800
                     /locus_tag="ONT65_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR2328703.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            15818..16120
                     /locus_tag="ONT65_001055"
     CDS             15818..16120
                     /locus_tag="ONT65_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328704.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            16191..18245
                     /locus_tag="ONT65_001056"
     CDS             16191..18245
                     /locus_tag="ONT65_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328705.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            18245..20077
                     /locus_tag="ONT65_001057"
     CDS             18245..20077
                     /locus_tag="ONT65_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR2328706.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            20790..22688
                     /gene="ltrA"
                     /locus_tag="ONT65_001058"
     CDS             20790..22688
                     /gene="ltrA"
                     /locus_tag="ONT65_001058"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2328707.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            22805..23605
                     /locus_tag="ONT65_001059"
                     /pseudo
     CDS             22805..23605
                     /locus_tag="ONT65_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            23602..26133
                     /locus_tag="ONT65_001060"
     CDS             23602..26133
                     /locus_tag="ONT65_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328708.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            26183..26515
                     /locus_tag="ONT65_001061"
     CDS             26183..26515
                     /locus_tag="ONT65_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328709.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            26516..27109
                     /locus_tag="ONT65_001062"
     CDS             26516..27109
                     /locus_tag="ONT65_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328710.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            27272..28966
                     /locus_tag="ONT65_001063"
     CDS             27272..28966
                     /locus_tag="ONT65_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR2328711.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            29654..31531
                     /gene="ltrA"
                     /locus_tag="ONT65_001064"
     CDS             29654..31531
                     /gene="ltrA"
                     /locus_tag="ONT65_001064"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2328712.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            31662..32039
                     /locus_tag="ONT65_001065"
                     /pseudo
     CDS             31662..32039
                     /locus_tag="ONT65_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            32039..33244
                     /locus_tag="ONT65_001066"
     CDS             32039..33244
                     /locus_tag="ONT65_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2328713.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            33260..33904
                     /locus_tag="ONT65_001067"
     CDS             33260..33904
                     /locus_tag="ONT65_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328714.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            33915..34721
                     /locus_tag="ONT65_001068"
     CDS             33915..34721
                     /locus_tag="ONT65_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328715.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            34743..34973
                     /locus_tag="ONT65_001069"
     CDS             34743..34973
                     /locus_tag="ONT65_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328716.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            35519..35794
                     /locus_tag="ONT65_001070"
     CDS             35519..35794
                     /locus_tag="ONT65_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328717.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            35836..36258
                     /locus_tag="ONT65_001071"
     CDS             35836..36258
                     /locus_tag="ONT65_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328718.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            36277..37869
                     /locus_tag="ONT65_001072"
     CDS             36277..37869
                     /locus_tag="ONT65_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328719.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            37881..38216
                     /locus_tag="ONT65_001073"
     CDS             37881..38216
                     /locus_tag="ONT65_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328720.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            38363..38557
                     /locus_tag="ONT65_001074"
     CDS             38363..38557
                     /locus_tag="ONT65_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328721.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            38589..40961
                     /locus_tag="ONT65_001075"
     CDS             38589..40961
                     /locus_tag="ONT65_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328722.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT        15245 a         6354 c         8307 g        11200 t
ORIGIN      
        1 ataatcttta tatccgaacc gatatatatc tttaaaacaa aataatcaca caatattaca
       61 atataatgca tatacgatat tgcaatatca caatattatg atataattat attataatat
      121 aagatgaatg ggggatattt taatggcaag aatattgtca gtagccaatc aaaaaggtgg
      181 agtttctaaa actacgacta gcgttaattt agcagcatgt ttgaaatttg tttacggtaa
      241 aaatgtactt ttagtggaca ttgatcctca gggaaatgct acagataatg taggtttcga
      301 tatagatgga acaaaccaac ctacaattta tgaagtatta aaaggggagg ctgacattac
      361 agatgctatt ctggactata aaggaattga tgttttgcca gccgacatag ccctttcttc
      421 tgcagagagg gaatttactc aagtaggaag tgaacacagg cttaaaagag tacttcaacc
      481 aatcaaggac aactatgatt atattataat tgattgtcca ccaagtttag gaatgcttac
      541 agtcaacgca tttactgttt ctgatgaaat tatcattcca gttgaagctg cgtatttctc
      601 attaaaagga ctaataaagt taagtgaaac tattgaaacg gtaaaagaat atacgaacga
      661 caagcttgaa gtacgaggag tcctatttac taagtataat aagcgttata acatttctaa
      721 agaaatgacg actgcagcaa ccgaaatttc tagagtcatt ggtgcaaaaa ttttcaaaac
      781 ttatattcgt agaacaataa ccgttgacga agcgcaagct gctggatcag atctaatcaa
      841 ttttaataaa ccctcaacag ctgaagatga ctataaagca tttacagaag aatatttaaa
      901 agaagttggt gattttgatg gcagaagata atcgcagtca agctgcagta agtagactac
      961 taggaaataa aaataaaaag aactacaaac caaaagaagt agaagaaatc gaattaacca
     1021 agaaaaatat aagtaaagtc ataaaagatc aaggaagtag tgtaagaaca gcagatagca
     1081 ttcgtataaa tccaattcta atgtcagcac tcaaatattg gacgactatt gctgaatcag
     1141 acaaaagtaa accagacgtt atagaagatg cgctattaaa atccatacct gaagaatact
     1201 tgattgaagg atataaacta gcaaagaaac aaaataaaat ttaatttaca ttcaatatca
     1261 cgatatcata atatcgtgat atttttttta taaaaatcag tagtatcaaa gctgataagt
     1321 cgcttatctt catattgtaa tattacgata tcgtgatata atgttattgt aatatcgtga
     1381 tatagttacc atgagcttaa accctgaaat agagcggttt gctttaattt attttttaag
     1441 gtaaactaaa aaaagataaa aaaagactac tgctttaaca aagtttggcg acttatcagc
     1501 agtagaaaaa tatatagtgg atagatatat ttgtttaatt cattgtaaca tagttttgaa
     1561 caaaacgcta tcctacagaa agaggattgg ctatgaatga ttttaattat tacaaatcaa
     1621 aagaaattta tcgtgaaaaa tattatcaaa tgcctaaggt attttttact aacgaaaaat
     1681 acatggatct ttcaaatgat gcaaaaattg cttatatgct acttaaagat cgatttgatt
     1741 attcagtcaa aaataactgg gtagattctg atgataatat attcttcata ttcacagttg
     1801 aagaattaat gaaattgtta caatgccgcg aaggtaaagt atctaagatc aaaaaagaat
     1861 tagaaaaagc aggtcttctg aaacagaaga gaggaagagt aaataaacgt gatggtaaga
     1921 ttgaatctat gcctaattta ttgtacttag gcaagccaga agttacgaat caagacgtct
     1981 ttaaaatcat ggaagaggaa gacaatactg attccaccgt aattgcgaaa atcgcaaata
     2041 cggcaaaacc gcgaaaaatc aatgattcta ccgtaattgc gaaaatcgca aatacggcaa
     2101 aatccagtca tatcaaggac tcaaccgtaa ttgcgaaaat cgcagacaat ctattctatt
     2161 ctaattctat agacactaat agacacataa tagacactga aactgaccag ttacaaaatc
     2221 aagttttgct agataacttc gtcgacatca tgcaagagga cagtataaat acatttgtac
     2281 cagaaaatgt attaaacttg ataaaaacat tttcttcaag ttacagtgaa gctcaacaaa
     2341 ccgtccaaac tattcataat gcaaagaaaa aagctgaaga attagagcaa actattgtcg
     2401 tttatgaaga actggaatct tatggaattg atgctgacaa aggattatat atgactttac
     2461 tgaaagctta tcaaaaacaa aaaactgaaa aagtaaacaa tttgcagaat ctaattttcg
     2521 tttatgtaaa aaattggttt gtcgaaaaag ctattgcggc aaagcttgca aacgaacaaa
     2581 gagaaacatt taacgaatta ccaacagtta gtacggaaaa ttggttagag tagttgataa
     2641 aaagcaatta ctataaaaat gatcaatttt aaattagaac ttttttatga ttttaatttt
     2701 attacaaata ttacaaataa atagttagta ttccggagtt tttagattaa aagtttatga
     2761 aaacaatcac aatcgtaggt tttaaattca taatcgttag atattttagt tatttttcac
     2821 tcaaaaaaat agggaactag tgtttgcata tgtgcttttt tgagttataa taattcatga
     2881 agagaggtaa ttcacttatt tccccaagct gaaaagcctg atattacttc ttttcaggca
     2941 gtgctgctaa tattcaatta cacttttttg tatattttta gttgttttaa atttaattgt
     3001 ctgctatgct tgtgatgaat tgattttcgt tctgcacttt tggacgataa atttggacaa
     3061 ttgaaaatac aaaaaaaaga aaggccctgt gagactcgga aggctggtaa ccttcgtcga
     3121 cgtccacagg tggcattaag atcactccct taatgcgttt acctttcagt gttacggtat
     3181 tacccgtaac ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg
     3241 ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa
     3301 tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga
     3361 tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa
     3421 caagaaaaac ttgttaaaaa tatttaaagt aagtgggtgg attattttat gtcatataca
     3481 ggaattttga gtctcgaaga tatttgtcac tacggtaaac ggtgtaccgc tacagaaaag
     3541 atcacgaaga aactatcaac tggtcaaagt aaaaccgttg tgcagtgtaa gaaatatata
     3601 atccagaaag acaaagtctc agaagaaatg atttattaca ttgggaaaca aaaacaaata
     3661 attttaaaag atcctatccc tttaaaagag ctatatccaa caatcaaaca tgtttatgac
     3721 caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg
     3781 atggggcgtt taatcagctg aattgatcat tctcataact tgtattttat cgtttttcgc
     3841 ttgcttgaaa atattgtcga gacttctaaa ggaacttaga atcactagaa aaataaggag
     3901 tagacttgat ctatcaacaa ttcctttcgg ggggtgacgg ggtaggtttt tacgattggt
     3961 aacgtaaaaa attggtcaat cgagtaaatg cccttgtttg gatagtggtg aaatgaattt
     4021 gtgtaaataa tatgcacaga gtaaacaaga gggaatttag aagttagaca agttagaata
     4081 gcgcctagat taatcatgag cagctactaa gggatctagg ctttgacgca aactcatgga
     4141 ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca
     4201 gtacatgtag atacttgcga agacgttgat ctttggatca atcgagcatt taccgttttc
     4261 tactagggtt tctcgtttcg gacgccataa acgagtcgat ttattttata tatttttgag
     4321 tgaacgaaaa acgataaaaa tattcgttat gaagtaaaac taatttagta gatagcaaag
     4381 gtggtagagg aatgattcaa aaatttgcga aaatgagtaa aactgcatgg attgaaaagc
     4441 ttgaaaatga cttgagtgtc atgcgattag ctccaaagaa attcaaacga gagattgcag
     4501 aaaaagaagc aattttgtcg gtattgaatg cggtagacga taaatgtcca gtaaaaatta
     4561 gtttttcaga tatagcaaca attatcgaag gtatttatta tggtgaagtt gatgaagaga
     4621 tatcttctac aaaagattta ttagattttt tggataaaaa caaagatacc tttatagtac
     4681 taactgatta tgaggaggtt acaacaaatg aaaaattatg atccgaatag ccgatgggga
     4741 acacatacga ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga
     4801 agaggcggaa attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat
     4861 caaaaattga ctgataatcc aattggattc ggcttgctac ctgaagatga tcaaggtaat
     4921 gaatggttta aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact
     4981 tggtcatatt tgagtgatta tattgtcagc gttgaaatca ttgaatttgt agcggataaa
     5041 gaagaataaa aactggagga ataaatttta cagataaagg cttagaagga gtcacgacga
     5101 ttaataaaaa ggtagtatta ccttaggcat tgagtaagga atttatttag gaggaaaatt
     5161 agatggcgcg accagtttta gaggcgatgg atatggatac tttgacaaaa ggaaggtatg
     5221 tacagctgtt agctcgtggt tattcaccta aagaagtatt caaatccctg tctaaaggaa
     5281 cagatatgga aaaagaaaga ttacgaaaag aattcgatta ttggagaacc cataatggaa
     5341 tcaaggattt gaaaccagca cggccagtaa ttaaacgaaa gaaaaagata acccaagact
     5401 gaagtgtaga gcgtatgtga tcataggctt ttttattttt gcaaaaaaca aggaaggtag
     5461 gaaaactcat gaattatgaa gaaatcgaaa acagaaaaaa agtgtcgaaa gaaatggaag
     5521 aaaagttgct aaaaacgatg aaacaaaaac accttaaaag attatctgtc atgcaatata
     5581 tcaatgatat gcagataaca ggaaaagaga aagcttgttt gttaggttca atgaaaaact
     5641 ttgaacagtt gagacgaact tatgtgaaaa aaagttctaa ttgtcaacta ttgttagaag
     5701 tctcatgaca ttcattaata gaattcattt ttaaaacttt ttctatggaa atgagaaaaa
     5761 gattttttgt tttgtctgaa agggggtagc tggatgagaa aatggttatt tggtttttta
     5821 ggagtcgctt taatcgtctt ttgcagtgtg tttggctatg ttagctacca aaaacatgaa
     5881 ggaaaggtgt ttaagcaaaa tatagaaaaa aagatgcccg tagatcaaat caatgaacat
     5941 gcaaagagtt ataaggagga tgcaacgaac gtcaataatg atatgtcttt ggggcaaatg
     6001 ttgtccatac aaaaagaagc aatcgaaatg ggtgtcaata aacaagcctt tgcacaaatt
     6061 cagatacctg cacttggttt agcattgcca atttttaaag gggcaaatca atacactttg
     6121 agtcttggag cggctactta tttttatgaa gatgccgaaa tgggcaaagg aaactatgtc
     6181 ctagcaggtc ataacatgga aatgccaggt gttttatttt ctgatattca aaagttgtca
     6241 ttaggggagg tgatggactt agttagtaac gatggcgtct atcgttacaa ggtaacgcgt
     6301 aaatttatcg tacctgagta ctttaaatta atcgatggcg ttcctgaaga gaactctttc
     6361 ttgtctttac caaagaaagg agagaaaccc ttattaacgt tgtttacttg tgtttatacg
     6421 tcacaaggaa aagaaagata cgtggtacag ggagagctac aataacaagg ttttaatcaa
     6481 cgcgagtcgt tgaaatatga aatgaggaga gtaaaaaatg gcaataaatc ggtatgtaaa
     6541 aaaagctgga atgcttgttg gaatgttagg aatcattgca agctgctttc aggggtatca
     6601 agtacacgca gaagatgtca cgcaaaaaac accaccagag aaggtcaata tcacggttca
     6661 taaattaatg tacgatcaag gaacgcaatt gaatgttgat attgacggga tcaaaaacga
     6721 tggttatacg cacgatcaat atcctacagg tgtaacgaaa tacaacaagg cagattatgg
     6781 tgatgttgag tttacgttag gaaatattac ggatcaagta ttaccgagag aagattctga
     6841 tctaaccaac gcgaaagtgg acgagattgt acaagatgta gaagacaagg gatctaacag
     6901 tgagtacgtc aagaatgcaa ccaacaaaat tacttcagct gtagatgaaa atggtgagat
     6961 cacttttact gatcaaccag cctatgtcaa ttccaaaggg aatgtatatg tggtttatga
     7021 aagtaaatcc gctgctggat tagtcacaca aaaagcaaaa ccaatggttg tgattgcgcc
     7081 aatgacagat aaaacaggat caggattttt aaaaaatatt catttgtatc ctaagaacat
     7141 tgtttctaag ttgagttttg aattaacgaa atttggtgac gatggcgcag cccaaagcaa
     7201 acaaaccctt ttaaaagggg caaaatttga gttatacaag ggcgaacctg gaaaaggaac
     7261 aaaattgggt gatcttgtat cggatgatca aggaaagctg actgcgactg atttgacttt
     7321 aggaaaatac tattttgtgg aagttccttc ggaagttgta gtgggatcag atcaagaacc
     7381 aacagcagat caatatttat taggcgcaga cgctcgcaac gatgcgcata ataaattaac
     7441 ctttgaaatt accaatgacg gcgtgacaag tgacttaaaa gcttcctacg tgaactataa
     7501 agcaccagtg atcgataaaa cagtgacaaa tggaacagga caagaacatt ctttccaaat
     7561 tggtgacgca gtgaattatc aaggaacgat tcatatccct acagatattg caggtggaac
     7621 agatgggatt acagtaaatg gtgtgaagag tgaaacttct ccttacagcg tatttaagtg
     7681 gggagataca gcaggacaag gactttctta tgtcgctgct aaagcaaata tcaaagtaac
     7741 aaataaagat ggatctgtgg tattaaaaga aaatacagat tataaaatcc aaaattcaga
     7801 aaatggcttt gtgatcgatt ttatcgtgaa taatggccaa gtaagtgata ctgtagcaag
     7861 tttacacgga caagatcttc aaatgactta taacatgtat gtaaatgaca gcgcagctgt
     7921 tgccaatcca ttaacaaata gcgtggattt cgtttataac aacaatccat ttaatcagga
     7981 agaacaccac gaaaaaacga aagctgacgt tgtgacttat ggtgcaaaat tcttaaaagt
     8041 cgatagcgga ctatttggaa caggaatcaa agcaacaccg cttgaaggcg cagaatttgc
     8101 tgctaaaaac gcagaaggaa aatattatgg tggattagtt gatacggata aagacggtgt
     8161 caaagaagca gtatgggtag acgatgtagc aaatgcagcg atcctaaaat cagataaaga
     8221 aggtcacttt gaaatcactg gtttaactga aggtgaatat tcactggaag aaacaaaagc
     8281 accaactggt taccaaaaat taacagaaga tattgcattt aaagtaaaca aaaactcatt
     8341 taaagaggaa aatcgaatca ctgttaaaaa taatcaaaaa gcagctattc cattaacagg
     8401 tagtaatggt ttccaaacgt atgtattgat ctcttgcttg ttgctaggag caggggcact
     8461 ttctgcagtt gtttacttta agaagaaagc ctagaaggat cagctaatga ttattcgtaa
     8521 gcccccaaaa agaagagtca tgcacaaact gatgatattt ttttggttgc tgtcggcagt
     8581 agcagtctat ttaattccag ctgtggaaac aaatgtagcg aaatttcaac aacagcaaag
     8641 aattttagcc attgaaaatc ttgaaaaaac agattcttct ttggatgttg cgaaaataga
     8701 gttaggcgat cctgttggtg ttcttacaat tccaagtatt cctttaaaac taccaattta
     8761 tgacggaact tccgacaaaa ttttagagaa tggtgtaggg attacggaag gaactggcga
     8821 tattacagga ggaaacggta aaaatccttt aatcgcgggg cacagtggac tttataaaga
     8881 taatcttttc gatgatttgc cttcagtaaa aaaagggcaa aaattcttta ttaaggtaaa
     8941 tgaagaacaa catgcctatc aaatttatcg aattgaagaa gttaaagcag aagaattaca
     9001 aaaaaattat ttagattatc tcatgcctga aaaagaccaa gataggatca cgttaatgac
     9061 atgcacgcca aaaggtgtta actcacaccg ctttttagtg tatggtgaac gagtgtcatt
     9121 cagtaaagaa gatttaaaac tacagaaaaa agcaaaaaaa taggaaatca atttcattgg
     9181 attggcgatt agcactgcta ttcgcttttt ttgcgttcct attattttta tttataaaaa
     9241 aataaggagg agatcggaat gaataagaga aaatggctgt ttgtttgtgt agcagctctc
     9301 tttgcaactg cagcttactc acagatatct tttgctgata gcagtactac tccaactacc
     9361 agtcagacaa cagatagttc aacaactgca acaagtgaca cggacagttc gaagaacact
     9421 gcagaagaaa cggcagattc aagtacgagt tctactgaat cagcggaatc aacgtcagaa
     9481 agttcttcag agacttcaga aaaactgcaa gaagtagaag atacgcaagt aatcaagcaa
     9541 aacattcata ttcaaaaaat tatttcttcg aatcgaatca acaatcaagg aaggcaagtg
     9601 actcagcaag aaggtgtcaa tggtgcaaag tttattgtct acgatatcac gaatatctta
     9661 gaagaaatga cacaagatga taatgagaaa aatcaatcgg tagaaataat tgagtcaaaa
     9721 ttgaaagatc gagcaaaaaa actttcatac gatcagttga aaaaagtgac tgaaggagaa
     9781 acccaaacaa ttgatgatca ggaaggcgtg attgattttt caattgaagt acctgcaaac
     9841 aaaaaacaag catactatat cgtcaatgaa tcaagccctg aaaacatttc aaatagcgaa
     9901 gatattgttt tattgacacc tgtttcagat gaaaagggtg aatttttgac ggatgtttgg
     9961 ttatatccta aatcaaagaa atcagagccg aaagaagaaa ttaaaaaagt tgtatcaact
    10021 ggtgtaaaga aaaacttttt tgataattgt tgggattttt tcactcattt attttttaac
    10081 gattaggatg aaatgatgaa aataataaaa atgttagttt ttaatctatc ttttttagcc
    10141 gcaacaatat tgtgttgcgg tttagttttt tctcaattat caatttaccc aagatatcaa
    10201 cgattgttcg ttcattttct aaatatccag catgcttcca tgccactaga agtgattaac
    10261 aagctcttaa tcaatccaat ttttatggct agtttgtttc ttcttgcagg attggtaacg
    10321 tgggtattag gaaaaaaaga atgagaagct tacaatcgtt taacaaaata atagatggag
    10381 ggcattatgg aaaataaaat tcattgcact acgggaagag tatttacttt agtgttgttt
    10441 ttgttccttc ttctttttgc aaggggaact gcagtccagg ccttagaaaa agttgatccg
    10501 agtcaaatca aagaaaataa taatctggta ctttatcatg atagtgattc attgagaaat
    10561 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
    10621 aaagtacttg cttggccaaa agtcaaagat ttgaaaagca cgtcaaaaat tgttgcccat
    10681 tatcaaaagg tgggcactta tctaggaaaa gaaattgatt gcgacgtaac tttttctaat
    10741 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tgggaaaaag
    10801 tattataatt atttgctctt tgataaaaac atttatcgag gctttttcat gcagtcaatc
    10861 aacgttatga atgttaaact agattttaag tatgcaggat caaaaacaaa agtagctttg
    10921 agtgaaccac agccagaaat taattcaaaa atgagatcga tgagagcagc tggtattgag
    10981 ttgaatgata catttctatc tatttacagt ttaaatggac agtatcagca acgtgtgtct
    11041 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
    11101 gggtacgaaa aaatggatcg cttaccttat tttgtcacaa cagatacagc atgtgcagaa
    11161 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
    11221 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
    11281 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
    11341 acgaatagta tgatgtttag ctttgatact gcattgcttt ggacaccaca accatcgaag
    11401 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
    11461 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
    11521 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
    11581 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
    11641 aatgggactt ggaaaaaagc tgatacagga ggaaaattaa ctgtatcagg taatacagtc
    11701 acttatactg cttctgacag ctttttagct aatgctgcaa attacggtag ggactacatt
    11761 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
    11821 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaataaagt agaaaactat
    11881 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
    11941 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
    12001 gtcacacaaa aatggcatac aaaaggtgta gacattgcgc tcgatcatta caagcaattt
    12061 agtattcaag atccagtgga agctcgtttg acgtataaag aaggttctgc gcgagtgata
    12121 gatatgagta caggaaaaga tattacttca gaaggtatac taacctatga tagccattca
    12181 agaacgctga aatgggaagc ttccgctgat ttcttaaata acaatctttt agatggacga
    12241 gaaattcaac tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
    12301 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
    12361 aaacctaact tacctcaggt cattgtgcca aaaactggat cgaatcattt agtgctaact
    12421 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
    12481 aaataaatat aaaaacaagg ctgattttca gccttgtttt cttttatttg aagattggat
    12541 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
    12601 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
    12661 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
    12721 gcccagtttg acttatgttt tctcccttag caccatagca gcctaagctt tccaataaat
    12781 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
    12841 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
    12901 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
    12961 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
    13021 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
    13081 gatcagtcat atttttccaa ggagtagaga agctatcccc ttcgtatact gtttttgatt
    13141 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
    13201 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
    13261 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
    13321 tttcaccgtg agtgacgttg attatctgtt ctttcaaaat atcaactcgc ccctcatttt
    13381 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
    13441 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
    13501 tatctttagc accttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
    13561 aagtcacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
    13621 tggtaaccat atattctttt ggcgtttcag gtttgtgttg caacccttta aatgcagcat
    13681 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
    13741 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
    13801 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
    13861 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
    13921 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
    13981 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
    14041 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
    14101 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
    14161 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
    14221 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
    14281 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
    14341 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
    14401 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
    14461 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
    14521 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
    14581 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggttaacc
    14641 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    14701 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    14761 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    14821 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    14881 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    14941 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    15001 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    15061 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    15121 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    15181 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    15241 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    15301 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    15361 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    15421 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    15481 gatgagaagc gaacgaaaga gaaattaatt catgatggat cacaatcaaa taaatacaaa
    15541 ttacaaaaga ctagcgagtg attgctagtc tttttatttg tcctgaagga ggaatcacag
    15601 tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa aatcttagaa cgccgcggtg
    15661 tatctgaaac acagtggaaa caagaagtcc ttgaaaaagc acaatttgac ctattacggg
    15721 gaaaagacaa ggacttagaa ggatatatct atcaacgtga acgtgaaaag cttgttttcg
    15781 atgaaattat taaacactaa aaggagcgaa caattttatg tttgaatcaa ttacaatgaa
    15841 ttttttaaca caaccgaaag tagtagctgc ggttgatatt tttagagcat tacaaactgc
    15901 cgcaactagt ttgaaaaata atctgatgat ttttgctgct gttgcggcag tggcttccat
    15961 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    16021 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    16081 aatgttctca atgtttggac aaaccccaca aggactataa aaaaacaagt agaaggagta
    16141 aaaagtacgt ggctactttt tactcctttt tttatggaag gagaatattc atgtttttta
    16201 cgatttcagg caatgcaaaa ttggataaag agttaataag aaaagcaacg aagaaagtgg
    16261 gaaagcgtac ttccttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac
    16321 agctacatgt gttaggacaa gtgttagaac aagagaatca atcttctgtt aaattgactc
    16381 tcacgttaca tgataaagaa ggaaaaatag aaaaggctgg cacagaatac gtttttggct
    16441 cactggatta tcaaatttca gaaggttttt atcctacatt gattgattta atccgcctag
    16501 atatatttaa agatacggat gaaaccatga gtgtggaaga gtcttatcga aacgaaaaaa
    16561 ttttacaagt tttagaagaa gagttagaaa gcgaccaaag aaaagtgaat caagtgagtg
    16621 aagtcgaagc aaacgaagaa gtaccatggg aacgcccaat ctttccaaag gatgaatcaa
    16681 aagaaaaaga gaaaggaatt attgacgaac aagctgacat tttgactcat aaatcaatat
    16741 ttgaggaaga gaacgaggta atgggtcaat tggaggtaga tccctttgaa gaaacaatgg
    16801 aggatgaaga gacggccgaa gtagaagttg atccagttga agctaatgaa gacccaacat
    16861 ggactgaagg ggattctcag gaatatattg aaccacttat cagtgaagaa gtagagaaaa
    16921 atgaaacaga gatacaaaag gcaacggtat ttgattatat tttggatcaa tatccgccag
    16981 agcatgaatg gatgaaagaa aaaaataaat tatttttaaa tgaactttat tcgaaatatc
    17041 aattacctca aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta
    17101 aaggcacact tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac
    17161 aagaagattt attagctact tttgaagaac gtcaacaaga aacagatcaa gaaattaatg
    17221 agtatgcgac ggaacagtcg tcgaattggg agaaagcaaa agaggaacta aataaagaag
    17281 aacagcgagt gattgaagaa gaagtacaaa aaattaaaga gcggtacgaa aagaaacgtg
    17341 acaatgcgta ttccttgtgt gtagaaaaga tcaacaattt tactcagacg aatcaagaag
    17401 cattatcgaa agaaaaagaa aacttattaa aagaacgtat aaaagaacga aaagaagaat
    17461 tttaccgagg gttgcgtact gataagctaa ggattagcaa agaattaaat gaacaactga
    17521 ataaactcta tcaaactacg actgaagttc tgatggaaaa acacgagcag atccaacaag
    17581 aattacaagc acaaattcca aaatggaaaa aagagtatga aattcaccaa caagaattga
    17641 aagaacaaga agaaaaacaa cggaaaaaag aaaaagagcg cgaacaatta gaactaaaaa
    17701 aacgtgaaca ggatcttgcg gaacaaaaaa tggcgataga aaaacaacgg ctagaacaag
    17761 aaaaggaaaa agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac
    17821 aagcacagtt aaattatctg acacatcctc aaccagtagc tgctgttcaa acgccagtga
    17881 acaaccaagc aaatttaact ccttcacccc agcgttcctt taacggatgg atggtgggat
    17941 gtattgcaag tttaagcttg cttctaggtg gaggaacagt atttgctttt aatcatatca
    18001 atcaatcttc acaggctgat tctgtagcca caacggcgtc aatcaaggaa gaagcccagg
    18061 aacaagcaag aaaagagtat gaacaacaat tagaagaatt attgaatcgt accaatcagt
    18121 caacaacaga gtcaagcatg gaaaatagca aagagagtga ttctagtaag gctaaagaag
    18181 gatcaagcga ttcaactgta caatcaaaca cttccgaaag ttcagaatca aatgaggtga
    18241 aataatgcgt gttagagaaa caacgaagcg aaaagacaaa cgattcaatt tagaaaagaa
    18301 taggtatctt tctcatttct atcgctatac tccctcagga gaagagctac cagaaaaaca
    18361 tgacaaacgc atttcgaaaa agatttttta tagtggactt gtatacctcc ctttagagta
    18421 tttagtaaca agagtaccag aagttttcaa ccgattggaa attgttaagg gaagcttaca
    18481 aggaataaat ctgtttcaat tacaggaaat gccacaggtc tttcattttg attcagctgg
    18541 tttttggaat ccatttactc ctacaactag tttgggtaca cgtttagcaa tttcgacagt
    18601 ttgtgtggtt ccagctgtac ttatgggatt gaaaatggac atgtggtttc gtcctctagc
    18661 agatggacaa aaaggggata atcgattaat gaccttaaaa gaaatccaac agatttatcc
    18721 gcaaatacct gaaacaaaaa ctacttttcc aggttatgga ggtattccaa tcacacatta
    18781 caaaaaatat tggtacattc aaacagatac tgttaatacg tgtattgttg gtacctctcg
    18841 atcagggaaa ggtcagattg aagtgttagc caccattgac aatttatctc gtgcagaaaa
    18901 acaaagttca atggtcgtta atgatccaaa aacagaatta tatgttggta gtaaagatga
    18961 actagaagct agagggtatg atgtatatgc ctttaatatc accgatccac tgcaatcaat
    19021 gagtgataat ccattggcac tcatcgtgaa atactggaaa cgaggagaca tagatactgc
    19081 aacacagtta accaatactt tcacaaatac gatttattat gatgccaacg cttcagataa
    19141 caaatacttt aacgaaaacg cgcagaaagc tgtcaatgcg cttatttttg cgttgttaga
    19201 acaagctgat caaacaggag attactccaa attgacaccg aataatctcg ttgagcttct
    19261 ttctgagatt ggcggtttta attattatcc agatccagac aatgaattca aacagttgaa
    19321 tgccttagat gaatttatga atcagttgcc acctggaaac attgcaaaaa aacaatatgg
    19381 ggcaacgaaa attggatctg ataaagcgaa gggaaatatt ttatcaacgg caatcacggg
    19441 attgaatcca tttactctaa cgaaaattgc aaagatgact tctcaatcaa cgattgatta
    19501 taaatcggtt gggtttccaa aatacttaga tttgaaatta catgaaagtc ttttaaatca
    19561 acgagtcgta gtagaatttt atcataaggg aaagaaattt catgaagaac gtgtcaaagt
    19621 aggctataaa ggatttagtg aaatcaattt tgattgtcat ttagttgcag gtgatcaagt
    19681 ttgtttaaaa tacgatcatc aaggaaaaga atatgtggct tggtatgaat tttctcaaag
    19741 agtattgaaa gacgaagagg gtaatatcgt ttatcggaaa aaacgaggag aaacccattt
    19801 gccagaatta gaaaaagaag ccgttctaac attagatgag gaaaaaagta acttattagt
    19861 agagtctatt cggatgcact ataccgataa accaatggct atttttatgc ttacacctga
    19921 ttatgatcca tctaaccata tcattgttag tatttttctt agtcagttat ataaagaatt
    19981 atctacgcaa tgtgttaaga ctaaaggaga taaatgtcac cgcaggattc attttatttt
    20041 agatgaattt gggaatagtg tgacctgttt agcctaagga taaaagaggc tagttccaac
    20101 gaacattgga ataactttac tatcgagaaa tcaaatgacg gggtaacgcc ctgaagggtt
    20161 tccactgata ctgcgacatg cgttagtagg atgtgcatcc aaacgcgtat gaagctcgct
    20221 gaagtcggca gaagggtacc gtaaggaaag acacgatacg tatctaccac gcaagtgctc
    20281 tagaggtagg gtatgtacct gatatgtcgg gtgtcctaaa aagttcaata aggtgagaat
    20341 gatttgatta tctgacaaac ttgcgaatgg aatgtctgaa acgaataact catagaaatg
    20401 tgagggggaa gggggatacg tcctctttcc attgactcga ggttgagtaa aaatgtgttt
    20461 ctatgaaaaa ccttgcatgg ttaaaggcta tggagtagtc aacaggcaaa agcaagcacc
    20521 taagttgaaa tctatggata gatagtaagg aacaggggaa gcatctagca cggagggagc
    20581 ataccgaaga tgtgtttcta aggaaaaacc gaaatcggtt ataacttagg tgaatagatg
    20641 tgaaagtaga gccacgacca gtgaaaattt cgtaatgaaa ttggaggaac aggctcaagt
    20701 cgttatttat aatataagaa acctatttaa aatcatggat tctcgtaaga ctaaaaaggt
    20761 cacgaaccag aatggagcgt gataaaacca tgactaacac aaaaggaatt agcttaaaga
    20821 acaaacaact cagacacgcc gaatactacg acatgactga gactttcgat gaattgcacg
    20881 caatgagtgt tgcaaaatca aattttaaaa atctcatgaa attcattatt gatgacaata
    20941 atatctttct agcgtataga aatatcaaaa agaataaagg aagtgcaacg gccggatacg
    21001 atggtgtcac tataaaagac attgaaaaat tggcatgtgg tgacttttta actattatta
    21061 aaaggcgatt taaggattac agaccaagaa aagttaaaag agtagagatc ccgaaaccaa
    21121 acggaaaact tcgaccttta ggaattcctt caatatggga caggatagtc cagcaatgta
    21181 ttctacaaat tttagaacca atatgcgaag caaaatttaa taagcatagc tatggattca
    21241 gaccaaattg ttctacagaa aacgccatag cagattgtct tgctagaatc aatcaaagta
    21301 agatgaatta tgttgtagat attgatataa aaggattctt tgacgaagta aaccatacca
    21361 aacttatgcg tcaaatatgg acaatgggga ttcgagataa acaactcctt gtaattattc
    21421 gtaagatgtt aaaggctccg attgttatgc gaaatggcaa tgttgttttt ccaaccaagg
    21481 gtactccaca aggagggatt ttaagtccac tgcttgcgaa tatatgcctg aatgaatttg
    21541 attggtggat agctaatcaa tgggaggaac gaactgtcaa agagctgaaa caccactatc
    21601 gaaaaaacgg agcatgggat aaaggaaata tctattcgaa attaagaagc cacacaagtt
    21661 taaaagagat gtatctggta cgctatgcag atgattttaa aattttctgc cgaacaggag
    21721 aagaagccca aagggctttt tatgccacac aaaaatggct cagagaaaga ttaaagctcc
    21781 caatttcaat ggagaaatct aaagttacga acctaaataa agctgaaagt gaattcttgg
    21841 gtttttcaat aagactccac aaaaagggaa aaaagaaagt ggctttcacg tacgtttgtc
    21901 gcaaagcaga agaaaacatt aaaaataaac taaaacaaca gattaaaaaa attcaatacg
    21961 ctggagcaaa aaaaaatagg caccgagcaa taggtgacta caacatgatg gtcatcggta
    22021 tccacaatta ctatcggata gcttcacatt gtagtgtcaa tttcactgag atttatagaa
    22081 gtgttaggac agtaatagaa gcaagactga aagctagagg aatgacgaaa aatggaatct
    22141 atggacaaga tcatgaagga taccgtccgt atctaaagtc taagcaaatg agatatattg
    22201 aaggttttcc gatacttcca attggctata ttaaaaaaag aactccactc aacaagcgga
    22261 gaaatctaaa taaatatacc aaggaaggga gaaagctcat tcatcaagag caacaagcta
    22321 tagaaagctg gaaaatcaag tatcttgtaa aaggttttag taacaataag aaatacacgg
    22381 tggaattttc tgataacaaa atatctaagt ttatttccca aaaagggaaa tgcgctatca
    22441 caggaaacga acttttgatg gaagaaattg aatgccatca cattaaccca tatcatttgt
    22501 ccaaagacga tagttacaag aaccttgtca ttatctctag agaaatccat agattaatcc
    22561 atgcaactag tgaagaaact attctgaaac tgcttcggtt actgaaacta aatcactatc
    22621 aaattagaaa actaaacacg tatcgaaaaa aagcagctaa ttttccaata tcaatcaaaa
    22681 tgaattagat aggaaacaaa ttatattgta attaacgatg gaacgccgtg tgcggtgaaa
    22741 gtcgcacgca cggtgtggac cgggggaaaa gacggagata atatcaaact cttacctatc
    22801 ggaattgcca ccaatggata atatggaagg aatcatgacg gtcacagctg gccgaaacat
    22861 gttgtgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg gagaacaaaa
    22921 cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga cagatgataa
    22981 taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag agaacagtac
    23041 catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag atcgtatctt
    23101 aactcctgaa agaatttcta cttttttaga aggtgaaacg attgtactcg ctcctttaaa
    23161 acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc atggggaaac
    23221 gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg atatcaatga
    23281 tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa ttgattatga
    23341 ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg atcaattaga
    23401 agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tctcttgaga atgagacggc
    23461 taaaaaattg ttaattcaat tttacgaaga acaagcagca gatcgacttc aagaatatgt
    23521 tgaaaagttg atcaaaaatg aagaaatttc tgaaatgatt gggaaacggt tgttattgga
    23581 atctgaaaag gtggtgaaag aatgatcatt ggaggaattt tttctggcat agccgactgg
    23641 tggtcaggct tattcggaga aggggatgca aaccccaaaa ccgtaacgaa agtcttacaa
    23701 gattatggta attatctgca gtatcaagat gtgttcagtt ggatttgtaa tactttttta
    23761 tgggggttga tcaaagggtt atataatctc aatactttac tagaaaaaac catttatcaa
    23821 agctttgatt taaaagactt attgaacgcc gctggggtaa atgaactatt tcaaagtttg
    23881 attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg cttgaaattt
    23941 tttatgagta aacacccacc aaaagtcaaa aatatcttgg ttaatttatt tatggccatg
    24001 atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag taaaagcttt
    24061 tacggagacg tcactgcagc aaataaagca gataaaacgg gttcacctgc ttttcaaatt
    24121 attcaaaata atgtgatcga tgtcagtaca ttactagcaa ctgacccgaa taaagtagaa
    24181 tccctgccaa cagacaaaag aaatgcttta acagcgaaaa attttaaaac agcgaatatc
    24241 aatgggatta ttactcctaa acaagctaaa gaaatggcgg ataaagataa gagtggttta
    24301 tcagatttac aaaaggatcg gttaagagcg ttacaatacc gactggaact ggatgataat
    24361 ggaaaagaaa ttcctgtgca aatcagtgat gaaggcttag ccaaatatgt ttatacttca
    24421 gggtatcgtc gatacattag tagtccaggg attattttag ctggtgaatc ttctttggcg
    24481 gtggcctatt tatttatcct atttacgatc gttacttgca ttattgagct ggtatttaaa
    24541 aaattctatt tagtgattgc cgcgtcaacg gactttgaga caggacaacg gatgaaaagt
    24601 gctatcgaag atatttctca atccttcttg ttattggcat ttacaatttt agaattacga
    24661 atctatacct taatgctttc aggtattggt gatttgcatg cggcaggaaa gatcaatgga
    24721 ttcttgtatg ttgtcgcttt gattgtacta accattgcgc tattcaaagg ctctcaatca
    24781 gttacgaaaa ttttcggcgt tgatacgagt ctgaaaaacg gcagtaattc tcttcttagc
    24841 atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa ccttgcaagt
    24901 gctggtaaga gtggtttaag ccatttaaat gatattcgta aaaatggatt tggccatcga
    24961 aaagatgtta cccctgataa cggggaagaa caatcagacg ataaagctac aactgtacct
    25021 aatagtaaga aaacagtttc cgaaatgttc gggaaagcga aaaatggtgt ttctaaagcc
    25081 gcagaaggtc ttggttacgt caatgaacgt gggttaaaag gaacagctga agatcttaca
    25141 gaaaaagcca aagacacctt agaagataaa ttagacggca cgagagctaa agaattggct
    25201 gaagctgtga agaatccatc cgaagcacta gaaaaacaaa aagaaaaagc ttcagaagtc
    25261 aaagaaggac taaaagatgc gtatggtgaa ggacaagtca atgcagcagt gaaagctaat
    25321 acaacaaccg aaaaagaaaa tcaagaaggt atagtgattc cttcagtcaa agatcaccaa
    25381 gaagcgttgc actctaatga aaattcgatt gaagagaaaa attccataga agaaagaaat
    25441 tcagaagaag gaaaaaatgg acagttacca aaaactgatc cagaacaata tcgctataaa
    25501 cctaacagtg accttgaaaa tgagaagcga atttcagatt tatcaaaagt aaatcaagaa
    25561 gacaaagcag aaaatgaggg acagttacca aaaactgatc cagaacaata tcggtataga
    25621 tctgttaact ccgaaaatga aaaacgcatt tcagatttac caaaacgaaa tcaagaagta
    25681 caaacggatg aggattccat cgttctgcca aatgataatg gaatcgaagt tggaactgaa
    25741 aaatcaacga aaattggttt acctaaggca caaaatttac caatgaaaga tagtcgtttg
    25801 aatcaattca aggataatga tcaaagtttt gttcatcaat cacctgcagg tactgttcaa
    25861 atgaaaccaa gtcgtttgcc tgaaaatgga aatctgacaa ctgtttctta caatttatct
    25921 gaaagtggtg agacagggaa tgcgtccgtt caaaatgttg tgacaagaga agctgcacca
    25981 aaacctattc aaaacgttca tacaggttac aagccagcaa atgtggcatt tactgcacca
    26041 acaaaaataa atgtgacaag agaaactaaa aacaggaaca atgcattgaa agaacaacta
    26101 ttgagggata tcaaacagga tatcaaacca taaaaggcct acaagttagg ccttttcttt
    26161 atatgaaaag gagaaatcaa ttatggataa atatataaac atgaaaaacc ctccaagatt
    26221 tagtggagaa tggaagtttt tcgctttttt ctttttagat ctattaacgc ttggtggctg
    26281 ctttttttgg gcgaaaatgg tcaataatct tgtccactta ccactctttt ttgcactctt
    26341 gaattatggc ttatggattg cgatagcgtt agtgattgtt tggcgaccct ataataaccc
    26401 aggtcaacga caaatttttg cgattatcga gtctattttt tttgcggaca agaaccatta
    26461 tcatgcgtta gatcctaacc gaaattatca tggtacgtat aaggaggatg aataaatgcg
    26521 agtcaaagga caaaattcag ttgaaaggaa atccattttg cagtttggaa aaccagaaga
    26581 gcgctctatc ttggacttat ttccgtataa atcctgtaca gaagagggag tcttaattac
    26641 tagagaagat cattttcaac gttattatcg tgtagtttct acagacgtgg aaggattaaa
    26701 tgaacaagag aaactagaac gaatgaatca gttgaccacc gtcatgcgta cgtatgtgcc
    26761 aacaattaag ttagttagtt tgaccacaga gacggatcta acggaacaaa tcgtgcaaaa
    26821 gcggcagtta ttagataaaa atcgtatgga acagtcctta ggaagaaatc tacagcgatt
    26881 aagaaaatat gaaaagaaat tagtagagga aatccaggaa ttgaaacgag cagaacagga
    26941 acgccctgat ttaagcttct ttttcgtcat tgaaggaaag acaatcaagg atttgcaaac
    27001 cagaaaccgt cagttaatgc gatctagtgg cattttaggg ttaaaaccac tctacaaaaa
    27061 agaattgatc aagattcttt atcgaatgaa taatatgaat gatgagtagg aatggggaag
    27121 agagaatgaa atatccgttg atatggttag aaaaagatgt tccgaaagaa gagaccacta
    27181 gaaaatttcc tcacacttta agcctgggta agttgggaaa aggaaaatct tttaaccaag
    27241 taaagaatca aacaagaaga ggtgactgta catgttagaa gcaattaaaa cagctttttc
    27301 accacaaaat gatcaaaagg tacttgatcc attaaagcaa ctaggaaatc gggaaataga
    27361 aattttaaaa tccaaagggt atgatttaga ctttttaaaa caagtgatgc cacaaggtgg
    27421 attaaatttc gatgaagagt atgtgaccta tggaaatggt tattcacgat gtgtgacagt
    27481 ttatcactac acaaaaaatc ctacgctttt ttggttggcc aatctcatga acaatcctca
    27541 tacagtggca acactagatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa
    27601 tcgtgcctta gatgaattaa atgatcggtc agataatgaa cgaaaagcga ctgaccgaaa
    27661 taaaagcgcg gaagaatacc tagaactagc ggaatatgcc cgaaaattga cttctaatgg
    27721 ggaaatttct aaacaagtca aagtgagagt acatgtttac ggaaacacga ttcaagaagt
    27781 agatgaacga gcacaggaac tgatcgaaaa tctcaaaggg atggattatc gtgcagtgac
    27841 ctatctgcac aaaacgaaaa gagactggca atcgatgttc accatgttcg atgaacaagc
    27901 tgaatggttt ggaaatatca aagggcaatc actaccatcg ctaaacattg gcggaggttt
    27961 cccgttccac caccaaacgc taaaagatcc aggtggaaca caatatgggg aaaccatgac
    28021 aggcggcccg tttgtttgga atgccacgta taaaaatagt gtgcggttgt ctaccaatac
    28081 cttgcttcta ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc
    28141 ccatttagct gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccctt
    28201 tttaaaggaa tataacggca ttatggtgcg tttagacgga tctgacggga tgatcaatcc
    28261 tttagaaata tttgccacac gtacgtatga tgaagcttcc agtttatatg acgatggatc
    28321 agtcaaagat ttgaagatca atgaagcagc gtcctatcaa acgcacttag acaaagtggt
    28381 ctatcaggta cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac
    28441 ctatttaaat cgtttttatg aagagtatgg aacagtacca cgcggcttta cttcctcaaa
    28501 ttcgcgttca aatactgata cgcaagtcac aggtaaagat ccagaagctt atccgacgtt
    28561 caaagaattt ttagactttt taggacaact ggaattacca ggtgcttccc aagagaaaaa
    28621 gaatcgaaaa gaagaaatgg aatccatcgt agaatcctta tgtgagacgt atggcatgat
    28681 ttttgatggt catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat
    28741 tgactctatt acaggcttag atcgaggggt acaacaatgt caaatgtaca tggctatgac
    28801 gttgatttgg aatcaggcat tgattcaagg aagaagacaa cgacgtttaa ttaaagaaaa
    28861 acaaatggaa gaacaagact tcaaatgctt cttggcgatg attgatgagt gccataatgg
    28921 tgcgacctgt tctgcagtga aaagctgtgg gttgcaagta gtctaaaaac tattgtaata
    28981 actcttataa cgagaagtca aatgagtggg taacgccaca aagggcttcc actgatactc
    29041 cgacatgcaa caagtagttt atgcagacca acgttgtgtg aagctcggtg aagtcggctg
    29101 aaggataccg taaggtaaaa gaaatagtga ataccacgaa agttctctag aggtaaaggg
    29161 tgtatccgat aagtcgggtg tccaaaaaaa cttaatatgg tgagtatgta caaacgtact
    29221 gacgaactag cgaatgtact gtccgaaagg catagctcct agaaatggga gagacagtga
    29281 gcaaaaaatc actcattgtg ctaacttgtg gttgagtaaa agtcgttgtt atgaaagacc
    29341 atgctgcgtt acaggcgtag gagaagttag ggggcaaaag ctagcatcta agttaaggca
    29401 tgtggataga tataagggaa caggggaagc catcaacatg gagagtgcat actcgatgaa
    29461 gtggtagtaa gaaatatgtg aatgacaaat aacttacttt agtgatggtg aaagtagagt
    29521 cacgactgat gaaggttctg taatggaact ggaggaacag gctcgattcg tctgtaaaag
    29581 tcatgaaacc cgttcaacca tgggattctc gtatgactaa aaaggtcacg agccaattgg
    29641 agcgtgataa accatgacaa aagaaacaag tccaaaagat aggaagttgc ggcatgcaga
    29701 atattatggc atgacggaaa tcttcgataa gatatactcc aaaagtttga agggagataa
    29761 tttcaagaat ctaatgcaga ttatcatttc tgataacaac attgtcctag catatcgtaa
    29821 tatcaagaga aatcacggta gtgtgacaag aggagaagat ggagtaacga ttaaagatat
    29881 tgaaaatctc aaccgtaatg aattcgtaga aattgttagg aagcgattcg cacattataa
    29941 ccctcggaaa gtaaggcgtg tagaaattcc taaatataat ggtaaattaa gaccgttggg
    30001 tattccatca atatgggata gaatcgcgca gcaatgcatc ttacaagtat tagaaccaat
    30061 atgtgaagca aaattcaata atcacagcta tggtttccgc ccaaatagat cagcagaaca
    30121 cgcaatagca gattgtatgg cccgaatgaa tcaaagtcac atggaatatg tggtagatgt
    30181 agatatcaaa ggtttcttcg acgaagtcaa tcatactaaa ctcatgagac aaatctggtc
    30241 tatgggtata cgagacaaac agcttctctt aatcattagg aaaatgttaa gagcacccgt
    30301 agtacttcca aatgggaagt tgatataccc aacaaaagga acaccacaag gcggaatact
    30361 tagcccatta cttgcgaaca tcaatctcaa cgaatttgac tggtggatag caaatcagtg
    30421 ggaggaaaaa gatgccaaag agctaaaaca tcattttagg aaaaatggag cttgggataa
    30481 aggtaatatc tatcacaagc tcagaaagag tacaactcta aaagaaatat acctagtgag
    30541 atacgcagat gattttaaaa ttttctgtag aaattataat gaagcgaaaa aagttttcta
    30601 tgcaactaaa atgtggttac aggagcgtct aaagttacca atatctgaag aaaagtcaaa
    30661 aatcacaaat ctttcaaaag aagaaagtga atttttaggc tttacgatga aattagtgac
    30721 caaaggtaac aaaaaggttg ttaatacgca tataggtaca aaagcacaaa gcatgattaa
    30781 agtgaaacta aaaaaacaaa tcaaacgaat tcaaaaaagt cctaatagca aagatacctt
    30841 tatctatatt ggagtctata acagtatggt tattgggatt cataattact tcaaaattgc
    30901 gactcattgt agtgtagcaa tgcgaaaaat tgcaagagaa ctcagaccaa gttttgaaat
    30961 acgactacga gaacaaggaa tctcaaaaga aggacaatac ggaaagtacg aaggatacaa
    31021 gccttaccta cggtcaggac aaatacgata tataagaggg tttccaatat taccagtagg
    31081 gtatattcaa cacagacatc cgatgtgtaa aaaaagaaat attaataaat acaccgaaga
    31141 aggtagggaa gccattcata agaagcaaca aaccgtagaa gaatggaaga tatcgtggtt
    31201 gtgtagcaat ccagtgctta gtaagagagc aacgattgaa tttgcggaca atcgaatttc
    31261 aaaattcatc gctcaaaagg gaagatgtgc agtaacaggt gaggagctta tcttatctga
    31321 aatgcattgt catcatatta tcccttatca tgaaagcaaa agtgatagct atgaaaacct
    31381 agtgattgtt actgaggagg ttcatcgggt aatacacgct acacaaagtg aaacgattga
    31441 ggaacttcta aaatatctaa aattaaatcc aaaacaaaaa gaaaaactca atgagttgag
    31501 attaaaagtt ggtaacgaag aaatatctta ataaatattt gtaggacggg aaaacagaat
    31561 gtcttttata gacgagggaa cgccgtatgc ggtgaaagtc gcacgtacgg tgtggactgg
    31621 gggaaaagac ggagataata tcaaactctt acctatcagt atcgattaat ccagatttta
    31681 ttgaaacggt aaattatgtc acaaactttc aacgagaaat gcgtaaagta gaagcaatga
    31741 ctgttttagc tacacaatcc ccacaagaaa tggtgccaga aaatatgtct tctgataact
    31801 tctcgaaact gaagaaagtc tttgaatttt cttctacgaa gatctttatg cgtatggatg
    31861 aatctatttt acgtcatata gaaggattag ttggtaaatc aatgacaaac tcagaattag
    31921 aatccattcc tcaacaaaat caaggagacg ctgtgatcaa ttttggctca aaagaaacca
    31981 ttcgtgttca ttttagtccg aacaaacgtc aattaaaatt gtttgatgga ggaaaataat
    32041 ggaaaaccaa aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa agcgacgctt
    32101 atttttgtgg ttgtttggta cttctgcagg tttgattact attttgatta ctgtgttcgt
    32161 gacccttttt ctaatacttg cggcgggttc gattgataat tctgattcag attcaagtag
    32221 tggtggcgaa gcttttacag gagaatattc ggaaggctta ccgatttata aagaaatcaa
    32281 aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag cagtgaaata
    32341 taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt ttggtacgtc
    32401 ggcgtccgca agaaatgatc tcaactattt tgggattact tggtttgatg gttgtctgtt
    32461 tcctaaagga actgcgcgag gaatcggtgg tattgaaggt ggctggtaca tgaagtttcc
    32521 caactcaaaa gcagccttct cctattacgg ctttatggta gccacacaat ccaattttaa
    32581 tgcatgtgta ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggtggtta
    32641 tgccgctgca ggaattactg aagatagtcc gtattataca ggttgtatgt ccattatcac
    32701 ttctaataaa ttaaccgaat atgatgaatt cgccatcaaa cattggggtg aaggtggcaa
    32761 taacaacggc acgatcactg gtgaatggac gaatccattc cctggcagca gtttagataa
    32821 aagttccttc agtggtggtc aattatttgg aacaaatcca ggcggtgaat tccgtccgaa
    32881 tggcttccat gatggattag actttggttc agtcgatcat ccaggaagtg aaattcacgc
    32941 ggtgcatgga ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt taggtgcatg
    33001 tgtgatcgtg attaattacg acggtttgaa tatggtatat caagaatttg cgaatagtac
    33061 aggaaattct cgtgtgaagg ttggcgatca agtgaaagtt ggtcaagtaa tcgggatacg
    33121 agatacagca cacttgcatt taggtttcac tcgaatggat tggcgtcaag cacaaggaca
    33181 cgcattcaca gacgatggta catggattga tccattacca tctttgaata gcagcaaaaa
    33241 atagatcagg agaaaaaata tggaaaataa aactttaaaa atcgtcttaa cagctgcaat
    33301 tagcgttagt ttaatcggca atatcgcgtt gttttggcag ctgaaagaag aacaagagac
    33361 aaataccttt aaaattgaag aagtggctaa aaacgcgtct gagaagctca aaaaaaattc
    33421 ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga
    33481 aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca
    33541 aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa gtagaaatga
    33601 attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc
    33661 agacgatgat caccaacatg atgattacgg aagttactcc tttacattag gtgacgtgca
    33721 aacttatgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta
    33781 tacaatcggc aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac
    33841 tgtcactgaa aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg
    33901 ttaggaggaa gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa
    33961 gttatcttca atggctggga atctttatga ttctcggctt tttgattctt gctagtccga
    34021 tttatacgaa tcagaaggta gatataccag atgaggtaag cacgaacgcc tttttaaaca
    34081 cgaaagagct gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
    34141 ttgcgatcga tgatgaagat caaagcttgt taaaagaact ggctaatctt aattatcgcg
    34201 tagaagtaaa gacagctaaa ggggattatc aagcaatacg agccaaagtg atcaaggtgt
    34261 ctgatgatta ttttgcagtg aagctaacgc atgtaccaga agaatatatc gccatgcgtt
    34321 taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga
    34381 tttattacgt gcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac
    34441 acgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagct caaaaacaaa
    34501 tggaatcttt ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc
    34561 aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc
    34621 agcaagaaat tgaaacatcg aaaacgcaac tgaaagatca agaagagatc attcaaaaac
    34681 ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa
    34741 aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta
    34801 aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaaca
    34861 caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat
    34921 ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag
    34981 gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa
    35041 ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac
    35101 ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg
    35161 tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt
    35221 ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac cttaattttt
    35281 aaagagcata aggaaacgtg atatcacttt ctcacgaaag gtgttaggta ggctgtgcct
    35341 accacgtttc taagaaagat aaatgatatc aaaattttgg atttgtgtgt agatttgtgg
    35401 gtaggatgaa agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa aatcaaaaaa
    35461 tgtgtaggga tatgtgtagc tacacatatc cctacacata ataaatttgg aggaaaatat
    35521 ggacttatta attagaaata ttgaagaaaa gtatattaat aaaattgaca aacgttgtga
    35581 agaacttacg gtcaagactg gcaaaaaatg gagtcgaaat caatacctaa aagttttgat
    35641 cgaaaatgat tttgatcacg ctcttctgaa ttataaaaaa gaccaatttg atcggttgtt
    35701 agaaaaattt gtggatattc aaacttataa tacgaaaact ttagaagaat atattgcaac
    35761 aaataatcaa ttaattggtt tgttaattga ataataaaaa tagtattgtg aatttataaa
    35821 aggaggaaat caattttggc aaaagcaaca agaaaacatg tatatcttta cgatatgcat
    35881 atcgaagtac ttgatgaaat aatttctact catgaagata tatctaatta tagtgaagca
    35941 gtcagacatt tgtgtatgat gtatgatcca aaaatcgaag aaaaaataaa tccaattcaa
    36001 attaaattga atgcaatggg aaaagaaatt tctattttaa ccgaaatggt ttcagagttt
    36061 gcggatcttc atttgaaaga tacaggaata ctaattggta caaattcaac tgtttatcaa
    36121 gaagcagaag aacgtgtgga aggaaaaatt aaatccaatc aaaccaaaaa atattcttct
    36181 aagttaaaga aaccagtgaa tgaatctgtt cataccagag tagatccatt tgatccaacg
    36241 aagattctaa aaagataaaa gtagaaggag aacgtgatga ataaatcgcc tgcagttatt
    36301 cttacttgtc aatttacctt gcccaataaa aaatcttttt caacatacat tgattatatg
    36361 acaagagaaa aagcactgga agaaatggaa cgacgtacgc ccgaagaaga acaggaactg
    36421 caacgaatca agaatgcttt agaggatttt tatattcctg aaggagaaac tttttcggaa
    36481 agtaagacgg ataaagaatt cagtgaaacg tcgaatgaag ctcaatcgtt gatggaacat
    36541 ggaatcaatt ttgacaagtt agaggaacaa gattttacca aatatctttc ttatatgacc
    36601 cgtcactatg cgttagaaca aaagaaagaa ctgacgcaaa gggaacaaaa agaataccaa
    36661 cggttgaacc aagcaatgaa aaaatatcaa acagagccac ataaaaaagc ttcaggaaat
    36721 cttcctggag ttttttctat ggcttcagac gaagtgaaag gaagtgatct gaaggagatc
    36781 aaacaaatct tccaaagagg acaacaaaaa ggctcaatca tttatcaaga tgttatctct
    36841 cacgacaatt cgtatcttga aaaaatgggc ttgtacgatc ccaaaacaga taccttagat
    36901 gaagaaggtt tgaaagcagc tggaagaaaa atgatggaga cattattcga gaaagaacaa
    36961 ttgaatgaaa caggttactg gatggcaacg attcaccgta acactaagca tatccatatt
    37021 cattttgcag ttgttgaaaa agaaaatacg cgtaaaccgt atactgagat tgaaaatggt
    37081 gtgagttata tcgtaccgaa ggggaagaga agacaagcaa cattagacgc aatgaaaacc
    37141 aattatgtac atgagttaga acgtttctct tatgaaaagg atcgaatgat cggtatggag
    37201 aaacgaaatt tattgacacg aaaaagtgat ttgcgtaaca ccttaacaaa agtagtaaaa
    37261 gaaccagcac gttacgatcg acacgccatg aacttattaa atgaagtata taaaacccta
    37321 cctgaaaaac gttcggaatg gaattatgga gatgaaaatc gaaccaagct ttcaccattt
    37381 acaaggggaa aacttgatga gttaaccaaa tatgttttaa caaaagaacc tggatatcaa
    37441 gaatatatac agatttcaaa agcattgaaa gaagaagcca aaagccttta tggggaaagc
    37501 aaaagagaaa caaagaatgc agaaaaaaac gccctctttg atttaaaaaa gcgcacagga
    37561 aatgcgattt taacagaact gaagagacag gatagtcaaa taaaaccatt aatagaaagt
    37621 gtcaatcaga tgcaatctat ttacaatgaa cgatcctttc gaacactcaa acagttgcca
    37681 acaaatgaat acaagtatag ttatcaagaa ttcgtaaagc gtaggaaaca attccaacaa
    37741 agagttgtca cgcagcgaag cttgcgtcaa ttaaatcgaa caattaatgc acatgaagaa
    37801 aaataccacg caagtaaagc atatgaagag caacagcgaa gaatccaaat ggaacaagaa
    37861 agacaatagg aggaagagaa atgaaacaat atttagatca atggaaagtc atagaaggat
    37921 cactaaaaga agaacgaatc gaacaacttc ctgattgttt agaaaaagag catttgtttc
    37981 agataagcga aatgttgaga aacgaacagt ttgatccaaa tcaattttta gttgttgagt
    38041 acccagctac tggtgtgtat tgttgtaatc atgtgaaggg tgaaaaatat tttattattc
    38101 aagaatacga aggtaagtta gcgccttatt atactacttg ggaaataaat gaagagggaa
    38161 tcaataattt cccatgtgaa agcattgaag agtctatttc tctcacggaa tgttaaataa
    38221 atagagacgt aaaagagtcc tgaacgtgat tgtccaggac tctttttcaa tgaattgttt
    38281 tccgccaatt cactgagtgg tgcgattcaa ttttattata tcaaaagaaa gtgagattcc
    38341 aaagtgaagg gaggaagaag aaatgactca gacaacgatt ggtgttgtgg ttgaaaaaag
    38401 cagggataat ctcgtttttg tgacagaaat tcaaacaggt agagcttttg tagtcacaga
    38461 caaagctgca aaagcgtatc aaaatggcaa tattttaacg ttaaatctga ctacgaagat
    38521 gttcgttgat gcagtagaaa attatccatt tgtttagaca aacgatattt tacaaggagg
    38581 aaacggacat ggcagaagaa aaagaaccac tagataatag tcgtttggga aagtccaaaa
    38641 gaaaattagt acggttgcaa aacgaattaa ataatcaatt tcaaaagcta tttgatcacc
    38701 aaaaattgac aaatgggcaa ccaatgaatg acaaacgaaa tggtcgttca tggtttaacc
    38761 agcaagaaaa aatagaaaat aaaatacaaa gtttgagaga agagatcaag caacaagaaa
    38821 aacaggtaga aaaacttgaa agacaagaag agctaaagga aatgggttat aacaagtacg
    38881 gtggcctgga catgacgatc gaaaatattc caagaatcaa agaagaaatc gaacgctttg
    38941 aaaaaggtga atcgactttt tcagctgcca cgatccgaaa ataccaacga aaattggaaa
    39001 cattagaaca gctgaaagaa aaatcagaga aaggaaaaga aaatcttctt cctgaagtac
    39061 aagcgatcat tgacagcgga cgagtgacgc aatggaaaaa gaatccaact atctattttc
    39121 tgaaaggcta tcgaaaagtc gctttagaac tcaatgaaaa cgggggattt gaggaatcta
    39181 aaaaatacca agccacgtcg gaagaagaaa aggcagctgt tcgtgaatta cttgaagaag
    39241 ctacacctac tgattctgtg aaggagaaag aaaccgatca tttctatcga gtagaattca
    39301 acgaaaatga tccagcaaaa gggttgaaaa gttatggtgg ggagatcgta acactagaat
    39361 tgatcgatca actcaaaaca ctggataacc aatacgccgc agaagcaggc tattataaat
    39421 tctattttga agaagtgaag aatggagaag tagtcgatca gatacgaatg gatctagggg
    39481 atggcgtcaa tagtaatcac cccatctatt tagagttagc tagattgtgc gaacaatcac
    39541 ctgaaaagga acaagaacaa acagtcaaaa atgaacctat ttcaaaagta caaaacgaga
    39601 ttaaagagac aaaaacaaaa caaaagggtt atcaaaaacg aacggcagaa gagattaagc
    39661 aagaagtcaa atcactttcc caacaggcgc ttgatgcggt gaaaaagcat actcattcac
    39721 ctgaagacgt caaagaactt ttgaatttta tgtctcgttt tccagaacga tcctttagaa
    39781 atcaattatt gatcgaggaa caatttccag gcgcaacggc ttgtttaggt cgtgccgcgt
    39841 tgaaaaaaga aggaatctat atcaaaaaag gagaacaagg gaacaagatc tttgtcagaa
    39901 aaacggtcaa aggattttat gagaaaggac gtggctttgt tcgagaaaca gaggctacac
    39961 cagaagataa aaaacgaatc gaaagtggtc aaatcgaagt gatcaaaaaa ccttactaca
    40021 cgattgaaaa agtgtttgat atcacacaaa cacaattgaa acctgaagac tatccgaaaa
    40081 tcttcccgaa ccgcgtcttt gattttcaat tggatcaaac agggcagaca gaacttcaag
    40141 ttgggattca agcggtagcc aaaaatattg gaattcaaat tcgagatatg acagaaagtg
    40201 aagtctatca aagagaatta ggacaagcac gtggggcgta cgttcgtaac gaatttactg
    40261 gcgaagaaga aatcgtcatg aatactcgaa atactcctac gcagcattta gccacaagta
    40321 ttcacgaatt agcccatgct agaatgcaca aattttcaga attagatact gcaacaaaag
    40381 aactacaggc agaaatgacc tcctatattg tgtgcaagca ttttgggatg gacacctcgg
    40441 agaaagctat tccttatatt gcgacatgga cgaagaatag tcagacgttg gatgataagg
    40501 aagctgcgga acgtgggaaa atcatgaatg atgtcagtcg tgtcgcaaat gaattcattc
    40561 aaaccatttc taatgaaatc aatcaataca gagaaagaga accagagatc cagccagcag
    40621 aagtgaagca ggagcataaa aatactgtag aaacggcaga acaaaagaat attgtgcccg
    40681 ttggctcact ttggatcgac aagaaagacg ggaaagtcct ggaagaagac acgggccgaa
    40741 cgttacattt gaaggatgcg gcgtttattg aagatccgca gcataatcaa gtgattttaa
    40801 aaggaaaaag ttatggaaaa gagatccaaa cggcgttacc ttcagaagaa tttgcgctag
    40861 gaaatccatt ccgattttca cttgagacag tgtcagaagt ggcaaaacga aaaaatgaaa
    40921 tctattatga acaacaattg tcacaaaaag aacgcgtata aaacaagcaa gaaagactat
    40981 ctgaaaaatg gatagtcttt tttcttttgg gaggtttgaa aatgacagag aaaaaaatga
    41041 gtctcattga tcgatgcaag caaatggata ttgtggattt tgcacgaaac aatggtttag
    41101 cggtgg
//
LOCUS       DAJSBC010000020        41049 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSBC010000020 DAJSBC010000000
VERSION     DAJSBC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.020"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(335..1117)
                     /locus_tag="ONT65_001076"
     CDS             complement(335..1117)
                     /locus_tag="ONT65_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328723.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1182..2018)
                     /locus_tag="ONT65_001077"
     CDS             complement(1182..2018)
                     /locus_tag="ONT65_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2328724.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2036..3445)
                     /locus_tag="ONT65_001078"
     CDS             complement(2036..3445)
                     /locus_tag="ONT65_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2328725.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3459..5282)
                     /locus_tag="ONT65_001079"
     CDS             complement(3459..5282)
                     /locus_tag="ONT65_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2328726.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6125..6451)
                     /locus_tag="ONT65_001080"
     CDS             complement(6125..6451)
                     /locus_tag="ONT65_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328727.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6497..6859)
                     /locus_tag="ONT65_001081"
     CDS             complement(6497..6859)
                     /locus_tag="ONT65_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR2328728.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6896..7111)
                     /locus_tag="ONT65_001082"
     CDS             complement(6896..7111)
                     /locus_tag="ONT65_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328729.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7238..7573
                     /locus_tag="ONT65_001083"
     CDS             7238..7573
                     /locus_tag="ONT65_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328730.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7649..9646)
                     /locus_tag="ONT65_001084"
     CDS             complement(7649..9646)
                     /locus_tag="ONT65_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328731.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9744..10019)
                     /locus_tag="ONT65_001085"
     CDS             complement(9744..10019)
                     /locus_tag="ONT65_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328732.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10251..10985
                     /locus_tag="ONT65_001086"
     CDS             10251..10985
                     /locus_tag="ONT65_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328733.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11024..12166
                     /locus_tag="ONT65_001087"
     CDS             11024..12166
                     /locus_tag="ONT65_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2328734.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12261..13652)
                     /gene="sufB"
                     /locus_tag="ONT65_001088"
     CDS             complement(12261..13652)
                     /gene="sufB"
                     /locus_tag="ONT65_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR2328735.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13657..14127)
                     /locus_tag="ONT65_001089"
     CDS             complement(13657..14127)
                     /locus_tag="ONT65_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR2328736.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14114..15349)
                     /locus_tag="ONT65_001090"
     CDS             complement(14114..15349)
                     /locus_tag="ONT65_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2328737.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15346..16632)
                     /gene="sufD"
                     /locus_tag="ONT65_001091"
     CDS             complement(15346..16632)
                     /gene="sufD"
                     /locus_tag="ONT65_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR2328738.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16645..17418)
                     /gene="sufC"
                     /locus_tag="ONT65_001092"
     CDS             complement(16645..17418)
                     /gene="sufC"
                     /locus_tag="ONT65_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR2328739.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17615..18463)
                     /locus_tag="ONT65_001093"
     CDS             complement(17615..18463)
                     /locus_tag="ONT65_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2328740.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18487..19173)
                     /locus_tag="ONT65_001094"
     CDS             complement(18487..19173)
                     /locus_tag="ONT65_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328741.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19166..20200)
                     /locus_tag="ONT65_001095"
     CDS             complement(19166..20200)
                     /locus_tag="ONT65_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328742.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20263..20519)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20595..20933)
                     /locus_tag="ONT65_001096"
     CDS             complement(20595..20933)
                     /locus_tag="ONT65_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR2328743.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20935..21291)
                     /locus_tag="ONT65_001097"
     CDS             complement(20935..21291)
                     /locus_tag="ONT65_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR2328744.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21297..22484)
                     /locus_tag="ONT65_001098"
     CDS             complement(21297..22484)
                     /locus_tag="ONT65_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2328745.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22675..23520)
                     /locus_tag="ONT65_001099"
     CDS             complement(22675..23520)
                     /locus_tag="ONT65_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2328746.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23754..24719
                     /locus_tag="ONT65_001100"
     CDS             23754..24719
                     /locus_tag="ONT65_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR2328747.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25096..26139
                     /locus_tag="ONT65_001101"
     CDS             25096..26139
                     /locus_tag="ONT65_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR2328748.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26201..26665)
                     /locus_tag="ONT65_001102"
     CDS             complement(26201..26665)
                     /locus_tag="ONT65_001102"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR2328749.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26911..27858
                     /locus_tag="ONT65_001103"
     CDS             26911..27858
                     /locus_tag="ONT65_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2328750.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28069..30141)
                     /locus_tag="ONT65_001104"
     CDS             complement(28069..30141)
                     /locus_tag="ONT65_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2328751.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30074..30451)
                     /locus_tag="ONT65_001105"
     CDS             complement(30074..30451)
                     /locus_tag="ONT65_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR2328752.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30580..31158)
                     /locus_tag="ONT65_001106"
     CDS             complement(30580..31158)
                     /locus_tag="ONT65_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2328753.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31290..32582
                     /locus_tag="ONT65_001107"
     CDS             31290..32582
                     /locus_tag="ONT65_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2328754.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32642..33760)
                     /locus_tag="ONT65_001108"
     CDS             complement(32642..33760)
                     /locus_tag="ONT65_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR2328755.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33774..35195)
                     /gene="arcD"
                     /locus_tag="ONT65_001109"
     CDS             complement(33774..35195)
                     /gene="arcD"
                     /locus_tag="ONT65_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR2328756.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35441..36562)
                     /gene="mnmA"
                     /locus_tag="ONT65_001110"
     CDS             complement(35441..36562)
                     /gene="mnmA"
                     /locus_tag="ONT65_001110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR2328757.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36966..37565
                     /locus_tag="ONT65_001111"
     CDS             36966..37565
                     /locus_tag="ONT65_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR2328758.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37703..38989)
                     /gene="efmA"
                     /locus_tag="ONT65_001112"
     CDS             complement(37703..38989)
                     /gene="efmA"
                     /locus_tag="ONT65_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR2328759.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39260..39604)
                     /locus_tag="ONT65_001113"
     CDS             complement(39260..39604)
                     /locus_tag="ONT65_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR2328760.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39741..40886)
                     /locus_tag="ONT65_001114"
     CDS             complement(39741..40886)
                     /locus_tag="ONT65_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2328761.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11801 a         8636 c         7178 g        13434 t
ORIGIN      
        1 atttctacat agttatcttt agaaccattt cagatacata tgaaacaatt acatatgatt
       61 ttttcataaa aaaacagtac tgtctagtag tataaaatat acaaatttct taaaaatttt
      121 ttcactaaaa tcactacctg aaacagtcta aaacacttat atatcaacgt ttaatgaaac
      181 tatctccgtt tcatttcaga tacaaataaa tatagtcggg ctccaaatcc gaaaaaaaat
      241 acccccagta gcttatccta agctactgga gatgtataag tacggtcata taacaacggg
      301 gtagggtaaa taaatgaaaa agatggaaga atcattattc aataactttt ttaaatactt
      361 cgttgtaatc agttacctgt ccgttcttaa ataccacaga ataatctttt ttcccatttt
      421 caagtaaccg aagtggaatg tctttaccaa cttgctctgt cactgcatta ctgatacctg
      481 ttagattagc taataaatct gataaagatt gagcatctgt agaaatctct gtatcagtat
      541 ttatcgtgat tcctttatct tcatttacaa gcttccaagt aggtggtata gctgtgtatg
      601 tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc agtatctact tctgtctcat
      661 tactcttaga tgaatcgatt ctttcatcca tcgtctgctc cgtaactgct tctgtctcat
      721 tattctcacc ggaaactctc ttcacaccca aacttatata ttgtccatct ttagtgtgca
      781 tagttaaaat tcccacgctt ttactaataa attcactata accagttctt tcaatagaga
      841 cttcaatgta tttcacacta tcatactcaa aagaaaaata gacatgtaaa agcattttgt
      901 tgtttacatc tacataataa tcctttgact ctacttgaga ccttcctata cggaatacag
      961 ctttactgtc tgatatctct ataccagcat ccttattatt aatctgccaa tctccaagta
     1021 gctcttctgg caagctagta gtttcttgtg aacactttac cacttcactg gtagaggtcg
     1081 aaacattcac ctccgcactg ttagaagaat tttccaagga atcaggagcc ttagcacaac
     1141 caacaagtct ttatatattg tgcagccgat attttcttca ttcagtacac ccttgaaata
     1201 tgcagtgtta gataaagcag ttcgtcctga tttgctgagg cacccatctc ttcttgaacg
     1261 tactccttga ttttcaaggc cgcccgatac tctttcggaa aacgctgctt cactgaagcc
     1321 agcaaaaaat catccttctc cggcaactct tccttcttaa tcattcgggc aataaaaaac
     1381 tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt tatcaacctt tttttcggga
     1441 aacagatatc ctagcatagc caagatatcc tttagtgctc ttaccgagag catggtttct
     1501 tccatatttg cttgttcact ttgggcattt acaatgtgaa acgcaatgtt tccagcctcc
     1561 tcttctggca actgaacatt catttttttg ttgatcaatt ggatagcata gcagccgatc
     1621 tcaaattctt ttgggtaata cttttgaatt tcaatcagca ggcgattttg aaaaacaatg
     1681 cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa ccaaggtaaa aaatagactt
     1741 ttattcagct taaagtcaaa cttggcgttg gcataatcga taattcgctt gacgcaaata
     1801 attagatcat ccggagcagc ctcaaacagt ttaatgtaat ccttttttag agcatcagaa
     1861 agtgaatcta atttaaatac tttttggatt ttgtcagccg gaaccaaatc gcctttcttt
     1921 agttgaaatc caatcccttt ccccatcaaa attacttctt tgccttgctt atctaagcaa
     1981 agaattaaac tgttgtttaa cgttttaatt actctcatta gattcaccgt tctctctaag
     2041 ctaagccatt tcttgcgatt actttttgat accaaaagaa gctttctttt tttattcgtt
     2101 ttaattcttt aagattctca tcggttcggt caacatatat aaaaccataa cgcttattga
     2161 agccttgatg ggtactaaca acatcaatcg ctgaccaagg taaataacca aaaacatcta
     2221 caccatctgt taaagctgct tgaatagcca gaatatgttt ggctagataa tcaattcgat
     2281 cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg tgccccatag ccattttcag
     2341 taattaaaat aggtaaatca tagcgatcgt acaatttcct caatgttaat cgtagtccag
     2401 tagggtcaac tacccagcca tatggcgttt tatctacata aggattttca gctgcccggt
     2461 agacaccttc ttctcccaac atgatctgtt gatcccccgc tctagcggaa acatctgatc
     2521 cgtcattttt gctggcagcg atggtggcag ttgaataata attaatcgca ataaaatcgg
     2581 gtttaccgga ggataaaatt gcctgatcct ctgcctcaat tttaggatat aatccacgat
     2641 cattcaaata gttttcaaac aaataattgt attttccccg aactgctaca tctaagaaag
     2701 accagcctct caatgtttcc caattatggg ctgcaattgc atcttctgga ctggaggtag
     2761 cttgatacat agccgttaaa ttgattgctg gaccgatttt tcctttaaga ttcaactgat
     2821 gatacaagtg aattactcta gcttgagcta ataaaacatg gtgattaatt tgaaagagtt
     2881 ctttttttga aggcagctct ccacttggtg gcaaaccaat cgctccagga tgaaggatca
     2941 tcgtgttttg ctcattaatt gtcagccaat agttcacctt gtcaccatac tcagtaagca
     3001 atacctgtac atagtccaca aaataatcga tagttgctcg atttaaccaa ccgccctgct
     3061 cctctagaaa ataaggtaaa tcgaaatggt acatggtgac aatcggttcg attccctgat
     3121 tcaggcattc atcaattact tgatgataaa aagaaattcc cttttgattg gttttattat
     3181 tttcaatatc cataattcgc gtccaagcga tggaaaagcg aaaagctttt aagcctaatt
     3241 ctttaaacag ctgtatatct tctttatagt gatggtaaaa atccgaggca acttcaaaat
     3301 tagctacccc ttctgtatgc tgatacttgt caataataga cggttgtttc ccatcctcct
     3361 gataggcacc ctctgcctgg taggcactaa tggaggcacc ccataaaaac ttctctggaa
     3421 atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt aactaatttg catcaatggt
     3481 tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg cgtaattttc actgttggtc
     3541 actaccacaa tcactgtatc atcgtagcca gcgtttctaa tggcagcgaa atcggcgttg
     3601 attaataatt gtcctgctga cacctgatct cctgctttta caaatacttc aaagggttcc
     3661 ccttttaaat tcacggtgtc gatccccaca tggatcaata attcaatacc ttctttagaa
     3721 cgtagaccaa tcgcatgttt agattctaaa atagtaatga cttctccctc aacaggtgaa
     3781 taaatgctgt tttcttctgg atgaatggct acgccctttc ccaatagtcc cttagaaaag
     3841 acagcatcgt tcacttcaga aataggacac acggttcctt ttaccggatt ttcaacaatg
     3901 actgcttcgg ctattgccgt ttgctctgat tcatcagaag tatcgtcaaa tccaattaga
     3961 taagcaatga taaaggttgc aacaaaggcg attacacacg taataattgc gtgaacaata
     4021 ttcattggat tttctccaat aaaggctggt aaggctgcta agccagggga aacaaaggca
     4081 tatctgacta aaccgctcaa gccagcatac aaaccagcaa ttgcaccacc aatcatggaa
     4141 gcaactaatg gttttttcag cttcaaggtt actccataga gtgaaggctc agtaattccc
     4201 attaaagcag tgatcccagt ggacacagct agctgcttca actctttgtt tttagttttc
     4261 gcggctacag caaaggtagc acctgattga gcaatattag aagccagcat tcctggccca
     4321 ttgatcattt cagaacccgt actgctcaat tgcatagtag cgattggcgt cattgcccaa
     4381 gctgtacccg taaccaccaa aaatggctgc aaaccaccca tcaaaaatgg aattaaccaa
     4441 gctgctttgc cgttaatcca ttcagccccc attgctataa gatcgttcag caacgttcca
     4501 aacggtccaa tgacaaccaa cgctaaaggc cccataatta aaaataccag catcggctta
     4561 gtaaagaatt taataaacga aggcgaaatt cgatccgcaa agggctctac ataggacatg
     4621 atccaaacag taaagataat cggtaaaact gatcccgcat aatctgcagc taataccgga
     4681 attcctagaa aagaaacggc atcgccagta gaaaaaagct gtgaaatatt tggatgaagc
     4741 aaaatgccag caacagtaat cgccaaaatt gggttgacat caaactttat ggctgcccca
     4801 taggctaata gtactggcat aaaataaaag gctgcatcag caatagtatt caaaatgatg
     4861 taagtttgac tactgtctga tagtacacca gtcaaagtca agacggccaa aatggctttt
     4921 accatcccgg cacctgttat agctggaatc atcggtgtaa aggtggtaga aatgacacta
     4981 atcaatcttg aaataatact ttcttgattg tctgaagagg ggcccttttt aattgaagtt
     5041 cccacttcct tttgaatttc cttgaaggct gtttggactt cattgccaat caccacttgg
     5101 tattgtccac cgctatccac cacactaatt acaccaagtg ttttttctaa ggctttcgta
     5161 tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg tggcacaatg ggttaagctg
     5221 accacatttt cttttccccc aacctgttgc aatacatttc ttcctacttc tctgtaatcc
     5281 attattctct ctccttaaag cagtaaaaaa gaccaagtca aaccacgccc caatcaacat
     5341 tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt aacatgccaa taacgttatt
     5401 ttgctgtaat ccaataatag cacattatga tagcgcaatc aacagggaat ttaatttttt
     5461 atcttttcgt ttgaccgtat agggtaatat aacagttaga tcacatcaac tagggtatat
     5521 ctgcgtatac ttactgctaa aataagtata tctaatatta actataccag taaaaccatt
     5581 cctacataac cagcgggaaa ttcccaaaat cgtgaaagca tcaaaaatgc atataacgga
     5641 tcacacaact tcaacaattc tgctcttggg aaaaattgat gaatttggtg agtaaaggat
     5701 ggaataaaca gcattccaac aatataggca acaatagtga tacttgtttt tttggtaaaa
     5761 atcagtaagc aatagccaat ttcaatcatt ccgagatata aaggtatcga tagaatgatt
     5821 tgggctagta ttcttaaaaa ttaactagca aatgtttaaa ctgatttatt tctaatctac
     5881 atgtagtaag tatttcaatt ccaccaatta tcaataaaaa taccaaacat atccataata
     5941 attttttctt tttcatttct tttatattgt tataacagtg aataacataa aaattcccga
     6001 caatcattgt aatatttttt tgtcacctgt attagtattt aatttatagg aaactataaa
     6061 aactccctag tgaatttaca cctttgggag ttcctgtttg gacggtatgc ataatacatt
     6121 taaatcaatt taacattaac acctcagcat atggtatctc cacatatata cggtctttct
     6181 ttggatagta ctcaatattt atgttgatag cacttccatt gtctaatata gctttagccc
     6241 ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc atcacatgat cgatttaagt
     6301 tgtactgttc ttgtaaaatc tgtgttacca atggttttga tgacttctct aatccagatg
     6361 tacctgtcat ttttgaaaaa ataataccta taatagccaa gacaacaata cctataatga
     6421 caaacttctt gttcatattt actttattca actttttatc tcctatctcc aaaataattt
     6481 gctcttatcc ctcttatcag tacattgaca tccctaaaag tacaccaaag attgctccag
     6541 ctacccctgc tatcatcaaa atcgtaccca ttttctcatc tttttctcga tataagtagc
     6601 ccataataac agcaacagca ccaaatacaa caggaataaa tgctaacgat actaccatag
     6661 aaatccaacc aataatcaaa tacagcttcc ctgtctcttg tccatgtacg atttgttcct
     6721 tcatttcatt aattgtctgc tcagtacttt tctttactgt gtctgttccg gttgtctcat
     6781 ctgtttcttg tagtgctcca caaaactggc aatacttact ttcatctgtt atttctttgc
     6841 cacattttct acaatacatg tcttctcctc ctaaaatcat tcaattgctt attagttaac
     6901 tgtttacttt attaaataca tcagaaaaca gctcattcat tggtacactt aatatctcag
     6961 aaagcatctt catttgatag ggtttaggaa tcgttctccc tgtttgccac gaacttacac
     7021 attgctgtgt aacattcatt ttttttgcta actccgtttg agaaagtcct ttttcttttc
     7081 tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt attatataca
     7141 acaatatgat gtatttcaac tcataaaata taaaaaactt caaataccat tacaaataca
     7201 acaacttgtt gtactatact gagatggggg tgtattaatg aaacagaaac aatatgcaaa
     7261 ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa aaagaattag ctgaaaaaat
     7321 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagagc ctaacctcga
     7381 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     7441 tgtcgtagat tatgaggaag agtttttggc tctctatcgt tctcttagca ctgaagatgc
     7501 agttcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     7561 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     7621 ataagattaa gagctatgga taacctgtct agtttaatac aattgggtct tttctcttcc
     7681 ttactctccg tccatcatct cttgttacac ttgtaaattg atacagagga actatcccag
     7741 ctcctgataa tatcaattct tttattgttt catctatccc tgcccctttc gctaaatata
     7801 ttaaaaaact actacttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     7861 caagagtgaa tccttgttta acaaagtagc ctctgttttt tattaaagca tttttcacgc
     7921 tgttatcccc aacattacgt aggcgttcac aaacagtgtt aataagttct ttctctgtca
     7981 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     8041 attgtttttc cttgctgagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     8101 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaattt
     8161 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     8221 cgtctaatcg atagacattg cttctcttat gattaaagtt tcctaacgct ttctcttcct
     8281 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     8341 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     8401 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     8461 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     8521 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     8581 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     8641 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     8701 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     8761 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     8821 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     8881 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     8941 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     9001 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     9061 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     9121 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     9181 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     9241 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     9301 tatgaactaa attgatagat aaaacctttt ctacgcttaa gtatctacta tcatctttac
     9361 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     9421 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     9481 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     9541 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     9601 tttcttgatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtttcctcct
     9661 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     9721 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     9781 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     9841 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     9901 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     9961 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
    10021 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
    10081 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
    10141 catcgacata cacataatac ctcgataaaa aaggggtgtc aacagctttg taaaatttat
    10201 ttgcaacacc ccttttttga ggtatactaa ttttaggagg tgtggtcaat atgataaaac
    10261 gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata aaagctataa
    10321 gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac ggtcaattaa
    10381 ttgacaaaaa aactatctac gaatgggaga agggaaccta tttaccaata ccagagcgat
    10441 tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt ggcaatgtag
    10501 aagaatatat tttgggaatt attctttata gagatagcat tgtactagat ggtataacat
    10561 ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta cactctaatt
    10621 tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa tttattgcta
    10681 acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag
    10741 aagaattatt catcaatgct attgtagaag aatttgacaa taacattctt ttcctcacta
    10801 gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt caattgaaag
    10861 acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac aagctagaac
    10921 aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca
    10981 tctagtggta agtactcctt gtaaactaag aggagatgat ttaatggcaa aaatatcaaa
    11041 gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg ccaccgatcc
    11101 acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac aagaagctca
    11161 aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga aagcacctga
    11221 tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca gtgttaaagc
    11281 ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag catttggtaa
    11341 gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag
    11401 taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac gctacggggt
    11461 tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga
    11521 gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac tggatgcttt
    11581 taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat acacaggtat
    11641 gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta ataaagaact
    11701 acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc aatctccgaa
    11761 aacaagagct tctagacgag ttatctcttt agatactgag actctttcta tcctcaacaa
    11821 ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta gctctaaaga
    11881 gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg ttaatgattg
    11941 gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg ggtttagaca
    12001 tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc aacaacgatt
    12061 agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa ataatgcacc
    12121 taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga gctggtcaaa
    12181 atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat atatcaaggg
    12241 ttctagctcc tataacaggt ttacccaaca ctgccttcca tctcataact gatcaatcga
    12301 ttgagttcga ctgcatattc cattggaagt tctttggtga atggttcaac aaagcccatg
    12361 acgatcattt cagttgcttc agattcggtc aggcctcggc tcatcagata gtagagttgt
    12421 tcttctgaga tttttgagac tttggcttcg tgttctaaag agacttggct gttgtggatc
    12481 tcattgaatg ggatcgtatc ggatttcgac cattcgtcca tgatgatcgt gtcacattcg
    12541 atatgagaga tcgaacccgc actgtttttg gcaaaggtta cttgtcctcg atagtttact
    12601 tctccgccgt cttttgaaat tgatttagag acgatagagc tagatgtatt tggtgcattg
    12661 tggatcattt ttgcacccgt atcttggatt tggtttgctc ctgcaaaggc gatggagagc
    12721 atggtgcctc gtgcaccttt tccatctaag taaacacttg ggtatttcat cgttgttttt
    12781 gcacctaggt ttccgtcgat ccattcaacg gttgcgcctt cgtatgcttt tgctcgtttg
    12841 gttactaagt tgtacacatt gtctgaccag ttttggatcg ttgtgtaacg gcaatacgca
    12901 tcttttcttg taaagatttc tacgatggcg gcatgcaggc tgttacttga ataagtaggt
    12961 gccgtacaac cttcgacata gtggatgctt gctccttcgt caacaatgat cagcgtacgt
    13021 tcgaattgcc ccgtgttttc cgcattgatc cggaagtacg tttgtaatgg aacatcgact
    13081 ttgacgcctt ttggcacgta aataaaggtc ccacctgacc atacggctga attcagcgct
    13141 gctaatttgt tgtcggttgg cggtacaagt tttgaaaagt actctttgaa taattcaggg
    13201 tattctttca aagcagagtc agtgtcagta aagacgattc ctaacttttc gaattcatct
    13261 ttcatattat ggtacacgac ttctgattca tattgtgcgg aagcaccggc tagataagct
    13321 cgttcagctt ctggtatacc aatcttttca aatgtatctt tgattttttc tggtacgtct
    13381 tcccagtcac gagcgggttt gtcacttggt ttttggtaat atttgatggc atcgaaatcg
    13441 atgtctgaaa ggtcggggcc ccatgtttgc attggcattt tgttaaatgt ttctagagat
    13501 ttcagacgga attcaagcat ccattctggt tcgcctttga ttcttgacat ctcacggatc
    13561 acttcttctg tcaagccttc tcctgtactg aaaaccggtt cgacatcatc gtgaaacccg
    13621 aatttatatt cttctaattc tggtacgccc atgttctcac tcctcttcct tgtggtaatg
    13681 atggaaagct gcggtaccat cgttttccat tgcttttcct agtgctttcc aggcaagtgt
    13741 cgcacatttg atccgagcgg gaaatttagc tacaccacca agcatagcag catcgcctaa
    13801 ttggtcggga tctttgactt cttttccttg gacaagctgt agaaagtcta cagttaaatg
    13861 ttctgcttct tctattgatt ttccgatgac ggcgtctgtc atcatcgagg cacttgccgt
    13921 actgatggag cagccactgc cgtcaaaggc gatatttttg atgattccgt cttttacatc
    13981 gacttgcaat tcgatcacat ctccacatgt ggggttgttc aattcgatct tgcttgtcga
    14041 gtcagtcaag cttccacgat gatgcggatg ggaagaatga tccaagatca cttgacggta
    14101 taaattgtct aatttagata gtcccatgtt ggaaaaactc ctttgttgcc atgattgctt
    14161 caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata aaagctcgcg cgagcagtcg
    14221 ctgatacttc aaggtacttc aatagcggtt gcgcacaatg atgacctgct ctgacggcta
    14281 ccccttccat gtctaaagct gtcgcgacat catggggatg taacccatcc aaattgaatg
    14341 caacgactcc tgtccgaaaa ttcgggtctt gtggaccata aacagtcaat ccatcaattg
    14401 cttgtaattt tggcaacaca taagcggcta gttcttcttc atatcgatgg atattgtcca
    14461 taccgagttt ttgcaaataa tcgattgcat ggcctaatgc gatagcgccg gcgatatttg
    14521 gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt actgtcgtat aattcgacaa
    14581 aatcgatcat ttcgccacca aattcaacag gttccatttc ttctaaccat tttcgtttac
    14641 catataaaac accaatacct gttggtccgc acattttgtg tccgctgaaa gcaaagaaat
    14701 cacagtctag tgcttggaca tcaatggcca tgtgcggggc agattgagca ccatctacta
    14761 cgatgacccc gccgttttca tggacaagtt cagctaattc ttgaacagga ttgaagacac
    14821 ctaagacatt ggatacatga gcaatggaga cgattttggt tttgtccgta atctttttcg
    14881 ctgcatcctc catatccagg aagccttctt ctgttagttc gatatatttc aagacagcac
    14941 ctgttcgttt tgccaactgc tgccacggga taacattaga atgatgttcc atgtaagaga
    15001 gcactatctc gtctcctgct ttgatatagc ggtcgccgaa ggttctggct acccaattca
    15061 agcttgtcgt cgtaccacga gtaaacagca cttctgctgt ttcttttgca tggatgaacg
    15121 cccgtacttt ttctcttgct tgttcataat catgggtcgc gcgttctgct aacgtatgga
    15181 caccgcgatg aacattggcg ttatcgtgtt cgtaatagta cgctaatgcc tggagtactt
    15241 gttttggctt ttgggtcgtc gctgcattat ccagataaac taaaggttca tcattgacgt
    15301 cttgaaataa gataggaaaa tcattgcgta gctttttagc atcgatcatc cattcaactt
    15361 cccttcgatt acttcgacta attctttccg aacttgttct acagggattg ctgttaagac
    15421 agaaccgagg aatcctcgaa tcactaagcg ctctgcttct tctttatgca atcctctact
    15481 catcagatag tacatttctt ctggatcaac tcgtcctaca ctggcagcat gtccagcagt
    15541 tacttcgttt tcatcgatca aaagaatagg attcgcatca ccacgtgctt tatcagaaag
    15601 catcaagaca cgactttctt gttgggcatc tgcacctttt gcccctttca agatgtggcc
    15661 gataccatta aaggtcaatg ttcctttttc tcggatcact ccgtgttgca aaatatgccc
    15721 gatcgtatga ggggctttat tggttactcg tgtatcgatt ccttggattt gtctgcccga
    15781 gctgattgcc acgattttga cttctgcgtg tgcaccttct cctgctaaat ctgagtcaaa
    15841 gtcagcgatc acatgaccgt cattcatgac accaattgcc cagtcgacag aagcatctcg
    15901 taaaatgtgc ccacggcgat tcatataaga agtaatattt tctcctaatt gatctacggc
    15961 tgaatatttg atttttgaac cagctttagc gatgacttcc acgatgatat tacctgatga
    16021 ttttgctact tgttctttgg ttgtctggaa acgttccaga taagaaaatt cactatgttc
    16081 atctgccacg atcaacacat gcttgaagaa gtgctcatcg gatgcgccat cttgaatgaa
    16141 taaggactcg agtggttcat cgatcacgac atttttaggc acatacagga aaataccgct
    16201 gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg ttcatgttta ctgctttttt
    16261 catataatat tcttggacta gttctggata ttcgatcatt gcagtgaaca agtcggtaaa
    16321 aatgactcct ttttctgcta attccacagg cagttgttca aaaatcgtcc atgaaccttg
    16381 ttggacaagc acaggattgt ctttcattgc atcaaatgcc ggaatcgtgc ctgtttcact
    16441 gatggtttgt gtttctttca catcaaataa cgaccagcga tggaatttca cccgatcgat
    16501 atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct tgacggaagg ttctcatcca
    16561 atcaggttcg cctttactta aagaaaaatc atttaccgcg tcaagataat cgagccagtt
    16621 tttttctttc attcttgctc ccccttacgc ttcttcttct ttatagtcga ttcctaattc
    16681 tttactgata ccagcgtagc cttctgcttc cagacgttta gctaggtcag cattccctgt
    16741 catgactact cggccttcca tcatgatgtg aacgacatca ggtgtgatat agttcaataa
    16801 tcgttgatag tgggtgatga tcaatgcacc gaaattgtct ccgcgcattt catttactcc
    16861 tttagcaacg actttcaaag catcgatatc cagacctgaa tcgatttcgt ctaaaattgc
    16921 aaatgtaggt tctaacatca acaattgcag gatctcattc cgtttctttt ctccgccaga
    16981 aaaaccttcg ttcagataac gttcagccat ttcttctggc atatttagta aagccatttt
    17041 ttcatctaac tttttcaaaa atgccataac agaaattttg tcgttttcat cacgtttcgc
    17101 attgatcgct gcacgcatga attccgcatt cgtgatacct ggtatctcac ttggatattg
    17161 catagctaaa aataagccaa gtcgcgcacg ttcatctact tccaagtcta aaacattttt
    17221 cccgtcaaac aaaatttcgc cttctgttac ttcatagttt ggattgccca tgatagcggc
    17281 agataaggta gattttcctg tgccgttagg tcccatgatg gcatggattt ctccggtttt
    17341 cattgttagg tttacgcctt taaggatttc tttttcctcg atcgacacat gtagattttt
    17401 tatttctaag acagacatgc attatacccc caaattctct ttcgttctca ctcattttag
    17461 actaattgag aatgaagcgc aatgaccttc cttactaaaa tcaaatatct tttatttatt
    17521 attaaacaat tttgatttct aaattagaat aggtataaaa aagatgagaa ataagccatt
    17581 acagacttat ttctcatctt ttttgttcca taccttattt tgcatcagcc tttactggtt
    17641 taacagagcc gccccatttg tctgtgatcc attcttggac ttctttgcgg tgaagcactt
    17701 caatcagttt tttgattttg tcactgtctt catcaccttt acgagtcgca ataatattca
    17761 cgtaaggaga gttgtccttt tcaagcaata cggcatcttc taatggattc aaccctgcgc
    17821 cgtaagcgaa gtttgcgttg attgcaacta ggtcaccttc atcattttgg tacgtagaag
    17881 ctaaaagtga tgggtcgatc gtatggttga acttcaagtt cttagggttt tcttcaatat
    17941 catcgaatgt tgctgtttcc agatctactc catctttgac tttgactaag ccagcatctt
    18001 gtagaattgt gatgatgcgt ccccagtctg attcagaatt tgaagtaatg attgttgcac
    18061 catcttttaa ttcggaaaca tctttgactt ttttagagta caagcccatt ggttcgatgt
    18121 ggacagcacc ggcattgaca aagtcatatc ctttttcttt gatttctttg ttcaaataag
    18181 gaatatgctg gaagtaattg gcatcaatgt cgccttcttc caaagattgg ttcggtaata
    18241 catagtcatc gaatttgacg atttcaagat cgattccttc atctttcaat aatggtttca
    18301 catgttccaa aatctctgcg tgcggtgtcg gtgaagcgcc gactttcaat gtcgtgtcgt
    18361 ctgctttatt tgctgaatct ttgctgtcgc tgttgttacc gcatgctgct aatcctacag
    18421 tcagcaaaag cgctgctgca aatccaaaaa ttttcttttt catatcctac atccccctat
    18481 atcctattat cttttgtctg ttttcttagt taagaaatca ccgattcctt gaacgataaa
    18541 tacgattagt aaaataatta aagtcgcaac gaatgtcact gttagattac cacgctggta
    18601 gccttcctgc caagcaagac ctccaagtcc acctgcgccg atcgcgccgg ccatcgcagt
    18661 aaatccgatc atcgagatcg ttgtaactgt taatccagaa atcaatgcgg gaatactttc
    18721 aggtatcaag accttccaga tgatttccca gtaacttgct cccattgcat ctgctgcttc
    18781 cagtacacca gggctgactt cacgaaaagc aatctctact agacgggcat aaaatggtgc
    18841 tgcggataaa acaagagctg gaatggctgc ttgcgcacct agcatagtgc ctacgattgc
    18901 tttagtaaat ggaatgatca aaaccatcag gatcataaat ggcgttgacc ggaaaatatt
    18961 actgatgatc gatacgattc cataaatgat tcgagcatac ggtttgtttt tgcgtccaat
    19021 agagtaaagg atcaatccca aaactaatcc gatgatcgtc actaagacca ttgagattaa
    19081 tgtcatatat agtgtatcaa ggatagcctg attcatcgtt tccgggttga tttgtttaaa
    19141 atctagatat gtttctgaaa agcttttatc cacgatggat cacctcaatt cctactcctt
    19201 gttctttgat gaacacttct gcttgatcta attctttggt ttcaccagtc atcaagacag
    19261 tcaatgaacc aaaggaacct tctttcactt gtttgatctt tccatacaca acgttgacat
    19321 cgatgttata gcgtcgaatc gcttgggaaa tgactggctc gttggcattt ctttcgctaa
    19381 aggtcaaagt tgccaaacgg ccgcttggat tttctttgat gagttcagct aatagttctg
    19441 tcgaatcagc ttctggttcg atgtcttgtt gtacaaagcg tttggttact tcttttttcg
    19501 gttgtctgaa gacagtcaat acgtctcctt cttctacgac tttccccaac tccatcacag
    19561 cgactttgtt gcagattttg cggatcacat tcatttcatg tgtgatcaag acgatggtca
    19621 aattcaattt cttattgatc gctaacaata agtctaatac ttcttctgtt gtttgcgggt
    19681 ccaatgcgct agtagcctca tcacaaagca ggatctcagg atcatttgcc aatgctcgtg
    19741 cgataccgac gcgttgtttc tgtccgccgg aaagttgtga aggatatgct tgtccgcgtc
    19801 cttctaaccc gactaaacgc aacagttctt ctgcccgttc tttacgtttg ctctttggta
    19861 cgccagctaa ttccagcggc agctggatat tttcaagtac agttcttgac cataacaggt
    19921 tgaaatgctg gaagatcata ccgattttct ttcggaatgt acgtaattct ttgtttttta
    19981 attttgtaat atcttgatcg tttacgatca cttcgccgct cgtgggaagc tcaagtccat
    20041 tcaggagacg gactaatgta ctttttcctg cgccagaata gccgacgatt ccataaatat
    20101 cccctttttc aacgtttagt gaaacgtcat tgacggcttg gatgtttccg tttttcccat
    20161 tgaatgtctt tttgacattc gttagttgga ttaatgacat gccattcaca tctttctctt
    20221 atgatttttc aaacaaaaaa gccttcgccc tcttgcctta agcaaaagga cgaaagcttg
    20281 aacttccgtg ttaccacctt gatttaccgt tacttcgcaa taacagcctc ttccagtact
    20341 tggacttagc ttctttccgt atactgtcgc gctgtaacgg gcactcccgt agtaagtttg
    20401 cttacgctcg cttactctgc tctaagacca tcttccatct cattttcgtt acccattttc
    20461 agcaaacagg gctctcttca aacgtccgtg acatgtactc ttcttttcaa agctttctcg
    20521 tttcatatag gattgcttat gattatagca actggaagaa tcagtgtcaa cttttttcaa
    20581 gtgattttct gattttataa ttcgtcaaat tcttttggat caacatcttt gaaacttaag
    20641 atccacgcat tcatttcatc tgaatcattc aagatattga tatcttcacc gattttatca
    20701 ttaacagaag aaactgtacc cgttagcggg ctggcaaatt cattgaccgc tttttccgct
    20761 tcaacttcga tcagaggttc atcttttttt agcgattgtc ctgcttttgg cagattggca
    20821 aacgtgatgt ctccaagttc ttcttgcgct tcatttgtca aaccaacaca gtattctttt
    20881 ccattaaata agatccaaag gttgtccttt ttcttcaaat gcttgcctgc cattttatat
    20941 cagttctcct tcataaattt tttcattgaa tccaattgca acaagctggt cctctcgtaa
    21001 gatcagtggt cgcttgatca gcataccatc tgtcgataag acttggctcg cttttgaagc
    21061 cgtaaactcg cctacttgat ctttcagtcc aagttcccga tatttcatcc cgcttgtgtt
    21121 gaacaggcga cggattggta cttgactcgt ttctagccaa gtctcaatcg ttgaggcctt
    21181 cggtacttct ttgatcatat caacggtttg ataactaacg ccatgctggt ctaaccacgc
    21241 tttggctttt ttgcatgttg aacatttcgg gtaccagtaa aaggtgtaca attttcttac
    21301 ctccttgaag atcttgcagg tgcttcattt tggtatctca tcgataatat caacccgata
    21361 ccgatcatgt tccctaaaat cgaggatcct ccctgactaa tgaaaggcaa tgggatccct
    21421 gttaaaggaa gtaatccgat atttgctcca atattctcaa agacatggaa cagcagcatc
    21481 atgatcaaac cgcttgcgat ataggcgtaa aattcattat tcgtatcgaa acacacccga
    21541 atcatacgat agattaaaat aaaatacaag aaaatgacga atgcaccacc gataaagccg
    21601 aagttttcac cgataacggt aaaaatcata tcggactcac gaacaggcac atagatattg
    21661 cttacgttga atcctttacc aaacaagcca ccggtaccaa ttgccaaaag tccttgggcc
    21721 ggctgataac tcattcctga cgtatcatgg aaagggtcca gccatgaatc aatacgtgca
    21781 aactgataag atttgaatcc aactttataa agcaagtctc ttccgccttc agtcgtaacc
    21841 aagaagatcg ttcctgctcc aacgactact gccaagatta ctacaggtgc gatgatgcgc
    21901 caagagattc cagacatcag gaaaatcccg ccaaagatcg caagaaagac aagcatcgtt
    21961 ccaaagtcat cttgggccat gatcaatgct aaaaccggta ttgtcacgat caagagcttc
    22021 ccaatcagcc aaaagtccga tttcatcgtc cgttctttta cttgcgtatt atgtttagtt
    22081 acgatcagtg ccatcatcaa gatgtacgca atcttcatca actctgctgg ctggaaagta
    22141 aacgcaccga atcgaaacca gtttttagac cctgttgaag cagctaaatt ctgatcatag
    22201 aatttgagca agagtcccat taccactaac cccaaaccat aaaggtaagg agtcagtttc
    22261 cagatccatt tggaattcaa atgcatgatg acaacgattg ctgccgctcc aactaaatac
    22321 cagacaactt gcatccctac gcctttcaag actgctggat tgttcggatc atggctaagt
    22381 gctacatata atgagagcag accaatcaaa cataacagaa agactggtaa aatcacgcca
    22441 taatcaatac gattatcgtt cgaagctttt aatttttttt ccatgtttca tcccttttct
    22501 taaaaaccat actgctgatt attatagcgg tccagttaga taatggaaag tctttccata
    22561 gacatttggg aaaaattaac tttccgtttg tgcagttgct tgagcaaatg cagtacaaaa
    22621 gcctactgct tatctttagt gtatcataga actttttttg atacatgcaa ttatctacca
    22681 gctgagattc tcctcgctgt gagaagtttc tgtgctttca ttgtatcgaa ggatctcttc
    22741 ggcaatctct agcagctgat cagaaaccag attggttcgt tggatgaaat aaaaatatcg
    22801 atggaaattc aacggagtca ttggcacatt ttctggaaca gagcgcttag acaaaatcga
    22861 gcgaccgatt ccttccttca gcaacgcaac aattacatcg ttacttttca cgatcatctt
    22921 cttatcttgg atattcatct cttcaaaata acgctgcgtg taatgaaaaa caccagagtt
    22981 tgcttctctt acaagccaca atttgctttt tgggtcccca gcgatgacta gctggtcttc
    23041 catcaaagag tatctttgta cgccggaagt ctctaatggc ttttcgataa aaccaaaatc
    23101 ggattcatgt ttcgctactt gttctaatga ttcatgagaa ttcgtgattt cgatctcaaa
    23161 atgcacttta ggaaaccttt tgatcagatg tttcatcaac tgaggcaaaa gatagacagc
    23221 aaaggtgtgg gaagcaacga tccgacatac ttcttcttca ttttgttcat tgagcaattt
    23281 ttgctgagtt tcttcccatt cttctaacaa aaccagtgtc cgttcatata ataagtcggc
    23341 ttgcttagtt ggaagcatct cttttcttcc gttacgttga aaaagatcga tattcaattc
    23401 ccgctccaac tgtttgattt gattcgagac tgctggttga gataaaaaca gtacttctgc
    23461 tgctctggaa aaatttcttg tttcatatac tgctcgaaaa gtcgttaata acttaaacat
    23521 ggcaccactt ccataacttt ttatgataat tactataaaa actatttgat ttaaatatac
    23581 aaaaaaccat ctataataga aatgtacaac aaaacagttg ctgatccaac tagtaactta
    23641 gatgaagaca atcagctgtc tgcttgctcg taatcataca ttcctattca ttttacaatg
    23701 tattcgttca tcttggtact aaaatctatt aaataaaaag gagaatatta tatatgaaga
    23761 actttttgaa aattattccg atcccgatct gcgggttgat ccttgggtta gcttcccttg
    23821 gaaatctgtt taaagactat caccatgttg ctttaggcaa tctaactggc ggtatctcta
    23881 tgattcttat gatattcatt tttgttaaat tgtttatctt attcgaacac acgaaacaaa
    23941 cgttgcatga tccaatcatc gcctccgttt caccaacttt tacgatgtca ctcatggtta
    24001 tttgtactta ttttgtttca tatgaaagta ttgcaccaat tgtcaaattc atttggctgt
    24061 gcgctgttat cttccaagtg atccttgtct tttatttcaa ttatcaccat gttatcaaag
    24121 ctgatctttc aattgaagca atttatccaa gctggtttat catttacgtt ggatttgggg
    24181 tcatcactgt aactgccggg aacttcttcc cacttattgg aaaaattttc ttttggattt
    24241 cattgatctg ctatgcgttc ttattgccga tcatcatcaa acgtgtcttt ttcgtaagaa
    24301 atatggctca tccgacacgt ccgctgatta cgatcgttgc agcacctggt tctctttgcc
    24361 taacaggtta tcttaaaaac tttgctgaac ctaattttta tcttgtcgta gcattgttcg
    24421 ctttatctca agtattgtat ttcgttgtgc tgtctatgct tccacgtttg ttcaagcttc
    24481 gtttttatcc aagctatgca gcctttacat tcccgcttgt tatctctgca acagctatat
    24541 ttactactgt tcattacttc aacagtttag gtatctatag tagcgtattg gatttcttaa
    24601 aagttgcgga atgtctgttt gcaagtgtag ttgtcttcta cgttcttgct cattacgtac
    24661 gtttcttaat taaagaacat agcaaacatg gaaaatttgt ttctaaaaaa atcgcttaat
    24721 aatcgattca ataggttgtg aaaaagctgt tctgcatcaa gtaataagaa cggccaaaca
    24781 aaaacagctg ttcatgtttt tgtactggtt gggcttatta ccgcagatgc aagcttttga
    24841 acaccgttta ccaagaggtc gtaaaagaag tggtcagctt caagaaataa gaaggaattt
    24901 acgaaaattg cttctcaaat ttttgtgaaa tttcggctta tttccgtaga agctacttct
    24961 ggaacaccgt ttattcgaaa caagaggtcg tacaagactt ttgtcacgac ctcttgttct
    25021 tttgtcaaaa tactggttta gtgctactat gttcgagtat gcttttagta tttattgacg
    25081 aaaggaaccg atcaaatgaa atcattgtca catccaataa aatctgtaaa ggattcagcg
    25141 gtccgcgaaa tcttacccgg tttactgctt tcttttgcca tcgctgcctt cagcaggctt
    25201 cttgctattt atctgccaca gttaggtgga gcgactattg ctattttatt gggtatttta
    25261 ttaggaaata ctttttttaa acagcctgtt ctagcaatcg gcacaaaatt tgctgaaagc
    25321 aaactattag aattctctgt ttttttacta ggtgcaactg tcacagctca aacgattgct
    25381 caaattggtg taaaaggaat tgtttttgtg ttactgcaaa tgtcagtcac tatcatcggt
    25441 gcttacttga ttggtaaaaa actgcaattc agcgatactt cttctttgat gatggctgga
    25501 ggaaacgctg tttgtggttc ttctgctatt gcttcaatcg ccccttccat ccatgcgaaa
    25561 gaggaagaaa aaggacagat catcacacta gtcaatctac tgggtactgt gatgatgttg
    25621 actctccctt ttcttggatt acagttgttt ggtggaaatc tcattgaaaa aagtgcactt
    25681 ttgggtggta cactccaatc tgttggtcaa gtagtcgctg gggcaagctt acttgacaat
    25741 tctgttgtcg aattcgccat gctatttaag attctacgta ttatgatgct cgttgttgtt
    25801 gtctatctgt ttgaaaaatt catgcacaag aaacaaccag ctctgtttga aacgccaact
    25861 gatacgcaga aaaaacgaaa attaccgctt ccttggtatg tattagggtt tgtgatcttt
    25921 tgtttggtca acagcttgtt ttctttacct gtagttattg accaatcggc acattttatc
    25981 agcagctggt ttgagatcac tgctttagcg gcaattggtc tgcgtttaga ctttgcaaaa
    26041 ttcatgaaag aaggtacacg cttcttatct tatggattag ctgtcgggct tctccaaaca
    26101 gtgatggctt tactatttat ttggctgatc aaagtttaga atgaacatga aaaaacagtt
    26161 gtgattctcg catggaacaa tgtccgaatc acaactgttt ttaattttgc agatatcctt
    26221 ctaacaacaa caattggcgt ttgatttcaa ccccatctat tgcgtttccc atatatcctg
    26281 tcagttttcc attttgcccg atcactcgat gacaaggaat aacgattggc aatggattgg
    26341 ttcgatttgc ctgcccaatc gctcgcacag ctttcggact atcaacagca acggctactt
    26401 ctttatacgt tcggatttct ccataaggaa tggtcgacaa agctttccag acattttgct
    26461 gaaaaggcgt acctctcttt atagataaag gaacatcaaa ttcttttctt tttcccgcaa
    26521 aatattcttc aaactgttct tgtgtgcgcg ttaaaattcg ctgattcgct gattcgcttt
    26581 ctgaaattgg ctgaaaagat acagcagtca atccggattc atctgcatct aaccataaaa
    26641 agccgatagg tgtgtgtatt ttcattttgt ttgctcctag tcaatagact ggaacttcct
    26701 cctttttctc tatccaaaac tacttgccaa ctaataaaaa atgttcttaa aagtttgata
    26761 aaccgaggaa acatggtaaa ctaaatacgt tagtttacaa aaagaatacc tgttctttta
    26821 cgtaaaatca aattttaaag gtgttattta aggtttatta aattttgaat gtaaaccata
    26881 tataaacata cgattgaaag gatagaaacg atgaaagtat caattgttgt cccatgtttt
    26941 aatgaagaaa aaactgttcc tctttttttt gatgaggttg aaaaattcaa aggtgcccat
    27001 gagttcgaat atttgtttat tgacgacgga tcaaaagacg gtacgttagc agcaatcaag
    27061 aagctagcct cagcttatcc ttatgtccgc tttgtctcat tttccagaaa ttttgggaaa
    27121 gaagcggctc tgtacgctgg attgcaagct tctacaggag atttagtgac cgtgatggat
    27181 gttgatctgc aagatccgcc cgaactgctt cctgagatga tcaaccggat cactacttct
    27241 gatatcgatt gtgtcggcac ccggcgttcg actcgtgaag gcgagcctgc gatacgcagt
    27301 ttctttgcga aaaaattcta ccagttgatc aataaaatca gcgatacaga aatggtcgat
    27361 ggcgctcgtg atttccgctt gatgactcgt caaatggtgg atgccgtcct agaattaaca
    27421 gaatacaacc gattttcaaa aggattattt agctgggtcg gatttaaaac agaatatatt
    27481 tcttatgaaa accgcgaacg tgtggctggc gaaacatctt ggtccttctg gaaattattc
    27541 aactactcga ttgatggtat cgtgaacttc tcggatgcac cgttaacgat tgcttcattt
    27601 atcggcgctt tgtcttgtgt cggttctggt attgcaattt tgttcattat tttgcgggca
    27661 ttgttgtttg gtgatcctac atcaggatgg ccttccatgg tctcgatctt cctgttcatt
    27721 ggtggaatcc aattgttatg tcttggaatc atcgggaaat atattgggaa aatcttctta
    27781 gaaacgaaac agcgtcctgt ttatatcata agagaaacag aaaaagtgaa gccaaatgaa
    27841 ttgaagcctc accaataata tacgtcaaat gaagtaaaca tttttttaaa tgaaaaagga
    27901 ctgtgacaaa agtcttgtca cagtcctttt ttccgaataa atggtgttcc agaagtagct
    27961 tcttcggaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc
    28021 ttatttcttg aagctgacca cttctatcac aacctctttc atatcttatt acttcttcat
    28081 tgctgctttt agtgctgctg ctaatgggct ttcttctggt tcttctgctt gtgcatattt
    28141 tttcagcaag cgtcgttctt catgtttggt cattttcttc ttctgctctt ttttatcaag
    28201 caatttttct gtcgttccat cgtatttaca acggaaataa gcaccgtttt taccttcgat
    28261 gatttccatt ttccggtgac attgcgggca tcgatgatta gagacttttg gatcttttct
    28321 tctgcggtag ttgcaatctg aattggtgca gacatagatt ttgccgtcac gtgtgttttt
    28381 ctcgcgcaga ttggccccac attctgggca tttcttttgg gtaatcgaga aatcttgata
    28441 ttcttgtttg cttgatttga tctcattcac caagcgtttt gtttcttttt cgattccatt
    28501 caagaaggtt cctgcttgtt tctttcctgc agcaatagct tcaaggtctt tttcccagct
    28561 ttctgtcaaa tctggtgtta ctaaagaggg atttaccaat actaataatt gcttaccttt
    28621 aggagatact tgcagtcctt gaggggtacg ttccatcagt tcagatttga ttagtttctc
    28681 gataatctct gcacgcgtag ctggggttcc cagactgtat ttttccattt gaccaagcaa
    28741 gcttgcttct gacaatggtt taggcggtgc agatagttct tttttgatgg cgaaatcggg
    28801 attgattctc attccttttt cccatttgac tgtttccgtc tcagtttcag tttcgccttt
    28861 ccatcctgct tggacgacac gattttgccg aaaaatgaat cgttctttac caaattcaac
    28921 cgtaactttt gtttggctga ctttatgggg ttgagcaaac agtcctaaaa aacgagagac
    28981 aatcatttga tagatttttc gctcttcatt acttaatttt tcataacgtg ctctgttttc
    29041 agttggcaaa agtgcatggt gatcggtcac tttactgtct tggaatactt ttgtctgttg
    29101 gacgatggct ccgtttttca aatactgctt gacctcagga gcaaagtcgc tgactgcttg
    29161 caatcgttct ttcatcgtac ttttcatgtc atttgtcaga tatttgctgt ctgtacgcgg
    29221 ataagtaacg atcttatggg tctcatacaa actttgtaca agacttaatg ttttctttgc
    29281 agaatagcca tagcgttgat tggcttctct ttggatctct gttaaatcat aaggcaatgg
    29341 cgcagattcc gtttttaatt tttcttggac ttctgtgaca gttcctttgc ttgtactgat
    29401 caattgaaca agtttttgtg cttcttcttg tgtttttaaa gcaaattgat ttttttgaag
    29461 cattttggct tgttcttttc cagcttgcaa cgtgatcgtg aaatacgttt gtggacggaa
    29521 ctgctcgatt ttcttttctt ggtctctcac taatgctagt gtcggtgtct gcacacggcc
    29581 tgctgataaa ttatccgcgt acttcacagt taaggctctg gtcacattca atcctaccag
    29641 ccagtccgct tttgcccggg caagagcaga ttcatacaat gcatcatatt ctttcgcagg
    29701 tcttagttgc ttgaatccag ttttgattgc tttatctgtt tgagaagaga tccataaccg
    29761 cttcactggt ttattgaaat gcacatactc taagatccag cgagcaacaa gttccccttc
    29821 tcttccggca tctgtcgcaa tgactgcttc tgagacatct tttcgctgag ccagctgctt
    29881 gattgctttt aattgatgtc ctgttttagg aagtggtttg attcctagtt ttttagggat
    29941 cattggcaac gtgtccattt gccagctctg ccattcctta ttcagatctt ccggcatttt
    30001 caatcctaat aaatgaccaa gggcccaagt aacgatcacc ttgggtcctt cataataatt
    30061 tttgtgcttt tgattagcac caagcacctt gcttaggtct ttcgcaacgc ttggtttttc
    30121 cgctagaatt agttgtttca taaaattcct ttctgctttc tactgtgatg ggctgatgat
    30181 ttttcaacaa cgcaagtccc ttgtattgct gtaaatcatc gtcacactct tcacaccagc
    30241 gctcatctcc gtcattccaa aaggcgctga gaaagttact ttttgcattg atatattcat
    30301 aattttgatg gatttcgttc cattgttttt cgtacgcttg tcgtgcttct tcgaaagact
    30361 cgaatgtttc ttcttcttca atatcttctt cccagtcttc aaaaaaccac caaggctcat
    30421 tgtccccgaa catggtaatt acttgataca tactcgtgct ccttctttca gcttttctag
    30481 actcatctta gtcacttttc aagaaagttg caagaatttt ctcttataaa agggtcattt
    30541 aatgaacatc ttaaaattgt tacaaatgaa tctgattgtt taaggcaaag tagcgataaa
    30601 gttattgatc atccaaaaat agctgtcttc tggatcgatt gttggttctt ttgtaaagta
    30661 tcctaaaaaa cgtaaggaaa gataaccgtg gatcatacta cgaagttccc tgcttcgatg
    30721 aactttttct tctttcgtta attgaaaagc ctcaaatatc tgcaaaatga tctgattcgt
    30781 ttcatggatt ccatctaata aaacagcttc attggtatgc ggtacactga tcagtaactc
    30841 atacactgct tgatttgaaa aagcaaagga tcgataagtt tctgcataga tctgcagtgc
    30901 ttcctgacca ctttttccca caagagcccg tcgcagctgg tcattaagtt cctgcagaag
    30961 tttcgctgtt agcgctgttt tgacatcacg aatatttttg aaatggttat acagcgaggg
    31021 agccttgata tccaagcgtt tggctaaatg ctgaaaggtt atttgctgaa catccatctc
    31081 ttctgctagt tctcgaaagc agtcgatgat tttctcttga gacagatttc tttttttcat
    31141 ttccatgttt cctctcataa aataaatcct tcactctttc agtttaccac gaaaaagaaa
    31201 gagaaaaaag agactttcat tgataaacta catattgtag tttataataa agagctgtag
    31261 atgaaaattt tcagaaagaa ggagtctaat tggctgcaag aataaaggaa aaattattta
    31321 ggaaaagaga aacttgggaa caaaacttgt ttgtcttatg gtttggaaca tttatggctg
    31381 gtatcggatt tagtttagtc atgcctttta tgtcacttta catagatacg ttaggtaatt
    31441 atagtacgtc acaattgaac ttttggagcg gattgacttt ttcatctact ttcttggtga
    31501 ctacgcttat ctctccttgg tggggaaaat tagccgacca aaaaggaaga aaattgatgc
    31561 tgttgcgtgc ttcattagga atggcaatcg tcatttcttt gatgggttgc gtgactagtg
    31621 tgtatcaatt agtcgcattg cgtctgcttc aagggatttt ttctggttat atcagtaatg
    31681 ccactgccct tgtcgcaact gggacgccaa aagaaaaaag tggtcaagtt cttggaacct
    31741 tagcaactgg ttctgttaca ggaacattac ttggaccgct tcttggcggg atctctgctt
    31801 cagcgtttgg ctatcgccct acctttttca ttacagggat catcttattt ttcgtcttct
    31861 tgttgagtct gtttttcgtc catgaaaaat ttgtcccagt gaaaaaagaa aacatggttt
    31921 ctgccaaaca gattttcaaa gacttgaaat atccacacgt cgtcatcggt atgttcgtca
    31981 caacgatgat catccaagca tccaacaatt ccattagtcc aatcatcagt ctgtacattc
    32041 gccaattgct gcatggacat gggaatgtta cgttgatcag cggaatcatt gcttcgatac
    32101 ctgggattgc cactttgata gccgctccaa gatttggtcg cttaggtgac aagattggga
    32161 gtgaaagaat acttgcgatt ggtttgggtt ttgcgattct ggtttatatc ccaatggcat
    32221 tcgtcacgaa tgtttggcaa ttagctgcct tacgtttttt gattggtatc tctgatgctt
    32281 gtctgctccc tgctgtccaa gcattgatca caaaatattc gcctcatcaa gctgctggac
    32341 ggatcttcag ttataaccaa tctttccaag caactggtaa cgtagtaggg cctatgatcg
    32401 gctccagtgt ttccagtgca tttggttatc gtggcgtctt tctttcgact tctcttcttg
    32461 ttttggtcaa cttcttgctt gtacgacaca atacacaaga gatccatcag caagaaactg
    32521 cagaagaaaa agcttcagga gtctccatag cttcagggca ctctccttct catagacaat
    32581 aagcgtaaat gaaaaacact gcttgaacag ttgtcacaga ctgtccttgc agtgtttttg
    32641 tttaggcctg ttttttcaag aactcagata agaggaataa tcccttttcc aaggtttctt
    32701 ttttacaaca gtaacctagt cgggcatgct tcggtaaatc aaaggcactt cctggtacta
    32761 atagtactcc tgtttcttct aataaattga tacaaaatgt ctggtcatct tcttcgatgg
    32821 ctaatttaat gaaagaagta gagacgtaat tgggagcgac aagttcaact ttcggttcat
    32881 tggctaccca ttgttttaaa atcacaaggt tctctgtaat gatctttttg tttcgcgcca
    32941 atattttttc tttattcttc aatgcatgta cagcaagatc atctgacagg actccgccgc
    33001 aaatcatcgt gtagtctcga tactttcgaa aaacttctgc caactcaggt ccagtagctg
    33061 tccagccaat acgaatacct ggaatggagt aggttttgga tagggaattg gttgctatac
    33121 ctttttcata gacatctacg atcgacaaga attcaccttt atccgttaaa ggcgcatata
    33181 cttcatctat caatacatat gcatctactg tacgagcaat ttctgcgatt ttttctaaag
    33241 ttttccgatc tagcaatgtg cctgtaggat tgtttgcact attcaaacag atgatttttg
    33301 tttcaggttt gactaaggct tctagctgtt ctaaatcaaa ctgccaatct tcttcttcct
    33361 tcaaatgaac aaagtcaaca gcggcaccta aagatttggg gatgtcatat aattgctgat
    33421 aagtaggaag catcgaaaca acatggtctc ctgcttccac taacgcgtat agagctaaaa
    33481 gattagcacc agtcgctcca ttcgtttgta agatgttttc cggatcgacg gtttgataaa
    33541 gagaagcgac ttcttgtttg aaaacatctg atccttcgat ccagccatag tccaatggtt
    33601 cgtttttgcg tttttcgaaa aatttactta cgctagtccc atctaagcca acgagttcgt
    33661 ctaatgtcaa tgcttcgatc gagctttgtg caatatcata taccgctttc ttttcccatt
    33721 tatttagcca ttcttctaca ccgaaatttg cgattttcat gtttctttcc cccttatatc
    33781 ttgatccagc ctacagcaag caacccgatt gaaagcagtg cggccacaca aatcagccca
    33841 atcattactt tttcttttcc cgtaatttga tgtttggttt cacgacaagc gatccagtag
    33901 aagcaaaaac caggaagata ggcaatacta cataaaagga cataagaaaa tcctgctaaa
    33961 atgatgccca tcaattcaaa aactacagca atcccaccga tcactacttg ttttttgatt
    34021 ccttctttta aagaaaactg gacttgatag atcccgacaa gtagataaca aatcaaaatt
    34081 gccgctgtac ataaagaata ggcaaattga taggcataat cagtaactaa gaaagtcaat
    34141 aagaatagat tagtcaagat tgcagtaaca actaacgaat aagttggggc atttttttta
    34201 ttcgtttttc cccaaccttt cggcaacaga ccgtctcgag ccatcaacat cgttgtttct
    34261 gctgggagca tcgtccaaga caaccaagaa ccaatgatcg aaataatcaa gccgatattg
    34321 atcagcgctg ctccccaatt tcccacgatt ccttttaaaa tatttgccat agcaggttgt
    34381 gaaatagttg ctaactcttc ttgagataag acgccgtatg gcaagatcga agcaaggata
    34441 tagataaaca ataaacaaag cagaccaatg atcgatgctt gctgggcttc tgaacgtttc
    34501 ttcgcgcggt tggctaaaac acttgcgcct tcgatcccta caaagaccca catcataacc
    34561 atcaaacaac ctttaatctg caaccagatc gttccttgct gccctgttcc attcactaga
    34621 ttatcagata cgttacccca aaaatcagca gtgaatatat tcactttaaa tgcgataaat
    34681 acaaatatta aaaataggaa taatggcact aatttacaga tcgtcacgat tgtattgatg
    34741 acgctcgctc cttcaactcc attgtttact aataaagtca gaccccaaat aaaaatactt
    34801 gcaaataaaa tagaaggaac attttgtcca cctttgaata tcgggaaaaa ttctcccatg
    34861 gcgctcatca gcatcgttgc aaaagcaaca tttcccagcc acgcagacaa ccagtaaccc
    34921 caaccactga tgaagccacc taatttacca aatgaagctt ccgcgtaacc gaaaatacca
    34981 ccatctagat ctggacgctt ttctcctaaa tgattcagcg ataataccaa agctaaaata
    35041 ccaatcccaa cgattatcca tgaaaggatc gctggccctg gagctgcaga actagccaga
    35101 tcacttgtga tcccaaacac accgcttccg attgacgaac tgacaaccag agcaaccagt
    35161 ccccatttgc taatacccgt cgatttcttt tccatttcca tcaccctcac ttataaaatt
    35221 gaacaaatca cttgcaaaag attcccttct attttgcaac tgctcttgct taacaaaaag
    35281 caactttatt catattattg gatattatga actttttaca caagtatttc aagtataaaa
    35341 tgaataaaaa ttaattttat tgcaaaaaaa cagctatgct atccaaaaga aaaggtgttt
    35401 cttttgttag ccatagctgt tttgattcat ttaatatcgc ttaaacgtat tgtaaaactt
    35461 ttgtttcttg atatgcgtgg tcgatcaaac caccacccag acattccatt ccatcataaa
    35521 atacaactgc ttgtccagga gtgattgcac gtacaggttc gtcgaatacg acttctgcac
    35581 gattatcatc caataagcga actgtcacag gaacgtcttg ctgacgataa cggaatttcg
    35641 ctgtacactt gaattctttt ggcattggtt cattcgtcgt aaagtggatt tcacttgcat
    35701 ccaagcttgt ggcatacaat gcaggatgat ggaacccttg ccccacatat aatgtgtttg
    35761 tcgctagatc ttttcctaca acaaaccagg gttcttgcga atctcctccg ccaccgatcc
    35821 ctagtccttg acgttgacca atcgtataat acatcaagcc agcatgttgt cctttgactt
    35881 caccatcaag agtgaccata tttccttttt ttgctggcag atagttgctc aaaaattgtt
    35941 tgaagttctt ttcaccaata aagcagattc ccgttgaatc ttttttctta gcagtcgcaa
    36001 ggcctgcgcg ttcagcaatt gcccgtactt ctgatttttc catgccgcct agagggaaca
    36061 tcgtttttga aagctgttct tgtgataatt ggctaaggaa gtaagtttga tctttattat
    36121 tgtctactcc acgaagcatg tggaccacgc cgttttcatc gcgagtgact tgcgcataat
    36181 gtccagtagc tacataatcg gcgccaagat ccattgcata atccaagaat gctttgaatt
    36241 tgatttcttt gttgcacatc acgtctgggt taggtgtacg tcctgcgcgg tattcggcta
    36301 ggaaatattc aaatacacga tcccaatatt ctttttcaaa attcacagag taataaggaa
    36361 tcccgatttg agcagctact ttcgctacat ccttataatc ttccgtggcc gtgcatacac
    36421 cgttttcgtc cgtgtcatcc caatttttca tgaatattcc gatcacgtcg tatccttgtt
    36481 cctttaacaa aagtgctgtt acagatgagt cgacaccgcc gctcatccct acgacaacgc
    36541 gtgttttgct gttgtctgtc atgatatcac catcatttca attagattct gaaacatcta
    36601 cgagttgaag gtcgcaactt ttcagtattt tacattatac gtcatatttc tgaaaatttc
    36661 atcccttaat tatcaagtcg taaaaatact gttctactgg acctattcag tacacaaaca
    36721 tgatagtcaa ccaaaaaatc actcatcttt tgagcattcg atataaaaaa ttggcatact
    36781 cataaaaagg gccagccttt cggcaataag gaataatttt ataaattgtg aaaaactatt
    36841 tttcaaaatt gcttcttatc gcttaaagct gcacgtctca tttacaacct tttcttttta
    36901 gatgttatga gttgctcaga taagtgattt tgtttactct aagaaaaaag ggaggcaaaa
    36961 gatacatgaa ttactttatc agtgatatgc actttttcca tgaacgctta ttaggaaaaa
    37021 acgactttgc tccgcggctc tttgcaaatg tggatgaaat gaaccaacag ttgatcacta
    37081 gctggaatgc agtaataaaa gaaaccgacc atgtttatca tctaggcgac cttgctatgc
    37141 atccgcaata tgaaaaagca aatgcagaaa ttttggaact ggtcactcaa ctgcaaggaa
    37201 caatccactt gatcaaaggg aaccatgaca gtcgcgcttt tttccgttat ttagaaaaaa
    37261 atgatcctag gcttactgac ggaagtccca aattttattt ttatgatgta ggtgccatcg
    37321 ttaaattcga ccatcaacaa tattacttga ctcattatcc gatgttgctc gggcctaacg
    37381 acactatccg caatcttcat ggacatatcc atcattacag cgtacctatc gagaacgatg
    37441 tcaatgtagg cgtagacgca ccggaaagag aactcttaaa ggaaaaacta ccatttggca
    37501 ctccgttatc tgagcaacaa atcaaagaaa tctatgaagc aaagaggaag tacgataggt
    37561 cgtgaaaaaa ctgccctata tcaagtatta aacaaataaa gataattact catatttttc
    37621 atttgagtag gcttattgcc acaggaacat taggatcaga aatggagttg taacaagact
    37681 tctgtcacaa ctccattcca tactattcgt ctttttgctc atggtgttct tttaccagtt
    37741 ttttctgtaa ttctttatca taatttcggg cagctgacgt taaaaataaa acgattccgc
    37801 ataataaaat cccgattccg gaaaacaaga acattttttc tacccctaaa cgatcagcta
    37861 atggtcctgc aaataataat ccaattggac ctgtcaggtt catcaaagag ttgaagttcc
    37921 ctaagacacg acctaaaaca ttcgattcat aactctgctg gatcattgcc atcagcagtg
    37981 tattgaaata tggcgttgca caacctgctc ctgcattcaa gataagaaag tacaagaacc
    38041 cttgactatc gtttggaacc aatccgcttg caccgatcgt caggcccata atgatgtagg
    38101 ctataataat aggtttcatt cggtctttcc atgttcctat aaaactgatc agtgcaccgc
    38161 ctaagagcat tccgactgcg tagactactt ccactgctcc cgcctgtcca acggaaccac
    38221 caaagtaatc tagcgtcatc aatggataca gactgattgc tggcatgaac aacagcatca
    38281 caaatgcacc attgatcgtt atgtaccata accctttatt atccatcaat tgctgcagcc
    38341 caaattttgc atctttaaaa atagtaagcg gaacttccaa tatttcagga gaaacttttg
    38401 ggatcttcac aaaaatcaat aagccgacac caaaaactgc ccccaataca tctaataaaa
    38461 taaggtaatt gacaggaacg actgaaaaca aagccgcacc tagagctggc gcaatgatga
    38521 aattcgctga ttggaccatt cctaactgtc cattcgtcct tgttatgtga gaagatggaa
    38581 cgattgtcgg cataatcgac tggatcgttg gcatttggaa tgtttgtgcg accgaacgca
    38641 taaacaaaga cacaaaaact agccaaatcg ggaaggattc tgaaatcgtt ccgactactg
    38701 ctaaaataag cgcgaaaatc gctacaataa tatccgtgac aatcaaaaga gcttttttat
    38761 cccagcgatc cactagcggt cctacaaatg ggcttagtaa aaccatggga atcattccta
    38821 gtaaagtagc aaaactcaaa attgtcgccg aaccggtttc tcttgtcaaa taccagatga
    38881 tcgcatattg gacgaccata ctagtgattc ctgacaaaaa ctgtcccgat aaaaagaaca
    38941 aataatttct cttccaattc gttaaaacga ccgactgttc attttccata aacatccctc
    39001 ttttcttttt cattttgtat agcgtttgca caacaatcat tatacgggat tcttttttca
    39061 aaaaatacct ttttccctaa ataacaatcg tttcaattta aatttcttta cttagaaaca
    39121 ataaatcagc aaaatattaa aaataacaac atgtgttaaa tttaaaaata aaaaagacaa
    39181 ctaatttgcg tcgtccttac aaagttagtc atctaacaat aggccgtttc aaaactagtt
    39241 gtctttttat atccacagat cattcgactt tttcgaataa cccacggcta atcatcccat
    39301 ccatcagaaa ataaaattta tcttggctca ttttctgttt tggatctttg aaaatattga
    39361 ctgctttcaa tccatttgct gtagtgatcg acattttcaa tgtttgaata tcttttgata
    39421 cagtgatttt taatttgaat ccttctttgc tttgcggcgt ggcatctaat ggtcggatca
    39481 attgataatt gcctccgttt gtttctgtaa atccattatc gcgcaatgta tatctttttg
    39541 catcaggatg caaacgatag ctaattggtg cgcccagtac cgttgctgtt gtttcaaatg
    39601 ccatgatttt cacctcgata aaattagttt ctctttatta taaaagttta tcctgatttt
    39661 tactaggttt ttcagttaag ttccctaaaa aatgaaggcg ttctctcaga catctgttca
    39721 tataagaatg acttcactag ttattttttg acagcacgaa ctaattgatc aaggaaatac
    39781 tggatgtcgt cttcggtatt tcccaaacca aagctgatac ggatggattc ttttagtatc
    39841 ggtgtatctt ctccatacat tgcttccaat acgtgtgaag gctcgatatt tcctgctgta
    39901 caagctgaac cagtagaaat agcgacccca ttaagatcaa gcttcatcag tagcaaatca
    39961 tttgcttcgc caattaagcg aagattcaac acatgaggga gtttattcgt taaatcacca
    40021 tttacttgat aagggatttc gtttgcttct aatgtatcaa gaatcaattg ctggaatcct
    40081 ttatatttgt tgttacgtgc ttcacgttct tcaggtgaca gcagttcaac tgcttttgcc
    40141 ataccccaga tccctgctaa attttctgta cctgcacgcc gcttttcttc ctgctccccg
    40201 cctaaaagca atgcagggat atttactccg tctcgtttat ataagaaacc gacgcctttt
    40261 ggcccattga ttttatgagc agagatactt aataaatcga ttcctaattc ttctggatgg
    40321 atcgtttggt ttccgtacgc ttgtaccgca tctgtatgga aataagctgg atgatccgct
    40381 aaaagttcac cgatttcttt gattggcatc aaatttccga tttcattgtt cccgtacatc
    40441 acagagacca agatcgtatc ttctcgtaac gcttttttga cctcttcgat cgacagattc
    40501 cctttttcat ctacaggcag atatgtaacg tcaaacccct ttgtctctaa atatttcatg
    40561 gtattaagaa cagcaggatg ttcgatagct gtagtgatta gatgtctgcc ttctttttga
    40621 cgcgagaacg ctgtttctaa gatagctgta ttatcccctt ctgttccccc actgttaaag
    40681 atgatctcgt gaggttttgc ttgcagactg tctgcaatga tttgtcttgc tgtttccaat
    40741 ttttcatgcg cgtggcgacc aaatccatgt atgcttgaag gatttccaaa cgtgtcttgc
    40801 atgattgccg tcatcgcttc aattacttgt ggatgcatgg gggtagttgc cgcatgatcc
    40861 agataaatag ttttcacttc attcatccct tccaaattat tgctaggcac attctagcat
    40921 aaaatagcga acaaaccaaa gaagagctca tttttgtcta gaggacgttt catgggcata
    40981 gaaaaaagga gctgtgacaa aagtcttgtc gcagctcctt gaagctgctc acttctgtca
    41041 caacttctt
//
LOCUS       DAJSBC010000021        40714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSBC010000021 DAJSBC010000000
VERSION     DAJSBC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.021"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(14..406)
                     /gene="rpsI"
                     /locus_tag="ONT65_001115"
     CDS             complement(14..406)
                     /gene="rpsI"
                     /locus_tag="ONT65_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2328762.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(422..865)
                     /gene="rplM"
                     /locus_tag="ONT65_001116"
     CDS             complement(422..865)
                     /gene="rplM"
                     /locus_tag="ONT65_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR2328763.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(1384..1851)
                     /locus_tag="ONT65_001117"
     CDS             complement(1384..1851)
                     /locus_tag="ONT65_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR2328764.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(1971..3509)
                     /locus_tag="ONT65_001118"
     CDS             complement(1971..3509)
                     /locus_tag="ONT65_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR2328765.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            3589..4275
                     /locus_tag="ONT65_001119"
     CDS             3589..4275
                     /locus_tag="ONT65_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR2328766.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(4476..5003)
                     /gene="msrA"
                     /locus_tag="ONT65_001120"
     CDS             complement(4476..5003)
                     /gene="msrA"
                     /locus_tag="ONT65_001120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2328767.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5087..5764)
                     /gene="pgmB"
                     /locus_tag="ONT65_001121"
     CDS             complement(5087..5764)
                     /gene="pgmB"
                     /locus_tag="ONT65_001121"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2328768.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(5761..6891)
                     /locus_tag="ONT65_001122"
     CDS             complement(5761..6891)
                     /locus_tag="ONT65_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2328769.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(6891..9074)
                     /locus_tag="ONT65_001123"
     CDS             complement(6891..9074)
                     /locus_tag="ONT65_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2328770.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9071..10096)
                     /locus_tag="ONT65_001124"
     CDS             complement(9071..10096)
                     /locus_tag="ONT65_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2328771.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10141..10968)
                     /locus_tag="ONT65_001125"
     CDS             complement(10141..10968)
                     /locus_tag="ONT65_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2328772.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(10965..12032)
                     /locus_tag="ONT65_001126"
     CDS             complement(10965..12032)
                     /locus_tag="ONT65_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR2328773.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12058..12894)
                     /locus_tag="ONT65_001127"
     CDS             complement(12058..12894)
                     /locus_tag="ONT65_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2328774.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(12891..13760)
                     /locus_tag="ONT65_001128"
     CDS             complement(12891..13760)
                     /locus_tag="ONT65_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2328775.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(13830..15113)
                     /locus_tag="ONT65_001129"
     CDS             complement(13830..15113)
                     /locus_tag="ONT65_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2328776.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15127..16812)
                     /locus_tag="ONT65_001130"
     CDS             complement(15127..16812)
                     /locus_tag="ONT65_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR2328777.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17087..18112
                     /locus_tag="ONT65_001131"
     CDS             17087..18112
                     /locus_tag="ONT65_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2328778.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(18707..22381)
                     /gene="rpoC"
                     /locus_tag="ONT65_001132"
     CDS             complement(18707..22381)
                     /gene="rpoC"
                     /locus_tag="ONT65_001132"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR2328779.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(22439..26056)
                     /gene="rpoB"
                     /locus_tag="ONT65_001133"
     CDS             complement(22439..26056)
                     /gene="rpoB"
                     /locus_tag="ONT65_001133"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2328780.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            26784..27752
                     /locus_tag="ONT65_001134"
     CDS             26784..27752
                     /locus_tag="ONT65_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2328781.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            27770..28675
                     /locus_tag="ONT65_001135"
     CDS             27770..28675
                     /locus_tag="ONT65_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328782.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            28672..29478
                     /locus_tag="ONT65_001136"
     CDS             28672..29478
                     /locus_tag="ONT65_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328783.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            29614..30603
                     /locus_tag="ONT65_001137"
     CDS             29614..30603
                     /locus_tag="ONT65_001137"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR2328784.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(30920..31444)
                     /locus_tag="ONT65_001138"
     CDS             complement(30920..31444)
                     /locus_tag="ONT65_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR2328785.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            31731..33542
                     /gene="scm"
                     /locus_tag="ONT65_001139"
     CDS             31731..33542
                     /gene="scm"
                     /locus_tag="ONT65_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR2328786.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(33697..34683)
                     /locus_tag="ONT65_001140"
     CDS             complement(33697..34683)
                     /locus_tag="ONT65_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2328787.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(34706..35272)
                     /locus_tag="ONT65_001141"
     CDS             complement(34706..35272)
                     /locus_tag="ONT65_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR2328788.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(35286..35702)
                     /locus_tag="ONT65_001142"
     CDS             complement(35286..35702)
                     /locus_tag="ONT65_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2328789.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGNYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(36159..38093)
                     /locus_tag="ONT65_001143"
     CDS             complement(36159..38093)
                     /locus_tag="ONT65_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2328790.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            38374..39330
                     /locus_tag="ONT65_001144"
     CDS             38374..39330
                     /locus_tag="ONT65_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR2328791.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            39420..40523
                     /locus_tag="ONT65_001145"
     CDS             39420..40523
                     /locus_tag="ONT65_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2328792.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            40536..>40714
                     /locus_tag="ONT65_001146"
     CDS             40536..>40714
                     /locus_tag="ONT65_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2328793.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT        11697 a         8716 c         7696 g        12605 t
ORIGIN      
        1 tatataaagg atattaacgt tttgagaatt gtgaagcttt acgtgctttc ttaagacctg
       61 gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta
      121 aacggaagtc aggatcaact tctagcaatg cacgagcgat accatgacgg atagctcctg
      181 attgtccagc gtatcctccg ccgtttacgt taacaaatac gtcatatgcg ccttttgttt
      241 cagtaacagc gaaaggttga ttgattactt cacgcaagtc agcatgtgga atgtattctt
      301 caacatcttt tttatttaca gtaattttgc cagttcctgg tactagacgt acgcgagcaa
      361 ctgcgttttt acgacggcct gtgccgatat attgaacttg tgccaatgaa attccctcct
      421 attagattaa gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt ggatgttctg
      481 ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca
      541 taccttttac agaagtttcg atcaaacgac gagaattttt agcacgcaat tcgccagcag
      601 tgattgattt caatcctcct gggtagttgc tgtgacggta ataaattttg tcagtcgctt
      661 ttttacctgt taatttcact ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca
      721 aatgaggtgt gaaagttggt ttatttttac cacgtagtac agatgcaaca actgttgaaa
      781 gacgtcccaa tggaacatca gttgcgtcta ctacatacca tttacgttct acttcgcctg
      841 gtttggccat atatgttgta cgcacgttat tttcctccaa tttaagttct gatgtttgac
      901 atacacttcg agtttccggg gctctttgtg gggcaaacaa taccatttaa catattaccc
      961 gctaatctag tcaatgtcaa cgtttttctg cttgattgaa aaatttatat gaaactttac
     1021 ttcttttctt tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg
     1081 ctgtgacaaa agtcttgtca caacccctta ttttgaataa acggtgttct cctgccaact
     1141 cctcggtcac aagtcacaat atccataaaa cataagaagc tgttttttaa aattgtttct
     1201 tgtgactcgg agctgaacgg caggcttaca acctcttggt aaacggtgtt caatcagctg
     1261 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct
     1321 ctaatactca ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag
     1381 tttttacgcg tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt
     1441 ttctgtggct gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc
     1501 agaaacttcg attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt
     1561 ttccaaacgt tctggaatag tacgatccca gcggccaatt tcatcaggga tttttgtgtt
     1621 atctgcaaat tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga
     1681 tacttcatct aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt
     1741 aaggaaacct ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct
     1801 tagatctgca actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc
     1861 ctcctaacat tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa
     1921 tcatttacct gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca
     1981 gaatcagcta attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt
     2041 tgtgtcgaaa caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact
     2101 ccaacttgct ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg
     2161 atgcttctcc agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa
     2221 acaagcgggt aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac
     2281 ccgcctgtag cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga
     2341 taaaggttga gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc
     2401 cgctgtaaac cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca
     2461 agtaaatagg gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa
     2521 agcagtgcat catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa
     2581 aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc
     2641 caatgtttct gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga
     2701 gtagtaataa aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca
     2761 aaagcaccta accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata
     2821 tcatttggca aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg
     2881 gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct
     2941 gcccattgga attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg
     3001 atcagttcgc caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt
     3061 tttttgaata attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt
     3121 ggatgaatcg gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt
     3181 ttttctgctt cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt
     3241 ttctcttcat ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg
     3301 attgtgtctg aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag
     3361 atcaattcct gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa
     3421 taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt
     3481 ccgatatcta atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa
     3541 aatttagttt aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat
     3601 tttatcattg gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta
     3661 ggtcactcta ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg
     3721 tatgaattga ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg
     3781 tgtcggttgc ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg
     3841 ttctttactt tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg
     3901 ctattttcgt tgatggattt attttacttc atggtccatt ctcacacgct attctgttct
     3961 tgggcaattt tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg
     4021 ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc
     4081 gatactctgt tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc
     4141 ttgtttcttg ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa
     4201 aaaaagttgg aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt
     4261 catcttaggt attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt
     4321 gttctcttac caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta
     4381 aatggtgttc aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg
     4441 atatacggtg ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga
     4501 cgaaggaaac ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc
     4561 ttgtaataat cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt
     4621 gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca
     4681 atatttcgtt cttcttcatc attatagtag atcactggac gataagaatc accgcgatca
     4741 gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga
     4801 tcataagaaa taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg
     4861 cctgttgtta cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc
     4921 acagaaatga tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct
     4981 gcgaaaactg ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc
     5041 caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata
     5101 cggcttctga taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca
     5161 catattcagc agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag
     5221 ccaaagctgc ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta
     5281 cattcaattg ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt
     5341 gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc
     5401 gtgcattttt gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa
     5461 gaacagctat tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat
     5521 atagattgtt tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca
     5581 tttttccaaa tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct
     5641 cgttttcttt ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt
     5701 aatgataacg agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt
     5761 tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt
     5821 gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag
     5881 gatcggaaaa acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact
     5941 tccgcaaata gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc
     6001 ttttcctttt agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg
     6061 ctgtacccgt ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt
     6121 ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc
     6181 ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc
     6241 taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc
     6301 gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg
     6361 cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt
     6421 ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc
     6481 gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg
     6541 tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg
     6601 gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc
     6661 gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa
     6721 ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag
     6781 atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc
     6841 taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc
     6901 ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt
     6961 aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac
     7021 ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga
     7081 gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc
     7141 catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct
     7201 aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg
     7261 caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca
     7321 tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac
     7381 agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa
     7441 tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc
     7501 ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat
     7561 aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta
     7621 acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat
     7681 tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca
     7741 gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa
     7801 aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg
     7861 atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat
     7921 gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca
     7981 ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac
     8041 agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt
     8101 tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca
     8161 ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc
     8221 gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct
     8281 gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg
     8341 gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc
     8401 tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga
     8461 ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta
     8521 ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt
     8581 tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct
     8641 acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta
     8701 ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg
     8761 agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact
     8821 ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct
     8881 gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca
     8941 aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat
     9001 ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt
     9061 tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca
     9121 ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga
     9181 gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct
     9241 tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag
     9301 cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa
     9361 acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat
     9421 cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac
     9481 caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca
     9541 tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt
     9601 tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt
     9661 aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac
     9721 gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc
     9781 ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga
     9841 gggcagccaa tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg
     9901 gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc
     9961 ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat
    10021 ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga
    10081 ttgctacttt cttcatttat ttctctctcc tttgcttatt tacttattgt tattctttct
    10141 ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg
    10201 caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat
    10261 ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc
    10321 agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat actctttttg
    10381 cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat
    10441 gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt
    10501 gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc
    10561 ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct
    10621 tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac
    10681 gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc
    10741 attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt
    10801 cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa
    10861 caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc
    10921 taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct
    10981 cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa
    11041 gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa
    11101 ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa
    11161 tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct
    11221 tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc
    11281 ccgccatagc cgatccctat taaagaagct tgtaatgcgg cagcactgcc actcgtttca
    11341 ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct
    11401 ttcataggat ctagcgtgta atcggcaccg tgtttttctg ctaattctct acgctgcgct
    11461 atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt
    11521 tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc
    11581 cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc
    11641 aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga
    11701 atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt
    11761 tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt
    11821 tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata
    11881 aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt
    11941 actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct
    12001 attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta
    12061 tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat
    12121 aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc
    12181 tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag
    12241 gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga
    12301 aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag
    12361 accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag
    12421 catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt
    12481 atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga
    12541 aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt
    12601 tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga
    12661 gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc
    12721 ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc
    12781 ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa
    12841 tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag
    12901 tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa tagaatcgat
    12961 atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct
    13021 aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata
    13081 gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg
    13141 aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact
    13201 gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac
    13261 aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa
    13321 agctgcaacc tatctactaa cagatcattg acgattccat aactattatt gaacatatat
    13381 ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc
    13441 accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct
    13501 gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat
    13561 tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta
    13621 ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact
    13681 aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa
    13741 tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt
    13801 ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa
    13861 gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct
    13921 gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt
    13981 acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca
    14041 ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga
    14101 gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca
    14161 gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca
    14221 tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct
    14281 tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg
    14341 aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt
    14401 tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca
    14461 tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt
    14521 ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga
    14581 gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt
    14641 tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt
    14701 ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc
    14761 tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca
    14821 aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt
    14881 tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct
    14941 ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag
    15001 tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca
    15061 gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc
    15121 cacctcttat tttattattt tcagccacat atattcatac ggctggaata tcaactttcg
    15181 ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa
    15241 aagataacct gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc
    15301 ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg
    15361 atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc
    15421 tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg
    15481 attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag
    15541 cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc
    15601 taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata
    15661 cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg
    15721 ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc
    15781 tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt
    15841 tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg
    15901 aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc
    15961 gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac
    16021 cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc
    16081 tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg
    16141 aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac
    16201 ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt
    16261 ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac
    16321 gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa
    16381 gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa
    16441 cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt
    16501 gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa
    16561 gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg
    16621 caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc
    16681 ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg
    16741 ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag
    16801 ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat
    16861 tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata
    16921 caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat
    16981 gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac
    17041 tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat
    17101 ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa
    17161 ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa
    17221 ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc
    17281 attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca
    17341 atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac
    17401 accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat
    17461 tattatttta agctctacga tggatgaaac acttcttttt gaattggatc aatataatat
    17521 tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac
    17581 ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca
    17641 tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta
    17701 ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg
    17761 ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc
    17821 aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca
    17881 atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc
    17941 ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa
    18001 agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat
    18061 tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat
    18121 tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt caatcactca
    18181 tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga
    18241 tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact
    18301 atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt
    18361 atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat
    18421 gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa
    18481 taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt
    18541 tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat
    18601 gttgttaaga tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa
    18661 aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat
    18721 tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt
    18781 cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag
    18841 ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac
    18901 cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga
    18961 aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag
    19021 gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata
    19081 atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt
    19141 gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac
    19201 gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt
    19261 gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg
    19321 ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga
    19381 ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta
    19441 cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag
    19501 gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca
    19561 actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt
    19621 caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg
    19681 tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt
    19741 ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat
    19801 gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt
    19861 catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac
    19921 ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag
    19981 tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca
    20041 agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag
    20101 gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag
    20161 ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt
    20221 gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat
    20281 ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt
    20341 caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg
    20401 catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg
    20461 ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg
    20521 gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt
    20581 atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt
    20641 caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt
    20701 tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat
    20761 gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca
    20821 ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat
    20881 cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta
    20941 acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt
    21001 ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc
    21061 gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc
    21121 ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt
    21181 cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa
    21241 gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt
    21301 ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt
    21361 tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt
    21421 tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg
    21481 catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca
    21541 agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca
    21601 agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa
    21661 taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt
    21721 ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt
    21781 cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg
    21841 cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt
    21901 cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat
    21961 atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca
    22021 tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat
    22081 gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa
    22141 cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag
    22201 gaccaaaaat ccgctcacag aacaaccctt cacgttcagg ttttaaggta cgatagttaa
    22261 tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca
    22321 aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca
    22381 tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt
    22441 agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca
    22501 cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca
    22561 aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg
    22621 tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata
    22681 cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt
    22741 atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat
    22801 aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa
    22861 attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg
    22921 catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca
    22981 cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg
    23041 ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg
    23101 gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt
    23161 gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc
    23221 catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc
    23281 cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac
    23341 gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca
    23401 catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac
    23461 gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc
    23521 cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga
    23581 tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg
    23641 tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga
    23701 tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt
    23761 tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt
    23821 gttccataga tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa
    23881 cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt
    23941 atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa
    24001 cgacaccgtc atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca
    24061 taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt
    24121 tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg
    24181 caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt
    24241 ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat
    24301 tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt
    24361 cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa
    24421 atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc
    24481 cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt
    24541 cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt
    24601 gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa
    24661 agaattcttt gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg
    24721 tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga
    24781 tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt
    24841 cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa
    24901 tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt
    24961 ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat
    25021 ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga
    25081 aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct
    25141 cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca
    25201 aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat
    25261 cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac
    25321 gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat
    25381 gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg
    25441 tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac
    25501 ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc caagctccac
    25561 ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga
    25621 aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga
    25681 tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga
    25741 tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg
    25801 catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca taatctacaa
    25861 attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc
    25921 taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta
    25981 attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta
    26041 ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc
    26101 aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct
    26161 tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct
    26221 acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca
    26281 tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc aacacttcct
    26341 ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt
    26401 atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt
    26461 tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac
    26521 ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg
    26581 acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa
    26641 aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc
    26701 aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc
    26761 ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc
    26821 tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac
    26881 aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag
    26941 gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt
    27001 atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag
    27061 aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa
    27121 caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca
    27181 catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa
    27241 aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata
    27301 acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag
    27361 cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga
    27421 aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg
    27481 cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag
    27541 ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca
    27601 aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg
    27661 tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca
    27721 tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt
    27781 gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg
    27841 tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc
    27901 tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg
    27961 tcttctaggc tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa
    28021 attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac
    28081 atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg
    28141 ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc
    28201 tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg
    28261 tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa
    28321 gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca
    28381 aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc
    28441 tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt
    28501 cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga
    28561 accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca
    28621 gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac
    28681 gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat
    28741 gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga
    28801 aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag
    28861 ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa
    28921 tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa
    28981 gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa
    29041 ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat
    29101 cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg
    29161 atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac
    29221 cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc
    29281 actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc
    29341 atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca
    29401 gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa
    29461 gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc
    29521 ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac
    29581 tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca
    29641 acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag
    29701 ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa
    29761 aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta
    29821 ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa
    29881 acgatgttga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat
    29941 tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg
    30001 ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa
    30061 cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt
    30121 gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga
    30181 aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt
    30241 catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat
    30301 ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag
    30361 ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg
    30421 gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga
    30481 acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca
    30541 aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa
    30601 taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc
    30661 tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt
    30721 attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca
    30781 agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta
    30841 tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag
    30901 ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct
    30961 gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg
    31021 gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt
    31081 aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag
    31141 cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg
    31201 tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat
    31261 cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga
    31321 tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa
    31381 acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat
    31441 ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct aggataccta
    31501 cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt
    31561 tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga
    31621 acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag
    31681 gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa
    31741 gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg
    31801 tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta
    31861 actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg
    31921 tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg
    31981 gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt
    32041 ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg
    32101 gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg
    32161 atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg
    32221 agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta
    32281 aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt
    32341 tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc
    32401 cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt
    32461 ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag
    32521 atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca
    32581 gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc
    32641 aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc
    32701 ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag
    32761 atacatctac cacaagtgat agctcagatt catcaacttc aagcgacagt acagatacta
    32821 cctctaccac aagtgatagc acggatacat cagcttcaag cgacagtaca gatactacct
    32881 ctaccacaag tgatagcacg gacacatcag cttcaagtga cagtacagat actacctcta
    32941 ccacaagtga cagcacggac acgtcagctt caagcgacag tacagatact acctctacca
    33001 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    33061 gtgatagcac ggacacgtca gcttcaagcg acagtacgga tacatctacc acaacaagtg
    33121 atagcacgga cacgtcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    33181 gctcagattc atctaccact acaagtgata gcacggacac gtcagcttca agtgacagta
    33241 cagatacatc ctctaccaca agtgatagct cagattcatc aacttcaagc gatagtacag
    33301 acacgtcgtc taccacaagc gatacacgtc atgcaagtgt ctccttctca aaaacaagtt
    33361 cggataacag gacgaacttt aacttaacta ataaaacaag caatgacaat ggaaacaaat
    33421 caaaaagagc acttcctaaa actggaagtc aatcgaataa ttggataaca ctcgcaggcg
    33481 ttatattact tgtaatcggt ttaagaatca cttttatgag ttataagaaa tcgaaaaggt
    33541 aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg gacgttgctc tttatttttg
    33601 ctttttatca taaatacaag aactacaatt caatttgtca aaaaacacgt agatttcctt
    33661 ctgagaaatt tatttacgta tcgaaccttt ccctattcag tctttgcgat ccaaaatcag
    33721 ttgggtaagg gaaaagagat tctcttttgt tcgaagagag gtggcaggta atttctttat
    33781 ttccttcaaa gccttttctg tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc
    33841 tgtatgttca acgattttgc gtacggcgtc gacatcttct tctgtcattg ccgctttctt
    33901 ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa gagtagatca acggtaaaga
    33961 atagacccct tgacgcatgt cttctagtac aggtttgccg atttcctggc tctcttttgt
    34021 ataatctaaa atatcatcga taatctgaaa ggccatcccg atagaaagtc cgatatctcc
    34081 tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa gcgccaacag aacaacttaa
    34141 ttggaacagt tcagctgtct tacctgaaat attttccaaa tattctttta cagaaagttc
    34201 ataattatag cgatcatcca tctgccctaa ttcgccattc aatacttttt ccatactgcg
    34261 agaattcaat tggagacttt tcaaggaagt ggagtaatca gacaatagtt taaaacagca
    34321 gacaaataaa tagtctcctg catagacaga aacgacatta ccaaactgac tacggatatt
    34381 aggctgacca cgtcgcagat ccgcttcatc aacgatatca tcatggatca gtgtggccgt
    34441 atggagcatt tcgatagaag ctgccaaagc gatggctttg tctcgatctt gctctggtcc
    34501 aaattgtgag aacaatagct gataggcagg acgcaacagc tttccgccgg catggatcgt
    34561 ttctttgatg gcttgttcga caggtttgtt tttcaggcgg acagtttctt ccatccgttt
    34621 tagtgtttct gtcaattctt tacttaaagc aggatagttt tcccacattg gatgtagctt
    34681 cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa cattgggctc catttttgtt
    34741 tggttccctg ctcataatct ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat
    34801 aattcgctgc gatcccgatt gcaatccctg ataaaatccc taaaactgaa aggactggca
    34861 agtacaacat gactgtccat gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat
    34921 tatgcatgaa accgccggcc gcactaattc cgatgatact gacacgtttt ggtcctaatt
    34981 gcttgaccaa taacattcca aaataactaa gcaaagcacc actcatacta taaaggaatg
    35041 tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg tagccaaagt agtaagaagc
    35101 tgtcttgttt tttcatggta aaaatggcaa cgatcgtgat tagattggct aagcctagtt
    35161 ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt ttccaataaa ccaatgatca
    35221 ctccttgtgc aacaagtagc gaaatataaa tcattttttg taatttactc atggtgtgtg
    35281 aaggtctaat aaatcagact gtcttcgtca cctccatctg ttgaacggac ttcgatcact
    35341 aatttatgtg gcaaacagac gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg
    35401 cagatctggt catcgcaatc tgcttctttt atccgtactc gatcgccatc tatctcgatc
    35461 aagttataat ttccgtgttc atctttgtaa gtatatgtgt tgctctcttc tccagccatc
    35521 aaagggaatg ttttgatttc tgtgccatcc acacgcagta ccgcttcttt ttctgctgtt
    35581 gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa aagaaagaac agtcaaaagc
    35641 aaaataataa aaacatccca aggtttgagc agactttttt cagcaaattc tttaaggctc
    35701 atttttgttc tcctttttcc agattaactc acccaatttt agcataaaaa caagaggttg
    35761 tgacacctgt gtcttgcttt gagtaataag aacgaagaat agaaaacagc tttccatgtt
    35821 ttctaattct ttgggcttat taccgagaag cagcaggtgg aacaccgttt ataacaagag
    35881 gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa tttacgaaaa ttacttttca
    35941 agtttttgtg aaatttcggc ttatttttcg aagaagctac ttctggaaca ccgtttataa
    36001 caagaggttg taacagaggt tgtgacacct gtgtcttgct catggaggct tatctcgggc
    36061 atcttattcc tttaatttta cataaaaagg aggctgtgac ggtttgtctc agcccctttc
    36121 tattgaaatc ttgcattcaa ataacgttct ttatctattt atttcttgcc gtactgggaa
    36181 cttggtctac cgtaccacca attacctagt gtacgttgta gccatggaat tacccagcga
    36241 tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga aagcaacaaa aatgaaccaa
    36301 atgtttaccc agtaaaacat gcctgataag cagaacgtga tcaccaatac aatcgttgtc
    36361 gcactgcata gccatgtgaa taatccagca atcaatgcta atgccaaggc aatttcaaag
    36421 aataccatga atttttggaa gaacattgct acatcattgt ttggcatgat cacttgcatg
    36481 atctttgtga accagtctgg tgacttctca atcacagcca ttggttcttc accgtatgca
    36541 taactcaaac caaaatgtgc agcttgagtc gctgcttctc cggcacttgc ccctgctccg
    36601 ctggcagctg tggttgcatc accagcagca gaagcaccac tggcaacatc tgttacattg
    36661 tattcccatg tccaagggaa cacactgttt ccagtgaacc atgatccttc accaaaccaa
    36721 gtacttggtt tgaacacttc accattccca atgatttttt tggaagcttc aacgaaccaa
    36781 actaaaccat agaaaatacg aagtggcaca ctccataaga cattgccgta acgagaagaa
    36841 tgtcctcgag tcacgttgcg atcatctttg atatggaaaa actcatgcat taaatattgg
    36901 aacatataat aaccagaacg aatatcaaag aagtatttca aattcacgat atgtttcatt
    36961 acgatagcta aaaatccgct taaatggatc ttatcaaata ggttagctac cccccatttg
    37021 gcaccaatag aaaccataaa tccttgatag tttcctttaa atttatgctt ttctccgcct
    37081 ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg tttgttcagc agcttgaacg
    37141 atctgcggtg tcggtgttcc tgggaattct tcataaaaga ctaaatcacc aatgatatag
    37201 atgtttttat cttcgtagcc ttttgcctgc atatattcat tggcgatcaa ccgctgtccg
    37261 cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc ctttgacacc agctgtccaa
    37321 atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt tgatatgatc agcagccact
    37381 tctacgattg gtgcattcaa taataattcc acgttgtgct tacgcaaata gcgttcagct
    37441 tttgcagcat catttcgatc caacatattc aagatcgtcg gcattgcctc tacgaccatc
    37501 agtgtgattt cgtttggatc taatttataa tctttggcta ggcgattttt ccagtcgatc
    37561 agttccccaa tcatttcaat accggtaaat ccagaaccac agacaacgaa agtcagcatg
    37621 gctttacgca gttcatcatc tggttccaat gcagcttttg ctactgtctt ctcaatatgt
    37681 tcccgaatcc ttacagaatc ttcaaatgac caaagggtga atccgtgtgc tttgacacca
    37741 ggtgtaccaa agtcattcgg ctctccgccc attccaagga tcagctgatc aaaaggatag
    37801 gaacctaatt tggtcttgac gatttttcgt tctttatcga taccgattac agtgtctgtg
    37861 acaagttgta cattcttttg acgagcaaac aaatgctgca gatcgtattg gattgcttct
    37921 ggttctacac gtccgccagc tacttcatgc aactcggtca tcatagtatg gtacgaatga
    37981 cgatcgatca gagtaattgc tacatcttct tttttcagtt tctttgccaa gtattttgta
    38041 gctgaaacac cggcatatcc tgctcctaca acgacaatat tttgctttgc cattgatcat
    38101 ccgctcctta tagactatct atataatgaa tatatgataa ttcttttagt ttgatactta
    38161 ttacacaatc tccatatatt atatagatgt tctgttcttt tgtctaactc cttttggatt
    38221 tttttcaaat tttaaaataa agcgtttttt cacgaaattt gctgaaagaa aacgtttgaa
    38281 atattacatg ggttatttat caaaaatagt tgacttatag gctaaaaaga ttagaatgta
    38341 taatgaatca caaagaaaaa ttgtggacta gaaatggaat ggtggggtat taaaatgaag
    38401 aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt cagctctagt gttaggcgct
    38461 tgcggcgcag atgacaacgg aagctcaaac tcttcttctg aatcatctac agcacaatct
    38521 tcgactgcaa aaagcacaac agaatcatct gcaaaagttg tagctggcgg agatttgcaa
    38581 gacggcacat ataaattaga agaaaaaaat tattcaaatg gttaccgtgc agtctttgaa
    38641 atggttgtaa aagacggcaa aatcactgaa tcaaaatatg ataacgtcaa tgaaaacggc
    38701 gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg aagcaaaatc tggcacaaac
    38761 ccagaaaaat acattcctga gttgaatgac caattcttaa aagcacaaag cgcaagcggt
    38821 gtggaagtcg taactggagc tactcactca tctgaaagct tccaaaacta cgcacaacaa
    38881 ttgatccaag ctgctcaagc tggtaacaca gatacaatcg aaatcgacaa tggtgctgat
    38941 ttgaaagacg gcacttacaa gcttgaagaa aagaactact ctaacggcta ccgtgtgcaa
    39001 ttcgaaatga cagttgctgg cggcaaagta actgaatcaa actttgatta tatcgacaaa
    39061 gacggtaaat caaaacaaga tgatacagaa tataatgaaa atatgaaagc aaaaagcggt
    39121 acagaaccaa aaacttacat ccctactctg aacgacgaat tcgtcaaagc aatgggcgaa
    39181 gaagatggct cacctgctaa tgtagaagtt gttacaggtg ctacacacag ctcacattca
    39241 ttcatcatgt acgcacaaca attagtgaat gcagctgaaa aaggcgacac tcaaacaatc
    39301 gaagtagata atatcgtaac agaaaaataa tcacataatc atactaataa aagttggagg
    39361 gtgggacgat agccagatta ggcagtcgtc tcacctttca aaatgaagag aggaaaaaga
    39421 tgcggaaaaa actactattt tccttaatca cactgggcat ctcttttatg gtgctaactg
    39481 gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc agcagctacc aagatattga
    39541 aagatccata ttctgatgaa cagttcttgt tagggactta cgttcgtatc cgtgtgtatg
    39601 atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg agtaaaagaa cttggtgata
    39661 aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt gaatgagcaa gctgggatca
    39721 aaccagtaaa agtttctgat gacgtctaca cgttagtcaa gcgggcctac gaatatagcc
    39781 aagattctca aggcggattc gatatggcga ttggtgcaat cacacaatta tggcgaatcg
    39841 gttttgatga cgccagaaaa ccttcccaag aagaaatcga tcaggcattg aaattagtgg
    39901 attatcataa gatcgaacta aatgacaaag aaaaaactgt ttatctaaaa gaaaaaggaa
    39961 tgatcatcga tttaggtgca atcgccaaag gttacattac ggacgaagta gtgaaagtct
    40021 tgaaaaaaca aggagtgacc actgccatcg ttgacttggg cggtaatgtc tatgtcttag
    40081 gccacagccc aagaggcgaa aaccaagact ggacagtcgg catccaagat ccgaacatgg
    40141 ctcgcggatc tgttttaggt tctattaaag aacggaacaa aacattagtt acctctggaa
    40201 tttacgagcg ttatttagaa gtagatggca aaaaatacca tcatttgttt gatccaaaaa
    40261 cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat cataacagat aaatcaattg
    40321 atggtgacgg gttgtctaca gcagtctttt caatgggtgt gaaaaaagga ctggaatacg
    40381 tcgaaagcga attgaataac gggacggaag cgatcttcgt gacaaaagac gataaagtat
    40441 atgtgaccga tccaatcaaa gacaccttca aattatcgga ggactcccat tacacgatgg
    40501 gtgaccgttc tgaactaaaa taaggagtac gaaccatgac actaaaaatc tttttagaag
    40561 tagttgaaat acgaacaaag gtggctagtg tatttccttt tgcggtaggg gtattattct
    40621 ctatcgcttt ttttcatgag atacattggc taaacacttt tttatttttt attggaatgc
    40681 tagtttttga tttagcgact accgctatca ataa
//
LOCUS       DAJSBC010000022        39126 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSBC010000022 DAJSBC010000000
VERSION     DAJSBC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.022"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..290)
                     /locus_tag="ONT65_001147"
     CDS             complement(<1..290)
                     /locus_tag="ONT65_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2328794.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="ONT65_001148"
     CDS             complement(283..624)
                     /locus_tag="ONT65_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328795.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="ONT65_001149"
     CDS             complement(798..1523)
                     /locus_tag="ONT65_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2328796.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="ONT65_001150"
     CDS             1576..2052
                     /locus_tag="ONT65_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328797.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="ONT65_001151"
     CDS             complement(2058..2240)
                     /locus_tag="ONT65_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328798.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="ONT65_001152"
     CDS             complement(2240..2539)
                     /locus_tag="ONT65_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328799.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="ONT65_001153"
     CDS             complement(2644..2847)
                     /locus_tag="ONT65_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328800.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="ONT65_001154"
     CDS             complement(2862..3608)
                     /locus_tag="ONT65_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR2328801.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="ONT65_001155"
     CDS             3686..3994
                     /locus_tag="ONT65_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328802.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="ONT65_001156"
     CDS             complement(3965..4222)
                     /locus_tag="ONT65_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR2328803.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="ONT65_001157"
     CDS             complement(4520..4660)
                     /locus_tag="ONT65_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328804.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            4960..5280
                     /locus_tag="ONT65_001158"
     CDS             4960..5280
                     /locus_tag="ONT65_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328805.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5298..5729
                     /locus_tag="ONT65_001159"
     CDS             5298..5729
                     /locus_tag="ONT65_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2328806.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5856..6440
                     /locus_tag="ONT65_001160"
     CDS             5856..6440
                     /locus_tag="ONT65_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2328807.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6542..7690
                     /locus_tag="ONT65_001161"
     CDS             6542..7690
                     /locus_tag="ONT65_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2328808.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(7779..9404)
                     /gene="groL"
                     /locus_tag="ONT65_001162"
     CDS             complement(7779..9404)
                     /gene="groL"
                     /locus_tag="ONT65_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR2328809.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9455..9739)
                     /gene="groES"
                     /locus_tag="ONT65_001163"
     CDS             complement(9455..9739)
                     /gene="groES"
                     /locus_tag="ONT65_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR2328810.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9964..10626
                     /locus_tag="ONT65_001164"
     CDS             9964..10626
                     /locus_tag="ONT65_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2328811.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(10702..11994)
                     /locus_tag="ONT65_001165"
     CDS             complement(10702..11994)
                     /locus_tag="ONT65_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2328812.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12164..12793
                     /locus_tag="ONT65_001166"
     CDS             12164..12793
                     /locus_tag="ONT65_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR2328813.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(12896..13705)
                     /locus_tag="ONT65_001167"
     CDS             complement(12896..13705)
                     /locus_tag="ONT65_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2328814.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13760..14629)
                     /locus_tag="ONT65_001168"
     CDS             complement(13760..14629)
                     /locus_tag="ONT65_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR2328815.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(14630..15946)
                     /locus_tag="ONT65_001169"
     CDS             complement(14630..15946)
                     /locus_tag="ONT65_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328816.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(15943..17778)
                     /gene="walK"
                     /locus_tag="ONT65_001170"
     CDS             complement(15943..17778)
                     /gene="walK"
                     /locus_tag="ONT65_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR2328817.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(17783..18487)
                     /locus_tag="ONT65_001171"
     CDS             complement(17783..18487)
                     /locus_tag="ONT65_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2328818.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(18677..19537)
                     /locus_tag="ONT65_001172"
     CDS             complement(18677..19537)
                     /locus_tag="ONT65_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2328819.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(19524..20093)
                     /locus_tag="ONT65_001173"
     CDS             complement(19524..20093)
                     /locus_tag="ONT65_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR2328820.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            complement(20667..21836)
                     /locus_tag="ONT65_001174"
     CDS             complement(20667..21836)
                     /locus_tag="ONT65_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2328821.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            complement(21880..22110)
                     /locus_tag="ONT65_001175"
     CDS             complement(21880..22110)
                     /locus_tag="ONT65_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328822.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            22186..22258
                     /locus_tag="ONT65_001176"
     tRNA            22186..22258
                     /locus_tag="ONT65_001176"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22219..22221,aa:Lys,seq:ctt)
     gene            22420..22641
                     /locus_tag="ONT65_001177"
     CDS             22420..22641
                     /locus_tag="ONT65_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328823.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(22965..23210)
                     /locus_tag="ONT65_001178"
     CDS             complement(22965..23210)
                     /locus_tag="ONT65_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328824.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(23204..23605)
                     /locus_tag="ONT65_001179"
     CDS             complement(23204..23605)
                     /locus_tag="ONT65_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2328825.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="ONT65_001180"
     CDS             complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="ONT65_001180"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR2328826.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(25613..27379)
                     /locus_tag="ONT65_001181"
     CDS             complement(25613..27379)
                     /locus_tag="ONT65_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR2328827.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(27392..28186)
                     /locus_tag="ONT65_001182"
     CDS             complement(27392..28186)
                     /locus_tag="ONT65_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2328828.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(28197..29573)
                     /locus_tag="ONT65_001183"
     CDS             complement(28197..29573)
                     /locus_tag="ONT65_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2328829.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(29702..30349)
                     /locus_tag="ONT65_001184"
     CDS             complement(29702..30349)
                     /locus_tag="ONT65_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2328830.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="ONT65_001185"
     CDS             complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="ONT65_001185"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2328831.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="ONT65_001186"
     CDS             complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="ONT65_001186"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2328832.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(33016..34647)
                     /locus_tag="ONT65_001187"
     CDS             complement(33016..34647)
                     /locus_tag="ONT65_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2328833.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            34964..35635
                     /locus_tag="ONT65_001188"
     CDS             34964..35635
                     /locus_tag="ONT65_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2328834.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(36471..37427)
                     /locus_tag="ONT65_001189"
     CDS             complement(36471..37427)
                     /locus_tag="ONT65_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328835.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(37536..37781)
                     /locus_tag="ONT65_001190"
                     /pseudo
     CDS             complement(37536..37781)
                     /locus_tag="ONT65_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37926..38348)
                     /locus_tag="ONT65_001191"
     CDS             complement(37926..38348)
                     /locus_tag="ONT65_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328836.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(38528..38899)
                     /locus_tag="ONT65_001192"
     CDS             complement(38528..38899)
                     /locus_tag="ONT65_001192"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2328837.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT        11869 a         7801 c         6016 g        13440 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttgt taaacttgtt
     4861 tacaaaacat agtatagatt aataatgtat ataagtcaac tatttttaca ttatttatct
     4921 aaactttttt tgtaagcatt cagaaaggtt gatttatcaa tgaatactta cgaaataata
     4981 aaggagttag caaaagaaaa aggattatcc attagacagc tcgaaatgaa tttcgggtat
     5041 tcgaatggat atttaggaag ttggaaaaga caaactccta attctattga gttagctcgg
     5101 ttagctgatt actttggagt ctctgtagac taccttctag gtagagaaga aagagaaacc
     5161 cctaaacatg tggatttatc agaaaacgat actgtttttt cttttgatgg aaaagaaata
     5221 tctaaggaga caatgcgtaa agcgattgca attgctaaag ctttagagga aaatgaatag
     5281 ttggagtgat gggttgtatg tatttgaaat tgaaagaaat gttgagcgaa tacaatttga
     5341 aagtagttta catggaaatg aaagagcctg gcttctatta tccgaagcct agaatcattt
     5401 ttttgaatga aaatctatat ggcgaaactg cagaagcctt tcatttatct catgaacttg
     5461 cgcatttcac cgcttctcat tttgaatatt cagttttgta cgatacttct acaacttttc
     5521 actcaaaatt tgaaacagaa gcagataaaa tagctattct aattttatta aatatctata
     5581 ttgagaacga actgactgat gaatctcagt ttaatctcga aaaatttatg gagtattatt
     5641 ctattcagaa taagctcaga tacacttgtt atgctgtctg ccaatgctat tttaagaaaa
     5701 aatattctta cgcaaggcaa tatgtatgaa tacatcaaga attattaaaa aaaccgattt
     5761 cagcaaaaca ttaaaaaagc ccgtgctggc acacggactc atacctcatt tctgagatcg
     5821 caaatatatt ataacaagaa gtgaggaata tttaaatggc aaaaaaagtt atgggtcaag
     5881 acggaaaaat gtataaagtt agtaaacctt tttacaaaaa agtatggttt tgggtattag
     5941 cagtaatttt aattataatt attggttctg ctttaaatgg tggatcagat agtaataaag
     6001 caagtgataa tggtggcgaa aaagtaacta aatcttcaac ctctgcttca tcttctaaag
     6061 aagaaaaaag cgataccttc tataagattg gtgacactgt aaaagttggt gatgctgaat
     6121 atacactaaa tagtgtagaa ctaactgatg aaagaaatca atttgaagaa aaccaacctg
     6181 cacaagtagt aaaaattact tataccgtaa aaaatgatgg cgattcagat atccctgtag
     6241 gtacagatgt ggaagtatat ggaccagatg acaaaaaatc agagacatat gccaacgaaa
     6301 atacaatggg atctgttgct ccaggaaaac aaatggatgt aactgctcat ttcacgttaa
     6361 accaaaaggg agaaatagaa atccacttct ctcctttagt atcatttgaa aaagcagcta
     6421 tttttaaagc aactgtataa taaataaaaa acacgcccca ccgtccagag taagagcgtg
     6481 cttttagaaa acaaacctat ataggtctat ttgttatgcc tattatagca aatgatagga
     6541 gatgttaaaa gtgtgggttg aacagaccaa agatggccaa ttcaagttta ttgaaagata
     6601 tgtagatcct tatacagaaa aaacacgaaa aaaatctaca acacttacaa gtaattcacc
     6661 acaagcgtgg aaaaaagctc agaaaattct agataaaaaa attaaagaag cactcgaaga
     6721 ttacaataaa tcagatatca cttttggtga gctgtataaa gaatggtatg aatattataa
     6781 gcagcatgtt aaacgtacta gctatttgaa ggttccaatg atgatgaaac atgtttctaa
     6841 gcacataagc gatgatacga tcgttagaaa cattgatgag acactcatta ataagataat
     6901 tgaagatatg tatacgtttg gtgacctctc actgaactac acaaaacaaa caaaaacaac
     6961 tctatctgtt atgctaaact atgcaatcga tagaaaatac attcaaagaa accctgcgct
     7021 agcagttaaa atccatccta aaaaagtgga agaagaaaaa agaaggcttt ctatgaataa
     7081 aaaatatctg gagaaagaag aaattgatca aatactgaaa cagctatact ccaatcctcg
     7141 cagaaaacta cacggcataa tagctgagtt tctatattta actggtttga gatatgggga
     7201 attgctagct ttgcaaatga aggactatga ggatgggaaa atttccatta atgggacctt
     7261 agattacaca tctgtgaaaa tggataatgc tataaaaaca actccaaaaa atacttattc
     7321 gcaacgtgaa gtgcaattac ccaatcgtgc aaaagaattg attgaaagtg tgatagctga
     7381 caatattctt gcaggtagac ccacagatcc cgatcaatat atatttatat ctacaagtgg
     7441 cactccgctt gcgctgcact catttaacgc tatactccat aaagtagagg aagagttgga
     7501 attagaaaaa agtctatcct cacatatatt cagacatagc cacgtttcac tattatctga
     7561 attaggcgta cctcttaaag ccattatgga gcgtgtaggg cattctgatg caaacacaac
     7621 tctgtctatt tataaccatg taacaaaaag agccaaacaa caagtaattg ataaactaaa
     7681 tagcctttga tatttttgcc ccttctttgc cccttttgct taaaataaaa caaaaaaaga
     7741 agaaagaacc ctgttaaaat agggttcttt cttaattact acatcatacc gcccatcatt
     7801 gatggatcca ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct
     7861 tctgtcgtta ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact
     7921 ttagttgggt ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg
     7981 aaaccagtgc ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca
     8041 gcgttttcag cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct
     8101 gttgccacgt ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct
     8161 gtaccaccac ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct
     8221 tcaatgcgta attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca
     8281 gctacaccac ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa
     8341 gttgtttcag caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct
     8401 ccagaacctt ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct
     8461 aagttttcga ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca
     8521 cctgtcaaaa tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct
     8581 tttacagcta ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct
     8641 tcaccatcta catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct
     8701 aacaatggca gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg
     8761 ttttctagaa cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca
     8821 cgatcaaact gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg
     8881 attgtgatga caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact
     8941 ttatcagaac ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca
     9001 gttgagatat tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga
     9061 atgcctagag gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt
     9121 gtcagaacag ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact
     9181 tctgatacaa gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg
     9241 atcgtcacac catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt
     9301 cggcctttag gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac
     9361 atagctgcgc gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat
     9421 tgattatttt tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt
     9481 ttcctgcgac gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt
     9541 cgaacataac ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca
     9601 aacggccttc accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag
     9661 ctaaaacgat tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat
     9721 cacctaatgg ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac
     9781 tcttaacctt cgagtgctaa cctacatttt taataatact cattctccct cttttttgca
     9841 agcttttaaa tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat
     9901 aattgaaatc aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat
     9961 tacatgtctt taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc
    10021 tcacccttga tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca
    10081 actgcagtca tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt
    10141 gaacccttag cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat
    10201 ggattggttg gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct
    10261 gtattgtttg gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg
    10321 gaagcacccg cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta
    10381 ttccgccgca gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg
    10441 atcagctctt tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc
    10501 ctaggtttct tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc
    10561 acacacgtag gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta
    10621 gtataaacat gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag
    10681 cgacaggagt atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt
    10741 ctgtctttcc tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa
    10801 aaaactaatt gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa
    10861 acccgagagg atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa
    10921 tccaaaatat aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg
    10981 aacagccatc atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt
    11041 gaaaaaggct ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa
    11101 aaccgtcata aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg
    11161 atcgagaaat tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga
    11221 ggctaagatt tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt
    11281 gaccatcgca gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa
    11341 aagcacacca aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac
    11401 tccttgaaca acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa
    11461 agtgactata acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat
    11521 cttctctctt tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat
    11581 gatgagccca gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat
    11641 ctgagtattt agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg
    11701 taagcgatcg atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa
    11761 ggctatcaca tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt
    11821 gtctatccac tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc
    11881 aacaataatt attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag
    11941 attcacaaca gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta
    12001 tctccttcaa cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca
    12061 cgaaccatga aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc
    12121 agcaactgct tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat
    12181 tggagctact ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga
    12241 cgtgacggca atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa
    12301 ggatttattt gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt
    12361 caacgctccc aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat
    12421 gcttgaagga accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga
    12481 tacagaaaag acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac
    12541 agctttcaat atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac
    12601 ttatatcagt ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt
    12661 aagtgatgat tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta
    12721 cgcgattgca ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt
    12781 agtcagtcga taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa
    12841 accaaccctg attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa
    12901 tcgaaaatag ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa
    12961 cgccaaaatc tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat
    13021 tgttttcctt gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag
    13081 tcatgacaag tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt
    13141 ttaaattcca cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca
    13201 aataaccatc ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg
    13261 tcaacatgac gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt
    13321 catgggagac gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct
    13381 caaatagatg tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag
    13441 tctgctgatt ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta
    13501 tatgatctcg atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa
    13561 tttgagccat caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt
    13621 ttttttcact ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt
    13681 taaaagctgg tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt
    13741 tttcattata ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga
    13801 tcgtattgct gaaagcgttg acgctctcaa tttggaccac tttatcgctt gtttcgatcg
    13861 ctacatgcca aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag
    13921 ctagctgacg ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat
    13981 tgattgcttc ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata
    14041 aatgcgtctg catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa
    14101 cgatttttgc tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt
    14161 ctagtggttc ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata
    14221 aaatattgtc tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga
    14281 tcacgttctt ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa
    14341 acacaccact tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc
    14401 taagattggt ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc
    14461 ccgtgtaagt gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc
    14521 gaatgacatt gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca
    14581 accctaaaaa agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct
    14641 ccaatccagg taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca
    14701 cataccattc aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat
    14761 agccaacgat cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga
    14821 tgatttcatt cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg
    14881 tattcaaact ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca
    14941 caccaatctg tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat
    15001 aatcgatcgt attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt
    15061 tgctaatata ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct
    15121 ctccacgaaa atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt
    15181 aaaaaatcaa ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag
    15241 catttctaaa aagtgtatag ggttgctgtg acaggatata actatacttc ttcagtttga
    15301 tcgaatgttt cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc
    15361 attttgtgtc ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg
    15421 cttcatatac gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga
    15481 attgtattct agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat
    15541 ctaaatcaaa tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa
    15601 aaccagagtt cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag
    15661 aaagctttcc aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac
    15721 tgttggtttc ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt
    15781 cggaagtttt tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga
    15841 ttgaactttt tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa
    15901 ccatcaaaat gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc
    15961 ctcataaggt tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc
    16021 tgcccaaata gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc
    16081 tgtcccacct tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg
    16141 gttcagatct tttttaggaa tccccaatcc ttgatcggtg atacttaaaa tcacgttatt
    16201 atgcgtttct aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt
    16261 attcatgata ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc
    16321 tctttgcgta aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc
    16381 aaaccggtca agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg
    16441 ggtattacct gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg
    16501 gtctgtttca tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc
    16561 accttcgctc aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg
    16621 ggaaacatta gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc
    16681 atgcaagaca gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc
    16741 tactcgcaag atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc
    16801 gtttggatct tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa
    16861 aatcgattgt ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt
    16921 gatcgtaatg actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag
    16981 cacgctatcc aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc
    17041 agctaactga ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac
    17101 tttccgactg tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg
    17161 ctgtgtaatc gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag
    17221 tgaggcagta aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt
    17281 acttttgacg taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac
    17341 attgatattc acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc
    17401 attgatatca acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt
    17461 tcctcggatg atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact
    17521 gttttcaata aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg
    17581 attcaattcc gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat
    17641 aaatgtattg atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat
    17701 cgagatcaat aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa
    17761 gcggactttt cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac
    17821 cacgacgtgt cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac
    17881 gacgtacagt tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt
    17941 gtaataaatg ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt
    18001 cgaattcacg atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg
    18061 gatggatcgt caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg
    18121 cagccgtggc accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa
    18181 atggcttggt cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag
    18241 aatctttggc tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa
    18301 cttctaagcc gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct
    18361 cagcaacttt ttccaaggct tcttccccat catatgcagt ataaacgtca tagccttctt
    18421 tggctaaatt gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt
    18481 ttttcataaa gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc
    18541 tgcttttgtt tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat
    18601 actttattat tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt
    18661 gtttcctttt tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac
    18721 cagtgtggac tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga
    18781 actctctttt aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa
    18841 cagctaagtt gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat
    18901 ccaaactttt tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac
    18961 atgagatgat tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc
    19021 gtccaccttt ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga
    19081 ctaactcatt cagattactg atcagttgat cccattctac tccttcttcc agccattttg
    19141 ctgcttggat cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg
    19201 cttccaattc ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc
    19261 cactggaaat tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga
    19321 tttctgtgat cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt
    19381 gatagatctc ttcaggggta atgtcaattt tatctgtata tacctgatct ttataaataa
    19441 ttttcaatgg gatcatatac atctcgtatt tttttatcaa ttcttctgga acatccgtac
    19501 ctgaatctac taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat
    19561 tcggtttcta gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc
    19621 gctcttacag caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta
    19681 ccttgtgcta attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta
    19741 taggcttcac gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga
    19801 atagtcaata cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta
    19861 tatctttttt taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa
    19921 gaggtaagca atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac
    19981 gttctgactt ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg
    20041 aatgtttcta gttccttccc ccactccatt aattgctgac gcattttttc catcataacc
    20101 gctccttttt tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg
    20161 ctttaactag gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc
    20221 cttcttgtct tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta
    20281 aaaaatgaaa aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt
    20341 atataatagc gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt
    20401 tttctgattt tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa
    20461 gtactttttg tacgggccgt tagctcagtt ggtagagcag ctgactctta accaaatgct
    20521 ctatttgtga aaaaagtttt ataaagcttt ataaataggt ttttagtttg tgaatttttt
    20581 cattgccaac atactgccaa caaattgcca acaaaaaaat tctaaaaaca agaagcgata
    20641 ccccaaagat attgtttttt gtttttttat tctttaaacc caaatgcgtt ttctaatgct
    20701 tctgctgcac gatcttttac tcctgcggtt aaatgcgtgt ataaattagc agttgtcgaa
    20761 tacgatgcat gtcccagcca ttcttgaatg gatttcatat tctggccact atctaacatt
    20821 attgaagcat tagaatgacg caagtcatga aagcgaattt ttttcaatcc atttttctta
    20881 atcaacttac tcaaacgatg cgttacatat tgaggcttca tcaaccgtcc atcttccata
    20941 gtaaatacgt actcattatc ttctaaaata taatcatctc ctaaaagaat acgattatct
    21001 tgttgccatt tagcttgatt tactaatgct cgttcaatcg tttcaaccaa aggtaatgta
    21061 cgatagctgg aagagttctt tgtttgatct ttctttatta actgtgacat attctctgga
    21121 ttttctatga ttacatggtt tatcatgatt tttttgttta aaaaatcaat agcagaccat
    21181 tttaatccta aaacttcact tcttcgcaat ccataaaatg ctgttaaaag aactaccaaa
    21241 tagagttttt cttctgacaa aatgtcaagt aatttcaaga tctcttgttg attataaact
    21301 tgagaaacaa attgtttctt ttttggcttt cttactttat ctgctacatt tgtacttatc
    21361 aattctgtaa taacagcctc ttgaagagct ttgcgtataa atgcgtgata atgaataacc
    21421 gtatttgctg tacagttatc atttaacata tattgataaa actcttgtaa atgataaggc
    21481 tttaacctat ttagtttaat ttttttagga taaaaaaact tgtaaattcg attatggacg
    21541 taatgcttat acccaacata tgtgttaaat gctacttgtt tttttgatac ttccaaataa
    21601 ttaagcaaat agtcataaaa caataaattt ggattagcac attcacattc catagctaat
    21661 tgactttcca attctagtaa aaattcatct ctaatttttt ctgctcgttt tttatttcct
    21721 ttctttttta attttgtatc tttccacttt tgtctaattt ttccgttaga gtctttataa
    21781 gatacaacta cttgtaatgt tcctcttttt tcaattatgt ttgctcttaa tttcaattca
    21841 atctctcctc ttaaactgaa atatcggcaa aaattttgac tattttatta tagcatgatt
    21901 tttaccttca tcttgtattt tcaatatata ctcaatgaca gcttttttag gtattttgta
    21961 ccttgctcca atttttatat tttctattga tctatctttc agcaaattat aagctttatt
    22021 ttttccaata tttaacattt tagttaaatc agtaattttc aatatcgatg gataactttc
    22081 aaatattttt tcataatctt gaaaatccat aaaattcact ctcaattctg ataataagta
    22141 ttgcactttt ttattttagg tgttagaata acaaagtatt aatttgggct gttagctcag
    22201 ttggtagagc agatgactct taatcatcgg gtcgcgggtt cgagcccctc acagcccatt
    22261 gggtgccaaa cccttaaata cagtgtaaac gttggtgctt agcatttaga aaacgtatat
    22321 actgtatttt tttgttttca aaagagactg tagtacccct ttttttcact aaaagcccac
    22381 tgtagtcatt gatatttttg ataagaaagg aacataatta tgcgtaatga aattgctaat
    22441 ataacaataa tccgagaaac tttacaaaac catactgcaa atgagatctc taaacataca
    22501 ggattaaatt tgagtactat aaaaaaacta aaaagtggag aacgttcaat agaaaaacta
    22561 aatttacatg atgctatttg cctcactgaa tttggtctta aaaataatag aaaaaatgtt
    22621 gaaatcaaca tatggaaata attcttaatc agctgctttt gtcaaaagca cataggaatt
    22681 tcacctctct ccaagtttac ggcgagcctt acggaagtat cattatccca acttcaaatc
    22741 atcgcttttt attgactata aaaattattt tagaatgttt atcgctcctt ctttacagta
    22801 agaaatcatg gcgcaactaa aatatgctcc ttgaagtgtt aacgtactga tagaatgtat
    22861 ttaaatttca atgatcaata ggttttctgt ttaaaagaat ctactaatcc tttaactatt
    22921 agaagattct tttaaacaga ataagtggaa aggggacttt ttagttagtt aattttaaat
    22981 tttaagatcg cttctatcaa tttaatttct agctgtctta gcatatactc atctacatag
    23041 gtaataaatt gccccgcctc attaaatcca tttgtcaaac ataacttagc tatataacta
    23101 tgataatgtt tgagtatcat cttgattgaa attggatctt cttctagagc agattggata
    23161 atggatagag gaactaatgg atactgccgt tttttggggt tgtctaccat aagtcatacc
    23221 ccatttttaa ttgttcaact gccttctttc gttgatacca gataccactt gtactcatat
    23281 tgaggatttt accgatttca gtatcattaa aacctgcaaa ataataaagt aggataacct
    23341 ttcttcttat ttccgataaa tgatcaatgg ctgccgataa cttttgatct gaaattaata
    23401 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gagtacttac
    23461 cttcatttga attcgtcaat ggttcctcat tatctgttat gttaatgttt cgttttcgaa
    23521 tagacttagt ttcgtttcgt aatacttttt tacagtaggc atcaaattga tgttgtatag
    23581 atttctcatt gtaattttgt tgcatgataa atatgctcct tttttcccct ttccactaat
    23641 acaacaatca gaaagcgcct ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac
    23701 agaaaatctt tttataaaaa aaaggagttg gcaattaagc agccactcct tttttatatt
    23761 ccttataaaa ggtaaattat tttttataac tgaaatttgg tattgatgac catcgtttct
    23821 ttagatagtg agcaatcatt ttaggctttc tatctctagt aaagattcct ttcttatttc
    23881 cttgcactct taaaatccct tgacttgttt caaaatctgc aaaattccac acttgttcac
    23941 caacaaaatt gtcacattta tctattactt cgtgatttgc ttcgtaataa cccacttgat
    24001 actcttctgt aaacattgtt ggtactgtat catgaagtcc agctactgta tctgctccat
    24061 attctgtaaa cattattggc gtatcgggac atctttccat ccatccatct agctcttttt
    24121 tcaacagata ttttgcatgt tttaggtctc cacctgaaac ataccaacca taatagcgat
    24181 ttaaacataa aacatcgaca atatctccta ctttacaaat atctggtgtt gactttaagt
    24241 aagtaacgat tgttacaggt ctcttctgag ggtcaagttc tttagtcaat tcaactaaag
    24301 gttcaaaata ttctttagcg ccttcagaat ccgaatctgg ttcatttgca atagaccaca
    24361 ttacgacact cggatggttc ttgtcgcgtt ctatcaattc cttaatcgcc attttgtgag
    24421 cattttctgt ccgtatttct ttccaagtat catgtttttc tacgttgtca tctaataaag
    24481 tagccatgaa attcaaatgt aatcctactg caggtacttc atcaataact acaatacctt
    24541 ctcgatctgc taagcgcata atttcttctg aataaggata atgtgaagtc ctaaatgaat
    24601 tagcaccgat ccatttcatt aaattaaaat ctttgatatt tactgcttca ttcaaaccac
    24661 gaccattaat atacgtgtcc tcatgcttcc caaatccctt aaagtaaaaa ggtttattat
    24721 taatcaaaaa cttcccattc gaaacctgaa tactacgaat gccaaattct tctgtgtacg
    24781 tatcaatcac tttattttcg ttaattaaac taactttcat actatataag taagcattta
    24841 gaggctccca aagtgttggg ttcttaagtt gaatcactcc ttctttcccc ttagcttctg
    24901 ctataagttg agcttcttcg tcatagattg ctattagaat ttctccggtc cattctgtat
    24961 cgatattaaa ttctacattt gcatctgaat caattatttt tgtattaata gtgacatcct
    25021 ttatatagtc tttcggagtt gtatacaatt taacgggtct attaatgcct gcataattga
    25081 aaaagtcaaa attaaccaaa ttttcttttt ttatgtctcc atcttcctgt atcgtttctt
    25141 tcaaaatccc tacaggtaaa gtgctatcgt caataacatt gtttacagcc actgtaattc
    25201 tgttacttcc atcttttata aactgattaa tttctgattc aaatggcgta aaccctcctt
    25261 tgtgttgatt aatcaatttt ccattcaaaa atacttttgc ttcatgtgtt gcagatccaa
    25321 aacgaagaac taatctttct tctaataatt ttttaggaag aatcacttct ttttcatacc
    25381 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagaag
    25441 gaactgaaat tggataactc tctttttcaa ccattggttc taaagcaagt tcttcttgta
    25501 agtcatcatt tgtatctaac ttaaatctcc aaagaccgct taaatcaatt acttgcctcg
    25561 tctctgtcat aatgggatat aacatgtaaa tcactcctat ataatttaaa tattaaataa
    25621 atgtatcctt taacccacaa taagcatcta ttggacttat tcccttaaat tcagaaaggc
    25681 ccattagttt gtccactaat atttctaaag tttcttgttt tccatcataa gtattgatat
    25741 aagtttttac ttgaggcaca tcagctaaat gaaacggaca ctgtacagat acaaaaattg
    25801 ttggaatttc atgaatataa aatggaatat ccggagtacc tttacttgct ggccaaacaa
    25861 ttctttgaac tgtacctggg ttaacattag ctatattaat aatcaagtca tatttttcag
    25921 ttaaatcttt gattggtctc ttagcagcat atattccagc tatattttta attttttctt
    25981 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
    26041 ttgctgacaa aatctgtttc aataaatcaa tggcctgagg tctatttcca gcaactactt
    26101 caccaaaccc accttttacc ccatgtacat ctaccagtaa aatcctttta tatttttctg
    26161 gtacaatagg ccaaatatct tgtttatctt taactagtgt aattgcttga tcagctacct
    26221 ctttaaaaat tgatttattt tcaattaaac caattttttc catagcttct tgtttcggta
    26281 acaaaatttt attcttttct tttttatgca aacctagttt agcctttaag cctaaaattc
    26341 ttcttaatgc atcacgtaaa cgctcttctg aaataattcc tttgtaataa ccttccatca
    26401 tataattaaa gtcttcatct ggatcattaa aaaataggaa tagatcacat ccagccgcaa
    26461 tagaagtggg caataattct gaccgtttca ttgccccagt cattcctaac atatgactag
    26521 catcagtaac tactaatcca ttaaacccga gttttcctct aagtaagtca gtgattaatt
    26581 cttttgataa agttgcagga agtaaatctt catctttcag atcaagatta aaatgttttg
    26641 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
    26701 ctgagtatac ttttccaaaa gaattatccc attcttctgt acttaaacta ttaatactaa
    26761 aagatagatg ttgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
    26821 aaattccact ctcttgaata cctttcatat aagctaaact aagttctaaa actttatcag
    26881 ggctttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
    26941 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaattcca gaaactctcc
    27001 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
    27061 cggtaccatc tgtacaagct ccgttaccac ctgcttctgt attagcagca attaataaag
    27121 gaattttgct ttctgtttgt aggatatagt tttgatcata tacctcatca gcagttcctg
    27181 gattatatcg tacagctcca atatggtact gattgaccat actacttaag taatttttat
    27241 cacgactaga tcccatatta acaaataatt gacctatttt ttcttctaaa gtcatagcat
    27301 caatcgtctc ttcaatccaa gaaatagcat cttcatctaa atgatatggt ttttttcgta
    27361 agtctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag
    27421 tatctatata tttttgttca atatctatat actctcccga taaacattta aataaaatat
    27481 cagttcttaa gttttcccat gattcttttt ctttattaac caaaatagga taaaagaaaa
    27541 ctttattctt atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat
    27601 ccaaatcaca accattttca attacacttc gaataatgtc ttcttttttt cctttatctt
    27661 gacaacacag ttcatcaact aaggataata attcatgtct ttcccattct tcttgaatag
    27721 cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag
    27781 tatctctagc acttaaaaag ggtttatcta atcctttagc ttgactaatc tgtcgattga
    27841 cttcttccga ccattttaaa gcttttaata aatcctctga ctgagagtat tctatttccc
    27901 tcttcaaaga tggattagac aattcttttg ttacagatac ccagtgcttt aatcgattaa
    27961 tcgatggtac atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac
    28021 gagtctctga atataaatta atattattcc aatcctgtag gaatttttcc ctatgatgaa
    28081 catcaaaaat ctctgcagca attggcccga aaaagagatt gtgtttataa gtcattgtat
    28141 ccatgacaca accatctgaa tcaatacaaa atatccaact gttcatgttt tttctcctag
    28201 ttatgttttt gatttagcaa attataattt tctaatactt tttctttaga taatggatat
    28261 acgaaaaata aaatcaatcc tacaacaaag aaacaaactg ctggaatcaa cgttgaagag
    28321 ttatacagct tttgtaaaac ttctccactt tgtacttttg ctaacgaatc ataccctatt
    28381 atagaaagag tccaacctcc gacaccgcca gctaaagctt gtccaatttt tcgggcaaaa
    28441 gaatatactg cataaatagt tccatcttct ctttggccag atttcacttc ttgatcatcg
    28501 attacatcag taatattcgc ccaaatgact gtattaaaaa tacctaaccc catatatcca
    28561 acagaagtaa gaactaagaa tacatacata ttactaggct ttatcaaaaa taaaagtaaa
    28621 tatacaatag cagagaagaa catccctact gacgccaatt cttttttacc gtatttttta
    28681 ctgaaaatta aacttacagg tgaaacaagt accaacatca aaataggatt gataaagttc
    28741 attaaaacta ttccctgtgc attattataa taatctggaa atacataatt gttcatactc
    28801 attgttaaaa gctgagcaag caacaaaaaa atagctgcag ctattataga aagcaacgct
    28861 ctatttttag ctaatccttt taaagatttc aacaatgaaa cttttaactg cgattcatca
    28921 cttttaaccc gttctacagt taatttataa catattacgt aacaaataac tgcacacact
    28981 gaaaatactc ctgccacaat tgtaaaagtt gatccaccac gtacaatttg agctccagaa
    29041 ctatctcttg tataaataaa aattggtgtt aacacgccaa tgaacatacc tgctaaggta
    29101 gctcctatac tacgaaatgt tgataacgct gtcctttctt ctgcaacaga agtgatagct
    29161 gatgccattg aaccgtaagg aatattaata gatgtataaa aaatacttcc ccatagtata
    29221 tatgttacat acatatatat aactttaata ctcatagaag ctgatgccat tgatgtttga
    29281 tacattaaaa aacttgctaa agcgactgga ccactcatcc gtaaaatcca cggtttaaat
    29341 ttgccagttt tagatggttt tgatccatcc acaatacgcc ccattgttat gtctgtaact
    29401 gcatcaataa atcttgctac taaaaataat gttcctacgg tagctccgct tatccccaat
    29461 acttttgtat aaaaaatcat taaaaaagaa ctagcaaaaa tgaaagtgaa atcattccca
    29521 aaatctccaa acatatatcc tattttgtct cttagaccaa attttctttc catataattt
    29581 gcgctccttc ctattgttaa gcgttttcat tctgttgaac tgccaactta cgtactatac
    29641 tctgaagtat aaagagttag tatttatttt ttacatttta tagtattatt tattttttct
    29701 cctaataatt ctttatctca taaaacttat ctatatattt actagctatt cgagttactt
    29761 cttcaaaatc acctttttca acaggagcca acaaattccc acctacacca actgcaacaa
    29821 cgcctgcttc aaaccactct tctaagttat ctagacttac tccgccagta ggcattatat
    29881 ttacatatgg taatggagct tgatacgttt ttacaacaga tggaccaaaa gcagaacctg
    29941 gaaatagttt aatgatttct actccagcgg taagtgcctc ctttatctca gtaattgtca
    30001 tacaacctgg taaataagga atttgatata aattacaaat ctctgctgtt tgtcgatcaa
    30061 aagatggact tacgatatat tctgctccag ccataattgc aatacgagca gtaaacgcat
    30121 ctaaaactgt accggctcca attattacat ctttttgatc agcatatttt ttagcaattt
    30181 ctttgattat ttcttcagca ttcggaactg taaaagttag ttcaatccct ttaattccac
    30241 ccgcagtaat tgcatttatt gctttaatgg cctcatcttt attttttcca cgtacaaccg
    30301 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    30361 tcttttatgg atacatactc tagctagttg tactaactta caataaataa atttaagctt
    30421 ttattttttg tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt
    30481 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcgacc
    30541 tgctttaacc cactcgctta gtaattgaca taatactcga cggaaatact catggcgagg
    30601 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    30661 ttgcgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    30721 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    30781 tggaagccaa tcggttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    30841 ttcagcagtg tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    30901 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    30961 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    31021 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    31081 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    31141 accgtgatct gctaatcgac agccaatttg atgaaaataa tccactcgtt ggctcaatgc
    31201 ctgcttcaac ccattaaagt ttttgattgg tatacccgct gcttgactaa gttttaaaat
    31261 atagttcgaa aatcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    31321 taccttgaaa gaattatcct gtttagaaag ctcagtatga tatcgcaagt catctgtagg
    31381 atcatccgtt gtacaaatgg ctttcacatc catcatcttc agcaattctt ttgggcgata
    31441 ggaatccgtt tggatttttt cgttggcacg tcgccaaatt tctggtgcat tcttctcaga
    31501 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    31561 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcattacc
    31621 tgcaccactg atcaactctt cttctatccc attagctcgc atcaatcgcc atttataatg
    31681 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    31741 tagcaaacat gctgggtcta aatgacaatg ataatcgatg attggttggt gttctgcaaa
    31801 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    31861 atttaagcac tcctttcctt cccttagttt ttagaagaga aaatttttct tctaaataga
    31921 ctccattata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    31981 aaactgcacc tagagctcgg tcatataacc cataaccagg tctaccggtt tctccccaaa
    32041 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    32101 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttca taaaaatcga
    32161 gtccattatc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    32221 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    32281 tacataacgt aataccatta gaaggagaat ccactacatt acacaaccat tctaaatcgt
    32341 cacgattttt aacaatacga ggtaatccga aaatagaata tggtggatca tctggatgaa
    32401 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    32461 agttctctcg caatttcgct tcatcaactt ctttataggc ttctaataat tgcttgattt
    32521 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    32581 ttctaataat ttcctctgga tcagtaggaa tattttttga aatataggct aacgtagaag
    32641 acccatcatc taaatgataa tccaaatctg attttaccca atcaaagact ggcataaaat
    32701 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    32761 cgatgtattg atctcgtgtt ggcaaaccaa ttttgatatc atcatgaaca ttaacactct
    32821 caataacttt caaggtaagg ccattggctt cgacttcatc ttttaaagct ttaattcgtt
    32881 ctctcggcca gacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    32941 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    33001 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    33061 aactgcttga ggacctacta gcatttcgca aaagattttt tcaattttct ttcctatctc
    33121 aacttgatat aaatctaaac caaatatttc cttattttct aagatttcct gtaccgcaca
    33181 atgaatcttt gcaggatctt gttcacctaa tttcaatact gagaattttt cctgcaactc
    33241 taccaataaa ggatcaggac ttggcacaaa attttttcct tgatcatcga ctgccaataa
    33301 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    33361 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    33421 tgttgcaatt cgttgcggtg tatctggaat atagggatta ggcaaacgtt tattgattac
    33481 ttcatcaata aattgttttg gatcaataac gcctggactt tcaactactg gtaagccttc
    33541 tacataacct atccctttaa ttaagccaac caaatcatca tcttgcattt ctgcctgaat
    33601 tgatttatac cccaacaaac aacctgttat tgctaatgcc gtatggagcg gatttaaaca
    33661 tgtcgtaacc ttcatgatat ctgctttatc aactgtttct ctatctgtca taatcacacc
    33721 agccgcttcc agtttaggtc gaccatttgg aaaactatct tcaatgacta gataatggac
    33781 tttttcagta ttggtaaaag cagcgatatt ggtatttttc gcagtatgaa ttatatctaa
    33841 gtcttcgaaa cccgccttca ataaatccat tttgacactt tccgaaggat ttggcgtaat
    33901 tctatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    33961 tttttcagaa acgtatcctt ttttttgcca agcttctgca atcataaaaa tgctatcttg
    34021 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gtaactctcc
    34081 agcattaaac ctgctataca acaaggatgt cacaatactc atcgtatgtt gaggattggt
    34141 aggtccagat tttatatctg cttccactac cgaaaaaaat tgtcctttag aatcttttaa
    34201 actgtatcct ttttcagtaa tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata
    34261 tttaaccact ctttgatagt ccgttatatt ctttggattg caatataaac tatcagctgt
    34321 tactgctaaa agtctttttt tgagttcacc atcttcaccc ataacaatct ccaaaatatc
    34381 attgttgtag tcatgataag ctttttctac tacttcttca tcaaatgtct cacacacaac
    34441 tactccggat tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    34501 tcgataaaga tttcctccac caaaatgaag ccacactgga tgtttctgac cttcttgttt
    34561 gaccgcttcg acattaaaat acggtagttg aacttgcatt ttgttcaatc cttcgataga
    34621 atcttgccag tctttaatca aaaccatatt tacccctcca tactgatatg ttagtttccg
    34681 ttacaattgt agtataaaat cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt
    34741 cttttttcta ccatgtcaga attattgtgt ctttttttag taaaagcaca ccaatatata
    34801 ttttttagaa ctaacttaaa ttaaagctta atttctcttt gatcttattt gagtattttt
    34861 tgttttttta atagctagta tgttactttg aaatttgata ttatataacg agcatagaac
    34921 taaaatatca aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag
    34981 tctccaaaat caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc
    35041 tggaaaaaaa gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat
    35101 acgtgaagca ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg
    35161 aaattatatc tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca
    35221 tttagaacga caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt
    35281 tttagaaact ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt
    35341 ttttcataat gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt
    35401 atggcaatgg ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa
    35461 aacggaagga ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt
    35521 gaccatgcaa gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct
    35581 tgatgaacaa aatgttgtat tgactactca cccaaaatat tttaaaaata tataactgct
    35641 aaatttcttt tttttaattt atttacattt ctgattacta cagaaaacta actacttttt
    35701 ttatgtaaag ttctgtcaaa tatagtcact ttatattact ttctcttgag taaaaaaaca
    35761 tgtgaggtcg taaacaaaga ttgaactatc tacattcata ctctataatc tgtttatttg
    35821 cagactgata atagcaaaag aaaacttata atcaaataat atcaacattt gatcacataa
    35881 tttgagattg aatgctgatt tacctatgtt ttacgtaatg gtctttagtt atttttgctc
    35941 ataaaatgca taaaacacgc gtttttatgt gtgcttgaaa tttcttggct ctataatttc
    36001 taagactgat aaaaatattc tatcggttct ttttgcaaat aaaaaaacat atagaagctc
    36061 cattagattg aatcaccaca acacaatcaa aggagcattc cgtatgcttg attttcattc
    36121 tataaaacta cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata
    36181 tccgaagtac cattgtttca tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt
    36241 cttatatcgc tctgtcagtc ctattaataa agtactgttt tgttttcaaa aaaactatta
    36301 cgagtacttt ttaagagaat gccttgcaaa tattcatcac acaccgttat ataagtcaaa
    36361 tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca gttaagtagc
    36421 aacttaattc tcggacttgt tctactctag caaaatttat tttcatcaac ttacgtccaa
    36481 atgttttttt gaataattac ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc
    36541 actagctttt ggcgtccttc aggatttcta gtatacacca actgacattt gggcagcgac
    36601 gattgattta attcgtttag aaaaaggtta gcgatttgct tatttcggcc aaagcgttct
    36661 ggtaccgtca gaactatctt tttcccttcg attaaatacc gcggattctc cagcggtgct
    36721 aaaagctccc tcatagcttc agtaaaaagc tgtccttctc gatagccagc attctcaaga
    36781 tagcatctaa attcctgctg atcagccgct acgattactt ttctatcact aacttcacca
    36841 aggcgctgca acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc acattccttt
    36901 tcccatagct tttgtcgcct acgaatttgg aaatattcca gcaagttacc tttaaatata
    36961 gctgaccaga agcttagagg ctctatattt tttactacta tcggcaatga agtttcttgt
    37021 ttttgcttta cctgatcgac tttcctaacg acgattggca tagataggtg ccagccatca
    37081 gctaaagcag tactccattg ttctttcaac cgacttcgtt cacaagaaaa atagaacatc
    37141 gactgagtat aagtttccat cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg
    37201 ccaccactct caatttttgg tgaagattca aaggtcagac ctgaaatatt gcccaaacgc
    37261 cgtagcattc ttttcaaatc aggctgttca gacagaggaa ctcccggtaa tccaatcgct
    37321 aaatgccaaa tgttcgttac tttattttgt gcacctaggc gtaagccccg ccctcgtagt
    37381 tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga tcaacagatt aattgcagga
    37441 caatcccagc cttctcctag caaactaacg gttcctacca aaatattcaa cttcttttcg
    37501 gttaataggc gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa
    37561 taaaaataac ccgacatata aggatgttca ttcatacccg tcgctagttc tgccagctca
    37621 acagttacta gcattttcgg taacagtaag aactctccac agatggcacc gatttgctga
    37681 gtgttaccta atagacgaac aacttcatca aacaaaggaa ccacgccata agctaaggta
    37741 ccatcactgc ctgctaatac ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt
    37801 tcttgcttca gctgaacttc ttcttttaac aaaatttgac aaactgcttg ctttttctca
    37861 ggaatcgcct gactaccaac agtgtctgga tactctggaa acaaatgcag cgactcatca
    37921 aacaatcatc cagctttttt caattgataa aaaataggca attccggttg atcttcaaac
    37981 atctgctgat aaatagtgat cagctctttt ttacctaaag atggaactga agctttttta
    38041 ccttgctgct tagaactgcc taatagctgc caatgttcgg gtatcaattc atagttatga
    38101 tgattcaagt aaattaatgc actagtgaac agctcaaaat tttcataaat ttccttcgaa
    38161 tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat aatccaccgt ctcttggctt
    38221 tccattaacg cttccaccag ctcattttgt ttctctaaaa aggtaaaata atgctgagta
    38281 gtttcctgat tagccggaat caaatataga aaatcctgat gaggacatag aacctcctcc
    38341 tttaccaact cgggaatgct aatttcttcg tcaatcaggc cattttaact caatatattg
    38401 cttccactcc gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag
    38461 attcggttgt tttttcttca agtcaatcaa caattcaccc cagctttttt ttaaatgatg
    38521 agcttcatca aaaaactaac agctccagct gatcaccata agttggctcc tccttcagtc
    38581 ggttgtaaat ggtctgatac gtttccactg taatatgact aggatgttca atcgccccag
    38641 aaagatagca actatctgct ggatttacta agaaatattc ctcgattgtt gttaaccact
    38701 gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca
    38761 tctcaattcc taaaaaggtc ttgccagccc ctggtggagc cactagatgg actgaattgt
    38821 tatttaaata agtagcaaag ttagtcaata tacgcttctg atagctacgc cactgaattt
    38881 tttcatcaga tagccacatc ccaggtcccc ctctcacgta ttttctctat cataatctaa
    38941 atgaatacag caaaaaaaag atttcttcaa gaatatgtgt ttttatagta atattttact
    39001 tactctaaac tcaaatgaaa aggtaaaaaa actggtagta caatctttta aattctcatt
    39061 gttttgtacg ttctttacta tcaaaaaaac aaaaaaaacg cgtttctacg cgtgaccaaa
    39121 attttt
//
LOCUS       DAJSBC010000023        37268 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSBC010000023 DAJSBC010000000
VERSION     DAJSBC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.023"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..862
                     /locus_tag="ONT65_001193"
     CDS             2..862
                     /locus_tag="ONT65_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2328838.1"
                     /translation="MDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIE
                     KEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFI
                     SIKFRKKRNI"
     gene            complement(1286..1456)
                     /locus_tag="ONT65_001194"
     CDS             complement(1286..1456)
                     /locus_tag="ONT65_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2328839.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1650..1829)
                     /locus_tag="ONT65_001195"
     CDS             complement(1650..1829)
                     /locus_tag="ONT65_001195"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328840.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2216..3328
                     /locus_tag="ONT65_001196"
     CDS             2216..3328
                     /locus_tag="ONT65_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2328841.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3406..3699)
                     /locus_tag="ONT65_001197"
     CDS             complement(3406..3699)
                     /locus_tag="ONT65_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR2328842.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3867..4181
                     /locus_tag="ONT65_001198"
     CDS             3867..4181
                     /locus_tag="ONT65_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR2328843.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4202..6181
                     /locus_tag="ONT65_001199"
     CDS             4202..6181
                     /locus_tag="ONT65_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2328844.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6194..6520
                     /locus_tag="ONT65_001200"
     CDS             6194..6520
                     /locus_tag="ONT65_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR2328845.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6536..7054
                     /locus_tag="ONT65_001201"
     CDS             6536..7054
                     /locus_tag="ONT65_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328846.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7054..8406
                     /gene="celB"
                     /locus_tag="ONT65_001202"
     CDS             7054..8406
                     /gene="celB"
                     /locus_tag="ONT65_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2328847.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8490..9995)
                     /locus_tag="ONT65_001203"
     CDS             complement(8490..9995)
                     /locus_tag="ONT65_001203"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR2328848.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10087..10677)
                     /locus_tag="ONT65_001204"
     CDS             complement(10087..10677)
                     /locus_tag="ONT65_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2328849.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10856..12502
                     /locus_tag="ONT65_001205"
     CDS             10856..12502
                     /locus_tag="ONT65_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2328850.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12551..13270
                     /locus_tag="ONT65_001206"
     CDS             12551..13270
                     /locus_tag="ONT65_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2328851.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13749..14696
                     /locus_tag="ONT65_001207"
     CDS             13749..14696
                     /locus_tag="ONT65_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2328852.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14708..15628
                     /locus_tag="ONT65_001208"
     CDS             14708..15628
                     /locus_tag="ONT65_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2328853.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15669..17126
                     /locus_tag="ONT65_001209"
     CDS             15669..17126
                     /locus_tag="ONT65_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2328854.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17471..18085
                     /locus_tag="ONT65_001210"
     CDS             17471..18085
                     /locus_tag="ONT65_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2328855.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18078..19802
                     /locus_tag="ONT65_001211"
     CDS             18078..19802
                     /locus_tag="ONT65_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2328856.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19799..21250
                     /locus_tag="ONT65_001212"
     CDS             19799..21250
                     /locus_tag="ONT65_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2328857.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21723..23876)
                     /locus_tag="ONT65_001213"
     CDS             complement(21723..23876)
                     /locus_tag="ONT65_001213"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR2328858.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23878..24924)
                     /locus_tag="ONT65_001214"
     CDS             complement(23878..24924)
                     /locus_tag="ONT65_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2328859.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25074..26363
                     /locus_tag="ONT65_001215"
     CDS             25074..26363
                     /locus_tag="ONT65_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR2328860.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26376..29078
                     /locus_tag="ONT65_001216"
     CDS             26376..29078
                     /locus_tag="ONT65_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2328861.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29063..29920
                     /locus_tag="ONT65_001217"
     CDS             29063..29920
                     /locus_tag="ONT65_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2328862.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29991..30890)
                     /locus_tag="ONT65_001218"
     CDS             complement(29991..30890)
                     /locus_tag="ONT65_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2328863.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31059..33230
                     /locus_tag="ONT65_001219"
     CDS             31059..33230
                     /locus_tag="ONT65_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2328864.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33298..33639
                     /locus_tag="ONT65_001220"
     CDS             33298..33639
                     /locus_tag="ONT65_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2328865.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33915..35669)
                     /locus_tag="ONT65_001221"
     CDS             complement(33915..35669)
                     /locus_tag="ONT65_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328866.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35680..36516)
                     /locus_tag="ONT65_001222"
     CDS             complement(35680..36516)
                     /locus_tag="ONT65_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2328867.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36518..37201)
                     /locus_tag="ONT65_001223"
     CDS             complement(36518..37201)
                     /locus_tag="ONT65_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR2328868.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12023 a         6556 c         7646 g        11043 t
ORIGIN      
        1 aattgacggt ttagcaccag gagcatatca gctggtagaa actcaagcgc caacggggta
       61 tgaattagat gcgacaccga tcgagtttga gatcgaaaga agtcagacag cggtggtaga
      121 attgacgaaa gaaaaccgac ttacaccagg cggtgtcgtg ttgacaaaga tcgatgacca
      181 aagcggggaa gtcctgcaag gagctgtttt tgagttacaa gatgccaatg gaaccgttct
      241 gcagtcaggt ctaaccacag gagcagatgg aaaactggcg attgacggct tagcaccagg
      301 agtgtatcag ctggtagaaa cccaagcgcc aacggggtat gaattggatg cgacaccaat
      361 cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag aaaatcgact
      421 tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggagaag tcctgcaagg
      481 agctgttttt gagttacaag atgccaatgg aaccgttctg cagtcaggtc taaccacagg
      541 agcagatgga aaactggcga tagatggctt agcaccagga gcatatcagc tggtagaaac
      601 tcaagcgcca acggggtatg aattggatgc gacaccagta actttcaaga tcgaaaaaga
      661 acaagaagtg gcgatctttt taacaaaaga gaatagaaaa gttgctgata gctcagatat
      721 aagaggaaac aatccaacaa gcagtggaaa taagcatctt ccaaaaactg gagaaaccat
      781 cattagccag tttatattgt caatattggg tgtgctactt gttttcatca gtataaaatt
      841 tagaaagaaa cgaaatatat gagctaaaat aaaaaattga ataagcatag tttttaaaat
      901 caactagtac ccatcaattg aatggaaact accgatttta gaaggggtat acaaacaaat
      961 attagtatcg gagtagagaa ggttgaaccc aaccagtaaa taagtaaaga caattttgtg
     1021 ctgcgttgag aagtgaaaaa taaacctttg tagtaatact agttaataaa aaatatacaa
     1081 aaacactgcg aaaatcattc ttgagtttcg cagtgttttt tgcctaatga ctcaagttta
     1141 ttgggtaata ctttgatttt ctttctacaa ataatcagcg atcagttgta attttttctc
     1201 tagttttaat ggctctttgt caataaggag aaaaacaagt cgggtctttt gtctcggaaa
     1261 gcttcaacca actcgtgaat aaaggttacg cctccttgaa aggcgtggaa aaaccaagta
     1321 atcgatcaat caccaatttc gcatttggta gaacatgttt ggtgagctga aagtaggtgg
     1381 cgttcatgtc tgtaactaag aattctattt ctttaggatt agtgcagcta aggaaatagt
     1441 ttgttaatcg aggaattttt tgtgtaggta aattttctcg attaattttt ctgtctcgcc
     1501 atccgcggaa ataaaactca gtttatcctc aacggaaacg tgagaacgaa attcacaacc
     1561 ctcaacactc aagacagaat tcttttggaa gactttggta aatagcttct caactctttc
     1621 aatgtgcgaa taacggttgt caaagatacc taacaacttt tcgaaataaa aaataacgac
     1681 actttttcag tcagtaaaga agtgatttta tgtctgacat gatttgaaat cgagtgcctt
     1741 ggacggataa aataactttg agcgttccaa tggcttcgac agtttttaca ggtatagcgc
     1801 tgctttttta ggcgtataac cattggcata tgattatatt attcaaagtg gacaatggtt
     1861 tccttcttct tgtttttcac caccattaat ttcctatttc catctaccac agcagaacca
     1921 caatttctac agacacatgg agcagggaag agaactgcag caacgatcaa tgtttttttc
     1981 ttctgtaagg tttcgtaaga gacctctgta atcatcaaat ttttgtctat tgatctcaga
     2041 atttttttga tagaaccatt cataaagcat atcgttctct cagttgttta ttttgtggtg
     2101 atttaatcat acaagaaaac gatatgtttt tttataaaaa catgaatagg aaaaatagtt
     2161 gacaagaaaa tgacaacgcc atatgatgtt ccatagtatt tacaacctgt catagatgac
     2221 tgtaaaaaat aaaagtaaaa agaaaggacg acaacaggtg gatttttatc agtctctaca
     2281 actagatccg tttattttaa aacaaaaaat acgagaagca gcttctaaaa aagaaaagtg
     2341 catgtatatt tgttcattgt ttttacgatc attattcatc gtattgtttg ccatttgttt
     2401 tatcattata attacaacat tatttgaatc gaagcataaa ccatacgctg tggttttatt
     2461 ctgtatgttg atgagtattc gttttgttga ctttggttat aagatttccc attcgatcat
     2521 aggtttagca atcgtcatgt tctcactatt agtagcaccg tatgtccagc tgatcaaatg
     2581 gtcagttatg ggcatgttga ttcattttat tttactaagc agcatattaa tggcaaccgc
     2641 tagtgatccc aaaatgggga atgcttcttt gtacggcttt tcatatctct ttattgttta
     2701 ttcgttacct aaagattcgt tgaacaagaa tttctttaca caaacaagta gcttattatt
     2761 tttgtttttt tgttggtttt cagttctcct ttacagaaaa catcgagaaa agaacaaaga
     2821 caaaagcttg tttaaagaga tttttttgaa aggcatgtat tcgcaggaaa aaatttggat
     2881 gctcatctat gcatttggca ttagtctttt gatcgttgca ggagagtatg tcccttttca
     2941 gcgattgatg tgggcagggt ttgcattttc ttctattgta tctagctatg gattgatgtc
     3001 gattggtttc aaagaacgag cagtagaccg aatcattggc tcattgattg gatgtgtttt
     3061 atttattggt atcagccaat ttattccttt taactgggtc ggtatattag gtggtctagc
     3121 tttaggtgtt tgttctacct atagatataa aacagttttt aattcttttg gtgctttggc
     3181 aatcactgcc agtttattcg gggttccagg agcagtaacg atccgcattt ttgagaatat
     3241 ccttggggta tgtttaggaa tcatgtatat tggggtgact gaaatactta tccgaaaaat
     3301 aagagaaaaa cacggtttga atcattagat gagatcgaat aaaaaataag gaaaaagtga
     3361 agatacacat ggatgataaa ttgctatcat tccatgtgta tctttttacg gtaaatcagt
     3421 gaaacgttcg actttcattt ttaatttata ctttttacgg agttcgttta tttgagccat
     3481 catttcggat ttgtgatgct tttcgattgc ttcttcatct ttccaacggt cgatcagtaa
     3541 aagagattct ggatcttcta gtggaaagta atagtcatat ttctcgtttc cttcttctgc
     3601 acgaatcgct ttgacaatgt ttctttcgac catttcttct gcaaattttc tggcttgtcc
     3661 atttgtactt gtatacaaaa tattcactgt gatactcact aaatcccctc ctgaaaaaat
     3721 tctatcacta gtatatcacg gtgccagaaa ttattattca agatatgata cacatttttt
     3781 ccgcaatgct acggaaatat atattttgaa tagaaaacgc ttacaagata tgcttggttt
     3841 ataaaaataa aaggagtgat tgatttatga aaaaagcatt gattatttgt gcggcaggaa
     3901 tgtcatcctc gatgatggcc tcaaagacaa cggatttctt taagcaaaaa agagaagagg
     3961 tactcgtaga tgcagtttca gctacagaag gcgataatat gattaagaat agtgattttg
     4021 atttatttct gatcagcccg caaacgacga tgtttttgga taaattcaaa aaattaggag
     4081 aatctgttgg aaagcctgtt gttagtatcc ctttccaagc gtatgtccca atccctacag
     4141 gcatccagaa gttagcagag gtgatcgaag agaacatatg atggaatagg agtggatgat
     4201 aatgaaacag aaggaaaaat tgctgttgag atatttgatt gatcacaaga aggagtatgt
     4261 caccagtcaa cgtttagctt ctgaattgtt attatctgat cggacaatcc gtaattactt
     4321 gcagcggatc aaagagttag tagaaaaaaa cggaggtaag atcattgcta aaccaggtta
     4381 tggctaccaa ctgcatattt tacaaagact gacatttgac ttgtttttga gtcggcaaga
     4441 aatagtacct ggatatgcta gagaaccgca aggattttat gagtccgaag atcgacaaaa
     4501 gtatatattg aaccaattgc ttttggaaga tgccattctt tttatggatg atttggcaga
     4561 agagctttat atcagtcgtt ccagtttgac aaaggacatg caagaaatca aggagcgatt
     4621 gattccatat tcactaaaaa tcgtttctaa gcatggtcaa gggacatgga tcgaagggga
     4681 agaaagaaat cgacggcatt ttatcatgga tacttttttt ggaaaccatt atggaaattc
     4741 attgaaagaa tatttagggc aaagccgttt tttcccagac atcagttttg aagagctagt
     4801 cattatcatt ctcgatgaaa caagagaagc taagttaaaa gtttcggatt ttatcataca
     4861 gaatctgact ttacacttgg ctttggggat caaacggctc cgagaagggt ttgaaatcaa
     4921 ggaattaggc atcgaacaag aagtttctaa gcgagtagag tatcaagtgg cgcagcggat
     4981 cgttcaacgt attgaggtaa tcgcaggtgt atcgtttccc gaagaagaaa ttgcttattt
     5041 gacattacat ctcatggcta agtcgaatca caaagaaaat caaaatgatc aggaactatc
     5101 aaaggaatta gcgaaagttc tgaaaagatt gtctcaggaa tttcattttt cattagtaga
     5161 ggatctccag cttttcaatg gactactcga tcatttgtca ccgatgatga ttcgattgag
     5221 ccgaggtatt cgcattgaga acccattgac tgaggagata aagaaagaaa atcccaaagt
     5281 ctttgacgca gtaaaaaggc atttcagcaa catgccggct ttgaaaaact ataccatcaa
     5341 tgaagatgaa tgggcctatt tggctttgca tctcatggcg gcattggaaa aagaaagagc
     5401 tgcgcataaa ctacatgcat tgattatctg tgcaacgggt tacggcagtg cccaactatt
     5461 aaagaatcgc gtagtcagtg aattcggtaa aaatattaca gtggtaagtg taaaaggcta
     5521 ttatgagatc aatgaaacaa ctttaaaagg aatcgacctg attatttctt ctattgatct
     5581 ttcgacaatg tttttcaaaa ttccagtttt acatgtcagt atttttttga atgaagatga
     5641 tgttcgtaaa atacgaaagg tagttggaga atctataccc tatcattcct cagatagacc
     5701 gataccagca ttttctctac agcaaaaaaa gcagatttac accgaacagt tgtctgaaaa
     5761 atttttcaag acttatacgt acgaaccgaa aaaggaagaa attcttcatg atttgctgca
     5821 aaagctgagc ataaatgaag aagaaggata tataagagaa atgcgaaagc agatccagca
     5881 acgtgaaaat atgggacaaa tcatattcag tgatacagtg gcagtcccac atcccgctgt
     5941 tccagttgga atcgaaacaa aggtagccgt tgcattgatt ccttctggga tgcagtggga
     6001 tcaatatcaa gaaattcatt ttgttttttt agtttcgcct tcttatgtcg aaaatgaagg
     6061 aattacggtc gtgactaaag caatcgttag gttagttgac cagctaaaag tacagcagga
     6121 aatcttagct gaacctattt ttgagaattt cagcagtaaa tttatcaaac tgatcgaata
     6181 aggaggaaaa cagatggctg aaaaaataaa cagtgaaaca ctacaagtga cttcttttga
     6241 tatcattttc catagcggaa atgcacggac actgatacat gaagcatttc gctcgatgcg
     6301 taaagagatg tttgaagaag tagaagagaa gttgcgacaa gcaaatgaag agattcttca
     6361 tgcgcatcag tcacaaactc aacttctgaa agaatatgcc agcgggacaa aaatagaaat
     6421 ggaaattatc atggtccatg cacaagatca tctgatgacg acgatgacat tattggaagt
     6481 tgcacaggaa atgaattatt tgtatcaaaa aaataaatag aaaggaagga tcattgtgca
     6541 ggatacagta aaaaggaaaa tcgaattaga aaaaaagcaa cttagtttga aaaacatgta
     6601 tttcaatcgt tatctaatcg tacgttacct gactgccttt ttctttttca tgaacatgca
     6661 atggatgatt ctgcttctat cagctaaaag tttaggaagt agtttaccga tgattctgat
     6721 tttagctatt ttgcctgcag taggagaaca agtaaagctt tatcgaaaac accaaacgaa
     6781 cgttccatgg acaaagtggt attttttgtt ccagagtgtc tttaatttat tagtgattcc
     6841 agtgttgttt acctcaggat ttacattctt gtatcctttt atggcaaata acaatggagg
     6901 acagttattt gttttcattc ttgttgtaag cggtattttt gccagtgcat ttatacagta
     6961 tcgtttaaag aaaatttcac tcaaccaaga ccaacagtat atccgtatta aacaatatga
     7021 aaaagcgtta tatttaggaa aggagaataa ctaatgtttg attttttgca gaaatacctg
     7081 atgggaccta tggggaaaat tgcgcaactt aaaatcgttc gggctgtcat ggcagcgggg
     7141 atggcttcta ttcctttcac gattgtaggc tcgatgtttt tggttttaaa tattttgcca
     7201 ttaccgttcc catttttaga aggatttttt aatgcaactt ttttcaaagt tagtgacttg
     7261 tacatgatcg tcaatacaat gacgatgggg attttatcta tttactttgc cattgtgttt
     7321 gcttatgaat taacgtcaat tgaacgagat gaacaaaaat taaatgttaa tccgctcacg
     7381 ggggcattac tatctgtttt tgcattcttt atgtgtatcc ctgaattgat catgactgat
     7441 ggtaaaatca gcttgattgc ttctatcaat gagacagaaa ctgtagtaaa tggtattagg
     7501 atgggctctt ttgtcgaacg tttaggaact tcaggaatct ttacagctat tattatgtcg
     7561 attattgcgg tcgaattgta ttgtatgtgt gtcaagagaa attgggtcat aaaaatgcca
     7621 gatgttgttc cgccaggagt ttctcgttct ttcactgctc tgatcccaac atttgtcatt
     7681 gcgtttgtgg tgatgatcat caatggatta ttagttgcac taggaacgga tatcttcaaa
     7741 atgattgcta tcccatttgg gtttgttacg cagttgacga atacatggct tggtgtcatg
     7801 gtgatttatt tcttgatcca tgcattgtgg atcgtaggga tccatggagc gaatatcatc
     7861 acttctttct tgaccccgat cgtattagct aatatggcag ctaacgcaca aggcgcaaat
     7921 tatccgttgg caggagaatt caacaattct tatgtaaccg ttggtggatc aggtgcgact
     7981 ttaggattga tcatctttat tgcattcctc gcgaaatctg atcagctgaa agtgttagga
     8041 aaagcctcac ttgtaccagg attattcaat atcaatgaac caatcatttt cgggatgcca
     8101 atcgtctaca atccctattt agcggttccg tttttcttag ctccgatggc ttctgctaca
     8161 ctcgcttatt ttgccattcg gctggaaatc gtcaaaccga tgcttgcaca gatgccgtgg
     8221 ccttcaccag tcgggatcgg agcatttgtt ggtagtggtg gtgattggaa agctgctgtg
     8281 ttagcagtca tttgtgcaat catcgcattc attatctggt tcccatttat caaattctat
     8341 gataataaat tattaaatga agaaagagaa aaggcagcag aattagctag tgaaaagtta
     8401 aaatgatttc taagaaaaaa ggaactgtga caaaaaatca cagtcccttt ttttcttaga
     8461 aatgggaaac cactagtata taaataccat tattcttcaa tcaaccgctt cacgattgca
     8521 taatcccctt cataaggttc atctacacca ttgatttttt gatagggatc cacgcctttt
     8581 cccgcaataa cgactgaatc ttgcggagcg ctttctgcta aagcgcgtcg aatcgcttca
     8641 ggacgatcca tttcataatg aacgatcaaa tctggattag taattgcttc attgatttct
     8701 tccgcgattt ttttcggatc ttcaaatgct gggtcatctg ccgtcaaaaa ggcgatgtca
     8761 gctgtttctg atagcacttt tccaaaatca tgtcgacggg agattgcttt gtttcctggg
     8821 ctgcctaata caacaattac acggccatct ggatgttcat ttttggcaaa tttcagcaat
     8881 gttttcaagc ttagataatt atgtgcatag tccacataga catgcgctcc attggattgg
     8941 atcaattgat ccattcggcc tggtacacga gcttctttta atccgtcttg tgcatcttga
     9001 ctggaggcac ctgttagtgc tgcggcaatg atcgcactgg ctgcattttc ttggttgaat
     9061 cctccagcca gcaaaatatc ataagcagta tcgcagatag ctaagcgatc ttcatgagaa
     9121 gataaagtaa aaccgtgagt acctttatct gaacggatca cttgataatt gctttcttcc
     9181 gaacgaccat aagtgatcgt tggaatctgg aatagatcgg ctgtttccct aagtaaatca
     9241 aaatagtcac tttcatgatt taaaacaact gtttttgaat tcaagatcaa ttgtcgcttg
     9301 caatagaaat agtcatcaaa tgtcggatgt tcgatcggac tgatatggtc aggcgagata
     9361 ttcaagaaga ttccgacatc taaagtcaag ccgtatacgc gttgtgtttt gtaagcttgt
     9421 gaagaaactt ccatcactag atgagtcata ccattttcaa ctgcttctga catcatacga
     9481 tataaatcta atgactcagg cgttgtcaaa tgggatttga aatatgtttt gccatctaac
     9541 gttgtattca tagtagaaag caatgctgtt tttttatttg ttgtgtgatc caagattgct
     9601 tttgtaaagt acgcagcagt tgttttacct tttgttccag tgaatccgat cactttcaat
     9661 ttattttgcg gatagtcgta aaaagccata gcaagaagcg ccattgcttt acggatatcc
     9721 gttacgatga ttcctactgc tgaattttcg tatggttgtt ctgatacata atattgaata
     9781 ccctgttcga ttgctgaatc caaatattct tctttgaaat tcattccttt acagaaaaac
     9841 aaagtagaag catcagcttt tcttgaatca taacttaact gaagaaattc agctttttct
     9901 ggtacatcaa gatgccagcc tcttgttgaa acaaattctt ttaatagatt ttcttttaat
     9961 agtaaatgac ggatgtcttc taaagagaag ctcattgatt gatccctcgc tttattcaat
    10021 ttcttggttt attatagcac actcttttta atatatcgtt tgtttccgct atgacaaaat
    10081 ccagctttat tcaaattttc tcgacacagg ggacaagcgg atgagtattt tcataaacaa
    10141 tgaactgact gtaatagata ggagaacgaa tagtgtctcc aacaatccaa aaacataata
    10201 attcaaaaag acgataagac tattggtaag taaaagtcct tgcggcattg gaagccgaaa
    10261 aaatttgtgg aaaataatcg gcggtatcgt tataccgcca tttgaggccc cgactgtgta
    10321 caatgtgtaa accccaagac tgaacaaaag acttcctgaa atcagtgaaa agaaatgtga
    10381 ggagatcatt tccacttttg gaacaatctc taaaacaaca ggaaaaagca gacttcccgc
    10441 tacagtctta aaaaatactg attttcctaa taggacagcg gctagaagca gcatcataag
    10501 attgatgctc caaacagtaa ctgagcgttg gatagaaaag gccgtttcaa ctaaatgtcc
    10561 gatggcgccc actccggcgg aagctatatc gtgagggagc aagaaccaat tcaatccaac
    10621 tgtgataaag aaaagaccta aaaatataac gggtagtttt tttatttctt gtgtcattga
    10681 tatctcctat aaatgttagc cataaaaggc tgtatatttc tttactttac caaaaaaacg
    10741 aaaccgaatt caaaaaaaca attgttctta taaaagttat ataaaatcga ttgccagttt
    10801 ctgttttacc aattaaaatg atagtaatgt tttgtagaaa gttggaatct gaccgatgga
    10861 taatcaaaga aaaccagcat tagatcaatt aacaccgcaa gaaaaaatgg cacgaggctc
    10921 tgcttggatg accgccagta atatcatttc aagattgcta ggcgctgtct atatcattcc
    10981 ctggtatgct tggatgggcg aaaatgctaa ggctgccaat ggattattca atatgggata
    11041 caatatatat gcattgtttc tactgatctc tacagcagga atacctgctg cgattgcaaa
    11101 acaaactgca cgctataatt cattgaatga atatggcact tcacgacgat tatttatccg
    11161 tgcattacag atgatgggcg ggttaggcct gttgtttgct ttgttcatgt acatcgcctc
    11221 tccttggcta gctcatgctt ctggtggcgg agaagaactg attccaacga tgcgttcttt
    11281 gagtatcgct gtattggttt ttccttgtat gagtgtgata cgagggtatt tgcaaggcaa
    11341 tcaagaaatg atgccttatg cgctgtcgca gattgtagaa caggtggcac gtatttttta
    11401 tatgctattg gctacattca tcatcatgaa agtcatggat ggtgactatg tgacggcggt
    11461 cacgcaatca actttcgcgg catttatcgg tatgctagca agtattcttg tccttttgta
    11521 ttttttgaag aaacatcaag cgtatacatc ggcgtttatt cactacagcg aaaacaaaat
    11581 aaatattgca acgaaggaat tgttgttaga taccgttaaa gaagcgattc cgtttattat
    11641 tgtgggttca ggggtaacga tcttcaaatt agtcgatcag ttcactttta tgaagatcat
    11701 gtctgcatcc accgagtatt ccaatgcaca gttactggat cttttttcga tttttagtgc
    11761 caatccagat aagttgacga tggtagttat tgcattggca acatcgatcg cgtctactgg
    11821 gttaccgttg attacagaag ctgttacgtt gaaagatcga gtaggtctgg caaaattgac
    11881 aagcggaaat ttacagttgt tttcattttt tatgtttccg gcgacatttg gtgttatgtt
    11941 acttgcctat ccgctaaata cattatttta tacaccggat tctctaggaa gtaatgtact
    12001 gatccaagca agttttgtag gactcttcct aggactatat atgctagttt ccaacatgct
    12061 gcaaggaatg tttgaaaaca aggcggcaat taattatttg gttattgggt ttctagtgaa
    12121 actccttctt caatacccag ccattcgttt atttgaagta tacggccctt tattggcgac
    12181 aatgatcgga tttgctgtgt cttgtacctt gatcttaaag agaatccatg aagtagcccg
    12241 ctttaaacca agttttgtct ggcgcaggac attgctgatc tttattctta ccttgattat
    12301 gcttttggct tcgtggatca caaagatgat ttttggtgct tttttaaacg aagatagcaa
    12361 attccagtct ctattgttga ttcttgtagt agcaggtata ggggctttag tgtatggtta
    12421 tttagctttg aaaatccgct tggcagaaag actactagga ccaggaatag cgcgtttacg
    12481 acataaatta aaaataaaat aagtgaatca gaagagctag attgatgctc ttttgaaatg
    12541 ggggaaacgc atgcgtttag ataagttttt agcagatatg ggacttggca gccgcaaaga
    12601 agtcaaagga ttaatcaaaa aaggaacgat cgaagtcaat ggacaagtgg tgaaaagcga
    12661 taaagcgcaa gtaaatgaat tcgaagatgc ggttacttat ttaggagaaa tcattacgta
    12721 tcaaaaagat ttttactaca ttttgcataa acctgcagga gtcatttctg ctactcagga
    12781 taatcatgat caaaccgtaa tcgatttatt ggaagaccag gattatcggg aagatctttt
    12841 tccagtcggc cgattagata aagacacaga gggcctcttg attttaacga atgacggggt
    12901 gtttgcccat cagttactat cacctaaaaa acatgtggag aaagaatatc tagctgaggt
    12961 tagcggtata atgacagaag aagatatccg tttatttgca gaaggactga ttattgatgg
    13021 cgatgagcag acattgccgg caaaactcca aattgatcaa gtagatcaag agacagaaac
    13081 ttcaattatc cgtctgattt tgcatgaagg aaagtttcac caagttaagc gaatggtcaa
    13141 agcagtagga aaagaagtct tatatttaaa aagaatccgt atgggaaaat tttggctgcc
    13201 taaagatttg aataaagggc aatatcgacc catgaccaaa gaagagctag aacaagtgag
    13261 agaaaaataa ttgaatggat agctaaaaga ataagcagag tagttgtagc gccatctgaa
    13321 aagcagactt gtccgtctga ctttcggatg gcttttttgt gaaatttcgg cttatttccg
    13381 tagaagctac ttctggaaca ccgtttgttc ggaataaggg actgtgacaa gacttttgtt
    13441 acagtcccca ttgttccttg tgactcagag ttagatagta aggacacgat ctccctaata
    13501 ctcaggccaa aacgctgaat tcacaacctc tgataaacgg tgttcaatca gctgaccctc
    13561 ggtattagct caaaaaacgg caaaatatga ggaactattt tcccgttttt ttctctaata
    13621 ctcaggccaa aacgctgatt tcacaacctc tcctatactt tgtttcattt tccctatact
    13681 ttgtttattt attctcatta ttgaaagcgc tataattacg ttgcgaatga atcgaaaggg
    13741 gtttttagat gaaaaaacga aaggggttca atcatttttt tatgaatcta tggcgttata
    13801 aagcacttgt cctgatggct gtaccgggta tgctttggat gatttttttc ttttacatac
    13861 ctgtaatggc gaatgtcgta gcattcaaaa attttcatat ttcagcagat gggttcttgg
    13921 ctagcttgag ggaaagtcct tgggtgggtt gggaaaattt caaattttta ttttcttcgg
    13981 atcaagcatt tctcatcact aaaaatacaa ttttatataa tgtagcattt atccttttga
    14041 atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat aaacgactcg
    14101 ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc attatcagtt
    14161 actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg atcactggac
    14221 atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt atcttggtgt
    14281 tcattggtac atggaaaggg attggttata acagcattat ttactttgca tctgttatgg
    14341 gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa tggcagcaga
    14401 tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg attttagccg
    14461 taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg aattccggtg
    14521 cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg acatccacag
    14581 gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc tttgtcttgt
    14641 tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta ttctaaggag
    14701 gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca ataagacggc
    14761 taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt tgccatttat
    14821 atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg ggtacagttt
    14881 ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga tgcaggataa
    14941 aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac tgatcaatag
    15001 cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat tccgtcgttt
    15061 ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg ccaattatct
    15121 tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct tgccaatggc
    15181 tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg tctctgattc
    15241 gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg ttcagatcgt
    15301 tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat tgggttactg
    15361 gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc cactacagta
    15421 tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag gagcaggaag
    15481 ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg cgatcgtagt
    15541 catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt ttgtcaaagg
    15601 tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata aaattaggag
    15661 gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg ttgttgttgg
    15721 gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct agcaattcgg
    15781 acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa ttgatggaag
    15841 tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat tacattggct
    15901 ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat tatgatatcg
    15961 cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat ttgactgaat
    16021 tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat attaaaggaa
    16081 atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt gctcaacaag
    16141 tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat ggaatccaat
    16201 cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca aacacggcag
    16261 cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct ttagggaata
    16321 cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac caatacgata
    16381 atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa ggactcattc
    16441 caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact tggctaatgc
    16501 gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat gcagcaaaac
    16561 aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa gcacaaatgg
    16621 caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa cttctagggc
    16681 tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag ggagaagcat
    16741 gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat cagccaaata
    16801 cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa gagtctatta
    16861 cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact tcaccgattt
    16921 taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt tctaatgtca
    16981 tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa acattaccga
    17041 aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa atgcaaaaac
    17101 aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg tgacaaaaga
    17161 cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct cggtattaag
    17221 ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac tactcgaaac
    17281 taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc ctcggtattc
    17341 gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta ataatcaggt
    17401 caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact taatgtttgg
    17461 aggagaagat atgttaggga aggttttgga agagttattt atccggatct gggtcattat
    17521 aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct ttggattagg
    17581 tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt ataaagaaat
    17641 cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca atctgctatt
    17701 tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat ctgttcaaat
    17761 caaagggctc atttttttag ctattgattt tattttactt tttgccttgc tctgcctatt
    17821 tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg ttcctaatct
    17881 gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa aaataattat
    17941 tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc tgtttggtgt
    18001 cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg aagaattaga
    18061 taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga accttactaa
    18121 atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact gctaccgtta
    18181 tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg acaagtgatg
    18241 ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg atcatcaaaa
    18301 accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta acaaaaccca
    18361 tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa ttagtttctt
    18421 tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg atatatatcg
    18481 tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt ggggaaataa
    18541 aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc acacaaggag
    18601 gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat atgattttag
    18661 aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg acaaatcgac
    18721 tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa gaaatgatca
    18781 tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa aaaaacaatt
    18841 ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta gataaaaaaa
    18901 gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc attattttag
    18961 acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag caagtatcta
    19021 tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg atcgacataa
    19081 caaacacgga atatgaactg agggaacttt caatcggtat caacgaaatg ttggatagca
    19141 tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac gcacatatgc
    19201 gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa tatatccgaa
    19261 tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg ttttccgctt
    19321 tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa ctggactttt
    19381 gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc tatcatttta
    19441 tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag ccattagttg
    19501 aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta agtgtcaaag
    19561 tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa gggatgactg
    19621 aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa cttcacggtt
    19681 ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc agttattata
    19741 tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc aaggagggat
    19801 gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg ggttaaaaaa
    19861 gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga atgccaaaga
    19921 aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg tgacaatgcc
    19981 agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata attttgaatt
    20041 tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc aattaggcgc
    20101 cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc tcaagaaaat
    20161 caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct atcaagaact
    20221 gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag aaataatagg
    20281 acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa ctcatgatac
    20341 tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac gaaattacgg
    20401 agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct tctgttgttt
    20461 tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag agacagattc
    20521 tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt tgcatcaatt
    20581 ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa aagatcaaac
    20641 catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag tcgctcaaaa
    20701 aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg aagatatacg
    20761 atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc tggaagatga
    20821 ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag aacttcagca
    20881 gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg acaatgtgga
    20941 aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca aagaagtgtc
    21001 agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag aaacgaaaaa
    21061 aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac tgcttttgca
    21121 cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca ccacgtattt
    21181 ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag aaaacagtgg
    21241 agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg tctagaaatc
    21301 taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc tctcgataaa
    21361 cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtggaaagcc
    21421 attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga cctctccact
    21481 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatggcaaa atatgaggaa
    21541 ctattttgcc atttgtctct ctaatactca ggtcagaacg ctgatttcac aacctctcaa
    21601 taaacggtgt tcaaaagctt gcatctgcgg taataagacc aaccaaacga aaaatatgag
    21661 gatctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
    21721 tgttaattat agtttgataa ggaaaaaggc aaatcttttg cttgatattt cttagggtga
    21781 gggatgatcc ctaattggta agagatcgtg ccgcctttca gcaaatcttg atgggtaaaa
    21841 tataatttcg tatattctct gtccatccgt ttgattttat ggataaattg ttgctgcggc
    21901 cgattaggcg ttgcttcaat cgtcaattcc tttccatttg acaaaagcaa aacagccttg
    21961 tcaattaatg gcataccgat aacatattca ccggttccag gcgtgacagg ataaaatccc
    22021 agactattga agatatacca agcggccata cttccattgt cctcatctcc agggtaaccc
    22081 attggagaat cgtcgaaggt ctgagtcatc aactgtttaa gcagtggctg tgccatttca
    22141 ggttgtccta tataactgaa caagtaagga tagtgaaaac ttggctggtt tgaaatagct
    22201 aattgtccaa attctatcgc ggccatctca ctcatttcgt ggatctcaaa accatatcct
    22261 cctgggtcaa acgttggggg ctgattgcac agctcaatga gtttttcttt atatctctca
    22321 ctgccaccat attttccgat cagtcctgaa aaatcgtgat aaacagcaaa gctgctttgc
    22381 caagcacttc cttctgcata atcttgtccc caacgattag ctgaaaatgg ttctctgaac
    22441 ttgccatctt tatcttttga acgcataaat cctgactcat agtcaaagat attttgatag
    22501 ttcaatgatt gctgctgata tttcttcgtc atttcctctt cttctagtac cttggcaact
    22561 tggctaatac aaaaatcgct ataagcatag tctaatgtgt gattgactga ttcgtggtag
    22621 tgattaggaa catagccata tgtcaagtaa tcttctgttc cttggcgtcc ataatttgga
    22681 tgtttgcttt gaaccgtagc acctttttcc attgccgtta gaaattcagg catcaagtct
    22741 cgtcggatat cttttacagc agcatctgca atgaccgcat cgatcaatgt cccaggcata
    22801 agtccacgtt catcaggaga cagccatttt ggcaaatagc cactttctct atagctgttc
    22861 aaaaaacctt ccagcatttc ttcgtatttt tctacagcaa tcaaagaata aagaggataa
    22921 accgttttat acgtatccca aaacccatta tttgtataga gaacaccagg ttttatagat
    22981 cgactagtcg tatcatagtg gattttttgc ccactaggat caatttcata aaatgtttgt
    23041 ggaaataaaa acacccgata taaattatga taaaacgtcc gcaattgttg ttgatcatgg
    23101 tgtgtgagct gtatacgatc aaaataggac tgccattgtt cttgactgtg agcaaggtaa
    23161 gtctctttgc tttggttttg ttctagttcc aggttataag ctgcttgttc cgagctgata
    23221 aaagatgtcc caaatcggat agtttgtttt tttgtctgtt caaaatacaa cgggatcgag
    23281 tcgctatttt tgttggaaac agctaactca tcaattgtta aaggatattc aaatctcaat
    23341 ataaaataaa aagaaaaatc aggatcttct gaaccagcaa aatgtgtgat ttttccttcc
    23401 actgtgtaag gatctataac aaagagctga tggcgtcccg gaaaggataa aagtaaaccg
    23461 ctgtcatttt tgctgtaatc aatagataag attccgccat acatgctagg tatcaatgta
    23521 gaacagatgc cataacgtaa ttgacagatc gacagatgag tcgggcaaaa tatactttct
    23581 tctgggcggt aagaactttg agcatggaaa agggtgttct cctgtagttt tccattgatc
    23641 ggcgtaaaca gcatatgact gaaatcaccc atccaaggac ttggttgatg ggtaagacgg
    23701 tagccttgaa aaacgcggtc atcaggatga aaccaccagc tgccgttttg atccgtagtt
    23761 tgcggtgcaa aataattcat cccaaaagga acgccagtat aaggctggca gttcccatta
    23821 gagaaatttg gctgattcgc tgttgcatgt ctagtatcta tttgaccgat attcatttta
    23881 tttctccttt gttgattgtc ttttgatgaa tgaaactgga aatttactat cggatggtcg
    23941 atcttttgtc tctaaccaag tgaccaaagc atctcccgca agcttaccta actgttcaaa
    24001 atcttgtgcg atggttgtca gtggcgggtc aacaagagct gctgcttgga tatcatcaaa
    24061 tccgataatt gaaaaatctt cagggatatg ataaccatgc tgtctcaaga gattcatggt
    24121 ctggatagcg acaacatcgt tttcacaaac aaaagcagtt acttcatact tagtgatata
    24181 agaaagcaat tcatctaatg tcgcgctttt gtcatccagt gaagtgtgga aagtcaaacc
    24241 agctttgtcc aatgcttcaa ggtaaccaag gtaacgctgt cgaacggatt gtggatgagt
    24301 ctgcgtacca aacaaataag cgatttttga atgtccctgt tcgatcaaat aagaggcagc
    24361 aagcgcaccg ccttggaaat tatcggaaag aacggtagga aaaggtaact catagatttt
    24421 tttatctaat atgacgacag ggaattcttt caatgaaagc tcaaaaagta agtcaagatg
    24481 ttgttctgta tcaagagcat aataaatcaa tccgtcaaat tcattgatta gctcttttgc
    24541 tgttttattt tgtagaaagt caagcgtcgt catcatgaca tcaatctgtt tatcttgcat
    24601 cacagggttc aatcccttgc taaaatcacc aacagacaaa tcagcgacat atggaagaag
    24661 aaacagaatc cgtcgtgttt ttgtaggaga agcaagaatc tgttccggag aagcggattt
    24721 aacgaaactg cctttcccgc gaacgcgata aataagtcct aattgctcta attcagtcaa
    24781 tgcacgtttg gaagtgattc tgcttacttt ataggtacgt gaaagttctt tttcagttgg
    24841 tagttggctg tctacaggta attcaccagt taaaatagcc gtttgcaaat cattaaatat
    24901 tgtctggtac aatggttgag tcatataaac acctcttaat gatatatcat attcaaataa
    24961 taccataatg gatgaaaaag taaataaaaa gatatttttt gaaagcgtag acataattgg
    25021 ctgtatgaag tataatcttt aaagatatat caaatagaaa gaaggtttta ttagtggttt
    25081 ataaagatat accaaaatcg gtgcagaaat ttatggaaga tatcacacaa aaatgtgggg
    25141 atatccatgc ggactgggca aagaatttta cagcagcatt tgccaatact ctgttgacaa
    25201 cagtcaaaaa gcaaaatgac ggaaccacct ttttattgac gggagacatt ccagcgatgt
    25261 ggttaagaga ctctactgca caagttcgtc cttatttagt gattgcaaaa gaagatgagg
    25321 aattagctga catgatcagc gggttagtca aaaagcaatt tttttatatc aatatcgatc
    25381 catatgcgaa tgccttcaac gaagaagcta acggtgctgg ccaccaaaca gatcatacaa
    25441 aaatgaacga ttggatctgg gaaagaaaat atgaaattga ttctttatgc tacccaatac
    25501 agttggccta tcttctttac aaaaatacag gaaaaactga tcaattcaat caatctttta
    25561 ttgaaggtat caaaaaaatc ttggacgtgt tcaaaacaga gcaagatcat gaacactctc
    25621 cttatacatt tgtacgagac acgacaagaa aagaagatac gctgatcaat gacgggcgag
    25681 gtgcaaaaac agcttttaca ggaatgacat ggtctggttt ccgtcctagc gatgatgctt
    25741 gccgttatgg atatctggta ccatccaata tgtttgcggt ggtcgtgtta ggttatgtgg
    25801 aagaaatttt tcaaacgata ctcgatgata aagaaattgt acagcaaaca cagaaattga
    25861 agaaagagat ccaagagggg attcagaatc acggacgaac ggttaatcaa aaagggcaaa
    25921 cgatttatgc atatgaagta gatggaatgg gaaacgccac agtcatggat gacagtaatg
    25981 tgcccaatct gatcgccgca ccttacttgg gatattgttc tgaacaagag gaagattatc
    26041 tgcaaacaag aagaacattg ttgagtaaag aaaatccata tttctatgaa ggaaagtacg
    26101 caaaaggaat tggatcctcc catacgccag aaaaatacgt ttggccgatt gcattagcta
    26161 tggaaggaat gacaacgtca gacaaatctg aaaaagagcg gatattgaat ttacttgtag
    26221 caactgacgc aggaacacac cttatgcacg aaggattcga tgtgaacaat cctgaaaatt
    26281 atacgagaga atggttttct tgggcaaata tgatgttttg cgaattagtc atggattact
    26341 ttgatattag agtagaaaag taggaggtat cgtaaatgaa aaagaaagta tatatcattt
    26401 ctcatagcca ctgggatcgt gaatggtaca tggcttacga acagcatcac atgcgactga
    26461 ttgaattgat agacgatcta ctagagctat ttgaagttga tccgtccttc aacagttttc
    26521 atttggatgg gcaaacgatt attttagatg actatcttca agtaaggcca gaaaaaagac
    26581 aggcaatcca acaagcaatc aatgaaggaa aattgagaat tggaccattt tatatcttgc
    26641 aagatgattt tcttatcagc tcggaatcga acgtgagaaa tatgctgatt ggcatggaag
    26701 aaagccgtaa atggggaacc cctgtcatgt taggctattt tcccgataca tttggaaata
    26761 tgggacagac gcctcaatta atgaaacaag ctggtatttc agcagcagcg tttggccgtg
    26821 gtgtaaaacc aattggcttt gataatcaag ttttggaagc agaaaactat tcttcccaat
    26881 actcagagat gtggtggaaa ggccctgatc aaacagcaat ctttggtctg ctatttgcca
    26941 attggtatag caacgggaat gagattccag tagaaaaaga agcagctctt gccttttgga
    27001 agcaaaaatt agcggatgca gaacaatatg cctctacgaa ccacttattg atgatgaatg
    27061 gtgttgatca tcaaccggta caaaaagata tatcaaaagc aatccatctc gcaaatgaac
    27121 tgtttccaga ttacgagttt attcattcta attttactga ctatctggaa gcagttcaaa
    27181 aagatgtgcc agaagactta ggctcagtag aaggagaatt gacaagtcaa gaaacggatg
    27241 gctggtatac tttagccaat acagcatctg ctcgggttta cttaaaacaa tggaatacca
    27301 aggtccagcg acagctagaa aatattacag aaccacttgc gacgatggct tatgaagtta
    27361 gtggaaacta tccccatgat caattagatt atgcttggaa gacattaatg caaaatcatc
    27421 ctcacgacag tatttgcggt tgttcagtcg attctgtcca tcgggaaatg atacctcgtt
    27481 ttgaaaaagc agatgaagtt ggaaagtatc ttgcacaaga ttccttagaa cagctgaccg
    27541 cagcaatcga tacgaccgga ttcccaaaag acagctttcc atttgtcatt gtaaatactg
    27601 caggaatgga taaaactggt gaagcagaaa taaccatcga attagaaaga aaaagattcg
    27661 ctgaaggaat acctgaacaa ctatatcaag agttagaaaa tttgcctaaa cgtaagtatc
    27721 atgtagaaac aaaaagtggg gccactatac cagctatttt atctgaagaa actgtccaat
    27781 ttggttatga tttgccgaaa gaccgtttcc gtgtgccata tatggcaaga atgatcaaag
    27841 tgactttacc attagaaaac atgccagcat tttcttggga aacattcgcg cttgtagaag
    27901 gggaagcgaa agcagaagaa aaggaaacaa tgattcatca gagtggacga atcatcgaaa
    27961 acggccctct acatttgacg attgaaaaaa atggtaccat tacaatggaa gatcgcaaaa
    28021 acaaacgaaa gttgaataat ctgcatattt ttgaagatat cggcgatata ggaaatgagt
    28081 atatctttaa acaacctttc tgtgatcagc caatcctatc cagtaacaaa gaaaactctg
    28141 aagtaaaagt attagtagat acaccggaaa tagcaaaaat aagtattcta caggaaatgg
    28201 aaatccctgt ttctgcagac gagcgtttag aaaaagaaca gcagatggtt gtggaatttc
    28261 gttatcgaaa agcagaacga tccaaagaaa aacgaatcct tcaaataaaa acaatcatga
    28321 caatcagaaa agatagtaaa aaaatcgact ttgaaacgac tctcgacaat cagatgaaag
    28381 atcatcgctt acgtgtcttg tttcctacaa agttacatgt ggaacatcac gaagcagata
    28441 gtatttttga agtggtgaag cgcccaaacc atgtgtctaa aagttgggaa aaccccacga
    28501 atccgcaaca tcagcaagca tttgtcaata ttcatgatga agagtacggt gtaacagtcg
    28561 gaaactttgg tttgaatgaa tatgaagtaa cagaagacgg acaaatcgca gtgacgctac
    28621 ttcgcagtgt gggagaacta ggcgattggg gatattttcc aacaccagaa gcacagtgtc
    28681 taggtgagca tcggttcaat tacagcattg aactacatgg accagaagaa aaattctcta
    28741 cttatttgca tgcatacgca gcacagattc ctttttccac tcaacaaatc aaacatcatg
    28801 aaggaacatt gatttcaaaa caacaatatt taacgataaa aagtgaaacg tttgcaatta
    28861 cagctttgaa acgcagcaag tttagtgata aagtagtagt aagaggtttc aatatgtcta
    28921 gtcatttaga aaaattggaa ataactaaag acaatggaaa aactgtcata ttgaatctgc
    28981 ttgaagaacc aacgaagcaa gcggttgtcc caataatcca accttatgag attcggacaa
    29041 tcggattcga ggaggaaaac gaatgtatgg cggaatagaa gcaggcggaa caaaatttgt
    29101 gtgtgcggtt tcagatggtg gagaaatttt agaaaaatta agtatcccta ccacaacacc
    29161 agaagaaaca ttggatcagg tatttgaatt ttttgatcgt tatgaattga agggaatcgg
    29221 gattggctca ttcggcccga ttggtatcga caaaacaaaa gataattacg gatacgtttt
    29281 ggcaactccc aaaaaagggt gggcacaatt cgattttctt ggcacaatca aggaacgcta
    29341 caatattcct gctgcttgga caacggatgt caatgctgct gcatatgggg aattgaagca
    29401 aggagcagca caaggaaaaa atagttgtat ttaccttacc gtgggaacag gaatcggtgc
    29461 aggtgtcgtg atcaatgaag aaattttttc agggatcgca catcctgaaa tgggacatat
    29521 ttgggtgaaa cgccatccag atgaccatta tgaaggtact tgtccttatc ataaagattg
    29581 tttagaagga ttagcagcag gaccgtctat tgaggcacgc acagggatca aagggcaaga
    29641 tctgccgcaa gatcatcctg tttgggctat tcaggcttat tatattgctc aagcactgat
    29701 caactatacg ttgacgttag cacctgaaaa gattatttta ggcggtgggg taatgaatca
    29761 ggatcatctc cttcaaaaaa tccgtcagca atttgttgaa ttgatgggtg gttatatgga
    29821 aacacctcaa gtaagtgaat atatcgttcg ttggggactg ccgaatgaaa gtgggattat
    29881 cggtagtttg ctgttagcag aacacgaaca tcaaaagtaa gcagacaaat tacacaaaaa
    29941 gaacagagtc ttatcattat ctgctgataa gactctgttc ttttttattt ctattcccat
    30001 aatccaggta aagctttacc gactattttt tcttgagtaa atggatggat catgtgcagt
    30061 tctaatgcat ggagcatcag tcgttcagct gttctatttt ggtagagagg gtctccaacg
    30121 atcggatgtc ccttatgagc taaatgaact ctgatctggt gtgtacgtcc ggtttccaaa
    30181 acacaataga cttgtgtttg ttcagaaagt actttgttga cagtgacatg tgtcaaggcg
    30241 ctctgaccat ttttatcatc tacgaccctt tttctccgat catggcgatc tcgtccaatt
    30301 ttattggtga tggtcatatc tttggcaatt tctccccata cgatggcttg atatctccga
    30361 tagatcattt tttgttccaa cattcttcca agtatcggca agacaaatgg attttttgca
    30421 aataaaacgg caccgcttgt ttctttgtcc aagcggtgga caacgtaagg tcgttgctgt
    30481 ttagtgtcga gataagcagc taaatgattg agtaaggtac tgttttctgt cggttcattt
    30541 ggatgagtct tcattcctgc aggcttatcc actataatca aatgctcatc ttcatataaa
    30601 acacggatgt gcttcgcatc tcctaattga acttcttgat aattataatc tgtttcttcg
    30661 aataacaatg tgatctggtc attgcccttt acttcatgat ggaacaaagc aggttgttga
    30721 ttgatccaaa cgtttttacg tatacgcaaa aagtgtcgaa cctttctagg aactaaccat
    30781 tccttttcaa gcaagtcgcg aattgttgtt tcaggatggt tcataggtaa agtgatcgtg
    30841 attttcacaa gtgtactcct ttcagtgcaa ttgcagacta attgtaccat ttttaaaaca
    30901 tatttaagaa aaaacttaca tttttctgtt cattcttatg ttactctact tgcactaacg
    30961 taaaaaaaca tattgaacta caacttaagc aggaagaaaa cactctttca ctatggtaaa
    31021 ataagacgat tgaatcaaat agacaggaga agaagcaaat ggattttcaa agcattttag
    31081 aaaagataaa agctgctttg atcagttttt ggcgttggat cagaccatat ctcggacaat
    31141 tccatcgatg gcgaaagcgc atctggaaaa aatatcatgt gaacaaattg attttgctga
    31201 ttggtttgat ctgtgtctta gtgacaagta tctacctctt tattttagca aaacaagcaa
    31261 atgtcgaaac gttaaaatca ggattaagcc aatcgacagt catttatgat aagaaaaacg
    31321 aagaagctgg cacactgtac gcccaaaaag gaacatatgt agaattaaac gcgatttctc
    31381 cctatatcca agatgcagta atctcgacag aagataggaa tttttacgag catcacgggt
    31441 ttgatatcaa agggattgcc cgtgcagcag tccgaatggt cttgtctggc ggaacatcag
    31501 ggggcggagg aagtacgatc actcagcagc ttgccaaaaa tgcgtatctg acattagacc
    31561 aaacgtttga tcgaaaagcg aaggaattat ttttggcaat cgagatcgaa aagaagtaca
    31621 gcaaagaaga aatcttgacc atgtatctta acaatgccta ttttggcaac ggggtatggg
    31681 gtgtccaaga cgcttccaga aagtattttg gagtagatgc aacgaatgtt acgctgtctg
    31741 aggctgctac tttggcagga atgctgaaag gtcctggtat ttataatccg atcgatcacc
    31801 caaaaaatgc gaatgatcgg aaaaacacag tactaagtgt aatggaagaa aacggaaaaa
    31861 tcacaaaaga acaagaacaa gcagaaagcc agacagatat cagcgcgtat cttaatgaca
    31921 catacgataa ttcagattct ggttatcgtt atccttatta ttttgatgca gtgatcgatg
    31981 aagcagttaa cgagtatggt cttgatgaag aagatgtcat gaataaaggc tataaaatct
    32041 atacagcttt agatcaggat tatcaaaagc aattagaagc tacttatcaa aacagtgcat
    32101 tattcccagc aaatgcttca gatggtgcca tggtccaatc tgcttcagta gctttagatc
    32161 cgaatacagg tggtgtacaa gcactagtag gacgaagagg agatcatgtc tttagaggat
    32221 tcagctttgc tacacagatg cgtcgttcac caggatcaac gatcaaacca ttaagcgtat
    32281 atacaccagc actggaagct ggatacaagc cttccagtat cttgaaagac gaaccgcaaa
    32341 gttattacga tgcgcataat tttgacggga cataccaagg agaagtaccg atgtatcaag
    32401 ctgttgcaca aagtttgaat ttgcctgctg tatggctttt gcatgagatc ggtttacaaa
    32461 aaggatatga taaagcagaa gaattcggcc tgcctttagc aaaatctgat aagtattatg
    32521 gcttagccct tggtggactg gaaaaaggag tatctccatt gatcatggcg ggtgcttaca
    32581 gtgcatttgc caatgatggt cagatgtata cgccgcatct tatcacgaaa atcgttgatt
    32641 cgactggagc ggtgatcgtc gataacacaa acaaaaaacc aaaacaggtc atatcaaagg
    32701 aaacagccga tgaaatgacc agcatgcttc ttggtacatt ttcaaatgga acagcggctg
    32761 ctgcgaatcc tgcaggttat acgatagcag ggaaaacagg aaccacagaa actaatttcg
    32821 atgccacaaa agcaaatgac caatggatga tcggttacac gccagacgta gtgatttcga
    32881 catggatggg ttacgaagaa tccagcaagc tgcactattt ggaagggaca agtggaaatg
    32941 ttgtcgggaa agtcttcaaa tcagcggcag aaggcatcct tccgtatact tcaaaaacag
    33001 gatttagtgt agcagatgcc tatgccacag gaggacaagt ggtagctgca gatcaagttg
    33061 gaaacacagg aaattctaat ggagagtctg gaaattggca agataacttg aatcaatatg
    33121 gtgaacaagc aaaagaaggg ctgaaaaact tcggcgatat ggtgaaagaa ggcgttcagg
    33181 gaattggtga agcggctaaa gacctctggc ggaaatatca aggagagtag cccctttgaa
    33241 aacgaataac cccatgttac aatagagagt aactactaaa agaaaagagg gcataacatg
    33301 tcaaacatct acgatagcgc gaatcaaatc gaacgtgaga tccgtgaatt accagaattc
    33361 aaagcgttga aagaagcgta cgagaaagta caagcaaacg aagaagcaca caaactgttc
    33421 aaagacttcc aagcgatgca aatcgaatta caagaaaaac aaatgagtgg acaagagttc
    33481 agcgacgaag atgcaatgaa agcacaagaa atggctgtga aaatccaatc agaagaagta
    33541 atcaatgact tgatgcaaaa agagcaaggc ttcagtacaa tcatcaatga cttgaaccgt
    33601 atcatcatga caccagttcg cgacctttac agcgaataaa actattttat gaaacaacag
    33661 agggtgtgaa agagacgttc ggccttgagc gatgacaaaa ttgcttctta ttgccgatag
    33721 gctgtctctt gaacaccatt taccaagagg tcgtgagcct accgtttaac tctgagttac
    33781 aaggaacaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact tgtgaccgag
    33841 gagttggcag gagaacaccg tttattcaga aataggagct acgacaaaag tcttgtcata
    33901 gctcctattt tttgtcattc atttttcaag cggtagaaat agcctttttg agctaacagt
    33961 ttttcgtaag tacctatttc aacgattttt ccttcatcga tcacaacgat tttatctgcc
    34021 tctttgatcg ttgaaagccg atgagccaca ataaaagtag tgggtgtttc tgtcaggtaa
    34081 ttcagggctt gctggatttt tttctctgac tggttatcta aagctgaggt tgcttcatct
    34141 agcaaaatga tttttggctg acgtatcaac gctcgtgcaa tggagataca ttgcttttgg
    34201 ccaccagaca gcttgttgcc atgttcgcct actaaggtat ccagaccatc aggaagctgg
    34261 tcaatcagtg aagaaagttg cgcagcttcg atgacttcat ctaaacgttc ttttgatacg
    34321 tttttcaagc cataagtgat gttctctttg atcgaagcgg agaacaacaa agtggtttga
    34381 ggaacaacgg ctaaataatt tcgcacactt cgcatatcca gttcatccaa aggctgatca
    34441 tctagaaaaa tttttcctga agtcggctgg ataaatccaa tcaataagtt aaggagtgta
    34501 gattttccag agccagatgg cccaacaaaa gcaattgttt ctccagcttt cacctctaga
    34561 gaaaaatgat cgataatcgg tttgagtgag tccggataaa gaaaagaaac atcctcaaaa
    34621 cgaaaatttc catccaaatg ttggattttt ttcttaccgt gataagtttc tacatcgcca
    34681 gcaatcaaca gttcgccaat agaagtgata gattccatgc cttttgaaat cgtaggaatc
    34741 agcgtgatca ttccagaaac ggaattgacg atcgtcgtga aatacgtctg atacatcacg
    34801 acatcgccgg gttggatgcg cccttgtaaa gctaatatcc cagtgaaaat caaacaaatc
    34861 agctgaaata tctgaaaact agcccagctg actgatccga aaacagattg caggatatct
    34921 aagtggaatc ctttttcata gatttgagta aaatgcttat ttaagcgagt tgtttcgtgt
    34981 ttttctaacc catgagctcg agcaatcggt gctaattcga tcacttcgat cacttttgca
    35041 gaagtctctt ccatttcttg cctgaagtct gagttggact gtctgatcct tttacggaaa
    35101 aagagagtaa cgaatgcggc gagaggaaca gtcaataaga aaaacatgaa aacaacagga
    35161 cttttataaa gcgtgatacc taaagctacg aataaattga cagcgatatt taatccgctg
    35221 acaaatactt gagaggacaa cgtttggata gcttctacat cacggatgat ctttgactgg
    35281 attcgtccag attgcaattc tttttgataa ggaatagata gttcttggat tttcttgata
    35341 agggtcgctc ttagtccagc ttctacttga cggatcgaag tgctatggta ttttacatga
    35401 aaataattcg tcaatatatt ttgaccaatc agacaagcca tcaaaaaagc gttgaaataa
    35461 atggcctgca actgagaaga atctttcgat gaagctgcat tgattacatt ggcaatagcg
    35521 atcggcatta accaagatgg aagatgtttg ataataaaaa agagtcctga catggcaaaa
    35581 cgccaataat ttccctgata aagatgcagc aatgtctgaa aagcatttgc atgatatttt
    35641 tgataacttt tctgtattcg ttcctccata atcttctcct taacaatctt ggtaaataaa
    35701 ctgcaagtgt tctattgctt caggaacata aatctcagga gtagcttcaa gagtggcgta
    35761 cattagagga cgctcatatt tgatataatg cagtaccggg cggaaatcta aacagccctt
    35821 gccaacaggt accatgcgga tctttccatt ttccatgata tagtctttga gatgaagcgc
    35881 tgtaatataa ccatgtaaat tgtccaaggc acctcggatc actttttcct gatcagcatg
    35941 attttcttta tgcatcaaat tcgcacaatc taaaatgatt tttaaatttg gtgaagctac
    36001 ttcatcgatt aaacgtttag ctaactgata gttatagatt ggatgattga tccctgcttc
    36061 aacagcaaca gttacgccaa aattttctgc atgagcgact aattcaatga tagaatttct
    36121 ggcgatctga taagcttctt cagtaaaatt ttcttttgtg taaccgacag agacactacc
    36181 ggtttccgta gcaatcatct tggcttgata ggcagagcac agagcaattt gctgtttgaa
    36241 agttgccaat gcttgtgcac gaacttcttg atttttgctg gataggttga tatagcatcc
    36301 taaaatggaa agttcgatat tgtttttttg gaagtattca ccaaaatagg cagcactccc
    36361 aagtgtcatg tcagttaatt taagttgttc aggaaatgct ttttgtggtg caaattggat
    36421 atgggacaag tgaagctgtg atactttttg aacgatttct tcacgatttc gaacatctaa
    36481 atcgtgggca cggatgccta aatttaatgg aaacatctca aactcctttt tgtaaataat
    36541 tagcaagagg cagacgggat tcttttatca attcaacaat tattcgggca actttatcag
    36601 cgccttctgg actaaagtgg gtattgtctt ttagtccttc tgggtaattt ttacaagtat
    36661 caggcataag atgcaggtag aatttttttg tctcttcttt tgagaaggtc tggaacaatt
    36721 cttgtgatct tgagaacaca tctaaaagag gaatatggtg tttctcagca aatgcacgca
    36781 tcgcttttgg atagtctcca agtatatctg gattcaatgt tccgttttct aagtaatctc
    36841 gtcgcgtcac agatgtcagt aagaccattt ggacgttcgc tttttttgcg cattcaaaaa
    36901 atatctgcag attttttaaa tagctaccga agggaagcgt acctctttct tcttggattt
    36961 tctggtcgtt atgtccaaat tgtacaaata agaaatcatt cggctgaaac tgtgaagcta
    37021 gacgggctaa ccttccttcc tggataaatg atttggtgct gcgtccattc tttgcttggt
    37081 tattgatctg gatctcgggt gaaaaataag cagagatttt ttctccccaa cctgtctcgg
    37141 gacgcttgtt ttcatctttt tctgccgcag tagaatcacc ggcaatatgg atagtaggca
    37201 tgttatctct tctttcataa aggtttatag gcaatgaagc agaacagcaa gagaagaaga
    37261 cggagcaa
//
LOCUS       DAJSBC010000024        34519 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSBC010000024 DAJSBC010000000
VERSION     DAJSBC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.024"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            211..603
                     /locus_tag="ONT65_001224"
     CDS             211..603
                     /locus_tag="ONT65_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2328869.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            631..1971
                     /gene="glnA"
                     /locus_tag="ONT65_001225"
     CDS             631..1971
                     /gene="glnA"
                     /locus_tag="ONT65_001225"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR2328870.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="ONT65_001226"
     CDS             3164..4774
                     /locus_tag="ONT65_001226"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR2328871.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="ONT65_001227"
     CDS             5066..5935
                     /locus_tag="ONT65_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR2328872.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ONT65_001228"
     CDS             6100..7371
                     /locus_tag="ONT65_001228"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2328873.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ONT65_001229"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ONT65_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR2328874.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ONT65_001230"
     CDS             8762..9025
                     /locus_tag="ONT65_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR2328875.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ONT65_001231"
     CDS             9470..9919
                     /locus_tag="ONT65_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2328876.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ONT65_001232"
     CDS             9976..11394
                     /locus_tag="ONT65_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2328877.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ONT65_001233"
     CDS             11394..12254
                     /locus_tag="ONT65_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR2328878.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ONT65_001234"
     CDS             12428..13201
                     /locus_tag="ONT65_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2328879.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ONT65_001235"
     CDS             13348..13713
                     /locus_tag="ONT65_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR2328880.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ONT65_001236"
     CDS             complement(13861..15069)
                     /locus_tag="ONT65_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2328881.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ONT65_001237"
     CDS             complement(15088..15366)
                     /locus_tag="ONT65_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328882.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15520..16215
                     /locus_tag="ONT65_001238"
     CDS             15520..16215
                     /locus_tag="ONT65_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2328883.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16657..17454)
                     /locus_tag="ONT65_001239"
     CDS             complement(16657..17454)
                     /locus_tag="ONT65_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR2328884.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ONT65_001240"
     CDS             complement(17451..18635)
                     /locus_tag="ONT65_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2328885.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ONT65_001241"
     CDS             complement(18660..19907)
                     /locus_tag="ONT65_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2328886.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT65_001242"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT65_001242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR2328887.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ONT65_001243"
     CDS             21887..22201
                     /locus_tag="ONT65_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR2328888.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ONT65_001244"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ONT65_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR2328889.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="ONT65_001245"
     CDS             22886..23530
                     /locus_tag="ONT65_001245"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR2328890.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ONT65_001246"
     CDS             23545..23874
                     /locus_tag="ONT65_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR2328891.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ONT65_001247"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ONT65_001247"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR2328892.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ONT65_001248"
     CDS             24862..25686
                     /locus_tag="ONT65_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR2328893.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ONT65_001249"
     CDS             25679..26026
                     /locus_tag="ONT65_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR2328894.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT65_001250"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT65_001250"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR2328895.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ONT65_001251"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ONT65_001251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR2328896.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT65_001252"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT65_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR2328897.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT65_001253"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT65_001253"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR2328898.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31656..32450
                     /locus_tag="ONT65_001254"
     CDS             31656..32450
                     /locus_tag="ONT65_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328899.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ONT65_001255"
     CDS             32440..34482
                     /locus_tag="ONT65_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328900.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11099 a         6121 c         7249 g        10050 t
ORIGIN      
        1 ataaaaacac taataaagga tagtacagta taataaaaaa taaaaggtat ctggtacgaa
       61 aatcaactag ataccttttt tgtgttctta tttttttgtt attttataaa aaatagagat
      121 ctcatgttag aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta
      181 gccaacttat aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg
      241 tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc
      301 tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat
      361 tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga gggattgaat
      421 atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac
      481 aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa
      541 ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac
      601 tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa
      661 atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc
      721 ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat
      781 aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg
      841 tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa
      901 gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct
      961 cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat
     1021 ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg
     1081 accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc
     1141 cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat
     1201 gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt
     1261 gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac
     1321 gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg
     1381 tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt
     1441 gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt
     1501 tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat
     1561 gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta
     1621 tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca
     1681 gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt
     1741 gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta
     1801 ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca
     1861 ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc
     1921 agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat
     1981 accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga
     2041 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2101 accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag
     2161 caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt
     2221 gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt
     2281 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
     2341 ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata
     2401 atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca
     2461 ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt
     2521 tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg
     2581 tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata
     2641 tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga
     2701 aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta
     2761 gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat
     2821 ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt
     2881 ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat
     2941 aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac
     3001 ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa
     3061 gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggcatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgatt tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaat
//
LOCUS       DAJSBC010000025        32431 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSBC010000025 DAJSBC010000000
VERSION     DAJSBC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.025"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..310
                     /locus_tag="ONT65_001256"
     CDS             137..310
                     /locus_tag="ONT65_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328901.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="ONT65_001257"
     CDS             950..1423
                     /locus_tag="ONT65_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR2328902.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="ONT65_001258"
     CDS             1432..1659
                     /locus_tag="ONT65_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328903.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="ONT65_001259"
     CDS             2295..2666
                     /locus_tag="ONT65_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328904.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="ONT65_001260"
     CDS             complement(2922..3164)
                     /locus_tag="ONT65_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2328905.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="ONT65_001261"
     CDS             complement(3196..4098)
                     /locus_tag="ONT65_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2328906.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="ONT65_001262"
     CDS             complement(4111..4299)
                     /locus_tag="ONT65_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2328907.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ONT65_001263"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ONT65_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2328908.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="ONT65_001264"
     CDS             complement(4914..5807)
                     /locus_tag="ONT65_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2328909.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6807)
                     /locus_tag="ONT65_001265"
                     /pseudo
     CDS             complement(5885..6807)
                     /locus_tag="ONT65_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6856..7206)
                     /locus_tag="ONT65_001266"
     CDS             complement(6856..7206)
                     /locus_tag="ONT65_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2328910.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ONT65_001267"
     CDS             complement(7239..8141)
                     /locus_tag="ONT65_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2328911.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ONT65_001268"
     CDS             complement(8134..8991)
                     /locus_tag="ONT65_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2328912.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ONT65_001269"
     CDS             9325..10134
                     /locus_tag="ONT65_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2328913.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ONT65_001270"
     CDS             10174..10671
                     /locus_tag="ONT65_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2328914.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ONT65_001271"
     tRNA            complement(11056..11129)
                     /locus_tag="ONT65_001271"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="ONT65_001272"
     CDS             11316..11639
                     /locus_tag="ONT65_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328915.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="ONT65_001273"
     CDS             11803..12057
                     /locus_tag="ONT65_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328916.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="ONT65_001274"
     CDS             12127..12372
                     /locus_tag="ONT65_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2328917.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="ONT65_001275"
     CDS             12448..12813
                     /locus_tag="ONT65_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328918.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="ONT65_001276"
     CDS             complement(12874..13437)
                     /locus_tag="ONT65_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328919.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14005..14205)
                     /locus_tag="ONT65_001277"
     CDS             complement(14005..14205)
                     /locus_tag="ONT65_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2328920.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            14601..14750
                     /locus_tag="ONT65_001278"
     CDS             14601..14750
                     /locus_tag="ONT65_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328921.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            complement(14957..15670)
                     /locus_tag="ONT65_001279"
     CDS             complement(14957..15670)
                     /locus_tag="ONT65_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2328922.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="ONT65_001280"
     CDS             complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="ONT65_001280"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2328923.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16765..17208)
                     /locus_tag="ONT65_001281"
     CDS             complement(16765..17208)
                     /locus_tag="ONT65_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2328924.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17242..17595)
                     /locus_tag="ONT65_001282"
     CDS             complement(17242..17595)
                     /locus_tag="ONT65_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328925.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17707..18108)
                     /locus_tag="ONT65_001283"
     CDS             complement(17707..18108)
                     /locus_tag="ONT65_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328926.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18145..18654)
                     /locus_tag="ONT65_001284"
     CDS             complement(18145..18654)
                     /locus_tag="ONT65_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2328927.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18676..19533)
                     /locus_tag="ONT65_001285"
     CDS             complement(18676..19533)
                     /locus_tag="ONT65_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2328928.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19551..20384)
                     /locus_tag="ONT65_001286"
     CDS             complement(19551..20384)
                     /locus_tag="ONT65_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2328929.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20398..21195)
                     /locus_tag="ONT65_001287"
     CDS             complement(20398..21195)
                     /locus_tag="ONT65_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2328930.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21228..21512)
                     /locus_tag="ONT65_001288"
     CDS             complement(21228..21512)
                     /locus_tag="ONT65_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR2328931.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21509..22510)
                     /locus_tag="ONT65_001289"
     CDS             complement(21509..22510)
                     /locus_tag="ONT65_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR2328932.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22512..23579)
                     /gene="gnd"
                     /locus_tag="ONT65_001290"
     CDS             complement(22512..23579)
                     /gene="gnd"
                     /locus_tag="ONT65_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR2328933.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            23578..24426
                     /locus_tag="ONT65_001291"
     CDS             23578..24426
                     /locus_tag="ONT65_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2328934.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            25072..25278
                     /locus_tag="ONT65_001292"
     CDS             25072..25278
                     /locus_tag="ONT65_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR2328935.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(25480..26481)
                     /locus_tag="ONT65_001293"
     CDS             complement(25480..26481)
                     /locus_tag="ONT65_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2328936.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(26486..28399)
                     /locus_tag="ONT65_001294"
     CDS             complement(26486..28399)
                     /locus_tag="ONT65_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2328937.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(28567..29073)
                     /locus_tag="ONT65_001295"
     CDS             complement(28567..29073)
                     /locus_tag="ONT65_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2328938.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            29233..29673
                     /locus_tag="ONT65_001296"
     CDS             29233..29673
                     /locus_tag="ONT65_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2328939.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            29832..31172
                     /locus_tag="ONT65_001297"
     CDS             29832..31172
                     /locus_tag="ONT65_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2328940.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(31241..32398)
                     /locus_tag="ONT65_001298"
     CDS             complement(31241..32398)
                     /locus_tag="ONT65_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2328941.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT         9430 a         6459 c         5243 g        11299 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atgaattttt tcgtacaaaa aaaccgtctt ttctttcgaa
    13981 aagacggtta aaagtcaatt tttattaagc tttaacaatg tttgttgctt gagggccacg
    14041 ttgaccatct tcaacatcgt aagacactgc ttggccttct tctaaagttt tgaagccgtc
    14101 accttggata gctgaaaaat gtgcaaatac atcatttcca tcttcaccag tgataaagcc
    14161 aaaacctttg tctgcgttga accattttac tgtaccgtta ttcataaata aattcctcct
    14221 gatacgtatc aatgtacgtt ttttcgttgc aataaatatt tgataggcaa aaggagtttt
    14281 atctatgtag atatatcgct caaattacgt ttcaaaatag attactcatt taatatatca
    14341 tgctttttct aataacacaa ggataatgca atgaaatata aaattcattg tcttctattg
    14401 tcttcgaatc gaagaaaaag ttgaataacc gcgattgatt cactttttgt tttcttgttt
    14461 atttgctttt atctgcaact tttgacttta aaatttctta actatgaaaa cctttcaatt
    14521 ggtggtatac ttataaatgg aatttcgtta tcaacgcgtt cgttgccttt cagcaagtat
    14581 ttaaagaaag gagaatggct atgacattct ataacaaaga tatacaatat cgagtaacct
    14641 tggatacaga tttgaatcta tttgtcgttt ttgacaaaga aaataccaat catgtagcga
    14701 caggtgttac gatcgaacaa gcagtgcaag aattaaaaaa atctgcataa ggagtggaaa
    14761 ccctcatatg gacgattctc gttcttctct ttaaacgcat ctactggtag ataacagtag
    14821 atgcgttttg tgtttgttct aaattgttca ctacaaataa ggtagatact tcaaaataaa
    14881 aaattacttc tttttcatct gttccctcag tcgtgcagga caataaataa agaggactag
    14941 aaaatagtcc tctttattag agatacaacc ctttactttc taaaaatcgt tttgcttgat
    15001 gcaggtcata aaattcatag tctgctttat tggattcata taaacttaca tctactttca
    15061 acttatgctt cccattatga tcgggcaacc ctatcaaatg atctttgatg agaaaagaca
    15121 aaatgcccgc tcgccttgct gcttcgatcc ctgcaatcga gtcttcaaat acaagtgctt
    15181 cacttggagg aatattcatt tttgaacaag ctttcaaaaa gatctctggt tccggctttc
    15241 ctttttttac atcatcccct gaaatgattt gatcaaacaa ggcatataga ttttccattt
    15301 ttaaatacat ttctactttt acccgtgtat tagacgaagc cacgattgta tggattcctc
    15361 tttcttttgc aaaagataaa atttcttctg ctcctttttt cgtatatact ctcttatctt
    15421 ctttcacgat tttttcttta tattgattga ttgcttctcg ccatttgata tatggaacat
    15481 cttctccgta caaaatgccc atttgttctc ttatcgccat ttctgtttgg ccagttagta
    15541 aataatagac ttccttacgc atttcataat catacttttg tgctgatttt acataagcac
    15601 gataagctaa acgtccagtt tctaataaaa ggccatccat atcaaagatg aagagcttga
    15661 ttttattcat aatgctctcc cagtggtttt tccttactta tcgcctcata tagaccttgc
    15721 atgtaagtga gtcccattgc acgatcatac aggccatatc caggcatagc cacttcaccc
    15781 cacacggttc taccatgatc agggcgtata ggaccgtcga acccttcttg caccaacgct
    15841 ttcataatat gatacatatc aagggagcct tctgtagagg gatgtgccgt ttctttgaac
    15901 tttctttctc ctaaaaactc cacgtttctg aagtggacaa aatggatgcg cgaacctacc
    15961 tcatggatga tttcaactaa atcgttttcc ggatttgccc ctaaagatcc tgtgcacagc
    16021 gttagtccat tatatggaga atctactgca tttagaattt tcttcaagtc gccaagattt
    16081 ttcgtcactc taggaaaacc aaaaagttcc catggcggat catctggatg gatagccatt
    16141 tttacatctg ctttttcgca cactggaatc actttttcta aaaaataagt cagattttca
    16201 aataattctt cttcggtcat tccttcatac agtttttcca gctgagcgaa ttttttcaac
    16261 cgttcttctt cccaacccgg caatttgaat ccctttgact ggctgtgtat caattgatac
    16321 atttctcctg gctccatctg atcgacgatt ttctgatcgt ataaaagaga atagcttcca
    16381 tcgttgtttt cattaaacaa atcagttttc gcccatccaa aaattggttt gaagctataa
    16441 caaatcaatc gaaccccaca tcgagacaag tttttgattg ttcgtatata gttatcaata
    16501 tagtaatctc gttcatctgt ccctgccttg attgcatcgt ggatcgctac actttcaatt
    16561 ccaagcagtt ccagtccttg gctctcaaca gatgctttca actcctcaat ttctgaaatc
    16621 tcccaaacat ctccaggcat tttagtcagc aaagttccaa ttatcccatt tattcccggt
    16681 atttggcgaa catattttaa tgggatcgaa tccccttttt caccatacca tctaaatccc
    16741 cacttcatct tttcagcctt ccttctaaaa atcgtcctca tctaattttt tttcttccgc
    16801 tgaagcatgc caaacagtta aattatcttt tccttgtttg acaatttgtt ccaccgcttc
    16861 ttctatcggt gtgtgaagca tctgatgatt gattgcttct aacaacattg gcagattcac
    16921 gccccctatg acataagtgg ctgctttctt ttcttcagtc agttcattga ctgccaagag
    16981 cgcttgattg tacggacttg cgctaattag atcaacaaat actagcatgg gttctgctgt
    17041 gttgatcata gttaattttt ctttgacctt ctctccgaat gtttggatgt cctccccaga
    17101 ctctaagcac attgtttcaa tatggctcga tgtcccgaca attacttccg ccgcatcttt
    17161 cagtccttcg ctcaacttac catgtgtaac gatcatgatt tccaacatta gtcttctcac
    17221 tccttgtttt gcacttttta cttatcgaaa aagagcaata atctgattgg cattttgcca
    17281 attaaatgtt ttccctcgta aatctgtttt ggtcaaatag aagaactgcg cttgcttttt
    17341 tcttgcacta aaaatataat tgacgttttt ttctttcaac ttatcaactt gtttcagcca
    17401 ctgttcttta tttgaaaaat aatttcccga aaactgtagc ggtgtatctg ccccaacaga
    17461 gtctagataa aaaagaatag cctctttttt catacccgat ctcacgctgt ctaagccctt
    17521 ttctccataa cagccttcat caacaaacgc atacgctaca ctcggatcat caatcgtacg
    17581 gatcatttct agcatcgtta atatggatga agtatttcga ataaaagttt gtctgctagg
    17641 ccatccgcga gctgcttttc ccaataagaa aaagtagctc ccaaatgcta gtacagtgat
    17701 acctgtttat acggatagca gatcgaatac gtgatgagga tttttgttat atatcatcat
    17761 tccccaccaa cctaaactga acaacaaaat gaaggacaat aatatagagt agattgtttt
    17821 tttcctttgg tctttccaat caaacatgaa ataacttcct aatgcatgaa cgggtgtatc
    17881 gtaataagta cagataactt tctctgcttt ttcgatatct ccaacataaa tattttttga
    17941 tcggtaggct agcgtatcgt aagcaatgac tgtcacatct gttctcatac tgtaaatatc
    18001 tgatactaat gctgaaagaa aacgctgttt actctttttt gtacggcgct tacggtaaat
    18061 atagcgataa cgaaattccc aatcacgaaa cagctcgttc ttcttcattt tttcgccctc
    18121 tttatctttt ttctaagcat ttgtttatga caaatcaaac ttgttcaata catcttttaa
    18181 tcctgtttta ggtgcagatg gagtggcttg aaaataaata ttctttacgc catattgttc
    18241 attcatttct ttcaattttt cagcatctga tttatttaaa taaacagatt tcgtcactaa
    18301 aagatctgta tcaggatttt tttgagcaat ccctccaata ttcaattctt ccatggggat
    18361 tccgtgtttt actaagtcgt aaatcgtttg gactgttttt gcgatcaaaa tacaattgta
    18421 ttctttaaat tgatattcat cccaatagaa tttagctttc tctggtgtaa tgacaattgt
    18481 tttttgtcct gtacggcttc ctgcattttt gtaaagatct ctcataaatt tatcttttgc
    18541 tacaacttcg tctacaacaa aaatggaatt cactccactt ttttgagata acgttaccat
    18601 tacttgccca tggatcaaac gctcatccac acgcgctaaa ttgactacac ccatcttatt
    18661 cacgttccct tctttttata aaataccgat tcctgctagc acaattaata ttccagtcag
    18721 ccataataat gcttgtgtga ctttcaatcc ttttttcgta tagaacaagt acacacccat
    18781 tacgacagct aaaggtaata tccctggcac gatttgatca agaatttcct ggataacaaa
    18841 ctcacgtccg gatatcgcaa atttcaatga agattctact tttacatagt ttcccgcaag
    18901 cacccccatc ataaacaacc ctaaaactga tagcatttca atcgctgtct gaacaccagt
    18961 gttttgcttt aggcctgtag cattacggcc catagtgaaa gcttgtcttg cgaaaaatac
    19021 ccctagtaaa acctgataca acccaaagca gataaaagga aaaagtgcgc ctaaaggact
    19081 tccctgttgt gcccaaggta ccgcgattgc gataaaaata tattggacgg ttcctgaatc
    19141 gattgcatct ccgattcctg ctagtgcccc catcaatccc gctttcgtgt tatacatcaa
    19201 gtcatcctgc atcaagatct ctttttcttc atactcttct tctgctcttt gctgttccat
    19261 agaagacatt aggccagtaa tcacaccgcc accccagctt aattgagtgt tgaagaacag
    19321 taactgccgt ttatatgctg cttttaaaga gtcttcatct ttataaagtt ttttcaagat
    19381 tggcatcatt ccgtatagta gtgatggcgc caagtaacgt tcaaaagagt gaggaatttc
    19441 gtttgcaaaa tgccatctca gataggcttt tgttacatct tttttcgtga tttcttcagg
    19501 agcacctgtt tgattatcca ttatttcagc catttcccat tcctcctaaa ttaaaatccg
    19561 tcatcatagt catcttcgtc atcaaaagat gctgaattgt tattgcttgt ggcaggctgt
    19621 acttgtttcc ctttttgcga ctggacaaag atcaaagcga taataatccc aaaaatggca
    19681 taagtaacca tcgtaatatc taaagatttc aaaacaacac tcataaaata agcaaggaag
    19741 aaaaagacca gataatcttt tcgaccgata acgtatacag ttgtcgcgat accgattgcc
    19801 gctaaccctc cacctactac ttcaagaatg tggataacga ctgttccctc taaagctttg
    19861 attacatcag cgatcaatgg cgcccctaaa tataaagcaa tgaaaacaag cggaacgaat
    19921 aataaaaatg aggcaaccgt tggataaaga ataattgatc gggccatagc ttttgtattc
    19981 aaatcttcta cagctttgtc tgtatattta cctaaaaaag tattgatgaa aaaacgaaac
    20041 tgatacaagt aacttccgag caatccgact ggcaccgcaa cggcaatcgc tgcttctggc
    20101 gacagatccc ctaataatgc gactggtact gcgatagctg ctgcaataga tggttcagct
    20161 ggcattgatc ctcctggaga aaaaaccccc atataaatca tttgcaaagc ggctgtaaca
    20221 atcatcgctt gtgcaacttc ccccatgatc aacccgacga ctaatccagt catcaaagga
    20281 gaaaaacgta aggtcaacgt ggctcctcca cctaaccagc gggacataac cgcggctacc
    20341 cataaagcaa tcaacaaact ttgtactaca cttaatgttt ccatgtgtcc tcctccttca
    20401 gatctttctg atataacctt ccagttccat cattgactgt atcagtgatt tctgtgttac
    20461 ctctatagga atcaaatgca ctttctcttt cgaatcaata aattcagcca ttttatctat
    20521 tatctgttta tctttcggaa ataatcccat ttgttctaga gaaaccggca gttctaattc
    20581 ccgatacaaa ggatataact cgtctatttt ttcccagcga ccttctagtg ccagttgata
    20641 gaaaatccca tacgctactt tttctccatg tagaaaacga tggctttccg gaatatattt
    20701 actgatagcg tcatgcatag catgtgctgc cacatttctt gcgtatttat ctcctaatcc
    20761 tccaactaaa ccagcaacag caaagataat ctctgaaagc tgtttgaact ccgatgtcag
    20821 ttttctttgt gccatatctt ggattgcttt agcagaatga ttcaaaatct cttctttgca
    20881 gatcttcgca gtatcttgtg ctagcttcaa aaatgattca tcttgcaaaa aatcttgttc
    20941 taagatgagc tcagattcat accattttgc tagtgtatct gctaacccgg caatgaaata
    21001 tggaacaggg gaatcaatca ctaattgggg atctgttata aaaaaagcag cttgtctgta
    21061 aaagtgttct gttttccctt ctgcttgtcc attctcctta tacatgacag caagaggtgt
    21121 ccatggagca caattactag ctaaagtagg gatgactccg aaatcgattc ttgcaagatt
    21181 ggcactataa cccactagat cggtcagttt tcctccgcca acgccaacta cgaaatcgat
    21241 ttcatttttt tgatgattgt agctattcgt ctggcttcag cataactaca ttctccagaa
    21301 taacgatgat aaaaaaattg aatcgtttcg tcattcaaaa atactaattt aggtagcgcc
    21361 ttttcccatg ataccgttcc gtgggtgacc agtactcttt tcgcgccgta tgatttcaag
    21421 agatcagaaa cttttgaaat accaccttct tcatataaat aaaattgagg gcctgttttc
    21481 acttttaaac cgttcaccat tttcttcatc atccttttat atagttgctc tttgtctgga
    21541 cagaacgttc aggaagaagg tgctgactga tctgttcgac atctctgaca catttttcac
    21601 ccattgcttc taaacattcc cttgtgtagg ccgatatatg tggcgtaatc actacttgtt
    21661 caaacgctaa atacggatga tcttgacgtc ccggttcttc ttctaaaaca tctgtggcat
    21721 atcctgcgat ctttccgctt ttcagaccat taatcatcgc ttcttcatca actagagcgc
    21781 ctcttgcact attagataga taaacatttt ctttcatctg atcgacttct ttatcagaaa
    21841 tcatgtgata gttctcttct gtcaaattcg cacataaaca aatgatatct gattgttcca
    21901 gtaattcttg aagttctact ttttttgcac caaagctttc gatgtataaa tctgatttgt
    21961 aaggatcata agctagaaca tcacagcgaa aaccgttacg caaaatttcg attactccgc
    22021 ttccggtatt tcctgcacca atcacaccaa ctgttttgtt aaacagcgtc ctaccgacga
    22081 actccgcccg atcttcccat cgatcgtcct tgactcttgc ttgtgcttgt gtcgtgcgtc
    22141 gaagcatgga caacaaatta gttatattat tttctgctac agcatttcgt tcaactaaag
    22201 caggtatgat agatacgatt gtctggtgtt cttttgctgc ttttaaatca atattgttgt
    22261 aaccaatacc atgtcgggaa atcaatagca gttcatcttt gttttcaaaa aaatctcgtc
    22321 gaaaaaatgg cgtaacactt gcaataatga tattgaatcc tttaagtaac tccgctagtt
    22381 cttttcctgg aatctcttga tcaacagtaa agaaatgaac gtctccaact gactttaatc
    22441 tttccaagtg gtgtggaaag actcttccga aactgctgga attaacgatt gctatatgat
    22501 aatctggcat tttactcacc cttttcaact atttcatgtc cgccaaaccc atttcttagt
    22561 gctgaaacta cttttgctga aaaactatct tcttcctgtg aaagattcct catgaacaaa
    22621 ctagttgcaa ttactggaac tggaacagcc atttctagcg ctgtttcaac cgtccatttt
    22681 gcttcacctg atgcagaaac gattccccga taattactta aattgggatg ttcgtttaac
    22741 tgatcttcca tgatttccat cagccagccg cgaatcaccg aaccatgatt ccaaacacgt
    22801 gcgacttgtt ccaagtcaaa agcatactca cttttttgta caagttggaa tccttcaccg
    22861 attgcttgca tcattccata ttcgattcca ttatggatca ttttcaagaa gtgtccgctt
    22921 ccggatgcac cagcatacag atatcctctt tcaattgcta catctttgaa taattgttcc
    22981 acggatagaa aagtttcttt atctcctcca atcattaagc aggcttgatg tcgtgctcct
    23041 tcgattcctc cagaagtccc acaatccaag taaccgattt catttttctg aaactcttct
    23101 gctcgacgga ttgaatcttt ataataagag tttccgcctt caataataat gtctcctggt
    23161 ccgagttttg gaaaaagttt tcttaacatg ttttcggtta tctctccagc aggaagcatt
    23221 aaccagatga tttttttctg ctcaaaagta gctagaaatt cagagatctc ttgataagca
    23281 ggaatgtcat tttctcttgc ctgactaaca gctgtacttg aaatgtcgag tccttgtact
    23341 ttgtgatggt gctcttttaa gtttaaagct aaattcaatc ccatttttcc caaaccaatt
    23401 acaccaattt ccatgatagc gcctccattt cttataccct cattatacaa aaaaattttt
    23461 ttgtaaatag attttacaaa aatatttttt gtttgatttt ttcctactct ccttgtatct
    23521 ttaacattaa tgagaacgga aaaaatgata atcttattat gaaaatgagg aattctcatg
    23581 agcgtctcca taagactaca aatcaaaaca atgtacaacg acttcagccc aaaagaacag
    23641 gctatcgccg attatatttt agaaaatcca agcaaagtaa gtcatagtcc tattagcaat
    23701 ttagcaagtg aactgggcat tgctgactct actttttttc aatttacaaa aaaacttggt
    23761 tataatggat ttaaagattt taaaatggcc atgttgatcc aagaaaatga tctatctgct
    23821 atttctatcc acgaaaatat tcagaaaaca gatactgagc tcactatggc gcaaaaggtg
    23881 ttcgagtcta gtatttcaac tttaaatgac acaaaaaagt tgttacaaca aaaggatcta
    23941 aaaaaagcgg ctactattat caatgaatct aaacgtgtct attttttcgg tgttggcggt
    24001 tcagagattg tagcaactga cgcttatcat aaatttttgc gttcccctat cgcaacttct
    24061 cacagcacgg attaccatat tcaattgatg gaagcttctt tattgacgga aaaagactgt
    24121 gctgttttga tttctcacac agggcaatca aaagaaacaa tccatattgc tgaaaccgta
    24181 agaaaaactg gagcaaaaac aatcgttatt actagccaag ctaattcttc ccttgctaaa
    24241 cttggcgatg tcgtttttat ttctatttct gaagaaacag aattccgatc tgaagcttta
    24301 gcttcccgta tttctcaact aagcattttg gactctctat atgtgatttt gatgttttac
    24361 aacgaaaaag aatcacgtcg ttctatcgct aaagttcgac atactatttc tgaaatcaaa
    24421 gaatagcacc ataacagaaa aagcgaaaaa tccataaaac aaaaaaagcc actaccaaat
    24481 atttggtagt ggcttttttg gttctgcaag ggtggtacag aacatcaata gtcggtgggt
    24541 tagaacgtat gtcatcgcat ctttggagaa ggttgtattg gtattattcg agttacttgt
    24601 tagatgcgat aacaaagtta gtatacagta gtaaaaaatt tatcttttct tttttttatt
    24661 atttttgttt cttattttct aaaaaattaa aaaaaatttt ggtgaattaa gtatttcctt
    24721 gataacactg gcttttggcc tcattttagg ttatcatttg taattttatt tcacagttga
    24781 tatgattgtt atgtaaaata aaaaagttta ggatacatcg aatttttggg gaaattcttt
    24841 tgggggtatc ctaaatcttt tctattatat tcccatattt tctattattg gtctagacgt
    24901 atgatggtaa actttgtacc tatctgattg ctgaatcatt aattgcttat cattagccat
    24961 ctttgtttca agttcgctag atgatcattt tattcacatc ctaacaaact tgtcacattt
    25021 atagattctt aatctattta tcatacaatt ataaaaaagg aggtgctgct tatgaaagct
    25081 atccatattt ctttgctttt tttcgctagt gcattgattt tatttgctat tcatctttgt
    25141 tcccagcctt atatcaataa caacggaata ttagtggaac cagcattctt ttgcttacca
    25201 ctcggctttt tgtcccttct tgcctccgca ggcttcggat ttgttgcttt ttttaaacga
    25261 tcaaaacaac aaatctgact tataaaattt acgtaaaaga gatcgtgaca ataatcgtgt
    25321 gatattccca aatttcgaat aaaaggtgcc ccttgccatc tctaactcct cagtcacaag
    25381 ttacaatatt cataaaccat gaaaagcttg tttattgaaa ttgctccttg tgacttggag
    25441 ctaagtgaca gattacaacc tcttttgcat cataagactt tactccttga aaaacattac
    25501 taacttttga tatgtttctt gcgcttgttt attgctgaaa tcaaactgat attcatggtt
    25561 caaaacagca tttttctccg cataatctac ttttgtgact ttcacccccg ctttctcaaa
    25621 tacagaagtt acttcttcca tttgtttcgt aaacgtattt gtatttccat ccgttaagaa
    25681 tgtgttttgc gtccaacgat ccgcatgtct gaccaaggct aaacttttga ccttctctga
    25741 ttgttcaaag tccttattat caaagtaagc ccatccacat ttttcataaa gaaagctaga
    25801 gattttatca cttacttgag taagtgcaga aggatccaac agaccagaca aagaaataaa
    25861 cttcgtaatc tgcttgttct ccaaagtcgg atcaagatga tcagcttttt taatttcttc
    25921 attttgaagt gctaacaaat attctccact aatttgtgca cctgccgaat ttccaccaat
    25981 cgaaatattc gaccaatctg cttgatcttt tatctctttt ttagctgctt gaatggcttc
    26041 gttcaattgg ataactggtg taggatgttt tgcttccggt gatcgttcat aatctatatt
    26101 tacgatgatt tgctgcgtgt ctgaacatag atattccaaa taatcacgac aatcattttt
    26161 atcccctcct acatatgcgc ctccatgcat ccagaaaaga atcggtaatt ttttcttttc
    26221 cactttcggt gtgtagatat ccatattatt atttttgtac tttgaaggat aggatatttc
    26281 tttttttata tcgatttgtt cgattttttg ctggaaatca tctggtcggg catatacttg
    26341 ttctttttca cttgtgtcaa attgttttct aacaagcaag ctaatcggct ttggtgtgat
    26401 ttgattaatc aaaaatacag tgactaaaat aactacgatg ataactggta ctgcaatcac
    26461 tattcgcttg aaccatttca ttcatttacc ctctctttct tcgccaaatc catgtgataa
    26521 tcgtgctaac agataaaaat ccacctgaat aaatgagaag cgaatgtttc ttttctccag
    26581 ttttcggcaa cattcttttt tttgcatctg tctttttttg tactttcact gtcatctgaa
    26641 tcggttgcgt atcatcactt gattgatcct ctcgaggttc attctttccc ttattgtctg
    26701 cattctctgt cggatcttgc ttttttggtg gagctactgg gatttttttg taatagaaaa
    26761 taatagttgg tatcgtctca ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt
    26821 cgtatccttc gaatgtcttt tgctctgtca tataattttc tccataagct ccgtaaagct
    26881 ggatttcgtc ctggtctttg agtagacttt tattgttttc atccacatac tttacgttta
    26941 tctgatcatg gaacactact ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt
    27001 caacttcctg tgcctttcca tgccctgctc cttctactaa taaaagatct ttataccctc
    27061 ctgataactc atataatttt tgcgcgttag acacaggtac tacatcatct gctgttccat
    27121 ggataatcaa tttaggcaat gaatctgaca ttccaatcgt aggtagagca gcttttgtat
    27181 ccaaatgcat ctcctgatca aaatagtatt gtttcaaaag gcccataaat aaatcttcat
    27241 gctcttgatc cccaatgatg tctaactgat tttttatggc atccggtagt gcgggggcta
    27301 ctgtctggct gtacgtaaaa cgtagttgtt catcaattga tgcaaatccg caattttcaa
    27361 taattccttt gacattgaca ggaatatctt tcgctaatac agacatcatc gtagctgctc
    27421 ccatcgaacc accataaagt aaaatctttt gagatggccg ctctcgaact tcttgattta
    27481 tccagtaaag gacgtcatct gactcatatt gtccataggt cacataatcg cctccgctat
    27541 taccagtcgc tctggaatca acaaataata aattataccc ggccttataa aaatcattgt
    27601 attcttctgt aacgatcccg ttattccaat tccctcgaaa tccgccatgg atcatgactg
    27661 ttttatctga accttgatcg acataatatg catatacatc catcattttg ccatcttgtt
    27721 tgtttagtac agtttgatga agttgttttg tatttggata gaagcctgtg ttttctgtag
    27781 gcgttgtccc atcatctgta cctccaagca tcgacatcaa cacactgttt tttgcaaaag
    27841 cgatatcgta caatagataa gtagcaatca gatgtttgat aatctcttta tctacaccat
    27901 aatcattaac agcaagctca gtgactaata actctattaa ttttgacttg atgtcttctg
    27961 ttggtccatt aagtatttct gtcatctgtt taccgaagta attcaaatat tctttaccat
    28021 tgaaattttc acctttaccg atatcatcat tttctgtccc attctctttg tcacttactt
    28081 tagcagaaga ggattcgtcg tttgacattt ttgtagctgt tgcttcttct gtttgttggt
    28141 tatctgtggt agcggtcgtt tcttgatctg tgctagtaac agatgtctca accgttgttt
    28201 cagtatttgt ttctgtttgt gaagtactcg atttcgtttc atttccagca tcagtagttt
    28261 ctgtggtttc tacctcccga gattccgatt cggaagaaat gctctctcca tatactgtcc
    28321 attgcgggac gcttgtactt acagcaaata agatgaccaa cgctcccttt aatacttgtt
    28381 ttgttctcaa gctattcact cctttttatg tttctaggag aattatatgt aaacgcaaac
    28441 attttttata caaaaaataa atggtttatc ccataaaaat agaacaggat tattttctca
    28501 ttttacgtaa taaagcagaa tggataaacc agtatccatt ctgctttttt taaacgaaga
    28561 aacaacttat tgctccaaaa atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc
    28621 gattgtttcc tttgctttca taaaaggatt atcataagtt gtcgttgctc ctctacacat
    28681 cataagctgg tatcctaatc catgagcaaa cttcacagtc gtatctatac aatactctgc
    28741 ttgtgcccca cataattcga tcgaatgaca gtccaattca gttaataaaa cactcaaatt
    28801 agtcttataa aatgaattcg catgggtttt tctcacaaaa taactgtgct caggaataga
    28861 taattccgac aatagctccc atggttttgt ttgtggcact aattgagcat cttcgtgttg
    28921 aacgaaaata actggtttcc caatcgtttc gtatgttttg attcttttat tcactaatgc
    28981 tgtcactgtt tctaattcgt gtatcggcga agaatcaaaa catacccctt tttgtaaatc
    29041 gatcactact attgcgtcgg ctgtgtttga catacttgtg ccttctttct tttttcttga
    29101 tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt cttattttac
    29161 acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa tacttagcta
    29221 ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc ttcaagacgc
    29281 gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt aattcgtcaa
    29341 ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg cgtgaccgaa
    29401 atgctctcta ctcattctat tagttataaa aatcttgttc acagaataga tgaactcagt
    29461 ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa accgattatc
    29521 tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag gatgttcctt
    29581 tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca tcttgtcgat
    29641 tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc tgcttttctg
    29701 gatctataat atctagtgtt ggtgacgcct tctgttgcca atactttttc ttttttctaa
    29761 taaacgcaaa cagacacctt gaaatccttt agaataaagt cgacgaaaac cattcaaagg
    29821 agtgtttcaa gatgtcctat tcagagttta ccaaagagtt actagatatt ctagacctaa
    29881 atcttacctt tcatgaagat gcctttcgca aagagcgcat taatgacgaa acatgttttg
    29941 tttttgatgg cacattgacc tatcaaccag aggaatgctt ccattgccac tatcaaaata
    30001 agcaaacgat cattaaatgg ggctggaaaa aagtatccat cttattgaat gatgtcagta
    30061 actacaagac cattgtgcgg attaacaaac agcgcttcaa atgtaaacac tgtggaaaga
    30121 cttttttagc agaagattca gttagcgatc gtcgctgctc cattgcccgc agagtcaaac
    30181 aagccattct tgagctatta agtgagccta tttctatgtc tttgattgca cgtatgaagc
    30241 acatttctcc gaccactgtc attcgaatac ttcgcagcct tcggcctaaa accgtttctt
    30301 tgaaccagcc gttaccagaa gtggtgtgtt tcgatgaatt caaatcggtg aaaaacgtct
    30361 cgggagccat gagctttgtc atgatggacg gcaagaccca tcaactgatt gatattgtgg
    30421 aaaatcggca actcaatcct ttacgcgact atttcttgcg ctaccctaga aaagtccgtg
    30481 aacaagtacg tctagtggtt tcagatttct atacacccta tcgcacattg gtgaaagagt
    30541 gttttccgaa tgcccgaatc gtcgccgatc gctttcatat tagccaacac atcggtcggg
    30601 cctttaccaa ccaccgaatt caagtaatga agacctttaa aaaaggggat cgtcggtcga
    30661 agcacttaaa gaaatactgg agactacttc aaaagaatgc gtgggaattg aatggtcagc
    30721 atcgctactg gcgtccgtca tttcgagatc atctgacaga agcggagata gtcgatcggc
    30781 tattatatta tgatgactcc ttaaagcgag gctacgaagt ctaccaggtt tttctttccg
    30841 ctatcagaag acaagatgtt cctgaattcg tcgcgctcct aaaagaagac tacaaggagt
    30901 tgccagagca ctatcaacca gtgttcacga ccttcaagaa atatcgaaca gaaatcaaac
    30961 gagcgttgcg agttccctat tccaacggcc ctatagaatg tttgaacaac catatcaaag
    31021 tactaaaacg aattgcctat ggctttagaa actttcaaaa ctaccgagaa cgaatctttt
    31081 tatatcgagg aaaatatttc aagaaaacta agaacactac ccaactgact aaagccagaa
    31141 caacaacacg gctagacaag atagctgtct agccgtgtga gtatctcgat tattctattg
    31201 aatttcttca ccaacactca ttgacgaaga gccgcttttc ttaattaacg aaatccaatt
    31261 tttccaatat ggatctattt ttctcattcc tgatttttat gatttcagct ttcagacgcg
    31321 cccaatcatt ttttgtaggt tgtccattcc agtaaaatat ctgtgtattc gtttgatcat
    31381 atgccttttt gtcgatagga taatcgctaa tgacaatatc aggtaactga tcagcggatg
    31441 cactatattt tattgggcgt tcagtcatag ataatatcct tttctttgcc cataattcct
    31501 gtgttttacc atactttgat tgaatataga atatgacagg ttgttcgtgt tttataaaaa
    31561 tatccctgac taacatacag tattgaaaaa tcaaccgtct gttcttttca taataagtat
    31621 taagctctgg aattctgcga gctaccccac taaaaaactg ttgcatgatt ggatacatca
    31681 ctggaaatat atgctgaaaa tctctgtctt ctgctgattt tccaaaagca tccaccttct
    31741 tttttgtccc aaaaaccctt gaatacaaat ggatcattgc caaattgaca tatagataat
    31801 ttttttctga agttgtaaaa caaatcttga agtttttttc aaaattttct acaatagacg
    31861 attcaatcca gccccaactt tcttgtactt tatctggagt atccaacaaa tcttcttcca
    31921 aataggtgtt cattacacca aacataaaat aaaggtaagc ttgctctgtt ggagtgatgt
    31981 gattcatcga ccagaagttt gcttcaaaaa aaggctctag tatgtaatgc gtaaactctt
    32041 cttttgttat caatggatgc acaaatttgt tcatctcttc tgggacatac gtaatcgagt
    32101 ggccttgttt cattcgttgc aacgaaattc ttagtaggct gatccatttc tttctatcta
    32161 tactacgcgt atatggcgca tgtttcgcaa atttttctaa tagttgacgt ttatttttta
    32221 tacttgataa tatatcagaa tgattttcgt cgaatgactg ccaatacatc aagtgaaaaa
    32281 agtatcggat acggtattct tctccttcta tcggattacc ttttttcaag ctgcaagata
    32341 aatgatagtt tttcaaaata gtattaagtt ctcttaattt tctttgaatc gtacgcaaac
    32401 ttatataatt ttttgactaa gattttctaa g
//
LOCUS       DAJSBC010000026        32178 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSBC010000026 DAJSBC010000000
VERSION     DAJSBC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.026"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(409..768)
                     /locus_tag="ONT65_001299"
     CDS             complement(409..768)
                     /locus_tag="ONT65_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR2328942.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(798..1139)
                     /locus_tag="ONT65_001300"
     CDS             complement(798..1139)
                     /locus_tag="ONT65_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328943.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1136..1810)
                     /locus_tag="ONT65_001301"
     CDS             complement(1136..1810)
                     /locus_tag="ONT65_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2328944.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2986..4284)
                     /gene="asnS"
                     /locus_tag="ONT65_001302"
     CDS             complement(2986..4284)
                     /gene="asnS"
                     /locus_tag="ONT65_001302"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR2328945.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4324..5514)
                     /locus_tag="ONT65_001303"
     CDS             complement(4324..5514)
                     /locus_tag="ONT65_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2328946.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5535..6062)
                     /locus_tag="ONT65_001304"
     CDS             complement(5535..6062)
                     /locus_tag="ONT65_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR2328947.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6109..8898)
                     /locus_tag="ONT65_001305"
     CDS             complement(6109..8898)
                     /locus_tag="ONT65_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2328948.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9045..9245)
                     /locus_tag="ONT65_001306"
     CDS             complement(9045..9245)
                     /locus_tag="ONT65_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2328949.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9698..10018)
                     /locus_tag="ONT65_001307"
     CDS             complement(9698..10018)
                     /locus_tag="ONT65_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328950.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10521..11639)
                     /locus_tag="ONT65_001308"
     CDS             complement(10521..11639)
                     /locus_tag="ONT65_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328951.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12110..13417
                     /locus_tag="ONT65_001309"
     CDS             12110..13417
                     /locus_tag="ONT65_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR2328952.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13538..14737
                     /locus_tag="ONT65_001310"
     CDS             13538..14737
                     /locus_tag="ONT65_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2328953.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14764..16032)
                     /locus_tag="ONT65_001311"
     CDS             complement(14764..16032)
                     /locus_tag="ONT65_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR2328954.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16202..16843)
                     /locus_tag="ONT65_001312"
     CDS             complement(16202..16843)
                     /locus_tag="ONT65_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR2328955.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17252..17755)
                     /locus_tag="ONT65_001313"
     CDS             complement(17252..17755)
                     /locus_tag="ONT65_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2328956.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17878..18642)
                     /locus_tag="ONT65_001314"
     CDS             complement(17878..18642)
                     /locus_tag="ONT65_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR2328957.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18750..19025
                     /locus_tag="ONT65_001315"
     CDS             18750..19025
                     /locus_tag="ONT65_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR2328958.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19585..20535
                     /gene="yidC"
                     /locus_tag="ONT65_001316"
     CDS             19585..20535
                     /gene="yidC"
                     /locus_tag="ONT65_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2328959.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20581..20982)
                     /locus_tag="ONT65_001317"
     CDS             complement(20581..20982)
                     /locus_tag="ONT65_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2328960.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20996..21805)
                     /locus_tag="ONT65_001318"
     CDS             complement(20996..21805)
                     /locus_tag="ONT65_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2328961.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21792..22682)
                     /locus_tag="ONT65_001319"
     CDS             complement(21792..22682)
                     /locus_tag="ONT65_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2328962.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22695..23174)
                     /locus_tag="ONT65_001320"
     CDS             complement(22695..23174)
                     /locus_tag="ONT65_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2328963.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23350..24291)
                     /locus_tag="ONT65_001321"
     CDS             complement(23350..24291)
                     /locus_tag="ONT65_001321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HCR2328964.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24288..25283)
                     /gene="lacD"
                     /locus_tag="ONT65_001322"
     CDS             complement(24288..25283)
                     /gene="lacD"
                     /locus_tag="ONT65_001322"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2328965.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25276..26472)
                     /locus_tag="ONT65_001323"
     CDS             complement(25276..26472)
                     /locus_tag="ONT65_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2328966.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26490..27218)
                     /locus_tag="ONT65_001324"
     CDS             complement(26490..27218)
                     /locus_tag="ONT65_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2328967.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27659..28633)
                     /gene="lacD"
                     /locus_tag="ONT65_001325"
     CDS             complement(27659..28633)
                     /gene="lacD"
                     /locus_tag="ONT65_001325"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2328968.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28630..29088)
                     /locus_tag="ONT65_001326"
     CDS             complement(28630..29088)
                     /locus_tag="ONT65_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2328969.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29085..30548)
                     /locus_tag="ONT65_001327"
     CDS             complement(29085..30548)
                     /locus_tag="ONT65_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2328970.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30530..31462)
                     /gene="pfkB"
                     /locus_tag="ONT65_001328"
     CDS             complement(30530..31462)
                     /gene="pfkB"
                     /locus_tag="ONT65_001328"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2328971.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31604..>32178
                     /locus_tag="ONT65_001329"
     CDS             31604..>32178
                     /locus_tag="ONT65_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2328972.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFF"
BASE COUNT         9385 a         6920 c         5511 g        10362 t
ORIGIN      
        1 atttcttcca tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgcccctg
       61 ctcttatcca acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc
      121 ctgttgctgg caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa
      181 tccctgcaaa atgtccttcc cagatattcg aagctccact acgctttcaa aaatatagaa
      241 agagttcttt aatgaaaatt atttcaactt tccataaaca gtaatcccat ataaataaaa
      301 gacgtaaaag tcacatggga gaacttatac aggaaaacac atagagtcag gtgaaataca
      361 tgtatcccac ctgactctat gcgttttcta acctttatta ttctcttttt atacaatttc
      421 tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat
      481 ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag
      541 gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact ggtgataaat
      601 tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat
      661 tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc
      721 gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct
      781 tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct ttttttagct
      841 tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc ttttgttact
      901 aatgtgtgtt ggattagttt tcctgatggt cgctgatcca cccgttcttc tgactgttgc
      961 acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt
     1021 ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca
     1081 gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccgtat ttccatcatt
     1141 tctcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg gcaattgcat
     1201 ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc
     1261 ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatgcca tctaaaggaa
     1321 ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt
     1381 ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga
     1441 tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt
     1501 gctgcgcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat
     1561 tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc
     1621 gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaatttca
     1681 tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat
     1741 cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct gtcaccgata aataatatac
     1801 gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta
     1861 tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta
     1921 gtcccttcaa tcaattgaat gaacgctcat caagtgtcta gttttttata ctttttttct
     1981 taacgtgccg gattcccctt gattccttcg ttgtttggtc cagagaaatc gatcacacct
     2041 aagcttccgt caatcagacc actgatgacg ccatcagaac atatatttat aggctcatct
     2101 aagacttcca gtaaatgttc ttttactaat atctcttcta gattgcttgg gatagattgg
     2161 tcataacttt atctggtccg atgatttcaa ggatgaatta aacttacata tggatcattg
     2221 catgctgaaa atacggacct actccatctt cgaatgttac tattagtttg ttttcaggta
     2281 ctagatgctg ctttatttca acagcagaag catcatcaaa tcagatttcc atttgaataa
     2341 ccttctttgt atatgtttga ttgattgcga tttcgttata gaaccttgtg aatcaaatag
     2401 caatttcttt tgctttcttc tcttgtctgc tggaaacttc ctttatcccg tattttaaaa
     2461 aaagaactgc gagagttcgc agttcttttt tttaaataaa cgttgctctc cggccaactc
     2521 ctcggtcaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt
     2581 gtgactcgga gctgaacggc aggcgcacaa cctcttgata taaacggtgt tcaatcagct
     2641 gaccctcgat attagctaaa aaaacggtaa aatatgagaa gctatttttc cgttttgttt
     2701 ctctaatact caggtcaagg cactgatttc acaacctctt ccttaaacgg tgttcaaaag
     2761 ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt tccacaaaat
     2821 ttttcttaat actcgaaact aaacagcttt ttcacaacct ctccactaaa cggtattcaa
     2881 aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa catgaacagc tgtttttgct
     2941 tggctgttct aattacttga tgcagaacag ctttttcatc acctcttatg gataaattcg
     3001 gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg tcgataccag caatccaagt
     3061 gactgttcgt tcaaggccta gaccaaaacc agagtgcgga acagaaccgt atttacgtag
     3121 atcaagatac catgaatatt ctttttcatc caaaccgtga ttacggattt gttctagcaa
     3181 gaaatcatag tctgttgccc gttcgcttcc gccaataatt tcaccgtaac cttctggtgc
     3241 gatcatatct gcacaaatta ccacgtcttc tcttgttgga tgtggtttca tgtagaatgg
     3301 cttgatggct tttggatagt ttaggataaa gacaggctgt tcgaatgaat tcgcaatgaa
     3361 cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg tcatcaaatc catttttctt
     3421 cagtaattcg attgcatcat cgtaagtaat acgagggaat ggcaattttg tataagtttt
     3481 taagacttct ttgtctcgtc ctagtacatc taatgcatat tcgcaatgat ctaaaacatt
     3541 ttgtactaag aatgctacat attgttcctg tacttctaaa ctttcttctt gatgagtgaa
     3601 agccatttct ggttcgatca tccagaattc gatcaaatga cgtcgtgttt ttgatttttc
     3661 agcacggaaa gtcggaccga atgaaaagac ttttccgaaa gccatagcag cagcttccat
     3721 gtataattgg ccactttgag ataaataagc tttttgatca aagtaatttg tttcgaatag
     3781 atccgtagtg ccttctggtg cagaaccagt caagatcggc ggatcaattt tgacaaaatg
     3841 atgttgatta aagaattcat acgttgcacg aatgatttca tttctgatct gcataatcgc
     3901 atgttgtctt gaagaacgca gccataaatg acgatgatcc atcaagaagt ctgttccgtg
     3961 ttcttttggt gtgatcggat aatcgtggct ctcgcttacc acttccacat ttgtaattcc
     4021 gatttcataa ccaaattttg aacgactgtc ttcacggatt tctccagtta ctaacacgga
     4081 tgtttcttgg tgtagatttt tagctgtttg gaaaacttct tccgacactt cgctttttac
     4141 aactacacct tggaaaaagg ctgttccgtc tcttaattgt aaaaatgcga tttttccgct
     4201 tgatcgctta tttgcgaccc aagcgccgat tgttactgtt tctcccacat ggtttttcga
     4261 atcgatgatt tgaatttgtt ccacgtatga ctctcctttt atattccttg aatttcttca
     4321 ggattaattt tgacttttac tctcaataaa ttgcttgatt ctgcgtaccg cttcttctaa
     4381 cgtagcgcga tcagtggcat aactcatgcg gacattttct ggtgccccaa agccttcgcc
     4441 agtcacaagc gctacccctg tttcttctaa aagatcttct acccattgtg tgacattttc
     4501 atacttacac atgcgtagcg tttcttttac attaggaaaa agatagaaag ctccttgcgg
     4561 cttttcaaga gaaacacccg gcagttctgc cacaagcgga tataacgtat tcaggcgttc
     4621 ttcaaaggcg ttccgcattt cttctactgt atcttgctca cctttcaatg cttcaacagc
     4681 tgcatattga ctgactgctg tcggattgct agttgattgg gaagcaacag cgatcattcc
     4741 attgatgatc acaggatttc ccaccgcata accaatgcgc cagcctgtca tcgcatatgt
     4801 tttagacacg ccgttgatga tgatggtctg atttttgatt gcttctgaaa tcgttgcaat
     4861 tggagtaaac tcattgccat tataaactaa acggccataa atatcatcag caactatcaa
     4921 aatatcattt tttactgccc attcaccgat tgcctgcagt tcattcttgc tataaatcat
     4981 tccagtcgga ttcgatggag aattgatgat tacggctttt gttttttctg aacgtgcttg
     5041 ttctagctgt tcaacagtaa ctttccagct attggattct tcccctttca caaagacagg
     5101 aagtccttct gccagtttta cttgctcacc gtaactgacc cagtaaggca caggaatgat
     5161 gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta tacaaagcaa atttcgctcc
     5221 gtctgttacg actgtttgtg aaggttcata gacaagtcca tagtttttct tcagataatc
     5281 aacgattgcc tgtcgaagct ctgggattcc tgctgttggt gtataatagc tagctttccc
     5341 attgcggatt gcttcgattg ctgcttcttg gatgttttct ggtgtcgcaa aatccggttc
     5401 tcccacagtc aaactcagca catctctgcc ttttgctttt aatgccttgg cctttgccgc
     5461 agctgccaaa gtgacagacg gttctaatcg ttttgttctt tttgataact ccatcccatt
     5521 cattccttta ccttttaaat tcccttgatt gtcttgactt catctccaga ttcaaagcca
     5581 atcaggtaat aattcagttt cccatttgtt tcttttccta ctacttccca cacaggctga
     5641 tcatcataca tccctaaagc agctttttcg atttggattt caggatgggc cgcagcaatt
     5701 gtctgttttg cttcgttttc agtcagtccc tttgtttgat ccaatacttt tactttttca
     5761 ccagatttag gaatgataac ggcgatttct tttcctttat catttgtacc tgtcagtgaa
     5821 aagtatgtct tctttcgagt aaaccaataa aaatgatcgg tttcctcaag atttgcatat
     5881 ttctttgcga tttctactgc ttctttcttc gcctgcaccg ccggacgact ggaacgcaag
     5941 taaaaaatcg agctgatcac gatgaccgct aataaaaata cgatcaagcc aagtaacacg
     6001 cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg gtcgtcgctc ctttatcttc
     6061 attccttttc attatagcag attttaatta tgaaaaaaga cagctcctct aacttccttc
     6121 ttcatttttc tttaagaatt catggatatc ctgatagatt tcctccatag ctagctcttt
     6181 gatcggtact tttttcggta atgcatggat gatccgtgcg ccatattttg cagtgactag
     6241 tcttcgatcc aaaataagca atacgccacg atctgtctcc gtccttatca agcgacctaa
     6301 tgcttggcgt aacttcaaag ctgctttagg tacggattcc tggtaaaaag gattgatccc
     6361 ctctgattct aaataagcgt tcctagcttt gaccatcggg cgaagcggat tatcgaaggg
     6421 taaacgtgtg actacaacga tctgcaaagc atctccaggc aaatctacac cttcccaaaa
     6481 gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg aagcgtttca aaagcttctc
     6541 ccggcttcca ccaaaccctt gagctagtat ttctcgtcct ttttctagca tagacagatg
     6601 cattttctga tagaccttct gcaaaacatc atgagaagtg aataacacaa gtattggccg
     6661 atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa taatcgatat atgcttgatt
     6721 gctgacttga tggacggcag gtgcctcttt tggcacatag atccttgcct gtttctcata
     6781 atcataagga gaggcaatca ctttaagagg tgctgcttca atgccaagcc tcttagagag
     6841 atagtgtcga ttagggccga tcttcagcgt tcctccaata taaagaatac gatcatatcg
     6901 aggataccaa acagtcgacg gcaacatagc cgcttgaaaa tcatggagat gaaaaatcat
     6961 ctgctgcgct gtgttcccag gaacgaacca atggatcaaa tgatctcgcc agtcttccag
     7021 ccattgatcc atcagcaagt attgcttgtc taattgatca aataggctat ataaacttgc
     7081 taaatcgatc cgcatctggt ttgtccagct ttcttggata atctcaaaag tcagacgcat
     7141 ttgtgactgt agctgctgga tctcctgata aaaaagctgc aggtgatcaa tggctgtccc
     7201 agattccagt ggcagctgat ccaacatctc ttttgtcatc atgacttctt ctggatactg
     7261 tccgttaggt tgcttgccgc tcactaaact cattaatcct tctgccatac aaatctgtgc
     7321 ttcgcatagc gcagaaagtt cttctcgata aatcgataac cagcgtaagg cttcatgttc
     7381 tttcggtaaa tagctttgta ttcggtcaaa cagctgattt ggttcttcta attgctggat
     7441 ttgtttatgg aaatagttaa tacttaatcg tttgtcattt actttttcag cgatttccgg
     7501 caaatgatgt acttcatcga tcaacaaaaa tggactagct ggaagcaatg gttcttttcg
     7561 ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa aaattacttt gcctgatttt
     7621 cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac tgatttcttg ctaaaaatcc
     7681 aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc aaatttgtta aatgtaattc
     7741 atccaaatca ccagtcttcg tttgcgtcag ccaaactaag accgccattt gatacaaagc
     7801 atattgtttt tgttctggcg gttccacaag tgtgccttta aaacgctgca aatcaatata
     7861 gtggcgatag cttttgataa ttacaggttg tatgggctga tctagcaatt gattcagcaa
     7921 tggaatgtct ttttctatga tctgctgctg caaaactagc gaaacggtac tgatgacagc
     7981 gggtttttca ggtgtagcca aataactcaa tggcagcaga taaccgattg ttttacccat
     8041 tcctgtagct gcttcgatca tcaaatcttt tgattcatct tttgtgaaat ggtcgtagac
     8101 cgcattcatc agtctgttct gttcttttcg atacatcagt gttttaccaa acattttttc
     8161 ttttgcttgt ttttttcttg gatatgtccg ctcgccataa tggacggatg tgaatagttc
     8221 cacttctttt ttctgcaaag ctagcccatc gacgatttct aatgagggat caagtggctc
     8281 tggtttttct ttcatttctt caatcacatg ataaatgaaa cgactcgtat ccatacctgt
     8341 gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt ggcaattttc tcatccgttc
     8401 ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca ctatctgcct gatgaggatt
     8461 ctcatgaata aatccaaggc tttctgacaa atcagctaag cgaaagcttg gttccgtcgg
     8521 caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt atcttcaaac taggaagtcc
     8581 gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa taaatattat gcgcgacaaa
     8641 tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca tcttcgaaat aaggcgcttt
     8701 ttggacgcgt tgattcgtga tccctgttaa atgctggatt tgttttgaaa tcttccgccc
     8761 tggatgaata tcgatcgaaa aacgtgaagt gatccgacct gattcaatca tgacgcagcc
     8821 aaattggatg atccgatctt cttttggatt cgtcccagtc gtttcgatat caacgactgc
     8881 ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc tctaatgttc ttttgaaatt
     8941 atactacaaa gtgcagaaaa atactctcat cgctttttaa agaaaataga caaaaacgcc
     9001 cagacacgca aatgtctggg cgtttatttt ttagtatagg ttaattaagc tttagtaacg
     9061 tttgttgctt gaggtccgcg ttggccttct tcaacatcga aagttactgc ttggccttct
     9121 tctaaagttt tgaatccgtc gccttggata gctgagaaat gaacgaatac atcgtttccg
     9181 ttttctgcag tgatgaatcc aaatcctttt tctgagttaa accattttac tgtacctgtt
     9241 tccataataa aaatcctcct tgtgtatcaa cacatatttt gcaattactt gaatggtgta
     9301 tatctggaag atgtttattt gaaacaagct accacagatt accgaacaaa aactactcct
     9361 atagtatatc ataccgttta caaaaaagct agagtttttc gacttttttt attaaaaaaa
     9421 gttttctcag cgattacata aagtgaaaaa ccgaactttt ttttaaaata aacgacatat
     9481 tgttcagttg gtgttttttt gctctgatgt tcttttttaa actaaaaaaa ttattctttc
     9541 tgactactta tcggttctgt ttcaacggtc gaggctgggg aagattcttt agtaggtctg
     9601 atgttctcat ggtatttctg atagcctatg acagttacca gaagaacaag gcaaaccaat
     9661 acgaccaacg agattagttt ttcttttttg ctcatatcta tacctcataa ataacaggat
     9721 gatgtccatc atatttctga gtatgagtaa aagcaaaagc tgcagctgta gaatgtaaga
     9781 aatgccagca actttcataa tcacaggtcg gacagtacca aaagtaacca aatcttggct
     9841 gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag atctggtgtc tgtacataaa
     9901 catttttgta cagacgtgtg cctgtaatag gaaaccgctg tgtttcttca gcaaaaactg
     9961 gctgacttgt taaacttagg gatagagaca gcaaacacca taacagtctt tttttcataa
    10021 agacctcctt atcgataagt ttttcccaat taaaatcata tctcaagaac gcccgttatt
    10081 cacattttgt tcataaatga tgttttttgt atcataatcg tcaaaaagag gtcgtgacca
    10141 cgtcacaacc tcttttttaa acggtgttcc agaagtagct tcttcggaaa taagccgaaa
    10201 tttcacaaaa atttgagaaa taatttttgt caatctcttc ttatttcttg aagctgacca
    10261 cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    10321 caccaaacaa aaaatatgaa gagctatttt tgcttgctgt tcttattact tgatgcagaa
    10381 cagctttttc acaacctctc atacacggtg tttaatcagc tgaccttcgg tattagctca
    10441 aaaaacggca aaatatgtga aactattttt ccgtttgttt ctctaatact caggtcaaaa
    10501 cactgatttc acaacctctt ttaataacga agaatcgctg tcagactctt ttcatctggc
    10561 gcatcttttt gatccaaaat aaacactttt ccgccattaa tgatcacatc atctgctaat
    10621 gcattcaaca cttgacgccg atcatattct gtttcaggat cttcaccgaa accatcagct
    10681 aagtttgacg tagcgataaa taaatgtgac acttttcctt ctttcgcagc tggaacgata
    10741 tcaactaact gatcgatgaa tttcttatct aagagtttat tgtattcttt tgattcttga
    10801 gctgcgagtt cacttgacag cttcttcgcc ccttcttcga tatctctaaa ggatagctgc
    10861 gcaggagaag cagaaatcgc aacgtctgca caataatagg gatttttagc aatttttttg
    10921 aacatcgtct gattttctgg tagagcaaat aaataaatcg gccattgttc tggattttcg
    10981 aaagaatcct tcaagtacgt atcaactgct tggtaatagt tgacccaatc gatctctact
    11041 tcttcatctt ttggatttac gccatggaaa gttactccat ctcgggctcc gcctcctcca
    11101 ttagaagaga agttcaaacc gccacctgtc agttcagatc ccaatgcagt aacgacatca
    11161 accggtgccc cttctggtag agatacttct tttacttgtt tgttgctgac ttcatacaat
    11221 ttcatggagt cgcggttcaa tcccaataaa taataagaat agttgaactg cccatttttg
    11281 ataatagcca gtaaataagg aagatcactt acatagtatt gatcgtctac cgggatactc
    11341 aaacgatgga tataggtttg gtcagcagtt ataatgaccg ctacactcgt agttgcacta
    11401 cgccaaaacg aaggatctcc taacaaattt tcaatttttt cttggaacgg ggaccagtca
    11461 tgttcggaat attttttctc aaaacgtttc tttgcttctt tcgcaaaatt tttgaatttg
    11521 atctgatctt tttccacatc ttgatgggcg acatgcgtat tcagcatgat cgtgacgaat
    11581 ggtcctttag cgccttctga agtcaattgt gacaataatt ctttatcctt ttttgccata
    11641 aacgtacccc tcctaaaaaa atcattcctt tgtttctatc taaagtctac cacaacacta
    11701 tttttccata caataaaacg tatctaaaaa aataaaacgg tttctacggt tttaaattta
    11761 gctatttttt taagttatct tcataatttc ctcaattatt tagccccagt cttcaactat
    11821 tcttcatgaa atgctgataa actaagaaaa agaataaaag aggtggatct tatgcatttg
    11881 attatgattt tcagtttatt aagtattatc agtttatttg ctttcagcgc ttcccgctta
    11941 ttagatgcgg aaacacagcc tattcctgta aaagtacgta aaaacgaccg ctaaccttcc
    12001 ataacgtgcg ttcttatcct aaagtttttt cagaaacatt gaaactctct cccctctctg
    12061 ataaaatttt aatttgtagc gaaacaaatt aattacaagg agatcggata tgtccataaa
    12121 aaaaacacgt ctagtcctat tattggctag tctcattctt tgccttgctt cttttctacc
    12181 agctgttgct gtgcaagctg aagatacttt caaagtaaat gcaaaagcag cctttgctgt
    12241 agatgcgcaa agcggtaaga ttctttatga tcaagatgga gaaaaaacca tggggatcgc
    12301 ttcaatcaca aaaatcatcg gtctttacat cgtccttgat caagtaaaag aagggaaact
    12361 atcatgggat gataaagttt caatttcaga ttatgcagaa aatttgagtg tcaccccaga
    12421 tttatcaaat gttcctttgc ataaagaaaa cacctacacc gttaaagaat tattcgactc
    12481 tgcaatcatc cagtcagcca acgcttcaat ggttgccttg gcagaaaaaa tctctggcag
    12541 cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa gattggggta tcaaagatgc
    12601 aacgatcgtt aatgcatcag gactgaataa ctcttatctt ggcgaaaacc gaccagaagg
    12661 tactggagaa aacgacgaaa atcaaatgtc tgctcaagat gtggctatcg ttgcccgtca
    12721 tttgatcttg gattttccag aaatactgga tgtatctagc actacgacac aaatgtttgg
    12781 cgaaaacacg caatcaccag tagaaatggt caactggaac tggatgctcc caggattcgt
    12841 gaattataaa gaaggcgtgg acggattaaa aactggtacg actgaacttg caggagcctg
    12901 tttcgtcggt acgatcacta aaaacgatca acgaatcatc actgtcgtat tgaatgcaga
    12961 cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca gctcgcttaa tggattattg
    13021 ctacgacaat tggtcagaaa aagagcttgg aaaagccaat gcaagtatcc ctagtctaaa
    13081 aaccattgat gtaaaagacg gaaaagaatc aacagtgccc gttgtgctta aaagtcctgt
    13141 aaaagtatgg gtacggaacg atatggatac tggaaaatta acaatcacac caactcttga
    13201 caaaaaacaa gtcactgaca atgagatcca agcacctatg gataaaggaa caaaaatcgg
    13261 taccgcaacg atcaccttgg cagaagataa attaggttat cttgaagaca acaacgcgcc
    13321 atccacagaa atcatcacgg ataaaaccgt agaaaaagca aatattttcg ttattggctg
    13381 gcgacgcgtc gcagaatttt tcggtaactt gttttaattg aacaaaaaga aaaaagcatc
    13441 tttccatgtg gaagatgttt ttttgctttt tctagtttaa ataacagctt ttttcatcta
    13501 ttttcgttat actgtataag aaaaaggagt tgaatttatg aatctaacca gagatctgat
    13561 ttttttggtt ctctatctta ttggggtatt ctccatcgtt tattggcgaa aacagaaaaa
    13621 ggatccttcc ttgttctttt acagcagtac atggacgttt gcggtaatct tgacttattt
    13681 tgtcgtgttg gaaatttttt attcgacctt ttattttttg attcccttag tgtttttcag
    13741 tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg ttgaatggtc ttttattcaa
    13801 tattttcctc attagttttg gtatctactt gtttgtcttg ctatacgaaa cacaaaatat
    13861 ttttcttggc ggactgattg ccttgatcac gatcccgtta ctgcttgtct ttctattcgg
    13921 catttatgga ttgatcgttt ttcttttttg gaacggtgtc acagttttac gtagagagtc
    13981 gcattcatta gctaatcttt taacgttgat actagccatt tttcttacat tgtttcttgt
    14041 ttttgacttt ttcttgttaa aatatttacc acaatggata aatgctttgt ttttctgcat
    14101 cccattgatc ttgatctatc tatttatcgt attctacaac tttttgactg tctcattctt
    14161 gtatcagttc aaccgccctc gatacaacca agacttcatc gttgtgttag gcgccgggtt
    14221 gatcaatgga gaaacagttt ctcctcttct tgccaagcga atcaataagg cgattgcttt
    14281 ttattgggca caaagccgtg cgacattaaa tccgcctatc ctattaatgt cgggtggaca
    14341 gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa caatatgcca tggaacaagg
    14401 aattcctgaa agagatattc ttgtcgaaac caattcgaca accactttgg agaatatgcg
    14461 ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc tatcgcgcga tcttcagttc
    14521 caacaattac cacattttca gagctggtct ctatgcacga caagcgaaac tgaaggcaaa
    14581 tggcattggg gcaaaaacag ctttctatta cttgcctaac gcgtttttaa gagaatacat
    14641 cgccatcttg atgatgaaca aaaaaagaca tatgatcatt tgcggattgc ttttcgcagg
    14701 ttccgcattc ttatcactga tcactttact tttctaaaaa aacaaggtgt gacaaaacat
    14761 atgtcacacc ttgttttttt cttttattct cttgacgatt ggcaagaata gtattccttt
    14821 tttgacggct acttgctgtg cagaataaat accgctgttt cctgaacaca taaagctgat
    14881 caagcagatc ataaagaagt aagcagcagc ttgtgaacca aataattcga ttcccatcac
    14941 aaaacaggca atcggtgtat ttgtcgcacc agaaaatacc ccgataaaac ctaatccagc
    15001 aagaaaagga acagaaacat ggagcaaagg cgctagtact gcgccaagcg ttgcaccaat
    15061 ctcaaatagt ggtgtcactt ctcccccttg gtaccccgca cctaaagaca agaccgtgaa
    15121 gaataatttc ccaataaaat cataagcatg tgcctgtcca ttaaatgatt cctgcagtaa
    15181 cggaagactc aacccaaggt aacgttgtgt ttgcaaaatc aacgctgcgg ctacaactac
    15241 tgctccacct atcgtatttc gccaaacagg atgaggaatc cattgcgcat accgcgcttt
    15301 taaaaagacg atcgaacggc tgaataccca accaatcaaa ccaaaagcaa tcccagcaac
    15361 aaaaactttg aagaacagca acatactcca ttcaggaatc acacccattt ggtaatgtag
    15421 atgagaaaca ccatagcttt ctgtcatgaa atttgcaaaa agcgcagcaa agaagctagg
    15481 aaatattgct tctgttcgga ctttaccgat tgccaacact tctaatccaa ataatgttcc
    15541 agctaaaggt gtgccaaata cagaactaaa acccgcactg attccactga taaccaaaat
    15601 ctcccgttct gctttatcca atcggaataa atgcgctata ttatcagcta atgctccccc
    15661 catttgaact gctgttcctt cacgaccaac agatccccca aataaatggg tagtgatggt
    15721 cccaaacaag gtcaatggaa tgagccgcaa agggatcttc tcttctcctc cattcccttg
    15781 atcgatcact aagttattcc cacgactaga aagaccgcca tgatacgcat aaagataagc
    15841 aaaaagcgcg ccgctgactg gcaatagata aagtagccaa ggatgtgcta gtcggatatc
    15901 tgtcacgaac gtcaggctat ttaagaaaaa agcagacaag ctccccatga atactccgag
    15961 aatcgctgtg accaccagcc acttcaacat gtacatcaaa atacgcactg gactcggtaa
    16021 ttgtttttcc ataaaaaatt cctcctcaaa taaaaaaacg cctactaaag aaaggggctg
    16081 gccccagact ttagtaggcg tcattagcta tcttacgtta gcatttaaag gcgaacacca
    16141 tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa taaaattatc
    16201 gttatttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt aactgttgat
    16261 tacattgatc actatcatga caaatataat tccccttttt cgtatacgtg ccatcagaac
    16321 cggctttcgt tgtagaaaga aagagcgaga cattcgatgt tttctgacag atcgcacaga
    16381 ttcccttatt aatcgttggt gataaagtac cgctgatccc ttttaatttt ccatcttcgt
    16441 aataaaccat gaattttttt tgcgtccctg gatcattcca accgatgtac gtatgtgctt
    16501 gcaagtcgat ttcattccaa gcgggttgtt tgagtttttt cacttttcgg aataatttct
    16561 cgatttgtgg tttgctcggc tgcataaacg gctggacgat ttgcttcagc tcctccaatg
    16621 ctcgttctgc ttgcgttttt gtcagtttta catctaaaac tgtcgttaaa aacgtatcga
    16681 tttctgatgg ttcgtaacct aatacatcgc gaatcttttc agctgctaga gcctgaaccg
    16741 tttgtaccgt ttttgagtca ttgacagatg agtacgcatt taataactgc tctacttctc
    16801 tttttataaa aaaatattga tatggtttca ttgtttgttc catgatttta aagtcctttc
    16861 ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta tgtgttacct
    16921 gaagatcccg gactctttta aataagatac gttttgcatc ttatcatcac cccctttaat
    16981 gttcattctt ctatgtacat gattcattcg ataaataaaa gtaaaccgta tcttgagcag
    17041 atagtcaact gcaaacagaa aaaggactgt gacaaaagtc gtgtcaaagt ccctttctac
    17101 aaacaaacgg tgttccagaa gtagcttctt caaaaaataa gccggaattt cacaaaaatt
    17161 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgacccact tctgtcacaa
    17221 cctcaatatg gtctattatt ggttttacct gttaaatcga ggattctttt ccgaagtaac
    17281 gttgccatct cttacgcata cttgctgaca taggctgagc ggcttctttg actgcgcagt
    17341 atttgtctac gaatgttacg atatatcctt ctttgtactt aggcggtgca atcgttgcgc
    17401 cccacatgtg cttcaaaatg atatctcgct caagatccga caaatcggtg atcttttctg
    17461 cattctttac agcgattctt ggatggatat acgcatgtgt gccttcatca aatttcgtcg
    17521 tacgccaatc atagtagaac agatcatgca aaagtccagc acgcgctgtt gctttagcat
    17581 tgccacccca tttttttgcg agcagataac tatagtaaga aacactgatg gaatgctcca
    17641 aacgagtgga atgaacatgc tgtgtgtaat tcgctaattt tttcactgct tctgtttcta
    17701 aaagatcttc tacataagac atatattctt cgtcttcacg ccatttttct gtcataatta
    17761 tcgatctgcc acctttttga atttcatttt cttctgaaat tgatctaagt ataccaccag
    17821 tgttcggatt attctagtcg ccttggataa aatagccaaa gcttaatagt tccttaatca
    17881 taaagaaaaa tgataaagaa caatccctgc cgcaacccca acattcaacg attccgcatt
    17941 tcctttcatc gggatatata cgttctgatc agtcaaagac aagatttcct gacttacccc
    18001 ctgaccttcg ttccccatga ttattgcgaa gttatctgat ttttctactt tgtctaaggc
    18061 gtgtgcttct tcatttaatt ctgtgccata cactgtaaac cctttgtctt tcattaaagg
    18121 aatcacttct gtcaaatctt tttgcaacac aggcaaatga taattgcttc cctgcatgct
    18181 gcgcagcacc tttgtactat aaatatctgc cgttcctttg cctaataaaa caccagccat
    18241 atcaaatgca tctgccgtac ggatcatcgt ccctacattt ccaggatctt gtacattatc
    18301 taataacagc catcctcctg aaaaatctgg cgtgccttca gccgttaacg ccatcactgc
    18361 taagattcct tgtggtgtag gtagatccga tacagcatcc atgacctcat ccgaaactag
    18421 aaaagtagaa atctctttgt ttgcggccag ccagtctccc cattctttca tgcctcgctg
    18481 tgtcacaaaa agttctttta tttctgcatc agacgacgca gcttcttcaa tcagatggaa
    18541 cccttccaaa agatattctt ccttttcttc ccgatatttc tttttatgta gtttttttaa
    18601 ttctttgatt cttggatttt ttgctgattg gatctctttc acattcctca cctccccatg
    18661 attatatcat ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattaat
    18721 ttataaaata ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac
    18781 gcgtccaagg cgtaggattt cgctacatga ccaagatggt cgctgatcaa ctaggcgtga
    18841 ctggaacggt aaaaaacgaa gatgacggct cagtcacgat cgaagcaatc ggcaatgacg
    18901 acatcatcca gaaatttatt gaagaagtca agaaatctcc gagcccaagt ggacgagtgc
    18961 agtatgtgga tgtccaagaa gacccgatga ttgaggaacg aaagaaattt gatgtggtgg
    19021 gataaggttg tgaaaaagct ggtctgcatc aagcattaag gacaggttgt gaaaaatagc
    19081 tctccatatt tttcaacagg ctggacttaa tgccgtagat gcaagctttt gaacaccgtc
    19141 aataaggttg tgagaaagtc gcttagtttt gagtagtaag gagaattttg tgaaaatggc
    19201 tttccacatt tttgcataaa atttgactta ataccgagaa actgactttt gaacaccgtc
    19261 gataaggttg tttttgagac gctttgcctc aagtattaaa gataggtagt aaaaaagctg
    19321 atctataact tgcaataaat ttgcttaacc catattttcc agacagaaca aaaaagtttt
    19381 tagtttttta aataagaaag aaacacttgg aatcactctt ttctttccaa gtatttcttt
    19441 cttgattttt tgtctttttt tcaaaatttt tcgctacgta aaagaacgaa tatttatatg
    19501 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
    19561 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
    19621 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
    19681 gacacttcgg gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
    19741 ctagttgaga atttcaattg gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc
    19801 cgtatcatca tcttgccact aggtattagt caatcgaaaa aaacaatgat ccaatctgaa
    19861 aaaatgcaag cgttaaaacc acaagttgaa gcagcccaac aaaaattgaa agctgcaaca
    19921 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtataccg cgaaaacgga
    19981 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
    20041 gccttgtact ttacggcacg ttatacagaa ggtattcggg aatcatcttt ctacggcatc
    20101 gatttaggct caccaagtat ggtacttgtt gtcattgcag gggtcgccta tctgcttcaa
    20161 ggatacatct caacgatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgcta
    20221 atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg cgccagccgg tgttacactt
    20281 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
    20341 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
    20401 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
    20461 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
    20521 cagaagcgga aataaaaaca taggacaaaa aagatgagag ctaagccctc atcttttttg
    20581 ttactcttcc tcttctgcta ttccattaat taattcgttg acagagacaa tgctttcaca
    20641 tcctgcacga acaaactcgg gctctgaacc aatcatttgt gagttgataa attcaatgac
    20701 catcggaaga ttcatcccag cataaagagt aatctgttgt ccttccatta ttagtttaga
    20761 aatgatattt gcaggtgtac cacctaaaag atcgcatagt accacaaaat catttaacga
    20821 atctgtgaca gtgagaaact tttgtttgta tgtttcagtg ccatcttctg gtaataaagg
    20881 taacgtgtag atatcctctt gaggccccat gatcatttct gtactttttt tcaattcctc
    20941 acaaaatttt ccgtgactaa tcaataccaa ttgtctactc atttttcgct ctcccttatt
    21001 tcgaaatcac acccaatgca gataaagcaa cacacacaat taatacgata aaaatcgctc
    21061 gagtaggagt catgtttttc tttcctaata accaataaac accaccgacg atcccagcag
    21121 gtaaaatttt aggtaagatc atatcgatat agttttgcat attcaatgtc acatcgccaa
    21181 tcgttggtgc ccatgaaaat ttaacattga tcattgtcgc aactaatgcc cctaccatga
    21241 atacccctaa taaagtggct gcgtccgtta atgcagttaa gcgagattgc atagtagtaa
    21301 ctaaggaaat tccttctttg tatgcaaatt caagttgctt ccaacgaaaa accatgacaa
    21361 cgatctgagc aaaaatccat ataaacacac ctatcggatt tccatcagtt gccatattcg
    21421 ccgccaaagc cccaaaaatc gttggaatca aagcaacaaa tacggaatct ccaatcgctg
    21481 caaaggaacc catcaatcca gctttcagcc ccgatacggt ttcctttgaa gcaattccct
    21541 ctttttcttc cattgcaaga tcaatcccag taatgattgt attaaaaaaa ttagaagtat
    21601 taaaaaattg cgtatgtgtt ttcatcatct cttttaattc tggtgtattg tctccatata
    21661 tttttcttag ttgtggcaag atagtatata aatagccaga accctgcatt ctctcatagt
    21721 tccaacctaa ttgaaaagtg aacaaacttc ttcgattgat ttgcttaaaa tcttcttttg
    21781 ttaatttgta attagatttc gtcatcttcg atttccccct caacagaatg tgatggctca
    21841 ctagctaatg gttgatggtc tttgttttga tcattcttat aactgatcat tgctagtgct
    21901 agaccaatca atgctacagc aagcatcggc agcgaattga aagttgccgg aagatcttta
    21961 acaacgcttg ccacacttgt tcctaataat tgaacattag agaataacgt tgctaataat
    22021 gccgtgatcg taaatcctaa aataagataa gatagatgtt tttttactgg taaatagcgc
    22081 agaagaatgg caaacccaac agctggtaaa acagctcctg ctactgaaag tccatcgcct
    22141 agccatttta gattcccgtt taaaacagaa acaacatttt caactaatcc accaccaaat
    22201 gctaaagcta aaaatacagg aatcatacga gagagcgacc aaggtaaagc tcccattaag
    22261 aaattgcgtt cgattgcttg ataattcatg tcttctacgt gacggtcaat acgatgggca
    22321 aagtatgtgt tagcgaatct agctaaaata tccaattgga tcattaagct tgctacaggc
    22381 actgcaatag cagcaatcgc ttgttctgga ttcattccaa gagaaatcga gaaggcagtt
    22441 gctaaaatcg tcccagagtt ggcatctatt tttgatgcgc ctccaaacgt ccccactcct
    22501 aaaactgtca actgcatact gccaccaata atcaaacctg cgtttaaatc acccattact
    22561 aaacctgcat ataaccctgc aaacactggc gcactcattg aagaatagat ctgtaattca
    22621 tctaaaattt gataacctgc atataatgtt aataataaga tctgccacca ttgaatatcc
    22681 atggatcatt cctcctattt gattaaaaga ttcataaagt cttctgcttt atcgtttggt
    22741 accatttgag caattaaatg tataccaagc tgatctaatt ttttaaatac ttcgatatct
    22801 gcatctacca cattgattga ttttgtaatg gaccgtgttt cattcgtttg tgacatattc
    22861 cccacattta cttctttgat aggaactcct ttttctacta aagataagat tcgatctggc
    22921 ttttttgcta cgatcaatac tcgttgagag tcatatttgc cagctaaaat atttgttgct
    22981 gctttttcga ttggcaaaat gcttaatttt acccctgctg gagtcgctaa cttcaatcca
    23041 cttttttcaa ttgtgtttgt ggctacctca tcatccacga ccatgattcg actaatattc
    23101 agtttcgttg tccaaagatt tgctacttgt ccatgtatca aacgtccatc gattcttaca
    23161 ccaataattg ccatcatgca ttctccttat ctattttttc tatttattgt tcaattaaaa
    23221 ctgtgacatc atcaaatatc ccttctgttt caaagataac gttttcattt tttctttctt
    23281 atatttcatt tttaagctaa gaacaaattc cttcccaact aaagaaaacg ctttctttta
    23341 tatttaagtt catttgattt tcttaacttg aatgtgtttc atcaacatcg taactttcat
    23401 agggtcgatc cagccagttt tctcttgttg cgcgtttgcc ataccacaag ccatagaaaa
    23461 tgcgagaatc tcttcgattg attgcctttt tgataacgca tgggccgatc ctgcaagggt
    23521 cgcgtcacct gaaccaacag gattaacaac tgatatttta ggaataatga cttcgtaaaa
    23581 acactgagca acttttgcca cagcacctct ctctcctaaa gaaacaacaa cccatggcac
    23641 tccttcaaat attggatgag ctaataagga ctgaattttt tctaattgaa tcgaagaaca
    23701 gatacgttgg ccaatcagtt ctcctaattc ttccagattg ggcttgataa gtagtggctt
    23761 ctccttacct tccaatactt caagcaaaaa tttaccagaa gtatctagta atatgtcttt
    23821 ccctttttta tcagctaaag caaccaattg cttatagtaa tcaagagggc atccttgggg
    23881 taaactacct gagatcgtca taatatctga tatttcgagg agctgctcga aatgatcggt
    23941 aaatgtctgt tgttctttct ttgttatgaa ggggcctcgt tccaaaagct ctgtttgtct
    24001 cgaactatgc aggatagcaa tagaatccct tgtctctcct ttaattggaa caaactgatg
    24061 catgatccct aactgatcta gctggttctt tagatattca ccgtgctggc cacctattaa
    24121 accagtagct atcacctctt ctcctaataa atttagaacc ctcgccacat tcactccttt
    24181 tcctcccgct gttttattga ctgtttcaac tcgattgact ttgtcgaggt ttaattcttc
    24241 aattgtatac gcgatatcga tggaaggatt caaggtaact gtcgtaatca tttcatcttc
    24301 acctttttgc tttttgtttt attgaaccaa ggggtagcgg tttcatttat tactttattt
    24361 aaagtctcga tgttttctct cccatttgtt cttaaccaat gaatagctcc gtcttttcca
    24421 ttttcgacgt atgctttgac cccatctttc catgtcgcac gtccacacaa tacaccattg
    24481 aaggtggaac ccgattcatg agcaaaaatc agtgtttgtt gaaataattc tgttgaaaca
    24541 cccccactca aaaaaatgaa tggtaatgga gtcgcttcac tttgctcctt aaagaaggca
    24601 gcagcctctt ctttgctgtg aataaattca cctgtggaat agccattcac ataattcata
    24661 ttgactggta cttctacttt caacacatca actttatatt ttggatcaga aaattctttc
    24721 atcatctcaa taactttgtg gggcttgact ttcgcatact ctggagaaga actatcgctt
    24781 atttgtgcat cataagaaac taactccaaa tagaagggac gattttctcc ttcgcactca
    24841 ctacctaaac gttcgatgaa cacatgcttc aaatgattga tttcgggtgc ttcatcaaca
    24901 tcgtagtaaa gtaagaattt tactgcatcc gcttgctgtt ctttcaatct gagagaagac
    24961 caatccgcta ataaatcagg tagacgacca ggcgtagttg cgtcatagcc ggttttctca
    25021 taagctaaca ataaccccgt ctcttttgct ctgacttttg ctgctggcaa tccatattcc
    25081 ggatccaata aaatagcaga agcatagggt gtcagctcag ctgatactag ttctttgaat
    25141 tcactgattt ggtcttcact tggctctttt cccaacgatt tgatcatttt ttttagagca
    25201 ccacgctgat cgatcgctaa tgcactaata atcccatctt ttgttgatag tctctccatt
    25261 gccctatttt tttcttcatt catctgtcat ttctccttcg gtttcatact tatgaataat
    25321 gacgcccttg actactcgat tgaccgtgcc tgttggagat ggtgtatctg gtttattagc
    25381 tactttgatt gaagtataga gtgcaatgat ttgtgcagtc ataatcatcg ctaatgcaag
    25441 ataggcatcg ggcaatttac tcaccgagtc ttccaatagc acagtcatac ctgagaaatc
    25501 aatagtaccc ggttgcgcaa tcgcaatgac attggcagtg acccgatctt cttttatttc
    25561 ctctaaaata tctaagtcat aatttcgcgt atatggttga ttgctcacaa aacctacaac
    25621 gagtgtctcg ctatttaaaa aagactttgg tccatgacga aaccccatcg aagaatcaaa
    25681 aattgtagca actttacctg ctgttaattc taaaattttt aattgtgctt ctcttgtcgc
    25741 acctgccaaa cttcctgaac ccagataagc gatccgttca aaaaaatcag gtaataactt
    25801 ttgaatttct tgatctcttg aaagaagatc ttgaccgatt ttaactaatt gttcaagata
    25861 tctttctttt ttctccattg ctgtttgatc aaagatccat aaagcactca ataacataca
    25921 cgtaaaactt cctgtcatag caaaacctgc atcgtttgct ctctctggca tcaataacag
    25981 ataattctgt ttatcatcct gtgcgttttt tgccaacatc ccctctttag cacaagtaat
    26041 cgtcaaatga aacaatgttt caacttgttc atttgcaatt tgcacagccg ctacactttc
    26101 tggactgttg ccactgcgag caaatgaaac taataaagtc ggttcatttg gaaacaaaaa
    26161 ttcttttggt gaagctacaa tatctgtcgt gccaaaactg tggaagataa aaaactgatt
    26221 gtctccatgt tcatttagat agggtaagag cgtatcccca atatattgag acgttcctgc
    26281 acccgtaaaa ataacacgta ttcttcttcc gtttgcttct ttttttattt gatcaaaaaa
    26341 tagagctaaa ttttcttggc tttgcatcaa tctatcgatt gtttctttcc agagatctgg
    26401 ttgttgtttt atttcttgtg tcgtgattgc tgcacctatt tgttttaatt tgttcttcga
    26461 tagctcaaac atgttgttct ccctctcaat cattttcgga tatgccgaat tttataatga
    26521 aactggtccg ctcttgctac actatctgta tattcgataa tctcattttt cacgttatag
    26581 gttatcctcg tcagatgtaa gacgggagcg ccatcagcga ttcgtagcac aacagcatct
    26641 tttttttgca cgatacttgc ataaaactct tcatcagcta attgaacttg ttgttcataa
    26701 tcctgtgaaa acacatcata caacggttta ctttcaagta acggcaaatc aagtcccata
    26761 aatagtttcg ccggtagata agaacgctca accatcaatg gctcttcatc tgccaaacgc
    26821 aatcttttaa gtttaaatac agtttcacct aaatggatgt tcaaattctt agcaattctc
    26881 ttattagctt ccatttgtct aaattctaaa atgaccgtct tagggacgcg ccctatttcg
    26941 cgcatttctt ctgtaaagct atatgctcca gctaagttcg atgacttatc agacaaatca
    27001 gagacatagg taccttttcc atgtcttcgg taaatataac cttgattttc caattcctgt
    27061 aaagccagac gtacggtggt acgactcacc ccatagtgtt gacaaagttc tctttcagat
    27121 aacagcttat catgcggagt catctgtgtt tcgatacgat ccttcaataa atcaactagt
    27181 tgataataca atgattttcc tttatttgag ctctccataa aaatcacctc ttctttactg
    27241 gttataacca caacacaaga atagtgaatt attaatcatt tgtcaaaggt tttttagacc
    27301 aaaaaagcta ttataacagc tctttaaagc tttatatctt gaatagacaa agatacctca
    27361 aaaataccag ttaccaataa aagaacagaa aaaaagacga accacataag cgatccgtca
    27421 aaatggtcta acttatcgag cagcgtgatc cacattaatc cctatgatat tcctacaact
    27481 atcgttttat tacttccaat atcatttaca acaactaaaa atagcttatt aatgacaaat
    27541 gttattatta gttttagata acttttgcag ttttatactt tttttctata aaatttgaca
    27601 aacatttttt taaataacta attatttttt gaatctgtca ctcagacaat cctttctctt
    27661 acttgttaac ataaatagaa gttgctgttt cttctaatag tgtacttaat tctttaatat
    27721 tttgttttcc ttcttctaac atccaactac tcgctttttc ttcgccttcg gtaacaaaag
    27781 gtgctactcc cttcgcccat gttgctcgcc cacataagac accattgaat gtcgaacctg
    27841 attgttttgc aaaacgcaat gtatcttgaa atagtgcagc gctgactccc gcacttaaaa
    27901 aaataaacgg ttgttcagtc actttcgatt gttcatagaa atacgcggat gcctctgctt
    27961 gactataaca agtatcttgc ccatatcctt ccacaaatct catattcact ggtacttcta
    28021 ctttcaatac atccacttga taacgttctt ttgaaaattc tcgcatggct tcaatcactt
    28081 tatgcggttt tacttttgca tagtcaagag aagacggatc gccaatagtt gcatcgtaag
    28141 aaaggagctc taaaaagaaa ggtatgcctt ctgcttcgca ttctgaacca atccgttcta
    28201 taactatttg tttttgcaca ttaatctcta ctggttcatc aacgtcatag tacaataaaa
    28261 atttacaagc atccgcaccc acttctttta accttttcac tgaccaacta ttcagtaaat
    28321 ctggtagtcg gcctggcatt ttcgcatcat agccggtttt ttcataagct agcaacaaac
    28381 cacattcttt ctctcgaagc gctgctgcag gcagaccata ttctggatcg agtaaaatag
    28441 cggaagcata cggagtcagg tactgagaaa ccatctgttt aaattggatg atatcttctg
    28501 atgtagcttc tttatattgg ttgatcattt tcttcaaagc accacgttgg tcgatagcta
    28561 gcgcgctaat aattccagct tcatccgtca atcgcttcaa acgttcctgt ttttctatac
    28621 tcattttctt catatatctt cacctatttc tttagttaat attgtagcta tttctatttt
    28681 tgattttgca gctctcaact gttctgtaac ctcttttcgc atcaatgatc gtgcaacgag
    28741 agacaataat ttcaaatgag tcgttcctgc ttctgaatct gggataaata acgcaaaaat
    28801 aaaatcaact gactgtctat ctaaactgtt ccattcaatt ccttttttca gtgtgacgat
    28861 aatcaaacta ggttcattga ttgtttgaga cttcgcatga gggatagcaa aacctgacat
    28921 tattcctgta gtaccttctt tttctctttc cagaagtttt tgatatactt ctttttcgtc
    28981 atgagcgatt cctaaacaga atgtttgctt tgctaaaaac ttaaatgctt cttcttgatt
    29041 atctaatggc tgttgtaaaa aaatgttctc ttcatgaatg attgtcatga taaacctcct
    29101 gctcttcctt cttcgataac tgtacgattt gttgctttat tatccctctt cttgtcttta
    29161 tcagttactc ttttcttttg gatcaaacct agtatcactc caccaacgat cgaaccaata
    29221 agaatcgctg taacccattg gatttttccc gtcactacag gtaacactaa aaaaccgcca
    29281 tgtggggctg gtacttggac accaaacaga taggtcaaga cagcagcaat tgatgagcct
    29341 aacatcatcg ttggtaatac tcggatcggg tctttcgcag caaacggaat agctccttcc
    29401 gtaatatgag ttgatcctaa aataaaatta accaaacctg cattacgatc attagtatca
    29461 aaatatttac caaacaataa agtagcaaaa ccagtaatca acggtggtgc aatgcaagct
    29521 gctgacgcac cagccataaa agtcgtgttt ccttgtgtca aaagagctgt tcctgttaca
    29581 tatgccgcct tattaaccgg tcctcccata tcaaacgcac acatacagcc aacaatcacg
    29641 ccaagaacaa gtggattcga atattcaaat ccagctaaaa agctcatcat tccttcattt
    29701 atagccttca ttggacctgc cagcaaagac atcacgatgc caataaatgc tacaccaata
    29761 actggatata agacgattgc cttcaagcca tctagttgtt taggtaaaat tatcagtagc
    29821 ttttgcaaaa ataagataaa ataacctgct aagaacccag ctaaaatacc tcctaaaaat
    29881 ccagtaccgc cattcatggc tacgattcca ccaacaaatc caacgactaa ccctggacgt
    29941 ttcgcgatcc cttctgcaat ataagctgct aaaactggaa ccatcattcc cattgctgca
    30001 tcgtcaactg cttttaccat tgccgctgta gcattgtatt gtgcatgatc tggatcagaa
    30061 gaatagattc cccaaacggc aaatgaaata gcaatcaata ctccgccagc taccacgaaa
    30121 ggcaacatat gagagacacc gttcatcaaa tttttataga tgctacgacc aaaattagca
    30181 ccacttatcg cttctggttc taaagtatca gcagcttcat tatctgctaa gacagtacct
    30241 tttcctgcca atgcttcgtc aatcagccgt tgtgcatcct tgattcctgt actgacagaa
    30301 acatcaatga ctcgttttcc cacaaatctt tctgaatgga catctttgtc tgcggcaata
    30361 atgacagcat ctgcctgttt gatttcttca gccgttaatt cattctcgat cccaatttgt
    30421 ccatgggtct ccactttgat cgtgatgcct tttttcctag ctgcttgctc taatgcctct
    30481 tgcgccatgt aggtgtgcgc aattcctgtc ggacaaccgg ttgctgcaat tagttgatat
    30541 tttcccatct ttcgaacctc tcttccttga ttttgatttg tttacttaat tcctccacat
    30601 ccaaaaaatc agtcagtcct ttacgaaaag cagtagagct gcctgctgct atgcttttac
    30661 gtaacgtttc atcaagcgat cgtcgattca tgtatcctgc taaaaaagtg cctaacatgg
    30721 catcacctgc acatgctgta ttgacaactt ttccttttgg cgagtttcct cgaatcaccc
    30781 tgtctttcgt aaaaagaatc gctcctttct caccgaggga taacaaaaca ttttctgcgc
    30841 ctgactctac tagtcgttgt ccataaacaa agtaatcttc atctgtaacc atttttcgtc
    30901 cgaaccaaga agaaagttct tcctcatttg gtttcagcaa aaacggtcga tagggtaaac
    30961 aatccatgac ttcttgatca ctactatcaa taatcaaaca aatccgactg atttctatta
    31021 aaacctttgg agacagccct cttggtaaac tcccagaaac acaaagataa tcccctgctt
    31081 gtaatgctct tatttgattt aaaaaattat gaacagctgt ctgcggtatt tcaggtcctt
    31141 gattcactaa tttgtattct tctcccgttt gattgacttg tgtaaataca ttgatacgcg
    31201 tcataccagg cacttcaata aactctgttt ggatttgttt ctgctgtaaa tagtcttcta
    31261 tatactttcc tgtgaaccca gcagaaaaac ctaatgccgt acttgggaaa tctagcatct
    31321 ttaatactag tgacacattc accccttttc cgtttgcctg aatgtcatcc tcttttgtgc
    31381 ggtttaccat aaaaggatgc atctcatctg tttcaataaa tagatcaatt gccaaattca
    31441 tcgtgcatgt gtagatagac ataaccaacg acctccttga ttctattatg aaatacgcga
    31501 aatagaaaat gttttcttaa tcgtcccaac acaaagaaaa cctttacatt tataaactcg
    31561 tcgtgctttt tttaactaac ttttggtgaa agaaggaaag aatatgaatc acaaattaag
    31621 tgaaacagag cagtacctct ggaactttat cgaacacctc atcctagaga tccctaacta
    31681 ttccatcgtg aaactaagtg aacaagcaaa tgtctcaaca gccaccattg tgcggacaat
    31741 gaaaaagaaa ggctatgaag ggtttacttc gtttaagcat catctaaaag aaggagaaaa
    31801 caagaacatc aatttttcat ttttagataa agtagacaaa ggaatccgcc gagccattct
    31861 aaaaaatgaa caagaagtgg tacgtacaat caatatgttg gaaatcggga acattgaaga
    31921 tgcaatacaa agaatcaaat ttgctgaccg ggtttttgtc tttgcccgag gattttcaga
    31981 aatgattggc caagaaatgc tggtaaaact tcaattaacg ggaaaaaatt gtcagatgca
    32041 tactgatcca gaaattatca aaagcatcag tcggaaacta actaagaaag acagcgttat
    32101 ttttgtttct ttgaacggtg agaaaacaaa taaaaacatc aaaaataaga tttatgtctt
    32161 tatttttcaa ttttttaa
//
LOCUS       DAJSBC010000027        31245 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSBC010000027 DAJSBC010000000
VERSION     DAJSBC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.027"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            202..1077
                     /locus_tag="ONT65_001330"
     CDS             202..1077
                     /locus_tag="ONT65_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328973.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ONT65_001331"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ONT65_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ONT65_001332"
     CDS             complement(1642..2973)
                     /locus_tag="ONT65_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2328974.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ONT65_001333"
     CDS             complement(3122..3835)
                     /locus_tag="ONT65_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2328975.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ONT65_001334"
     CDS             4010..5434
                     /locus_tag="ONT65_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2328976.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ONT65_001335"
     CDS             5733..6440
                     /locus_tag="ONT65_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2328977.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ONT65_001336"
     CDS             6427..6750
                     /locus_tag="ONT65_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2328978.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ONT65_001337"
     CDS             complement(6839..7876)
                     /locus_tag="ONT65_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2328979.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ONT65_001338"
     CDS             complement(8197..9180)
                     /locus_tag="ONT65_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR2328980.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ONT65_001339"
     CDS             complement(9595..10365)
                     /locus_tag="ONT65_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328981.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ONT65_001340"
     CDS             complement(10358..11293)
                     /locus_tag="ONT65_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2328982.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ONT65_001341"
     CDS             complement(11609..13258)
                     /locus_tag="ONT65_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2328983.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ONT65_001342"
     CDS             complement(13375..15978)
                     /locus_tag="ONT65_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2328984.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ONT65_001343"
     CDS             complement(15990..17039)
                     /locus_tag="ONT65_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR2328985.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ONT65_001344"
     CDS             complement(17065..17379)
                     /locus_tag="ONT65_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR2328986.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ONT65_001345"
     CDS             complement(17395..17841)
                     /locus_tag="ONT65_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2328987.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ONT65_001346"
     CDS             complement(17842..19764)
                     /locus_tag="ONT65_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR2328988.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ONT65_001347"
     CDS             complement(19976..20872)
                     /locus_tag="ONT65_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2328989.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ONT65_001348"
     CDS             complement(20865..21545)
                     /locus_tag="ONT65_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2328990.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ONT65_001349"
     CDS             complement(21546..22328)
                     /locus_tag="ONT65_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2328991.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ONT65_001350"
     CDS             complement(22321..23238)
                     /locus_tag="ONT65_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2328992.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="ONT65_001351"
     CDS             complement(23402..23725)
                     /locus_tag="ONT65_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2328993.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ONT65_001352"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ONT65_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR2328994.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ONT65_001353"
     CDS             27853..28167
                     /locus_tag="ONT65_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2328995.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ONT65_001354"
     CDS             28180..28554
                     /locus_tag="ONT65_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2328996.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ONT65_001355"
     CDS             28564..28899
                     /locus_tag="ONT65_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR2328997.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ONT65_001356"
     CDS             28982..30322
                     /locus_tag="ONT65_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2328998.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ONT65_001357"
     CDS             30400..31074
                     /locus_tag="ONT65_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR2328999.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ONT65_001358"
     CDS             31067..31231
                     /locus_tag="ONT65_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329000.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         9736 a         6069 c         5515 g         9925 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaat
//
LOCUS       DAJSBC010000028        30254 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSBC010000028 DAJSBC010000000
VERSION     DAJSBC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.028"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            33..257
                     /gene="nrdH"
                     /locus_tag="ONT65_001359"
     CDS             33..257
                     /gene="nrdH"
                     /locus_tag="ONT65_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR2329001.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            416..2575
                     /gene="nrdE"
                     /locus_tag="ONT65_001360"
     CDS             416..2575
                     /gene="nrdE"
                     /locus_tag="ONT65_001360"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR2329002.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2625..3590
                     /gene="nrdF"
                     /locus_tag="ONT65_001361"
     CDS             2625..3590
                     /gene="nrdF"
                     /locus_tag="ONT65_001361"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR2329003.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3679..4818)
                     /locus_tag="ONT65_001362"
     CDS             complement(3679..4818)
                     /locus_tag="ONT65_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2329004.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5127..5840)
                     /locus_tag="ONT65_001363"
     CDS             complement(5127..5840)
                     /locus_tag="ONT65_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR2329005.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6094..6795)
                     /gene="nagB"
                     /locus_tag="ONT65_001364"
     CDS             complement(6094..6795)
                     /gene="nagB"
                     /locus_tag="ONT65_001364"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR2329006.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7029..8561
                     /locus_tag="ONT65_001365"
     CDS             7029..8561
                     /locus_tag="ONT65_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2329007.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8752..9954)
                     /locus_tag="ONT65_001366"
     CDS             complement(8752..9954)
                     /locus_tag="ONT65_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329008.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10036..10725)
                     /locus_tag="ONT65_001367"
     CDS             complement(10036..10725)
                     /locus_tag="ONT65_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2329009.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10831..11199)
                     /gene="rplL"
                     /locus_tag="ONT65_001368"
     CDS             complement(10831..11199)
                     /gene="rplL"
                     /locus_tag="ONT65_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR2329010.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11252..11755)
                     /locus_tag="ONT65_001369"
     CDS             complement(11252..11755)
                     /locus_tag="ONT65_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR2329011.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11959..12086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12165..12854)
                     /gene="rplA"
                     /locus_tag="ONT65_001370"
     CDS             complement(12165..12854)
                     /gene="rplA"
                     /locus_tag="ONT65_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR2329012.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12958..13380)
                     /gene="rplK"
                     /locus_tag="ONT65_001371"
     CDS             complement(12958..13380)
                     /gene="rplK"
                     /locus_tag="ONT65_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR2329013.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            complement(13767..14006)
                     /locus_tag="ONT65_001372"
     CDS             complement(13767..14006)
                     /locus_tag="ONT65_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329014.1"
                     /translation="MKKEYGYPTLKVICKASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKITMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(14011..14430)
                     /locus_tag="ONT65_001373"
     CDS             complement(14011..14430)
                     /locus_tag="ONT65_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2329015.1"
                     /translation="MNTSSFEHIVRIQFNALMITVIKCTVKSRNRQFARRSKREVLFY
                     ELSDTKNLECVTEDKYSCDYISFEVLNFTIQISNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTLLKERN"
     gene            complement(14671..15075)
                     /locus_tag="ONT65_001374"
     CDS             complement(14671..15075)
                     /locus_tag="ONT65_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HCR2329016.1"
                     /translation="MKKPKVAFVCVHNSCRSQIAEALGKYLSSNVFDSYSAGTETKPQ
                     INQDAVRLMKEMYQIDMEKTQYSKLLSEIPQVDIVITMGCNVNCPTIPCKYREDWGLN
                     DPTGKNDDEFLKTIHSIHAKILELTEKIKIMR"
     gene            complement(15105..15437)
                     /locus_tag="ONT65_001375"
     CDS             complement(15105..15437)
                     /locus_tag="ONT65_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2329017.1"
                     /translation="MKLFGKKKETKSCCCGGNCTSETMQNAENKKQEQGIKILGSGCA
                     KCMELEKATRTAISELQLDYEIEHITDFAEIASYGVMSTPALVFNGEVISYGKVLTVD
                     EVKALLSK"
     gene            complement(15454..16449)
                     /locus_tag="ONT65_001376"
     CDS             complement(15454..16449)
                     /locus_tag="ONT65_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2329018.1"
                     /translation="MWLFFQDQILGMKWLNGLIGNGLSLLGLDINSRLGGSIQFFLYD
                     VIKITILLCLLIFFISYIQSYFPPERSKKILGRFHGIGANIISALLGTVTPFCSCSSI
                     PLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGAKVAFAYVIVGLIIAVIGG
                     TLIEKMHMEKYVEDFVKNASNVDISSPTLTKKDRVQYAKEQVVGTFKKVFPYILIGVG
                     IGAVIHNWIPETWIESILGSNNPFGVILATLVGVPMYADIFGTIPVAEALLSKGAQLG
                     TILSFMMAVTTLSLPSLIMLKKAVKPKLLTLFIAICTFGIIIVGYLFNIFSTLFI"
     gene            complement(16545..16850)
                     /locus_tag="ONT65_001377"
     CDS             complement(16545..16850)
                     /locus_tag="ONT65_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2329019.1"
                     /translation="MNDFSKDAKIFKALCDTKRLTILDYLKSGEKCACVLIENMNIGQ
                     SALSYHMKILCDSGIVNARQEGKWTHYSLSKSGSEYASKRLLELTTPNAENQSICCK"
     gene            complement(17078..17713)
                     /locus_tag="ONT65_001378"
     CDS             complement(17078..17713)
                     /locus_tag="ONT65_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2329020.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRTEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            18223..19365
                     /locus_tag="ONT65_001379"
     CDS             18223..19365
                     /locus_tag="ONT65_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2329021.1"
                     /translation="MNNLEFALEMKNKRLASGLSQRELAGLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRLPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGTGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGAECRCKNYENVQSGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            19358..19564
                     /locus_tag="ONT65_001380"
     CDS             19358..19564
                     /locus_tag="ONT65_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2329022.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            19643..20836
                     /locus_tag="ONT65_001381"
     CDS             19643..20836
                     /locus_tag="ONT65_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2329023.1"
                     /translation="MREKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKRKKPSLRELEQQIRRDIEDGIDSAGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDMLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKNKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGRPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASRNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            21152..21820
                     /gene="sdaAB"
                     /locus_tag="ONT65_001382"
     CDS             21152..21820
                     /gene="sdaAB"
                     /locus_tag="ONT65_001382"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR2329024.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21833..22705
                     /gene="sdaAA"
                     /locus_tag="ONT65_001383"
     CDS             21833..22705
                     /gene="sdaAA"
                     /locus_tag="ONT65_001383"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR2329025.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22796..23815)
                     /locus_tag="ONT65_001384"
     CDS             complement(22796..23815)
                     /locus_tag="ONT65_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2329026.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23988..24893)
                     /locus_tag="ONT65_001385"
     CDS             complement(23988..24893)
                     /locus_tag="ONT65_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2329027.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24952..25497)
                     /gene="nusG"
                     /locus_tag="ONT65_001386"
     CDS             complement(24952..25497)
                     /gene="nusG"
                     /locus_tag="ONT65_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR2329028.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25598..25768)
                     /gene="secE"
                     /locus_tag="ONT65_001387"
     CDS             complement(25598..25768)
                     /gene="secE"
                     /locus_tag="ONT65_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR2329029.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25792..25944)
                     /gene="rpmG"
                     /locus_tag="ONT65_001388"
     CDS             complement(25792..25944)
                     /gene="rpmG"
                     /locus_tag="ONT65_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2329030.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26106..26750
                     /locus_tag="ONT65_001389"
     CDS             26106..26750
                     /locus_tag="ONT65_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2329031.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26806..27309)
                     /locus_tag="ONT65_001390"
     CDS             complement(26806..27309)
                     /locus_tag="ONT65_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2329032.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27322..27368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27906..28871
                     /locus_tag="ONT65_001391"
     CDS             27906..28871
                     /locus_tag="ONT65_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2329033.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28970..30142)
                     /locus_tag="ONT65_001392"
     CDS             complement(28970..30142)
                     /locus_tag="ONT65_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329034.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30129..>30254)
                     /locus_tag="ONT65_001393"
     CDS             complement(30129..>30254)
                     /locus_tag="ONT65_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329035.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9576 a         6012 c         5379 g         9287 t
ORIGIN      
        1 ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata
       61 attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag
      121 aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa
      181 gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc
      241 gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc
      301 ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa
      361 tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag
      421 tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt
      481 caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa
      541 tgtcaaacca aatacgatga cttttctatc tattatggaa aaaatcaatt acttgatcga
      601 tcaagaatat atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct
      661 atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt
      721 ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga
      781 tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct
      841 tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg
      901 acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa
      961 ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg
     1021 gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc
     1081 aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt
     1141 aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc
     1201 tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct
     1261 tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt
     1321 atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac
     1381 agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg
     1441 tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa
     1501 tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg
     1561 ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt
     1621 attaggaacg gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag
     1681 ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc
     1741 atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg
     1801 attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga
     1861 ggattcatta gactttacaa atatttactt catgctgttg aattattgga cacttgttga
     1921 aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta
     1981 tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa
     2041 agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa
     2101 acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg
     2161 ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga
     2221 agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga
     2281 gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta
     2341 tgcaatggca caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga
     2401 aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa
     2461 tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt
     2521 tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca
     2581 tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt
     2641 atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc
     2701 tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc
     2761 gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc
     2821 tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc
     2881 atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt
     2941 cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa
     3001 atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta
     3061 caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt
     3121 tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca
     3181 aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag
     3241 gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc
     3301 tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct
     3361 ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag
     3421 ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga
     3481 tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag
     3541 gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt
     3601 taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca
     3661 ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt
     3721 ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta atattatcgt
     3781 cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc
     3841 tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa
     3901 caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata
     3961 caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa
     4021 agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata
     4081 aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa
     4141 gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa
     4201 ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc
     4261 cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat
     4321 aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt
     4381 actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc
     4441 ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg
     4501 ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac
     4561 taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt
     4621 ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg
     4681 taaaaaaact gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat
     4741 aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg
     4801 tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt
     4861 aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata
     4921 ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat
     4981 actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc
     5041 agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg
     5101 acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg
     5161 atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga
     5221 tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc
     5281 acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct
     5341 tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc
     5401 actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc
     5461 atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc
     5521 gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc
     5581 acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct
     5641 aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct
     5701 cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca
     5761 aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt
     5821 gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag
     5881 cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt
     5941 catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa
     6001 acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct
     6061 gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc
     6121 gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac
     6181 aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac
     6241 gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc
     6301 ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac
     6361 tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc
     6421 gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca
     6481 tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt
     6541 atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc
     6601 aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat
     6661 ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt
     6721 gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac
     6781 acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc
     6841 tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca
     6901 ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat
     6961 gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag
     7021 gagttattat gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc
     7081 tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa
     7141 acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg
     7201 gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg
     7261 atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg
     7321 aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg
     7381 aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct
     7441 tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga
     7501 aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc
     7561 cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc
     7621 tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat
     7681 ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata
     7741 ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc
     7801 aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag
     7861 ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc
     7921 tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca
     7981 cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac
     8041 ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg
     8101 gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc
     8161 acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat
     8221 tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac
     8281 gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat
     8341 taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt
     8401 tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag
     8461 acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc
     8521 aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg
     8581 aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc
     8641 aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt
     8701 tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt
     8761 attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg
     8821 aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc
     8881 tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag tattatttcg
     8941 gttgcttgct gcttcaccta gcccttgtac tcgtgtttga ttggcgactt gaccaaattg
     9001 attcccgacg tctaataaaa taatagctaa aagcaaaatg gccaaatggc ttccgccaac
     9061 caataaagca ataaagctta ttgtttgcat aacaagtccc atatacacga tttttctttc
     9121 agaataagaa tctgccagtc gtcccacgat aggtgcagca actgctccag acaaaccaaa
     9181 aatcgctaat atccctgctt cgaatgttcc ccaattgtag gcgggactgc tgatataaaa
     9241 aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc
     9301 ttctcttaat agacgttgag aagcaactaa atgcggcaaa cttttaagtg aatcaaggta
     9361 tgaaacagct gtactacttt gctggacaga acgcggcatt ttccagtaaa gcagaagtgt
     9421 aagaattccg actgcacata aagcaaataa atagatcatc cgccaagaaa aaagactggc
     9481 aacaatacca gataccgtcc gagaaagcaa aatccccacg agtaaaccgc taagcaatgt
     9541 acccatgacc ttccctcttg cttttggtcc cgctagtacc gcaccgtaag gaatgatgat
     9601 ttgcggcacg atcgaaagta atcctattaa aaaagaagcg catgcaaata acgtaaacga
     9661 aggcgcaaaa aaagcggcca acagagagac ggcagataac gctgccattc gaatgatcaa
     9721 ttttggacga tccaccacgt cccctaaagg aactaaaaac agtaacccta acgcatagcc
     9781 taattgggtc aacatcgtca atatcccgac tgcacttgtg ctaacagata aactagctgc
     9841 tacttttgtt ccaatcggct ggatgtaata catattggca acaacaacgc cacaagtgat
     9901 tgataatagt aaaataagcg aagttgttaa aggttttttt tcattactct tcattgattt
     9961 cccccatttc ctatcttagt ttacaactta tggaaaagat tggaaataga aaaatacaac
    10021 tattcgccac acttattatt tgtgcttttg ttttccttgt tttttcttgc gatctttata
    10081 aatgaagaca actaacccta ctagcacagt aacacttgca accaacgtga ctgcagtttt
    10141 gttatatccg tgaaacagag cttctccacc acacgcatac aacacagacg aagggatgac
    10201 tccaatcacg atcgacaata tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa
    10261 attgaccata gaagatggaa taaccggtat catatagcct aacattacac ctatttttgg
    10321 atgtttcatt tgtcggattg cttgcaccca atgattcgtt tcggttttct tatctaaaaa
    10381 ttttaagcga tgcataagga aaattgcagt gatgttacca agagcattac ccaaaatgtt
    10441 gatgatcgca cctgtcagtg gtccaaagct caaaccagca atgactccaa ctactgaagt
    10501 aggaatacct ggaacagcac acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa
    10561 agtcccatgc gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa
    10621 taattggatg tctgtctgat attctaaata caaccgatag cccactacca gtatcagaat
    10681 gattcccaac gagatcaata tcaatcggat tattttcttt ttcatatttc ctcctgaaaa
    10741 aaatctatat tcattaaata atagtttaac ataaaaacaa aaaagcaccg aaatccaaca
    10801 taggatctca gtgcttttaa atagtaaaaa ttatttaaca gttacagatg cgccaacttc
    10861 ttcaagttta gctttcaatt cttcagcttc ttctttagat acgccttctt taactggtgc
    10921 aggagcgcca tcaactacag ctttagcttc tttcaagcct aaaccagttg cttcacgaac
    10981 tgctttgata actttaactt tttggtctcc agctgcagtt aattctacag tgaattctgt
    11041 ttgttcttca gcagcagcag cgccaccagc agcagctaca gctacaggag cagcagcaga
    11101 tacgccaaat tcttcttcaa tagctttaac taggtcgtta agttctagga tagttgcttc
    11161 ttttaactca gcaacgatgt tttcaatgtt caatgccatt ataatttcct ccgtttaatt
    11221 gttctttttt atttaatgct ttatgcggca attaagctgc atctccatct tctttgtctg
    11281 ccactgcttt gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca gatagcaaca
    11341 tagatagaag tccttcgcga tttggcaatt ttgccaatgc tgtgatttct tccaatgaag
    11401 agactttacc ttcgatgata ccgcctttga tttctaatgc ttttgcttct ttagcaaatt
    11461 cgtccatgat tttcgctggt gcgactacat cttcgttact gaatgcaaca gctgtaggtc
    11521 cagtgaatac ttcgtccatg ccttcaaggc cagctttttt agctgcacgt gaaagaatag
    11581 aatttttgat aactttcatt tctacgttag catcacgaag ttgtttacgt aaacgtgtta
    11641 cttcatcaac tgttaatccg cggtagtcaa cgactactac tgatgcagct gctgtaaatt
    11701 tttcgcttac ttcatcaacg attgctgctt tttttgcgat aattgcttca ctcatttttg
    11761 agtttcacct cctgaatttt gatggaagag ttttttgcac tgaaaaggca agtcactttt
    11821 atagttactt tgctcaacca gtattattca tcacctattc acttcgttca tagtgattct
    11881 actcgctttg ctcgaaagga aactcctttc gctttgctca accagtattg ttcatcatct
    11941 cttcacttcg ttcatagtga ttcacaataa aaaactctat gccaccgcag acatagaggg
    12001 actattgaat atattcaata aacgtcctcg gtaggaaatt aaggcttgat gccacctact
    12061 gtcttcggta caagttaatt attaacctca actagcttag catgctaaac tagttgagtc
    12121 aagaattatt tttcagttgg atcaatcaac attctaatat ttaattagaa agaagcttgg
    12181 tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta catttttcat gtattgacct
    12241 ttagctgctg atggtttagc ttttagcaat acatcatgga ttgctgcaaa gttttcaact
    12301 aatttttcat cgtcaaatga aacttttccg attggtacat ggatatttcc agctttgtcc
    12361 acacggtaag ttactttacc agctttcact tcgtttactg cttttgttac gtccattgta
    12421 actgtaccag ttttagggtt tggcattaag ccttttggtc ctaatactcg tcctagacga
    12481 cctacagtag ccatcatatc aggtgttgca acaactacat caaagtcaaa ccatccgttt
    12541 tggatttttt gaaccatgtc gtcgtcacct acgaaatctg caccagctgc ttcagcttct
    12601 tttgctttgt ctcctttagc aaaaactaaa acagtttgtg ttttaccagt tccgtttggt
    12661 aatacaacag caccacggat ttgttggtct gcttttttag ggtctacgtt caaacggtat
    12721 gcaacttcaa ctgttgcatc aaattttgca atgtttgttt cttttgctaa agctactgct
    12781 tcggcaacag gataaacttt acttgaatca acttttttta atgcatcttg catttttttg
    12841 ctctttttag ccattttggt tcctccttga ttgtggttat aacggttgta cctcccacgt
    12901 gttctatatc tttcaatata gagccaactg agaagctgct gtcttagggt gtatagatta
    12961 ttctacagtg atccccatgc ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac
    13021 gttagctgcg tttaggtctt ccatttttaa ttcagcgatt tctcttactt gatcgctaga
    13081 aactttcgca actttatttt tgtttggttc gcctgaacct ttttcgattt tagctgcttt
    13141 tttcaataga acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta
    13201 tacagaaata acaaccggaa tgatcaaacc tgcttgatca gctgtacgag cgttgaattc
    13261 ttttgtgaat cccatgatat tgatacccgc ttgacctagt gcaggaccta ctggtggagc
    13321 tggagttgct ttacctgcag gaatttgcaa tttaacgatt ttttctactt tctttgccac
    13381 gagacatacc tccttaagtc cgtgatgtgg ttaattggag atgcatattt ctcctcccac
    13441 gttttatctg atctgaaaaa atccagacaa ataatgtgca aaacaatgca cactggtaca
    13501 gtatagcaaa tttatttgtt ttttcaagat ttttttctgc tatttttaca ttttgcttga
    13561 ataaacgcta aatttaatat aaattttata tctcattttc aattatccgc ataaatacta
    13621 tacattcaag tattttaagg tatagaaaaa cactcatttt accacgaatt taccacgatt
    13681 gaatgagcct tgatatgaca caaaaaacaa catgggagat agcacaaata tctgtgtatc
    13741 tcccgttttt cattcatgta atatgattac tttactctga tttcaaattt caagattgct
    13801 ttcatcattt ctgtatgaat ttgacctttt aattcttcat caacctgcat agtaatttta
    13861 ccactttcag agtggtaaaa cggacgcaag cataatttac aaatataagc gtcataaaac
    13921 ttcaaaatct ctcttatggc agtttcattt ccagcagacg ctttacatat tacttttaat
    13981 gtagggtagc catattcttt tttcattgtt tcaattcctt tctttcaata atgtttttaa
    14041 tttcttcaat ccgttacttc ttctgcgata aatagcagaa cgtgatacat gatataattc
    14101 tgcaatttct tggtcgctca tgctttgaaa atagcgtaat agaattacat cacgctcttt
    14161 actcgataac ttatataaag caacagcaag tttctcgttt gatatttgaa ttgtgaaatt
    14221 caagacttca aaagaaatat aatcacaaga atatttatcc tcagttacac attcaagatt
    14281 tttcgtatct gataattcat agaataaaac ctcacgcttg gaacgtcttg caaactgtct
    14341 atttctgctt tttactgtgc atttaataac agttatcatc aaagcattaa actgtattct
    14401 gacgatatgc tcgaaagaag atgtgttcat aatctcaccc cctttctgca tacgccgaaa
    14461 tatataaacg ggaataccgc ctataccttt tggtaatgta aaaaagaaag caagaaaaaa
    14521 gcccgcacaa aacatatagt ctgcacgagc cttcaaaatt tccttatatt cagatgttga
    14581 taattgccat attagactgt ttaatagtgt gcttaaaaca atttatgaca atattcttcg
    14641 ttaatatttt catttagttt ctccttgaaa tcaacgcatt atttttattt tttctgtcaa
    14701 ttcaagtatt tttgcatgga ttgaatggat tgtctttaaa aattcatcat catttttccc
    14761 tgtagggtca ttcaaccccc aatcctctct atatttgcat ggtattgtag gacaattcac
    14821 attgcacccc attgtaatca caatatctac ctgtggtatt tcagataaca atttagaata
    14881 ttgtgttttc tccatatcta tctgatacat ttctttcatt agacgaactg catcttgatt
    14941 gatttgtggc tttgtttctg tgcctgcgga ataactatca aaaacatttg aagaaagata
    15001 cttgcccaat gcctccgcaa tttgactgcg acaagaatta tgaacacaga caaaggctac
    15061 tttaggtttt ttcataaatg aattaccaac ctttctttgt tttattattt gctcaataat
    15121 gcttttactt cgtctacggt caatactttt ccataggata ttacttcgcc attaaacacc
    15181 aatgcaggtg tagacattac tccataacta gcgatttctg caaaatctgt aatgtgttca
    15241 atctcataat ctaattgaag ttccgatatt gctgttctag ttgctttttc caattccata
    15301 cattttgcac agcccgaacc taatatttta atgccttgct cttgtttctt gttttctgca
    15361 ttttgcattg tttctgatgt gcaatttcca ccacaacaac atgattttgt ttcttttttc
    15421 tttccaaata atttcatttc ttattcgctc cttttaaata aataatgtac taaaaatatt
    15481 aaagagataa cctacaataa taatgccaaa cgtacaaatg gcaataaaaa gggtcaatag
    15541 tttaggcttt actgcctttt tcagcattat taaagacggc aaactcaatg ttgttacagc
    15601 catcataaat gataaaattg tacctaattg tgcacccttt gaaagtaatg cttctgctac
    15661 tggaattgtt ccaaaaatat cagcatacat aggaactccg acaagagtgg ctaaaataac
    15721 accaaacggg ttattactgc ccaagatact ttcaatccat gtttcaggta tccagttatg
    15781 gataactgct ccgataccca caccaatcaa aatgtatgga aatactttct tgaatgttcc
    15841 tacaacttgt tcttttgcat attgaactct atcctttttg gttaaagtag gagaactaat
    15901 atcgacatta cttgcatttt tcacgaaatc ttccacatat ttttccatgt gcattttttc
    15961 aattaaagta ccgccaatta cagcgattat caagcctaca atcacataag caaaagcaac
    16021 tttagccccg aaaatactca ttagcaatac aagactccct aagtcaacca taggcgaaga
    16081 aatcaaaaag gaaaaagtta ctcctaacgg taatcctgca cttgtaaaac ctataaacaa
    16141 cgggatagaa gaacacgagc aaaatggtgt aactgttcct aacaatgcgg atatgatatt
    16201 tgcaccgata ccatgaaatc gccctaaaat ttttttactt cgttcgggtg gaaaataact
    16261 ttgaatatac gatataaaga aaatcagtaa acataacaag attgtgattt tgattacatc
    16321 gtataaaaag aattgaatac ttccacctaa acgactgtta atgtctagcc ctagcaatga
    16381 tagcccatta ccaattaagc cattcaacca tttcatacct aatatttggt cttgaaaaaa
    16441 tagccatatt gaatttacta cttccaataa agtccctcct ttacatgaca tatcaatttt
    16501 ttttgatgtg ataaagtttt ataaaagcca cctttcgatg acctttattt gcaacaaatt
    16561 gattgatttt cagcatttgg tgttgttagt tctaacaatc gttttgacgc atattcactt
    16621 ccacttttac ttaggctgta atgagtccat tttccctctt gtcttgcatt aacaatacca
    16681 gagtcgcaaa gtattttcat gtggtaggat aaagccgatt gtcctatgtt catgttttca
    16741 atcagaacac acgcacattt ttctccgctc tttaagtaat ctaaaatcgt aagtcttttt
    16801 gtatcacaaa gagccttaaa aatttttgcg tctttagaaa aatcattcat tattatcact
    16861 cctcacatca aataattttg atatatttat cataaggctt acaacatgat ttgtcaacac
    16921 attacatcaa aatattttga tgtattattc ttttttcctc ttgattaccc ataacagaaa
    16981 agaaaaagcc ctgtcaagag cttgccacca ttggtggtgc tttgcactct ttactgggct
    17041 ttttgtttgc tgtatcttcc atgcaagagg aaaaagatta ttcgtcctca tcatcaaaat
    17101 ggttattcct taatacttcc cgtaataatt ccatatcttc ttttgaatta gggtttatca
    17161 ttttgaccac ttgataaggt gtcctatatt tatgtccctc tatgctttcc ccaaacatat
    17221 ccatgctgta taaatcatca tagcggtcta acagtttttg aaacttcaaa tgctggataa
    17281 attctttgat tgggtaaagg tcggacgctt caatgtttgt tccattgatg tagtcggtaa
    17341 tatattctcg taacttttct aattcatatt ccagccattc ttcctcgctg tcaaagaagt
    17401 tgtcataatg tccatgtttt aattctgcat ttagacgttc ttctgttctt aaaaactgac
    17461 agacttcttt ttcggactga ttgacatatt tccattcccc cgacaacaat tcattcggcg
    17521 taacgtccag cacttccatt atcttttcca gcgtttcata agcaggatag ttcaaccctc
    17581 tttcaatctt ggaaaggctc tgcatattga taccaatttt gtccgcaagt tcctgttgtt
    17641 tcattcccct aaattttctt atggtctgta tattatttcc taaatggctt attttcattg
    17701 cataaccctc cataactcgt tatatcttcg ctgctataag tctatcatgc ttaatatgat
    17761 ttgtaaagat aaaaaaagat attgacaaat aagcatataa gaattactat gttcttgtaa
    17821 tcaaataagc acaatagaat gaaatacaaa aacattcaaa agaaaatatc tatctgatta
    17881 aacccgtaga tatgaatatc ggttgaaatg gaacttgttt gaaaggacaa acacaggact
    17941 tgccgacaga gcattgtagg actgttggcg tgcgtcagca acaacgccat gacggcttgc
    18001 cgtcaaagga cggaaatgct ttgtcggctg gcgaacttgt gagccttgtc tgtgcccccg
    18061 ttatctaaca ggggggcact atttcacaaa agacaagtca aaaaccatac aaccccaaag
    18121 ctgtaaggag taatgaggag ttttaagaag atagtaactt cacattcttt tgaatagtgg
    18181 tttacaggta tttcaaggct gttgtgggat tggaggaata caatgaacaa tttagaattt
    18241 gcacttgaaa tgaaaaacaa acgtcttgca agcggtcttt cacaaagaga acttgcaggt
    18301 cttacccatg tatcaagata tagtatcaac cgctttgaga acggaaaagc caacgccagc
    18361 aaggaaacac aaaatattat cctgcgttgt ctgaattact atatctgcga taagcctttt
    18421 tatctgctcg ttgattatct gtctgtccgt ttaccaacga ctgacgcatt agaagttatc
    18481 cgaaaggtgc ttggcatgaa agcagattat tttatccatt atgactatgg gtattatggc
    18541 tataaggagc attacgccta cggggaaatc aaggtcatgg cttctgatga tgaacacatg
    18601 ggcgtatttt tggaattaaa agggacaggc tcacgcaaca tggaatatgt cttacaggca
    18661 cagaacaggg attggtattc atttttaaac cgctgtcttg actgtggcgg tgttatccga
    18721 cgttttgatt tggcaatcaa tgatatgtgc ggtctgctgg atattcccgt tttgtctgaa
    18781 aaatataaaa atggcggtgc ggaatgtcgc tgtaaaaact atgaaaatgt acaaagtgga
    18841 aaattaagtg ggaaaaaccg caatttagca agtacccttt atatcggttc aaaggcaagc
    18901 acaaaatatt tctgcctgta tgaaaaacaa aaggaacagg caacgaaaaa gaaacatacc
    18961 gatattatca accgttttga aattcgtctg cgtgataaaa aggcagtaca ggcagttgag
    19021 gaattgttat tgacatataa ccctcatggg ctggtgtttt acctcattac tgattttgtg
    19081 caatttcccg actatccgtt gtgggagata ttcatttccc atgacagttt accttttgaa
    19141 atgaaccccg tacctgtcaa tatggaacgc actctgcaat ggctggaaag acaggtcatg
    19201 ccgtcaatcg tgatgataga ggaaatcgac aggctgacag gctcaaacta catgaaaatg
    19261 attgatgaat gtacccacct ttccgaaaaa caggaaatgc ttgtagaaca gatgtgtacg
    19321 gatatagcag atgtaatcga aagtgagggg gtgttttatg agtaatgcac aggatattcc
    19381 cgtatgggaa aaatataccc ttaccattga agaagcgtca aagtatttcc gtatcggaga
    19441 aaacaagtta agacgattgg cagaggaaaa caaggacgct ggctggctca ttatgaatgg
    19501 caaccgcata cagattaaac gccgacagtt tgaacaggtt attgataaat tggacgcaat
    19561 ctaatgcaaa tgagccttgt atgtgttatg ataagcacaa gtcatatcaa ggctctttcc
    19621 aacaaggaaa ggagcagaca ccatgagaga aaaaagacgg gataacaaag gtcgtatcct
    19681 gcatactgga gagagccaac gaacagacgg aaaatactta tataaatatg tagacgcatt
    19741 tggaaacaca aaatatgtgt atgcttggag attgacaccc acagacccga caccaaaggg
    19801 aaaacggaaa aaaccctcac ttcgagaact ggaacagcag ataagacggg atattgagga
    19861 cggtatcgac agcgcaggca agaaaatgac gctttgccaa ctctatgcca aacagaacgc
    19921 acaaagggca aatgtgaaga aaagcacaca aaaacaacgg gaacaactca tgcggttatt
    19981 gaaagaggac aaattaggtg ctaggagcat tgatacaatc aagccctctg acgctaaaga
    20041 atgggcgtta cgcatgaaag acaagggctt ttcctacaat accattaaca accataaacg
    20101 ctcgttaaaa gcgtcattct atatcgccat acaagacgat tgcgtaagga aaaatccctt
    20161 tgatttcaaa ttaagtgaag tcctagaaaa tgataccaaa gagaaagtcg cattgacaga
    20221 ggaacaggaa caagccttac tctcattcat caagacggac aatgtgtatc acaaatatta
    20281 tgatgatatg ctgatattgt taaaaacagg acttcgtatc tcggaactgt gcggactgac
    20341 agtagccgat attgatttca agaatgaagt tgtgattatc gaccaccagt tattaaagaa
    20401 caaggaacag ggctattata ttgaaacgcc taaaacaaag agcggaataa ggcaagtgcc
    20461 attaagcaga gaaacgatac aggcatttca acgggttatg aaaaaacgcc caaaggcaga
    20521 accatttgtg atagacggac ggagcaattt tctgtttgtc aatcataagg gtaggcccaa
    20581 agttgcgatt gattacaacg ccttatttgt ccgaatggta aagaaataca acaagcacca
    20641 caaggacaat cccttgccac atatcacacc gcatacgcta cgccatacat tctgcacaag
    20701 gctggcaagc aggaacatga acccgaaaga tttacagtat atcatgggac attcaaacat
    20761 cagtatcaca atgaactggt atgctcatgc gtccatagat accgcaaaat cagaggttca
    20821 gcgtctaatc gcataagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    20881 aagaagatag atctagatat gtgaggttta ccacaaaaac aaaatgcccg tagagcctat
    20941 aaaataaggc tttgcggaca tttaagaaga tataaaaaga tagtcaaaaa gacatatata
    21001 atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt tacacttgcg
    21061 ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc cttgtacaaa
    21121 gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata gaagtgtctt
    21181 tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg gagctgctag
    21241 aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag atatttatct
    21301 atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat tagttggcgg
    21361 cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat tggcatatga
    21421 agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt
    21481 aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt cgatcggcgg
    21541 tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca tgggtactcc
    21601 taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga ctagtatact
    21661 ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa
    21721 tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa
    21781 acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt
    21841 atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag aattgatgat
    21901 tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa
    21961 aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac
    22021 aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg cagacttttt
    22081 aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg aagtcaatgc
    22141 aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt
    22201 gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg aattcttatt
    22261 taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg
    22321 gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt tggtttgtgc
    22381 aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga aaaatatgat
    22441 gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc
    22501 tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta tcgaaagtgt
    22561 cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat
    22621 tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac gacttgcgga
    22681 agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta agagattagt
    22741 tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt ttttattaat
    22801 ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga
    22861 tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc ccacctagat
    22921 tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt gccagagctt
    22981 tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca cgtttgttga
    23041 ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct agttgacgtc
    23101 caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt actgcatctt
    23161 ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg attgttttga
    23221 tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt ccttttcctt
    23281 cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt ggtaatggaa
    23341 attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta
    23401 aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca ttgaatctgt
    23461 cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt
    23521 ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca gcagctactc
    23581 ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct gatggcgctt
    23641 ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg gctgagtcga
    23701 ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta ttttctttca
    23761 tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct tccatgcgct
    23821 cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt tcttcattac
    23881 ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat aaatcatact
    23941 aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta ttctttccaa
    24001 ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact atgagaggca
    24061 ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa caacgcatag
    24121 accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc atctagttga
    24181 ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg ctggtaatct
    24241 tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag agactgcgca
    24301 ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc
    24361 aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc tcccatggaa
    24421 tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa aagacaagtt
    24481 ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa tacttggacc
    24541 atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc atctgttcct
    24601 actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg caacatccct
    24661 ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat agtggttttt
    24721 tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt agtatttatc
    24781 ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc tgctgcagtt
    24841 gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc catatagaaa
    24901 aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt attaatcgat
    24961 tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca tatcgatgtt
    25021 gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta atccagcaaa
    25081 tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat tttgtcgcag
    25141 gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg gtgctggttt
    25201 gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc ggacaacata
    25261 ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg ttttatgaac
    25321 gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa caacgacacg
    25381 gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg cttttacttt
    25441 gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag attccattga
    25501 aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt tttctttcgc
    25561 ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc cagccaaata
    25621 agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata gaagtttcga
    25681 tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact tgtttgattt
    25741 catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg tttatttcgt
    25801 ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt ccaaacgttc
    25861 gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa cggaacaagc
    25921 gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc agacttatac
    25981 ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact atctattgtt
    26041 ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca attggaggta
    26101 tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc gttacagaga
    26161 ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt tgtgtgccga
    26221 tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat atctatcgtc
    26281 ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa aacgctacaa
    26341 agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa gagcttcctt
    26401 atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat agtacgttgc
    26461 ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg acaattgctt
    26521 ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa cacagcagca
    26581 ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt acattgatca
    26641 cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa
    26701 agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg
    26761 aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga cacgcttttc
    26821 aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca tgacgataaa
    26881 ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg ttggaactaa
    26941 catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata ctaaacgcat
    27001 ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg
    27061 gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat agacgatgac
    27121 cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat tcaaactttc
    27181 tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag ttaacgctag
    27241 ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc tgcttttcgt
    27301 tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg ttgatgaacc
    27361 gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca atccgtctta
    27421 tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata
    27481 tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg aagtaggatc
    27541 tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg aatttgagca
    27601 acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca tgccgaacct
    27661 tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt tgtgtttttc
    27721 ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga attaattttg
    27781 taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa agcttgctaa
    27841 ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa tagcaggagg
    27901 aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc ggaaatggct
    27961 ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac tatacacttc
    28021 attatagtga atacaaagga cacgatgctg agattgctga aactttggca aaagaaacat
    28081 tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt ttgatcatcg
    28141 taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg gaagtagaat
    28201 tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg gcaggccttc
    28261 caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca gaaaaagtcc
    28321 acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt aataatttcg
    28381 gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca aaaaagcgat
    28441 tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt gctttattca
    28501 ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt ttttcaaacg
    28561 catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg attgttccaa
    28621 ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc aatttcttta
    28681 aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg aaatatttcc
    28741 atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat ggtcaagaag
    28801 atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact aaacagttgt
    28861 tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt ttattcggaa
    28921 aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt taattctttg
    28981 cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg atcattgaaa
    29041 acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct gcaaatgttt
    29101 gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa tatgttggtg
    29161 tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca aacaccccac
    29221 cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt tctgagtttt
    29281 ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac aagaaagtat
    29341 ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac agaaccatta
    29401 gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag gttactttgc
    29461 gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc tctttgcgat
    29521 taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac ccataagaca
    29581 agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat cccgcactta
    29641 gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct gcatcgattg
    29701 ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact tgctgtgtaa
    29761 caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc atctttccta
    29821 cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca cgattaaaat
    29881 aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact gtttccttgt
    29941 cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta atcaaggcga
    30001 ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa aagaatgtgg
    30061 caacaacagt gataattaca cctatcagta aattcgattt atttgcttta agcaatgtcc
    30121 aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt tcctaacgca
    30181 aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc caaatcagtg
    30241 tagaaataaa gttt
//
LOCUS       DAJSBC010000029        28197 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSBC010000029 DAJSBC010000000
VERSION     DAJSBC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.029"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            160..1302
                     /locus_tag="ONT65_001394"
     CDS             160..1302
                     /locus_tag="ONT65_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2329036.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            1318..2529
                     /gene="thiI"
                     /locus_tag="ONT65_001395"
     CDS             1318..2529
                     /gene="thiI"
                     /locus_tag="ONT65_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR2329037.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            3102..3749
                     /locus_tag="ONT65_001396"
     CDS             3102..3749
                     /locus_tag="ONT65_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2329038.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    3878..4094
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4142..6787
                     /locus_tag="ONT65_001397"
     CDS             4142..6787
                     /locus_tag="ONT65_001397"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR2329039.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            7097..8410
                     /locus_tag="ONT65_001398"
     CDS             7097..8410
                     /locus_tag="ONT65_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR2329040.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            8400..9053
                     /locus_tag="ONT65_001399"
     CDS             8400..9053
                     /locus_tag="ONT65_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2329041.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            9108..9659
                     /gene="radC"
                     /locus_tag="ONT65_001400"
                     /pseudo
     CDS             9108..9659
                     /gene="radC"
                     /locus_tag="ONT65_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            9790..10875
                     /locus_tag="ONT65_001401"
     CDS             9790..10875
                     /locus_tag="ONT65_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2329042.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            10872..12764
                     /locus_tag="ONT65_001402"
     CDS             10872..12764
                     /locus_tag="ONT65_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2329043.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            12771..13148
                     /locus_tag="ONT65_001403"
     CDS             12771..13148
                     /locus_tag="ONT65_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2329044.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            13302..14084
                     /locus_tag="ONT65_001404"
     CDS             13302..14084
                     /locus_tag="ONT65_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2329045.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT65_001405"
     CDS             complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT65_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2329046.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14999..15058)
                     /locus_tag="ONT65_001406"
     CDS             complement(14999..15058)
                     /locus_tag="ONT65_001406"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2329047.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            15450..16112
                     /locus_tag="ONT65_001407"
     CDS             15450..16112
                     /locus_tag="ONT65_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2329048.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            16347..16490
                     /locus_tag="ONT65_001408"
                     /pseudo
     CDS             16347..16490
                     /locus_tag="ONT65_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            16640..16840
                     /locus_tag="ONT65_001409"
     CDS             16640..16840
                     /locus_tag="ONT65_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2329049.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(17094..18773)
                     /locus_tag="ONT65_001410"
     CDS             complement(17094..18773)
                     /locus_tag="ONT65_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2329050.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(18775..18987)
                     /locus_tag="ONT65_001411"
     CDS             complement(18775..18987)
                     /locus_tag="ONT65_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2329051.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            19380..21110
                     /gene="efrA"
                     /locus_tag="ONT65_001412"
     CDS             19380..21110
                     /gene="efrA"
                     /locus_tag="ONT65_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2329052.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            21107..22867
                     /gene="efrB"
                     /locus_tag="ONT65_001413"
     CDS             21107..22867
                     /gene="efrB"
                     /locus_tag="ONT65_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2329053.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            22962..23159
                     /locus_tag="ONT65_001414"
     CDS             22962..23159
                     /locus_tag="ONT65_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329054.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            23312..23797
                     /locus_tag="ONT65_001415"
     CDS             23312..23797
                     /locus_tag="ONT65_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR2329055.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT65_001416"
     CDS             complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT65_001416"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR2329056.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            24674..25201
                     /locus_tag="ONT65_001417"
     CDS             24674..25201
                     /locus_tag="ONT65_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329057.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            25292..26029
                     /locus_tag="ONT65_001418"
     CDS             25292..26029
                     /locus_tag="ONT65_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2329058.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            26033..26950
                     /gene="miaA"
                     /locus_tag="ONT65_001419"
     CDS             26033..26950
                     /gene="miaA"
                     /locus_tag="ONT65_001419"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR2329059.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            26952..28181
                     /gene="hflX"
                     /locus_tag="ONT65_001420"
     CDS             26952..28181
                     /gene="hflX"
                     /locus_tag="ONT65_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR2329060.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         9420 a         4609 c         5934 g         8234 t
ORIGIN      
        1 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
       61 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
      121 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
      181 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
      241 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
      301 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
      361 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
      421 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
      481 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
      541 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
      601 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
      661 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
      721 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
      781 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
      841 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
      901 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
      961 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
     1021 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
     1081 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
     1141 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
     1201 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
     1261 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
     1321 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
     1381 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
     1441 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
     1501 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
     1561 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
     1621 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
     1681 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
     1741 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
     1801 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
     1861 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
     1921 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
     1981 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
     2041 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
     2101 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
     2161 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
     2221 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
     2281 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
     2341 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
     2401 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
     2461 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
     2521 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
     2581 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
     2641 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
     2701 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
     2761 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
     2821 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
     2881 atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2941 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
     3001 tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat
     3061 gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata
     3121 cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg
     3181 atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt
     3241 ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct
     3301 atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac
     3361 gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa
     3421 gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag
     3481 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
     3541 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
     3601 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
     3661 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
     3721 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
     3781 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
     3841 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
     3901 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
     3961 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
     4021 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
     4081 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
     4141 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
     4201 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
     4261 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
     4321 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
     4381 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
     4441 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
     4501 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
     4561 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
     4621 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
     4681 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
     4741 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
     4801 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
     4861 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
     4921 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
     4981 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
     5041 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
     5101 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
     5161 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
     5221 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
     5281 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
     5341 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
     5401 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
     5461 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
     5521 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
     5581 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
     5641 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
     5701 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
     5761 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
     5821 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
     5881 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
     5941 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
     6001 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
     6061 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
     6121 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
     6181 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
     6241 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
     6301 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
     6361 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
     6421 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
     6481 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
     6541 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
     6601 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
     6661 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
     6721 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
     6781 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
     6841 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
     6901 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
     6961 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
     7021 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
     7081 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
     7141 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
     7201 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
     7261 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
     7321 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
     7381 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
     7441 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
     7501 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
     7561 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
     7621 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
     7681 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
     7741 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
     7801 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
     7861 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
     7921 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
     7981 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
     8041 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
     8101 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
     8161 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
     8221 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
     8281 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
     8341 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
     8401 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
     8461 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
     8521 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
     8581 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
     8641 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
     8701 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
     8761 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
     8821 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
     8881 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
     8941 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
     9001 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
     9061 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
     9121 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
     9181 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
     9241 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
     9301 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
     9361 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
     9421 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
     9481 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
     9541 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
     9601 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa
     9661 ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt
     9721 ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg
     9781 tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta
     9841 ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa atacttagat
     9901 aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa gttgctgtac
     9961 gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt gttagcagac
    10021 ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca gtcaaaaaaa
    10081 gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag ttttcttgat
    10141 tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc aaagggaaga
    10201 aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa tgtcttaaag
    10261 ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa gcaaattatt
    10321 gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga gggtggttta
    10381 agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga caatcaaatc
    10441 catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag gaaggaaaga
    10501 acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat atatgagtat
    10561 agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta ttttgggaaa
    10621 ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag gactggaata
    10681 gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag ggaaggatgg
    10741 gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac gttaaatacc
    10801 tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct cgagagaaag
    10861 gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt gagattagca
    10921 aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat agttgggata
    10981 taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct ttaaggaatc
    11041 gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg aaatattttt
    11101 atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt agaaaaggaa
    11161 cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt acttcaatta
    11221 ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca aaacggggtg
    11281 ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca cctgtaaaag
    11341 ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag aacttttatt
    11401 ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct ttgccagatg
    11461 ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt cggaatacat
    11521 attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat agcttttcct
    11581 atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac atgaaattta
    11641 aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat ttaagtgaac
    11701 taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata ttggaaggac
    11761 tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata ttaatttatt
    11821 ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa ttcgtcctag
    11881 agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg actatgattg
    11941 ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc tgtctattag
    12001 aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa aaggagcata
    12061 tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat aaagtttcag
    12121 aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca ttaaaacaag
    12181 aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg gataaatcag
    12241 gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca agaatgatta
    12301 acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc ccagaaatga
    12361 caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact aaatttcagg
    12421 aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat gaagtcgatg
    12481 atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca aatggttatt
    12541 gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta gattgcactc
    12601 acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag cgtacagaag
    12661 agttattagc catagctaag gataaacaat ggcaaagaca agtagagact aatagccgtg
    12721 ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca atggataaac
    12781 aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa aagacaaggg
    12841 aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt aattttaatt
    12901 caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac gatattaggc
    12961 aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca aaacccaaga
    13021 aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta atggaattag
    13081 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
    13141 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
    13201 gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat tgagtatcaa
    13261 tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat ttgattaacg
    13321 gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta tttggaagtt
    13381 caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc attaacagcg
    13441 atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga aaaaaactaa
    13501 cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt agaccacttg
    13561 aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca aaaagagaat
    13621 ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag gaaccaagct
    13681 atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt tctctatttg
    13741 gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga agagcaatta
    13801 aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat gtaattttaa
    13861 ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa gatgtcgctg
    13921 cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat atagctagaa
    13981 aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag gtgaaagcac
    14041 tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat tttattgcaa
    14101 taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca atttttggga
    14161 agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt tagtgaaaca
    14221 atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta atattagtga
    14281 catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca cgatattcac
    14341 ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt gaaatcaatg
    14401 gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga tgaaaatata
    14461 gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt aaacccaaag
    14521 ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga tagctatatt
    14581 tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta taaggaatat
    14641 taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata tccgtttgaa
    14701 tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat aagcctccat
    14761 caatttctat agcagtaact gatcgactca ttttgactag ctctttggta aaatgtcctt
    14821 ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga ttcaatattt
    14881 cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt ttctggttca
    14941 ttataacctt ctccttactg gttataatga actgacatta agcgttcatt ataaccaatt
    15001 aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa gtacccatac
    15061 ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct actactgcga
    15121 tactggtgca cataattgaa acctccttat gtaactgaat atgattgtat catcttttca
    15181 ttttagaata aacctttata tgtaattttg cacttgacac ttcagatgga cgttttcttc
    15241 aaaataatat gttttgaaac ttgataagag aagccactgt ctttactaaa aagtaaaggc
    15301 tgcgataaag gacagtgact ttaataaagt tgcactgttg tattgtcttt ggataaaatt
    15361 gtctaaaggg aagttttatc cccaacgaaa aaacatctaa agtggtaagt aatgaaagaa
    15421 tgtcgataaa gtaggaatgg gggatctttt tgaatacaat agaatcacag tttgataagg
    15481 ttgcagaaga ttacgatttc gtgaatgagc ttttgaatga ttattcattc tttgtgtcta
    15541 atatgtctcc aaagaaaggc agagcattag atatcggatg tggctcgggt ttgttagtgg
    15601 agaaattagc aagttattat gatgaagtgg tagggattga tatttctaat caaatgctcg
    15661 atcttgccaa atctaaacgt caactaacaa atacggtcta tctgaatatg aatgcagaac
    15721 aacttaattt taatgagaag tttgatttta ttgtaagccg aacaaccttt catcatttgg
    15781 atgatatagc cagcgtaata cagcaaatga aggaactgtt gaatgaggaa ggaagaatag
    15841 ttattcttga taatgtatct gaagtcgaga caccgcctac ctatgtttat aaattagggg
    15901 caatccaaga gtttttaccg cattgtttca aatttggaat aaaaaatgcg attagaattt
    15961 ataatcacaa cacatctaaa tcatggcttg agcatttagc ttcagataag tacctatctg
    16021 aacaaaatta ttacgattta tatgaaaagt tgttgcctgg atgccaattc cataaaatgg
    16081 gttgggcaat gggggttgtc tggacaaaat aacttaaagc tgtggctttg aaaagttgaa
    16141 tttgtgattg ttttcagagc tatcgggtgc tttagtataa ataacaatga ataaaaaatc
    16201 agcctgtatc ttatgttact attaagtaat ttaagataca ggttttacta tttttgaaat
    16261 aagtaccttc cacttcaaat acatgtattc aaagtgaaag tattgattta aatgattctt
    16321 ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat tttaccaaaa
    16381 ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac
    16441 gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct
    16501 tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg
    16561 aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg
    16621 gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg
    16681 atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca
    16741 aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc
    16801 agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt
    16861 gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac ctgccacaag
    16921 agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa tttaagaggc
    16981 tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt ctcatgtttt
    17041 tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct tatttattct
    17101 tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg ttcgaataag
    17161 aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca gtcaggtcca
    17221 ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat catttctcct
    17281 gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa gatttctttt
    17341 ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag caggtgacgg
    17401 tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata cacatctcca
    17461 gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac atcgccattt
    17521 tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga agcatgtact
    17581 ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat caaacgaagc
    17641 attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt gatttttccg
    17701 tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac acttgttgta
    17761 ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat ttggatttga
    17821 cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg agaacctgta
    17881 cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc tacaaatgtg
    17941 ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt ttccatgctt
    18001 cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc ttgctgcaaa
    18061 cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt tgaaaagatc
    18121 ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat ctcagcattt
    18181 gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat tttatgcaag
    18241 tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc tacgatattt
    18301 ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt cgttctgaaa
    18361 aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc atggagctct
    18421 gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa tgcgagagga
    18481 ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc gatgtggtct
    18541 tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat gatgtagctg
    18601 tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt gatcccggca
    18661 tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc gatttcacct
    18721 aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt catattaaaa
    18781 ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc cttctagaag
    18841 ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag cgtctacatc
    18901 gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc gtacttttgt
    18961 ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt aaagcagtga
    19021 tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc tggtttttca
    19081 tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt ttgcttatct
    19141 gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat taattgttat
    19201 tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa aaatcaatgt
    19261 ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta ttaataggct
    19321 cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga gatgagaaag
    19381 tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc aatttcatat
    19441 gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca cgtatgatcg
    19501 atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata tttatgatcg
    19561 ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt tcgaggatta
    19621 cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa agttattcgc
    19681 atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca aacgatgctt
    19741 atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca ccaatgatgt
    19801 tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg tatgtagtag
    19861 gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct gaaccgttat
    19921 ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa aatctttcag
    19981 gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt tttgctgtag
    20041 tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct gttgcacaac
    20101 cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc ggggcagtgc
    20161 aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag tatatattcc
    20221 atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca agagcagctg
    20281 tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta gaagatccaa
    20341 atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta acatttgctt
    20401 atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc tctccaggag
    20461 agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc caattgatcc
    20521 ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac gtgagagatt
    20581 atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg ctgctattta
    20641 ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa gaagaaatgg
    20701 aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca gatggattta
    20761 atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa cgattggcaa
    20821 tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc ttttctgcat
    20881 tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg acagaatcta
    20941 ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag atcatcgtat
    21001 taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa aaatgcccga
    21061 tttattatga tattgccgct tctcaattat caaaggagga attagcatga aaaaaacctt
    21121 ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt ttcttttagt
    21181 gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg ttacaggact
    21241 tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt tttcttatat
    21301 attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt cgcaattact
    21361 ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga gaagagacat
    21421 agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc aaggaaatat
    21481 tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac aagcttttat
    21541 tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt tttatatcca
    21601 gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga tctcaaaacg
    21661 agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg gtgagctaaa
    21721 cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg gacgcgaaaa
    21781 agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg ggtttcgtgc
    21841 agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg ctacttatat
    21901 tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg tcggtcaact
    21961 acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg tgacacaatt
    22021 gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga ttttagatga
    22081 gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt taggagatgt
    22141 gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc aagatttgaa
    22201 ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg ccggcaagac
    22261 gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa taaggattga
    22321 tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg gtatggtctt
    22381 gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg gaaagcttga
    22441 tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc attttatccg
    22501 aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg tgtctttagg
    22561 acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa tcttgatttt
    22621 agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa aagcaatgga
    22681 tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga caatccgtga
    22741 tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa cgcatgagag
    22801 tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg ctgaggaagc
    22861 agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc tattcttgat
    22921 aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag tttttaaatg
    22981 aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt cagacgaagc
    23041 cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg gcagcacaaa
    23101 tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat gtacaataaa
    23161 aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga gcaggtttta
    23221 cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa taggattagt
    23281 ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat caaatgccac
    23341 agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag ctgttttacg
    23401 cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg gtacaatcat
    23461 ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag gacatcagtg
    23521 catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga ccacgatatt
    23581 gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag taacagaagg
    23641 aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga tccgtccttt
    23701 gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg agataggtcg
    23761 gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca taaaataaaa
    23821 aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag agagcttaaa
    23881 aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac ttcatcgatc
    23941 aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt atctttttca
    24001 atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg ttcgcgtgtt
    24061 tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc gccgccaaga
    24121 ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt tgtaccagct
    24181 gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg gacatctgct
    24241 ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc accaggagaa
    24301 ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag caattgggcg
    24361 atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat acggtctttc
    24421 aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc aatgactgta
    24481 ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg taccataatt
    24541 tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca aataaaaaac
    24601 ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa acaaaacaag
    24661 gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga caggtgctgt
    24721 agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag aacagcgtag
    24781 acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa ataaaaagat
    24841 caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct ttgatgcatt
    24901 tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt cagcgcaaaa
    24961 tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct cccatcgaaa
    25021 agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata aaggcggacg
    25081 cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa aaaaagctca
    25141 gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg tttggagctg
    25201 atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt ttttcttaat
    25261 gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc atcgcggaag
    25321 cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac gagtaggtgc
    25381 agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg ttatgcacga
    25441 cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa cattggaaga
    25501 actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag ctaaagttcc
    25561 tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga tgctaatgat
    25621 tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag ccgaaacatt
    25681 aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg attctttgga
    25741 aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta cttctgataa
    25801 aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa aaaatacttg
    25861 gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg catggacgat
    25921 taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa taataactga
    25981 ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa atatggaaaa
    26041 agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg tggaactcgc
    26101 acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata aaaatctgga
    26161 tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc atttgattga
    26221 tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag gtcgacaatt
    26281 gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta cgggtctcta
    26341 tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga atcctgagat
    26401 tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat ggaatcagtt
    26461 gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac gcaaagtgat
    26521 aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta aagaagagcc
    26581 aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta ttctttatga
    26641 acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag aagcgcgtaa
    26701 ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag aatttttccc
    26761 ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac tacattcgag
    26821 aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac agtggtttga
    26881 tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa aatggctgga
    26941 ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa gcaaaccagc
    27001 gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca tcaggagaag
    27061 tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt atcggtaaag
    27121 ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg atcatcttta
    27181 atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt cttccggtaa
    27241 ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg aaagaaggga
    27301 agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta gggcaaggga
    27361 aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa acaaagttag
    27421 aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa ttgaaagaag
    27481 tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt tttcagattg
    27541 gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg acacaagcgg
    27601 atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag agatggcgtt
    27661 ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa gacctgccta
    27721 cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg gatcttttgc
    27781 tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact gtcttaaaat
    27841 tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat aaggcagacc
    27901 agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt tctactcaga
    27961 cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg gaactaatga
    28021 ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag ttgagaagac
    28081 aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa gttaaagggt
    28141 ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt tttttaa
//
LOCUS       DAJSBC010000030        28192 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSBC010000030 DAJSBC010000000
VERSION     DAJSBC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.030"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(203..1102)
                     /locus_tag="ONT65_001421"
     CDS             complement(203..1102)
                     /locus_tag="ONT65_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR2329061.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1112..1468)
                     /locus_tag="ONT65_001422"
     CDS             complement(1112..1468)
                     /locus_tag="ONT65_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2329062.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1823..2119
                     /locus_tag="ONT65_001423"
     CDS             1823..2119
                     /locus_tag="ONT65_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR2329063.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2763..3716)
                     /locus_tag="ONT65_001424"
     CDS             complement(2763..3716)
                     /locus_tag="ONT65_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2329064.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3739..4494)
                     /locus_tag="ONT65_001425"
     CDS             complement(3739..4494)
                     /locus_tag="ONT65_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2329065.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4491..5450)
                     /locus_tag="ONT65_001426"
     CDS             complement(4491..5450)
                     /locus_tag="ONT65_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2329066.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5443..6393)
                     /locus_tag="ONT65_001427"
     CDS             complement(5443..6393)
                     /locus_tag="ONT65_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2329067.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6543..6995
                     /locus_tag="ONT65_001428"
     CDS             6543..6995
                     /locus_tag="ONT65_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR2329068.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7084..9351)
                     /gene="gshAB"
                     /locus_tag="ONT65_001429"
     CDS             complement(7084..9351)
                     /gene="gshAB"
                     /locus_tag="ONT65_001429"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR2329069.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9538..10536)
                     /gene="ftsY"
                     /locus_tag="ONT65_001430"
     CDS             complement(9538..10536)
                     /gene="ftsY"
                     /locus_tag="ONT65_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR2329070.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10547..11356)
                     /locus_tag="ONT65_001431"
     CDS             complement(10547..11356)
                     /locus_tag="ONT65_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2329071.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11365..14946)
                     /gene="smc"
                     /locus_tag="ONT65_001432"
     CDS             complement(11365..14946)
                     /gene="smc"
                     /locus_tag="ONT65_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR2329072.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14966..15652)
                     /locus_tag="ONT65_001433"
     CDS             complement(14966..15652)
                     /locus_tag="ONT65_001433"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR2329073.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16000..17781)
                     /locus_tag="ONT65_001434"
     CDS             complement(16000..17781)
                     /locus_tag="ONT65_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2329074.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17807..18721)
                     /locus_tag="ONT65_001435"
     CDS             complement(17807..18721)
                     /locus_tag="ONT65_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329075.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18748..19710)
                     /locus_tag="ONT65_001436"
     CDS             complement(18748..19710)
                     /locus_tag="ONT65_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329076.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19710..20654)
                     /locus_tag="ONT65_001437"
     CDS             complement(19710..20654)
                     /locus_tag="ONT65_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329077.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20655..21659)
                     /locus_tag="ONT65_001438"
     CDS             complement(20655..21659)
                     /locus_tag="ONT65_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329078.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21927..22169)
                     /gene="acpP"
                     /locus_tag="ONT65_001439"
     CDS             complement(21927..22169)
                     /gene="acpP"
                     /locus_tag="ONT65_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2329079.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22291..23292)
                     /gene="plsX"
                     /locus_tag="ONT65_001440"
     CDS             complement(22291..23292)
                     /gene="plsX"
                     /locus_tag="ONT65_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR2329080.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23341..25377)
                     /gene="recG"
                     /locus_tag="ONT65_001441"
     CDS             complement(23341..25377)
                     /gene="recG"
                     /locus_tag="ONT65_001441"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR2329081.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25494..27176)
                     /locus_tag="ONT65_001442"
     CDS             complement(25494..27176)
                     /locus_tag="ONT65_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR2329082.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27228..27590)
                     /locus_tag="ONT65_001443"
     CDS             complement(27228..27590)
                     /locus_tag="ONT65_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2329083.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27860..28048
                     /locus_tag="ONT65_001444"
     CDS             27860..28048
                     /locus_tag="ONT65_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR2329084.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7798 a         6089 c         4960 g         9345 t
ORIGIN      
        1 tatttttttt tgtgattatc gttcaaaaaa agaatacacg cttgcatcta gtactctgcg
       61 agttacaaat catccgatca aataaacatt cgctgattat tttccgcaaa aaaagcagca
      121 tatccacgat aaatggatct actgctctta cttatcctta tgttttacaa ggtattttct
      181 tttacacatt actaatgctg tctcaatacg gaacgattgt catcgcttgt tcaaattcct
      241 cttgcaaatg ttgtgtttga taggcctgaa aagctgggtg atgggcacgc aagaggacac
      301 gtaatttcat aggatcatag ctgtctagca actgttcttt caagacaaaa tctcgggtca
      361 gctgccattc ataataataa ccaaaaggtc tgcagctgta aatcgtgtga ccgatggatt
      421 tatcttcaaa gcgacgatgc gtatggccaa aaactacttg tttgatattt gaataacggt
      481 caagtaattc tccgaatgat tctgatccta aaaatgcatt taatttgttc caacgctcat
      541 aaggagcaga ttgatacatg ataaatgccc gtttaggaac gaaatgagtg gataaaatca
      601 cctcaagttt ttttgcttcc agctggtcta gcaaattttt tgcagctgca agcatttctg
      661 cattgacctc gagatctgtt ttcccacgct ggatgaaccg atcataccaa tacaaacgct
      721 tcattcgtac gattgcctcg taatctttca attcggaaaa ctgatagtcg taccagccat
      781 tcatccctaa aagaacggtc tgctcattta aagcaatata ccgttcattt aaaaaatgtg
      841 ggtctggaaa atggtccatc atctgttcat tgggcacatc tgccaactcg tgattgccga
      901 aatgaaatgt cgtagataat cctttattga cgaaaaattc gacaacagct aggcaacggt
      961 ctactttatt agccgtatct ccggctagat gaagccgagt gatcttgtgc ttttgcaaaa
     1021 cttgccaaag ctgcatcagc tctgattcgc cgaatttatt gatgtccaca tgtaggtcgc
     1081 tgatgatcgc caattttccc attttatccc tctagtcttt tcgaacaact tgcaagttgc
     1141 cttttctcaa gccgcttggt ccttttaatt gtaaactatt ataagttggt tctaaagcaa
     1201 gtgtggatgg atcatccatg taaagtattg cactgtcttt gattttgatt ggtccaactg
     1261 gtgtatcagc cactacagaa taaagactta gatctgtttg tgtatctttt ttgacaatga
     1321 tcaatcgaaa tgccgtatcc aattggcaac tgacttggct ttctgcaaat ggaccgtcac
     1381 cgttttcgat atctaataaa agttgatcgt ctgcttctaa tttattcttc aatacttctt
     1441 gtgcttcttt tgtcacttct aatttcatct gtatcacctc atgtcataga ataatcactt
     1501 tattcatcag atgcaactaa tttgtctggt tctgactgtt ctcaatttat tggaaagtaa
     1561 gtcattcgtt tattatgatt tctcttatca caaaagagac aactttccct tttcttcgtt
     1621 cagtttcttg aacggaaaaa gacccaaaaa gcaacctgtt atttgtttaa ccttattcct
     1681 ttcttaaata acaaaaaata ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag
     1741 aattactttt tgttcacgga ttattaacaa ctcttcggta gagtaaactt atcaaataaa
     1801 agacttcttt ggaggaatca ctatgaagga atatataaaa aacatctatt ttatcgaaga
     1861 aacacaaaat atcgagggtt cttatattga agtaaaaaca ctctttgtca atgaagataa
     1921 aaccaaagca ttggatattt ataaaaaatt agcttcaaaa aaaacgaata gttttggatt
     1981 gatattaagc gagtataaaa tcaaagcaga agagagttat ttctatcaat tattaaaacg
     2041 gtggagtaag cttcccgccg atttttatcg aaaaatgcag atcatcaact atcaaccgct
     2101 tgctgagaca catgcctaaa tagaggttgt gaaatcagtg ctttgagcta ataccgaagg
     2161 tcagctgatt gaacattgtt tagaagagat tgtgacaact gcgatttgct ttgagtaata
     2221 agaacgctgg acagaaaata gcttctcata ttttctagtc tagtgggctt attgccgaga
     2281 agcagcagat ggaacatcgt ttagaagaga ttgtgacaac tgcgatttgc tttgagtaat
     2341 aagaacgctg gacagaaaat agcttctcat attttctagt ctagtggact tattgccgag
     2401 aagcagcaga tggaacatcg tttaaagaag agatcggtac aaaataaaaa tatctaaatt
     2461 taagttttat ctaagtttcg ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat
     2521 gctgaaaaaa tggatcacac tattttttgc cactccccat tctagaatca acccgtatga
     2581 tgtagataat gaattcacgt tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa
     2641 atcatcaata cgattttacg gatggttttt gataacttct ttgaggatag gctcttgcta
     2701 tttaagaatg taagaaacat tattgttaca tagtatgaga gtttaatcct ctgttattta
     2761 tgctatttaa atgcttgatt cacatcttcg atcatcagtt tcattgattc taaaccgccc
     2821 ccgcttaaat accagacatc tggttcaagg gagatgatct gctgattttt accagcattc
     2881 gtttgtgcta ccagttcatt ggcagaaata tcatctttgc tgtcgtctcc accaatagct
     2941 tttgttctgt ctactacaaa taagatatcc ggattttttt ccagcacata ttcataagaa
     3001 acactttgac cgtgagtgga tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa
     3061 tcatggatga aaccgaatct agaaccagat ccgtaggctg ataattgccc ttcattcaca
     3121 agtgtgacta gtgctttttt gtcagtagct tctgctttct ttttcgtctg atcgattgca
     3181 ctagataatt cctctaattg agttttagct tcttcttgct taccaaagat ctcccctaaa
     3241 gcagtgacat tttgctggaa agattcccat ggatttttcg catctaaaga aaggaaaatg
     3301 gtcggagcaa tttgacttag atctttttga aaatctcttt gtcggccaga aatgatgatt
     3361 agatctggct gaagctgatt aattttttct aagtctggtt cttttattcc acctgctgat
     3421 tctacttttt tataggatga aagatactca ggaaggctgc ttgtagctgc tccaactacc
     3481 ttatctccaa caccaagagc atccatcgta tctaaagaac cattgtcgaa tacgacgact
     3541 ttttctggat tatttggcac ctcgatttgg tctccattac tatctgttat cgtcaatgta
     3601 gtttcagttt gtgtgctgct tttttttgtg tctgcctgac tggtgttgtt gctatttcca
     3661 catgctgcta aaccaaatga tgctagaatc aagataccta ctactaattt tttcatctct
     3721 tctctcctaa tgttagtttt agaaataaag acagaatctg cggccttcaa tctcacaaat
     3781 ccgtatgtcc atttcgtata attcatccaa tacatctttt tttatcattt cttgggtcgt
     3841 tccttgcgta aataactgtc catctttcat tgcaacaatt tcgtctgcat aacttgcagc
     3901 aaagttgata tcatgaagaa caataacaat tgttttcccg atttcttgta ctaaattttt
     3961 taaaatctgc atgatttgaa ccgcaaaatt catatctaga ttgttcaaag gttcatccag
     4021 taaaatataa tccgtgtctt gcgctagcac cattgcaata tacgcacgtt ggagttgacc
     4081 gcctgaaaga gtgtcaatcg attgatccgc tatttcttct aacccaaggt gttccagtga
     4141 ttcctgaacg atttcctcgt cttttgtcgt caattttcct ttcgtataag gaaatcgtcc
     4201 aaattctact aattctttca cggtgatttg gacatttatt tcattcgatt gtcgtaggat
     4261 cgataatttt ttggctaatt cattggattt ccaagccttt acttcgttat ggtctaaata
     4321 gatttcacct gtatcttttg gtatcaaacg actgatcatc gacaacaccg ttgactttcc
     4381 agcaccattt ggaccgataa aagcagtcaa tttcttttca ggaatcgtta actgtacatc
     4441 tttaaccact gattttccat ggtacagttt attgaccttt tgtaaatcaa tcataggata
     4501 ctcttccttt cgcgaatcaa ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg
     4561 acactcactg ttgtattcca gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga
     4621 aacagcatag ctaccagaaa gcttccgata aatagctgac catgacgata agtagaaaag
     4681 acacgataac tgatatttgc gacaataaac cctaaaaaag taactggacc cacaagagcc
     4741 gttgatgtcc ctgtcaaaag actgacacaa cataaaatca aaaattcaaa acgatctgtg
     4801 tctatcccga ggtttactgc ttgttcattt ccgagatgga aaacgtctag cacatgtcga
     4861 tatcgaaata acaggtacca gcagccgata atgatcaaag aagcaggcaa aagatactga
     4921 ctctggatat tgccaaagct tgcaaataat ctgccttgaa gcaaatcata ttcattcgga
     4981 tccatgatga cttgtaaaaa ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct
     5041 gacatcagta ataaaaaaag attcgctctc atttttttca aaagaaagaa aaataacaat
     5101 agactcagcc cgaccatcag cccaacgttc aataagaaaa aaactgtacc tgtctgctga
     5161 agcatcgtca ctcctccgat aaagaagaaa aggaccgttt ggacaaggac atataacgac
     5221 tcaagtccca atactgaagg agtcaaaaac tggttatgcg taattgtctg aaagctgatt
     5281 gtggcactgc tgacagctag cgagacaagg ataaatgcaa aaattttctt actcctcaat
     5341 gcacatgcaa aatgccagtt tccatatgta tggatcgtca agtacattcc tgtaacaaat
     5401 aaaacaagaa caagcatacc aataaaaata gatggctttt ttttaagcat atcgtcgtcc
     5461 ccttatcaga agataaagga agatagcccc accgatgatt ccaacgatca cactgactgg
     5521 tatttcataa ggaaaaatca acacgcgagc gacaagatcg caaaatacta agaacaaact
     5581 acctgctaaa gcagttacgc caattgtttt gtgcacttga tctccatata ttctagagac
     5641 aagattcgga atgatcactc cgataaaagg aatgcttcct accatgatca acatgactgc
     5701 actagccaat gatactaagc caatgcctaa aaactgcatc ttctggtaat ctattcccag
     5761 atttactgca aacgattcgc ccagaccaat gatcgtaaat cggtaagcaa atagccaaac
     5821 caagagcaac aatggaacaa cagcatacaa taactcataa tttcctcgta tcacagtcgc
     5881 aaaattccct tgaagccatg aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa
     5941 agtagcaact gaaccaatga tattccctaa catgactcca atcaacggaa gcatcaattg
     6001 atttttctgg aggaacaatc gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa
     6061 gcaaaatgct gtcaaggatc ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac
     6121 taaaatacct aaacgcgcac tatccatcgt gcccgcagtc gttggtgaaa caaatttatt
     6181 ttgggtcaaa tgttgcatga tcaacccaca aatactactg gtcgctccca caaggatcaa
     6241 acaaatcgtt cgtggcaaac gagtcactag caagatttgt aagctttgcc catctaactg
     6301 gagcagttct gacaattgta cattcgatac tccgatgaac aatgaaatac tacttaaaat
     6361 aactaataat gctactagtc gtccgtagcg catccaatcc acctttctaa ttgagaatga
     6421 ttttcattct tattttaagt gaatcgttga acaaaagcaa ccgctttttc atatgtttta
     6481 ggtattattg ggaatatgct ataatcgtaa gaaagacaaa gagaagaagg agggtatttt
     6541 ttatggcaca agatgatgta aaaagccatt tggataaggg actttatggt acacctttag
     6601 tgaaccctga agaacagcat aaatatttag ggacattcag ggaacgctgc tatcttagta
     6661 tgactgttgc tgaaatgatc caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa
     6721 aacatccaga tgcttccata ctcttgaatg gggctctacc cctttctgtc caaaaccaat
     6781 atatccagct agcaacgaaa aacaatagcc gtttcactgt agtcaacgat tacgtcactg
     6841 caaatcctga ggaattcgga ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga
     6901 ttgatatcga aaaaaaatac ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa
     6961 aaaagaaaaa aagcttctgg gatcgtttat tttaaaacga aaaaacacat aaatttttac
     7021 atgcaaaaaa agcttttgat cagattgaca tcaaatagga caatctgtca aaagcttttt
     7081 cttttaaaat acttcaggat ataacaattt caatacattc atcgtcaatc ttctgccttt
     7141 tcctgcaaaa ggataaacat gcatatgcat ggctggattg aaatttgctt cgatgatccc
     7201 atatgctttt ttatcagttg taggatcgat ttctttatct ggtataatca aatcgatacc
     7261 actaatctta gctcctagtg cttctaccgc agaaacggcg atttttttgt aagcttcaga
     7321 aaattcatca gtcatatcga tcgaatcacc acctgtactg atatttgagt tctcccttag
     7381 ataaacaatt tgattggctt ggggaactga ttcgatcgtc aacccttgtt ctttcaacat
     7441 caattgttct aattcaccta attggatcaa ttctaaaggt gcacgatgat tcgttccacg
     7501 caaagggtca ctattttttt cttcaacaag ttctttcact gttcggatac tgtcaccaat
     7561 aacattagca gggactcgca acatgatggc ttggacttct ccgtcgatca caaagaaacg
     7621 atattccgtt cccggcataa attcttctac taaaaccgaa ctgtcttcac gaaaagcaat
     7681 ggctaaacct gcttgataat cttccagact agctccttcc ttgaaaatcg tgatacctaa
     7741 cccgtaattg gttgatttag gtttgatcac aaatgcctgc tcggcaaatc tcggataagc
     7801 ttttaccgct tcttccatgg atgaaaattc tgcgcctcct ggtacccgga acccagcttc
     7861 ttttaagact tttttcgtta ctgttttgtt ttccatgata agtgggacga tatagctgtc
     7921 cttacttgtc atgtttgcat ttttcacata ttctacatgg tcttggaatt gcagacgcaa
     7981 aaattgatcc gactcatcta atactttgac ttggacacct ttttggatgg catcgaacat
     8041 aaatatctga gtagaaagtt ccatttcgcg aaaaccggcc aactgatagg gacgttcatg
     8101 gctttcttta taatattgat gtcccaatgc cacagcaact tcttgctgac tcgatagctg
     8161 tgcttcttga tagagtttcg ctgctaacgt ttgttcagga tgatccattt gagtcaatgc
     8221 ttcctctact aaataaagag aatccaacca atccaattca accagcattt gcttcattcc
     8281 ttcaagaatt tctctacctt cttgatgaaa ttctgtttga tctgatggtt gttctagtgc
     8341 tactttgtta tttcgagtcg taccttctgc tacccattgg tcacagtctt ctttttcttc
     8401 tgtccataac atgtacatca ggaataattg taagaaccga acttgctctg gattgatacc
     8461 caatcttgca taaggattca aatcgatatt ccgcaattca atataacgaa cacctgttgt
     8521 tgctaaatct gcgactttct ttccgcctcg gaaacgaagc ggtgtataaa attctttctc
     8581 ctccgaaagt ttcccttctt cgatcattcg ctcgatatct gctaaatact gtttcataga
     8641 agcataggaa actttcacat ttggatgatt cgtatagccc agcgcactgt tgcgaatact
     8701 gcgaacaggt tcttgcggat gtgattcatc taaaaagtag ttcttctcac tcattggtga
     8761 agcaccgaat aaataagtga tcatccagcg atagcgcata aagtttctcg ctgttttcaa
     8821 ataaagttct gttttgaatt cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt
     8881 tcttagcaac tcatcgccaa attcaaaatt gaaatgaata ccgcttacca tctgttttcg
     8941 cttcccgtat tcttttgcta aataacgtcg atacaagaca tcttcgaaat tttttaattt
     9001 tgcaataatg atctcttcgt ctttttccgg taaggcaggt ggcatactta atggccagat
     9061 catctcttct ttcggtatag aacgagcagt cacatcataa acagcagcga gatactgaaa
     9121 taattcggga atagaatcgg taacaggggt gatcatctct gtttgtgttt cgctaaaatc
     9181 tgtttggata taggggtgat aggatcgatc gccaaagatt gctggatgat cggtttttgc
     9241 tagatttcct gcaagatcaa cccgctgtcc ttcccgttct ataccaaatc gagcttggtc
     9301 gataaaaggg cgcgcattga catgcaataa taattgctta aaattcatca ttaagtgacc
     9361 tccaattcat atgaatctaa ttataatcaa aaagtctaag aaaaagaacg aaattgtttg
     9421 agaaccgctt acaaataagt ataaaaatga aggcatcaaa aacacagcta tgttaaaaac
     9481 aatcattcaa acaaggagct gcaacttttg tcacagctcc tgctttttag acatgggtta
     9541 ttcttctttt agtaatcctt tgaacaatcc aacgacaaaa tcatttggat caaacggctc
     9601 caagtcatcg attccttcac caagaccgac caatttcact ggtaaatgca attcattacg
     9661 gatagcaagt acgataccgc cctttgctgt accatctaat tttgtcaaaa caagaccggt
     9721 cacatctgtt gtttctttga attgttttgc ttgaaccatc gcgttttgac cagtggttgc
     9781 atctaataca agaagtgttt cttggggtgc ttctggtaat tcccgctgga ttactcgttt
     9841 tattttttct aattcgttca tcaagttcac tttattttgc agtcggcctg cagtatcaac
     9901 tagtaaaaca tctgcctgct ctgtctttgc tctttccaaa gcatcgaata cgacagctgc
     9961 tggatcgcct cctgcattgc cacgaacaac ttcaacacca gcgcgttcgc cccagacaac
    10021 taactggtcg attgctccag cgcggaaggt atctgctgct gccatcaata cttttttacc
    10081 ttcgtttttg aactgatggg caagtttacc aatacttgtt gttttcccta caccattaac
    10141 accgacaaac aaaataactg ttagtccgtc ttcttggata tttagttcat ttacttcatt
    10201 gattccttcc gcttcataga gatccacaag tttctcgatg atcgcatttt ggacttctgc
    10261 cgattttttc gcatttctga gtttcacttc ttgacgtaat gcttcggtaa ttcgtatcgc
    10321 tgtttcaaaa cctacatctg caccgataag tgtttcttct aattcttcaa aaaagtcttc
    10381 atcgactgtt cgaaaattgg caaacaactc attcatccgt tcaccaaatg ttttacgcgt
    10441 cttttctaaa cccttgtcgt acttctcctg tacttcaact tcttctgttt tctcttcgcc
    10501 agtaaaagca cgcttgattt tatcaaaaaa tcccatttga ctccccctac tctaatacaa
    10561 atttttggat cactttggct acgccatttt cttcattgct ggctgtcacg aaatctgctg
    10621 cttctttgac gaaaggcaca gcattctcca tcgctacgcc tagtccagca tattggatca
    10681 tcggtaaatc attttcctca tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat
    10741 cttttgccaa taaagaaata ccataagctt ttgtaacacc ttttggcata aattccaaaa
    10801 gcatacttct tgttttgatg atctcataca tttcatggaa ttctgctggt atttctttga
    10861 ttttaggatc taattcttct tggtcataag cgattactgc cttattaagc acaaattcat
    10921 ctgtaatatc gcttatatcc gctggttgga attgcagcag cttattcaac acattgtaca
    10981 aggattgctt ccttggggca ctaggcagct gtaaaaccac attgtcagac aaaacatcta
    11041 gtggcagatc caattgctga gccagtttgt ataagcgttg gatatctgat acttccatca
    11101 ctttcttttc catgatttca ccagtatcat ttttttgtac caaaccgccg ttgaaagtga
    11161 tgctgtagtc accttcatct accagtccta attcttgcag ataatgcgcc atcgcagcta
    11221 aggggcgtcc tgtacataga acgatcttga ccccttgctg ttttgcttgt gctaatgctt
    11281 gtttattctc atctgaaatc agcttttgtt catttaataa tgtaccgtcc aaatcgattg
    11341 ctactaattt gatcattttc gttcctacac tttcgtttcg ttgattttcg cctcttcatt
    11401 gatatcttcc atccgtacag aaacgatttt tgatacaccg gattcctgca tcgtcactcc
    11461 gtacagtaca tctgctgcga ccatcgttcc tttgcggtgt gtgactacga taaattgtgt
    11521 gtcatcttgg aaatcgctca agtaacgacc aaaacgtttc acgttggctt catctaacgc
    11581 tgcttctacc tcatctaaga tacaaaacgg aacaggacag acacggataa tggaaaagag
    11641 caacgcgatg gctgtaagtg ctcgttctcc tccagaaagc aaacttagac tttgcagttt
    11701 tttgccagga ggttgtactt caatctcaat acctgttttc agcaaatcag ttggttccgt
    11761 cagtactaat tcagctttac cgccgccaaa catatttgga aagacaattt tgaattcttg
    11821 acggatagct tcaaatactt ctttgaatct tgtccgcact tcatcatcca tctcatccat
    11881 cgtttcaaac aattggtttt tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt
    11941 atgtctttca ctgacttgtt cgtattgttc gattgaattt aagtttactg gtcccagttt
    12001 ttcaatctgt tcttttaagg atgaaacctt tgttctactg tcttcgatat cggttgtttc
    12061 ttgatagtct tgggaagctt tctcaaatgt cagctgatat tctgactgca gataggtcaa
    12121 atgattatct aaaatcattt cagctcggtc tttttggact tctaatttgg attgttccga
    12181 cagtcgtgct ttttgctcac gattacgctc tgccaagact gcttcaagct tatcgatctc
    12241 cttttgcctt ctatctcgct gctctttggc ttttgccaac tcggctttta atgtttctcg
    12301 ctgagcagat aattcgttaa tttgtttttc caaactttct tctgtgactt catgatcaga
    12361 aaagtctgct gtcaatgtcg ccaactgctt ttctatcgct tcttgacgtt cggttgcctc
    12421 tgctttttgg actcgggcac cgcggagctg gatctgcaga tgattaaact gctctttcaa
    12481 gacagcaaga tctgcttgtt cttgtgcttt ttgactttgt acttgtgctc ttctggcttc
    12541 catctggtcg ctttcttgac ttaacgattt gatttcttcg tcgatttttt gtcgttgcga
    12601 ttcaagatct gtttgtttgt ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa
    12661 ctgctgcagt tcacgtgtct caaaagttga gatttgcttt tctttttcaa aacgttcaag
    12721 ttcatttatg atgttttgta gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc
    12781 gcctcgtgta cgcaaaactt cttgttcttc ggctaaacga gctgtttctt gttgcagctc
    12841 ctgaactttt ttttcttgtg cttggagttg tttatctgct tcttcaaatt cactagtcaa
    12901 ctgtttcaat tcttgatttt gtacaaacaa actgccttgg tttcctcgtt tattcgctcc
    12961 acctgtcatg gatcctccgg cattcatcac atcaccttct aaggaaacca ctcgatattg
    13021 atagcggatc gtttgcgcga ttgcattggc actagttaag tctttggcta aaaggatcgt
    13081 tcctagcagg ttttgaacca cagtttggat ttgatcggga taactgactt gttcactggc
    13141 aatccctata aacccttcta cagctgctgc ttgtgtcaat atatgagccg gtaattgtct
    13201 tggtttgatt gtcgtcagtg gaaggaatgt tgctcttcct cctctttgct gtttcaaata
    13261 cgtgatggct tgccgagcat ctttttcatt ttcaacgatg acatgctggg ctgccccacc
    13321 taaagctgtt tcgatagcta acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc
    13381 tccgacaata cccgaaagct gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa
    13441 accaaaatag ttttcttgga tttcttgaag gcttttctta cgagcacgta gctgctggac
    13501 ttgattcatt aattgataca ttttaggctg ttcttttgct aatgcttctt ggaacttttt
    13561 cccgttttgt tggatcattt ctaacttctc ttttgtttca gttaaagatg attttagctg
    13621 cgcctgcttt tgtgtcaatt cttctttttg taagatcaat cggtctactg atgcttctac
    13681 ttccgattgc ttggctaacg tctgcttgct ttttgctgtt tcttgtatat attgacgttc
    13741 tagatacttc agttcgtttc ctacagctgc ttgttcttgc atcaaatcaa catattgatc
    13801 tcttaattct gccaataatt ctttcgttga tttttggtat ttttctaatt cttgctgtgt
    13861 ttttatgaga tttgcttcag ctttttggat ctctgtttct ttttctgctg ctgctttcat
    13921 caagctttct tgaagctttt caaaatgttt aacctttttt tgtgcttctg ctaatgaagt
    13981 ttgatattct tgcgagcttt tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg
    14041 tccttctgtc tgcttcagtt tttccgaaag atctaataat acttgctgat ttttttcgat
    14101 cagacgatcg gcttgcgcat tttctttacg ctgcttcgcc aaaatggatt cttgttcttg
    14161 gatcgactca gatagtttgc ctaattctag attgaatttc gcaagctgtg cttgtttgtt
    14221 gtcccaatct ttttttgctg tcttgatttc agcaaccatc aaagagacat cggtttgagt
    14281 cagtgtttct tttagccgta aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt
    14341 taactgttct tccaattcgt ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc
    14401 aaaaagcttc tgttctgctt tctttttccg ctgcttgtat ttcaagacac cagctgcttc
    14461 ttcaaagatc ccacgacggt cttccggttt actactgaaa atcgcttcta cttttccttg
    14521 agaaatgatt gagaacgatt cttttcctaa tccagaatct aagaacaatt cttggatatc
    14581 ttttaatctg cagctttgtt tattgatgaa aaactcgctt tccccagtcc gtcgataacg
    14641 gcgggttaca ctgatctcat tgtactccaa tggcagataa tgatcactat tgtctaaaat
    14701 cactgtgact tctgcaatat tcagctgctt acgggtatcg gaaccggcaa agatgatatc
    14761 cggcattttt ccgcctcgca gacttttagc agattgttct cctaataccc agcggatagc
    14821 ctctgtaata ttgctttttc cgctgccatt tggtccaacc actgctgtga cgctattttc
    14881 aaaatcaatg actgtcttgt ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa
    14941 atacaaagct ttccctcact tacttttaca gtgctgccaa tgcattttcc gccgcagctt
    15001 gttctgctaa ttttttcgat ttcccttgtc cagtgccaat caattgatca tctacataaa
    15061 cttcgatcca aaatactcgt tcatgagctg gaccttcttc attgatcaat cggtattcga
    15121 ttgatacatc acctgatttt tgcaagactt cttgtagttt tgttttatga tccatctcat
    15181 gtgaaaaagc acctgctttg actttaggaa aaatcacttt ttcgatgaat gcatgggtcg
    15241 tgtcgaatcc ctggtcaaga tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca
    15301 atgctggtcg cgtacgtcct cctgaattct cttctccctt gcccaacaaa acatattgat
    15361 caaagtgaca ttctttggca aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg
    15421 tttttgtcaa ttttccttct ggcaaatgag gatataaacg gaataaatac tgagaaacca
    15481 tcagttctaa aacggcatct cctaaaaatt caagtcgctc attatccgat aattgcagat
    15541 tgcgatgctc attcacatag gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt
    15601 gaaaaacgat gttatagttt tcttttagtt cttttgtcag ctggttgtcc attttccaca
    15661 ttccttttat ggatattttt tcgatacttc cttattatac tggttttttg tcaaatttga
    15721 aatggttcgg tcaaaaggta agtaaagctt taataatcct agtgattgac gagaagttgt
    15781 gaaaaattcg tttagtttcg agtatgaaga aaaaattttg tgaaaatggc tttccatatt
    15841 tttgcataaa tttgacttaa taccgagaaa ctggactttg aacaccgttt attggagaag
    15901 tcatgaaatc agcgccttga cctgaatatt agagaaataa acagaaaaat atcaaaaaac
    15961 cgaaaaagga catcgccctt tttcggtcat ttttagtgat tatgaacgtg aatctgctgt
    16021 aacagaaaca tttgcccatg ggtttggtac atcataagac caatcccagt ctttgacacg
    16081 gtcgttgact ggcaatactt cattacggaa caacgttgga atcgcaaatg cttcttcaaa
    16141 tgcatattgt tgccattttt gatatgcttc tttttggtac tcttcatcaa atgctttgct
    16201 tgaactgata tcgttgatca attttgtatt ttcttctgat tcataacgag tgtagttgaa
    16261 cgcagcattt ggtccccata gtccacttgg atttggatca gttcctgtat tccaagcagc
    16321 ttggtaaaca tcaatttcag gatcatcgtt ttctaattta tcatagaaag aattgaattc
    16381 gatcagacga cctgttgtgt attggacatc caaaccaatt tctttccatt gttggatata
    16441 gtaatctgcc aatggttgcg ctgtttcccc accggacatc gaagcaaagt tgattgttag
    16501 tttatttcca tctttgtctt ctcttaaacc atcgtcatcc acatctttgt agccagcatc
    16561 atctaatagt tttttcgctt tgtctagatc ttgtgtgaat cctttcatct catcatcatg
    16621 gtatgactgg aatactggag gaatcaatga agtcgcattg cttctcaaac ctgcatagaa
    16681 acgttcacct actgcattat tatctaatgc atagcccatt gcttgacgta atgatttatc
    16741 agccattttt gaatctggat tgtattccac actctttgta tctgcattcc atttacctaa
    16801 cttgaatcct agataagtat aagaagtttg ttcacggcct aagtttgtat agccagcaat
    16861 ttctttatag gtatcgtaag tatctgttgg catttggaaa atcatatcat ataatttaga
    16921 gttcaatgct tcagttgcac ttgccatcgg tacagatttc atcgtgatct ttttcaattt
    16981 aggcgtacca ttccagtagt gctcgtttgg caagtattct actgattctc ctgcaacgat
    17041 attactcatg taataagggc caaaagtcac tggattttta cgtacttgat cgcttgattc
    17101 catatctttg attgctacac cttctaacgt atgttttggt gctgcatatg accagatacc
    17161 accacccgat tgaagcatgc ttgggtttac ttctttatat tcgatttcaa ctgttttgtc
    17221 gtctattttt ttgattccgg agattgtgtc ggcacttcca tcatgatatt cttccatacc
    17281 tacgatgtta cggaaagtgt cgtcataacg gataccagtg taatctttat tcccaatgat
    17341 ttcgtaaggg aaaatcacat cgtccgccgt tacatcttca ccatctgacc atttgacgtt
    17401 gtcttttact ttgattgtcg cttttttcgt atcttcatct agatcaagcg ttgcagctcc
    17461 gtcgtctgtg attctgaaat cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat
    17521 aaaagctgca tcatatgaat cttggtaaaa ctcccactgg aacaatcctt taaactgact
    17581 gtccgttgcg accgcagctt ctaattcgcc gtcttcgatt gcatcatcat tattttttgt
    17641 tgcaattgga aaagttaatt cctctttgtt attgccgttg cctccagagt tgtttccaga
    17701 ggtatctgta cctccgcccc cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa
    17761 tccaaaaatc gttttcttca ttcctatttc ccccttcgct tctatactag cctaaccgtt
    17821 ggcgcgcatc tgcagaacgc ttgaatgctt gtccaacata attgatactc aacatcaaaa
    17881 ctaaaataag gattgatgcg ggtaaccaga tccattgccg attcactaag acttctccgt
    17941 tacttgcata accgatcaat gtacctaaac taggtacatt tgccggcaag ccgaaaccta
    18001 agaaagtcaa agttgtctcg ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa
    18061 tgattaatga acttagattc ggcatgattt cgcggaacat gattttaaaa tcattggttc
    18121 ccattgtttt tgaggcactg acataatctc ttcttgcttc agataatgtc ttgcttcgga
    18181 ataatcgggc tttagctacc caataaaatg cactcatgat gaatataaag gaccagacat
    18241 tgtatctgga aataatcgat acgaagacga taatgatcag catgatcggt agaatcatga
    18301 taaaatcaac gatccgcatc aagatgttgt caatgatccc tccgtagtag ccagagatga
    18361 tccctaatcc aaccccgatg atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag
    18421 agtttctagc acctatgatc aattgtccta atacatcacg tccgccttca tctgctccca
    18481 gaatgaactt ttgtccttgt gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac
    18541 taacatacat cactttgtct tgatcggtca gcatcgcacc gataaagatc actaataata
    18601 aaaccactag caaacctaat gaaaaaatag caattttatc ttttttaaat tctcgagcaa
    18661 tcattcgtat ccccatagga ggaatacttt cttgaactgc tgtattgttt ttttcactca
    18721 tcgatccatt ctcctttctg attggtttta ttgaatccga atacgcgggt caacgatact
    18781 catgatgata tctgaaagta atgtccccac tagcgtcgcg attcctaaaa tcaatactaa
    18841 ggatgtgatc acggtatagt cccgttggcc gatggaatca ataaatagtt ttccgactcc
    18901 cggataacca aaaatctttt cgataactac agaaccagca atcaacgaag tgatttcata
    18961 ccccatttga gaagccatcg gcaacgctgc atttcggaaa atatgtcgac tgaatacttt
    19021 attcgtcggt acccctttgg aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat
    19081 tacttcactt cgcagatatt ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga
    19141 tggtaacaga atatatttca aacggtctat ccattgagtg ataaatccac cttgcaaacc
    19201 tagtgtttgt gatccacttg ttgggaacca gcctaaacga taaccaaaaa caaatagcat
    19261 gatcaagccg aagataaata aaggaacagc aaaactaaag aagttgtaga ttacgacaat
    19321 cttatccaag attgaatttt gataccggcc agcaagcatc cccagaggca cagcaatcaa
    19381 ataagtaagg acaactgtca agatcgatag atataaagtg ttgatgatcc gttcgccgat
    19441 caagtcagca acagagcgtt tgtataagaa actttgacca aaatcgcctt gcaaagcatt
    19501 tccgatccaa cggaagtact ggacatacca tgggtcattc aagccagctg cttgtcgcat
    19561 ctgttcgatc actgccggat cctgatttgg attgatcaaa ccggtaaatg gatcccccgg
    19621 catcgcttgg gctaataaga agatcagaac acttaaaata ataacttgcg ggatcatcaa
    19681 taaaacacgt cgcagaatcg ttttccacat tagcttgcac ctccatcttt caacgccact
    19741 ttgtgtgtag ttgtgatcgt acgcaaatcg tagactcgac cagtcgcatc atagtattct
    19801 ttttggtttt ggatatactc ttgttctact cttcgtcgat tttctttgtg gctttcccta
    19861 ttttctacat cgatttgcgg aatagctgaa agcagacgtt tcgtatagat atgtcgcggg
    19921 tcattataaa tgtcttctct tgtaccgatt tctacaaatc gacctttgta cataattgcg
    19981 atattgtcgc acatgtgttt tactacacct aaatcatgtg aaatgaataa atagctgagg
    20041 ccgtattctt gctggattcg tttcatgaaa ttcaatactt gcgcttgaac agatagatcc
    20101 agagcagata caggttcatc ggctacgatc agttttggat tggtagcaac agcacgggct
    20161 actcctagac gttgacgttg tccgcctgaa aattcatgag gatatttata tagcgcatct
    20221 tccggcatcc caacgatatc tagcagtcct tttacctttt tcttttcttc ttgatcgctt
    20281 aaccgttcaa aatttcggat gggttcagca atgatatcca acactcgctt tttaggattc
    20341 aaactggaca ttgaatcttg gaagatcatc tgcacatcct tgttataacc catttttttg
    20401 cggttgcttc gcttcgttac atcttttcct tcatacatga tttgtccggc tgttaccttt
    20461 tctaatccca caacggcttt tcccgtagta gattttccag agccagactc tcccactaaa
    20521 ccatatgtct ttcctttttc gataataaaa tccacaccat ctacagcata tacatgatcc
    20581 gttactcgat tgaagaatcc actgcgaata gggtaatgaa ctttcaggtc tttgattgtg
    20641 attaattctg tcatctacac ctctcctttt tcgtctctaa agtggaaatg cttatagcag
    20701 gagcaacgta ccaagtggtt cggtgcgact tcatgaagga taggctcttc ttcatgatcg
    20761 cttgctggta tccatggaat acgtggtgca aaccgacaac ctttacgagg catgtttttc
    20821 agagaaggaa cgacaccttc gatcacatgg agttctgcat catgatttcc ttcttgtggt
    20881 atagagttca gtaaagagcg tgtataagga tgcttaggat gatcaaacag ttcttttaca
    20941 cctgctactt caacgaactg tccgccatac atcactgcca ctcgatcagc catttctgct
    21001 acgactccta agtcatgagt gatcaagatg atccctgttt ttgtttcttc ctgaaggtct
    21061 ttcagtaaat caagaatctg tgcttggatg gttacatcta gggctgtcgt cggttcatct
    21121 gcaatgacga ttggaggttt gcaggcgatt gcgattgcga tgatgacacg ttgtctcata
    21181 ccgcctgata gttcatgggg atattggcgt ccaactctcg caggattagg aatccctact
    21241 tgttctagca attctagtgc acgagcttga cgctgttctg cattcatctt tgtgtgatag
    21301 gaaagtcctt ctttgatctg gtcttcgatt ctcatgagag gatttaatgc agacaacgga
    21361 tcttggaaaa tcattccgat atcatttccg cggattttat tatagagcgt ttcgttcagg
    21421 tcgattaaat tcagatcgtt atataaaatc tcacctgtaa tttttgtgtt attaggatca
    21481 agtaatccca taatcgtagt agccaaggta cttttcccgc acccagattc cccaacgatt
    21541 gctaaaatct cgtttttgtc taaagtaaat gaaacgtcat ctactgcatc gtaatagtca
    21601 tctttgagtc ggaatcctgt atgtaggttc tgaacttcta ataattggtt atcggacaat
    21661 cgaatacccc tctctgcttc ctaaatggaa gtttttctta ataaaaaagg acattacaca
    21721 gaataaatcg ttttataaca aaggttattc ttatctttgt gtaatgaatt attaataatt
    21781 atatagaaaa ccgctttact ttgcaacata atttctgttt atcgccagaa ttatcagaaa
    21841 aatgactttt acgtgtcaaa aagattaaaa aaagaaggat aaaaagctgc atgcagcttt
    21901 ttatccttct ttgcttttat ttgaaattat ttaagatggc tgctgatata atctactgcg
    21961 ccaccgactg tttcaatttg ttcagcgtct tcatctgaaa tttccgtacc gaattcatct
    22021 tcaagctcta aaacgaattc cataatacta attgaatcag cttttaaatc atctttgatg
    22081 ttcaattcat ccgtcacttt atcggtatct atctcaaaat gatttgaaat aattttcgct
    22141 actttgttaa atacttcttc gcgagtcaac tttttcacct ccaagaatca tacacttgtc
    22201 aattacctac accatggcta attttactgg catttttcag gaatgtccac ctttttttca
    22261 atggaacgga tcgcttcaga tatccaaata ctattcttcg cttttttcaa attgttcgac
    22321 taattgtcca actacatctg tctcaagcat tgtgtggatt tggcggatcg tatagcgaac
    22381 agcatcgggg cctgttgctc catgagtttt aatgactggt gctttcaagc caaacaaaac
    22441 ggcaccgcca tgtttggaat aatccatttc tgctttcaaa gaacgtaagc cgtcttttaa
    22501 aagcatagcg cccatttttc ctttgatccc ttcatttaga atagcagatt ttaatagatt
    22561 catcatattc attgctgttc cttcaataga tttcaaaaca gcgttgcctg taaatccatc
    22621 tgtgaccact acatcagcca caccattgag tagctcacga gcttctacgt ttccaataaa
    22681 attcaagctt gtttcattct gaaggagttc ataggctttt ttcgtcactt cactaccttt
    22741 tgttgcttct gttccgttgt ttaagagtgc gacacgagga cgtccgatac ctcggacttt
    22801 acgtgcataa aaagaaccta aaataccgta ttgcaacaaa tgttctggtt tgttttctgc
    22861 gttagcgcca agatccagca tatcgaaacc ggcaccttct ttccctacta ctggcaaggt
    22921 agacatcaag ccagggcgtt cgatattttt gatacgtcct acgatgaaca accctgctgc
    22981 tagtagcgcg cctgtattcc ctgctgaaaa tacagcatct gcttcaccgt ttttcactgc
    23041 ttgtgcagca aggaccattg atgcagtctt ttttcgtcga attgctttta ctggctcatc
    23101 atcactattg attttttcgt ctgtatggat aatcgtgatg tttttttcat ttgttaagta
    23161 tttcttgatt tctgcttctt ttccgtaaag taagaattct acttctggaa aatcttgctg
    23221 cgctaaagcc acgccttcta caattgcttg aggtgcgtga tctccgccca ttgcatctac
    23281 ggcaattttc attgtttcca ctcctttttc cgtcatcagt ataaagtata gcacagtctg
    23341 ctaatcgaaa taacgattat cctgattttc tatttggagg ctttgcgcta aaggctgata
    23401 ctcaggaagc atctgccaat cctttatttg ccaaagggct gcagcctctt ctctggctac
    23461 ctccaaaaca tttccatccg tcaccaagtc agcaatcgca aattgaggca gaccagattg
    23521 acggaagcca aatacttcac caggaccacg taattccaga tctttttcgc ttagtacaaa
    23581 accattggtg gtctctgtca tgattttcat ccgttccaca cctagttcat ttttaggatt
    23641 agcaactaag atacagtagg aagcatcact tccgcgtcca acacgtccac gaagttggtg
    23701 cagctgtgcc aatccaaatc gatccgcatc catgatcagc atgacggtcg cgttcggcac
    23761 gtttacccct acttcaatta cagtagtcga aactaatatc tgcatctggt tgtctttgaa
    23821 ggcttccatg attacttctt tttcctgatt cttcatttta ccatgtaaca agccaacttc
    23881 atattttggt gcaaatagtt cacgaagttt ttcatagatt tccactgcat tttttacatc
    23941 taatgcttct gattcttcaa tcagcggaca gatgacatac atttgatgtc ctcttgccag
    24001 ttcttttttc gtccaatcca gtacactgtc caattgaggt gttcttaccc agcgtgtttc
    24061 gatcgggata cgtccagctg gaagttcatc aatgatagat acatccatct caccatatgc
    24121 tgtaatagct aatgttctag gaatcggagt cgctgtcata aataagacat ctggtcgcca
    24181 gcctttttca cgaagtactt tccgctggtt cacgccaaaa cggtgctgct catccgtaat
    24241 aactagacct aaattctgaa attcgactcc atcttggatc aatgcatgtg tgccgatcac
    24301 gacgttaatt tccccttgct ctagtttttc aaggagctca cgtcgttctt ttgctttcgt
    24361 tgaccccgta atcaatgcga tggttacttc attgggatca aacagctgtt gcaagctttc
    24421 catatgttgt tgtgccaaga tttctgtagg caccatcaac gcaccttgga aacctgctgt
    24481 aacggtagcg tataacgcaa tcgcggcaac cactgttttt ccgctaccta catctccctg
    24541 caaaagtcgc tgcatatgct gcggactgcg aagatcccga cagatttcat tcgttacacg
    24601 tttttgtgca tttgtcagtt caaaaggtag cttttgagta aattgtttca atttttggac
    24661 atcataaagg atctccaaac cattcgtttc tgctttttct gcttttttca atccttgaag
    24721 acgcatttga aataaaaaga actcttcaaa aacgacacgt cgtttggctt gatggttttc
    24781 ttcgtggtct tttggaaaat gcatacttct tacagcagaa gctcggtcaa gcagtcgata
    24841 tttctccaca aggttgtttg gcagattttc ttcaatcaat gtgccgtatt cttgaaaccc
    24901 ttttttgatt agatcaacta atgtcgtttg acgaattgat ttgttgacgt gatatatcgg
    24961 cgaaaagtca tccattgttt gagaacctaa aatcttcatc ccgttcaagg ctttacgctt
    25021 tgcatcccat ttaccgtata cggcgatttc ctccgataaa atgacttttt cttttagata
    25081 aggttgatta aaaaaagaaa cgttgaacac atcatgttct tgcatcatac gaaattgcaa
    25141 tcggcttttt ttataaccaa atcgattcat cacaggaggc gagactacga ttcctttcaa
    25201 agtaaccttt tcttgatcct ggatctcgtt tagatttctt tcctgaatat cttcgtagcg
    25261 aaaaggataa taagtcagta agtcttcaat cgtttggatg cccaagctgg caagactagc
    25321 tgctcttttt tctccgactc ctgccagcat agtgattgga tcatctaaag aacgcataaa
    25381 tttttcctcc ctcctttaac aaaaaaagga cttggtcagc gatcatacct gatcactaac
    25441 cgaaggcctt ttatgaatga atactgtgtt agaaagatat tctgacacag aaattattca
    25501 gcagaaaata gataaggata gacaggctga tctccttggt ggatctcgat ttccaaatca
    25561 ggatgttttt ctgttagttt ctcagcaaat tcatttgctt gttcaactgt tccttcatca
    25621 cctgccaaga tcgttacgat ctctgtgtcc tcattgatca tttgttctag tgtacttaac
    25681 gctgttgctg tgatatctgg gttagagatc aggatttttc catcgatcat gcccaaaaag
    25741 tctccttctt tgatctctac accgtcaatt gaggtatctc tgatcgcgtg agtgacttga
    25801 ccgctcacaa cgctagacaa catttctgtc atcgtttctt tgttttcttc taaagaagct
    25861 tgatcattga atgaaagcat agctgtcaat ccttgggaaa tcgttcttga aggaacaact
    25921 gctactggga tatctgctac ttctgctgct tgatcagctg ccatgaagat gttcttattg
    25981 tttggcaaga caatcacttt atctgcgttt acttcttgaa tcgctttaac aatgtcttcc
    26041 gtacttggat tcattgtttg tccaccactg ataacataat tagcgcctaa actttcaaac
    26101 agctcttgga cgcctttacc agcagcgatc gctacgatac catacgggat acgtggtatt
    26161 tcttttttcg caaactcgtt cacttcttca tcgtgttcca aaatcgtctc atgctgcaag
    26221 cgcatgttgt cgactttgat tttgactaat gaaccgaatt tttgtccata attcaagact
    26281 tctcctgggt tttctgtatg cacgtggact ttgatgatct cgtcatcatt gaccacaagt
    26341 aaagaatctc ctataccatc taaatagtta cggaattctt cgtaatcgaa tgtactatcg
    26401 actgtaggtc cttctccgat ttttaccatg atttctgtac agtaaccaaa atggatatct
    26461 tctgtcgcca tttgaccttg gacactgcga tgatgttctg cattgaccat atcatccatt
    26521 tctgctggag aaggttcata tgtttcatcc gcttggaatt ctccattcaa tgcacttaaa
    26581 aagccctcat aaacaaacag cagtccttgc cctccactgt ctacgacacc gacttctttt
    26641 aggactggaa gcaagtctgg agttttagct aaagcacgct ttgctccttt tacaacagct
    26701 gtcattactt caacgacatc atcagtactt tttgctttct tttctccagc tttcgctgct
    26761 tctctagcta ctgtcaaaat cgttccttct actggtttca ttactgcttt atacgcagtt
    26821 tcaactccat gagtgaaggc tgcagcaaga tcagaagcat ttaatgagac aacatctgga
    26881 atttgcttag aaaatccacg aaacaattga gacaaaataa cgccggaatt tccgcgcgca
    26941 cccatcagca atccttttga aagcattgct gctaattctc ctactttttc tgaggttgaa
    27001 tctactactg ccttagctcc actagtcata gatagattca tatttgttcc cgtatcgcca
    27061 tctggcacag ggaatacatt taatgaattg acgtattctg catttacttg cagacgattc
    27121 gcacctgcct ggaccatttc ctggaactgg cttgcactta tttctgttac actcacctag
    27181 ataatcctcc ttcagatcga ctgctgagtt accagccagt ctcctgccta gtctggcagt
    27241 acacgaacac cttgtacaaa gacattaact gaattggcag ttacgcctaa taatgcctct
    27301 agattgtatt tcactttttc ttggacgttg cgggaaactt cagagatttt cgtaccatag
    27361 ctgataatcg tataaacatc tacagcaata ccattatctt cttggcggac aactactcct
    27421 cttgagtaat tttctttacg caaaatctca gtaatattat ctttaatttg atttttgctg
    27481 gccattccta caacaccgaa cacatctgtc gcggcgccac caacaactgt tgcaattaca
    27541 tcattggtaa tttcaatggt tccagctgac gtttgaattt ttacagccat gaatgagcct
    27601 ccttaacggg taaaaaactc ccatttttct tctatcatat tttatcatag cgcaacttga
    27661 aataaaaggc agttacccaa agtactcaaa aaccttgcaa aatttcaggt ataaccttgt
    27721 ttaatttaca attattcttc tcaattgtca attgtatcta tgaaaatgta cttgcaaatt
    27781 cccattctat atgatatgtt agtctggtat gagctgaatt attctattgc tccaaatcaa
    27841 ggcaaaggag gaaattacca tggcaaaagt ttgttacttt acaggtcgca agacaaaaag
    27901 tggaaacaac cgttcacacg ctatgaactc aacaaaacgt actgttaaac ctaacttaca
    27961 aaaagttcgc gttatggtcg acggaaaacc taaaaaagtt tgggtgtcaa ctcgtgcttt
    28021 gaaatcagga aaagttgaac gcgtttaatt gaatgatact tggaccgttc agatagtgaa
    28081 cggtttttta tttaataaaa atttttaagg gtgggacttt tgattcctac cctttttttg
    28141 cgtttatttt tgttatacga acaaattatg aagttattta ttctaaattt ta
//
LOCUS       DAJSBC010000031        27900 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSBC010000031 DAJSBC010000000
VERSION     DAJSBC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.031"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(418..1362)
                     /locus_tag="ONT65_001445"
     CDS             complement(418..1362)
                     /locus_tag="ONT65_001445"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2329085.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1658..2602)
                     /locus_tag="ONT65_001446"
     CDS             complement(1658..2602)
                     /locus_tag="ONT65_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329086.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2823..4037
                     /locus_tag="ONT65_001447"
     CDS             2823..4037
                     /locus_tag="ONT65_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329087.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4059..6074
                     /locus_tag="ONT65_001448"
     CDS             4059..6074
                     /locus_tag="ONT65_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR2329088.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6157..7530)
                     /locus_tag="ONT65_001449"
     CDS             complement(6157..7530)
                     /locus_tag="ONT65_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2329089.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7556..7840)
                     /locus_tag="ONT65_001450"
     CDS             complement(7556..7840)
                     /locus_tag="ONT65_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2329090.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7842..9857)
                     /locus_tag="ONT65_001451"
     CDS             complement(7842..9857)
                     /locus_tag="ONT65_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2329091.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10036..10341)
                     /locus_tag="ONT65_001452"
     CDS             complement(10036..10341)
                     /locus_tag="ONT65_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR2329092.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10430..11461)
                     /locus_tag="ONT65_001453"
     CDS             complement(10430..11461)
                     /locus_tag="ONT65_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2329093.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11668..12543
                     /locus_tag="ONT65_001454"
     CDS             11668..12543
                     /locus_tag="ONT65_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2329094.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12695..13537)
                     /locus_tag="ONT65_001455"
     CDS             complement(12695..13537)
                     /locus_tag="ONT65_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2329095.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13629..15506)
                     /locus_tag="ONT65_001456"
     CDS             complement(13629..15506)
                     /locus_tag="ONT65_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2329096.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15503..16924)
                     /locus_tag="ONT65_001457"
     CDS             complement(15503..16924)
                     /locus_tag="ONT65_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2329097.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16927..20316)
                     /locus_tag="ONT65_001458"
     CDS             complement(16927..20316)
                     /locus_tag="ONT65_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR2329098.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20573..21964
                     /locus_tag="ONT65_001459"
     CDS             20573..21964
                     /locus_tag="ONT65_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329099.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22037..22684)
                     /locus_tag="ONT65_001460"
     CDS             complement(22037..22684)
                     /locus_tag="ONT65_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2329100.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22692..23378)
                     /locus_tag="ONT65_001461"
     CDS             complement(22692..23378)
                     /locus_tag="ONT65_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329101.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23384..24457)
                     /locus_tag="ONT65_001462"
     CDS             complement(23384..24457)
                     /locus_tag="ONT65_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329102.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24679..25353
                     /locus_tag="ONT65_001463"
     CDS             24679..25353
                     /locus_tag="ONT65_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2329103.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25358..26698
                     /locus_tag="ONT65_001464"
     CDS             25358..26698
                     /locus_tag="ONT65_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2329104.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26847..27833)
                     /locus_tag="ONT65_001465"
     CDS             complement(26847..27833)
                     /locus_tag="ONT65_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR2329105.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8136 a         5269 c         5056 g         9439 t
ORIGIN      
        1 gataaacctc tctcgaacgg ttttttatca aaaatcgtca tttttccatc aaaatctgct
       61 tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt ttaattgaat
      121 gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat atgaatcatt
      181 gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga taacgattcg
      241 cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca ttttcggacc
      301 gattaatttt aaccattctg tttatttgct tacctattag atatcaataa aaaactgctt
      361 aacagctatt tcagctgtta agcagtttta ggatttttag ttcatattca ttgtttctta
      421 taatacagtg ttcaatactt ctcttaaagc acttgttgat ttgctgaaca ttgctttttc
      481 ttcttcgttg attgataatt caacaacttc acgaacacca ttttcgtcaa cgattgatgg
      541 cacacctgta aagatatctt tttcgtcgta ttcaccagtt aaataagcag atacaggcaa
      601 tacagcttgt tcgttattca agatagcttt tacgatacgt gcagtactca taccaatacc
      661 atagtaagtt gcttttttac gatcaatgat ctcataagca gcattacgga ctttatcagc
      721 aatcacatct aattcatctt gggcaatacg atggtctttt tctacaattt caaacactgg
      781 tttaccaccg acagttgtat gtgaccatgc agcaacttct gaatcaccat gttcacctaa
      841 gatatagccg tggacgctgc gtgggtcaac ttttaatttc aatgcgattt ctttacggaa
      901 acgagttgta tccaatgttg ttccagtacc gataacacgt gatgttggca gaccagattc
      961 gttccaagcg atatatgtca aaatatctac tgggtttgaa gcaacaacga taataccgtc
     1021 gaatcctgat cccatgattt cttttacgat ttggcgcatg attgacgcat ttgttttaac
     1081 taaatctaaa cgagattgac caggtttttg gttgatacca gctgtgataa caacgatatc
     1141 agcatcttta cattcttcat agccacctga ccatacagcc acattttcat cgccccaagc
     1201 cataccatct aaaaggtcca atgcttcccc ttctgctttt tcttgattca catcgatcaa
     1261 tactaattca ttagagattc cttggttgat cattgcataa gcgatacttg ttccaacaaa
     1321 acctgtaccg acgattacta ctttacgact tgtttttttc atgaataaac agctcctttt
     1381 cataactatt tgttactttc atgaaaagta tagtcttgtt tattcatttt atcaacatgt
     1441 taattataat agttgttatt ataatttttg atggaaacga attcctgaat acgcataaca
     1501 gataaatgac aaatgtttac acctatatcc cctgataata caaccaaaag tttagaactg
     1561 aaaataaatc caaaaaaatt cgctgcaacg atataataag acagcaatcg cacctaaaac
     1621 aattttcctt catatgcttt agtgaaagat tttcttctta atgtttattt ctccattcat
     1681 tgggagtaca tgttgaatac ttcttgaata atttgaataa agaagtcgtt tgtttatatc
     1741 cgatacgatt agcgatttct tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa
     1801 agtcgaattt tttctttaga accagttctt gaaaggaagc atcaaaatac attttcaatt
     1861 tattactaag atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag
     1921 aagtaaaatg cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat
     1981 ctgctgatga aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg
     2041 tatagagatt taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat
     2101 tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat
     2161 gttccccttt ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg
     2221 ccaaatgatt caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa
     2281 tatcctcttg ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt
     2341 cactttcttc catctctatc ctttctccat taataatttg taaacatgta ccgctataca
     2401 tataattgat ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt
     2461 tactgatatg aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct
     2521 gaagatggta agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt
     2581 cgattgattc ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa ttgactccct
     2641 cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa
     2701 gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc
     2761 tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga
     2821 acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga
     2881 cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt
     2941 ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt
     3001 tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag
     3061 tgattgcatc gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag
     3121 cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga
     3181 ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc
     3241 aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc
     3301 aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg
     3361 ttcctacacc tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa
     3421 tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat
     3481 ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa
     3541 ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt
     3601 ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg
     3661 gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga
     3721 tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc
     3781 aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag
     3841 gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa
     3901 cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc
     3961 tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac
     4021 aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag
     4081 atagatggct ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc
     4141 aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga
     4201 acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc
     4261 aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt
     4321 gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga
     4381 tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg
     4441 aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa
     4501 tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct
     4561 tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata
     4621 tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt
     4681 gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct
     4741 atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt
     4801 tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt
     4861 cagtatcaga agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac
     4921 gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt
     4981 tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca
     5041 agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc
     5101 actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat
     5161 taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag
     5221 attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag
     5281 tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat
     5341 atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac
     5401 aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat
     5461 ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta
     5521 acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga
     5581 ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga
     5641 atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa
     5701 ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag
     5761 atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac
     5821 gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta
     5881 atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc
     5941 ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa
     6001 tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa
     6061 aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc
     6121 tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat
     6181 gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat
     6241 gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg
     6301 tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg
     6361 aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc
     6421 ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg
     6481 tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag
     6541 tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa
     6601 aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg
     6661 gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc
     6721 agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag
     6781 ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat
     6841 caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat
     6901 gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata
     6961 accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa
     7021 cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa
     7081 gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga
     7141 aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag
     7201 taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg
     7261 caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt
     7321 ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag
     7381 tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt
     7441 ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat
     7501 tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt
     7561 cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca
     7621 aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga
     7681 agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact
     7741 cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac
     7801 ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt
     7861 atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta
     7921 ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca
     7981 gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt
     8041 tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt
     8101 aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata
     8161 gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg
     8221 attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat
     8281 tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt
     8341 aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc
     8401 gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt
     8461 acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga
     8521 gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt
     8581 tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat
     8641 aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc
     8701 acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat
     8761 agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac
     8821 ggaagtaatg cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg
     8881 atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga
     8941 taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc
     9001 atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca
     9061 tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt
     9121 tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc
     9181 aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa
     9241 ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa
     9301 atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt
     9361 aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt
     9421 gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac
     9481 gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact
     9541 gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc
     9601 gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat
     9661 cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata
     9721 cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt
     9781 acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa
     9841 ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt
     9901 ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac
     9961 gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga
    10021 attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt
    10081 ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg
    10141 ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc
    10201 accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc
    10261 tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt
    10321 ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac
    10381 aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc
    10441 cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt
    10501 cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga
    10561 cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat
    10621 ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag
    10681 gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc
    10741 ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta
    10801 tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt
    10861 tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa
    10921 cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat
    10981 gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat
    11041 cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc
    11101 tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg
    11161 cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg
    11221 ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct
    11281 caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc
    11341 acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg
    11401 aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca
    11461 tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt
    11521 tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga
    11581 aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc
    11641 agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag
    11701 ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca
    11761 gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt
    11821 cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg
    11881 gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca
    11941 agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata
    12001 aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac
    12061 aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc
    12121 cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct
    12181 tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt
    12241 cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg
    12301 ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata
    12361 tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat
    12421 tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc
    12481 aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact
    12541 taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat
    12601 catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg
    12661 attcgaatag atatattata gaaatgagga atagctaact tctgtttctt attttgaacg
    12721 attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat
    12781 acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat
    12841 gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac
    12901 aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg
    12961 gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat
    13021 agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc
    13081 ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt
    13141 ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat
    13201 ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat
    13261 cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt
    13321 ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat
    13381 tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca
    13441 gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca
    13501 tcagcagatc tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg
    13561 tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg
    13621 caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga
    13681 aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta
    13741 cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat
    13801 tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc
    13861 gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt
    13921 gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat
    13981 gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct
    14041 ccagcaagtg tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca
    14101 gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct
    14161 tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat
    14221 ttcaatgtac cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat
    14281 ccgtctgttt gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat
    14341 aaggcataag catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta
    14401 tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat
    14461 gggatattga cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc
    14521 aattgaggtg ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt
    14581 gtttggctat ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca
    14641 ttatcaggag cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc
    14701 aactggcctt tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga
    14761 ccagtaatga aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg
    14821 acactttgga tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct
    14881 gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca
    14941 ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta
    15001 taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg
    15061 gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt
    15121 tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact
    15181 ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta
    15241 tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat
    15301 ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt
    15361 aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact
    15421 ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata
    15481 acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc
    15541 agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt
    15601 ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt
    15661 ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta
    15721 aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg
    15781 aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg
    15841 tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga
    15901 atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac
    15961 tccctgtata tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt
    16021 tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg
    16081 tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt
    16141 gaggtttctt gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta
    16201 aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat
    16261 agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg
    16321 taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt
    16381 cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag
    16441 gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa
    16501 tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga
    16561 taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg
    16621 caccgttttt tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat
    16681 ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg
    16741 tttgtaataa agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg
    16801 ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct
    16861 gagtaaaagc tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt
    16921 tcatatttag ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca
    16981 accaatcatc cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg
    17041 tacttttgcc tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag
    17101 aacagattca tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt
    17161 aactggctct tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt
    17221 tccaggtttt aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc
    17281 taagtccatt tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc
    17341 ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca
    17401 tacattattt gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat
    17461 ttggaattca taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac
    17521 tcgatattta ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg
    17581 gctgtcaatt ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc
    17641 tttaagattt atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac
    17701 ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt
    17761 tccatcaatg gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct
    17821 taatccatgc ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa
    17881 tttgacattt tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat
    17941 agaatctcct gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt
    18001 tttcgttatt ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc
    18061 tatttgttga gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc
    18121 ttgaccttga ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt
    18181 tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt
    18241 aatgattcga atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat
    18301 ctcaaaagta acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt
    18361 tttgatgtga agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc
    18421 attggtatca atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg
    18481 atggaagtcc tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc
    18541 ttttcctaaa tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt
    18601 gaccgttgta ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa
    18661 tggttctgcg attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc
    18721 ttttttggct aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc
    18781 gcctttttca tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag
    18841 accagctgcc ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc
    18901 tttgagtgcc atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag
    18961 attgttttgg tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc
    19021 ttgcgtatta gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg
    19081 tactttatag gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc
    19141 atttggaacg gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc
    19201 atttgtccgt gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat
    19261 ctgtactgca ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt
    19321 gattttgtct gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt
    19381 tacttcaccg atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag
    19441 atcaagaggc gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa
    19501 tccgggtgta cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt
    19561 tcctccatat tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat
    19621 accatcccat tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc
    19681 tgtaggttgc caactggctt taggataagt gccctgatca tctgttgtat attctgtagg
    19741 aatcgtttga ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg
    19801 gctgacagaa ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac
    19861 tgacacatct gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc
    19921 gacgatcagc ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc
    19981 gatagtttgt ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata
    20041 agcatagcca tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat
    20101 ttctttttct atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt
    20161 ttttccagac tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc
    20221 tgtagtgtct gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat
    20281 gaaaataact ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt
    20341 ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta
    20401 ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc
    20461 aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag
    20521 ggttgaggga gaaaaacata tgatcaggtt gttggaagga aactatagaa agatggaaaa
    20581 aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg
    20641 aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg
    20701 aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt
    20761 accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt
    20821 aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat
    20881 cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt
    20941 attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt
    21001 gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc
    21061 aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt
    21121 tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat
    21181 gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat
    21241 aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag
    21301 cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa
    21361 agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc
    21421 cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt
    21481 tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc
    21541 acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt
    21601 ttattccact ctactgaatc agaatctgat ttattattta tctagctata tcttttggat
    21661 gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag
    21721 cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt
    21781 gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct
    21841 tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat
    21901 attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt
    21961 atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac
    22021 acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt
    22081 cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta
    22141 cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat
    22201 gattgattct taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc
    22261 tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga
    22321 tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat
    22381 gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt
    22441 tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg
    22501 acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa
    22561 atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag
    22621 tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag cccttcattg
    22681 tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga
    22741 taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt
    22801 tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc
    22861 aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca
    22921 attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac
    22981 tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta
    23041 acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt
    23101 tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca
    23161 gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa
    23221 atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct
    23281 tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat
    23341 gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg
    23401 taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa
    23461 tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc
    23521 ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc
    23581 caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg
    23641 ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga
    23701 ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg
    23761 cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg
    23821 atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt
    23881 cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg
    23941 catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag
    24001 gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat
    24061 tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta
    24121 ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga
    24181 ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc
    24241 ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt
    24301 gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg
    24361 acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta
    24421 atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga
    24481 ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact
    24541 tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat
    24601 gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca
    24661 aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc
    24721 aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt
    24781 gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg
    24841 ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca
    24901 gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt
    24961 gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca
    25021 ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt
    25081 tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa
    25141 gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag
    25201 atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat
    25261 atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta
    25321 cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa
    25381 gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt
    25441 gggattcatt actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt
    25501 agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg
    25561 cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt
    25621 aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga
    25681 gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga
    25741 tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc
    25801 cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat
    25861 ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat
    25921 gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt acttgtctag
    25981 tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc
    26041 ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac
    26101 caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac
    26161 gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg attctgatca
    26221 gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc
    26281 atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt
    26341 agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc
    26401 attaaaatat acgaatccag aagataagat cattttggat tcatctgtta accataaaga
    26461 atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat
    26521 ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct
    26581 tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa
    26641 ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg
    26701 cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata
    26761 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact
    26821 tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca
    26881 tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca
    26941 tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca
    27001 tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt
    27061 ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc
    27121 ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt
    27181 gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct
    27241 agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca
    27301 ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag
    27361 cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc
    27421 attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc
    27481 tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga
    27541 tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta
    27601 tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt
    27661 tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta
    27721 taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat
    27781 gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt
    27841 cctcctagca aatagattta tgattggtat attgttactt ataaatttat tctataggag
//
LOCUS       DAJSBC010000032        27020 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSBC010000032 DAJSBC010000000
VERSION     DAJSBC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.032"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..2305)
                     /locus_tag="ONT65_001466"
     CDS             complement(152..2305)
                     /locus_tag="ONT65_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR2329106.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="ONT65_001467"
     CDS             complement(2599..3447)
                     /locus_tag="ONT65_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2329107.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="ONT65_001468"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="ONT65_001468"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR2329108.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="ONT65_001469"
     CDS             complement(5948..6127)
                     /locus_tag="ONT65_001469"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR2329109.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="ONT65_001470"
     CDS             complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="ONT65_001470"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2329110.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /locus_tag="ONT65_001471"
     CDS             8275..10554
                     /locus_tag="ONT65_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2329111.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10647..11294)
                     /locus_tag="ONT65_001472"
     CDS             complement(10647..11294)
                     /locus_tag="ONT65_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329112.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11473..12192)
                     /locus_tag="ONT65_001473"
     CDS             complement(11473..12192)
                     /locus_tag="ONT65_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2329113.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12323..13474)
                     /locus_tag="ONT65_001474"
     CDS             complement(12323..13474)
                     /locus_tag="ONT65_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR2329114.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13691..14431)
                     /locus_tag="ONT65_001475"
     CDS             complement(13691..14431)
                     /locus_tag="ONT65_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2329115.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="ONT65_001476"
     CDS             complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="ONT65_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR2329116.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14815..15408)
                     /gene="yqeK"
                     /locus_tag="ONT65_001477"
     CDS             complement(14815..15408)
                     /gene="yqeK"
                     /locus_tag="ONT65_001477"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR2329117.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15392..16042)
                     /locus_tag="ONT65_001478"
     CDS             complement(15392..16042)
                     /locus_tag="ONT65_001478"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR2329118.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="ONT65_001479"
     CDS             complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="ONT65_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR2329119.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="ONT65_001480"
     CDS             complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="ONT65_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR2329120.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17485..18012)
                     /locus_tag="ONT65_001481"
     CDS             complement(17485..18012)
                     /locus_tag="ONT65_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR2329121.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18192..18977)
                     /locus_tag="ONT65_001482"
     CDS             complement(18192..18977)
                     /locus_tag="ONT65_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR2329122.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18970..19839)
                     /locus_tag="ONT65_001483"
     CDS             complement(18970..19839)
                     /locus_tag="ONT65_001483"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR2329123.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19840..21219)
                     /locus_tag="ONT65_001484"
     CDS             complement(19840..21219)
                     /locus_tag="ONT65_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR2329124.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="ONT65_001485"
     CDS             complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="ONT65_001485"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2329125.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21671..22147)
                     /gene="accB"
                     /locus_tag="ONT65_001486"
     CDS             complement(21671..22147)
                     /gene="accB"
                     /locus_tag="ONT65_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR2329126.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="ONT65_001487"
     CDS             complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="ONT65_001487"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR2329127.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="ONT65_001488"
     CDS             complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="ONT65_001488"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR2329128.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="ONT65_001489"
     CDS             complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="ONT65_001489"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2329129.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25177..25401)
                     /locus_tag="ONT65_001490"
     CDS             complement(25177..25401)
                     /locus_tag="ONT65_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2329130.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25454..26416)
                     /locus_tag="ONT65_001491"
     CDS             complement(25454..26416)
                     /locus_tag="ONT65_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR2329131.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26426..26866)
                     /locus_tag="ONT65_001492"
     CDS             complement(26426..26866)
                     /locus_tag="ONT65_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2329132.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7380 a         5995 c         4693 g         8952 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
    10681 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
    10741 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
    10801 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
    10861 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
    10921 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
    10981 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
    11041 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
    11101 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
    11161 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
    11221 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
    11281 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
    11341 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
    11401 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
    11461 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
    11521 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
    11581 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
    11641 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
    11701 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
    11761 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
    11821 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
    11881 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
    11941 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
    12001 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
    12061 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
    12121 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
    12181 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
    12241 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
    12301 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
    12361 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
    12421 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    12481 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    12541 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    12601 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    12661 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    12721 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    12781 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    12841 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    12901 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    12961 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    13021 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    13081 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    13141 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    13201 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    13261 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    13321 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    13381 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    13441 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    13501 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    13561 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    13621 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    13681 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    13741 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    13801 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    13861 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    13921 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    13981 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    14041 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    14101 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    14161 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    14221 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    14281 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    14341 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    14401 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    14461 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    14521 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    14581 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    14641 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    14701 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    14761 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    14821 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    14881 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    14941 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    15001 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    15061 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    15121 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    15181 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    15241 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    15301 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    15361 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    15421 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    15481 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    15541 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    15601 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    15661 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    15721 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    15781 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    15841 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    15901 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    15961 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    16021 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    16081 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    16141 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    16201 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    16261 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    16321 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    16381 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    16441 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    16501 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    16561 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    16621 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    16681 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    16741 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    16801 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    16861 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    16921 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    16981 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    17041 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    17101 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    17161 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    17221 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    17281 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    17341 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    17401 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    17461 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    17521 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    17581 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    17641 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    17701 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    17761 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    17821 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    17881 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    17941 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    18001 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    18061 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    18121 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    18181 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    18241 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    18301 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    18361 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    18421 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    18481 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    18541 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    18601 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    18661 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    18721 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    18781 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    18841 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    18901 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    18961 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    19021 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    19081 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    19141 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    19201 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    19261 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    19321 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    19381 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    19441 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    19501 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    19561 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    19621 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    19681 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    19741 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    19801 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    19861 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    19921 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    19981 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    20041 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    20101 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    20161 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    20221 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    20281 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    20341 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    20401 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    20461 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    20521 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    20581 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    20641 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    20701 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    20761 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    20821 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    20881 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    20941 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    21001 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    21061 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    21121 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    21181 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    21241 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    21301 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    21361 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    21421 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    21481 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    21541 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    21601 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    21661 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    21721 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    21781 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    21841 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    21901 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    21961 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    22021 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    22081 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    22141 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    22201 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    22261 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    22321 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    22381 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    22441 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    22501 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    22561 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    22621 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    22681 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    22741 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    22801 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    22861 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    22921 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    22981 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    23041 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    23101 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    23161 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    23221 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    23281 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    23341 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    23401 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    23461 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    23521 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    23581 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    23641 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    23701 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    23761 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    23821 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    23881 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    23941 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    24001 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    24061 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    24121 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    24181 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    24241 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    24301 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    24361 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    24421 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    24481 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    24541 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    24601 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    24661 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    24721 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    24781 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    24841 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    24901 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    24961 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    25021 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    25081 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    25141 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    25201 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    25261 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    25321 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    25381 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    25441 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    25501 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    25561 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    25621 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    25681 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    25741 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    25801 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    25861 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    25921 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    25981 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    26041 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    26101 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    26161 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    26221 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    26281 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    26341 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    26401 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    26461 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    26521 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    26581 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    26641 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    26701 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    26761 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    26821 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    26881 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    26941 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    27001 aacaactgat aaatcagcat
//
LOCUS       DAJSBC010000033        25576 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSBC010000033 DAJSBC010000000
VERSION     DAJSBC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.033"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            39..593
                     /locus_tag="ONT65_001493"
     CDS             39..593
                     /locus_tag="ONT65_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2329133.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(766..1239)
                     /locus_tag="ONT65_001494"
     CDS             complement(766..1239)
                     /locus_tag="ONT65_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2329134.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1251..1787
                     /locus_tag="ONT65_001495"
     CDS             1251..1787
                     /locus_tag="ONT65_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2329135.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1827..2198
                     /locus_tag="ONT65_001496"
     CDS             1827..2198
                     /locus_tag="ONT65_001496"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2329136.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2482..3753
                     /locus_tag="ONT65_001497"
     CDS             2482..3753
                     /locus_tag="ONT65_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2329137.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3998..5023
                     /locus_tag="ONT65_001498"
     CDS             3998..5023
                     /locus_tag="ONT65_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2329138.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5317..6426
                     /locus_tag="ONT65_001499"
     CDS             5317..6426
                     /locus_tag="ONT65_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2329139.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6509..7405
                     /locus_tag="ONT65_001500"
                     /pseudo
     CDS             6509..7405
                     /locus_tag="ONT65_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7421..8245
                     /locus_tag="ONT65_001501"
     CDS             7421..8245
                     /locus_tag="ONT65_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2329140.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8277..9587
                     /locus_tag="ONT65_001502"
     CDS             8277..9587
                     /locus_tag="ONT65_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR2329141.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9735..10994
                     /locus_tag="ONT65_001503"
     CDS             9735..10994
                     /locus_tag="ONT65_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2329142.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEYRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11004..11876
                     /locus_tag="ONT65_001504"
     CDS             11004..11876
                     /locus_tag="ONT65_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2329143.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11888..12718
                     /locus_tag="ONT65_001505"
     CDS             11888..12718
                     /locus_tag="ONT65_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2329144.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12758..14941
                     /locus_tag="ONT65_001506"
     CDS             12758..14941
                     /locus_tag="ONT65_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2329145.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15002..16222
                     /locus_tag="ONT65_001507"
     CDS             15002..16222
                     /locus_tag="ONT65_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR2329146.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16219..17679
                     /gene="gtfA"
                     /locus_tag="ONT65_001508"
     CDS             16219..17679
                     /gene="gtfA"
                     /locus_tag="ONT65_001508"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR2329147.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17756..18862
                     /locus_tag="ONT65_001509"
                     /pseudo
     CDS             17756..18862
                     /locus_tag="ONT65_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19184..19393
                     /locus_tag="ONT65_001510"
     CDS             19184..19393
                     /locus_tag="ONT65_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2329148.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19386..19535
                     /locus_tag="ONT65_001511"
     CDS             19386..19535
                     /locus_tag="ONT65_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR2329149.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19689..20354
                     /locus_tag="ONT65_001512"
     CDS             19689..20354
                     /locus_tag="ONT65_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2329150.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20638..21723)
                     /gene="xerS"
                     /locus_tag="ONT65_001513"
     CDS             complement(20638..21723)
                     /gene="xerS"
                     /locus_tag="ONT65_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR2329151.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22057..22290
                     /locus_tag="ONT65_001514"
     CDS             22057..22290
                     /locus_tag="ONT65_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR2329152.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22287..22694
                     /locus_tag="ONT65_001515"
     CDS             22287..22694
                     /locus_tag="ONT65_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2329153.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23831..24349)
                     /locus_tag="ONT65_001516"
     CDS             complement(23831..24349)
                     /locus_tag="ONT65_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR2329154.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25467..>25576
                     /locus_tag="ONT65_001517"
     CDS             25467..>25576
                     /locus_tag="ONT65_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2329155.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8709 a         3916 c         4779 g         8172 t
ORIGIN      
        1 atcattaact cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt
       61 taaggacaaa tgaagagata caagattttc agttttggct aagaagaaca agtaatccgg
      121 aaagagatag ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta
      181 taggattaaa agttggagat atacgaggca aaagtaagcc tattattgtt gaacataaaa
      241 caggtaagcg aaaaccgatt tttatagata atttgagaga agaaatttta ttatatactg
      301 aaggtaaaga agaaaatgat tggttatttc cgagtcggca acaaggtcga catataacaa
      361 gggatcgtgt ctatcagata tatgcagata tagcagaaaa attaggacga gatgatatag
      421 gaacacatac attaagaaaa acttttgggt atcactacta caagaaaact agagacattg
      481 ctaccttaat gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta
      541 taactgaaga tgaaatagga gcatcacttc gtggatttaa actaggtgta taatttaaaa
      601 atttttataa acagtaaaga caaagattaa aagctatttt tatctagaac gattttattt
      661 cataaagttc gcggaataaa agctggggtt actggtactt ggtataggtt cgtgttatat
      721 aaactaagta attaaataac atttttaggt tggaaaagga tgaaattaaa aaaagagaat
      781 caatacggtc tcaaaaacgc aaacgctgcg gaataagaaa aatactgata actcaacgtt
      841 tagcagacta tctaataggg atagtctgct tttttcgttt ttgaccatac ttttgacctt
      901 ctgcaaactt ccttgctttt cctctgtaga actcatgaaa tctgcaaaac gaattgccgt
      961 ttcctctgtc tgctttttcg cgatatgggt gtatatatca tccatagtgg ttttaatatc
     1021 tgtatgtcca agtcgaacat acacttcttt gacagacgca ccagattcaa agagcagact
     1081 acaatgggta tgacgaaaac catgaagtgt gatatgtttt aagttattct ctttaatgat
     1141 gattttcaaa ttgtgattta catagtctaa ataaaggggc ttattttctt ctgatgaaaa
     1201 caaaagttgt tcaccatctt ttataggttt gattctcatt tttagatatt atgcccgcac
     1261 gtattcatca catttctatt gtgaatcgct ttattcgacc gacatttgac ttttatcata
     1321 atatactcgg tttaaaatta ctaatgaaaa caattaatca agacgatcat accatgtatc
     1381 acctgttttt ttcagataat caccaccgtg tgggaactga actgactttt tttgaagtac
     1441 aagaagggaa caaccagttt tttggaacca atacgattga acgaacgata ctaaaagttc
     1501 cttccgaagc atcattgcat ttttgggaaa tatattttga aacacaaggc gtttgccatt
     1561 atggaatcga atcattttct ggacgaccta tcttacgttt tgaagggcca gatgcaacac
     1621 gattagcttt agttccctta cgcgaatttg aagatattga tgattatttc ccgtatgtca
     1681 cagaccagat ccctacagag catgccattt taggaattga tgccattcaa ctacgcgtac
     1741 aatattccga tgcgactgaa cgagagttat tgtcggtagg ctggtagcac aaagaatacg
     1801 tcagtttctt ttcgggtcat caacgagtga caatcttaga aaatcaagac actcagttct
     1861 atcaagaagt tcagattatt caagatcgtg agaatccagt tgccattgaa gggattggta
     1921 gcgtccatca tgttgcgttt ggcgtagaaa ataaatctga tttacaacgc atcgacaaac
     1981 aattacaaga gcgcaatttt ataaattcag gaataaaaga tcgtgaattt tttgtatcac
     2041 tttattatcg tgacccaaat caattattga ttgaaattgc tacaggggaa ggaaacctag
     2101 atgcaaaagc gtatgaaaat caaagtccac gctttgaaga aattccttta tttttaccgc
     2161 agtacttaaa tttcattcgt gaacaagtgg agcaataatt agctaatcaa atccatgacg
     2221 aagagtaaga ggttgactca taagtcaaaa aataaagaaa aacgcatcaa atccattcta
     2281 aagaatgttg atttgatgcg gttcctattt ttataaacta tcattttcga ctaataaaac
     2341 tacttatgag tcagcctctc tttttttctt gatttttata cctctttcta ggtaaactaa
     2401 acatctgcca aaaataccgt gtctcattga aacattctca actatttcaa aataaagggg
     2461 agtaccaaat gaacgagtat attgaaatta tccacgcaag agaaacaact taaaaatgtc
     2521 agtctcaaaa ttcccaaaaa taaaattagt atttttacca gcgtatctgg ttcaggaaaa
     2581 tcatcgattg tttttgatac tattacccaa gaagctggtc gtcaactgaa tgacacctat
     2641 gatagtttta cccgtttatt tttacctaaa tacagtcgtc cagaggttga tgagattgat
     2701 aatctttcta cggcgattgt ggtcaatcaa aagaagattg gtggcagtgc gcgctcaacg
     2761 ttagggacga ttactaacat caatgcgtta tttcgtgtgt tgttttcatg ttttgcaaca
     2821 cctagtttag gatatgcaaa tgcttattca tttaatgatc ctagtggaat gtgttctgtc
     2881 tgtcagggaa tcggtaagac gattgcattt ttaataggat tgctttattt ttcggctatt
     2941 gataatgctg attttgtatt ttctgatcca tttaccgcag aagttttgtc tggaattgag
     3001 gaacaggcaa agaatcatgg ttattttgta ttggtgcata atgttaattc agcagaagat
     3061 gttaaaacta ttcagagaaa ttggcgtttt gaggggttca ttgttgttgg tgtacgtgta
     3121 aaagaatttt tggaattaaa taacgttttt gatggtcctg ttgtttatat tgatacgcat
     3181 ctttcaaaag agacattaag agaacttgaa caacaaatga atcgtttatt tgtaaataca
     3241 aatgattacc aagctagtac attggcagtg gaacatttga ttcattatgg tcatgagaaa
     3301 atagcgtttc tttcttttga ctacattgta ggtgaaccaa gtgttattca agaacgacat
     3361 caagcttata tcgatacatt aaagaaatat catttaacct ttaaatcgca ttggatatat
     3421 aacaactctg aatttgagcg aatttatcaa gaattagctg aatttactgc aattgttgtt
     3481 actggagatt atttagcggc taaattagta aaatttttaa aaaataagaa taaacgagtg
     3541 atggaacaat tatcgattat tggatttgat gatattactt ttgcagaatt aatggatcct
     3601 gcgttgacaa caatacgact aaatccaggt aacaaggggg cgattgcttt tcaccaactg
     3661 attggactgg ccaaacaaga agaaataaaa aatcaagtac tactattaga gggagaattg
     3721 atttctcgag actctgttca caaaatcaaa taaaaaagct ctgtgaaata agtatagtta
     3781 acaataaaat atccgaacga tagagagaat gaactgtgat ctagcagttc ctcgtcgcaa
     3841 attcactaga attagcatta tttactctaa attgttcgga tattttttct tgtttttaat
     3901 tatgattaca gtgcctattt ttaacgaaat ttgcttattt tcaaaatgta tgattatatg
     3961 ttagtaaaca ctttcaaata tgaaaagagg agctattatg gtaggaatta gggatgttgc
     4021 aaaacgggcg ggtgtgtcca ttgcaacagt atcgcgcgtg ctaaatgagg atccgacact
     4081 ttcagtaacc gatgaaacca aacgaaagat tgcagaatcg gttaattttt atcattatgt
     4141 caaaaaaagt cccgttgcaa aaaatttaac aaatattgca ttaataacga cagtttctga
     4201 agtagatgag ttggaagatc cctattttcg aacgattcgc agagggattc aagtcgaagc
     4261 agaacaaagc aaagtcaatt tgaagaaaat tattcgtttg tcagaaacac cgttggagca
     4321 agacgatttg ctaaaatgtc aaggtgtgtt gattattggg caggtgctat cttctgtgat
     4381 tgaagaagta tcagcaatta attcgaatgt cgtagtgatt gatgacccta gtattgaagc
     4441 tgaagtagat gcagtttata ctgatttaac aaaagcaacg aatcagcatt tagatcgatt
     4501 gtataaaaaa ggacaccgaa acattgcgtt tatcggagga ggacgtgttc gtttagatag
     4561 ccaaggaaat aaacacgtgt cagatgatga ttatcgaaaa atagcctatg agacgtggat
     4621 gaaacaaaaa ggattagaaa aatatagtca aatttattta gaaggctgga ccacacttga
     4681 cggaatgcag gcagcagatt tacttttgaa aaaacatgct aaagataagt tgccgactgc
     4741 tatcatggta ggaaatgatc ctattgctgt gggggtttat cgttcacttc aaaaaaatgg
     4801 tctacatatt ccagaagata tttcgattgt gagttttgat aatattgaag ttgctgaatt
     4861 tttaacacct gcattaaata cggtgaatgt gaatacagaa gaaattggaa aactagcagt
     4921 tcgcatggtt aatgaacgaa taaaagaagt tagaaatgtt tcgatcaaag tgattgtttc
     4981 taataatatt attgttagag aaagtgaaaa aaatatcgat taaaaaagta aacatccaaa
     5041 aaagggtgtt tacttttttg tttactaata tttttagtaa aaaatctcga caaaacatta
     5101 agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag
     5161 atgaaacatt tcaaaaaaat agctttaaca gggatagctg ttttaactgg gacattactc
     5221 gcagcatgtg gtgggggaga tagtaattct aaagacgggc aagtaacttt aagctatgcg
     5281 agttgagata ctgaccaagc tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa
     5341 aatcctgata tcaaagtaga aatggagaca acgccttggg atcaatattg ggttaaatta
     5401 gaagctgcaa caacgggagg taatatgcca gatgtagtaa ccatgcactc tagcgaatcc
     5461 tataaatata tgtcacagaa tgctttgatg ggacttgatg atgttatcaa agaaaatgat
     5521 attgacatgg gaaactttac tgaaggaatt gctgatttct atacgcttga agacgaacta
     5581 tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt acaacaaaga attatttgat
     5641 aatgcaggtg tcgattatcc agatgaaaca tggacatggg aaacactcag acaagctgca
     5701 attgatttaa cagatgaatc aaaaggcatc tatggttttg catcacatat tggaacagag
     5761 actggctact ggccatttat ttatcaaaat gggggagaag tatttgccga tgacaataca
     5821 aaatcaggtt tgaatacccc agaagcacaa gaagctttag ctttttatcg agatattgca
     5881 cttgttgatc atgcttcacc aacaattaca gaattacaag atcctgataa gttaagtcgt
     5941 tttcaatcag gtagattagc aatgatgatt gaaggaaact ggtttgcacc gggatttttg
     6001 aaaaatgact atctagttga aaatgttgct tctgccgtgt tacctaaggg aaaagttaat
     6061 gcaacaatta caaatggact tggttgggct gccagtgcga gtacaaaata cccagaagaa
     6121 gtgaaaaaac taatggcatt tttagctagt gaggacgcca ataaaatcca agcggaaacg
     6181 ggggcatcaa tcccagcact aaaaggcttt gaagacgagt gggctgcttc ttcagataca
     6241 attgatttga gtgcttttgg aaaaatgttg gagtatggag tccctcgtcc atacaataaa
     6301 gatggattac gagctgaaca agcagaagtt caaattatta accgtattct gtctggtgaa
     6361 gtaactgttg aagatggaac cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga
     6421 aattaaatta gttaattata caatatacag gcgaaaggat tctatttcgt ctgtatatta
     6481 atggatgaat accaaaaagg gggatattat gaaaaatcga atttttggaa aatatcgtaa
     6541 agatcaagtg atatggggct atatttttat ttttccaacg gcattaggat taattttatt
     6601 aaatatatgg ccagcaattc aaacagcaat tttaagtttt caaaaaacgg tagggttcgg
     6661 tgatactgaa tgggtcggat taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt
     6721 tcagtcttta ttaaacacat taatttatgc agtggtttcg atacctagta ttgttgtatt
     6781 atccttgttg gtggcagttt taatgaatag aaaagttaaa ggattgtcga tttatcggac
     6841 aatttatttt ctaccagtcg ttgctgcacc atccgcagtc gcaatgattt ggcgctggat
     6901 gttcaataat gattatggac tgattaataa catgttggct aaaataggtc tcgatggacc
     6961 agcatggtta acggatccat cgattgcgat ttattctatt atcattgtag gagtttggag
     7021 tgctattggt tataacatgg tgttattact agccggctta taagaaattc ctaaagatta
     7081 ttatgaagca gcggaaattg atggtgctag tccaatccag cagtttttca acattacgtt
     7141 accattagtt acaccaacat tattttttgt agttgtaaca acagttatca atgctttcca
     7201 agtatttgat gttattttta tgatgattac tcctaatagt accgccatgt ttaaatcaca
     7261 atcattagcc tatttattct ataaacattc atttatttta aatgacaagg gatatggttc
     7321 ggcaatcgtt atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt
     7381 gcagaaaaag tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc
     7441 aaatgcgcca atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat
     7501 ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat
     7561 tttccctgag aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat
     7621 ggctttttat atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt
     7681 ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt
     7741 cagcttagta cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt
     7801 attagcacaa aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt
     7861 tagtgcgttt ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga
     7921 ggaagcggcc tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc
     7981 gcttgctaaa tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga
     8041 tttaatgtgg ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt
     8101 agcaaactta caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt
     8161 agcaatttgg ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat
     8221 tgctacaaca ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga
     8281 caaaaattac atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg
     8341 cgatgttaac tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac
     8401 gactaaaaga atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg
     8461 ctgatattgt ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca
     8521 atgctattca agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa
     8581 aatatgggtt gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc
     8641 tacggacact accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg
     8701 cttggttatt gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga
     8761 cgagtattaa gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg
     8821 agcatttagg aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca
     8881 ttaatcatat ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa
     8941 ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt
     9001 tgaaacgttt tggttactat attacagaat caagtgaaca caatgcagaa taccatccat
     9061 actttattaa atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat
     9121 acttacgtcg ttgtgaacaa caaattaatg attgggaaag catgcgagag gacattgtaa
     9181 ataatgggga gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta
     9241 ttacgacagg aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa
     9301 acttaccgac agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac
     9361 caacctatgt cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg
     9421 ttcaagaact gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag
     9481 cgtatttaga tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg
     9541 atgatttaat cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa
     9601 gagtaaggaa ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca
     9661 aaacacttag taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata
     9721 aggaggagca taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag
     9781 caacgatctc tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa
     9841 tagagttttt taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt
     9901 ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag
     9961 taattaaaac acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa
    10021 gtatagaatt gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt
    10081 atttagagaa tatcaaaaat ggatatgcac aacgttttgc tattgagtat cgtgtatata
    10141 gcataccatt aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa
    10201 tggggattca agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag
    10261 atcaaaacaa agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc
    10321 accaactagc tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt
    10381 tctcagaagt taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat
    10441 tagatttgtt aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg
    10501 atacagtagt tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa
    10561 tgccgatgat tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag
    10621 atgaagcagg tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa
    10681 cttctcaaca taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga
    10741 tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata
    10801 ctcaggattc accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt
    10861 ggttagccaa agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac
    10921 acaatggcaa tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag
    10981 ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa
    11041 tcatttttgt agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc
    11101 aaggtgcttt ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg
    11161 gtttgaacaa ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca
    11221 ctttaattgt gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc
    11281 gggcgttgaa cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag
    11341 cggtcttatc gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac
    11401 caaaaattgg tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat
    11461 ggggagcgat ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca
    11521 tcattttctt agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg
    11581 atggggcgaa tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta
    11641 tttcaatggt atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg
    11701 ctttaactgg tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt
    11761 atgcttttaa aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct
    11821 taattgggat tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg
    11881 tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg
    11941 gggcttcatg attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc
    12001 cgatattatg aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag
    12061 attgttagcg gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc
    12121 agtagttgct gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac
    12181 tcgtgggaaa gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt
    12241 ccaagtaatt atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg
    12301 gggattaatc ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg
    12361 ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag
    12421 tcactttaca acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac
    12481 tattattctt aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa
    12541 tcgtgattct aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt
    12601 taacgactat ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt
    12661 ttatcttgct ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc
    12721 aataaattat tctcattata aaggagttca taaacttatg attatatttg acaatcacac
    12781 acaaacattt catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa
    12841 ttatttaacg catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta
    12901 cccccgaaca gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc
    12961 tttagacaca ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc
    13021 gcataatatt aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga
    13081 aattcaaaaa ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga
    13141 tgaagctgaa acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt
    13201 gttctacaca atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa
    13261 tagtgatgaa acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg
    13321 tccactcgag ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga
    13381 aaaaattcat acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca
    13441 aaatccgttt gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg
    13501 atttaattta gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca
    13561 aacacgggtc attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga
    13621 ctcatttcaa acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc
    13681 acagacgtat catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga
    13741 acgtcccacg ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat
    13801 catgaatatt gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg
    13861 ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg
    13921 caaacttgct tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt
    13981 tgggatttgg tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc
    14041 agattgggca ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct
    14101 tgatttttca agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga
    14161 tcgtgtaccg attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc
    14221 tgtcttgttg gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata
    14281 tgaattttta gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg
    14341 tggaggcggt cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga
    14401 caataccgat gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat
    14461 ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag
    14521 tttaactatt cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac
    14581 gcaattaact tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca
    14641 tcgccaactt ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga
    14701 tactgcatgg atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt
    14761 attagcagaa gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc
    14821 aacttatcaa atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta
    14881 tattgactca gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata
    14941 gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa
    15001 tatgttttat ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga
    15061 agtacgtgaa gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt
    15121 tagaatcgat gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga
    15181 tggagaagat gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt
    15241 gtggttaaaa gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga
    15301 agccgatgtt cctaatgaac gtttagatga atatattggt gaagatggct attattcgat
    15361 ggtttttgat tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga
    15421 tagccaatgg acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca
    15481 agataaaggt tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa
    15541 atatattcca gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat
    15601 gttactaaga ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga
    15661 aatggcatca ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt
    15721 agaatatgga ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa
    15781 ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa
    15841 tgtatggtta aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa
    15901 tcaacagtcg gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata
    15961 caaagacgtg ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt
    16021 gctttatgaa cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac
    16081 agaacaagaa tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga
    16141 tgttttatta gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc
    16201 taattatgga ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat
    16261 agtttaggaa aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt
    16321 attggtggtg tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg
    16381 agtgattata cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt
    16441 gaagattatt atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt
    16501 ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt
    16561 catgaatttt tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt
    16621 aaagacaaag cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg
    16681 aatacctttg gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt
    16741 attcgagaaa ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg
    16801 tttgcttatg cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg
    16861 gaacttttag acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa
    16921 atccatgagc attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac
    16981 ttcgccttgc caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc
    17041 aattggttga aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt
    17101 ggtgttgtgg acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa
    17161 ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta
    17221 gatatttatc aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat
    17281 ctgttagcac gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg
    17341 ttattagcag gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt
    17401 aatcgtcatt actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa
    17461 caattatttg cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg
    17521 attgaagtag caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt
    17581 agtgatagtg caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa
    17641 gatataaaaa ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa
    17701 tcctttatac tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga
    17761 taagtataaa acaaatacag catttaataa atggttttct gccataaaat taggaaaact
    17821 ttctgtcaac tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt
    17881 tgaagctgtg ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga
    17941 tttgagtaca gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag
    18001 tcatacccaa ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt
    18061 tcaacaactg ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta
    18121 tctgattgat tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg
    18181 tcaaacaaaa tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat
    18241 gtatccaact gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt
    18301 attggtgaat caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa
    18361 gatggatcag atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa
    18421 agttcacgtg atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct
    18481 ggttcgttta ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga
    18541 taagaacgga cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat
    18601 ctctgatatg tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca
    18661 catgaccatt aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt
    18721 aacgatgatt tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc
    18781 agtgttccaa atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct
    18841 agaaaaatta attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt
    18901 tgaataacag ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt
    18961 tgacttattt tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag
    19021 tgatattttt tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata
    19081 caaagattta taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta
    19141 gtagtgttct tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa
    19201 aaatgaatta atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc
    19261 aaaagcaatt atggtttcaa aaagctatac ctattacgaa aatagaagat taggactagt
    19321 acctgttgaa tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca
    19381 gaatcatggt taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg
    19441 gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa
    19501 agaagaaaaa aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct
    19561 caaagtacta tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg
    19621 aaagtagaag aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt
    19681 tctttaaaat ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt
    19741 tattgagcaa atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat
    19801 tagatcgagc agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg
    19861 tgaagaaaag gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt
    19921 caacttttta tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt
    19981 ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg
    20041 aaaataaaaa cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt
    20101 ttgtagaacc cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta
    20161 cctttttaaa agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt
    20221 cttataatgg aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa
    20281 cgatcgttgg tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc
    20341 ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac
    20401 aaacgacttt aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg
    20461 atcccacaga ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata
    20521 ataaaaaata aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact
    20581 ataatacagg cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca
    20641 taaagcattt aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt
    20701 attggtactt gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta
    20761 gagtctagta gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt
    20821 tgaatatttt gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg
    20881 gtaaattgct agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat
    20941 agctagataa ttagctaaat attccattgc gaatgctgct atattgacag catctctttt
    21001 atttcctttt ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat
    21061 atttataacc tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc
    21121 tctttctaca tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc
    21181 attggcaata taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat
    21241 ggcattggca cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg
    21301 attattgtct aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa
    21361 tagaaccttt aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt
    21421 attatctttg agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt
    21481 caaatgagat ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga
    21541 agtatcagga acgatagact cactcagaag ccattcataa aagagcttat aattttttag
    21601 atattctaaa gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt
    21661 tatataatcg ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt
    21721 catgtcattc atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc
    21781 aattatagaa tgttttgtca attggtatgc tctaaaattt attaatttgt ttcaaaaaaa
    21841 gcatataaac aggtatacgt ttaataattt aaaataaatg gtatacataa atagggaagg
    21901 aaatagaagg gagcaatcta aattttttga attttcttaa ttaattatat agaaagaaca
    21961 atctttctat agagttagac atgagaggga ctatgcttga tttaaaatca acaagcgtat
    22021 atactgtata tacaaagaaa aaggagggag aagactatgg caattaaaga aagaaaattt
    22081 agggaagtgg ggaactcagt agtattaact atttcaaagg agtttcttaa agaaaatggg
    22141 ttaaatcccg gagatacggt ttttttagat gaagaaaaac ttcgagcggc aattacaaaa
    22201 aaacaagcct tatctgatga aattgattta gcaatatctc aagcattaac tgaatatgat
    22261 actactttta gagagcttgt cgataaatga gtattcatta cctagaagcc aaagatatta
    22321 taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa tgaacccctt ggtgtaaagg
    22381 atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc ctcagctttt ggtgaagagg
    22441 tttatccttc gatttatgaa aaagcagcta tcctacttat acaattgata aagaaacatc
    22501 cttttcacaa tgcaaataaa cgtactgctt tcttagctac ttttgtcttt ttgaaaatca
    22561 atggacagct tttagctatt gatcaaaaag atgctgtaaa tttagcggta tatattgcta
    22621 cgtatgataa ggatttcgat caactaaaat atgaagtgat caacactata aagacacata
    22681 cgcagcccat ttaaggagtt actgtatgtg tctttatagg gttattagct gctatagcga
    22741 aataattgaa gtaaattaca aatagctata attcaaatcc aactatatag aaaacgtaaa
    22801 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
    22861 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
    22921 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
    22981 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
    23041 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
    23101 ttatgaaatg aaagaccttt tacatcttgc agaaatcggt actgggatta aactaagcaa
    23161 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
    23221 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
    23281 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
    23341 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
    23401 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
    23461 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
    23521 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
    23581 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
    23641 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
    23701 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
    23761 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
    23821 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
    23881 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
    23941 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
    24001 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
    24061 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
    24121 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
    24181 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
    24241 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
    24301 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
    24361 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
    24421 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
    24481 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
    24541 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
    24601 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
    24661 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
    24721 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
    24781 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
    24841 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
    24901 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
    24961 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
    25021 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
    25081 ccgcgaattt actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat
    25141 taagggatca agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta
    25201 aacaattata gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac
    25261 tttcttattc tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt
    25321 acgagtacta ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc
    25381 cccacttagc ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag
    25441 aagaaaacat accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag
    25501 aattcaagca acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc
    25561 gagaatatga actaac
//
LOCUS       DAJSBC010000034        25425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSBC010000034 DAJSBC010000000
VERSION     DAJSBC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.034"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..84
                     /locus_tag="ONT65_001518"
     tRNA            12..84
                     /locus_tag="ONT65_001518"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ONT65_001519"
     tRNA            88..160
                     /locus_tag="ONT65_001519"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ONT65_001520"
     tRNA            168..249
                     /locus_tag="ONT65_001520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ONT65_001521"
     tRNA            267..339
                     /locus_tag="ONT65_001521"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ONT65_001522"
     tRNA            351..422
                     /locus_tag="ONT65_001522"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ONT65_001523"
     tRNA            439..524
                     /locus_tag="ONT65_001523"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ONT65_001524"
     tRNA            535..608
                     /locus_tag="ONT65_001524"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ONT65_001525"
     tRNA            618..691
                     /locus_tag="ONT65_001525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ONT65_001526"
     tRNA            711..783
                     /locus_tag="ONT65_001526"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ONT65_001527"
     tRNA            811..884
                     /locus_tag="ONT65_001527"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ONT65_001528"
     tRNA            898..971
                     /locus_tag="ONT65_001528"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ONT65_001529"
     tRNA            995..1084
                     /locus_tag="ONT65_001529"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ONT65_001530"
     tRNA            1097..1170
                     /locus_tag="ONT65_001530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ONT65_001531"
     tRNA            1189..1264
                     /locus_tag="ONT65_001531"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ONT65_001532"
     tRNA            1266..1336
                     /locus_tag="ONT65_001532"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ONT65_001533"
     tRNA            1350..1423
                     /locus_tag="ONT65_001533"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ONT65_001534"
     tRNA            1448..1535
                     /locus_tag="ONT65_001534"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ONT65_001535"
     tRNA            1557..1628
                     /locus_tag="ONT65_001535"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ONT65_001536"
     tRNA            1651..1740
                     /locus_tag="ONT65_001536"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ONT65_001537"
     tRNA            1753..1826
                     /locus_tag="ONT65_001537"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ONT65_001538"
     tRNA            1845..1917
                     /locus_tag="ONT65_001538"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ONT65_001539"
     tRNA            1921..1993
                     /locus_tag="ONT65_001539"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ONT65_001540"
     tRNA            1998..2068
                     /locus_tag="ONT65_001540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ONT65_001541"
     tRNA            2081..2154
                     /locus_tag="ONT65_001541"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ONT65_001542"
     tRNA            2166..2237
                     /locus_tag="ONT65_001542"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="ONT65_001543"
     CDS             2503..3696
                     /locus_tag="ONT65_001543"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR2329156.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ONT65_001544"
     CDS             3826..5298
                     /locus_tag="ONT65_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329157.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ONT65_001545"
     CDS             5381..6169
                     /locus_tag="ONT65_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR2329158.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ONT65_001546"
     CDS             complement(6236..6904)
                     /locus_tag="ONT65_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR2329159.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ONT65_001547"
     CDS             7019..8731
                     /locus_tag="ONT65_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329160.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ONT65_001548"
     CDS             8734..10503
                     /locus_tag="ONT65_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329161.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ONT65_001549"
     CDS             complement(10708..11274)
                     /locus_tag="ONT65_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2329162.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ONT65_001550"
     CDS             complement(11274..12230)
                     /locus_tag="ONT65_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR2329163.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ONT65_001551"
     CDS             complement(12230..12886)
                     /locus_tag="ONT65_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR2329164.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ONT65_001552"
     CDS             13256..15274
                     /locus_tag="ONT65_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2329165.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="ONT65_001553"
     CDS             15701..18115
                     /locus_tag="ONT65_001553"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR2329166.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="ONT65_001554"
     CDS             18463..20016
                     /locus_tag="ONT65_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2329167.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="ONT65_001555"
     CDS             complement(20397..22181)
                     /locus_tag="ONT65_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329168.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="ONT65_001556"
     CDS             complement(22196..23917)
                     /locus_tag="ONT65_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329169.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="ONT65_001557"
     CDS             24017..24778
                     /locus_tag="ONT65_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2329170.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7873 a         4656 c         5391 g         7505 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    25261 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    25321 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    25381 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtt
//
LOCUS       DAJSBC010000035        23831 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSBC010000035 DAJSBC010000000
VERSION     DAJSBC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.035"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(55..789)
                     /locus_tag="ONT65_001558"
     CDS             complement(55..789)
                     /locus_tag="ONT65_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2329171.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ONT65_001559"
     CDS             1116..1526
                     /locus_tag="ONT65_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR2329172.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ONT65_001560"
     CDS             1519..2220
                     /locus_tag="ONT65_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR2329173.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ONT65_001561"
     CDS             2220..3806
                     /locus_tag="ONT65_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329174.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ONT65_001562"
     CDS             3825..4046
                     /locus_tag="ONT65_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR2329175.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ONT65_001563"
     CDS             4043..4804
                     /locus_tag="ONT65_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR2329176.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ONT65_001564"
     CDS             5184..5693
                     /locus_tag="ONT65_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2329177.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ONT65_001565"
     CDS             complement(5768..6307)
                     /locus_tag="ONT65_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR2329178.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT65_001566"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ONT65_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR2329179.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ONT65_001567"
     CDS             7320..8171
                     /locus_tag="ONT65_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR2329180.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ONT65_001568"
     CDS             8199..8834
                     /locus_tag="ONT65_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR2329181.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ONT65_001569"
     CDS             8854..9627
                     /locus_tag="ONT65_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2329182.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ONT65_001570"
     CDS             10035..10832
                     /locus_tag="ONT65_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR2329183.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ONT65_001571"
     CDS             10810..11223
                     /locus_tag="ONT65_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2329184.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ONT65_001572"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ONT65_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2329185.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ONT65_001573"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ONT65_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR2329186.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="ONT65_001574"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="ONT65_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR2329187.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT65_001575"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ONT65_001575"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR2329188.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ONT65_001576"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ONT65_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR2329189.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ONT65_001577"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ONT65_001577"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR2329190.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT65_001578"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ONT65_001578"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR2329191.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT65_001579"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ONT65_001579"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR2329192.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ONT65_001580"
     CDS             21907..22485
                     /locus_tag="ONT65_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2329193.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ONT65_001581"
     CDS             22537..23019
                     /locus_tag="ONT65_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR2329194.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ONT65_001582"
     CDS             23136..23510
                     /locus_tag="ONT65_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329195.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAJSBC010000036        23261 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSBC010000036 DAJSBC010000000
VERSION     DAJSBC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.036"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT65_001583"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT65_001583"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR2329196.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ONT65_001584"
     CDS             complement(1172..1867)
                     /locus_tag="ONT65_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR2329197.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ONT65_001585"
     CDS             complement(1880..2596)
                     /locus_tag="ONT65_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR2329198.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ONT65_001586"
     CDS             2926..3534
                     /locus_tag="ONT65_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR2329199.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ONT65_001587"
     CDS             3859..4182
                     /locus_tag="ONT65_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR2329200.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ONT65_001588"
     CDS             4364..5734
                     /locus_tag="ONT65_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR2329201.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ONT65_001589"
     CDS             complement(5864..7165)
                     /locus_tag="ONT65_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2329202.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="ONT65_001590"
     CDS             complement(7519..9477)
                     /locus_tag="ONT65_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR2329203.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ONT65_001591"
     CDS             complement(9654..9920)
                     /locus_tag="ONT65_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2329204.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT65_001592"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT65_001592"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR2329205.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT65_001593"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT65_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR2329206.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="ONT65_001594"
     CDS             13264..13827
                     /locus_tag="ONT65_001594"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2329207.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ONT65_001595"
     CDS             complement(14080..14601)
                     /locus_tag="ONT65_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR2329208.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ONT65_001596"
     CDS             complement(14604..15446)
                     /locus_tag="ONT65_001596"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR2329209.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ONT65_001597"
     CDS             complement(15439..16164)
                     /locus_tag="ONT65_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR2329210.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ONT65_001598"
     CDS             16340..16636
                     /locus_tag="ONT65_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329211.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="ONT65_001599"
     CDS             17204..18796
                     /locus_tag="ONT65_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2329212.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ONT65_001600"
     CDS             18809..19549
                     /locus_tag="ONT65_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2329213.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ONT65_001601"
     CDS             19717..21066
                     /locus_tag="ONT65_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2329214.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ONT65_001602"
     CDS             complement(21194..21784)
                     /locus_tag="ONT65_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2329215.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="ONT65_001603"
     CDS             22020..23099
                     /locus_tag="ONT65_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2329216.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4820 c         4527 g         7253 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DAJSBC010000037        23077 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSBC010000037 DAJSBC010000000
VERSION     DAJSBC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.037"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            102..2543
                     /locus_tag="ONT65_001604"
     CDS             102..2543
                     /locus_tag="ONT65_001604"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR2329217.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ONT65_001605"
     CDS             complement(2873..3577)
                     /locus_tag="ONT65_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329218.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ONT65_001606"
     CDS             complement(3764..4009)
                     /locus_tag="ONT65_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329219.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ONT65_001607"
     CDS             complement(4026..4877)
                     /locus_tag="ONT65_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2329220.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ONT65_001608"
     CDS             5043..6047
                     /locus_tag="ONT65_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR2329221.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="ONT65_001609"
     CDS             complement(6288..6644)
                     /locus_tag="ONT65_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2329222.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="ONT65_001610"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="ONT65_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2329223.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ONT65_001611"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ONT65_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2329224.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="ONT65_001612"
     CDS             complement(11004..11765)
                     /locus_tag="ONT65_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2329225.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="ONT65_001613"
     CDS             12043..12564
                     /locus_tag="ONT65_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR2329226.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="ONT65_001614"
     CDS             12623..13483
                     /locus_tag="ONT65_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR2329227.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="ONT65_001615"
     CDS             13668..15983
                     /locus_tag="ONT65_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2329228.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="ONT65_001616"
     CDS             16165..18006
                     /locus_tag="ONT65_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR2329229.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="ONT65_001617"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="ONT65_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR2329230.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19359..21458
                     /locus_tag="ONT65_001618"
     CDS             19359..21458
                     /locus_tag="ONT65_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2329231.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21654..22517
                     /locus_tag="ONT65_001619"
     CDS             21654..22517
                     /locus_tag="ONT65_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2329232.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22622..>23077
                     /locus_tag="ONT65_001620"
     CDS             22622..>23077
                     /locus_tag="ONT65_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2329233.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         7771 a         4038 c         4810 g         6458 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aagcttgaga ctacgcagtc tcaagctttt ttttctatag gaaggaaatt cattcattat
    19261 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
    19321 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
    19381 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
    19441 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
    19501 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
    19561 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
    19621 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
    19681 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
    19741 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
    19801 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
    19861 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
    19921 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
    19981 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
    20041 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
    20101 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
    20161 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
    20221 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
    20281 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
    20341 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
    20401 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
    20461 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
    20521 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
    20581 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
    20641 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
    20701 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
    20761 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
    20821 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
    20881 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
    20941 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
    21001 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
    21061 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
    21121 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
    21181 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
    21241 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
    21301 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
    21361 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
    21421 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
    21481 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
    21541 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
    21601 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
    21661 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
    21721 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
    21781 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
    21841 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
    21901 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
    21961 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
    22021 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
    22081 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
    22141 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
    22201 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
    22261 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
    22321 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
    22381 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
    22441 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
    22501 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagcttat aataatttta
    22561 attaacatag tgttatatct ttttttattt gtaattatta taaattgttg gggttgagta
    22621 aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg
    22681 gtttaagctg acaccgcaaa gagaagctac tctgcttgtg cttttggaaa atgagaaaga
    22741 gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa aagagtccgg aaattggact
    22801 tgctactgtt tatcgaaccc ttgaaatctt gacagaattg aaaattgtcg ataaagtaag
    22861 ttttaatgat ggattagccc gttatgattt gagaaaagaa ggcgccaaac atttccatca
    22921 tcatttacta tgcttgaact gcggcacgat cgaggaagta gaagaggatc ttttacttga
    22981 agtggagcaa gtagtagaag aacgttatca ttttttagta aaagatcatc ggctaacgtt
    23041 ccatggaatc tgtcaggaat gctatgaaaa agaaaaa
//
LOCUS       DAJSBC010000038        23067 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSBC010000038 DAJSBC010000000
VERSION     DAJSBC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.038"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(111..566)
                     /locus_tag="ONT65_001621"
     CDS             complement(111..566)
                     /locus_tag="ONT65_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329234.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ONT65_001622"
     CDS             complement(720..1928)
                     /locus_tag="ONT65_001622"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR2329235.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ONT65_001623"
     CDS             complement(2040..2372)
                     /locus_tag="ONT65_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2329236.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ONT65_001624"
     CDS             2520..3395
                     /locus_tag="ONT65_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2329237.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ONT65_001625"
     CDS             complement(3477..4058)
                     /locus_tag="ONT65_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR2329238.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ONT65_001626"
     CDS             complement(4064..5323)
                     /locus_tag="ONT65_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2329239.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ONT65_001627"
     CDS             complement(5325..6371)
                     /locus_tag="ONT65_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329240.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ONT65_001628"
     CDS             complement(6606..6881)
                     /locus_tag="ONT65_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR2329241.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ONT65_001629"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ONT65_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR2329242.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ONT65_001630"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ONT65_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR2329243.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="ONT65_001631"
     CDS             complement(9980..10660)
                     /locus_tag="ONT65_001631"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR2329244.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ONT65_001632"
     CDS             complement(10709..11344)
                     /locus_tag="ONT65_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2329245.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ONT65_001633"
     CDS             complement(11410..12813)
                     /locus_tag="ONT65_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR2329246.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ONT65_001634"
     CDS             complement(12800..13831)
                     /locus_tag="ONT65_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329247.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ONT65_001635"
     CDS             13862..14083
                     /locus_tag="ONT65_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR2329248.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ONT65_001636"
     CDS             complement(14248..14985)
                     /locus_tag="ONT65_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR2329249.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ONT65_001637"
     CDS             complement(15138..16478)
                     /locus_tag="ONT65_001637"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR2329250.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ONT65_001638"
     CDS             complement(16715..18238)
                     /locus_tag="ONT65_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR2329251.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="ONT65_001639"
     CDS             complement(18616..19227)
                     /locus_tag="ONT65_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2329252.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="ONT65_001640"
     CDS             complement(19557..20273)
                     /locus_tag="ONT65_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2329253.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ONT65_001641"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ONT65_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR2329254.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="ONT65_001642"
     CDS             complement(20832..21629)
                     /locus_tag="ONT65_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR2329255.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="ONT65_001643"
     CDS             complement(21599..21970)
                     /locus_tag="ONT65_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2329256.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ONT65_001644"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ONT65_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR2329257.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6469 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctggt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaaaa
//
LOCUS       DAJSBC010000039        22487 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSBC010000039 DAJSBC010000000
VERSION     DAJSBC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.039"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..449
                     /locus_tag="ONT65_001645"
     CDS             <1..449
                     /locus_tag="ONT65_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2329258.1"
                     /translation="HHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKN
                     FFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMD
                     FRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(520..1830)
                     /locus_tag="ONT65_001646"
     CDS             complement(520..1830)
                     /locus_tag="ONT65_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2329259.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1842..3260)
                     /locus_tag="ONT65_001647"
     CDS             complement(1842..3260)
                     /locus_tag="ONT65_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329260.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3500..5068)
                     /locus_tag="ONT65_001648"
     CDS             complement(3500..5068)
                     /locus_tag="ONT65_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2329261.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5072..5353)
                     /locus_tag="ONT65_001649"
     CDS             complement(5072..5353)
                     /locus_tag="ONT65_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2329262.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5350..6273)
                     /locus_tag="ONT65_001650"
     CDS             complement(5350..6273)
                     /locus_tag="ONT65_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR2329263.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6270..7148)
                     /locus_tag="ONT65_001651"
     CDS             complement(6270..7148)
                     /locus_tag="ONT65_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR2329264.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7135..8472)
                     /locus_tag="ONT65_001652"
     CDS             complement(7135..8472)
                     /locus_tag="ONT65_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2329265.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8475..9578)
                     /locus_tag="ONT65_001653"
     CDS             complement(8475..9578)
                     /locus_tag="ONT65_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2329266.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9652..11160)
                     /locus_tag="ONT65_001654"
     CDS             complement(9652..11160)
                     /locus_tag="ONT65_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2329267.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11126..11818)
                     /locus_tag="ONT65_001655"
     CDS             complement(11126..11818)
                     /locus_tag="ONT65_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2329268.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11840..12814)
                     /locus_tag="ONT65_001656"
     CDS             complement(11840..12814)
                     /locus_tag="ONT65_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2329269.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13114..13986
                     /locus_tag="ONT65_001657"
     CDS             13114..13986
                     /locus_tag="ONT65_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2329270.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14568..16766)
                     /locus_tag="ONT65_001658"
     CDS             complement(14568..16766)
                     /locus_tag="ONT65_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329271.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16998..17990
                     /locus_tag="ONT65_001659"
     CDS             16998..17990
                     /locus_tag="ONT65_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2329272.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18050..19471
                     /locus_tag="ONT65_001660"
     CDS             18050..19471
                     /locus_tag="ONT65_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR2329273.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19464..20963
                     /locus_tag="ONT65_001661"
     CDS             19464..20963
                     /locus_tag="ONT65_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR2329274.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21002..22396
                     /gene="uxaC"
                     /locus_tag="ONT65_001662"
     CDS             21002..22396
                     /gene="uxaC"
                     /locus_tag="ONT65_001662"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2329275.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6828 a         4581 c         3893 g         7185 t
ORIGIN      
        1 ttcatcataa aagtgccgtc attactttag ttgaaagatt atctaaagtc attatcacga
       61 ttaaacccaa cggccgtaag gcattagata ttgaaactgc ccttaatcaa tggttttctc
      121 gcttccctaa aaacttcttt aaatctatta cgtttgactg tggaaaagaa ttttctaact
      181 ggaaagccat tagtaaccaa catgatattg atatatattt tgcggaccct ggaacacctt
      241 ctcaacgccc attaaacgag aattctaacg ggattctgcg tcgtaatgga ctgccgaaat
      301 caatggattt tagaaaagtg aatcagacat ttatttccag tgtcagcaat caacgtaatc
      361 atattccaag aaaatcattg aattacagaa caccaatcga aatatttttg agctatgtac
      421 aagaagcatt ttattctagc ttaatttgac aaatcatata tcattaaaaa aaataaaggt
      481 caagctaaaa tattatttaa gcttgacctt attataattt caacgattgt caaaagtatt
      541 gtttgcagct gtgttgccag tttctataga taaatcacag cttttgaaac ataagttttg
      601 tactgcttcg attcgcccga attcttttgc agttatttgg caatgagaaa agcagacatc
      661 ttctataggc ttttcaggaa atgctactaa atcaaaagct cgggaaggaa gtgagtaacc
      721 tggagaaagg catgcctgta cgtttttgat gtgtatgtct ttgactttcg tcatttgctt
      781 tgattcaggt atttcttcag ccactgcttg ccaactttct ggcatttgag caaggtcatt
      841 gctatttttt ctattgtatt cattatgcca gtccattatc caagaaaaag gaaattgtac
      901 atcaatcata ttcagatttt ctaccctgat attttcgatc actccccctc tttcttttga
      961 agatttcatt cgaataccac agtctgactg aaaaaaattg atatcgtgaa tatatacgtc
     1021 actaactcca gcactaactt cgctaccaat agttacaccg tatcctgaac ggattttaca
     1081 tctagcaacc tcgattcttg atgattttcg attgactctt gcaccattgc catctctgcc
     1141 agacttaata gcaatacaat catcaccaca ggccaaatca cattcataga cacgcacatt
     1201 tgttgaagaa tcaatatcaa tcccatctgt acttggtcca tcattgttgc ggatgattac
     1261 tttttctact aatacatcgt tagaataagt gatttgcaag ttccaaaaac ctgatttctg
     1321 aaaaatcaaa tcacgaacaa caacatgttc tgcatgatac agtaaacaag cgcgtggacg
     1381 tttaatagca taatccgcaa tccaacgaag tcccttttga tcgtattcag ctcttgtccc
     1441 gcttttttga tcttttcccc aatacagctc ccaccaatgg tctccttgac cgtcgattat
     1501 tccttttcct tctataagaa tatctttcgc cgagagaaca ttcaaaatcg ctgctggcca
     1561 tttcatttct acgccggcta ctcgtgtatc gatttctgga aatgcttcga tatctgttct
     1621 ccccttgagg actgcacctt cttcaaagat aagatgactc ttatctttta aaaaaagggc
     1681 tccgactaaa taaatgcctt ttggaataaa tacaggtaag tgttctttcg caccttgatc
     1741 gattgctttt tgcagtatta ttgtatcatc ttgttgagtg tcgtttgttt taaagttcaa
     1801 agcattgata atcccttttt ttgtccgcaa tacgtttctc cctatctttc attgattttt
     1861 ttcaattctt caccaataaa tacagttttt gaacgattca gtgggtaacc tactagcaaa
     1921 atgattgcgg atacaaataa gcagaatgct ggtaacaagt ttgccatcgt ataaatcctt
     1981 tcttgtacta aaactgattg gatggctcca cctgttgtag acgattggta accaatgagt
     2041 gccagcataa atccgcctac cgctcctgca catgcttgtc ctacttttct cgcaaaagaa
     2101 tttactccat atactgttcc atcttctctt aagcctgtct tatattgatg ataatcaatc
     2161 acgtcagtaa tgaatgccca gaccatcaaa ttgaataaac cagatcctaa agtagcgata
     2221 aataaaagaa tcagatatac ccatgcattt gttatatgaa ggaaatacat cagcaagtac
     2281 atacatgaag aagcaagtaa cgcaaatacg cttgcttcct ttttaccaaa taattttgtc
     2341 agctttgtag caaatggtgc taaaactact actgttccgt aagtaaataa aagagcaaca
     2401 gacattgctt gtttattatg gaagtaatca ttgaataaat aagtcatatt cgtcccagct
     2461 aaaatctgat tgatcacaat gaaaatgtca acaatgacta aaataatcag tgctcgatta
     2521 gctaacaaac cttttaccaa ctgagaagca gatacttttt cgtttttgac aactcttact
     2581 ctttcagtag tcaatttaca agttaacata tatgcgacaa atgctattgc agcacatccg
     2641 attgaaatcc aaaagaaatg attccctgaa acgacttgtt gtccactctt gttatgggta
     2701 tacattaaaa tcggaatgaa gaatcctgta gttgatccgc caatcgcaga accaatgctt
     2761 cggaacgtgg acaacgaggt gcggtcatta ggatttgagc taattgctgc tgccattgct
     2821 ccatacggga tattgattgt tgagagaaaa atcccataaa caatgtatga aacaaaaata
     2881 taagcaatct tcaccgtcat tggaagatgt tggacgaatg gcaaaaacaa aatgacagtc
     2941 actaagcaaa atggatactt catttttctg atccaaggaa taaatcggcc agtttctgtt
     3001 aactctgaga catcacataa tcttccaacc atcacatcag caaacgcatc caaaattctc
     3061 gaaatcaaga ataacgtacc tacgattgcc cctgaaacgc caagaacatt cgtataataa
     3121 atcatcaaga aactattgac taaacctaaa ataaaacagt tacctagatc accgaacatg
     3181 tagcctatct tatcggaaat cccaaattcc tttttttctt tttttactgt atttgctgta
     3241 acagcatttt gtgaaagcat ttgaattcct cctacctctt ctagtaaacg ttttcaaaat
     3301 tataactctt tttttcttgt ttttatattc acaatgttgg ctttttattg cattatcttg
     3361 acagataaaa aacaaaaaat aatctaaaaa cattgatgca taagattgat aacttttttt
     3421 acataacaat atatacaaac aaaaaaattc actaactagt gaaaataagt tagtgaacct
     3481 catttattaa agaattaaat tacagatgat cttttccatc atacttgaaa aactcaaaat
     3541 cagcataact tcctcttttt ttatttagat catgcactgc tatcccgata aaatttccag
     3601 taaaccctcc tgagagtata cacatgtttt tttgcggacc tactttcttc cattcttcag
     3661 ttgatacgtc tttcaaatga aactgtgctg tctctttctt tacagtgact ttcaaaaaag
     3721 tcttcttttc tgtgagtgga atcgtccaat cttctatttg tgctactccg ttttctgatt
     3781 ctatcatacg taggcatttt cccttgtcct cattccaagt aacataacaa taaatataat
     3841 tggattcatt taagtacagt accagtccag ccatctgatt aaaattatta ggcatgtatt
     3901 caaatgctgt ctccgcatca aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt
     3961 cgaaggtaga ttgaagagat tcaccggaaa tcagacgtaa aaaacctggt ctacattttg
     4021 tatcacacca atcagcattt ggcaaaatgc gtctggcatt ccagttcttc tttagctctc
     4081 cttcaaatgt atccataaac tgatgatctt tttcttgttg tacctcttca tttgtctcaa
     4141 tcattatttc ttccatcgga gttgtctggc tatctttaag tcttaaccaa tcgtcggacg
     4201 tccaatacac ttcttgaata gcggtttctc ttcccaaaat agccgcaccg tttaatggtc
     4261 tggtacaaag atatgtcatg taccatcttc ctgacggggt ttgtactaag ctcccatgtc
     4321 ctgtacattg caatgtagaa gcaggtttgt cactagctgt cagcatagga aaaccaggat
     4381 caagctcgta aggaccagta atattctttg ctcggcaaac tgtaactgaa tgtcctcttc
     4441 ctgtaccgcc ttctgccgtc atcaaataat aatacccgtt acgaaaatac aaatgtggtg
     4501 cctccgtttt cgctaattct gtacctgcaa atattttata gatgggacca tcaagcttct
     4561 tcttctctgc atcatattct tgtaatacga taccgctgga tttattcggt gtgtccagac
     4621 gataatccca tagttcattg acaagccatt tttttcccgt ttcaagatca tggaacaaag
     4681 aaggatcaaa gccgttacta tttaaataaa ttggttcgga ccaaggacct gtgatctcag
     4741 atgcagtgat caaataattg tggacatctt taaacggacg gcctgcactt ttgacatctg
     4801 tgtaaacaag ataaaacaat ccatcagcat aactgatctg tggcgcccat atacttccat
     4861 ttgtaggatt tccttgcaaa gatacttgag tggtcaaaat atccgttgta tgtgaccagt
     4921 tgactaaatt tttcgactca tatactcgga ttcctggaag ccattcaaaa gaggagacaa
     4981 taatgtaata ccgctctttc actcttaata tattaggatc tggattgaat cctcttaaaa
     5041 taggattttt cactttgata gtctgcatga ttcaggctcc ttttctcatt cttactcgat
     5101 ataaactgaa tgccccagct gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5161 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5221 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5281 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5341 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5401 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5461 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5521 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5581 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5641 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5701 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5761 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5821 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5881 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     5941 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6001 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6061 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6121 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6181 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6241 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6301 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6361 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6421 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6481 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6541 ccatgatgat ctaggcggcg atgtagcaaa agtgtttcga tgtccacctg tgccgctaga
     6601 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6661 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6721 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6781 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6841 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6901 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     6961 aggtaaagga ccgtcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7021 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7081 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7141 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7201 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7261 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7321 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7381 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7441 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7501 gaatgctgat gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7561 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7621 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7681 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7741 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7801 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7861 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7921 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     7981 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8041 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8101 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8161 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8221 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8281 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8341 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8401 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8461 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8521 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8581 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8641 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8701 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8761 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8821 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8881 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     8941 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9001 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9061 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9121 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9181 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9241 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9301 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9361 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9421 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9481 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9541 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9601 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9661 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9721 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9781 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9841 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9901 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
     9961 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10021 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10081 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10141 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10201 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10261 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10321 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10381 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10441 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10501 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10561 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10621 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10681 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10741 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10801 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10861 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10921 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    10981 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11041 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11101 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11161 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11221 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11281 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11341 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11401 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11461 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11521 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11581 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11641 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11701 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11761 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11821 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11881 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    11941 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12001 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12061 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12121 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12181 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12241 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12301 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12361 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12421 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12481 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12541 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12601 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12661 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12721 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12781 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12841 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttatc
    12901 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    12961 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13021 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13081 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13141 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13201 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13261 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13321 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13381 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13441 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13501 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13561 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13621 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13681 ccattagctt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13741 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13801 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13861 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13921 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    13981 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14041 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14101 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14161 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14221 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14281 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14341 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14401 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattaaacgg cgtttatctt
    14461 gttgattctg cggcaataaa atcagccagc agcaaaacat gaagtgctgt tttttgctgt
    14521 cctcttttat tgcttgaatc agatcacaag aaaaacagcc tcttgattca caaatatttt
    14581 tttcgatatt ctcccggcgt aatcgattcc ttctttttaa agaaacgaat aaaattttgc
    14641 ggatttcgat attgcaatct ttctgcaatc tcttttacgg agaggttagt ctctttcaac
    14701 catttttttg ctacctctaa acgatgattt tgaacaaaat caccaaattt ttctcctgtt
    14761 tcctttttaa atacattact taaatagtta gggttataat gcaacctatc agcgatcaca
    14821 tctaaagaca gatcacgatc atattctgtg tagacgatat gcataatatt ctccgacaaa
    14881 tttttaaatt catgatcagt gattgcttga gagcattctg tgattggcag aactaattgt
    14941 tctaaaacaa gtttttctag ttctttggga tgataattgt tcagtgcttt tacataaatt
    15001 tgtttcatgt tatcaaagtt ttttgaatct actcccaata actgtcctaa ttgaacgagt
    15061 tcattgatca agcggaccga aactacttcc cgactcaaag gatttttatt ttgtgcaaac
    15121 aatgcatcaa tcaattcatg tactaaattc ttcacttcct gatttccaga acgaatggct
    15181 tcaaacaatc gattttgttc ttctatcgga tatctaataa gtgttttttc atgagcagca
    15241 ggaacgattt cctgataaaa aataattgaa cttggaccag tatttacacg ataatacagg
    15301 gcttcttttg cccgatcaac cgattgcttg ctttcattca gcgtttcgaa ccggttactg
    15361 atgcctatac tgatagttaa attcaagtat tcttttacac gtttttgaat gagtcggcaa
    15421 tactccatga cttttttgct tgcttctgca tcatctttat ccattcgata aatagtggct
    15481 tgcatttctt cattcaaaac gatcggcagc attcgatttt cctttggcac aatttccgtg
    15541 atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc caccgtgact atcttctaac
    15601 agatcgattt gaaccaaagc tgtataatag cagtcatttt gagaactata accaaattgc
    15661 tgcaaccgct gatctagttc tcgcctttct actcgatttc taaataaact aagtacaaat
    15721 agcgtttcca gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc tttttctcct
    15781 atgatccgac tgactgattg agcgaccaaa tctagttctt tcccactgaa tccttttgat
    15841 ttaagattca gacgttcctg tatttgagaa atcggacggg caaatctgtc cgaaaataag
    15901 taggaaaaaa gaccaatgag tcctaccagt gttaaagaca ctgcaactag cccgattctc
    15961 agattgcgga tcattgcgcc aattgcacta gagtcaactt ctaatctgta aatccaatta
    16021 ttataatctg atttcagtac tatatacgtt ttatcttttt ctcttatcac tttcagctga
    16081 ttgtaatcta gatccacgtc cgttaataca gttggttgct ctttattttt tactgtctga
    16141 gtagtaaaaa ggttgccaac ttcgttttca atagaaagta tgtcttctcc actttcacca
    16201 acaacttgtt ctattgtatg tttctctatc gtagcaattc ccaatgcata tttttctttt
    16261 tcaaacattg gcaaagaaat caccatttcg attcctttgt ttttcttttg ccaaaataaa
    16321 ttatttttgt tatttatgta cttctgttga tacttttcaa cttcctcttc ggtcagttga
    16381 ctcaacgatc cattgattac tccccattta ccagccaaac tgactaaatc ataattatta
    16441 tcatccataa caatcgtttc tataaaattt agttctttcc tgatttcaga ataagtggag
    16501 taatctgtat atgtcagcgg atgcttgaat atgtccgaga aatttttggt cgaaccatac
    16561 gtagaaaacg aatattcaat cgttctaatt ttccattcaa cgttagtttt aacttgcctg
    16621 agcaaattct ttttgtctgt taaataactc gattcgatcg attcataggt attctggtaa
    16681 atgaataaac taaatcctat gacaagtacg gctcctaata aaaacatcgg taaaaatatt
    16741 ttataaaata ctgtactttt cttcattttc ttcctctcct ccttttaaaa taaaaggctt
    16801 tcatttttct gtaaaattga tactcatcta attaaatgag cattccccgc tttcaagaat
    16861 agcataatta aaaataaaaa tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa
    16921 gatctattga cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa
    16981 gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc taaagcagct ggcgtttctc
    17041 atacgacagt ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa acaaaacaaa
    17101 aaatacgaca actagctaag gaaatgaatt attcgccaaa ctatagtgca aaaggtttag
    17161 tgaatcaaaa aaaatatcta atcggactat ttttctcaag tttgcataaa ggaacttcat
    17221 caaatttcct agcagattcc attgcaggca tccattcagt attagacaca aattacagtt
    17281 tatctgttga aagtatcgat gttgttgatt taacagaaat caacctgcaa cgctacgatg
    17341 gactgatcgt tatgagtcaa agtgatgatg atcagccttt catcgactat ctcaaagaaa
    17401 aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt atcaacgtca
    17461 ctgccgatga tgcggccggt gttatgcagg cagtagatta cggtattgga tgcggacata
    17521 cgaagatcgc ttatattggc ggaaagaaaa attttcgttc gacaaaagaa cgggaaaaag
    17581 gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa tctagcctat tttttaacag
    17641 gagattacag cttagaaagc ggcctccaaa acatggaata cctattatca ctttctgacc
    17701 tgcctactct ggttttttgc ggcaacgacg acatagcgat tggtgcattg agagctgtac
    17761 atttagctgg cctccgtgtt ccccagaaca tttctctgat cgggttcgat gattctccag
    17821 tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa gaaatgtgtc
    17881 aagaaggaac aaagctttta ctagatttga tcgaaggcag gacgatcgaa caatctaaaa
    17941 ttgagctctc caccttgttg atccttcgtg agtcgatcaa ttacttgtaa actacaagta
    18001 attttatata aaaatgttaa cgtgtgcata acacagaaag gaagtttata tgaaaaaact
    18061 gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt ttggtgaagg
    18121 caattttatg cgtggattta ttgattggca gattcagcag ctgaataacc aacaattgtt
    18181 taaaggtaat gttgcaatca ttcaaccttt agaacaaggt cttggaaata tgatgaacca
    18241 gcaagataat ttatatacgg tcattttaca aggactcagc aatggcgaaa tcgttgatac
    18301 ttccgagatt atcaccgtag tagaacaaat gatcaatcct tatgatgaat gggatgctta
    18361 tctatctcta gcagaaaatg acgacttggc ctatatcgtc tccaatacaa cagaggcagg
    18421 aatccaattc gtggagactg ataatttaac agatactccc ccacagagct tcccgggcaa
    18481 actgacagcc tttttatacc gacgtttcaa attaaacaaa gctggcttta ccatcattcc
    18541 ttgcgaactg attgacagaa acggcgaacg tctaaaagaa atcattttac gctacgcaga
    18601 aaaatgggat ttagaacctg cttttgttga gtggttagaa aaagaaaatg ttttctgttg
    18661 tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg ttttcaatca
    18721 acggcataac tatgaagatc aattgatggt caaagcagaa ccattcatgc tttgggtgat
    18781 cgaagggcct aaagaattgg agaatcaact accattcaaa caggctggct tgaatgtcat
    18841 catcacagat gatatgactc cttatcgtga acgtaaggtg catttgctta atggaccgca
    18901 tacagcgatg gtccctcttg ctttgttggc ggaattggaa actgtagaag aagtaatgaa
    18961 agatccttta ttctccgcat atgttgatca gttattcaat ctagaattga ttcctatgct
    19021 ttctttacct aaagatgaat tagccattta tgctgaccaa atcaaagaac gtttcttgaa
    19081 tccttttgcc catcacaaat tagaagcgat ttcattaaat agtgtttcaa agttcagcac
    19141 ccgtctgctt ccagtgttca agaagtatat cgaagaacaa aatcaggtac caccgttaat
    19201 cactgtttca ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa agccacatga
    19261 tgatgaaaag acgataagcg aatttactga tgcttggtct gataatggaa cagctattcc
    19321 tcgattattg caaaacgctg cgctttgggg agaagatttg tctcaaatac cgaatgtgac
    19381 agatacagtt caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt cagtattaca
    19441 agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca accaacaatg aaattaaatc
    19501 gacgagacag tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa ctgacgattg
    19561 atcagcaaac gatcaccact cttgaagata tcccgcaagg tcataagatt gcattgggtg
    19621 atttgaaagc tggagacaac gtgatcaaat atggctatcc aatcggtcat gtgacgacag
    19681 acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt tctggtgaat
    19741 tggattatcg atatgaaaaa gatgtccatc caagtcatta cctttttgag aaccgaacat
    19801 ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg taatgatttg tttttagtac
    19861 ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt caaacagttc aaggaaaaac
    19921 atccagatct gggacagttt gaccatatta cgattttaaa gcatccttat ggctgttcgc
    19981 agcttggaaa agatcatcaa aatactcgag aaattttagc ggacgcagta aatcatccca
    20041 atgccggagg agttttagta tttggattag gttgtgagaa caatacagtt cctgaattca
    20101 aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt tctagtggct caagatgttt
    20161 acgatgagat cgaacaaggt gtggctctat tagaagagct tttaacagcg gcggaaaatg
    20221 accagcgcat atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc ggcggttctg
    20281 atggtctatc gggaataact gctaatcctt tacttggtgc attttctgac tacttgattg
    20341 ctcaaggagg cagcacgatc ttaacagaag tgccagaaat gtttggcgca gaacaagtat
    20401 tgatggcacg agcggaaaat gaagaagttt ttgactcaat cgttgacttg atcaatgact
    20461 tcaaacagta tttcttgtct tacggggaac cagtttacga aaacccatct cctggtaata
    20521 aagcaggtgg gatcactaca ttagaagata agtctttagg ctgcacgcaa aaatcaggaa
    20581 gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca gggcttagtt
    20641 tattgcaagc tcccgggaat gatcttgtag ctgcctctgc tcttgcttca tccgattgcc
    20701 agttggtctt gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc ccaacggtca
    20761 aggtatctac gaatacaact ctttttgaac gcaaaggtca ttggatggat tttaatgcag
    20821 gcgtcttatt agaacaacca atggaagtaa tcttagaatc gtttgttcaa aaaatcatcg
    20881 ctgtagctag tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa attgccattt
    20941 ttaaaaacgg agtaacactt tgattttcat aaaacagtat aaaaagaaag aaggaagatt
    21001 tatgttttta tctgatgatt ttttattaaa agacgattgg gcgaaaaagc tttaccattc
    21061 ctacgcaaaa aacatgccca ttattgatta tcactgtcac ttagatccaa aagaaattta
    21121 cgaaaacaac aatttcagca atctgacaga agcttggtta ggaggcgatc attacaaatg
    21181 gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg cttctgattt
    21241 tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc cgctttacag
    21301 ttggtcacat cttgaattga agcgtttctt cggtattgac ttgttgataa atgaagaaaa
    21361 tgcagagaaa atttggacac aagcaaatga aattttggca gctcctgatt ttcgccgtcg
    21421 ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca acagatgatc ccgcagacga
    21481 cttgcactat catcagttgc ttgctcatga agaaccagat ttgaaggttc taccttcatt
    21541 ccgtccagat aaagcactca atattgaaaa agaaggattt aatgcatggg tcacactact
    21601 ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag tcttagggca
    21661 gcggatagaa tacttccatc aacatggatg tcgtgtttcc gaccacgctt tagacatctt
    21721 acgctataaa gaagcggatg aaacagtatt agaagagatt tttcaaaaag cacgagctaa
    21781 caaactatta tcttcagatg aaatcgatgc ttatcttaca gaaacactga tacatttgac
    21841 ccacttctat cattctcata attggacgat gcagctgcac atccatgctt atcgtaactg
    21901 taatacacaa atgttggaaa aattagggcc cgataccggc tatgatggaa tgaatgatct
    21961 ttctttgact attcctctgc aaaaattatt gaaccgtgca gaagaaacgg atcagttgcc
    22021 aaagacgatc ctttattcat tgaatccgaa tgactatcct gcgattttag ctttgatggg
    22081 ctgtttccaa aaagaacaga atggaaaact gcagttaggt tctggatggt ggtacaacga
    22141 tactcgagct ggtatgcgcg atcaattgac acaatttgct gatggtagtg ctttggggaa
    22201 ttttgtcggc atgctgacag attcaagaag tttcctgtcc tacactagac atgaatattt
    22261 ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg cgaggagaag cacctgaaga
    22321 tgaacaactt ttaggaagtc tagttcaagc gattagttat acaaatgcta aaaattactt
    22381 tggctttttt gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag gatagtgaaa
    22441 taacggaatc cgtttatcca cgttttagag aagaagacgg accaaat
//
LOCUS       DAJSBC010000040        21988 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSBC010000040 DAJSBC010000000
VERSION     DAJSBC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.040"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT65_001663"
     CDS             <1..395
                     /locus_tag="ONT65_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329276.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ONT65_001664"
     CDS             398..1147
                     /locus_tag="ONT65_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329277.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ONT65_001665"
     CDS             1159..1428
                     /locus_tag="ONT65_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329278.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ONT65_001666"
     CDS             1433..1597
                     /locus_tag="ONT65_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329279.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ONT65_001667"
     CDS             1590..1892
                     /locus_tag="ONT65_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329280.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ONT65_001668"
     CDS             1889..2050
                     /locus_tag="ONT65_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2329281.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ONT65_001669"
     CDS             2047..2352
                     /locus_tag="ONT65_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329282.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ONT65_001670"
     CDS             2352..2708
                     /locus_tag="ONT65_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR2329283.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="ONT65_001671"
     CDS             2695..2913
                     /locus_tag="ONT65_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329284.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ONT65_001672"
     CDS             2910..3329
                     /locus_tag="ONT65_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2329285.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ONT65_001673"
     CDS             3326..3883
                     /locus_tag="ONT65_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2329286.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ONT65_001674"
     CDS             3880..4176
                     /locus_tag="ONT65_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329287.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ONT65_001675"
     CDS             4253..4666
                     /locus_tag="ONT65_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2329288.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ONT65_001676"
     CDS             5012..5386
                     /locus_tag="ONT65_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329289.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ONT65_001677"
     CDS             5423..5590
                     /locus_tag="ONT65_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329290.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ONT65_001678"
     CDS             5616..5960
                     /locus_tag="ONT65_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2329291.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ONT65_001679"
     CDS             5965..6246
                     /locus_tag="ONT65_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329292.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ONT65_001680"
     CDS             6349..6663
                     /locus_tag="ONT65_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR2329293.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ONT65_001681"
     CDS             6641..8335
                     /locus_tag="ONT65_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2329294.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ONT65_001682"
     CDS             8355..9533
                     /locus_tag="ONT65_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2329295.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ONT65_001683"
     CDS             9496..10182
                     /locus_tag="ONT65_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2329296.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ONT65_001684"
     CDS             10182..11342
                     /locus_tag="ONT65_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2329297.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ONT65_001685"
     CDS             11352..12227
                     /locus_tag="ONT65_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329298.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ONT65_001686"
     CDS             12224..12535
                     /locus_tag="ONT65_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329299.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ONT65_001687"
     CDS             12525..12878
                     /locus_tag="ONT65_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR2329300.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ONT65_001688"
     CDS             12868..13269
                     /locus_tag="ONT65_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329301.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ONT65_001689"
     CDS             13262..13666
                     /locus_tag="ONT65_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329302.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ONT65_001690"
     CDS             13678..14286
                     /locus_tag="ONT65_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2329303.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ONT65_001691"
     CDS             14306..14668
                     /locus_tag="ONT65_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329304.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ONT65_001692"
     CDS             14671..14853
                     /locus_tag="ONT65_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329305.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ONT65_001693"
     CDS             14870..18289
                     /locus_tag="ONT65_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2329306.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ONT65_001694"
     CDS             18340..19077
                     /locus_tag="ONT65_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2329307.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ONT65_001695"
     CDS             19087..21378
                     /locus_tag="ONT65_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2329308.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ONT65_001696"
     CDS             21402..>21988
                     /locus_tag="ONT65_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329309.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAJSBC010000041        21526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSBC010000041 DAJSBC010000000
VERSION     DAJSBC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.041"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            40..300
                     /locus_tag="ONT65_001697"
     CDS             40..300
                     /locus_tag="ONT65_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329310.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            341..568
                     /locus_tag="ONT65_001698"
     CDS             341..568
                     /locus_tag="ONT65_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329311.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            633..1205
                     /locus_tag="ONT65_001699"
     CDS             633..1205
                     /locus_tag="ONT65_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329312.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1206..1517
                     /locus_tag="ONT65_001700"
     CDS             1206..1517
                     /locus_tag="ONT65_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2329313.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            1508..1768
                     /locus_tag="ONT65_001701"
     CDS             1508..1768
                     /locus_tag="ONT65_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329314.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            1929..2288
                     /locus_tag="ONT65_001702"
     CDS             1929..2288
                     /locus_tag="ONT65_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329315.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2285..3928
                     /locus_tag="ONT65_001703"
     CDS             2285..3928
                     /locus_tag="ONT65_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2329316.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            3963..5045
                     /locus_tag="ONT65_001704"
     CDS             3963..5045
                     /locus_tag="ONT65_001704"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329317.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5089..6261
                     /locus_tag="ONT65_001705"
     CDS             5089..6261
                     /locus_tag="ONT65_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2329318.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6248..6952
                     /locus_tag="ONT65_001706"
     CDS             6248..6952
                     /locus_tag="ONT65_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2329319.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            6957..8117
                     /locus_tag="ONT65_001707"
     CDS             6957..8117
                     /locus_tag="ONT65_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2329320.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8193..8471
                     /locus_tag="ONT65_001708"
     CDS             8193..8471
                     /locus_tag="ONT65_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR2329321.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8452..8823
                     /locus_tag="ONT65_001709"
     CDS             8452..8823
                     /locus_tag="ONT65_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR2329322.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            8839..9243
                     /locus_tag="ONT65_001710"
     CDS             8839..9243
                     /locus_tag="ONT65_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329323.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9240..9650
                     /locus_tag="ONT65_001711"
     CDS             9240..9650
                     /locus_tag="ONT65_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329324.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            9711..10298
                     /locus_tag="ONT65_001712"
     CDS             9711..10298
                     /locus_tag="ONT65_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2329325.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10371..10838
                     /locus_tag="ONT65_001713"
     CDS             10371..10838
                     /locus_tag="ONT65_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR2329326.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            10884..11192
                     /locus_tag="ONT65_001714"
     CDS             10884..11192
                     /locus_tag="ONT65_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329327.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11387..18121
                     /locus_tag="ONT65_001715"
     CDS             11387..18121
                     /locus_tag="ONT65_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2329328.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18125..18997
                     /locus_tag="ONT65_001716"
     CDS             18125..18997
                     /locus_tag="ONT65_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCR2329329.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            18994..20043
                     /locus_tag="ONT65_001717"
     CDS             18994..20043
                     /locus_tag="ONT65_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329330.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20047..20871
                     /locus_tag="ONT65_001718"
     CDS             20047..20871
                     /locus_tag="ONT65_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329331.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            20889..>21526
                     /locus_tag="ONT65_001719"
     CDS             20889..>21526
                     /locus_tag="ONT65_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCR2329332.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7736 a         3219 c         4559 g         6012 t
ORIGIN      
        1 atacatcaac agctggttat gcgcttgcgg aggtacaaaa tgaatcatga gaagtttatc
       61 gaaaaatgca aggctattgt acgtgaaaga attgaaaatg agattgctga cctaagtgga
      121 gcagtgcctg agtttagtat tttcatagtc tggtcatgca aaacactgca aaatagcaaa
      181 gcattagtca gcgctagctt aaaaggagca ccgtattttg aaattacgtt gaacggggac
      241 aaaggtgaaa tctatgtaga tacttatctc aaaaaatcaa atgaatgtat caaagtctag
      301 caggtgctag gctttttctt tacataaagg aggctgcata atgagaaact actggtatgt
      361 atcactaact aatgaatatc ctcgaaccat tggtgattgt tcagtgcgtg ttgtgcgttc
      421 tgtacaaatc aaagggaagt actctattgt cgaaatgcta agagaagcta caccaaacga
      481 agtggataaa tgcaagctga tatattgcgg tcatggctat tggaaagacg agtatatcca
      541 atacaacatt gagaggtaca tcaaatgaat gataactatg attacatcaa gttgttagca
      601 agtaattatt agatgttttt aggaggaaaa ctatgaaatt aaaagattat atcagagaag
      661 ggtataacgt tgtaactgca cccaatctag cttacaaaat tcaagaagat tatcccaatg
      721 ctttagtgtt tactgataga gctttagatg ccattcctat agggaagtta ttagtagatc
      781 gtttttatag caataatcca gctgttctta gtgctaagcc acttcaaaac gcttatgcta
      841 ttgaacgctt tacttgcgag tttactggat atgagacggt agtaccaatg acaggtgaga
      901 caggaagaaa agtaattcaa cagatcaaaa agaggattgc tgaattgacg ttagatgatg
      961 gaagtaatac gaaccttcta gaaatcaaac taagagatac tgactcagta ccagaagttt
     1021 attataaagg tcaacgaatg gatgagatgc cgataggtct tgtcgatatt acttatcatt
     1081 gggatacgga agaatatgaa tgccaagtac caaaaggagc taacgacatc actattgaat
     1141 attatgattc acataataac aagtacttag atagaaaaat cattgggcac aagagagata
     1201 tgtaaatgat tgatgtttca agtaaacaag caagagctag gttctatgga tcaactgagt
     1261 ggaggaaact tagaagtcaa tgcttagagc gagatcatta tgagtgccaa tggtgtaaag
     1321 cagagggtag actaacaact caacatgatt caattcttga agtggatcat ataaaggagc
     1381 tggagtatca tccggattta gctctagata taaacaacct aagaacctta tgcaaagatt
     1441 gccataacaa aaggcacaac cgtatgaaat atcgtggcca acaaaagaaa agaaagtggg
     1501 atgatgaatg gtggtaagaa aaacagcaaa cagagatgat gtcaaacgag cagaagcaat
     1561 cgctatggtt gataaggcta ttgataagtt ggttctattt aagagtcagc tcattgaagg
     1621 aacaacaact gtagaggata tatctatcaa caatagacta ataggtaacg ctaatgaagt
     1681 aacagggaca tttaatatta atgtagacat tgattatacc ttgagtccat ttgcacatat
     1741 tgtagaaagc aacgagcaag acggctgata aatcagtata aagcgaagat atatagagtg
     1801 aaataccccc ggtcaaaaga ttttgagaag aaataaaatt tttgggaacc ggtggatggg
     1861 gtcaactctg caaataaatg atgctatttt cacacgaccc cctccccacg tagaaaggaa
     1921 gtgagttgat ggctgatctg caagagagaa ataggcttgt ttctgctgaa gaaaggcgtc
     1981 taaataaatt gtttaaggat attgataagg ataaaaagaa ggtcgtttct ggtttgatta
     2041 cgcaagcggc acgcttaaaa attcttcttg atgaaatgtg gattgatatt tccgaaaaag
     2101 gtgattatga gcttttctct cagtcagaaa atcaaaaacc ttacgagcgg gaacgaccag
     2161 ttgctaaaca gtataattcg agagaccaag catatcaacg tgtaattaag caacttactg
     2221 actatttgcc agaagaaaag cgcgaaccta caaaaaatgc agcgctagat ggtagtgatt
     2281 tgctgtgaca acattaaagc cattctattt tgatgaatac gtagatttat acgaaaaagg
     2341 ggtaatccct tttaataaag aaagaattca gttaattact tatttaaaaa acgaagtgct
     2401 agttcgggat gatatctatt ttgatgaaga aatgattcaa aagtttatca agtacactga
     2461 aaaaaatttt tttcctttag ctaaatatca aaaatttatc acacctttca ttttctgcta
     2521 tcaaaaagag gacgacgagg tgttttttga tgaaattcta aatagtattg cgcgaggcgg
     2581 cgggaaaaat gggttcatgt cagcaagaga ctcatttttt attagccctc tctacggtgt
     2641 cagaaattat gatgtaacga ttacagcgaa ttctgagaag cagggaaagg tatcttttaa
     2701 agaagtttat gaaatggtac aaatgcgcaa actggaaaaa caattttatt tgactaagat
     2761 ggctatagtc aacaatgtaa ctaattcgat ttttagttat aggacaaaca atcctaagac
     2821 tatggatagt gcaagagatg gctgcttaga atttgatgaa atccatcagt ttgaatcttc
     2881 agacctagtt gatattcagc gtagtgggct agggaaaata aaacatcctc gaacttttta
     2941 taatggtaca aatggtcatg ttagagaagg tttttatgac aaaatgttgg aacgagcaca
     3001 aaagattttt agtggagaga acaagaatga tcgcttgttt ccctttattt gtaaactgga
     3061 taacattgat gaaatggata atcctaaaat gtggtcaaag gctaatccaa tgttcgaaga
     3121 agattcagca tattcaaaac gtctgttttc tacagtgatg aaagaatata aaaaactaga
     3181 agatgagcca tcaggtaggc gtgaatttgt tgttaaacga atgaatttta cagaaggtga
     3241 tgcagaatca gatattacga ctcatgaaaa attaatggct actaaacaag aattgccaaa
     3301 tttgaatggt cgttcttgtg tggctggatt tgattatgca agtattcgtg attttgcaac
     3361 agtgggtttg ctttttaaaa ttgatgataa atttatctgg cagcaacata gttttgctag
     3421 gaaaaagttt ctagatacat tcaaactgaa agcgcctatt aaggattggg agaaaaaagg
     3481 tttagtgaca atcgttgatg agccatcaat tgatcctcgt catttaattg attggttaaa
     3541 tgaaaaaaga aaaatccatc aaatagaaat cgtttgtgcc gacggatttc gtatggattt
     3601 gctgaaacct cttctagaga aagaatcata taattttgag tttatcagaa atataagagg
     3661 tgtgcagtca aaagttgctc ctattattga agatggtttt gcaaatgaac ggtttatttt
     3721 tggtgatgat cctatgatgc gttggtatac aaataattcg tttatcaaag aagacaaatt
     3781 agggaataaa acatttttga aaaaagagcc agtaaggcgt aaaacagatg ggtttcatgc
     3841 ttttttggca gctttatata aacgagaaga aataactgat atggattatg aggaagcatt
     3901 tgatatgttg gatgagattg aattttaagg tatactcctt attgaaaggg gtagttatag
     3961 aagtgaaaaa gagatatttt ctatttgtta caatatttat aataatagca atttgtgttt
     4021 tgacagtatt tttaccacaa attactgagt atttaaatta tcggatattt accacaaaag
     4081 tagatgatag cgttactagt ttaagtaggc tagaatttat aaaaatatgt gcatctgtgt
     4141 ttggtcctat tttgactgta atggtatttg tgaatacttt aaatatgcag aaaaaaacag
     4201 agcaaaagca aaatgagctt gaagtaaagc aagctaaggc tgagaacgag agagcaaaaa
     4261 aagagctgtt aacacagata gatcgtgagt tctatttttt attaaatatg tttattgaaa
     4321 tacaaaaaga tcaaaatgcc aaacaagctg ttattgcttt acagaagttg gcaattttta
     4381 acgaaaaggg ttttaatcaa gactatgcat ttacttctaa agatttatca gatagtgaaa
     4441 ttgggagtac aattgaacaa tcaggaccgc tgaagggcac agttgaatat ataaaatttg
     4501 aaacatttga tgtaagcaag tcgatgggga attatcaaac aaaaacaaaa gacgttgcta
     4561 caagtttaca cgatcagaaa gtagctatat tgaatgaaca atatgaaaag atgcaaatat
     4621 atcttggacg gtacttcaaa atgtttcacc gaatagttaa aacattaaat gagtattatg
     4681 acgattataa tgattttgat gtgaagaggt atacaaaata tatagggaca ttaagaacac
     4741 aaatttctcc agctgaattc caagtgattt tattcaattc tctatatata aaacgaggtt
     4801 ttggcttagg aattcaactt attggaagtg gtttttttgg agatgatttt gattttgaaa
     4861 ctaatcaaca ttttgaaaca agtatcaatg aacagtggtt tctatcacta tcaactgttg
     4921 acagtgataa ctctataaag cgacaaaaat tatctgaata tattaaagaa ttaggatccg
     4981 tagaatataa aaaaatagct aattttgaaa gtttatataa actctttaat gaaactaagc
     5041 tctagaaata gggctttttt atttttcttt gaaaggtggt gaaaacatgt gagcttattt
     5101 gatatcttta agcagtcaat aagaaatgaa gagccttctg agtggatacc ggattttgta
     5161 tatggagagg aagtatctgc tcgggcatac ctcaaaatta tggcaaaaaa tactgtttta
     5221 gattttgttg caagaactat gtcaacgtta gaagttaagt ttaaaaataa agatgggaca
     5281 tcagactggg attatattct aaatgttcga cctaattctg atatgtcagc agctacattt
     5341 tggcaaaaat ttttttatcg gctcttagat gacaacgaag tgttagtaat atttacagat
     5401 gataatcaat tgctgattgc tgatgatttt agtcgaacgg agtatgccgt gtatgatgat
     5461 gtttttacaa atgtgatggt aaaaaatttt gtctttgaac gttcatggaa catgtctgac
     5521 gtcatttata tggaatataa taacgaagaa ttagaaaggt ttacacgagg tttatttgaa
     5581 gattacgccg cgttgttcgg acggattcta gaagttgcaa tgcgtaacaa ccaaattcgt
     5641 gggtctgtat ccattgattc aacgggaacg gcgaatgatg aaaaaggaaa agatggtaag
     5701 actagatctc aaagattaca agaatatatt gataggattt ggaactcttt taaaactaac
     5761 tcagttgcaa ttgttcccaa agtgaaaggc tttgagtatg aggaatacac caataaacaa
     5821 ggcgtatcga atcaatcgct agatgaactg aataagatga agtcgtcttt aattgatgat
     5881 gttgccaacg ccataggagt acctacggcg cttatttatg gtgaaaaagc tgaactggat
     5941 tcaaatatta aagcttttcg taaattgtgt attgctccac tagttaaaaa acttcaagac
     6001 gaattggtgg caaaaataat tagtcgtcaa gagtacaaaa acggggaacg tatagaagtt
     6061 aaaaaagttc tccaaaaaga tctattagag tatgctactc aaattgataa aataacatct
     6121 ggtggtgcgt tttatattga cgaagttcgt gatgaagttg attatgatga gttgccaaat
     6181 aatgaaggaa aaattcgtat tcgtacgaaa aactatgaag aaactgtgaa gggaggtgag
     6241 aacgacaatg ccgaaagcta aaaaagtacc atttcagttt actaacgaaa ttcagaatgg
     6301 taagcacatt ctcaccttga gtggaaatgt acagaaaaaa tattggcgtg atgatgatgt
     6361 cataaacgct aaagatattc gtgactctct agatactgta accgatgata ttgtaatcaa
     6421 gttgaacagc ccaggcggtg atgtgtttga agggattgag atttacaatt atttaaaaga
     6481 tcacccatca aatattactg tagaagttac agggcttgcc gcaagcgcag caacgtttat
     6541 tgtcgctggt gccaatgatg tgattatgaa tgttgggact tctttgatga ttcatgaagc
     6601 ttctacattt gcttggggga ataaacagga catccaaaaa acgttaaacg cattggaaac
     6661 gatcgatgat tctatcttag ctatttattc agataagact ggccaatctg ttgataagtt
     6721 gcgtgaatgg atgaatgaag aaaaatggtt tacagcagat gaagctgtca aatatggatt
     6781 tgccaattct gtgaagcgtg aagaaccaca aaatgaacct caaaatattg cagcgatgat
     6841 tcaagatgct gttgccgttg caatagcgaa tttaaaggaa cctgttacta ataaaatcga
     6901 acaagaatca aaacaaaaat cattaatcgc acgattgcga aaaggagaat aaagatatgt
     6961 taaaaattac agataaaact gcagatgcaa aaaaggcatt taatgctatt tcttcgaaag
     7021 aagatgcaac acccgaacaa attaatgctg ctctagaaga atatgtaacc gccattgcag
     7081 aagatgccgg caaacaggtg cgtgctgaat atgaagaatt gaaaaatgtg acggacaacc
     7141 gtgtattaga ggctcgtgga attcctacac taacagcaga agaaacaaag ttttataatg
     7201 aagccgtaaa atctggaggt ttcgattctg acgtagtttg gccagaaacg attttggaac
     7261 gtgtttttga aaatcttcaa aacgatcatc ctattttagg cctcattaac tttacaccaa
     7321 ctgtaggtcg tgtcaaagta attagagcac gtcgtaaagg tgttgctgtt tttggaccat
     7381 tgcataaaga ccttgaaggc caactagatg ctgaatttgg tgcaactgaa tttgttcaac
     7441 ttgcgttgac cgcatttttc ttaatttcta atgatacctt agatttagga ccacgatgga
     7501 ttgaccgttt tatcaatctt tctttaagtg aagcagtacg tgatgtttgg gctaaaaaaa
     7561 ttattacagg ttctggtaaa gatgagccaa ttggattgct taaagatcta gatggtgcgg
     7621 tcacaagtgg tgtatatcca gataaagcta gcgcagggac attaactttt gcgaaagaga
     7681 agattgttct tgaacttgca ggggtgatga aaaaattatc taaatacaca tacaaagtag
     7741 atggtgacga tgaaggtgaa gtgaaatacc gttctgttgc tggacgagtg tatctaattg
     7801 ttaatcctgt tgattactgg gatatcgtag ctagagttac atttgcaaat ttaaacaatg
     7861 tgtatgggac tacgatgcca tttatcgctg ttgatcatat catcgaatct gtagacgtac
     7921 cagaaagtaa atgtattgca tacgttgata aagagtatga agctactcaa tcaagagctg
     7981 aaaaagtcta tgtatataaa gaaacttttg caatgaaacg cgcaactttg tatgctgtcg
     8041 acatgcttgg taatggctat ccaacaaata atgatgcagc acaagtatat gacttggact
     8101 ttacagcggg ggagtaatgt cccctgcaat tggtgagata acaccgacga ctgatggggc
     8161 agtcattaat ctttcgtaaa tggggttaga gtatgaacac tgaagaattt ataaaagaat
     8221 ataaggcccg atttcgtatt ttccatactt ccgaagatga ggatatcggg aaacagttag
     8281 agagcgggtt tgccgatata aaatctctaa tcggcagttt tgatccttcc acttatgaga
     8341 aaggaaaaga attagtttat gaacgtactc ggtatctacg gaacgaatca ctagagtact
     8401 tttacgacaa ttttcaaaca atgattatgg atgcatcaat tgatctggca ggtgctggat
     8461 atgccaatta aatcaaatta taagaagcct aaaattgtag ctggtgatct aaatacgcca
     8521 gttacttttt ttgagtttca acctaataag ggtccagaac ctggtgatga agaaaaaaag
     8581 gaactgtatt tttgtacagc actagtttac aacccatcaa tgaaagatcg tgacattctt
     8641 ggcacaaaag aaggcgttac gataaaaata cgggatcccc atcaatctta tattcctact
     8701 aacaaacata aagtaaggat attagattat cgatatggag ataaagtttg ggaaataatc
     8761 gatgtctcac cggatttgga agaaaattca attgtaaaga tagtattaga attaattact
     8821 taaatttggt gataaatgat gagtaacgta aagtttgaag gcgttcaaga aacaatccaa
     8881 gctttagaaa aaaagtatgg agaaaagaaa actaagacat taacaaaaaa agcaatcaat
     8941 gttggcgctg aaaaagtaga aaaacagttg caaacggaca tgaccgtttt taaagacaaa
     9001 ggttatacga tcgctgaggt tgttcgcaag aacgctactt atagagatta caaggcagaa
     9061 gctgaaatcg gttggaatgg tccacatcaa cgatatagaa tcattcattt gaatgaatgg
     9121 ggatatacac gaaagggcaa acaaattaga ccgagaggat ttggtgtaat aacaaaatct
     9181 cttaagaatt ccgagctgtt gtactttgat gctgtaggag aggaggttaa aaagagttta
     9241 tgaaagatat gcttaatatc atttacgagg ctctttgttt gaatgagtat attcattcaa
     9301 tgacctacaa cgagaatacc gagcaatatc gtattaaatt ttatgagcaa cctgaaaccg
     9361 ccgataaaac aggtccaatg atcacaattc gtcctgtaga tacacctaac gaagcgtacc
     9421 acggaagtaa caaagagctt tctgtggagc atatgataca gattgacgtt gaatcgaaat
     9481 atagagcaac atgcaagcag attcaatatg agataaaaaa agagatgaaa aaactaggct
     9541 ttggccaaat ttccggtcaa gggttagatg aatactttac tgaaacaaac cgctttgtag
     9601 acgcgcgtcg ttacgaagga aatacaaaaa tttacgatac cgaatattag aaattaagac
     9661 acgaaaataa cgtgtctttt tttattaccc aaaattagga ggaaaatttt atgactttag
     9721 taggattcaa aaaaatgaca atcggggtct ttgacagcac agggaagatt ccagcagcaa
     9781 atttatatgt aattgaaggt gaacaaaaca aaggggcgac tgtatctgct gaaatcagtg
     9841 ggctatcaaa agaaccatca aaagtttacg gatcgaacat cgcttactat gtctctcaaa
     9901 aaggaacagg cgatgtttca gctacttttg gattgttaga tttaccaaca gaagtaaatg
     9961 acaaaattct tggttacaaa accgatacaa acaaaatcag cttcttggga gaagatactg
    10021 agccaccata ttgtgcaatt ttgatggaat cggaggattt gaacggcgat acagcaatgc
    10081 ttgcgatgtt caaaggaaaa ttcagtcgtg aatctattaa tcttaacact acgacaaatg
    10141 aagctttcga accagaagca gaagaatatg tattttcagc gattgccaat gatgttgaag
    10201 gtgatgctaa aggccaaaca gtcgcaaaat atgttggaga cgaagaagct tctattactg
    10261 gtttgaaaga gatggtattt cctgcagcgg gggagtaact agccccgttg ttggagcaat
    10321 cactcccact attaatgggg caacagttga attaagttag gaggataact atgccagata
    10381 catttaaaat ctataaaaaa aatggtactg aatttacaaa agtcgcagag ggtgaaagtc
    10441 cactttctat tactggaatt gcagcaaata ctcaagttgc taaaggagat tatcaagcta
    10501 cgcgtgtggt cggaggtcaa gaatcagtaa aagtagatat tcctgcattc accactcttc
    10561 caatttcagt tactggtgtg acgttggata aaacgacagc tgaagttgaa caaggcggaa
    10621 ccttaaaatt aactcctact gtaacaccag caaatgcaac agacaaaaca ggaagctgga
    10681 gcagttcgaa tacagctgta gctacggtta ctggaggcaa cgttacagta aaaacagacg
    10741 ctgaagttgg cggaactaca gaagtttcat ttactactac ggatggcggt aaagtagcaa
    10801 aatgtactat tacagttatt gaaaaagcag cgggctagtc ttcggactag cttttttatt
    10861 tggaaaataa tggaggaatc taaatggcaa aagtaagaat tgaattgaaa gaagaatcag
    10921 gaaagaaagt ctatgaaaac atagacacaa cagggaaaga ttatcgaaaa gctttagaaa
    10981 caatcaaaaa gctaaatgaa gacggagtaa tgatttggaa tcgtttagac atttatttgg
    11041 attttgctgt aggtatcttc agggatagca aattgacagc tgaacaaatt cttgatggct
    11101 taccatctga gaaggtaatg agcacattgg acgacatcct aggagaagta atggggattg
    11161 aaagtaatcc agacccagac gcaaaaaagt aacacccgaa gaagcagaag aaatgtactt
    11221 aaacctatgc agagagttag tgaaagcggg atggtcacta tctgacatag aagataattc
    11281 ctttgaaatg atgatgaaaa ttgcgtgcac ttcgcctaag aaagagaaac aaaaagaagt
    11341 tgatcttaaa gacttcttga aatccatgta gagaggaggt aaacatatgg caaatggaaa
    11401 acctttaggc aatatgatta tcaaactaga cttagacagt tcagcttttt caaaaggtct
    11461 agccggagct aaaaacgctg ttaaccatca aatgaaagca atgaaatcac agatgcaagt
    11521 aatgaactct tcaggaaatt ctctcggtgc tttacaagca aaatacaatg gtttaggcgg
    11581 agttctacaa gcaaatgaaa agcaagtaga attgctcact aaggcttata aggatagttt
    11641 tgactctaac ggaaacgcta ctgcttcgac tgctaaatac gctaatcaat tgaatcaagc
    11701 aacagctaga tcagcaagtt acgaatctca gatgaaaaca actgttggac agattgctcg
    11761 gatgaaagtg gaaacggaag ggtttacggg gaaactaaag gctaattctg atgtattgat
    11821 taattctggg aaaaaaattg aagcctttgg taaaggagtt tctgggatag gatctgccct
    11881 aactgttgga gtaacagctc caatagtggc aggcgcaact gctgttacta gtgcagctat
    11941 ttcatgggaa agtgcttttg caggtgtaaa aaagacaaat gatgaagttg tagactcgaa
    12001 tggaaacgta gtttattcct atagcgactt agagaaaggt ctgcgtaatc ttgccaccca
    12061 attacctggt agccatcaag aaatagcaaa tgtagccgaa gcagccgggc aattaggtat
    12121 aaagactgaa aacgtagtta gcttcactaa aacgatgata gacatgggtg aatcgactaa
    12181 tatgtcggct gaaactgcgg caacttcttt agcacgattt gctaatatta cccaaatgtc
    12241 tcaaaaagac tttgataaat taggatcagt cattgttgat ctaggtaaca attttgctac
    12301 aaccgaatca gaaatcactg aaatgggatt gcggttagcg ggagctggta aacagattgg
    12361 aatgagccaa ggcgaaattc taggatttgc tactgctcta tcttctgttg gtgtcgaagc
    12421 tgaagcgggt gggtctgcct tttcgaaggt catgatccag atgcaactag ctgtagaaaa
    12481 aggatttggc gcttttgatc agctgaaaca gatggcagaa gaacaaggtg ttccgtgggt
    12541 taatttagta aatgctgtga gagacggtgg aaaatcgtta aaagcagtat cagagcaaat
    12601 gggattcact tcatctgatt taaagaaaat gtataaagaa gcggacaatt cgaaaagttc
    12661 gcttgaaaat tttgcgaatg tggcaggtgt tacaagtgaa caattccaga agttgttcaa
    12721 gagtgatcct tcagaagcaa tcatcaagtt cattcaaggg ctgaaagatt cagaaaaaca
    12781 tggtacttca gcaatcaaag ttctggatga catggatata aaagaagttc gactacgcga
    12841 cagtttattg agagctgcaa atgctagtga catattcaaa ggagcaattg aaaaaggaaa
    12901 cggcgcgtgg aaacaaaata cagctttaac aaatgaagct aataaaagat acgaaacaac
    12961 tgaatcaaag ctaaagatat tgaaaaacga agtgattgat acagcaatcg atcttggagg
    13021 tccatttgta gacgcgttgc gtgatggtct aaatgtatcg aaaccattga taaaaaactt
    13081 agggaatatg gcgaaagctt tctcagctat gagtgatgat caacaaagaa acattatcaa
    13141 atgggttgga ttagcagcag cagctggtcc agcgctgaag cttttaggaa ctggtacttc
    13201 agtaataggt aaagttactt caactactgg aagattgacg aaaagccttg ttgatttagc
    13261 agcgaaagca gcagaaaaga aggctatgga tgcctttgca tcgtcggtaa caacagctgg
    13321 tgctgcagct gcaaagacag caggagctgg aggtattggc gggttagtcc ctatgatggg
    13381 aaaaacagct agtgctgcaa gtactgctgc gggcgccagc ggaataggcg caatgacaac
    13441 gtcactaggt ttgttaggtc ctgctctact tggtattgtt ggcgttggtg gcgcactggc
    13501 agttggctat ggagcatgga aattatttgg tgaagaagct tggaattcct cgcaaagagt
    13561 aaagcaatgg ggaacagatg tcggtgagga gatagatagt actcttgacg gagtacagaa
    13621 gaatattgaa ggtgcaaatg gtcaattcgg tcttctcaaa gagggtttta ctgttgatga
    13681 tgcaaataat atggcggcta atttcgcaac cgctggaaca tctttggaaa catctttaac
    13741 gggtagaata aaggcattag acgatgcact aaaagggcta cctcaatcag ttcaggatgc
    13801 catgaaagaa gtagctgaat cagaagaaga aaccttaggg caatctcttt ctaaaataaa
    13861 agacaacaat gaagaaataa aaaaaatcag agaaagagcc gcaaatcaga acagagactt
    13921 gactgtttca gaactacaac agatacagga tctaatgaaa ggaacttctc aagcgtatgt
    13981 ggatacgctc gatgtttctg ctaaagacag aaaaaagata ctatcagcca tgaatggtga
    14041 tgttgcacaa gctacagaag aagaagcgaa gacgtggtta caatctttag cgaaacaaaa
    14101 agctgcaact accgaaaatt atagacaaat gcttgaagaa aaaaaggcgg ctttaaagga
    14161 aaagggagta agcaaagaat tcattgatgc attagaagat gaaatgaatg attacatcaa
    14221 aactacaaat caaggattcg atagccaaat agctgctata acaaataaat atccacaatt
    14281 gattaacgaa gtgagtttat caaatggtca attatttagc aaaacggcag ctgctatgga
    14341 caatactggt cagtatacaa aagtttttct cgaaagtaat gaaaagattt tagcccaagc
    14401 gaagaaaaca tcggatgaaa tagcagataa tgccgaaaaa aatgcagaac aacttggtat
    14461 ggtagctgat gaggctacgc atgctggatc aacttggaat ggcttagttt tagacgaaaa
    14521 aacaggaaaa gtaaaaacta atgtacaaga agtagtgaat gaagcaacga aggatttcac
    14581 taaatggaat gaattgaaac ctgttgttca tgatgcgaaa ctaacaacga atgcaaaaga
    14641 tgtcatttcc atagcggcag tacaaaatgg ctattgggat gatatggaat ggaaagaaaa
    14701 agaagcatta ttagaagatg agtgttctaa aaatgtatac aaggctttag aaaatgctgg
    14761 taaatggaat gagttagata ttccttctaa agaagcaatc ctaacttcaa atactcctga
    14821 agttatgggt gaaactctca tgaatttggg attatgggat acttacaaac ttcaagtgaa
    14881 agatttagat ttgaatgcgt ataaagtata cgatgctatc gatggatcag aagaaaaatt
    14941 aaatagttgg gctcaattag ataaccccac aaaagaattg ttattagata actcagattt
    15001 ttctacaaaa ctttttaatt ccgaagaaat gtttaaacgt tggaatcagt tatcatctaa
    15061 tgcaaagttt ctttcggcta ataatactaa atttacagag gttcttttat catctgacag
    15121 aatgtggaat caatgggtaa accttcctga taatgagaaa agattacttg cagataacga
    15181 agatttagct accactgttt tatcttcaga agaaatatac ggaagatgga ctcaattacc
    15241 agataatcaa aaatatatgt tagctgacaa tactgactta gcaaacaaaa tattcgcttc
    15301 agaagaatca tggaatgcat ggaaagcaat tccagacaca gttaaaaact tgcttggtaa
    15361 taatattgat ttactggcta aagtgaaaga cgggactgta agtgtagaag aatataacaa
    15421 agttatgcca cttcttaaaa ctctatatgg agataacgtt aatttaaaag aggcaatcag
    15481 tggggctaaa agctctatag agaattacaa caaaaatatt tttcctagtg ataaaactgc
    15541 agtaggacac gatgaggctt cacaagctgc taaagatgca tttgaagcgt tcaacatctt
    15601 ccaaacaaaa ataccagata aaattactaa aactgtatca gctgacttta taggtcctat
    15661 gccgaaaaat gcgaaaggaa caaacttcca tcctggcgga gcagctatgg taaacgatca
    15721 aaaagggcct acttatgaag agcttataac cttgccaagc ggagaaactt ttatacctaa
    15781 aggacgtaat gtcgttttag acttaccaag aggttcgaaa gtactgaacg ccactaaaac
    15841 taaacgtcta gtgcctaaat atgctgatgg tataggaaac ataacgacgg tttcatctac
    15901 gctaaactta gatgctttaa ttttagctat taacgagcta acgacggtat tgagaactca
    15961 actgatggca ggaacgactg atgctaaaga tactaaacta tcagatgaaa caaaagttgc
    16021 cgatccgatt attccggaca gtctttctga aaaatcagat cagtatctag gaattggagc
    16081 tcaatggtta actaatctaa tgaatggttg gaactcagcg gttcctcaat atatgaatag
    16141 tgaaatggtt tttattacaa actatctcaa tgcgctaaaa cttcaaaaca atcctaatta
    16201 tacgcaagga gcaacatgga ataagaactt actgaatgga tggaatagtt taactgggac
    16261 ctttattgct acaatcaatt cattttgtaa tcaagcaatg gtcacgctta gaaactacaa
    16321 cactcctatg tacaacaatg gccgaacgtg gcagcaaaat aatcttaatg gttggaattc
    16381 attgtatgga tcattcatag cccgtgtaaa tcagcttggt aacgattcga ttaacaacct
    16441 tcgttctaaa agtggtggtt tttacaatgc agggacattc ttgttgcaat ctttaataaa
    16501 cggaatgaat tcgttaggta attctctttc tactacaatg aacaatgtcg ctaatacaat
    16561 ggttggtggt ataggcaaag gagttaatgg cgtcatatcg ggagttaact atgtgcttaa
    16621 ggaagtcgaa tcaagtaaga atatcggaaa ctggccaatt ccacaatacg ctaagggaac
    16681 tgatggacat cctggtggac ttgctatgat taatgatcaa aaaggcctag ttcacgaaga
    16741 atatgttcag atgccagatg gacgtagttt tattgccaaa ggtagagatc ttcttgttaa
    16801 tttgcctaaa ggtgctcagg tcttaaatgc ttctttaaca aagaaattaa aggaacgttt
    16861 aaatattcca cactacgaaa aaggtattgg caatttagat attgttgatt tgttagatga
    16921 tgaaaaaaga atgttagaat tcttaactag caaagttgat ttttcaggca ttaatgaacc
    16981 atggcttgat atgactaaat caggaacgtc attaatgtcc aaagctgcaa atacaatgct
    17041 tcaatcaaaa ttaagtgaat tcttcactca tggcaatttt gatggtgcag taaatgccaa
    17101 tggtgtttat caatatttag ttgatgtggc acagaaagta atgggtaagt tccccggact
    17161 tacagtaact tctggatatc gagcaggaga tgcctactat cacggaaaac ggcaagctat
    17221 cgacttggct tatccaggta tttctggaga tccgagatat acagcagcag caaattacgc
    17281 ttttgaaaaa ttcccttcaa aaattgcgta tgtcattacg aatggacgtg tacgtgaccg
    17341 catgggatta tctggaacaa ggtcaagcgg acaatggacg aattggccag atggcgatca
    17401 cttcgatcat attcatttaa acggttcgat ggggtctggc gatatattca atggaggagt
    17461 tggaggaagt ggtgttgcac gctggcgttc ttatgtgtct aaagcattga aaatgaatgg
    17521 attaccagct acagcagcgt atataaatgc ctggatgtcc caaatccaaa cggaatctgg
    17581 tggtaatgag aaagcaatcg gcggcaatga tgggctcgcc gaaggcaatg caacaggatt
    17641 acttcaaacg aaacccggaa cttttgcagc taacgctttt cctggtcacg gaaatattat
    17701 gaacggattt gataacatgt tagcagcaat caattatgct aagaaacgtt acggagttgc
    17761 aggtatgttg caagttatcg ggaaaggaca tggatatgcc aatggcggct tgattaccaa
    17821 agacggacta tatcgagcag gagaaggaaa taaaccagaa atggttattc cattgacaag
    17881 aaaaacaaga gctatcgaac ttatgggtca agcattagcc ttcctttctg gaagcaataa
    17941 aaaagaaacg aaaagtgaga ttaattctgt agataataga catatcctac aagcgataga
    18001 aaaacagtct cgtatgacaa atgatctgct atcattatta ctctcgtttt ttaaaagtaa
    18061 caatcgatca gataaagaac tagctttaga tattcagaaa attttaatca ggaggacata
    18121 gtatttgagg acagtaatat taagaaataa attgaatgaa gaaattgatt tgtccacaga
    18181 agattatttt gctactgaaa tgtcgaatct aggttttgaa gttacaaaag agcatattgg
    18241 acaatggggg agtttcagag aaataggaga gagtattgaa ttgtctgaat ttcaatcatc
    18301 agttatgatt tctgttcacg gatttagaga acaacaatta tatggttcat tagttgaatt
    18361 tctggctaat ggaccttttg aactagaatt ttcttttgat tcagaaacaa tgatcaggaa
    18421 atgtagtttg aaatcattat ctaaaacaga aattgattcc caaagctcat tgctaacaga
    18481 tactttgaat ctatatttta cttcaagctg gtattctatt aagcgtgaaa aattaataca
    18541 gaattcaact tctctcaata cacgtggaaa agtttactca tttgttcgac catatgtcta
    18601 caccaaaaat gtatgggaaa agaaaggtgt atttaagttc agtaataatt ctgtatattt
    18661 gacaaatagt acagagcgaa tgtctccgtt aaaaataagg attattggag agtgctcaaa
    18721 tccatattgg gaagttatcc aaaactctaa agtagtagct tcagatggct attttattaa
    18781 tatgaaagac acacaaatat tagaagtgtc tagtttgcta gaagaccaaa cggcaatttt
    18841 aaaagatgta ggtggagttg aatcttctgt ttatcaacaa caagatttta ctaaatcaaa
    18901 cttcgtccaa gcgcctctag gagaattcag cgttgttttt catgttggac atgcaaatgt
    18961 agagattgaa ctttatgagg agcgtgatct attttgattt tagccattac cctctttcac
    19021 cgagatatga atttatataa cgaacgttta atttcgactg agttcgaatt tggaacggat
    19081 gaaattggag aagaagtaag taattttatt acagataaat atgtccatgt aaaatcagga
    19141 gattttctac tagcaaaatt tattcctagc gggaagattg cttattttgg agtgattagc
    19201 tcgcaagaag acaaaaaaat aaattgcaaa ggattactca gtttggccga tagcgagata
    19261 ccgactgtaa aaatatcagg tgctaattat gaagagcata ttcgacgact gattgaatac
    19321 tacttattga acgatccaac aaaacaacta aaaaatattt tggaggtaaa aacagaaagc
    19381 gttacttctc attcttatca agctgttgac acaaataaga gacgattgaa tgcatatata
    19441 ataaatgggt tcaaaaaata taatgttaaa tggtatgtca aagagattaa aggtaaaaaa
    19501 atatatactg gtattcgagc agttgatgat tcaatttata ttaaagataa ttcatctgag
    19561 tttaatgact gggatgtttt tgtgcaatct cctggaccag ggaacgagaa caaattattg
    19621 atcgtagata aagcaatgaa agatatagaa aatcccttgg tattgtctac gtggtattta
    19681 gatgacgaaa ataatcttac gcaagattca acgaataaga acattgcatt gcctaccgtt
    19741 aatttagtaa atatatatga tcaaactcaa gaagatagac cttcttatga agaggtagct
    19801 aaatcggagc ttaaaggcaa cgcctattca catgaaatta aagttaatgt tgtgctaaat
    19861 gctagaaata tcaatgtaga aaatatcgaa acaggaatgt tagcgactat tgtttacaaa
    19921 gagcagactt acaaatctgt tctaactgcc tggcgaatat ccagcagtaa aaagaatata
    19981 gaattgacat ttggtaatgt ccgaagtcgt tttatggatt attttgaaga taatggggga
    20041 taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt aagcgcagaa
    20101 aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat tgatggttac
    20161 gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg
    20221 attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt tccagcaaac
    20281 tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc tggatctatt
    20341 cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga atttataaca
    20401 aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc actatgctca
    20461 gttaattcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta cgagctgact
    20521 ctctctaaag gagaaatttt gtggaggggg actgcgttaa tgcatgatac tcaaactgtc
    20581 caaccttcaa aaaaaatttg gcagacagtt tcaggttttt tgttagtatg gctcccatac
    20641 gaaaacgggc aagcaattga ggatagatat gtaactacgc ctttttataa ggagcgtata
    20701 acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca caaaaagtgg
    20761 tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc agcaagcaat
    20821 gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta gttaggaggt
    20881 atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag ccttatctac
    20941 gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat gtacaacttc
    21001 ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg
    21061 gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat tcgactaatg
    21121 ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa ttcaaacaag
    21181 catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac ttcatcatag
    21241 acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt tcttcattgg
    21301 aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat aaacaggtcc
    21361 aatacgatca aatctactcg aaatataacg aattagtaag attgataaat gaatcagata
    21421 agcaagtaaa tgatcgtatt gataaggcaa ataatcgtat tgatcaaacc aatcagcaaa
    21481 tcggcgatct cggcaagctg aaaaagatgt acagtaacag cattga
//
LOCUS       DAJSBC010000042        21415 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSBC010000042 DAJSBC010000000
VERSION     DAJSBC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.042"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            96..797
                     /locus_tag="ONT65_001720"
     CDS             96..797
                     /locus_tag="ONT65_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2329333.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1406..2422)
                     /gene="tsaD"
                     /locus_tag="ONT65_001721"
     CDS             complement(1406..2422)
                     /gene="tsaD"
                     /locus_tag="ONT65_001721"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR2329334.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2419..2883)
                     /gene="rimI"
                     /locus_tag="ONT65_001722"
     CDS             complement(2419..2883)
                     /gene="rimI"
                     /locus_tag="ONT65_001722"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2329335.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2890..3432)
                     /gene="rimI"
                     /locus_tag="ONT65_001723"
     CDS             complement(2890..3432)
                     /gene="rimI"
                     /locus_tag="ONT65_001723"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2329336.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3416..4138)
                     /gene="tsaB"
                     /locus_tag="ONT65_001724"
     CDS             complement(3416..4138)
                     /gene="tsaB"
                     /locus_tag="ONT65_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR2329337.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4329..5309)
                     /locus_tag="ONT65_001725"
     CDS             complement(4329..5309)
                     /locus_tag="ONT65_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2329338.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5333..6817)
                     /gene="galT"
                     /locus_tag="ONT65_001726"
     CDS             complement(5333..6817)
                     /gene="galT"
                     /locus_tag="ONT65_001726"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR2329339.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6829..7818)
                     /gene="galE"
                     /locus_tag="ONT65_001727"
     CDS             complement(6829..7818)
                     /gene="galE"
                     /locus_tag="ONT65_001727"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR2329340.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8066..8233
                     /locus_tag="ONT65_001728"
     CDS             8066..8233
                     /locus_tag="ONT65_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR2329341.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8295..10106)
                     /locus_tag="ONT65_001729"
     CDS             complement(8295..10106)
                     /locus_tag="ONT65_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR2329342.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10103..10468)
                     /locus_tag="ONT65_001730"
     CDS             complement(10103..10468)
                     /locus_tag="ONT65_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR2329343.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10631..11026)
                     /locus_tag="ONT65_001731"
     CDS             complement(10631..11026)
                     /locus_tag="ONT65_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2329344.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11044..12006)
                     /locus_tag="ONT65_001732"
     CDS             complement(11044..12006)
                     /locus_tag="ONT65_001732"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR2329345.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12006..12218)
                     /locus_tag="ONT65_001733"
     CDS             complement(12006..12218)
                     /locus_tag="ONT65_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR2329346.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12239..12937)
                     /locus_tag="ONT65_001734"
     CDS             complement(12239..12937)
                     /locus_tag="ONT65_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR2329347.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12957..13499)
                     /locus_tag="ONT65_001735"
     CDS             complement(12957..13499)
                     /locus_tag="ONT65_001735"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR2329348.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13631..14635)
                     /locus_tag="ONT65_001736"
     CDS             complement(13631..14635)
                     /locus_tag="ONT65_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2329349.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14632..15621)
                     /locus_tag="ONT65_001737"
     CDS             complement(14632..15621)
                     /locus_tag="ONT65_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2329350.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15618..16424)
                     /locus_tag="ONT65_001738"
     CDS             complement(15618..16424)
                     /locus_tag="ONT65_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329351.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16590..17546
                     /locus_tag="ONT65_001739"
     CDS             16590..17546
                     /locus_tag="ONT65_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2329352.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17623..18141)
                     /locus_tag="ONT65_001740"
     CDS             complement(17623..18141)
                     /locus_tag="ONT65_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2329353.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18229..18378)
                     /gene="rpmG"
                     /locus_tag="ONT65_001741"
     CDS             complement(18229..18378)
                     /gene="rpmG"
                     /locus_tag="ONT65_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2329354.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18606..19052
                     /locus_tag="ONT65_001742"
     CDS             18606..19052
                     /locus_tag="ONT65_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR2329355.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19247..21142)
                     /gene="asnB"
                     /locus_tag="ONT65_001743"
     CDS             complement(19247..21142)
                     /gene="asnB"
                     /locus_tag="ONT65_001743"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR2329356.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6439 a         4549 c         3697 g         6730 t
ORIGIN      
        1 taaaacttgt ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca
       61 ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc
      121 acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac
      181 agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg
      241 gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg
      301 atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt
      361 caggaatgtt tggagaagag acctacactt atacagctca agcgatgact gatattgaaa
      421 cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga
      481 tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg
      541 gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat
      601 tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat
      661 tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg
      721 attccgggct gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc
      781 cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgataaat atcagggtca
      841 ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc actggacaag
      901 actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact
      961 gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc gatattcctc
     1021 gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata aataagctgt
     1081 aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc tttgacaaaa
     1141 acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt
     1201 cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta
     1261 aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata
     1321 agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat
     1381 gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca
     1441 actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg
     1501 cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag
     1561 ctgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta
     1621 taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat
     1681 cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct
     1741 gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat
     1801 aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta
     1861 gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat
     1921 aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt
     1981 gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga
     2041 atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg
     2101 gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga
     2161 caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt
     2221 ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt
     2281 caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata
     2341 agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg
     2401 ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat
     2461 cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc
     2521 tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag
     2581 cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc
     2641 ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta
     2701 tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg
     2761 ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc
     2821 gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat
     2881 caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc
     2941 agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg
     3001 tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc
     3061 aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg
     3121 aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat
     3181 cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga
     3241 tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag
     3301 cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc
     3361 gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa
     3421 ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt
     3481 ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag
     3541 cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca
     3601 tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga
     3661 aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta
     3721 catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat
     3781 tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg
     3841 cgatggtttt cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt
     3901 ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga
     3961 ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca
     4021 tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt
     4081 cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat
     4141 atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga
     4201 ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa
     4261 attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt
     4321 tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt
     4381 gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc
     4441 cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca
     4501 tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct
     4561 ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga
     4621 ttcgttttta aatattgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg
     4681 ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg
     4741 ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata
     4801 tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg
     4861 acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt
     4921 tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca
     4981 tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct
     5041 tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc
     5101 gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga
     5161 tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca
     5221 acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc
     5281 aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa
     5341 tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt
     5401 cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat
     5461 ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag
     5521 ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac
     5581 gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat
     5641 gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt
     5701 ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg
     5761 tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg
     5821 ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt
     5881 gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt
     5941 ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct
     6001 gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa
     6061 caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga
     6121 ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag
     6181 gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg
     6241 tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg
     6301 taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg
     6361 gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga
     6421 tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat
     6481 actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg
     6541 aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca
     6601 attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag
     6661 attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta
     6721 aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa
     6781 gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata
     6841 accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt
     6901 gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct
     6961 aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct
     7021 agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt
     7081 gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc
     7141 tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac
     7201 agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc
     7261 tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg
     7321 caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat
     7381 caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga
     7441 ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa
     7501 ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa
     7561 tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat
     7621 cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa
     7681 atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac
     7741 ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc
     7801 tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt ttgttgattc
     7861 tatataattt aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt aaaatttcgt
     7921 aaattcatat ggtaaattta taataaaaca tttagtaaac attttcaagg gttttttgca
     7981 aaggtttaca aacaaacgta aaactagcct ttttttctct tttgttccat aatagatata
     8041 acaaataata aatgaggtgg attcaatgaa aaaatttgta tctggaatat tagtaggaag
     8101 tctggcaaca gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga
     8161 tccaattgac gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg
     8221 cgtttctcgt taataattat ttaaaaatac gcatgaagac tttctaaaaa acagataagt
     8281 catcatgcgt atttttattt gtcagtcaat ataagttgtg ctattttatc tgcctgtctc
     8341 agattccatg gatctgtata aggtctagct actgctgcag ttagccaatc cacaccttca
     8401 acaggtattc catagcaata aaagtcagac tggatttccc ctgaagcatc aatcacgcga
     8461 ttcgtacctc gctcaacatc tatagcttct gaatgataac ttcctgtatt atctgaaaat
     8521 gaaaaagggc gaacaagttt gttttccttc aaatttttta tgacaggatt acttgaacga
     8581 gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc gactgctgat ttctttatct
     8641 tgtagagaaa taacatagcg gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa
     8701 tgaaaaatac ccgcatctat cagtgctgct aattctttcg tccgaatgat tggcggaccg
     8761 atcgttaaaa agctatttag atgagtaaac catccccata gtatttcttt gtattcctta
     8821 gcagaaaaaa cgtgccattc aatagcctgc cggattggat cacgccagtc ttttaatgca
     8881 tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct
     8941 tcaataaata ttcggctagc ttctgaaaag ggctcattct gataggcaag aggagtttct
     9001 aaatacgacc aatcccatct ataagggatt agtgctggat gctttccgag acactcctct
     9061 gaagagaggg agagaaaatc atgctcaaaa tttttctttg gaatcggcaa gtgatattct
     9121 tcgatgattt tcttgtaata aaacaactca acgtcttttt tcagatagtc aaaaaatgtt
     9181 tcgccagtca aaaatcccga ctgatgccat tgctccaggt tttcttttgt tagtaaccgt
     9241 ggccatgcca ttgctccgcc ttgcttttga tttcttcctc gagggtaata agggagcccc
     9301 cttcttgatc cactgtatac aatcggttcc ttgcctgatg gatgatagac cagttttccc
     9361 ttttcgttct cgaagcgccc tcctcttgat actgtaaaca atccgacata atcaaaaaaa
     9421 cttaagccta gacctcttaa aaagacattt tctttaggcg gtatcttcga aagatctaca
     9481 tctgcaggat tagaaggaag ctgatacagt aaaacatttt tctttgcgta gtctacccac
     9541 actctttctt cttctgtctc atcattttcc cagtgaccgc ttgctaaaac aagctgatcg
     9601 atagaatagc tttgctgatc tgtctgtaaa agataaccat cttttctttt gcttactttt
     9661 tttactaaag aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa
     9721 aaccagcgtt gatagagtcc gtataaacat cgagtagatt gctgatcttt ttccagacga
     9781 ttgatttctt ctataattag ttgcttattt ttggttaccg tctgttcgat ataatctgta
     9841 gcaggtcctt tactccattg aaacaagttg ggtccctgtg ttaccaatcc tccactggac
     9901 aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa taactccggt
     9961 ggctgattca accgccaaac tcgacctcca ggaccattgg gatcgaaaag aaaaagttca
    10021 agagaatgtc tgtcctgatt attatggaga atcctttctg ctacacttaa tcctctaggt
    10081 ccggcaccaa tgatccctat tttcatttgc atgcctccat ttcgataaat tttttcaata
    10141 aaacagcatg gttctgatgt tcattttttg caccataaac aaaagtaatg actggatgtt
    10201 ctttcataag ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt
    10261 cttgctggta tttctttgca aatgaagcaa atcgttctgg tacatggtca aaagttttgc
    10321 gtgcttcatt actaggtgca atttctttca accacagatc taactgcgct ttttcttttg
    10381 aaattcctcg tggccagaat ctatccacga gtattctata gccatcctct ttttcagcat
    10441 caaggtaaac acgttttaat ttgatcatag ttgtttcctt ctttctacct tttcgcttag
    10501 cttatatatg tagataatag cacaaaataa aaaaaatgaa cggcacaact atccattgcg
    10561 ccgttcatgt gatcttggtc aaaatctaat gattgattcg gtttttaaag ctgtcataca
    10621 atcatctgtt ttatactttt ttacttttga tttttccaga ccatccgtca tatccaccct
    10681 taagtaaata aatctcttga taacctgctt tacgtaattt atttgctgta cggatgctca
    10741 aacttttctt ttgatcgtaa atatagacag gctggtcttt tcgaatagat cctaatgaat
    10801 ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat atgcccagca tcaaacacat
    10861 ctttttcgcg aacatcgatt acttgtgctt ttctcattcc ttctcgaaat tcttcttctg
    10921 tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata gacttcccaa aggatgattg
    10981 caagcacaat caatagcaaa atgatattaa ttacccataa aaccatgtaa tgcctccgtt
    11041 tttttaagca aatttcaagg ctaatgaggc agcaccaatg acaccagctt cattaccaag
    11101 ctctgctaat ttgatttttg ttgattctct tacttgcggg aaagtaaatt ctttaaaata
    11161 tttctctaca cggctacgta aaaattctcc tgctgccgat actcctccac ctaacacgat
    11221 acttgacgga tttaatgtat tccccaagtt tccgcaagct aaacctaagt agaagcatac
    11281 gcgatcaaca accatcaatg caaacggatc gtcttcttga gccatttcaa agatatcttt
    11341 acttgttact tcttctccgt tgtctagcat tgctttcagt tttgaatccc ctgcgtattc
    11401 ttctgccaag aaacgaccta ggcgtaccac acctgtagcg ctagatacag tttctaaaca
    11461 tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc acagtgatat gtccgatttc
    11521 tcctccagct ccgctcacgc cgtgaatcaa atttccgcca gcaacgatac ctccgccaac
    11581 acctgttcct agagtaacga atacaacgtc tggatcgttt tctccagcac ctttccatcg
    11641 ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc
    11701 ctcgatcagt tttttcacaa attgaacttc tgtccagttt aagttatacg caccaataac
    11761 cgttccagct tcgctgtcca cgcttcctgg ggtacccatg ccgattccga taaagtcttc
    11821 ggctttcata ttatataaat tcaaacgatg attgattgat tcaatgattt caggaacgat
    11881 atgcttacca tcatccaaaa tattggtatc gatgctccat ttttgctgaa tctctccttc
    11941 tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa tcaatcccaa taatcttctt
    12001 gtccattaca aatttccttt ctttcggctt tcttcaattc ggtgctctct ttttaatatg
    12061 cgccacgctg tcatgtatgt atcatgatca atcaaacggc tatcatacag attacgcaat
    12121 tcgatagtca ttaattcaat gtcatagatg cgtgcaccca catagataaa gatgccaaat
    12181 tttttgaaca gctgctgtac atcatataag ctctccatct ttaagcactt ccttcatatt
    12241 atacaagtaa accatatttt tttaaaccca gtaaaataca tattacaaaa agaaatacaa
    12301 aaactagtgt agaaagaatc ctttggtgta tcgaatagct tgtctgttgt ttcggtagac
    12361 ccaaaacatt ccctaacact aatccgccaa ttagcccacc gacatgtccc caaacatcta
    12421 cagaggtatc cagaagacca aaaacaaatg tcaacacaat aaataaaaga aaatgtctga
    12481 ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc taagataaac aacgcaccga
    12541 acaaaccgaa tatcgaagta cttgcaccac cagacagaac attcgcttcg ttaaaagcaa
    12601 agcttgctgt atttcccatt atcccgctga acatataaat caagaaaaaa cgccaatggc
    12661 catagattgc ctcgatttgc tgccccataa aatacagtat cagtgagttc attacgaaat
    12721 gcatcagtcc aaaatggatg aagattggtg tcactaaacg ccaccattca ttgaagtgaa
    12781 caagataagg accatacatt ccggcataat aagggatctc aagtcgcgga aataattcca
    12841 tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata ggtccataac ggtcgtctta
    12901 accatgcacg caattttatt tgctgttgat aattcatgaa aacctcccat ttcattttag
    12961 tcaataaaca attgttggat cttctgatca aagctctccg gttgccattg ttcattcaat
    13021 tgttcacgaa aaacaaggct acatgttttt ttatgaaaat tatttaaata ccggtcgtaa
    13081 taaccaccgc caaaaccgat tcgataaccg gctctattgt agataatacc tggtacaatg
    13141 aggagatcga tcatctccgg caaatagagc ttctctgatt gtggctcaag aacaccaaaa
    13201 ctcgaacgta caagaacgga ctgttcatcc atttcataaa acgccatctg acgtttcggc
    13261 aaagttttag gtacaactat tttcttttgt tctttttctg ctcgttggat aaccggctgt
    13321 gtatccagtt caaattcatt cgataaagtc gtccctatca cttttgctga cttccagtac
    13381 tcgctggcga atagctgttt atagattcgc tgttcttttt cctttttctt gccacttttg
    13441 gccagatttt tcagcttttc gattcctttc cggcgtaatc ttactttcaa ttggtccaaa
    13501 aactcaccct tccttctctc ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac
    13561 tttcaaataa atgcataaat agaataaaaa gaagacctag aaatgattgg ccttcctttt
    13621 ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat
    13681 atggcgcacc aaaaagggca atcaaaattc ctgtaggtac ttccccactt ggtagaatca
    13741 ctctagcaac catatctgca ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa
    13801 aggtccagtt attttcttta cgtacgacta gcttcgcagc atgaggagct agtagtccta
    13861 caaaagataa actacctgtc accgctacac taccgcttgc caaagcaaca gctaacaaca
    13921 aaaggatttt ttgactttta gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa
    13981 atgatagcaa tacaatcctc tgtgcataaa gtcctcctag cggaattaag ataaccgccc
    14041 atggaagcaa tgcccaaaca taaggccagc tcgcacccca gattgatccc gctaaccatg
    14101 cattcacaaa tccataattt tccttagata attggatcgt gcatagcaat gttaatgcag
    14161 aaaggccggc attaacggca ataccggaca ataagattcg attcataccc aaaaagccat
    14221 ttcgatcata ggccgcaaga tagacacagc aagcagctaa aacaccacct agaaaggcta
    14281 caactagcaa tagaaacggc agcgaatagt gcgaaaagaa acctaaataa agcatcacta
    14341 aaaatcctgc tccggcatta atgcccaata tactagaatc tgccaattca tttcttgtta
    14401 ctccttgcaa gatataacca gaaacgccaa gacaagctcc tgctaataaa ctgatcagta
    14461 atcgaggaac tcgaaattct ctaacaatca gcgccaaaga agatgattct ttccctatga
    14521 acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc aatcattaaa ctacctatag
    14581 acaggatgac aagtacaaaa agcaagaaat agaacgtcca atatattttt ttcaccgttg
    14641 ttccctcctt acttgtatca gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa
    14701 aggtgtttca aaagggggat tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc
    14761 accgccaact aaaaaagaga caggtagaac ataacgatag tccgttccaa ccattccgcg
    14821 aacaactggc ggaaccaaca gcccaataaa tgaaacaggt ccgactaacg aaacggcaat
    14881 ggctgctaac actgcaacta gacagaaaac aatcaaccgc gttcgtttag gatgacttcc
    14941 aagagctaat gcatgtgttt cactcaaatg aatcaaagat attgcttttc ctaatgaaaa
    15001 agaaaccaaa acagcaagta tatataaagg aaacaatact gctaattgtt tccatgtgat
    15061 attggcagct ccgcctacaa accaaaaagc aagatcctgc tgcagattaa agaattgggt
    15121 aagtgcttgg ctgatagcag taaaaaaaga actaatagct acgccagcta aaatcagctg
    15181 taaaggattc atgcccattt tcgaaaactg caaaaaacca aatacgagca aagttacagc
    15241 aaaagaacct gccaatgaca ttagaaacac agaaaaatca gaagctttag gccaaaaagc
    15301 aaaactaagg gccattgcca acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc
    15361 taaaggattt cttgtcaccc cttgcatcaa tgcaccgctc aatgcaaaac aactccctac
    15421 aagaaatgct cccgccacac gaggcaaacg aatatttcgt atgatttggt gactttgttt
    15481 tgatctatca aaatcaaaca acgcctgcca aaaatctgct ttttccacag tcgctgcgcc
    15541 atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct
    15601 atttttcttc atagtcctca taattgatcc tcctgctctt ttggcaacaa atcataggtt
    15661 agaaaaactg gtttatttgt tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt
    15721 tttttcatac actcttttgt gatcacttct tcaggtgtac cttctgtgat caacttaccg
    15781 tttttcattg ctaataaata atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac
    15841 atcaagatcg ttgtttgttt catttgattg atttcagtca ataactgtag gatctctaat
    15901 tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga tcaagatatc tgtttcctga
    15961 gcaagcgcca tagcgatcca gacacgctgg gtttgtccac cagacagcgt tgcgatatcc
    16021 cgcatacgaa atccagctag acctgtagca tccaacgccc actcaattgc ttcatgatct
    16081 tgcgtgtcca tgccactgaa cagtttttga taagggtatc gtccgtaaga tactacttct
    16141 tctactgtca tacctgtagg gtgttcacta gtttgtgcta ataatgcaat tttttttgca
    16201 agttcttttg aactgtatgt agagatatca gagttattga caatgatctt tcctgatttc
    16261 acaggtagta tacgggtaaa acttttcaat aaagtagatt ttccacttcc atttggccca
    16321 atcaaactag tgattttccc ttcaggaatc gtaacagaca tgttttcaat gatttttttg
    16381 ttttcgtagc cggctgcaat gtcagctgct cttaatctcg tcatttcacc catcctttcg
    16441 ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc gtcaagcgct actttcatat
    16501 ctatagccaa atgaagcggt tgacagcgtt cgaaatatca ggaataataa ccaatgagaa
    16561 agattctcaa ttatgttagg aggaaagaaa tgaaattaaa aacaaaagta ttcacattat
    16621 ctagcatatt gctgctggct ggagtactga gcgcttgctc cccctcttca gataaagaaa
    16681 cagctacaac tgccaattcg acgacttcat ccaatagtac tcatacagtc acagatacac
    16741 ttggacataa agtagagatt ccaaacgcgc ccaaacggat catcggcagt tacttagaag
    16801 actatctgat tgcactaggg gaaaaaccta tcgcccagtg gactgttggc agtggttcca
    16861 ttcaagatta tctgcaagat gatttaaagg atgtcccaac gatttcttat gaccttcctt
    16921 atgagaaagt gttaagcttt gagcctgatt tgctgttgat ttcttcttcc gctactgtcg
    16981 aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa
    17041 cagatgtcac atgggaagaa caactaaaag atgtcggcaa agctttggca aaagaaaaag
    17101 aggcagaaaa aatcatttcc gattatcaaa aatccatcaa gaaaactcga gaagaactag
    17161 cagataaaat cgagaacaag acagctgcag tgttgtgggt gaccaacaac tcagccttta
    17221 tggtcagcaa aaatcggtca agtggaagaa tcgtatacga cgatctggaa tttggtgttc
    17281 ctgatctagt ggaagaggtc tccaaaaaag ccacttctga ctggtctgct gtttcctctg
    17341 aaaagctagc tgaattagat gctgactaca tcatccttgt gaacagtgac aaagatgctg
    17401 ccatgttcaa tgaaccaagc tggaagaatc taaaagcagt gacagacaat catgtactgc
    17461 aattcggacc tgaatctagt tggctatata acggacctat tgccagtcag gaaatggtca
    17521 atgatatcaa agaaaaacta aattgatttt aactaaaaaa agcatcttca cgattagtcg
    17581 tgagatgctt tttttcttat taacaaacta gacgcataga ttttattctt cttttgaagc
    17641 aaccctattt tttactcttt cccatttttg aaaacgatgg gcatatagat tttcttgatc
    17701 cgttatcccc tcatactcct ctataagggc gaagtctgaa aaatcaatat tcccaatata
    17761 cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag atccgaccag tgtaaggaag
    17821 aagttcttga aaaatccgac taccacccga aacatagata tcttctgaga tcgtttgact
    17881 atacgacaaa agttcatcga tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt
    17941 tggttgcgtt gtcaacacga tgatatggcg ataaggaagt gaaagatccc ccattccttc
    18001 ataggtcttt cttcccatca ctaaaattcg atccattgta tgttcgcgaa agaaacgcat
    18061 atcatttggc aatcgccatg gtaacaaacc atttttgccg atcagaccat ttttatcttg
    18121 tgcccacatg gaaataaaca tattatccgc tccttatgat tttagatgtt ttgagataaa
    18181 aaaagtctca attctttgac cagcaaggaa ttgagaactt taaaaacatt attttgtttc
    18241 acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg
    18301 attgttacgt ttgtttttgc ttgttagata gttacgttct ttacaagaag tacactctaa
    18361 agtgatgttt acgcgcatgt ttttccctcc catttttaca agaatcctct aagatgactc
    18421 tcagacttga ttatcttacc atgatttttt ctagattgct agtgtttttc acgattttgt
    18481 taagtaaaac tacttgacga taaatacaac ggttttactt ctcaaacaaa atctttttct
    18541 ttttgtataa atacccttac acttgtttat atagattatg ttttttaata aagggggata
    18601 ctaatatgcc atggaacatg aaggactttc ctgcatcaat gaaaaactta gataaattga
    18661 caagaaaaaa agcaatcgac atcgcaaatg cattattaga tgaagggtac cctgataacc
    18721 gtgcgatccc aattgccatt gatcaagcaa aaaaatggta tgaagatgct agtgaatcag
    18781 aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac ggatgaacac gatacgaatc
    18841 ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga atatgaggaa gaacaatgga
    18901 tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa
    18961 tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac tttgttcatt tataaaaaag
    19021 atgggacaaa agaaaaagaa atcgattatt gaacatatgt aaaggggttg tgacgaaaat
    19081 cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa
    19141 gtcataatat ctataaaacg ggaagagctg ttttttgaaa ttgtttctta tcactcagtg
    19201 ttaaacggta ggagaaaacc tcttttttat tgatatttga ttttcatcaa acagtcacat
    19261 agtcgattgc ttctgcttgc ggaatcgttt catctacaaa gtagacttta taccatgtca
    19321 agaaactgaa gattgtccag atttttcttt gctcttcttt acgtccttcg tgatggtcat
    19381 ctagcaactt catgattttt tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata
    19441 atgctttgac ttgttcgtag ccgtgttctt ctttcaacca agctttgatt ggcacaggga
    19501 atcctaattt cacacgattc gaccattctt ccggaagatg tttgttcgca gcttgtctga
    19561 aaatatcttt tgtgttgttt tgattcaata aaaacttact tggtatttgt tgagccaatt
    19621 tcatgacttc gatatccaat aaaggtactc ggacttccaa agaacttgcc atagaaagtt
    19681 tatctgcctt taacaagata tcttttggca tccattgatg aagatcgaca tattgcatct
    19741 tattgacttc atctttgatt ccttctgttt tctcgtaatg aacagtcatg atttcatcca
    19801 cagatggtgc tgtttgatat tttggtgtta acacttctga tgcttctgat tcttcgaaca
    19861 ctcgagcatg accgataaaa tattcttttg caggagcaag tgattcatat aaatggatcc
    19921 gtccatggaa gtttttcatt tttccgattt tacgtcctaa attatagcgt gttccttttg
    19981 gtaatttttt caatccttga gcaatcacac ggatgaattt tgaattcgta tggaatccat
    20041 acgtttggta tcccgcaaac agttcatccg ccccttctcc agattggact acccgcacac
    20101 tttgtgaagc aagatttgct aaaaagtaca aaggtacgca agaaggattt gaatcaggtt
    20161 catctaagtg atactggatc aaagggaatg ctttgaacga catgtctccg tcgatcactg
    20221 ctgctgtatt atctaagtcc aatagttcgg ttagttttct ggcttcaatc gcttcattat
    20281 aggttttatc atcaaacccg attgagaaag aatgatctgg tttcaataca gaggttacat
    20341 aactggaatc aaccccgctg gataagaaag aaccaacttc tacatcactg accgtatgtg
    20401 cttcaataga agcttgaacg gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg
    20461 tttcttttgt ttcaaaatct gcatcccaat attgctgaat gtctattttt ccatctttat
    20521 acgtataata atggccttca ggcaaacgat agacatcttt gaagaatgtt tcgccgttta
    20581 atggagaata ttggaaagtc atataaggtt tcaacgcttc tttgttcaat tctttgttaa
    20641 aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa cataaatgtt ccgttcattt
    20701 ctgcataata atatggtttg atcccaaagt ggtctcttgc accgaataac tcattctttt
    20761 cattatccca aatagcaaaa gcaaacattc cgcggatctt ttgcaataat tctgttcccc
    20821 attcttcgta accatgcaaa aggacttctg tatctgcatg tgtttgaaaa acgtggccag
    20881 ctgcaatcaa ttcttctctc aaaggctggt aattatagat ttcgccatta aaaataatga
    20941 tctttgtccg atcttcattg taaataggct gagtaccgcc ctctaagtca ataatactta
    21001 atcttctgaa tcctaaagca acatgtttat cgatatattc tcctgaacta ttaggtccac
    21061 ggtgaacgat tcgatccatc attgtattaa taattgtttt tttgttgtcc ttgtcgttaa
    21121 caaaacctac aataccgcac atttaacttt cacatcctaa tataaaatta ctgtttattt
    21181 cattcatatg catcttatca tagaataaat agcggacgaa tattttctct cttacaattt
    21241 cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca gaaaataaag tcccaatatc
    21301 taatttacca tgttttcttt tttgaggcta ccttattctc ttcgtgttaa aaaaatatta
    21361 gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg acctaaagat cgtgt
//
LOCUS       DAJSBC010000043        20706 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSBC010000043 DAJSBC010000000
VERSION     DAJSBC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.043"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT65_001744"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT65_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR2329357.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="ONT65_001745"
     CDS             complement(3004..3438)
                     /locus_tag="ONT65_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329358.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..4660)
                     /locus_tag="ONT65_001746"
     CDS             complement(3455..4660)
                     /locus_tag="ONT65_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR2329359.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5354..5740
                     /locus_tag="ONT65_001747"
     CDS             5354..5740
                     /locus_tag="ONT65_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2329360.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5834..6025
                     /locus_tag="ONT65_001748"
     CDS             5834..6025
                     /locus_tag="ONT65_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329361.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6539..6871
                     /locus_tag="ONT65_001749"
     CDS             6539..6871
                     /locus_tag="ONT65_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2329362.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7167..7322
                     /locus_tag="ONT65_001750"
     CDS             7167..7322
                     /locus_tag="ONT65_001750"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR2329363.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7598..8938
                     /locus_tag="ONT65_001751"
                     /pseudo
     CDS             7598..8938
                     /locus_tag="ONT65_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9179..10795)
                     /locus_tag="ONT65_001752"
     CDS             complement(9179..10795)
                     /locus_tag="ONT65_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2329364.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10913..11131
                     /locus_tag="ONT65_001753"
     CDS             10913..11131
                     /locus_tag="ONT65_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329365.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11326..11787
                     /locus_tag="ONT65_001754"
     CDS             11326..11787
                     /locus_tag="ONT65_001754"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2329366.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11980..12219)
                     /locus_tag="ONT65_001755"
     CDS             complement(11980..12219)
                     /locus_tag="ONT65_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329367.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12243..13766)
                     /locus_tag="ONT65_001756"
     CDS             complement(12243..13766)
                     /locus_tag="ONT65_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2329368.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13784..13906)
                     /locus_tag="ONT65_001757"
     CDS             complement(13784..13906)
                     /locus_tag="ONT65_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2329369.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13936..14919)
                     /locus_tag="ONT65_001758"
     CDS             complement(13936..14919)
                     /locus_tag="ONT65_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2329370.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="ONT65_001759"
     CDS             complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="ONT65_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2329371.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15113..15490)
                     /locus_tag="ONT65_001760"
     CDS             complement(15113..15490)
                     /locus_tag="ONT65_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329372.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="ONT65_001761"
     CDS             complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="ONT65_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2329373.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="ONT65_001762"
     CDS             complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="ONT65_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2329374.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16508..18274)
                     /locus_tag="ONT65_001763"
     CDS             complement(16508..18274)
                     /locus_tag="ONT65_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329375.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18234..19988)
                     /locus_tag="ONT65_001764"
     CDS             complement(18234..19988)
                     /locus_tag="ONT65_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329376.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20098..20667)
                     /locus_tag="ONT65_001765"
     CDS             complement(20098..20667)
                     /locus_tag="ONT65_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR2329377.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         6651 a         3923 c         3063 g         7069 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4501 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4561 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4621 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4681 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4741 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4801 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4861 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4921 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4981 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     5041 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5101 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5161 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5221 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5281 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5341 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5401 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5461 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5521 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5581 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5641 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5701 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5761 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5821 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5881 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5941 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     6001 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6061 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6121 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6181 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6241 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6301 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6361 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6421 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6481 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6541 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6601 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6661 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6721 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6781 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6841 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6901 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6961 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     7021 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7081 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7141 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7201 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7261 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7321 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7381 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7441 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7501 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7561 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7621 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7681 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7741 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7801 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7861 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7921 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7981 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     8041 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8101 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8161 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8221 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8281 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8341 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8401 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8461 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8521 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8581 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8641 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8701 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8761 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8821 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8881 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8941 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     9001 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9061 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9181 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9421 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9481 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9601 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9781 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9841 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9901 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9961 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
    10021 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10081 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10141 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10201 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10261 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10321 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10381 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10441 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10501 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10561 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10621 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10681 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10741 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10801 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10861 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10921 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10981 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    11041 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11101 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11161 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11221 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11281 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11341 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11401 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11461 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11521 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11581 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11641 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11701 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11761 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11821 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11881 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11941 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    12001 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12061 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12121 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12181 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12241 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12301 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12361 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12421 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12481 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12541 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12601 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12661 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12721 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12781 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12841 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12901 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12961 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    13021 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13081 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13141 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13201 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13261 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13321 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13381 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13441 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13501 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13561 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13621 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13681 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13741 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13801 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13861 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13921 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13981 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    14041 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14101 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14161 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14221 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14281 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14341 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14401 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14461 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14521 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14581 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14641 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14701 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14761 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14821 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14881 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14941 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    15001 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15061 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15121 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15181 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15241 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15301 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15361 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15421 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15481 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15541 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15601 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15661 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15721 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15781 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15841 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15901 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15961 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    16021 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16081 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16141 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16201 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16261 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16321 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16381 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16441 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16501 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16561 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16621 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16681 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16741 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16801 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16861 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16921 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16981 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    17041 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17101 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17161 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17221 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17281 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17341 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17401 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17461 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17521 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17581 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17641 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17701 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17761 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17821 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17881 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17941 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    18001 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18061 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18121 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18181 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18241 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18301 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18361 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18421 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18481 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18541 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18601 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18661 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18721 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18781 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18841 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18901 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18961 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    19021 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19081 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19141 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19201 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19261 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19321 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19381 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19441 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19501 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19561 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19621 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19681 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19741 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    19801 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    19861 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    19921 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    19981 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    20041 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    20101 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    20161 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    20221 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    20281 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    20341 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    20401 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    20461 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    20521 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    20581 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    20641 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    20701 ttaaaa
//
LOCUS       DAJSBC010000044        20585 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSBC010000044 DAJSBC010000000
VERSION     DAJSBC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.044"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            133..3660
                     /locus_tag="ONT65_001766"
     CDS             133..3660
                     /locus_tag="ONT65_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR2329378.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ONT65_001767"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ONT65_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR2329379.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ONT65_001768"
     CDS             7448..7789
                     /locus_tag="ONT65_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2329380.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ONT65_001769"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ONT65_001769"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR2329381.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ONT65_001770"
     CDS             9146..11566
                     /locus_tag="ONT65_001770"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR2329382.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT65_001771"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT65_001771"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR2329383.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ONT65_001772"
     CDS             13000..13737
                     /locus_tag="ONT65_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2329384.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ONT65_001773"
     CDS             13751..14566
                     /locus_tag="ONT65_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2329385.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ONT65_001774"
     CDS             14579..15226
                     /locus_tag="ONT65_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2329386.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ONT65_001775"
     CDS             15242..15898
                     /locus_tag="ONT65_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2329387.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ONT65_001776"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ONT65_001776"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR2329388.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ONT65_001777"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ONT65_001777"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR2329389.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ONT65_001778"
     CDS             18240..18758
                     /locus_tag="ONT65_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2329390.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ONT65_001779"
     CDS             18865..19365
                     /locus_tag="ONT65_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2329391.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ONT65_001780"
     CDS             complement(19645..20565)
                     /locus_tag="ONT65_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR2329392.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6889 a         3571 c         4391 g         5734 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaat
//
LOCUS       DAJSBC010000045        20488 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSBC010000045 DAJSBC010000000
VERSION     DAJSBC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.045"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ONT65_001781"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ONT65_001781"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR2329393.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ONT65_001782"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ONT65_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR2329394.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ONT65_001783"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ONT65_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR2329395.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ONT65_001784"
     CDS             2857..3732
                     /locus_tag="ONT65_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR2329396.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT65_001785"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT65_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR2329397.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ONT65_001786"
     CDS             4629..5063
                     /locus_tag="ONT65_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR2329398.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ONT65_001787"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ONT65_001787"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR2329399.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ONT65_001788"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ONT65_001788"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR2329400.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ONT65_001789"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ONT65_001789"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR2329401.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ONT65_001790"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ONT65_001790"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR2329402.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ONT65_001791"
     CDS             13334..14260
                     /locus_tag="ONT65_001791"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR2329403.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ONT65_001792"
     CDS             14442..15335
                     /locus_tag="ONT65_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR2329404.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ONT65_001793"
     CDS             15469..15594
                     /locus_tag="ONT65_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2329405.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ONT65_001794"
     CDS             complement(15728..16360)
                     /locus_tag="ONT65_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329406.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ONT65_001795"
     CDS             complement(16508..17050)
                     /locus_tag="ONT65_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR2329407.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ONT65_001796"
     CDS             17185..17574
                     /locus_tag="ONT65_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329408.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ONT65_001797"
     CDS             17578..18342
                     /locus_tag="ONT65_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2329409.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ONT65_001798"
     CDS             18471..19418
                     /locus_tag="ONT65_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2329410.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ONT65_001799"
     CDS             19548..20288
                     /locus_tag="ONT65_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329411.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7194 a         3481 c         4316 g         5497 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtttat
//
LOCUS       DAJSBC010000046        20412 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSBC010000046 DAJSBC010000000
VERSION     DAJSBC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.046"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..1091
                     /locus_tag="ONT65_001800"
     CDS             12..1091
                     /locus_tag="ONT65_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2329412.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1288..1989)
                     /locus_tag="ONT65_001801"
                     /pseudo
     CDS             complement(1288..1989)
                     /locus_tag="ONT65_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2003..2927)
                     /locus_tag="ONT65_001802"
                     /pseudo
     CDS             complement(2003..2927)
                     /locus_tag="ONT65_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3044..4123
                     /locus_tag="ONT65_001803"
     CDS             3044..4123
                     /locus_tag="ONT65_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2329413.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4155..4748
                     /locus_tag="ONT65_001804"
     CDS             4155..4748
                     /locus_tag="ONT65_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2329414.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4858..5085)
                     /locus_tag="ONT65_001805"
     CDS             complement(4858..5085)
                     /locus_tag="ONT65_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2329415.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5188..7776)
                     /locus_tag="ONT65_001806"
     CDS             complement(5188..7776)
                     /locus_tag="ONT65_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR2329416.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7796..8455)
                     /locus_tag="ONT65_001807"
     CDS             complement(7796..8455)
                     /locus_tag="ONT65_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2329417.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8504..9007)
                     /gene="trmL"
                     /locus_tag="ONT65_001808"
     CDS             complement(8504..9007)
                     /gene="trmL"
                     /locus_tag="ONT65_001808"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR2329418.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9228..10094)
                     /locus_tag="ONT65_001809"
     CDS             complement(9228..10094)
                     /locus_tag="ONT65_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2329419.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10203..10844
                     /locus_tag="ONT65_001810"
     CDS             10203..10844
                     /locus_tag="ONT65_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR2329420.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10906..12267)
                     /gene="mgtE"
                     /locus_tag="ONT65_001811"
     CDS             complement(10906..12267)
                     /gene="mgtE"
                     /locus_tag="ONT65_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR2329421.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12285..13184)
                     /locus_tag="ONT65_001812"
     CDS             complement(12285..13184)
                     /locus_tag="ONT65_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2329422.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13186..13983)
                     /locus_tag="ONT65_001813"
     CDS             complement(13186..13983)
                     /locus_tag="ONT65_001813"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR2329423.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13961..14665)
                     /locus_tag="ONT65_001814"
     CDS             complement(13961..14665)
                     /locus_tag="ONT65_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR2329424.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14796..15371
                     /locus_tag="ONT65_001815"
     CDS             14796..15371
                     /locus_tag="ONT65_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR2329425.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15489..16166
                     /locus_tag="ONT65_001816"
     CDS             15489..16166
                     /locus_tag="ONT65_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2329426.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16245..18053)
                     /gene="pepF"
                     /locus_tag="ONT65_001817"
     CDS             complement(16245..18053)
                     /gene="pepF"
                     /locus_tag="ONT65_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR2329427.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18229..19227)
                     /locus_tag="ONT65_001818"
     CDS             complement(18229..19227)
                     /locus_tag="ONT65_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR2329428.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19453..20106)
                     /locus_tag="ONT65_001819"
     CDS             complement(19453..20106)
                     /locus_tag="ONT65_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR2329429.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5907 a         4440 c         3638 g         6427 t
ORIGIN      
        1 gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga aaacatcttt
       61 cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc aaccgtcagg
      121 ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat cgcggaacca
      181 tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac tattctgttt
      241 atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt ggcagacgcc
      301 ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg ttaaatgaaa
      361 aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc gattgtgcca
      421 tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg cgaacaaaga
      481 acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca ggacaccgtc
      541 ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt gaaacacggg
      601 aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag tcggatgccg
      661 tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag ttgaacggga
      721 aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct ggcgctaact
      781 tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct gggctacacg
      841 atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct tgggagcgtg
      901 ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga agcccgatta
      961 gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat tacccgcgga
     1021 aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag caagaacggt
     1081 tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg cttcatgtct
     1141 ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt cattatcaag
     1201 gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt tacctgtagt
     1261 ggctaactta aacttgaaat ttacggacgt aaagcttgct gccaaattag cgattgaaac
     1321 taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt gatttgcttg
     1381 ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca ttccaatcag
     1441 agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt cttgaaaaga
     1501 tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca cttctgccac
     1561 tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt tcatcaacgt
     1621 ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa tgagataaaa
     1681 accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata gttcctgtac
     1741 ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa aattttccga
     1801 aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga gattgccacc
     1861 ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg gaaaggccaa
     1921 tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt tataaaaatg
     1981 ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc tctaaaaaga
     2041 catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt tcttcttgta
     2101 agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact tggagctgac
     2161 cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt ttctgggagt
     2221 aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag agattctttg
     2281 gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca aagaatttct
     2341 acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt cagctccttg
     2401 atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg ctcatctaaa
     2461 aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg tttcaagcca
     2521 ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa ttgaatcacc
     2581 tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca aaaataatcg
     2641 atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc
     2701 attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt aatggttcct
     2761 ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc actgccgttt
     2821 ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg attcaatgct
     2881 acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat gaaaaactcc
     2941 ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact agtgacgatt
     3001 ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata aatctcggct
     3061 actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa gtcaaggatt
     3121 cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg tcagttattt
     3181 actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat gtgccttttt
     3241 acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg aagaaagcca
     3301 ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta atcatccccc
     3361 catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac gaaaaaaaga
     3421 tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc attgggaaaa
     3481 acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa cacaattagc
     3541 cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca atgtcggtca
     3601 tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa
     3661 actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg atcggattcg
     3721 tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct tagagtttct
     3781 gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag gattgaatga
     3841 aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca gtatcaaaca
     3901 tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt atcgcttgca
     3961 aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg gtttgaaaac
     4021 gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag aaaaccagca
     4081 atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt agtcattatt
     4141 gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg aaagagatcc
     4201 gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa acccatcgtc
     4261 ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat gctgccaaag
     4321 aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc aaagatgaag
     4381 aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa cagaacttgc
     4441 aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt ggtaatgaaa
     4501 tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt catgcttatg
     4561 cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg aaaaacaata
     4621 aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac atcagccaat
     4681 tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt tatcagcatt
     4741 tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt
     4801 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
     4861 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
     4921 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
     4981 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
     5041 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
     5101 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
     5161 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
     5221 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
     5281 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
     5341 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
     5401 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
     5461 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
     5521 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
     5581 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
     5641 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
     5701 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
     5761 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     5821 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     5881 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     5941 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     6001 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     6061 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     6121 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     6181 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     6241 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     6301 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     6361 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     6421 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     6481 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     6541 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     6601 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     6661 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     6721 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     6781 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     6841 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     6901 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     6961 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     7021 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     7081 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     7141 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     7201 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     7261 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     7321 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     7381 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     7441 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     7501 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     7561 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     7621 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     7681 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     7741 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     7801 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     7861 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     7921 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     7981 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg gcaaccgcat
     8041 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     8101 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     8161 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     8221 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     8281 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     8341 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     8401 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     8461 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     8521 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     8581 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     8641 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     8701 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     8761 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     8821 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     8881 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     8941 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     9001 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     9061 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     9121 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     9181 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     9241 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     9301 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     9361 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     9421 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     9481 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     9541 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     9601 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     9661 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     9721 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     9781 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     9841 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     9901 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     9961 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
    10021 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
    10081 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
    10141 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
    10201 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
    10261 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
    10321 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
    10381 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
    10441 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
    10501 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
    10561 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
    10621 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
    10681 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
    10741 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
    10801 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
    10861 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
    10921 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
    10981 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
    11041 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
    11101 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
    11161 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
    11221 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
    11281 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
    11341 gtcttcatag tgactgatta aagaagcggt cgacataccc aaaaacaaca gagtgatcag
    11401 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
    11461 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
    11521 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
    11581 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
    11641 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
    11701 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
    11761 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
    11821 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
    11881 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
    11941 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
    12001 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
    12061 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
    12121 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
    12181 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
    12241 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
    12301 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
    12361 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
    12421 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
    12481 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
    12541 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
    12601 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
    12661 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
    12721 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc agatgtatcc
    12781 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
    12841 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
    12901 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
    12961 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
    13021 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
    13081 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
    13141 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
    13201 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
    13261 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
    13321 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
    13381 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
    13441 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
    13501 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
    13561 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
    13621 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
    13681 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
    13741 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
    13801 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
    13861 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
    13921 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
    13981 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
    14041 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
    14101 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
    14161 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
    14221 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
    14281 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
    14341 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
    14401 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
    14461 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
    14521 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
    14581 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
    14641 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
    14701 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
    14761 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    14821 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    14881 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    14941 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    15001 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    15061 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    15121 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgggctgaat
    15181 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    15241 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    15301 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    15361 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    15421 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    15481 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    15541 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    15601 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    15661 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    15721 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    15781 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    15841 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    15901 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    15961 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    16021 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    16081 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    16141 gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa gagagccgac
    16201 gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa acgatcgatc
    16261 aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc aatatatgca
    16321 gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc gctgttacct
    16381 gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag tattttcttc
    16441 gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta attataataa
    16501 aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac tgctggacca
    16561 taatattttg cattcaattt accataacta tcacttaggt attcactagt caatggcaca
    16621 cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt ctggcggaaa
    16681 actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac acgagggtct
    16741 ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga agcaatttct
    16801 gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt gaaataacta
    16861 tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt atcatgccaa
    16921 ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcacc gcttcgtttc
    16981 cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac gatggccatg
    17041 tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc ttcgtacgta
    17101 aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg cagtttttcc
    17161 acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa atgcttgttt
    17221 accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct caaagacgct
    17281 tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt tatatgtgtg
    17341 cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa tcccttaaat
    17401 gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac gccatgagat
    17461 aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc tgcattattc
    17521 aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc aagaagagat
    17581 tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat ataatggcga
    17641 tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc tgacaattgc
    17701 aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag actgcttgct
    17761 cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt aaggtgcgca
    17821 tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat cgcatttaaa
    17881 aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt taactcttct
    17941 gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt ggtcaagtcc
    18001 caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc catgaaatcc
    18061 actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac atccacatgt
    18121 cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt agcataacta
    18181 aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact attttttcaa
    18241 agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt catcaatcat
    18301 aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt tgaaatcagg
    18361 aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa cctgttcttg
    18421 taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca aaagatgttt
    18481 atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt gaataagttg
    18541 gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca tttggttcat
    18601 tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag tccagcagct
    18661 actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca aataaagctt
    18721 ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat agtagcagca
    18781 acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa gtatccacca
    18841 tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct ggtgagacag
    18901 tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc tttgccgcaa
    18961 cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc aatccatgaa
    19021 gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg aacagttatc
    19081 tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa tcactctttt
    19141 tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa aacagcactt
    19201 attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta cgtctaagaa
    19261 tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta ataagaacag
    19321 ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg cagatgcaag
    19381 cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac agtctcttac
    19441 tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac gcgccatcag
    19501 acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag caaactccaa
    19561 aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc agaagtgaac
    19621 gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact ctagttcgac
    19681 atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg cttgtctttc
    19741 ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa taatctgctg
    19801 acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt gagaaatttc
    19861 ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg gcaaaacttg
    19921 gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt ccaaaatact
    19981 ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg aaacgccccg
    20041 ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa tatgttccat
    20101 ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt gtagcgaaat
    20161 cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa accagcggat
    20221 ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg aatactatat
    20281 agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat cttctactac
    20341 ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg gaaaaactat
    20401 ctttgtaata gt
//
LOCUS       DAJSBC010000047        19655 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSBC010000047 DAJSBC010000000
VERSION     DAJSBC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.047"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            29..676
                     /locus_tag="ONT65_001820"
     CDS             29..676
                     /locus_tag="ONT65_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2329430.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            760..1704
                     /locus_tag="ONT65_001821"
     CDS             760..1704
                     /locus_tag="ONT65_001821"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR2329431.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1839..3290
                     /locus_tag="ONT65_001822"
     CDS             1839..3290
                     /locus_tag="ONT65_001822"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR2329432.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3311..4147
                     /locus_tag="ONT65_001823"
     CDS             3311..4147
                     /locus_tag="ONT65_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2329433.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4147..4806
                     /locus_tag="ONT65_001824"
     CDS             4147..4806
                     /locus_tag="ONT65_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2329434.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4932..5621
                     /locus_tag="ONT65_001825"
     CDS             4932..5621
                     /locus_tag="ONT65_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329435.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5627..6583
                     /locus_tag="ONT65_001826"
     CDS             5627..6583
                     /locus_tag="ONT65_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR2329436.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6627..7139
                     /locus_tag="ONT65_001827"
     CDS             6627..7139
                     /locus_tag="ONT65_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2329437.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7395..7943)
                     /locus_tag="ONT65_001828"
     CDS             complement(7395..7943)
                     /locus_tag="ONT65_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2329438.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8032..9213)
                     /locus_tag="ONT65_001829"
     CDS             complement(8032..9213)
                     /locus_tag="ONT65_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR2329439.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9449..10957)
                     /locus_tag="ONT65_001830"
     CDS             complement(9449..10957)
                     /locus_tag="ONT65_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR2329440.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11116..11307
                     /locus_tag="ONT65_001831"
     CDS             11116..11307
                     /locus_tag="ONT65_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329441.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11510..12427
                     /locus_tag="ONT65_001832"
     CDS             11510..12427
                     /locus_tag="ONT65_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR2329442.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12420..13493
                     /locus_tag="ONT65_001833"
     CDS             12420..13493
                     /locus_tag="ONT65_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329443.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13641..14159
                     /locus_tag="ONT65_001834"
     CDS             13641..14159
                     /locus_tag="ONT65_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329444.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14140..14532
                     /locus_tag="ONT65_001835"
     CDS             14140..14532
                     /locus_tag="ONT65_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR2329445.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14608..15543
                     /locus_tag="ONT65_001836"
     CDS             14608..15543
                     /locus_tag="ONT65_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR2329446.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15676..16455
                     /locus_tag="ONT65_001837"
     CDS             15676..16455
                     /locus_tag="ONT65_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2329447.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16448..16765
                     /locus_tag="ONT65_001838"
     CDS             16448..16765
                     /locus_tag="ONT65_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2329448.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17009..18619
                     /locus_tag="ONT65_001839"
     CDS             17009..18619
                     /locus_tag="ONT65_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2329449.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18776..19624
                     /locus_tag="ONT65_001840"
     CDS             18776..19624
                     /locus_tag="ONT65_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2329450.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6350 a         3515 c         3957 g         5833 t
ORIGIN      
        1 ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt
       61 tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac
      121 gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac
      181 atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt
      241 gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag
      301 tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca
      361 aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga
      421 aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga
      481 taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac
      541 ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa
      601 agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac
      661 tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga
      721 tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt
      781 gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc
      841 caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg
      901 atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc
      961 aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat
     1021 gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc
     1081 caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa
     1141 ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc
     1201 acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg
     1261 cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca
     1321 ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa
     1381 gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag
     1441 ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc
     1501 attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca
     1561 agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt ttctagaatc
     1621 ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa
     1681 ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt
     1741 tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga
     1801 acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag
     1861 caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa
     1921 aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta
     1981 attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc
     2041 ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat
     2101 ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc
     2161 tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag
     2221 tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg
     2281 gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg
     2341 gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag
     2401 ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg
     2461 gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt
     2521 ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt
     2581 tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac
     2641 ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc
     2701 cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa
     2761 cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac
     2821 ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca
     2881 tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat
     2941 acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc
     3001 aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt
     3061 tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg
     3121 ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc
     3181 ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct
     3241 atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag
     3301 gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt
     3361 aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa
     3421 aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga
     3481 tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa
     3541 tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag
     3601 agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc
     3661 cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc
     3721 aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag
     3781 ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa
     3841 tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta
     3901 tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt
     3961 ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa
     4021 gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga
     4081 aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag
     4141 tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta
     4201 ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatctg
     4261 gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt
     4321 cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat
     4381 tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc
     4441 agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag
     4501 tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg
     4561 ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa
     4621 ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata
     4681 ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc
     4741 atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat gcatcagtca
     4801 atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt
     4861 atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg
     4921 aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt
     4981 gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg
     5041 tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc
     5101 tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata
     5161 attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa
     5221 taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat
     5281 ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa
     5341 accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg
     5401 taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca gaaaatcaag
     5461 atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa
     5521 tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac
     5581 aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta
     5641 tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct
     5701 ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt
     5761 gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc cccaagtagg
     5821 acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt
     5881 cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc
     5941 gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc
     6001 aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga
     6061 aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga
     6121 taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg
     6181 attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc
     6241 gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt
     6301 ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa
     6361 aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga
     6421 attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc
     6481 acttatgcat catttggaac ttattagtca cctgctagat catccaaaag attggcttaa
     6541 atattattca aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga
     6601 aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag
     6661 aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc
     6721 aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag
     6781 aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaagcg
     6841 tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat
     6901 cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct
     6961 tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt
     7021 atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga
     7081 tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac
     7141 tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga
     7201 tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa
     7261 tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact
     7321 cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta tcctacgagt
     7381 attattcgat acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac
     7441 gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct
     7501 aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt
     7561 ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc
     7621 ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct
     7681 actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg
     7741 tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa
     7801 taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc
     7861 ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg
     7921 ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag
     7981 ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat attataccag
     8041 caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa
     8101 tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat
     8161 gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt
     8221 acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt
     8281 aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct
     8341 gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat
     8401 cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc
     8461 aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt
     8521 ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt
     8581 tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata
     8641 tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc
     8701 cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga
     8761 aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt
     8821 gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat
     8881 cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga
     8941 aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg
     9001 cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc
     9061 aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag
     9121 tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac
     9181 aaataaacca ataataccta ataaataact caaataatca ctcctattcc aaattccgtt
     9241 ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt
     9301 agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa
     9361 agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc
     9421 gttttcaaca tttataagta aactttttct attggtaata tagattttcg gaagggtaga
     9481 caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga
     9541 ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt
     9601 gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta
     9661 gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta
     9721 atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga
     9781 taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg
     9841 gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat
     9901 ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt
     9961 ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc
    10021 attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat
    10081 catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag
    10141 attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc
    10201 gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga
    10261 catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa
    10321 cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt
    10381 catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca ggagctgtca
    10441 ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga
    10501 cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt
    10561 ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga
    10621 taacgacact gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat
    10681 ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat
    10741 agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat
    10801 cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc ttcccaccaa
    10861 attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt
    10921 caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa
    10981 agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac
    11041 aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga tagtttttga
    11101 taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc
    11161 cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg
    11221 tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc
    11281 acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg
    11341 tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc
    11401 ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta
    11461 ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc
    11521 agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg atcaggatac
    11581 aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca
    11641 attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat
    11701 ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca
    11761 cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt
    11821 tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta
    11881 ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa
    11941 gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat
    12001 caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca
    12061 aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg
    12121 taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga
    12181 aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc
    12241 attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag
    12301 gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt
    12361 gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta
    12421 tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag
    12481 aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc
    12541 ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg aatacgaata
    12601 ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag
    12661 agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt
    12721 tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc
    12781 gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact
    12841 tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt
    12901 atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat
    12961 tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc
    13021 tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt
    13081 tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc
    13141 acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat
    13201 tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg
    13261 aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga
    13321 tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa
    13381 acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg
    13441 atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata
    13501 agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc
    13561 agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg
    13621 aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg
    13681 ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg
    13741 ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc
    13801 tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga
    13861 tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa
    13921 ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta
    13981 tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag
    14041 gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc
    14101 cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt
    14161 ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa
    14221 tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa
    14281 gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg
    14341 agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa
    14401 gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt
    14461 gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg
    14521 tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag
    14581 ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc
    14641 gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca
    14701 atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat
    14761 cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa
    14821 tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc
    14881 ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa
    14941 gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt
    15001 tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg
    15061 cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt
    15121 gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt
    15181 gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt
    15241 ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta
    15301 tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca
    15361 gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa
    15421 gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct
    15481 gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta ttctcgtaaa
    15541 taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg tttttaaata
    15601 aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt
    15661 aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca
    15721 gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa
    15781 aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc
    15841 gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg
    15901 atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg
    15961 aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat
    16021 gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat
    16081 gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc
    16141 tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc
    16201 atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta
    16261 ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc
    16321 attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa
    16381 ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc
    16441 aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata
    16501 tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag
    16561 tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt
    16621 cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt
    16681 agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc
    16741 tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca
    16801 taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt
    16861 ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta
    16921 ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt
    16981 ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg
    17041 gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct
    17101 aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta
    17161 tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa
    17221 aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag
    17281 tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc
    17341 tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag
    17401 aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca
    17461 aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt
    17521 gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga
    17581 agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat
    17641 gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg
    17701 ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt
    17761 tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc
    17821 actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg
    17881 tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc
    17941 tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa
    18001 aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat
    18061 gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg
    18121 atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac
    18181 gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat
    18241 cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt
    18301 atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga
    18361 ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac
    18421 cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat
    18481 ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt
    18541 atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca
    18601 tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac
    18661 tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac
    18721 ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa
    18781 acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga
    18841 cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga
    18901 tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc
    18961 acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga
    19021 cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc
    19081 gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat
    19141 gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga
    19201 tgaaatactt gcacatatcg atcgaatccg agataatacc tatgcttatc tgattgtaga
    19261 tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag
    19321 cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa
    19381 aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa
    19441 cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat
    19501 tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt
    19561 cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca
    19621 ataattcctt ttattcaaaa aacataccaa aaata
//
LOCUS       DAJSBC010000048        19475 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSBC010000048 DAJSBC010000000
VERSION     DAJSBC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.048"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(21..995)
                     /locus_tag="ONT65_001841"
     CDS             complement(21..995)
                     /locus_tag="ONT65_001841"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2329451.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ONT65_001842"
     CDS             complement(1075..1950)
                     /locus_tag="ONT65_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR2329452.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ONT65_001843"
     CDS             2080..3987
                     /locus_tag="ONT65_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR2329453.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ONT65_001844"
     CDS             complement(4528..7806)
                     /locus_tag="ONT65_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR2329454.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="ONT65_001845"
     CDS             complement(8077..8622)
                     /locus_tag="ONT65_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2329455.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="ONT65_001846"
     CDS             complement(8634..9326)
                     /locus_tag="ONT65_001846"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR2329456.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="ONT65_001847"
     CDS             complement(9345..9629)
                     /locus_tag="ONT65_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329457.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="ONT65_001848"
     CDS             complement(9642..10205)
                     /locus_tag="ONT65_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2329458.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="ONT65_001849"
     CDS             10433..11095
                     /locus_tag="ONT65_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR2329459.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="ONT65_001850"
     CDS             11219..12415
                     /locus_tag="ONT65_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR2329460.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="ONT65_001851"
     CDS             complement(12516..13892)
                     /locus_tag="ONT65_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2329461.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="ONT65_001852"
     CDS             complement(13929..14795)
                     /locus_tag="ONT65_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR2329462.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="ONT65_001853"
     CDS             complement(14884..15909)
                     /locus_tag="ONT65_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2329463.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="ONT65_001854"
     CDS             complement(15927..16601)
                     /locus_tag="ONT65_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2329464.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="ONT65_001855"
     CDS             complement(16785..17678)
                     /locus_tag="ONT65_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2329465.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="ONT65_001856"
     CDS             complement(17752..19449)
                     /locus_tag="ONT65_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2329466.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DAJSBC010000049        18599 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSBC010000049 DAJSBC010000000
VERSION     DAJSBC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.049"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(9..1644)
                     /locus_tag="ONT65_001857"
                     /pseudo
     CDS             complement(9..1644)
                     /locus_tag="ONT65_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2199..2762)
                     /locus_tag="ONT65_001858"
     CDS             complement(2199..2762)
                     /locus_tag="ONT65_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2329467.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2777..3820)
                     /locus_tag="ONT65_001859"
     CDS             complement(2777..3820)
                     /locus_tag="ONT65_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329468.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3953..4357)
                     /locus_tag="ONT65_001860"
     CDS             complement(3953..4357)
                     /locus_tag="ONT65_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2329469.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4590..5225)
                     /locus_tag="ONT65_001861"
     CDS             complement(4590..5225)
                     /locus_tag="ONT65_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2329470.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5491..6543)
                     /locus_tag="ONT65_001862"
     CDS             complement(5491..6543)
                     /locus_tag="ONT65_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2329471.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6556..7494)
                     /locus_tag="ONT65_001863"
     CDS             complement(6556..7494)
                     /locus_tag="ONT65_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2329472.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7945..8595)
                     /locus_tag="ONT65_001864"
     CDS             complement(7945..8595)
                     /locus_tag="ONT65_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329473.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8695..9204)
                     /locus_tag="ONT65_001865"
     CDS             complement(8695..9204)
                     /locus_tag="ONT65_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR2329474.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9405..10274)
                     /locus_tag="ONT65_001866"
     CDS             complement(9405..10274)
                     /locus_tag="ONT65_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2329475.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10294..11415)
                     /locus_tag="ONT65_001867"
     CDS             complement(10294..11415)
                     /locus_tag="ONT65_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2329476.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11417..11812)
                     /locus_tag="ONT65_001868"
     CDS             complement(11417..11812)
                     /locus_tag="ONT65_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2329477.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(11831..12637)
                     /locus_tag="ONT65_001869"
     CDS             complement(11831..12637)
                     /locus_tag="ONT65_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2329478.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12630..13418)
                     /locus_tag="ONT65_001870"
     CDS             complement(12630..13418)
                     /locus_tag="ONT65_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2329479.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13432..13908)
                     /locus_tag="ONT65_001871"
     CDS             complement(13432..13908)
                     /locus_tag="ONT65_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2329480.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(13980..16637)
                     /locus_tag="ONT65_001872"
     CDS             complement(13980..16637)
                     /locus_tag="ONT65_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2329481.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(16656..17060)
                     /locus_tag="ONT65_001873"
     CDS             complement(16656..17060)
                     /locus_tag="ONT65_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCR2329482.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(17292..17549)
                     /locus_tag="ONT65_001874"
     CDS             complement(17292..17549)
                     /locus_tag="ONT65_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR2329483.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(17674..18006)
                     /locus_tag="ONT65_001875"
     CDS             complement(17674..18006)
                     /locus_tag="ONT65_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329484.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         5883 a         3757 c         2748 g         6211 t
ORIGIN      
        1 aaatgtaatc agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa
       61 agaaagaggt aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg
      121 gaagaaactt caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc
      181 aatctctctt ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag
      241 cgttattttt tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg
      301 ataaggcata gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt
      361 gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta
      421 acgaataaga ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc
      481 atagtttgct tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt
      541 aactactttt ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga
      601 gccaaacagt acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt
      661 attttgtaat aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact
      721 aaggtaaatc actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa
      781 agaaataata ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat
      841 caaactatcc gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat
      901 cgattgaacc atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt
      961 cattcctact gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc
     1021 acttactaca aagccgccta agactagtaa gccaacggct attacactca taaaagtgga
     1081 aagaacatgt gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt
     1141 tttctcataa atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat
     1201 cgcttcagca atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata
     1261 taaacagcta aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa
     1321 catcagcatg atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa
     1381 attattactt atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata
     1441 attcactccg ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga
     1501 agataaccct ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg
     1561 aacatttcta aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa
     1621 tattattttt ttctttactt gcattactta attatatact cctttcatgg attgctttat
     1681 ctagtataac ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca
     1741 ctaaaattat aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat
     1801 tgcttaacta atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt
     1861 gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg
     1921 gatatgacta tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag
     1981 gcgctgcgta tcatgagtga atgatattag gaaaacttca taaaataaat cttttataca
     2041 tttccacttc tagatggtca ttcatgttag aagaatcaaa ctattttttt actgtcgtaa
     2101 taatattaaa acactagaac aaatcaatta aattccataa aatataaata atagacctag
     2161 catcatctgg tataaaatgg atgctaggtc cgttatattt actcaaaatt gattctacga
     2221 atagatcttc ttatcatttc tattttagta gtcatgcgtt gttgttcttc taaaggtggt
     2281 aatggtataa gtagtttaga taaatttgta ccatttacat ttggttgccc agttccgtaa
     2341 gaaatttttt ctaataactt ccaatataat ggcgagtcta agaagcagtc tacgtattct
     2401 gataataatt tctcaactaa cctaattcga atcaagtaag aagcaaaaac agattcctca
     2461 cttatttcct taaccaagaa actttttccc atagttcctc ctgtccttgc tatcaaaatg
     2521 tcattttctt ccaatcttaa atcaactagt ttagaattac taatatcaca gtaaggcaca
     2581 ctggaccaat ttacacgtcc ctcttgtata tctgtgattc tcaaaaactt aacattgcct
     2641 tgttttttcg ccgaatctgt atagccatat tgaatcttag tagaaatact tcctaaataa
     2701 acccatagtc gaacatcgct agatgatttt tcttgtcgtt ctttatgaac aacagtgatc
     2761 atcttgagtt gtccgactat tttttagttg cagcaatctt tttgggacgc ttatttgcat
     2821 gaagcaacgt tgctaagtct agtaataaaa cattggctaa cacaggattg gctttctcgc
     2881 gatacgctgc ggcgccactt tcaaacaata ctgctagacg atcttctaca acaacgattt
     2941 gttccaaata cggtggaaat ttgattcgtt ttaggatccg gctaggagag tcccgctcgg
     3001 acactaagag ttccgaggta taaggaccat aagaacgact ggctaagaga taatggccat
     3061 aaaaagccac accttgaatc ttatcagatg tttttctgct cattgtcacg gttggttgga
     3121 ttttgtcttt gcggcgaatt gctgttttca tttcagctat cgtcggcaat agatagaaag
     3181 tcaactgtcc ttcacctttt agtgtgaagt tgccagatac cagttgatca ttgttttgag
     3241 tcaaatacga tgcttgtgga atctctgaga gatcagttgt cagccaataa gcgattggct
     3301 gtcccgtctc ttcagatgta tctgctaaga tatcgatcaa acgtaaagcg gctactcgtc
     3361 cagtagcaga tcctgtgatt gtcaaccata aaagttcccg ttttgtatca tatactaagc
     3421 cccctacatg tggccgttta ggtaaaatca gcagtttgat cggctgtcct gtttctctat
     3481 ccagtacatg aataatagaa tgatgctgat ggtcatagca gtaggcagaa ataaacacaa
     3541 aatcttgaga aaaagccaac ccttgcggcg tcatccattg acaaacctca ttttcgccgg
     3601 tttccggcac caccattctt gtttctgtta atccaggaat gttatattgt tttgcttcag
     3661 ccttttccca agctgcttga acaataggat accgttttaa ctttttttca atatcctgat
     3721 gtatccgttg ctgcttccaa tgcttaaact ttaggacata gtaaacaaga aggccaatga
     3781 ccagcaacac taaaatcatt attgctaccc gctgtgtcat ttttttgcct ccttgttcta
     3841 ttcgatcaat cgtccatata ccgctattaa ttaccgtaac tacatgtttc ttttactagc
     3901 gtttctttat aggatctttc ataatctcat ttatagtaac attaagaatc ccttattcta
     3961 ctatagtcac ttccacatca atattgcctc ttgttgcttt agagtatggg caaaatgcgt
     4021 gggcttcttc tgcgattttc tctgcttggt ccttctcaag tcctggcaaa gaaacttcta
     4081 aaacgacccc gatttggtag cccccatctt ctaattgaaa taaagatacc cgtgctttga
     4141 cttcagaatc agactcaaga ttattttctt ccagcatgtg ttgaaccgcg ccgttgaagc
     4201 aagatgcgta tccagccgca aaaagttgtt ccggatttgt cccttcacta tggattcctg
     4261 ggcggtcgat cttcatgtgc atacttccgt ttggtgcctc aacttcgcca tcacggcctc
     4321 cacgattgat cattgctgtt tcgtacattt ttttcattga gatcatcctt tctttcgtcg
     4381 ttctatatta agtgtaacga gtatttttta atctgacaaa ggtaatgctt gcattcggcg
     4441 acatcagtta gacaacatcg atgatcagtg atagaagcag tttatctata caaaaaaaga
     4501 agggttctat gatatttggt gtccttactc gccttgagta acaacaccaa atatcataga
     4561 accacttaat cagcttcaca ctgctttttc tactttcgta gttgaaaaaa acttaaacaa
     4621 atcatcggca aatagttaaa gaaaaaactt aatcgttgaa agaagcccaa ttgcttaaag
     4681 aaggcaaact gactaatcga cggaatccgt gctaatccgt aaagacctgc aattagtaaa
     4741 ctaataactg ttaaaacaag ataaaaatgg ctaagcgtac cttgaccact ttgacgataa
     4801 agcagaacaa gtaacaatgg gaaaagtaga aagcctgcat agcctagccc agaacctata
     4861 ttatggatcc aagtcgatag attacaagag ctttcattcg tattgatact gaataagcca
     4921 gtaaaaatac aatcacctat cccatataaa ccaactgttg caacaacaag tattgcaagt
     4981 gtttttgatg ttccaacaaa taggtgatac aacgcaggca acgaaagaac aaaaaaaagt
     5041 ccagatacaa ctgaccagac caagaaagca cgtctgactg gactatctac gtcaccaaac
     5101 acactaatta cggtagtcat ttgattcagt ttgggataaa aaaggccaag gatatacggt
     5161 gtcgataaat cactcatcac acctaataat aaaaaataaa aaccatattt ttgcaaaaaa
     5221 atcattttct tcccctactt tttagtgatt attcgataca tccaacaatc aaattttttc
     5281 tcattttaac acatattttt attctagttg tcttttgaac aatcaataac ataaaggtga
     5341 gtttcttcta cattttaaaa aatcgaatgg cactgaagag ttcagttgtc agcatcaatc
     5401 tcctactcaa aaaggcaaaa aaacgtaagt cagatgttgt tgacatctca cttacgcatc
     5461 cattgattga aaatttctta gtaaaatcta ctagcctaaa aatttgaaga cttcttctcg
     5521 atcatacagt ttaaatactt tctcgcataa aaattgagta attagtgccg caaaaacagg
     5581 gatgactaac caacaaagaa caacaagaaa gccatttagc cctgcatcta aagaagccaa
     5641 cggaccaaca agtccgacca cgccgaaacc agcagaagca ggcgtacctg aaatagaaaa
     5701 tagagcgaca ggaatagctg taatcactgc actaactaaa caaggaataa gaatcacagg
     5761 ttttctaaat agattaggca tcatcatctt catagcgcct aaagcaatcg caattgtcac
     5821 acctgactta ttaaccttcc aagagttcac tactaaaaca aaagtggttg cagctacgcc
     5881 catcgcagcc gcaccagcag aaacaccatc tagttggatt gccattccga ttgctactgt
     5941 tgaaatagga gagataatca aaaaggcaaa ggaacaacca atcaagatag acattaacag
     6001 cggttgtaag gtagtgaagt tattaatcaa tgatccaatt gcagaagtaa ttgctgttac
     6061 aaaagggaat aaataaaaac caagtaaacc tgcacctaca cctacaacaa tcggtgttaa
     6121 cacaatcgac actgacccaa aactatttcc aatcagtaaa atcagcccga cagcaatcga
     6181 tgctgtcagc atcgtattaa taatatcgcc tgtaccagca ccaacgtaaa cacccgtttc
     6241 acccaaatca ggtgtgaatt tgactacacc agaaccaaca aaagaagctc cggctacaac
     6301 catcattctc atcggatcaa atccaaattg taaggcaatc aaaccaccaa tgatcaaagg
     6361 tgttgctagt tgaaaaataa ctgctaactg tgcgatcgtt accgcaaagg gatacgcact
     6421 aaaatatttt aaaatggcac ctaatactgc attaggaatc aatccaataa tgattcctgt
     6481 tgctgtacca gcgaggactt tattcagaaa aattttcggt gatatttttt gtgaagtttc
     6541 cattcaatct ctcctttatt ttacgttcaa actatcttct tttgcatgga caagctcagt
     6601 caagaaatca caataaggcg tcgcaacacc atatttcttc ccttttctag aaattgcgcc
     6661 attgatataa tcgatttctg ttaatcgatg gttcttaatc aagtcttgat acattgaagg
     6721 ataatgtaac ccaattgttt ccgggtcaaa acaagattca caatgtgcaa tgacttctgg
     6781 gacatctagt tcaatcttct ctactgctgc tactttggca aactcattga caatcgtcgc
     6841 caccatttta tgagcagtcg atgtttttcc tagctcagcc atattaacgt ctaagattgt
     6901 gcatagtccg ttcattgttc cgttcacaca tgctttgcga taaatagaat agtgaatatt
     6961 gtcagaaaaa tgagcattta atcctgattc agacaatttg tctgctaatt tttttgcagc
     7021 tgcttctttt ccatcaccta aattttgtaa ttctactgaa ccacttccaa ataatttgac
     7081 ctgaccagga ccttcaagac ctgccgtcca catggtattt ccaatataga tattttccat
     7141 tggtacaaat ttctcaataa tatcttcatg cccgatacca tttaatagac ataagacctc
     7201 tgtatctttt ttgattaatg attggatatc ctgcagcatt ttttccagct gcatcgcttt
     7261 ggtaaataga ataatcagat caacttgatc ttctttttct acttcggatt gaagaacgat
     7321 tggtagtttt gcttctacct ctttaccatt aaaatttgct tgtaatccat gttctttgat
     7381 ttgttggaca tgttctgccc agccatcgat taataatact tcgttcccac tttggtgaag
     7441 catcaaccca aagcgacttc ccattgcgcc agcgcctgca attgctattt tcattgattc
     7501 ttcctccttt ttctttctat tatactcatt tgagaatctt ttcacaacct cgttttgtta
     7561 ttcttctctt atttatcatg tttatatcca ccattcaagt tattttatca gcaaatcaat
     7621 aaaaactgga atagaatccc aatttcatcg taagagaaac tagactagca ataagaaaat
     7681 agaaacattc atagaaaaaa tctcactcct ctgattataa aagagggggc attaacctcg
     7741 tgtatactaa aaccttccat tcattcgatg gatgattttc tttttgtgaa aaaatattca
     7801 ttgtagtacg atgttgcgca acacagacgg atggtattgc ttattattta ttaaaatcga
     7861 acaatacact tatttaaaaa cttctttccc agattttctt ccatttttac agttataact
     7921 agtattatca tgaaaaatag ccttttattg tctatgggaa taataattta aaattggtac
     7981 ttttttatta attagttcat cgcccaataa tctctccagt ttcttagcat ctcctgactc
     8041 aaccggaatt atccaagccc agtctaaata attttttaaa atctcatcat attttgcaaa
     8101 tatttcattg ccatattccg tccttaaaat tatattttca gtgccagaca agtgatttat
     8161 attgagttga taaaaaagat ctccatctaa aaaaatatca ttagaagaag atttccccac
     8221 agtaatgacc attccatcag ctctatgtgt tacatacaaa taattgtatg acctcatttc
     8281 acctaatgaa tcaattattt cttgcccttt agctctaata tattgatctc tattacctat
     8341 ctcactctta tttaacaaaa gttttttcat gtcaaattta atcggctgtt ttatttttgt
     8401 aaattcgctt ctactactaa tccaatccga gattaatttt tcaacatgtc ccagagtaaa
     8461 ctctgattta ttcgctaaat actttttttg ttgtattaaa gacatctttc cccgaagctc
     8521 aaaattgagc ctacatcgat tctctatctc ttcaaaaagc atcttatctt catcattctt
     8581 atattcatca ttcataaaac caatccagct cctcgattaa gataatattt taatctcata
     8641 caaaaactag ctctcatcaa aacaaccatt gttttgattt ctcatgaaat ttctttatac
     8701 ttttctccga ttataaattc ctaacccctt gagaaaatta cgcatatatt tatctccgca
     8761 aggcttaaaa tttcgttggc cttcttttct caaaaatgcc ccgatttcgc cttttgatgg
     8821 atagatttct gcctcagcaa aaacgtctaa aatatcatca gtggtcatag acaaggcgat
     8881 ttttaatttt tttagcaaca cattgttgat atatttgatt tccttgcttt tttgcggcgt
     8941 gtctactatc ggcttatctt tttgcggacc acgcgccaag agaatcaggc catttaggaa
     9001 tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca ttattttctg cattatcttt
     9061 gttcttatcc tgtactttgg tcagtaccgc ctgagcttct tctttagtca gtggtacacc
     9121 gcctagctca aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat aacgcaatcg
     9181 caataaccta tcattgttat tcatttgttc ctccgaagta tttagtcttt actttcttac
     9241 gtactttttg tcttgctaag atttttagga tcaatgataa aaaccgcatc acttatgata
     9301 aggcgctggg tatcatgagt gaatgatatt gctgaataca aatagtccga ccacattaag
     9361 ttgatcggac ttcataagca ttttattgac taatactttt ttgcttaaga attgattcta
     9421 acatcttttg ctaaagttaa gcatcctata gttcctgcat tatctgctaa cttaggtgtg
     9481 acaatgtatt cttcgatatt tggcgtatcg acatagccgt taattagttc taaaaaacgt
     9541 ctttgtactt tctttttcag atgttcttgt tgcatgacac caccaccaaa aattataata
     9601 tctggtgaaa gcatcagggt cgtattacag gcacattgtg caatataatc cgcctcgatt
     9661 tcccagaacg gatcttctat agaaagatct tgtcccttgg ttcccgtacg catctcgata
     9721 gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt accagagaaa
     9781 gtatctcctg aatgtctctt aactaacgta tgccccattt ctggatggct aaacccttca
     9841 acaaaaatcc catcttgtat agctcctcca ccaattccag ttccaacagt gtaataaaca
     9901 acactggata aaccttttcc atgacctgaa acatactccc cgtaacaagc agcattcaca
     9961 tcggttgtcc aggcaattgg aatttcgaaa tgcttattca acgttccaac aaaatcaaaa
    10021 tttttccagg cagtttttgg agtagaggtg atatgtccat aggtctttga ttcacgatgg
    10081 atatcaatcg gtccaaatga ccctactcca atgctttcta attgttcttt atagtgatta
    10141 aagaaatcaa ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa actcactctt
    10201 tctaaaacct ttaaatcatc attgccaatg gcacaaacaa atttagtccc acctgcttct
    10261 attgagccat acatttctta ctccaccgtc cttttatcta ggaattcgtt cttccctgtc
    10321 taatagtttt tcgaaaaact catgtggttc cttttggtac caataggcaa cagaagaaat
    10381 atcgtcttga cgctcataaa gcttgatgtc atcatttcct agggtttgga aagtaacacg
    10441 taaattttct ttgaaactaa tggggtcggg taaatgccag cggtacaacc catgcatcgg
    10501 taaagcatca tcgccaaaac catgaactgg atttggatta ggttttccag cactaaaata
    10561 atcccgggtg tgatctcttt tagattgaaa aggatagccc ataaatagcg tattaaaatt
    10621 ttgagcatat gccctttctc caaagttctt tttatggaaa gcccaagcgc cgccaaaata
    10681 atcttctgct cctgtagatg ttattgtagg gaaatcttca tctccatcta tataaaactt
    10741 aaattctcct tccccccacc agtatctttc caatgcgcac aaagcaaagt aggttcctac
    10801 ataataccca gtgcctttga ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat
    10861 gggttcacgg ttccatttag catgaaaata catcgcatca tccggtactt cagtcaatgc
    10921 ataattaaat gtataaaaaa tgtgcttaat aaactcagga tgttcattcg ttaaagtaat
    10981 cttagctttt tttcgaaatg gcatctcaaa atagcaattc atccctgatg ttggattcac
    11041 tacgattggt aaggaattca cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc
    11101 taatggactt tctacagaag gagtggattc atcatcccaa taaatacgca aaattaaatc
    11161 acgtaagacg aaactcccag cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa
    11221 aattccaggg ccttccattt ctgctaaagt aattgtctct ccagcattta tcggaacaga
    11281 aggtgaccct tttctcccaa ttcctagatg actcgctgtc attgccgctt tccctttttc
    11341 accattttta ttttctggtg aaatagttct actttcctct cccttgtgga gccatatatt
    11401 atttaaacaa ttcattttat tcgtcctcta tcctttcttc acttttttga ttattcatat
    11461 agatgatttg ttgttgtgcc tccgcaataa ttgctccaat atctaaatta tcatttccta
    11521 actgcaaagc gattgacatt gccaagggaa gattcattcc tgcaattacg tgcactctgt
    11581 cagaaatata ggtaaaaaac ttttgattta cacttcctcc agccatgtca gttaaaacaa
    11641 ttatttcgtc tgtagtatcc actaactctt taatttgttc atctatatca tcaatttctt
    11701 cgacatatgc acataagcta ctaatttgat tatgcatacc tgttaaaaac tctagtgtct
    11761 ctttaaaacc ctcagctagt attgaatggc tagcaatgat caatcttctc ataattcaaa
    11821 tccttttcct ttacgctaat attccaaatg cagccataac catcgcaaaa acaataacga
    11881 gtagaatcaa aatggtcata ctgatttttt tcttactgag tagataatat gctgcagcag
    11941 taataattac tggaattaat gttggtaaaa ttttgtccaa cattggttgg attttcatac
    12001 taacttcacc aaaagtgaat ttaagtggcg ttgaaacact gataactgaa gcaattaatg
    12061 atccaactac tgttagcccc aatactgaag cagcttcagt taaataatta atcttttcac
    12121 cgtattcagt aacaatcttc attccctgct tataaccaaa ttcaaagcct ctcattcgga
    12181 aaaagaagat taccatgatt aaaatgaacc ataaaaagat tcctacagga ttattttcta
    12241 aagccatata agccgcaatt gatcccatga tggttgggat catcgtcatc aaaagagagt
    12301 ccccaatacc agcaattgga cccatcgtac caactttaaa gtcattaatc gcatcttttg
    12361 cttctaatcc ttgcttttcc tccattgcaa cacaagcacc taaaataatg gctgccagcc
    12421 aaggctgtgt attgtaatac tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat
    12481 ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt cacagatgca ccttgctgtg
    12541 tttgataatt aaaggtacaa actgacatca accaacgata gttcgctttc ctaagatcct
    12601 ttttcgttac ttgatatttt tcgctcattt tattcatcct ccccaataaa cccaacatca
    12661 gaagctcccg cagaagcagt gatttgtgca tctttttcag ctctatgata cacattaaat
    12721 gctaaagcga atcctatgat cgccgctcca ataataggta ccttcaaaaa agcggctaag
    12781 aaaaatccaa caagtaaata actaatgtat cgacctgtag gcataaagcg taacaacatc
    12841 gcaataccaa cagcaggcaa catttttccg gctaatgtta gcccatccat aaaccaatta
    12901 ggtaaaacat ccaaaacgta attaacagca gactgtccta aagtaacggc aataaatact
    12961 ggaattgctg cagataaacc aaagaaaatt ggccccaacc acaaaacact attcattttc
    13021 ttaaattctc gtttattcgc atattcctgt gatttgcgaa caacaaaacc atttaagatt
    13081 ttgacaatta catctagttg cacccctaac atacctacag gtagtcctat tgctaatccg
    13141 gcttctactc caagctggct accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc
    13201 ggtacagatg agccaccaaa accggcaact cctaatgaca tcaattgaac tgtaccgcca
    13261 actatcaaac ctgtttttat atcacccatc aaaattcctg tgaataatcc ccaaaaaact
    13321 gcgtaattta caaagatcat cggaatccca taataatcaa catgtttaat aaatgctaat
    13381 atagtcagca taataatttg aaaaatagta aaatgcatta tccttactcc tttatcctaa
    13441 ttttgattga atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa tttctacacc
    13501 agaatccaac aattttttat acgtgctgat attttcatct gatgcatagg ctctagaaga
    13561 tagctgtaat gcattatctt taacaacagt accgccaaca attagtcggt caattctaat
    13621 accgttatct actaaatgaa ggattgtttc cggctctttc acgatgacaa atactttttc
    13681 ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt tcttcagtga taattgatac
    13741 agccattcca gcaggtctag ctaaacgcat acttgatttt tggatgtcgt tatttgcgac
    13801 agaatcatcg atcaccatca ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat
    13861 aatcccatgc aataaacggt tatcaactct cgacataaca atactcataa atacttcctc
    13921 ctttatcttt cattttctta caagcaatta ccatgccaaa acaaaaagaa cttgataaat
    13981 caagctcttt ttacataatc ataaacgtat ttaacctcat gaatcgggat gtcgacacta
    14041 taataatgtt caacgacact gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc
    14101 tcactcgttt cattgaaata aatttcatct tccaaagtat tttgttttac taatcgttca
    14161 attaagcaac taatgtgaac atataatccg aaacacgtat tattatcaaa tgaaattcct
    14221 agatcatttt gtaagatgta tatagcatct gaaacccgtt ctaataattt agtcggattt
    14281 aatatggtca attgctccat taaattcgtt agactaaaat tcttcagaat attattgttc
    14341 aaaactttca gttcattatc attaaagtgt ctattcaatt gattggagaa tttacttata
    14401 ttcattccta aaatcatatc ttcaattgaa aagaattcta catcttctag atgtggattt
    14461 agtgttccaa caataaaaag aatattgttc tctttacgaa gctccgataa atagttttca
    14521 ttcactaaag agtaatagtc acacgtttct atgacaagat cagtatccct tggaaagcta
    14581 tcttcaagta tcttttttaa tttaaacgaa gttcctaatc cgcttgcaca agaacagaca
    14641 atcatatcct tcttaatatt tgatgattca taaatataat ccacattttg attttctttt
    14701 acttgttcaa gaatctgttt aagcggtaaa tctaataata tctgatttgc aacatcaata
    14761 gccattcttg ttgaaacatt gtttagtagt ccgaattttt tttgagcgtc tagttccaaa
    14821 tttagataaa ttttctccag tgaacccata tcaacaagta aaataatttc ttcatatccg
    14881 gtacactctt gtaagtatct attaattttg tctgcaatct cactacttga agtcattagt
    14941 ggcatatcaa tggcgtcgta tatatatctc tcaacgatcc tattagcagt acttgctata
    15001 caggttgccg ttagatctcc atgagccaaa atcagtgcta aacgtttttg ctgaaattct
    15061 ttactacaca ttaacttaaa taaaataacc aaaacatttt tttgaatttt tgttaaataa
    15121 atatccccta tttgaataaa acgattgact aattgaactt ctcgactaca ccaactatta
    15181 atatcattga tgaaggaata taatttatcg tccgatgtta attggatttg ataaatccaa
    15241 gaattcaaaa agatagacgt tatttgaaga taataatagt caaattctat ttgaaaatct
    15301 ctctctattt ccttaacata ctgtctaaat cgaatctctt gataggtggg atatgttaaa
    15361 gctgaattat tttggatatc aacattcttt tgtctaaaat atctatcata tttctcaaaa
    15421 agctgctgct tatcagtgtc tttcttccac gtttgataaa gatactctat aagttcgttg
    15481 atattaaaaa cttcgtctac tttttccaaa tcatccattt gaatatattt taattcattc
    15541 tgtcttttca taaccttcat gccgttgaaa ttcacccatt gtggtaaatg atacatctga
    15601 atataaagtt cttccaaatc atctaagaaa taaaaggcat tggcacaggt caattgaatg
    15661 atatttttca aattgccaac attttcacta aagcgatagt ttaataaaaa atctaataat
    15721 tgttgagata tcagtatttt ctttgctagt ctttcttctt ctgatttaaa aaatatcttg
    15781 attaactgtt ttctttcata aagagaacga ctgtctaatg ttggaagtgt tagctttaca
    15841 ggtattcttc tcaacaaagt tgataatagt ttatcctcag gtggttctga tgttgcaaat
    15901 ataatccagc aacttgatgt gtaccagttt tcattatcac ccatccgatg aaatttcctc
    15961 gtatccataa actgaaaaag tttctcctga cattcattgc tcaatgcatg aatttcatct
    16021 aaaaataaga ctccattatt agctaattct gctaaaccct ttcttccatc ttcggctcct
    16081 gtatatgcac cttttacatg cccaaaaaaa ttggacaaga aaagttctgg attattggca
    16141 tattcactac aattaactga aatgaaagat gccttctctt ctatataatt attggccaac
    16201 aaatccttat acacataatt tgctaaatag ctttttccag ttccagtctc gccatgaagt
    16261 aaaatgggta agccatttgg cggatagtag attgctgctt taatttgagc aattacattt
    16321 cgcagacttc cttgacttcc gataaccctt tctattccaa ccctaaaaga gctatcatac
    16381 catttttccg cttcctctga agagctaaaa atttgttgat ttggaacttt ctccggtaaa
    16441 gaaaagaaga ttactggacg agtattgatt tttccaaatt ctccatcttt tacgaattca
    16501 ttcaaatatt gagagacaat attacgacta acatttaatt tttcagatat tttctgtgct
    16561 gtattgttcc cccatttatt tttaatctca gagttctcta tgacataatt gacaatgcgt
    16621 tcttttaatt cttccatcac agcacctcct cgacactatt cgaattttgc cgacagtatt
    16681 tttccataaa tatgacttgc taaaaaggct ttgtttagat cattcaattg gaacgttgct
    16741 tgttttgctt gatccgctaa gacgttgaaa agatactctg gcgaaatcgc ataccccaaa
    16801 atatcaacga ttgttgcaat cagatcttct tttgattctt catctgaaag taattcacga
    16861 tacaacatcg tttgtttgct tactgtgtca tattcctcta aggattcatc tgctaaaaga
    16921 accacttgtt ccaacaaacg gtcggataaa tatttataga aaatcagccc caataaataa
    16981 tttttatatt ctgacgcatc cattttacta cggagattat ctgccgcact aaataacttc
    17041 tgatttaatt ctgcacccaa aaagatacac tcctatctct tatttaaagt tgctatatac
    17101 cctcttattt tagcatactc agttttagaa aaagcaaaaa aataaagaaa cttattcttg
    17161 ataacaagct acgtgacgta tatccctaac acaaaaatag ccatatcctt tggtttggat
    17221 acggctattt gatattactc attcaattat caaatcacag taggtgtgaa tctcatcggg
    17281 atcggcaata tctacttctg attaggatca aaggtgattt tctcaataaa ggtgtgatac
    17341 aaagctttaa cttgcttctt ctcactctgg ataagaaagc aatccaaact agtcaatatc
    17401 tgttggacat ctttgacttg aatcggttcc ccttgttgtt gaaagatccc taagatttca
    17461 ttctctcgaa tttgtaactg tcttcgtttt tcagtcaatt catttaaacg atcttttaac
    17521 atcggaaaca attcgggact atcttccaaa gcagcttcct gtttttgctc taatagttag
    17581 attttgcgta ccattttctt cttttatttt ccttcgattg ctgattttaa tatagtctaa
    17641 aggaaattta tatttattgt attctttata acttcaagag aacttcgttt gagtattact
    17701 gtattttttt aattccataa gaaaattagt tattacctgc cacctaatca gcaaatctga
    17761 acctgagtca atataattaa tattattttc catctccaac attgtaagta ctgttggtgt
    17821 taatataggg actttgtaca attgtatttt tttgatttta tcatctgcac aaaggagatt
    17881 ttttaaatct aatccttctt ttatttcaaa agttgaattt tttaaatcta taagagtaaa
    17941 accaattcct tcatactgaa tttctgaaaa tcgaattttt ctcccagttg atagatcaag
    18001 gggcagaaaa ccttttatta cattgtgagt gtcaccatca tttacaatgt cattaggaat
    18061 tttgatttta gaattctgtg gagcacctat tggaagataa taattcatta taattttgta
    18121 gtcttcatga ggaaataaag tacttttagg attgctaaga tattctcgta ccgaatctaa
    18181 gaatggctta tccattgaat cttcatcccc atttaaacta aaaaacattc ctacaattgc
    18241 ttttgcaact ctaagtgaat tcattttctt tattgaatgt gttaaatcag acttaccatt
    18301 ctttaaactt gtattgttat aaaggaaatt tttattgtct tctgcaaatc taatatattc
    18361 ctcaacatac tcactaccaa aaaaactatt acatttacta cataacgtat tggaatggca
    18421 acagtttttt tgacaaattt tataaggtgc agaacttctt tccgtatgct attccgattg
    18481 tccttgacaa tgagcctcct cggatgtgac gatcttcggc aggagctaac agttaaagtt
    18541 agactgcctc gctagcgtta gcacatccga gctcattatc aaggattcgc tgcgccaat
//
LOCUS       DAJSBC010000050        17813 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSBC010000050 DAJSBC010000000
VERSION     DAJSBC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.050"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..348)
                     /locus_tag="ONT65_001876"
     CDS             complement(22..348)
                     /locus_tag="ONT65_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329485.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(963..2165)
                     /locus_tag="ONT65_001877"
     CDS             complement(963..2165)
                     /locus_tag="ONT65_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329486.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            2282..2797
                     /locus_tag="ONT65_001878"
     CDS             2282..2797
                     /locus_tag="ONT65_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2329487.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2996..3442)
                     /locus_tag="ONT65_001879"
     CDS             complement(2996..3442)
                     /locus_tag="ONT65_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2329488.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3489..4967)
                     /gene="msr(C)"
                     /locus_tag="ONT65_001880"
     CDS             complement(3489..4967)
                     /gene="msr(C)"
                     /locus_tag="ONT65_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2329489.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(5321..5950)
                     /locus_tag="ONT65_001881"
     CDS             complement(5321..5950)
                     /locus_tag="ONT65_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2329490.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5934..6398)
                     /locus_tag="ONT65_001882"
     CDS             complement(5934..6398)
                     /locus_tag="ONT65_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2329491.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(6885..8153)
                     /locus_tag="ONT65_001883"
     CDS             complement(6885..8153)
                     /locus_tag="ONT65_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2329492.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(8384..9121)
                     /locus_tag="ONT65_001884"
     CDS             complement(8384..9121)
                     /locus_tag="ONT65_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2329493.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(9188..9757)
                     /locus_tag="ONT65_001885"
     CDS             complement(9188..9757)
                     /locus_tag="ONT65_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR2329494.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9885..10757
                     /locus_tag="ONT65_001886"
     CDS             9885..10757
                     /locus_tag="ONT65_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2329495.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            11099..11971
                     /locus_tag="ONT65_001887"
     CDS             11099..11971
                     /locus_tag="ONT65_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2329496.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11968..13926
                     /locus_tag="ONT65_001888"
     CDS             11968..13926
                     /locus_tag="ONT65_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2329497.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(14258..15109)
                     /locus_tag="ONT65_001889"
     CDS             complement(14258..15109)
                     /locus_tag="ONT65_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2329498.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            15232..15699
                     /locus_tag="ONT65_001890"
     CDS             15232..15699
                     /locus_tag="ONT65_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR2329499.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15962..16444)
                     /locus_tag="ONT65_001891"
     CDS             complement(15962..16444)
                     /locus_tag="ONT65_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR2329500.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(16544..17407)
                     /locus_tag="ONT65_001892"
     CDS             complement(16544..17407)
                     /locus_tag="ONT65_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2329501.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         5315 a         3700 c         3146 g         5652 t
ORIGIN      
        1 ttccatataa acggcgttcg cctaccagct gcctggtaaa tctgtttcct cttcgttaag
       61 aagcgaaaat ccgtcaaatg tttcgccatc ataataacga agcagctcat ccatggacaa
      121 gttcatcgtt ttttcttttt cttcttccgc tgattcagcc agtagctgtt cccgatacac
      181 ctgatacata ccagctattt tttgctgcat cgcttttcta gtttcacctt ccgcattgat
      241 tattggcaat tctttgatat agccacgata aatcaaccga tcatctgaaa attcaatggg
      301 taattcaacg agtgtcaacc cgacttcctg aaaatattct tccagcatct attatccccc
      361 cattcttttt cttaaatgaa ttttagctta cctactgaaa attttccagt ttcattattt
      421 tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc cctttttccg aataaatggt
      481 gttcttctgc caactcctca atcataagtc acaatattca acaaacatga gaaactgttt
      541 tttgaaattg ttccttgtga ttcagagtta aacgacagtc tcacaacctc ttggtaaacg
      601 gcgttcaatc agctgaccct cggtattagc tcaaaaaacg gtaaaatatg agaagctatt
      661 tttccgtttt gtttctctaa tacttaggat aaaacgctga ttttacaacc tctgttaaac
      721 ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
      781 ttttccgttt gtttctctaa tactcaggac aaaacgctga ttttacaacc tcctgataaa
      841 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagaagc
      901 tatttttgtt tggctgttct tatgacttga tgcaggacag ctttttcaca acctctgatt
      961 tcttattctt cttcatttgt agcaaaataa ttacggtgat aaatgataca acatacactg
     1021 ataagaacaa tggcacctgc aaaataaaac gcatagcttt caccaatatg ttcaccaatc
     1081 acacctgcaa taaatggtgc taaagctcca ccgaaaaagc ggacaaaact atagctggac
     1141 gaagcaacgt ttcgttgcag acctggtatt tccatagcaa tcgtcgtcaa cagtgtattg
     1201 ataattcctt ggaaaaatcc tgctaacaca atgctgatcg gtacaaggac tggcatagaa
     1261 gttccagctc ctaataacag caaacaaata atgaataaga caaatgctgc taaaattgtc
     1321 cgataagtcg taaacttccg ctcaagagcc ggtgcaacga aaatcgaagc aatagccaga
     1381 agcacacccc agccaaaaaa tacaaaaccg acttctaatt cagaatagcc aatcaataaa
     1441 aatggcgcat acgcaagtaa agtaaagaat ccgaaattat ataacaatgc gattagtcca
     1501 actgaacgaa gtttatgatt gcttaacgct ttgacacctt cccaaaggcc tacttttctc
     1561 tttggtttct cagtttcagg taaaagtacc gctagggcta taaaaccgca aaacatcaaa
     1621 aatgctaccc caaaaaatgg ataacgccat gaaccactgc ccaagacccc accgactaga
     1681 gggccaacag ccattccgca gccaagtgct gcttcataca tcatgattgc tttttctgtg
     1741 ttaccaacta aaatactgac gatcgttgct aaagcagtag agacaaataa agcattcccg
     1801 acaccccagc cggcacgtaa cccaatcagc attccgatat tcccagataa tccgccaaga
     1861 ccagcgaata caacgatgat aaacaatcct gtcatcaatg tttttttcaa accgatccga
     1921 ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta actagtaaat
     1981 aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt taagattggg
     2041 tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac tagcgctgaa
     2101 cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt ctccaatatt
     2161 tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtca atatgcacat
     2221 gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag gtaggatttt
     2281 tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac gttctgggta
     2341 tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga tctatacaac
     2401 gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg agcactctca
     2461 aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat ggatcaaaga
     2521 ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg ctttttcgac
     2581 gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc tgaacaaaat
     2641 catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac aagaatttgg
     2701 acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc aatggtgtca
     2761 gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa cctttctaga
     2821 aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg aaaagaaaaa
     2881 gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta gaactgcatt
     2941 caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg ttccatcatt
     3001 cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg atcaactgca
     3061 acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag aataattgat
     3121 caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc attcctaaaa
     3181 ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt actggtagtt
     3241 taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg aaagccacca
     3301 cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga tgcagccata
     3361 aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta aaaattattc
     3421 cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg aaaatcatcg
     3481 tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt tatttcataa
     3541 acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa aatcgttccc
     3601 ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat aaaattagtc
     3661 ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat cgtcagacgg
     3721 gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg agcaaaccct
     3781 aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct catgacaaaa
     3841 cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac ttgaaagctt
     3901 actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa taaggttgtc
     3961 ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa ctgaaaatca
     4021 ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc catgatggga
     4081 tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt catctgctgc
     4141 ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc attcttttgg
     4201 atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccag tctgatttgt
     4261 tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt cttttcttgg
     4321 atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg tagctgagtc
     4381 tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa ctctcggatt
     4441 tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata gcgatcatga
     4501 ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg atcaatactt
     4561 tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata agaagaaaga
     4621 agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc tgaagtttct
     4681 tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc ttcagcgaga
     4741 taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc atgatcggga
     4801 aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt ttctcctatg
     4861 atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc tagctggttg
     4921 ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg ttttacactc
     4981 ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat ctgtttgcaa
     5041 aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa atcaccaaat
     5101 ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact ccatccatga
     5161 aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag ttcgaaacgt
     5221 aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc acacacgatt
     5281 caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac atagtgatct
     5341 tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct attgataaaa
     5401 taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac tgatctgctt
     5461 cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac atgaattgca
     5521 tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt gaaaaactct
     5581 tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc atcttccctg
     5641 attcgtaagc ttggttgtag atttcttcgc catattcgta acattgcata tcgccaaaaa
     5701 tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc aaaggacgca
     5761 agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggtttc atattgacag
     5821 aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt gggattttgt
     5881 atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa aaatcatttt
     5941 tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag ttgggtattc
     6001 aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc cggggatagc
     6061 acggaacctg tatctttatg gtccatttca caatgcttga ttttcttctg ggctttttct
     6121 gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga aacatagcca
     6181 tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg caacttttct
     6241 tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat ctcgtatcta
     6301 gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc ggctaatttg
     6361 atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt tctaataatt
     6421 gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt caaagaatca
     6481 gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa ttttttgtag
     6541 aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat tgattcttat
     6601 caaactatag aagatagctc ttctgcagta gataatttta caccgccaaa aatttccgaa
     6661 atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc ttaaacagta
     6721 ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt gttcaaaaac
     6781 ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt tttgtttggc
     6841 tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata accataaaat
     6901 cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt agcactcaag
     6961 gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat tggcttggaa
     7021 ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa agagttgatc
     7081 tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa acggtagaca
     7141 ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc tacagttggc
     7201 agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg acggttatac
     7261 agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag attataggtt
     7321 tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg acctgttagc
     7381 caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga atagtagtaa
     7441 acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa tgattctttg
     7501 tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt cacatattcc
     7561 gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa tagattgtaa
     7621 ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc ttgagcaatc
     7681 atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga ggcaatacta
     7741 tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata gccagtatag
     7801 ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc atctgtcaat
     7861 tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga tgtactgtct
     7921 tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat tgtattttct
     7981 gagacagtag ctgactgcaa agtactttct gtatcgtttt caattcctgt ttctgaagaa
     8041 ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt tgtgttggca
     8101 actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt catctttttt
     8161 ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt aaaagcaagt
     8221 caatgtccat atttaaataa catttgtttc tttttaataa atttgctttt tctgcataaa
     8281 aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt cgtttgaata
     8341 actatttaac aagtaattct ctctggatat tatccgttat ggattatact tcctgtgtcg
     8401 tcggacggat aatcatgttg ctgacagaca tccggtctgg cgtttctatc gcaaatgcta
     8461 cagcagaagc tatatcttca gatgttaatc cccattcttt ttgcgcttca tgaagttcaa
     8521 gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct cctggagaga
     8581 tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc atgattgcac
     8641 ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca tgtcccgcca
     8701 cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga agtacggcgg
     8761 aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat tcatcacggt
     8821 gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat acatcgattc
     8881 gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa tctcttacat
     8941 ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct tttaggcgat
     9001 cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc ttcgttgttg
     9061 cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat aaatcattca
     9121 ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc ttatttcatc
     9181 ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt cctgcaacga
     9241 tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg gcattctcgc
     9301 caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt ttcttgatgt
     9361 ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg ttcaccgtga
     9421 tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca cctaagtcaa
     9481 cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt ttgccgaaat
     9541 ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta atgatatttt
     9601 ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct gctaaaggct
     9661 cttcgttttc cgcttggatc tgatggatca cttcaaagag cggatccgtt actcgaatct
     9721 ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct tttatcctta
     9781 ctttataacg gacggcttat gatgtctaat acttataaaa catacttaac tattcctttt
     9841 aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat attcgtatac
     9901 tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca aaatctctgc
     9961 atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa cttggtgtga
    10021 cacttttcac tcgaggctcc cgagagatac agttgacgga ttctggtcga tatctttatt
    10081 caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta acaaatgacg
    10141 caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc tctttcattt
    10201 ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat ttatatagcg
    10261 ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt ggactcgtaa
    10321 tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa gatcgttggg
    10381 gacttttgat acatcggaat catcctttag caaagcaaac agtcatccat ccgcaggaac
    10441 tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa ctagctgaat
    10501 ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta ttgtacaatg
    10561 cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg atcatcaata
    10621 cttatgggac ggaaaaagta ttcatcccac tcgatccgcc attacaagct tcactgaatc
    10681 tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta gatgtcctaa
    10741 cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta acaacgaata
    10801 agttaagcaa aagatccaat gattaaataa cataagtttc ttaatttgtt ccttcctttt
    10861 atttctgata attccagtaa attactataa ttataaaaaa agaattgaca aaacataact
    10921 gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga tcatactagg
    10981 catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct atttttgttt
    11041 ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg gtgcgcgcat
    11101 gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg gacaggaaaa
    11161 agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga tttttgatcg
    11221 tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc aaaatttact
    11281 taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc atgcggaaac
    11341 tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc acattgcttt
    11401 ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa gagaaatcga
    11461 agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc tgacaaaaga
    11521 gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata ttcacagcgg
    11581 acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg tatctgacat
    11641 catccattta gtggaatcag aattggaaac agatatccat tcagaaccat tttcactgaa
    11701 ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg cgcaacaata
    11761 tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag aaagctataa
    11821 gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga tcacgaaaga
    11881 agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg tacaaacaaa
    11941 tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat gggacgttcc
    12001 cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat cggaaactgg
    12061 tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg taacgcagtt
    12121 ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc agtggacaaa
    12181 tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa tgttttaaaa
    12241 actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc atcttttgct
    12301 cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc tctttataat
    12361 cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa gcgtctagtg
    12421 ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct cgtatggcct
    12481 ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg ctggggtggt
    12541 gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca tcaagcaatg
    12601 aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt tttggcttct
    12661 gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat catgatgttc
    12721 gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa caaagcaaaa
    12781 acagcttcct tgatgttagc agcagctttt gcttcattct ttactggtgt aacagaacca
    12841 cttgaattct catttatgtt cgttgcttgg ccattatatg tcttgcatgc aattttcact
    12901 gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc ctttagtgcc
    12961 ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc attgatgttg
    13021 atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt tgctatcaaa
    13081 aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga cgatactgca
    13141 acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc tgcattaggc
    13201 ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt gcaagtcaaa
    13261 gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg tatgaagatc
    13321 atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt tgcagatgag
    13381 atggcaaaac tacatggtgg tgctgcaaca cgacctgcac aaacaaacac cgtggttaaa
    13441 acagaaacat ttgccggtgg aacgatcgaa caagatattt acgctatcgc aaatgggaaa
    13501 ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat gggtgacggc
    13561 tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac gatcacaaac
    13621 attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga agtcttgctt
    13681 catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata tgtaaaagaa
    13741 ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc tattcaatct
    13801 gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt ccaaagctta
    13861 gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt ttccaacaaa
    13921 gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag ctgagacaaa
    13981 aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa ctccgcggta
    14041 ataagtccag ccaaacgtaa catatgaaga gctatttttt atttgactct tcttattact
    14101 tgatgcagaa cagctttttt acaacctctg ataaacggtg ttcaaaaacc agtttatcgg
    14161 tatgaagtca aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttcatac
    14221 tcaaaactaa tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt
    14281 ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg
    14341 aaatagctaa ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt
    14401 cttgagggaa tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa
    14461 tttctaactt tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta
    14521 gattgccgga taattctaag atttctggag agtcactttc tactagtact tttgctgcag
    14581 cttctgcata ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga
    14641 attgaccagt ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat
    14701 tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat
    14761 ctggcgctaa tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag
    14821 ataccccagc tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg
    14881 gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta
    14941 aatcatcata atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt
    15001 cttcactacg tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca
    15061 gcgcataacc accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc
    15121 cctattcttt ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat
    15181 aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat
    15241 tctctgcaat ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc
    15301 cgatcacttt ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt
    15361 aaaatcatga gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag
    15421 ccttcccttg ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa
    15481 ggtaatacac agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg
    15541 aatatccaag aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa
    15601 atggctaaga tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa
    15661 agaccagaaa acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat
    15721 ttttctgatc ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc
    15781 atgccccttt ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa
    15841 acggtgttca agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct
    15901 atttcccatt tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt
    15961 atcatggttt attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc
    16021 ccatttcgac tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa
    16081 catgcccaaa ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag
    16141 cgattacaac aagatacgtt ccttttggga atgggatgat tcgggtagtg tttggaacat
    16201 cctgactcgt ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg
    16261 taaagaaata atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa
    16321 ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct
    16381 tcaattccac accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg
    16441 ccatttctcc caccctttct attatattaa gttcatctta cataaaatta aagggaaatg
    16501 acacgattac gctctttttt ctagggatat ttttattttt taactatttt tttgctttca
    16561 ctgcaatcca aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga
    16621 tctcgggagc ttcgactaac tcataatctg aagaaggaag ccattctgca tagattttcc
    16681 cccaagtatc ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt
    16741 gttcttctat agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc
    16801 caatcatgtg catcaaactg cccttttctt ccaagcgggc tccatcaaat ccataagaaa
    16861 cgttgattac ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct
    16921 ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa
    16981 cttttttcgc cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg
    17041 attttcctcc tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt
    17101 atctgctgat ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt
    17161 cgactgtttc atatccatat ctaaacgccg tttctgtcac tcttgatcca tctaggagat
    17221 cgcgattagc caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa
    17281 taaacgtaaa gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt
    17341 tataggaaag tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt
    17401 taagcatatt tatcttcctc cttgcttgcc tttttatcat attatcaaag aaagtcaatt
    17461 acttgatgaa tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa
    17521 aattctcaag cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc
    17581 tttagaaaaa taaattttgg ggaagacaca ttctaagtaa acaaagcggt aaaaatatct
    17641 tttattccct tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact
    17701 gctgatcaga acgctgcttg gatttttctt tgcgatacat catacctctt ttatcaattg
    17761 gaacaattat ttatttctaa caaaatcact tcatttttgt cagataaata aaa
//
LOCUS       DAJSBC010000051        17437 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSBC010000051 DAJSBC010000000
VERSION     DAJSBC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.051"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            231..470
                     /locus_tag="ONT65_001893"
     CDS             231..470
                     /locus_tag="ONT65_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329502.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(832..1092)
                     /locus_tag="ONT65_001894"
     CDS             complement(832..1092)
                     /locus_tag="ONT65_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329503.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1277..1774)
                     /locus_tag="ONT65_001895"
     CDS             complement(1277..1774)
                     /locus_tag="ONT65_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR2329504.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1904..2614)
                     /gene="budA"
                     /locus_tag="ONT65_001896"
     CDS             complement(1904..2614)
                     /gene="budA"
                     /locus_tag="ONT65_001896"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR2329505.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2627..4291)
                     /gene="alsS"
                     /locus_tag="ONT65_001897"
     CDS             complement(2627..4291)
                     /gene="alsS"
                     /locus_tag="ONT65_001897"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR2329506.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4499..5161)
                     /locus_tag="ONT65_001898"
     CDS             complement(4499..5161)
                     /locus_tag="ONT65_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2329507.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5171..5986)
                     /locus_tag="ONT65_001899"
     CDS             complement(5171..5986)
                     /locus_tag="ONT65_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2329508.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6247..6690)
                     /locus_tag="ONT65_001900"
     CDS             complement(6247..6690)
                     /locus_tag="ONT65_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR2329509.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6824..7162)
                     /locus_tag="ONT65_001901"
                     /pseudo
     CDS             complement(6824..7162)
                     /locus_tag="ONT65_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7150..7527)
                     /locus_tag="ONT65_001902"
     CDS             complement(7150..7527)
                     /locus_tag="ONT65_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2329510.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7751..8944
                     /locus_tag="ONT65_001903"
     CDS             7751..8944
                     /locus_tag="ONT65_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329511.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9110..9532)
                     /locus_tag="ONT65_001904"
     CDS             complement(9110..9532)
                     /locus_tag="ONT65_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329512.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10115..10609
                     /locus_tag="ONT65_001905"
     CDS             10115..10609
                     /locus_tag="ONT65_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329513.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10771..11943)
                     /locus_tag="ONT65_001906"
     CDS             complement(10771..11943)
                     /locus_tag="ONT65_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2329514.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12149..12964)
                     /locus_tag="ONT65_001907"
     CDS             complement(12149..12964)
                     /locus_tag="ONT65_001907"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2329515.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13114..13560)
                     /locus_tag="ONT65_001908"
     CDS             complement(13114..13560)
                     /locus_tag="ONT65_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2329516.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13557..14561)
                     /locus_tag="ONT65_001909"
     CDS             complement(13557..14561)
                     /locus_tag="ONT65_001909"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2329517.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14601..14930)
                     /locus_tag="ONT65_001910"
     CDS             complement(14601..14930)
                     /locus_tag="ONT65_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2329518.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15056..17383)
                     /locus_tag="ONT65_001911"
     CDS             complement(15056..17383)
                     /locus_tag="ONT65_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2329519.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5031 a         3458 c         3051 g         5897 t
ORIGIN      
        1 tcatacctac gttttcactt ataaattttt tcataaaaaa agcgtttaga ggcataatcg
       61 ttaatacaga tggtaaccaa tgtatctggt ttgaactggg atatcaccct ctccatatat
      121 gtattactaa gactaataga tactataaaa gacttgatag ttttgcattg attttgccta
      181 atactgagca taggtgtatg ctaaacgata ctataaggag tgtgtacata atgaaaagaa
      241 ttctgtttat cagtttatca aatttattgt tacttactgc ttgtgggact tctgcatcaa
      301 agaacatacc ttcagatacg caaatttcga atagtatcga ttcaacaatt tctacaaatg
      361 agaaaaattc tccggtagaa acttccgata taaaagtttc tacctctgca aaaaacaggg
      421 taaagaaact gtcagcacgt ctcctttatt tcaaacaaac tctgaattag cttcttcatc
      481 ttcttcagca aataatgatg atagtttcgc gtttactcct aactaaatgc aacctttaca
      541 aatgctttac gataacaagc ctgagctaaa tgatcctgat atcagttttg cattttttga
      601 aatgattgga tcggatgatc tttttaaaac tactagtttg tctatccgaa aataggacag
      661 ttctggcagt ataggttttt atcgagtttc tccaccaggc ctcgttacag agacagattc
      721 tctaggcgaa ccttttgaat aaatcaaaaa tcatgaacaa tcactgccaa ctaaataaag
      781 tgccttcaaa agcttggtct tctcgctttt gaaggcactt tatttaaatc tttatgaaac
      841 taaagtatgt tcatatttgt tgctatagtt atatagatat tctgttgttt ccaactcata
      901 aacgtgagta tattttgcca ctacttttcc gcatttttct aatatttcat tgcgatcgac
      961 gtcatgatat tcgtctacgc agatcacaac acaattttct aatttactga ttacttctcc
     1021 aaccactttt tgtttcaagc caataggttg gcaaatatat ttttttccta aaatgatagt
     1081 ttcgttattc atatttcatc catccttaat gttttttcat tattcaaaat gataatacca
     1141 ttaaaatatg attgtgaaaa tcaataacac ttgtgatttt ataaaaaaat aaaaaaaccg
     1201 aaaaacagaa atgaccactg tctttccggc tctttttatt cttgatttct cttctttttt
     1261 gtttcaatag acgattttag ttattaataa atgaaaaagc atttatatta aaatttgcta
     1321 ataaaagatc tacatatttt tctagaaatg cttgatcgat ttcatcatac cctttcgtta
     1381 ctccgcaatc gatatctaaa acaccgatta aattaccttc ttttaccatt ggcactacga
     1441 tttctgattg tgcggctgaa tcgcaggaaa tgtaatttgc atgggttttg acatcatcaa
     1501 cgatcaatgt ttcttgtttt tcagctgatt ctccacaaac tccttttccc atcttgatac
     1561 gtgtgcaaga aactcgccct tggaacggtc ctaaaatcaa ttcattacca tcgaataaat
     1621 aatatcctgc aaagacagtc tcaggcaaag tctcattcaa caatgcagat gaatttgcta
     1681 aatttgctaa ccagttatct tctgcttcta ataatccttg ttgctgtaat aataaaattt
     1741 cgtatgcatc ttttttctgt ttttctgtcc acatgataaa aaccgctcct ttttgatcta
     1801 tggagaagat tatagctcag tttttttgta aacgctaggt ttcctcctca aaaaaacgag
     1861 atacgtcagg ttaatcacct tgaacgcatc tcgctgtctt tttctattct gcttgttgga
     1921 tttctgctgc taaatcagca taatcaatcg tttcgtgaac aaaagtcgga tcatcaactg
     1981 gaaaatgctg tacaagggac gagatgcggc taatctcaac ttttccttca gccacttcaa
     2041 aatccattac atgtccgcca aatgtatgtg tatcatcgat aaaatgcagg tgaaatcccg
     2101 ctgcagcaac tccttggaat aattcaggcg taaagaaccc gactaatgtt ccttggatat
     2161 tgctttgttg gaattctggt tgagactctg aaatttttgc tagtcttctg taaggttttt
     2221 cttgcttcgg catgatacgg atgtgcatat ttttgaataa tccagttatc ttcactgcca
     2281 aaaataggtt ttccccggct tctcttacta atatgtcttt tttcaattgt tctccatctg
     2341 cagtttttga gacagaaaaa ctagtatctg gcgtaaaatc tgtaattgct gcataggggg
     2401 taagttcact tccatctaat tgggcaactg agccatctga tcttccttga taagctacgc
     2461 catctaaaaa gatgacttca ccatcaagac catgcaatgt tcccaatccc agagttcctt
     2521 tttctaatat atgagcaatc gtttctgttc catccatcaa gccggccatc aaagctccta
     2581 atgtcccgtg ctggtataag attttttcag tcatgtcgac acttccttaa tagaattgat
     2641 ctggtaagat tgtttctccc aatttctcat tatcacggta atcaatcgga atatcaataa
     2701 ttacgggtcc ttctgtcgca aatccttctt ctaaagcttt ttctaattcc tctacacttg
     2761 tagctcggat tcctttagcg ccaaaagctt ctgcatattt cacaaaatca actggaccaa
     2821 agtctacacc tgacgcccgc tgatatttca ttttttcttg gaactctacc atattgtagt
     2881 gtccatcatt ccaaatcaaa tgaacgatat tcaatttttt acgtaccgcc gtttctaaat
     2941 cctgggcaga gaataaaaat cccccatcgc cggaaactga tacgatttgt gtatttggac
     3001 gcaccaatgc agcagaaatc gcccatggca atgcaacacc aagcgtttgc ataccattac
     3061 tgaataacaa atgacgaggt tcataacttc taaagtggcg tgccatccaa atataatgac
     3121 tacctacatc tacggtaaca gtcatatcat ctgttacttt tgactgaagc gtgttaataa
     3181 cttctaaagg atgcaagata cctgcttctc cttttgattg aacaatgtcc cgctccgtta
     3241 attttgcttg aagagatgct aaatattctt ttgcatcttc tgacacttgt tgaggctcta
     3301 atgaacctgt caataaatca agagtggctg agatatcacc gatcaattct ctttcaggct
     3361 gcataaaagg atcgatttca gctggcgctt catcgatcac aatgatccgc gcatcttttt
     3421 ccgcattcca gtttcttgct tcatattcga ttggatcata gccaattgca ataactagat
     3481 cacttcgttt caaaagcata tctcctggtt ggtttctaaa taaacccaca cgaccaaaga
     3541 aatgcgcttc taattcacgt gagatcactc cagctgcttg gaatgtctct acaacaggca
     3601 atgctgtctt ttcaactaat tgcctgatgg ctaacgtttc tttttcactt gatcctcgca
     3661 tacctactaa tagtaccggt agttttgctt ctctaatgcg ttcagctagg taacggatat
     3721 catctgcaga agcagaacct aatttaggat cgctcaatgg cttgatgaca ttaccttgga
     3781 caggtgcatc cactacatct tgcgggatac tgataaagct cgcgcctttt tttgaactct
     3841 tagccatacg ataagcattg gcaatcactt ctgaaattgt ttctggatct tggatttctg
     3901 cactgtattt tgtgattggc tggaacagtg ctgcattatc catgctttga tgggtcaact
     3961 tcaatagatc gcttcgtttt acttgtccgg caattgctaa aactgggtca ccttctgctg
     4021 tcgctgttac caaacctgta gctaagttac tagcaccagg accacttgtt gcgataacta
     4081 ctcctggctc tcctgtaata cgtccgattg cttgtgccat aaatgcagca ttttgttcgt
     4141 gacgagtcac gattaattca ggaccttgat cctctaattc attaaataca ccatcgattt
     4201 ttgcacctgg tataccgaat acataatcaa catgatgatt aatcaaactt tctactacag
     4261 atttcgaacc ttgtttcatt ttttcagcca ttgaacatcg tcctttttat gctttatttt
     4321 taattattag ttagaaataa tcacaaataa gtgatacagt ttctaaacct gtttcataca
     4381 aaagtatagc acgttttatt tcatttgttt agccatatgt ttcaaaaaat gaaaaagaat
     4441 gacagttttt acataaaaaa aactgtcatt cttaattgaa agtttcaatt tataaagtct
     4501 acagatccat agttctcatc aatttacgat tgttgaagta aatcaaacca aatacgatag
     4561 aaggacctag tagaatacac cattggggct ttacgaaaaa gaagagaaac aaacttgtca
     4621 atagtaaaaa atctagtaaa ctcacatacc attttttcaa cgtatagtag aacgctatcc
     4681 gtagaacttg atttggtttc gacgctggat ttctcacctg aaaataccag cagttggtgc
     4741 taaaggaaac tgataaacaa gcaagtaaaa caaacaacgg aatcaaccat tttccataag
     4801 aaaaacgtat aaaaaagaaa caatcaaaaa tcattatcac actaactatc caagcaaata
     4861 gccaataaaa aaatcccttt agccaaaatt ggcgaaacat ctttatgaac aaacgtactg
     4921 gtgaaaaact attttctttt gctaattctt ctgtcatacc tatcatcacg atgcaacctg
     4981 gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct atcgacaaaa
     5041 ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta cttgccaatg
     5101 attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc tgctctttca
     5161 taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa tatttttgca
     5221 tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc atgattaatg
     5281 cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt gttttatttt
     5341 ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta acgaatgtga
     5401 tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg cgatagattc
     5461 cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg atactactgt
     5521 aatattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc accaacgacc
     5581 acaacgtatc tgttaaaccc acttttctca tcataatgaa ctgcgggatc atataagact
     5641 gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc cctttaaaat
     5701 ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc aacgtgatca
     5761 caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga gaccaaataa
     5821 ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc gtgaacactt
     5881 cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc gtcaataaag
     5941 ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt tttgaacgaa
     6001 tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca tttctttgta
     6061 atgattacaa tccagaacaa cggttattat agctgattca tttccgttct tcttattttt
     6121 gtaagcgctt atttaacaag ttatgtaaat ctctttattt ttttgattta aaaatgaaaa
     6181 aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga
     6241 tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga
     6301 taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg
     6361 aacgaatgtc actcgctttg aatgatgact tacagcacct tctatgacta aagcagcacc
     6421 tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag
     6481 caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt
     6541 tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa
     6601 aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc
     6661 gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag
     6721 tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc
     6781 acggatcaac ttttctagta atagtgccaa tgtattcgaa ctatcagctt ccacacagcc
     6841 gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg
     6901 attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca
     6961 agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttactct tctaactcct
     7021 tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg
     7081 ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc
     7141 ttgatttttt tacttgatac attccatttt atttctagat aacacacgat catatttctt
     7201 gatttttcct tgtaaacgtt ccaaacattc ttgcatttca gcaatttttt tcgtcaaaag
     7261 ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga
     7321 taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt
     7381 aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt
     7441 aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt
     7501 ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt
     7561 tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct
     7621 attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt
     7681 cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag
     7741 gggtataaaa atgacaacgt atcagcgaaa ggtattagta tcgacatcgg cgggaattgc
     7801 tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt
     7861 tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat
     7921 tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca
     7981 aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt
     8041 ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatatg gggcgtgtat
     8101 gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg
     8161 tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac
     8221 gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat
     8281 tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac
     8341 caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat
     8401 catggtaact gtccaaattg ccggctattt tgggttaatg aattggctac cttcaattat
     8461 gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt
     8521 aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact
     8581 agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca
     8641 tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta
     8701 tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa
     8761 taatttcgtt atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt
     8821 cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt
     8881 cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca
     8941 ttgatagttg agtctgtaaa tgacaagaaa gaggtgtgga aaaagcttgc atctgcggta
     9001 ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt
     9061 gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac tattctttga
     9121 cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac
     9181 tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt
     9241 caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg tagattggga
     9301 agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat
     9361 caggatattg ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg
     9421 ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta
     9481 aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc
     9541 ctcctgtatc tactttacta tactacatac aagaaaaaaa cggatacaaa tatcatattg
     9601 tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat
     9661 tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg
     9721 tacatatttt attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa
     9781 cacgggatta gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa
     9841 gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt
     9901 agtgaaaaaa ttagttgcgt tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc
     9961 tactacgtct actactcaaa gcacaagttc agctcaggct tccagcagca gtaccacgat
    10021 ctccgaaagt acggcagatt catcgaaaga acaaattgaa aagaaaaaag aagaagagaa
    10081 acaaaaagcg gaaaaagctg cactagaaaa aaaagtgaca gaagcggaat ctgctttgtc
    10141 acaagctgaa gcaaatccta caaaagaaaa ctatgcaact gcttcagcag ctatccaagc
    10201 gctacctgaa aacaaacaag agttgacaag ccgtcttgct gcaattgatt ccacaatcaa
    10261 agcaaaagaa gcagaagaag aacggaaaaa gcaagaagaa attgcacgac aacaagcaga
    10321 acaagcgcaa caagaagcaa tcgcacagca acaagcagaa caggcgaggc aagaagcaat
    10381 cgcacagcaa caagcagaac aggcgcgaca gcaagcagca gctgaacagg cttctcagca
    10441 ggcacaagcc gcagctcctg agcaaaataa tgaacaaact gtctatgtca caccgaccgg
    10501 ttcaaaatac catacacata aatgtggaaa tggtacgtat acccctgcca ctttaagcga
    10561 agctcaagct cgtggtttaa ccccttgctc caaatgttat ggaaactaat tcaaaagtta
    10621 tactataata ataaaattct tctgaaacat aaaaaaatag aagctggcgt tccagatagt
    10681 aaaccaaaaa tagcagttta cacgtccatt tcgtcagctt ctctcttttt tcatttagtc
    10741 tgttccctct gcttttttgg acatcttcat tcattctttc tcaatcaaat agtacacctc
    10801 tttttctttt cgcagtttat atcttccttt tttcaatttc attgaaaact gtttcgaatg
    10861 tttatgtttt acatggattt taagcaggag ctcctcatgt tgcaagcgat agttcctgcc
    10921 ttttaaatca gaaaatctca ctagtccgta ttcatcagaa gctttcacaa atggaacttt
    10981 ttctctagta atatagtgtt tgccgttgag cgtttttaca gaaaagacca ttccttcgat
    11041 tggtcgtccg tttgtgttct ttacataaaa agataaagga tagtttcgct tagcgatggc
    11101 caaagaatct aaataagcga tgagtagcca aactaagccg attgatccaa gtagtatacc
    11161 gataaaccaa ataaaaaatt tccatttttt ccattgatca attccttgga tcgctttttt
    11221 tgcctctttt gcttgataag gaatacgatg ccccgtcaca agcagtcgat gtgtattgac
    11281 catataagga gtgcaggtaa gcaaagtcac gagatctctg cctttttgga tacccaaaga
    11341 atctacttcg tcaggtaaga ccacttggat tttttctact tgatacgcca atgtctttcc
    11401 attgatctgg atgaaaaact ggtctccttt tttcaactta ggcaaatctg taaataattt
    11461 tgcttctggg agtccacgat gcccagaaag aacggcatgg gtgtttttcc cgccagtcgg
    11521 ataagaactt ccttccaata atgacgttcc tttttgcaaa aatatttccg ttgtctgatc
    11581 gaatataggt aagctgacat tgatttttgg gatttcgatg actccaagca tatgttccat
    11641 aaaaaaggca ttactaggta aatcttttaa tgcttttgca tcaactgctt ggttgaaact
    11701 gcctatccct ggtgatacgt tttgctctgc caactgttgg tttttctttg tcattttttc
    11761 ctgctgtttt tttatttcct ctgaattctt attcgatgct tgcttttgat aatggatgat
    11821 catttgctgg gcaagaaaat cattcagtga atcttctaca aaaggataag aaaagatccc
    11881 gatcccaacg actaaaatga ttgccatgaa gatgtccagt attcttattc tgcttttatt
    11941 catcatgtcc acctattttc taatatgtat atgaagaggt tgtgaaatca gcgccttgac
    12001 ctgagtatta gcgaagcaaa cggcaaaata gttcctccta ttttgccgtt tgttgagcga
    12061 ataccaagag accgaccact tgtcacaacc tttctcccca tttgaatcac tatcgtttat
    12121 ttatcgtatg atttttgttt ccaatagttt aagcttctgt ttcttgatgg cgttttttga
    12181 aatagatcac tgctcctcca actaaagcag tacctaccaa gatgaatacg atgatcccca
    12241 taccaccagt tgaaggcaag attcctttag gttggttatc gatcgttgtt tcatcagaag
    12301 ttgttggatc atcatctact gtaaattcag tgtacgcagc ttcattctta ggtaatgcgt
    12361 agccttctgg tgcttttgtt tcttgaaggc agtatgcccc ttgtttcagc ccttcgattg
    12421 aaaatttccc ttctgaatca gaaatcaatt tcacttcttc ccagtcagaa cctgtgactt
    12481 catctgacca agcatatggg tattctcctg aggcaaggtt tgctattttt ttcattaatt
    12541 taacagttgg atcttttgct cttagaatag aaaattctgc atcggctaat ttttcttgcg
    12601 tgttcacatc ttgtttgatg aagtggaaat caccgtagtt ttggacattt tctacagtaa
    12661 tcgtgttatc cgtagttgta ttttcccgat tgactacacc gtttggattc ccaagattgg
    12721 cttcatctgc gccatggatg ataccggttg tttgttgcaa ttttccgttt tttactataa
    12781 aagtagtttc tttttgtgga ttggttaatt gataaccatc tggtgctttt gtttcaacta
    12841 aagtataatt gccgtctatt aatccattga cactgatgga tccttcagta tcagttgtga
    12901 atgtcaacgc tgactcagca ggatcataga gagcgacacc agtgttttca tcaaaaccgg
    12961 tcacgaattg attctctgaa tttttgatct ggaattgcgc ccctgctaac ttatctaccc
    13021 ctgaaaagtt cccagctttc tggattttga tattgctttc tttgatgaca tttttagcat
    13081 ataggtgaat cgtgtctaaa ggtttgtcag ggtctagggt aagcttaaca cgatatttgg
    13141 cgacgtttcg acaccgggct ttggtgtttc tacaaagacg tatacagcat ctttcccatc
    13201 agatttacct ggaagagtca ggttagctaa tccttcacca gaagtcgtta cacttgcgaa
    13261 gggtgtagcg tctgctggaa ctgctgtgtc tttacttgga tagtacgtag cagtgatgtc
    13321 atatacgttg aattcaacgc cgttcaacgg ttcgctattc ggaaatgtca attccgttcc
    13381 gttgtttatt tgattttctg gtgctgaagt gaatttcaat ttatgcaaat taaccgtaac
    13441 attttctggt gtaggtgata cggctgcaga aacctctgca gttcctaggc cgagaacgaa
    13501 aggaagcagc atgatagcag ttcctaaaaa cgtccatatt tttgttttga tgtttttcat
    13561 tttatttccc tcctattgtt tcctttttgt tgttttttat aaaaccaagc tgtccctgtt
    13621 gaaaggacta gcaataaccc taaaattgag aaacctccaa gcatctcttc gcttgttttt
    13681 ggaaagcttc ctccattttt ttcatttgat ggaccatttg tctgcatctg aactggcgtt
    13741 tttccattat tcgtcggatt tgcttttggt tcgttgatga tttccaatgg tataggatga
    13801 agcggatcat aactgccttt ttcaacttca aagagaatgg ttgattgact caatatatat
    13861 cctttaggcg gtttaatctc cactagttcg taacttccaa aagacaatcc atggacagaa
    13921 aatgttccat ctttactgga agtaaaaatc gttgctcttg aatcatcttt ggctacccaa
    13981 tgatttatcc cgttttcttg cgctaagtat tggtcatttt ttttgactag aaattgagca
    14041 tctgctagtt tttgtttcgt ttgccgatcc actttgacaa attgtttgcc tcctgtttgg
    14101 atggcaagat gtgtttctac tgtttgattg tcagtcgtca aacgtacatc gttatcaaat
    14161 gtcgtttttt cactgttttc tttcaatgtc atttggtaac ggatcgttaa tttttttcca
    14221 gcaaacgaac taagatcttt tccagaatct gataagacaa aaccatgttt tattttttgt
    14281 gtgtgaaatg tatgaatctt gtctccacca atcaaaaaat cgatcgtttc tggttctagc
    14341 cataactgag gatcagctgt atctttgatc gtataggttc ctatctcgtt gatgttctca
    14401 ggaattgtta cggtgatgag ataagggact ttatccccat ctgcaaaatc tgtctgctta
    14461 ttctctactg tttttgttaa tgtgagtgca gcaggtattt tttgttcgct ctttggatac
    14521 aagtggatgg tcgtcaattt atgattagat gaatctgtca caggaagcac aacgacaaat
    14581 ggtgctgatt tttcttttac ttaatctgaa gtctttattt ccgtaaatcg gtaaacagcg
    14641 tcacgacctt gctcatcttt gtccgatgca ctaaaagaag caatgccctc ttcctcgtct
    14701 actgtttttg tcacattttc tgccagtatt ttttcactat cacttttttg tgctagtttt
    14761 ctttgtgcgt cttccacact acttccttct gaccgcagct tgtaaaattc atccgagaca
    14821 tcatatatag cgaaagtaac gccgttcaac ccttttactc cttgaaagtt atccgggata
    14881 tttattccgt cgttttgact gatacttggc atcttatcgc tttcgaacat tcgattatgc
    14941 aaagtaaatg taataggtga ttctctagct gacacagtcg gagaacctat aaaaaagacc
    15001 agtaggataa aaaagagaac agcaaatata tccacacgta cattttttgt ttttttcatc
    15061 gctgttcacc tgcttttttc ttacgatgga taaaataaaa gaagccagaa aaaataaaga
    15121 tcagtcctaa aaagacaaag ggcagtagac ctatcccacc tgtcgaagga agcaagagag
    15181 tcggattgat cgtcaactta gcggtatctg ttgcgataat cgtataatta ttgtttacac
    15241 ttccttcgcc taaactaatc gttactattt gtgatccaat atctgtccgg ctgttaactg
    15301 ttagggtata atccaatgat tcgcctaaga caacaccggt aacgtggaga tttccaaccg
    15361 aaatcgagtg actttgggca tcataattaa aggcagcgtc atcgacagta atcactgcgt
    15421 tagctttata gatggtataa gttcctgatg tgttggctat atcagtaaat gacaacttgg
    15481 gatttgctgc tttgactttg tctattccgc tattcgttag tttaattgta tagctgccag
    15541 cattaaccat attttttgct gattgtgcat ctgctgtgta atccatactg ctaagtgcca
    15601 cttgtactgg agtaagcgaa ctagtgacac cctctgctag cttgacatgg attgacggga
    15661 cacctgcttt tccatcatat gtcttactgc cactgagctc taccgcaccg ctgatttgct
    15721 taatcgtata gacatcagat gtctgcacga tagtaaataa atagtttgtt ctggcagcta
    15781 gtttagctag tccgcttttt gtcaattgcc aagacgtata ggttccaatt tcagtaggcg
    15841 cagttacttc aactcccttt tcattcatcg ctttgaaatc agttggattc catgtgattt
    15901 gatcaatact gagatcattg tttaaaaggg atgttgattc tgttttatca ttaaaggcat
    15961 atttaacgat cggtacaaca gaaagaccat catagtactt actgccattt gcattgattg
    16021 atactgttgc atttgtctta taatatagga tgacttcaga aatctgtttt ccatttgcgt
    16081 cctttacgat tacatttcca tttgtatcta aactagctaa aacactcatt gctgattggt
    16141 tctttgtttc tatcgctgaa acagctgtaa aggtatatcc gttgatagta ggcgtattag
    16201 tcgtcaggtc ttgtgcagca cttccgtaag taagattcat tattttagaa ggagcaatca
    16261 tctgtccaaa atagtcttga tacttaatag tgattggtgt ttgtttgcct gtataggtca
    16321 ccgtatcgac gttactttct agggctgtga aagaaccgtt tgtttgtgca aaagaatagc
    16381 ccgttactgc tgttggagtg ataaccactt tgtcacccac atggattcca gataatgtag
    16441 tcccgtacgt tccgattttt gctgctccac cattgactgt ataagaaaaa gttcctgtcc
    16501 gattcgcatt gacctgaacc gttgctgctt gataggttgg aaaatttgaa acatttccct
    16561 taccatcaac gatctgccca tttttgatcg agtaccccgt gaatggaata tcatttgcta
    16621 actgtatacc gattgaaaaa gtactcatac ctcctccggc aggaagccct ccccatgttg
    16681 agggtgtaaa agaataactt ttatccgcat tgggtgtaac tgaatcgatc gtatttccag
    16741 cggtagccac ccccatatag atactttttg ggaatgtgat agtagtacca tttactgtgg
    16801 agccaataaa cagattatgg atatctggac ggttatcaat aaaagcgatt gctttggtta
    16861 cgccttgtaa cgcagtttta tccgcactca tcgatccgtt aaccgcaaca atgactccat
    16921 ctttggcata tatcactgta tatccatctg gtatggtatg tccgttgaaa ttcgcagtgg
    16981 ccgcaacagt actaaatgtc tggcttctac ctgtttctga ataagctttc tcccctgctg
    17041 cttttgctgc tgtgatttct ttttctgtcg gatcaatcag ttgagtcgag gatgtggttg
    17101 aggttgtctc gttgttgcta gactgattgg tgctttctat ggactgtatt tcagaatagg
    17161 gtggactatt tgtctcctct gtcgtttggt tagcaaaatt tgacgtttct gtataaacgt
    17221 ttacataaat acttgataag ctccctaaga aagtaaaaat caagatgatc gaaatcataa
    17281 tgggatgtaa gtattttctt ttagatacag ctttgagtct agaaacaaaa tgtttttgac
    17341 atatacgttg gattttattt tgagttttct taatcatttt catccctcct ctcaataaaa
    17401 aatcatcaaa taatccttta cacgttattc taaattt
//
LOCUS       DAJSBC010000052        17227 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSBC010000052 DAJSBC010000000
VERSION     DAJSBC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.052"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..369
                     /locus_tag="ONT65_001912"
     CDS             1..369
                     /locus_tag="ONT65_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR2329520.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ONT65_001913"
     CDS             549..1490
                     /locus_tag="ONT65_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR2329521.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ONT65_001914"
     CDS             1559..2290
                     /locus_tag="ONT65_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR2329522.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ONT65_001915"
     CDS             2434..4614
                     /locus_tag="ONT65_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR2329523.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ONT65_001916"
     CDS             4634..5116
                     /locus_tag="ONT65_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR2329524.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ONT65_001917"
     CDS             5132..5893
                     /locus_tag="ONT65_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2329525.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="ONT65_001918"
     CDS             6024..7358
                     /locus_tag="ONT65_001918"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR2329526.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ONT65_001919"
     CDS             7665..8150
                     /locus_tag="ONT65_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR2329527.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ONT65_001920"
     CDS             8143..8826
                     /locus_tag="ONT65_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR2329528.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ONT65_001921"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ONT65_001921"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR2329529.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ONT65_001922"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ONT65_001922"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR2329530.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLCPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="ONT65_001923"
     CDS             12442..13071
                     /locus_tag="ONT65_001923"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR2329531.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ONT65_001924"
     CDS             13123..13455
                     /locus_tag="ONT65_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2329532.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT65_001925"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT65_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR2329533.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ONT65_001926"
     CDS             14281..15654
                     /locus_tag="ONT65_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2329534.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT65_001927"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT65_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR2329535.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ONT65_001928"
     CDS             16709..17149
                     /locus_tag="ONT65_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2329536.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5877 a         3008 c         3794 g         4548 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg tcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaat
//
LOCUS       DAJSBC010000053        16807 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSBC010000053 DAJSBC010000000
VERSION     DAJSBC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.053"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(157..864)
                     /locus_tag="ONT65_001929"
     CDS             complement(157..864)
                     /locus_tag="ONT65_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR2329537.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="ONT65_001930"
     CDS             complement(943..1281)
                     /locus_tag="ONT65_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR2329538.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="ONT65_001931"
     CDS             complement(1392..2354)
                     /locus_tag="ONT65_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR2329539.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="ONT65_001932"
     CDS             complement(2369..3700)
                     /locus_tag="ONT65_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2329540.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="ONT65_001933"
     CDS             complement(3708..4388)
                     /locus_tag="ONT65_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR2329541.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="ONT65_001934"
     CDS             complement(4607..5086)
                     /locus_tag="ONT65_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR2329542.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="ONT65_001935"
     CDS             complement(5213..6892)
                     /locus_tag="ONT65_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2329543.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="ONT65_001936"
     CDS             7440..8942
                     /locus_tag="ONT65_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329544.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="ONT65_001937"
     CDS             8955..9617
                     /locus_tag="ONT65_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329545.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="ONT65_001938"
     CDS             complement(9718..9921)
                     /locus_tag="ONT65_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2329546.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="ONT65_001939"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="ONT65_001939"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR2329547.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="ONT65_001940"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="ONT65_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2329548.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="ONT65_001941"
     CDS             complement(13885..14499)
                     /locus_tag="ONT65_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2329549.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="ONT65_001942"
     CDS             complement(15105..15482)
                     /locus_tag="ONT65_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329550.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="ONT65_001943"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="ONT65_001943"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2329551.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4872 a         3611 c         2868 g         5456 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cacttgctgt tcctcctcgt
    16681 aaaatatcat ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga
    16741 ataacacatt cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat
    16801 gtatgga
//
LOCUS       DAJSBC010000054        16607 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSBC010000054 DAJSBC010000000
VERSION     DAJSBC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.054"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..1819)
                     /locus_tag="ONT65_001944"
     CDS             complement(83..1819)
                     /locus_tag="ONT65_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR2329552.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1816..2520)
                     /locus_tag="ONT65_001945"
     CDS             complement(1816..2520)
                     /locus_tag="ONT65_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2329553.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2651..3154
                     /locus_tag="ONT65_001946"
     CDS             2651..3154
                     /locus_tag="ONT65_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR2329554.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3114..3452
                     /locus_tag="ONT65_001947"
     CDS             3114..3452
                     /locus_tag="ONT65_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR2329555.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3473..4891
                     /gene="ffh"
                     /locus_tag="ONT65_001948"
     CDS             3473..4891
                     /gene="ffh"
                     /locus_tag="ONT65_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR2329556.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5003..5581
                     /locus_tag="ONT65_001949"
     CDS             5003..5581
                     /locus_tag="ONT65_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR2329557.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5585..6106
                     /locus_tag="ONT65_001950"
     CDS             5585..6106
                     /locus_tag="ONT65_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2329558.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6185..6739)
                     /locus_tag="ONT65_001951"
     CDS             complement(6185..6739)
                     /locus_tag="ONT65_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2329559.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6992..7267
                     /gene="rpsP"
                     /locus_tag="ONT65_001952"
     CDS             6992..7267
                     /gene="rpsP"
                     /locus_tag="ONT65_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR2329560.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7279..7530
                     /locus_tag="ONT65_001953"
     CDS             7279..7530
                     /locus_tag="ONT65_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR2329561.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7655..8446
                     /locus_tag="ONT65_001954"
     CDS             7655..8446
                     /locus_tag="ONT65_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329562.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8616..9137
                     /gene="rimM"
                     /locus_tag="ONT65_001955"
     CDS             8616..9137
                     /gene="rimM"
                     /locus_tag="ONT65_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR2329563.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9127..9888
                     /gene="trmD"
                     /locus_tag="ONT65_001956"
     CDS             9127..9888
                     /gene="trmD"
                     /locus_tag="ONT65_001956"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR2329564.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10024..10371
                     /gene="rplS"
                     /locus_tag="ONT65_001957"
     CDS             10024..10371
                     /gene="rplS"
                     /locus_tag="ONT65_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR2329565.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10503..11639)
                     /locus_tag="ONT65_001958"
     CDS             complement(10503..11639)
                     /locus_tag="ONT65_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2329566.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(11755..12669)
                     /locus_tag="ONT65_001959"
     CDS             complement(11755..12669)
                     /locus_tag="ONT65_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2329567.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(12760..13188)
                     /locus_tag="ONT65_001960"
     CDS             complement(12760..13188)
                     /locus_tag="ONT65_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2329568.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(13193..13567)
                     /locus_tag="ONT65_001961"
     CDS             complement(13193..13567)
                     /locus_tag="ONT65_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2329569.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            13853..14038
                     /locus_tag="ONT65_001962"
     CDS             13853..14038
                     /locus_tag="ONT65_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2329570.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            14050..14298
                     /locus_tag="ONT65_001963"
     CDS             14050..14298
                     /locus_tag="ONT65_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329571.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            14329..14508
                     /locus_tag="ONT65_001964"
     CDS             14329..14508
                     /locus_tag="ONT65_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329572.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(14551..15021)
                     /locus_tag="ONT65_001965"
     CDS             complement(14551..15021)
                     /locus_tag="ONT65_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329573.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            15108..15806
                     /locus_tag="ONT65_001966"
     CDS             15108..15806
                     /locus_tag="ONT65_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2329574.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            15984..16325
                     /locus_tag="ONT65_001967"
     CDS             15984..16325
                     /locus_tag="ONT65_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329575.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            16318..>16607
                     /locus_tag="ONT65_001968"
     CDS             16318..>16607
                     /locus_tag="ONT65_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2329576.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         5465 a         2900 c         3247 g         4995 t
ORIGIN      
        1 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
       61 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
      121 ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa
      181 tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa
      241 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
      301 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
      361 attggacagc attttcaatt agatttttga taattggata aaggatatct gttttagtag
      421 taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct
      481 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
      541 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
      601 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
      661 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
      721 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
      781 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
      841 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
      901 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
      961 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     1021 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     1081 gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa
     1141 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     1201 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     1261 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     1321 cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta
     1381 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat
     1441 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     1501 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     1561 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     1621 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     1681 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     1741 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     1801 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     1861 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     1921 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     1981 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     2041 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     2101 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     2161 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     2221 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     2281 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     2341 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     2401 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     2461 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     2521 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     2581 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     2641 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     2701 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     2761 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     2821 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     2881 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     2941 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     3001 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     3061 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     3121 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     3181 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     3241 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     3301 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     3361 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     3421 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     3481 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     3541 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     3601 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     3661 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     3721 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     3781 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     3841 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     3901 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     3961 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     4021 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     4081 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     4141 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     4201 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     4261 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     4321 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     4381 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
     4441 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
     4501 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
     4561 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
     4621 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
     4681 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
     4741 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
     4801 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
     4861 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
     4921 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
     4981 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
     5041 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
     5101 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
     5161 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
     5221 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
     5281 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
     5341 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
     5401 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
     5461 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
     5521 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
     5581 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
     5641 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
     5701 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
     5761 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
     5821 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
     5881 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
     5941 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
     6001 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
     6061 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
     6121 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
     6181 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
     6241 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
     6301 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
     6361 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
     6421 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
     6481 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
     6541 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
     6601 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
     6661 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
     6721 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
     6781 aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat
     6841 tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt
     6901 aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt
     6961 gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat
     7021 gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga
     7081 tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt
     7141 tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt
     7201 acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa
     7261 gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg
     7321 ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag
     7381 tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt
     7441 gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt
     7501 ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg
     7561 cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa
     7621 gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc
     7681 atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct
     7741 atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc
     7801 caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt
     7861 gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct
     7921 caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg
     7981 tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata
     8041 tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga
     8101 cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc
     8161 aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa
     8221 tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa
     8281 cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa
     8341 tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga
     8401 tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga
     8461 tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt
     8521 ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta
     8581 aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa
     8641 aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc
     8701 cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg
     8761 tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag
     8821 gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag
     8881 acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg
     8941 tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg
     9001 atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg
     9061 tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga
     9121 ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg
     9181 gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc
     9241 cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca
     9301 ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca
     9361 ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa
     9421 atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga
     9481 tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt
     9541 actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat
     9601 gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat
     9661 ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat
     9721 ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa
     9781 cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa
     9841 gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa
     9901 tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa
     9961 ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa
    10021 accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg
    10081 ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt
    10141 atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat
    10201 acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca
    10261 cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac
    10321 ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcatcgtta
    10381 tcagactgag aaagaaccct tggtattcaa gggttctttt tttgttctct gtttttttat
    10441 attaacgatt tctatttggg acatttttga ttaataaaaa ggggcaaaaa aggggcaaca
    10501 agttacagtt tttctatatc ttctttcaat ttttctttta ctccctttgt cacatgcaaa
    10561 taaatctttt cagtgatatc actattttcg tgcccaacgc gatcctgaat agcatacaga
    10621 ggagttccta gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt
    10681 tttttatcaa tcttcatgtc agctttatga tttctcaaat aggtgttaat tgctgttagt
    10741 tggaaaggag ttcctttcgt tgtttgaaat agaaattgac cattcggatt taattctaga
    10801 agctcattgt aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccagcagca
    10861 gtttttgtag aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc
    10921 ccatttataa ctactgatgc attattattg gtaatgtgta catcgtcttt acttaatgcg
    10981 attgcttctc caggcctcat tccagtcaaa tatagccact ggaaaaggag agaatatctt
    11041 ttattgtgtg aagttgtgaa atttactagt cttttatatt catcatcttc taaaaactta
    11101 tctttaattt ttatcgtttt tgactctcgt ttataatcaa taactacttc gttgatagga
    11161 tttttttcta catagccttt tttcatagcg tatgaaaaaa gaagattcaa tttggattta
    11221 attacactga cgtacttatt tgacaagtca tctttatata tcatatcttc aaaaatatta
    11281 tttaaatcta ctgtattaat accagaaacg atgtaagtct caggtatctt tttctttata
    11341 gtgtttaaga tattattcgt aggataatat gtagactcct taacttgtct tttgtaaata
    11401 actaaccatt cttcgacaag ctcatgaaat gttagatcag gcttctgaag tgttttttcg
    11461 tttaatttta tatcaatttt tttatttaac tctagcaatg ccactttctg agtttctcga
    11521 gatttatttt tataagtaat gctcactttt tttctttttc tagtttttgg atcaatatat
    11581 cgctcattat atttatatac tttttttccg tttttatcag ttttggtttc aatccacatt
    11641 gttttcatct cctatttgtt gctataatag gaatggataa gtaagccaat tatagcaggt
    11701 ttatttttca tcacgtccac aaactttggc gagggagggg gcgtgttttt ttgtttagta
    11761 gctacctttc catgaagtat ctgcgaagaa cacttcaaat gttgttccat catttttcac
    11821 ttcaaaatat gctgttcctt gtgcggattt acccggttgg atggtttccg aaaagtaatc
    11881 tctactagct aaatcagatt taatatctga tgaatcataa aattctaaat aacttgaact
    11941 tgcatcaaaa ggtgtagtcc ctgtgttttc tacaagaaaa tctacctttg cgtaaaatag
    12001 gccttctggt ttatagaaat catcaccgta atcttttgat acagactgga cagttacgtt
    12061 aatagagtta ccattttcag aagtaaaagg aactgcttct cctatactat gtgcaactga
    12121 tgccgtctgg ggttctgaag acgaagttgt agttgtagtt gtagtagagc ttgaatcttt
    12181 ggattttcca tctttatcta agaaagttcg ttgattataa tatacaacct gttcagtttt
    12241 tggactaacc caaattaaca ttgtttcatc atcagaatca gttgaataag tatattggag
    12301 catctctaat ttttttatat cttttgcact atttccatgt tcaattaatt cagagtgctc
    12361 tttataataa tctccttgtg catcctttga aatagataca attaattcat cccgtgtgat
    12421 agcattgtct aattctttgt aaacaaaatc atttgtccat ttacgtaacg gtgaaccaag
    12481 tgtctgataa acttgagtta aatcatcacc aacttttatt ttatgaacgt ctgatgaagt
    12541 tagttttttt gtttcatatt cagatgttga tgaaacagta ttttctttag tgctagcaga
    12601 attaggacta gtggagcatc cgcttaatac caagcctaaa aaaagcaacc caaaaacaat
    12661 ttttttcatt tttaattcct cacttctatg atatgatttt tttgtaggat cttagaaata
    12721 aggtttttag tccgtgttgc agcacggact ttttttacat tatataagaa agcgataagc
    12781 gctttctgga agtccgtaaa gattctttaa ttcctcaatt cgtttaggat attgatcatt
    12841 gtcttctttg tatagagaaa caatgagatt agcagcaaag caattagctt cgctttcaga
    12901 tttgcttcta gatgttcttg ttgatacata gtaactggat aagccacgat gaaaaatagc
    12961 gtgacctaat tcgtgagcgc aaatgtagaa tctttcctca gagtctctca gttcatcatt
    13021 taagaagatt attgcacgac ctctaatttc ttgaaactgt cctttgggat tctcgataaa
    13081 aggaacgtat tgaattttaa tgcccatctt ttcacaaata taaaaaggat tagcggactg
    13141 gtatttccgc ttcaactcct cgactaaatt aatcgtatcc atctccataa gctcacatct
    13201 ttttgccttt ttctttgtct tctttcacaa tatcccagaa ggttccaata aggatatctt
    13261 ttacgcgttg gacctgttca ggcgtcagtg tttctccgcc ataagacata ttgacatttg
    13321 aatctagtaa tttatcaagt tcaactactt catcttttgt tgcccagtca ggaacttggt
    13381 ttctaccgag taaataatca gtagttacat taaaataatc tgctacaagt tgtagtcttt
    13441 tagtactagg agttctattt ttccattgat aaatcgtgtt tttaggtata tttaattctt
    13501 cttccaattg agttatactt atgcctcttt tatgagctaa ttcctttatt ctatctagta
    13561 aattcatttt cgcacctcac aagctacgaa aacactaata aaaaaattag ttaaaggtgt
    13621 tgacaactaa taaaagtgtt agtatactgt tttcgtaagc taaaatatta gctaaaaaga
    13681 cttgataact aataaacact tcacggtcgg caaactctga aatgtaaatt actaggcatt
    13741 tctgtgtctt atttagctat gtctatatac taataaaaat attagttatt gtcaacgaat
    13801 attagctaat tttttagctt acagattatt tttttagaaa ggagctattt ttatgtctga
    13861 gaatttagac ttaaaaattc gagcggagat gagaaaaaga agaatgactt tcaaagaact
    13921 agctgcgctt atcggtattt caggagctta tttatcagat attctaaacg gcaatcgtga
    13981 tggaaagaaa gcacaacagc atatcgaaac agtgaaaaaa atattggaca tccgataggg
    14041 gggtaggcga tgacaaaact aaaaaaacaa gattttgtaa aaaaatacaa ttattctcca
    14101 tctacttatc aacgtcgaat gtcggaacta aaaaatacag caattttctc agcggcgtat
    14161 gaacgggtca caggacaaga agtttggatc aatacagaat tatacgataa atttttgtct
    14221 ttcaaatcct ataacaggtt acgcacaaga aaggtaacac ctaaagaatt tatcgagaag
    14281 catttagttg atttataaaa aacagaacat tttgagaggt gaaggttaat gggtaaattc
    14341 aacagagcat tggtattcag cgcaccgcta atcatctacg ctttaggact ttggggaagc
    14401 aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa
    14461 gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa
    14521 aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta atttctctat
    14581 tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa
    14641 tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc
    14701 aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat
    14761 tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca
    14821 tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga
    14881 atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag
    14941 ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa
    15001 tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa
    15061 caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag
    15121 taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga
    15181 aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg
    15241 gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct
    15301 tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct
    15361 acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata
    15421 ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta
    15481 aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa
    15541 ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt
    15601 atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
    15661 caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg
    15721 aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga
    15781 gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct
    15841 aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa
    15901 aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac
    15961 acatcataag gagattttag catatggaaa aagaactttc cactctagat caatatttga
    16021 ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa
    16081 atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta
    16141 tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt
    16201 acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg
    16261 atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg
    16321 agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt
    16381 ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa
    16441 aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct
    16501 ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg
    16561 acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccga
//
LOCUS       DAJSBC010000055        16534 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSBC010000055 DAJSBC010000000
VERSION     DAJSBC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.055"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..546
                     /locus_tag="ONT65_001969"
     CDS             <1..546
                     /locus_tag="ONT65_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR2329577.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="ONT65_001970"
     CDS             735..1403
                     /locus_tag="ONT65_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR2329578.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="ONT65_001971"
     CDS             complement(1603..1989)
                     /locus_tag="ONT65_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329579.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="ONT65_001972"
     CDS             complement(1961..3343)
                     /locus_tag="ONT65_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2329580.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="ONT65_001973"
     CDS             complement(3499..4686)
                     /locus_tag="ONT65_001973"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR2329581.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="ONT65_001974"
     CDS             complement(4693..5724)
                     /locus_tag="ONT65_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2329582.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5740..7194)
                     /locus_tag="ONT65_001975"
     CDS             complement(5740..7194)
                     /locus_tag="ONT65_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329583.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7369..8274
                     /locus_tag="ONT65_001976"
     CDS             7369..8274
                     /locus_tag="ONT65_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2329584.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8365..9042
                     /locus_tag="ONT65_001977"
     CDS             8365..9042
                     /locus_tag="ONT65_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2329585.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9039..9209
                     /locus_tag="ONT65_001978"
     CDS             9039..9209
                     /locus_tag="ONT65_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329586.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9323..10219
                     /locus_tag="ONT65_001979"
     CDS             9323..10219
                     /locus_tag="ONT65_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2329587.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="ONT65_001980"
     CDS             complement(10274..11644)
                     /locus_tag="ONT65_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2329588.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="ONT65_001981"
     CDS             11882..12628
                     /locus_tag="ONT65_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2329589.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="ONT65_001982"
     CDS             12625..13464
                     /locus_tag="ONT65_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2329590.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="ONT65_001983"
     CDS             13465..14415
                     /locus_tag="ONT65_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2329591.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="ONT65_001984"
     CDS             14556..15308
                     /locus_tag="ONT65_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329592.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..16478
                     /gene="queG"
                     /locus_tag="ONT65_001985"
     CDS             15327..16478
                     /gene="queG"
                     /locus_tag="ONT65_001985"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR2329593.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5376 a         3095 c         3322 g         4741 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
     1141 tagagaatgc tggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaaca actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccagct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatacctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
     5761 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca attcctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagcgatg ctgatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa gtatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    16501 agtttttgtc cagtaattgt tgtaacaatt actg
//
LOCUS       DAJSBC010000056        16181 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSBC010000056 DAJSBC010000000
VERSION     DAJSBC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.056"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            11..325
                     /locus_tag="ONT65_001986"
     CDS             11..325
                     /locus_tag="ONT65_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329594.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(759..1125)
                     /gene="ssrA"
                     /locus_tag="ONT65_001987"
     tmRNA           complement(759..1125)
                     /gene="ssrA"
                     /locus_tag="ONT65_001987"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1296..2069)
                     /locus_tag="ONT65_001988"
     CDS             complement(1296..2069)
                     /locus_tag="ONT65_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2329595.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2213..2563
                     /locus_tag="ONT65_001989"
     CDS             2213..2563
                     /locus_tag="ONT65_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR2329596.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2544..3260
                     /locus_tag="ONT65_001990"
     CDS             2544..3260
                     /locus_tag="ONT65_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2329597.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3260..4708
                     /locus_tag="ONT65_001991"
     CDS             3260..4708
                     /locus_tag="ONT65_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2329598.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4778..5287)
                     /locus_tag="ONT65_001992"
     CDS             complement(4778..5287)
                     /locus_tag="ONT65_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329599.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5277..6002)
                     /locus_tag="ONT65_001993"
     CDS             complement(5277..6002)
                     /locus_tag="ONT65_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329600.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6225..8345)
                     /locus_tag="ONT65_001994"
     CDS             complement(6225..8345)
                     /locus_tag="ONT65_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2329601.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8575..9753
                     /locus_tag="ONT65_001995"
     CDS             8575..9753
                     /locus_tag="ONT65_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR2329602.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9769..10527
                     /gene="aroD"
                     /locus_tag="ONT65_001996"
     CDS             9769..10527
                     /gene="aroD"
                     /locus_tag="ONT65_001996"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR2329603.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10550..11035
                     /gene="ybaK"
                     /locus_tag="ONT65_001997"
     CDS             10550..11035
                     /gene="ybaK"
                     /locus_tag="ONT65_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR2329604.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11106..12359)
                     /locus_tag="ONT65_001998"
     CDS             complement(11106..12359)
                     /locus_tag="ONT65_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2329605.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12477..13826
                     /gene="gdhA"
                     /locus_tag="ONT65_001999"
     CDS             12477..13826
                     /gene="gdhA"
                     /locus_tag="ONT65_001999"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR2329606.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13938..15284
                     /locus_tag="ONT65_002000"
     CDS             13938..15284
                     /locus_tag="ONT65_002000"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR2329607.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15592..15978)
                     /locus_tag="ONT65_002001"
     CDS             complement(15592..15978)
                     /locus_tag="ONT65_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2329608.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(16027..>16181)
                     /locus_tag="ONT65_002002"
     CDS             complement(16027..>16181)
                     /locus_tag="ONT65_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2329609.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT         5283 a         2866 c         3234 g         4798 t
ORIGIN      
        1 aggatagctt ttggattttt acagtaattt tatcttaata atagcaatat tattacttct
       61 aaatatttgg ttttttgata agagtagaaa tgcagggata ggttttagaa ccaaacgaag
      121 tacgtcttct gaaaaaaagt gggtatattc acagactatt ttttacggag gggttatttc
      181 aatcagttta ctttcttcaa cactttattc ttttaatgtt atagacgttt ccatgtcaaa
      241 ttttatatct attattggaa tccttatttc tgcaattatt acacaactac ttcttgtgtt
      301 tgaagagaag tcaaaaaata attaaagcaa ttatgccttt tagtacagga attattactt
      361 cttacaaatg tatagcgaat gtgaagcctg ctgttttaat tgttctagaa aaacttttga
      421 gtgaacaatt attttatgtt aggatgaatt aatttctcta gagtttataa ttaaaaaact
      481 tatttgaagg aacatgctta aaccataatt gtgtttttcc ttccatcagc ataaaagttc
      541 acctcaaaaa gagtgtacga acaaaagttc tttttgtaaa ccttaaaata gattttcctg
      601 aatacgtatt ggggatttct aaatgtttga atgttttttt gaatgcaaaa tcaatgcaaa
      661 aaattttgta tttctctact tttctctcct tgaccaaaat aaaaaaacgt tgatttaaca
      721 acgtttttca tacatgttta tctgtctcaa ccatacatat atggagacgg cgggagtcga
      781 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
      841 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
      901 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
      961 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
     1021 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
     1081 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
     1141 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
     1201 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
     1261 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
     1321 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
     1381 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
     1441 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
     1501 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
     1561 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
     1621 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
     1681 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     1741 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     1801 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     1861 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     1921 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     1981 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     2041 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     2101 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     2161 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     2221 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     2281 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     2341 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     2401 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     2461 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     2521 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     2581 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     2641 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     2701 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     2761 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     2821 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     2881 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagcagttt
     2941 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     3001 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     3061 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     3121 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     3181 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     3241 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tttgcggaat
     3301 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     3361 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     3421 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     3481 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     3541 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     3601 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     3661 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     3721 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     3781 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     3841 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     3901 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     3961 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     4021 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     4081 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     4141 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     4201 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     4261 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     4321 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     4381 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     4441 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     4501 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     4561 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     4621 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     4681 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     4741 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4801 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4861 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4921 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4981 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     5041 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     5101 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     5161 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     5221 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     5281 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     5341 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     5401 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     5461 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     5521 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     5581 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     5641 aaaaggaata atttggtttt agaaagacca gtcaggcgcc aattgatcaa ttcctgcttg
     5701 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     5761 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     5821 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     5881 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     5941 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     6001 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     6061 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     6121 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     6181 gcagcgacaa taattttgtc gctgctcctt ttaaatttat tttgtcattc ggaagaactt
     6241 gtggtcgttc cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac agttgattgt
     6301 ccgttgtgtt cactaaagac actagttgac ttgtcaccaa gagatttttc agcttctttt
     6361 aatttctcta agccattctt gtagtcataa tcactggtat caattgggtc taatccacta
     6421 ttcgtataga accgaagaag atcaccatta ttgatttggt cagaaacagc taactgttta
     6481 tcaacttttt ctttcagtga atcaatttca ttctgtactt cctcagtagg ttcagtaata
     6541 gcttcaccag tttcgttact atagatcgta ccaccgtagt aagtatattt tggtgtaaca
     6601 aaatcaccat cacggaaaga aacaagctgt tcatgatctt tcgagaatag atcttgtcct
     6661 aactgaatat aattttttgt atctacacct agtaagtgaa gtaatgtagg caaggcatca
     6721 acttgtccgc cgtaagtgtg attgattccg ccctgtgttt gtccagggat atggatcata
     6781 taagggacac gctgcatttg tgcattgtcg tagtttgtcc aagtagaact tgtttttcca
     6841 actaattcag ctagattttt atttcttgaa ttcgagacac cgtaatggtc accgtaaagg
     6901 acgatgactg aattatcata caagccgctt tctttcaaat aattgaagaa ttcttgtacg
     6961 gcggtatcta aataattggc tgtcgcaaag taaccgttga tcgtttcatc atttgtattg
     7021 gctaacggga atcctgcttc gtcattggtg aattttgaat aaggataatg gttagataca
     7081 gcaatgaatt ttgaataaaa tggttgttgc agacgttcca agtattgtac ggattgttgg
     7141 aaaaatggtt tatcatgcaa cccgtattgg aacgaattgt catcattaac atcataataa
     7201 gaagaatcaa agaaataatc atatcctaaa ttcttgtatg tctcgtttcg attccagaat
     7261 gtaccagcgt tcccatggaa taccgcacta gtgtaacctt gtgtttgact taaaatatca
     7321 ggtgcagctt ggaaagtatt ttttccgcct aattgtgtga acaaggagcc ttgattcaag
     7381 ccaaacaatg aattttccat cagcgtttcc gcatcgcttg tttttcctgc ttttacttga
     7441 tggaaaaagt tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt gatgaaaggt
     7501 gtaacttcat gctggagacc attctcatct gtcaatttat aatcgatcag gaattgctgg
     7561 aagctttcta aatggatata aatcacattt tttcctttag cgagcccgaa caaatcgtca
     7621 tttggtgctg cataatgttc ttttacataa ttttctactt ctttcatatc attgggactt
     7681 gcttctgccc gcacttggtt tgtctggtaa gtttgaacac cgtcataaac catgaaagca
     7741 ttcaagccaa gatatttgac caaataatca cgagagaatg ttcgagtcag caattctgga
     7801 cgatcagctt ccgccataaa aagatttcct gagaaaatca taactgctaa tgtagagatc
     7861 gcaaaagcca ttcttgctcg gatcgggcga gggtccaact tgattttttt tgcaatcagc
     7921 agaacagtca agataataaa atcgatccaa tagatcacat catatgggcg aaacagtcga
     7981 atggcacttt ctcccagacc actcgctact ttcccagcac ctaaaatcgt gttgatcgta
     8041 atgaagtccg tgaattctcg ataataagtg acattcgaaa aaacaagcaa tgtcaaaaga
     8101 aaataaatga ccatcatcgt gatataagca actctagggc gtctgatata aagagcaatt
     8161 ccaagtaata acaatgtagt tgcaataggg ttgataaaca agataaagta ttgaatgcca
     8221 ctttcgattc cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa ccaaagtaaa
     8281 acgactagta aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt ttttatacat
     8341 ttcaagataa tcttcctttc tgcacttctt aatcacagaa gtgtcatgaa accatgttac
     8401 acagctcact tattctcatt tattttacta gtatgacaat atagtgtcaa ttgaaaccag
     8461 aagtttatgc tgtttcatta aggttttctt taagcttcat gaaaaaatga gcagtaagac
     8521 aagtcctatg ttttttgtta tacttgggaa gagaaagcta aagcaggtga aggaatgaaa
     8581 gtaaaagtaa aaaaaagagc aagcaatcga ttgaaaaaag gatatccact gatccaaaaa
     8641 gaagaccttg ctcatcccca aaaagaaacc acgcaatggg tggaattagt tgattcgcaa
     8701 aaccaattta tcgcaaaagg atatctagga aagcagaata agggaatcgg ttgggtattg
     8761 agccagcaga acatcccatt tgatcaatct ttttttgaag aattttttgt gaagtcaaaa
     8821 gaaaaaagaa ctttcttttt ttcagatgat tctactaccg cttttcgtat attcaatgga
     8881 gaaggagacg gaatcggcgg tctgattgtt gattattatg caggttatgc tgttttttct
     8941 tggtataacg aaacgttata tgcacatcgt gaattattga ttcaagcttt tcgtgaaatc
     9001 tacccggaaa tcgtaggagg ctatgagaaa attcgatttg aatcagtaaa tctgcctgag
     9061 agccagttca tttacggaaa agaggcaccg gaacccttga tcgtaaaaga aaacggagta
     9121 aaatttgccg tttatttaaa tgaaggatta atgactggga tctttttaga ccaaaaagaa
     9181 gtacggggat cattagcaga aggtttcgct tctgggaaaa cagttttgaa catgtttagt
     9241 tataccggcg ccttttctgt tgctgccgca atgggagggg caatcgaaac aacaagtgtc
     9301 gatctagcaa aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa tggtttggat
     9361 gtcgatcagc aaaaaatcat tgttatggac gtattcgagt atttcaaata cgccaaaaga
     9421 aaacaatttt tatatgacat gattgtattg gatccaccaa gttttgccag aaataagaaa
     9481 aaagtctttc gcgtagcaaa agattatggg gaactggtca aggatagtct ggatatttta
     9541 gcagaagacg ggatacttat tgcgtctaca aatgctgcga atgtctcgat agaacaattc
     9601 caaacaatga ttgaagagga attccatgcc aaaaatgttt cttttgaaca acttgcttta
     9661 tatcgtctgc caaaagattt ccagacgacg gatacctttt tagaaggcaa ttatctgaaa
     9721 gtgtttgttt atcaaattaa aaataaccat tagaaggagc gatgcagaat ggtttgggta
     9781 aagggattga atataggaag cggtagacca aagatttgtg cgcctcttat cggggccgtc
     9841 cgagaggaag tattacgtga agcagttctt gcacgtgaag caggggccga tttagtcgaa
     9901 tggcgagtcg atcattatcg tgaagttttt tcttttgatg aagtagcaga aacattggtt
     9961 cttatccatg agatgcttga tggactgccg ttattattta cgtttcgtac cttagctgaa
    10021 ggcggagaac aagacatatc gatccgtcag tatcgcgacc tttatgaaca tgtgatacac
    10081 acaggattag tagatatggt cgatatcgag ttgttcaaag tagaaagtct aggaaaaaga
    10141 ctgatagagg aaatcaagtc tttaaacatt cctttaatta tcagtagtca tgattttaaa
    10201 gaaacaccag cagaccctgt cttgctttac cgattgaatg ttatggaaca ttttggagcc
    10261 gatatcggaa aactggcagt aactcccaat aatgaacgag atgttttgcg gttgatggag
    10321 ttgactagac gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc catgggaaat
    10381 cttggtaaaa tttctcgtct ggctggcgga acgacgggtt ccgttatgac atttggttcc
    10441 ttaagcagcg attctacttc tgctccagga caattaccgg tcaaagcatt gaagcaaatt
    10501 attcaattat taaatgaacc gttgtagaac aagtttgagg tgatattttt tggcaaagaa
    10561 aaaaatagta aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc cctatacaga
    10621 gcacgaatat gagtgggacg aaagccacct aagtgcgtca tcggtcgctg aacagcttcc
    10681 agaaagccaa tctcgtatat ttaaaacact ggttgctgtg ggcaatgtga cggggcctct
    10741 tgttgctgtg ataccgggag aagccgaatt gaatctaaaa aaattagcaa aagtttcagg
    10801 gaataaaaaa gtagaaatgc tccatttaaa agatttggaa gcaacgaccg gatacatacg
    10861 tggtggctgc tcgcctatag gtatgaaaaa actatttcct acttatttag atcaaattgc
    10921 agaaagctat gaacaaatca ttgtctcggc tggcagaaga gggctacaga tggagttagc
    10981 acctcaagat atctgtgcat taacgagcgg acaatttgca gatataaaac aataaatcat
    11041 acgtataaaa gaaagaagga ggccgggatg ttgtttgtcc agtctccttc tttctttttt
    11101 atacattatg ctctttgccc ggcattcatt cctgctacat gccctgtagc gaaagccgct
    11161 gtgatattaa agccgccagt ataaccattg acatccagta attctcccgc aaaatacaga
    11221 ccatcaatga ttttactctc caatgtttta ggataaattt cctttagaga aacccctccg
    11281 ccagttacaa atgatttctc aataggaaaa gttttgttga ttgatagctt aaactctttg
    11341 catagttgaa caaaatcttg aatttgtttt tgagaggctt gttgtcctgt cagatgatcc
    11401 atttcaagcc gctcaagaaa gaaaactaac aatcgctcag gcaatatacc atgccaagca
    11461 ttgaccaagt tctttttggt tgctttactt ttttctgtca atatatgaat aagctcttgt
    11521 ttggtttgtg tagggaaaca atccagagaa agtgtcactg gttctcccgt tttctctaat
    11581 tctttattga caaaacttga acaacgcagt gcagccggac cagaaatacc gaaatgggtg
    11641 aaaagtaaat ccatcgtatg ttcagtcaat acacgacctt ttttatttaa aacgcgtaaa
    11701 gtgatgtctt gtaaagaaag cccttgaagt gttttctcct gaatatacgg ttcatccgat
    11761 atcaaggggg actctgtcgc ataaagaggc gtcaccgtgt gacccactcg cttcacgatc
    11821 ttgtacccat ctccagtaga accagtagat ggataagtcc gtccaccagt tgttaagata
    11881 atggcaggag catggaactc ttcgaattcc gtgcgtatac cgtaaatttt atgttctttg
    11941 tggatcaatt tctctactcg agtagagaat agaagcgtca catctagttc tttcagacga
    12001 ttgagcaatg cttcgataat cgtttttgat ttatttgtta caggaaacat ccgtccatga
    12061 tcctcttctt ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc gaaattattc
    12121 cattgcgcaa acgtactata taaaaacttt ccatttccag gaatatgtgc aatcaaatca
    12181 tcaacaggac gattgttcgt aacattgcac cgaccaccac ctgtcatcag tagttttttt
    12241 cctgcttttt tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc tgcactgatc
    12301 gctgccatca tcccgcttgt tccagcgccg actacgatca catcatacag tttttccatt
    12361 ccctcacctc aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa aaatgttttg
    12421 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
    12481 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
    12541 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
    12601 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
    12661 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
    12721 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
    12781 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
    12841 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
    12901 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
    12961 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
    13021 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
    13081 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
    13141 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
    13201 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
    13261 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
    13321 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
    13381 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
    13441 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
    13501 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
    13561 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
    13621 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
    13681 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
    13741 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
    13801 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
    13861 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
    13921 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
    13981 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
    14041 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
    14101 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
    14161 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
    14221 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
    14281 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
    14341 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
    14401 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
    14461 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
    14521 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
    14581 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
    14641 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
    14701 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
    14761 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
    14821 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
    14881 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
    14941 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
    15001 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
    15061 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
    15121 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
    15181 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
    15241 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
    15301 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
    15361 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
    15421 cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga
    15481 ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca
    15541 gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag
    15601 gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat
    15661 gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt
    15721 ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc
    15781 ataagcatct acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat
    15841 atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt
    15901 agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa
    15961 ccctaaaatt tttttcatga aagatctcct tttttatttt tattataccc gtggtgctag
    16021 ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact
    16081 taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc ctttaacaga
    16141 acgtgagttg aaattttgac aacctaattt ttctaaggaa g
//
LOCUS       DAJSBC010000057        16045 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSBC010000057 DAJSBC010000000
VERSION     DAJSBC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.057"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="ONT65_002003"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="ONT65_002003"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR2329610.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="ONT65_002004"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="ONT65_002004"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR2329611.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="ONT65_002005"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="ONT65_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR2329612.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="ONT65_002006"
     CDS             complement(4503..5270)
                     /locus_tag="ONT65_002006"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR2329613.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="ONT65_002007"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="ONT65_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR2329614.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            6383..6781
                     /locus_tag="ONT65_002008"
     CDS             6383..6781
                     /locus_tag="ONT65_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2329615.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            7060..7560
                     /locus_tag="ONT65_002009"
     CDS             7060..7560
                     /locus_tag="ONT65_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2329616.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="ONT65_002010"
     CDS             complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="ONT65_002010"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2329617.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8346..8573)
                     /locus_tag="ONT65_002011"
     CDS             complement(8346..8573)
                     /locus_tag="ONT65_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR2329618.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="ONT65_002012"
     CDS             complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="ONT65_002012"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2329619.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(9108..9821)
                     /locus_tag="ONT65_002013"
     CDS             complement(9108..9821)
                     /locus_tag="ONT65_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR2329620.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(9824..10720)
                     /locus_tag="ONT65_002014"
     CDS             complement(9824..10720)
                     /locus_tag="ONT65_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2329621.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(10878..11720)
                     /locus_tag="ONT65_002015"
     CDS             complement(10878..11720)
                     /locus_tag="ONT65_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2329622.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11852..12505
                     /locus_tag="ONT65_002016"
     CDS             11852..12505
                     /locus_tag="ONT65_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR2329623.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(12568..13095)
                     /locus_tag="ONT65_002017"
     CDS             complement(12568..13095)
                     /locus_tag="ONT65_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2329624.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13115..14062)
                     /locus_tag="ONT65_002018"
     CDS             complement(13115..14062)
                     /locus_tag="ONT65_002018"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR2329625.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(14074..15960)
                     /locus_tag="ONT65_002019"
     CDS             complement(14074..15960)
                     /locus_tag="ONT65_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2329626.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         4222 a         3323 c         2809 g         5691 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccgatgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
     6541 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
     6601 tgctttgatt tcgtttagtg ttgaaaacgc ggatgaagta aaaaaatttg ctcaaattgc
     6661 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
     6721 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
     6781 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
     6841 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
     6901 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
     6961 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
     7021 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
     7081 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
     7141 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
     7201 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
     7261 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
     7321 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
     7381 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
     7441 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
     7501 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
     7561 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
     7621 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
     7681 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
     7741 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
     7801 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
     7861 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
     7921 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
     7981 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
     8041 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
     8101 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
     8161 agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt
     8221 tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa
     8281 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
     8341 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
     8401 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
     8461 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
     8521 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
     8581 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
     8641 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
     8701 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
     8761 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
     8821 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
     8881 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
     8941 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
     9001 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
     9061 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
     9121 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
     9181 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
     9241 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     9301 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     9361 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     9421 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     9481 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     9541 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     9601 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     9661 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     9721 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     9781 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     9841 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     9901 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     9961 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
    10021 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
    10081 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
    10141 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
    10201 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
    10261 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
    10321 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
    10381 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
    10441 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
    10501 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
    10561 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
    10621 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
    10681 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
    10741 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
    10801 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
    10861 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
    10921 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
    10981 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
    11041 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
    11101 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
    11161 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
    11221 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
    11281 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
    11341 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
    11401 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
    11461 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
    11521 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
    11581 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
    11641 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
    11701 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
    11761 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
    11821 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
    11881 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
    11941 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
    12001 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
    12061 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
    12121 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
    12181 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
    12241 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
    12301 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
    12361 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
    12421 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
    12481 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
    12541 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
    12601 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
    12661 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
    12721 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
    12781 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
    12841 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
    12901 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
    12961 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
    13021 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
    13081 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
    13141 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
    13201 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
    13261 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
    13321 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
    13381 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
    13441 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
    13501 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
    13561 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
    13621 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
    13681 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
    13741 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
    13801 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
    13861 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
    13921 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
    13981 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
    14041 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
    14101 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
    14161 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
    14221 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
    14281 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
    14341 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
    14401 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
    14461 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
    14521 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
    14581 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
    14641 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
    14701 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
    14761 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
    14821 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
    14881 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
    14941 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
    15001 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
    15061 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
    15121 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
    15181 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
    15241 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
    15301 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
    15361 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
    15421 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
    15481 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
    15541 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
    15601 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
    15661 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
    15721 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
    15781 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
    15841 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
    15901 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
    15961 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
    16021 ggtatttgtt ctttttgtgt tctca
//
LOCUS       DAJSBC010000058        15983 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSBC010000058 DAJSBC010000000
VERSION     DAJSBC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.058"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..1475
                     /locus_tag="ONT65_002020"
     CDS             138..1475
                     /locus_tag="ONT65_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR2329627.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ONT65_002021"
     CDS             1523..3652
                     /locus_tag="ONT65_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR2329628.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ONT65_002022"
     CDS             3627..4316
                     /locus_tag="ONT65_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329629.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ONT65_002023"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ONT65_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5713)
                     /locus_tag="ONT65_002024"
     CDS             complement(5546..5713)
                     /locus_tag="ONT65_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329630.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(5792..6232)
                     /locus_tag="ONT65_002025"
     CDS             complement(5792..6232)
                     /locus_tag="ONT65_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR2329631.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6236..7081)
                     /locus_tag="ONT65_002026"
     CDS             complement(6236..7081)
                     /locus_tag="ONT65_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR2329632.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7230..8648
                     /gene="pepV"
                     /locus_tag="ONT65_002027"
     CDS             7230..8648
                     /gene="pepV"
                     /locus_tag="ONT65_002027"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR2329633.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8705..9652)
                     /locus_tag="ONT65_002028"
     CDS             complement(8705..9652)
                     /locus_tag="ONT65_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2329634.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9875..10225)
                     /locus_tag="ONT65_002029"
     CDS             complement(9875..10225)
                     /locus_tag="ONT65_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2329635.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10425..11504
                     /gene="pepA"
                     /locus_tag="ONT65_002030"
     CDS             10425..11504
                     /gene="pepA"
                     /locus_tag="ONT65_002030"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR2329636.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11648..11968
                     /locus_tag="ONT65_002031"
     CDS             11648..11968
                     /locus_tag="ONT65_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2329637.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11990..12454
                     /locus_tag="ONT65_002032"
     CDS             11990..12454
                     /locus_tag="ONT65_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2329638.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12659..13264
                     /locus_tag="ONT65_002033"
     CDS             12659..13264
                     /locus_tag="ONT65_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR2329639.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13303..14592)
                     /locus_tag="ONT65_002034"
     CDS             complement(13303..14592)
                     /locus_tag="ONT65_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR2329640.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT65_002035"
     CDS             15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT65_002035"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR2329641.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15702..15983
                     /locus_tag="ONT65_002036"
                     /pseudo
     CDS             15702..15983
                     /locus_tag="ONT65_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5038 a         2942 c         3258 g         4745 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catatgggga tcacctgctt ttttcttttc attatacgaa ctactcgaca
     5761 caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc aaagaagcgt
     5821 acgcttggaa taactgatct aactccggat tgactttgct cagcttcact aatcgttgcg
     5881 aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt ttgtcttcag
     5941 ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg atagttggat
     6001 ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt acttccagaa
     6061 cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga tgtaccaata
     6121 aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata atccccattt
     6181 gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc atgactcatg
     6241 cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt gcttcgctga
     6301 tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg aaactatgaa
     6361 gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga
     6421 tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa cctaaaggat
     6481 tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa acgagcacag
     6541 caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt atcggtagag
     6601 aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg ggatagggta
     6661 gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc agccattgtt
     6721 cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa gaagtaccca
     6781 atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac tggtcagtat
     6841 ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc cgattttttt
     6901 catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa gcactcataa
     6961 tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca aatgttcctt
     7021 tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg tttatcagca
     7081 tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa acagataaga
     7141 aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat catgaacacg
     7201 aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa gaagtagaag
     7261 cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac agcgagcgcg
     7321 atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac gcattgaaac
     7381 atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag
     7441 ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac atggatgtcg
     7501 ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag gatggaaaga
     7561 tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac tatgcgatga
     7621 agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg
     7681 atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa gaaccagatt
     7741 ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt
     7801 tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct tttgtttccg
     7861 gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta ccaagtgcag
     7921 atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat ccagtaagcg
     7981 gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg
     8041 gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc ttagataact
     8101 atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat gtccatgaag
     8161 atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc aaattgacaa
     8221 tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac ttcatcaata
     8281 tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa
     8341 cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa ccacattacg
     8401 taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac cacactggag
     8461 aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg aaacgtggcg
     8521 ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca aacgaattta
     8581 tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc tatcgtttgg
     8641 cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag tcttgatcct
     8701 ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat tcatattctt
     8761 tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta
     8821 aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca
     8881 tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag aagatggtcc
     8941 aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc atcatgatca
     9001 ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga cgatcacgat
     9061 aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc gtcaattccg
     9121 tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata cgattgaatc
     9181 ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta attgtatttc
     9241 taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag taggttccat
     9301 ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc tcgagcaaac
     9361 catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa tagtcggcat
     9421 cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt tcttttttta
     9481 cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga
     9541 cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt gaaattgttg
     9601 cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc atttttcact
     9661 ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa aggaaatgct
     9721 agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata tgtttggaaa
     9781 gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct tttgaatagt
     9841 gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct ttttttcttg
     9901 aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa acagtttcgc
     9961 catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg aatttcctaa
    10021 taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt agaaaagatg
    10081 acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga taccaaatcg
    10141 ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg cctttaaaat
    10201 agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact tgttactcct
    10261 tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct accagaaaaa
    10321 ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc gcagatcaat
    10381 ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa gaaaaaacat
    10441 ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa caagatgttc
    10501 gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta gatggtttgg
    10561 gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta atggtcgcag
    10621 cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg ctttttaaag
    10681 tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt acacttaaaa
    10741 cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat ttattgcgag
    10801 gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct gggtttgaat
    10861 caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg
    10921 aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac aaccgctatg
    10981 gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta ggtcatacat
    11041 tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa gcttctgtca
    11101 ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat gatacagtga
    11161 cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc caagatccag
    11221 gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa acaaacaata
    11281 tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct catacacaaa
    11341 acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat acgcatcaaa
    11401 cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa acactgaaag
    11461 gtttggataa atcaacagta aatacgatcg tatacggaaa ataatgcgat agtgaaacta
    11521 taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag gatcaatgtt
    11581 tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag aaagtagagg
    11641 acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag aaactggaaa
    11701 gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca aaccgcaaat
    11761 gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc gtgacaagta
    11821 catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg tgatccaaga
    11881 cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc
    11941 gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga tgaatcaaga
    12001 atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa
    12061 agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg taatcgatca
    12121 gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg atcagcaaac
    12181 tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg ttgattacac
    12241 aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag tcaaattgcc
    12301 aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg cagtcgaacg
    12361 ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg atgtcttgat
    12421 cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta cgaccttttt
    12481 aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta
    12541 cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa aaatttcatg
    12601 cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat
    12661 gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta tagccgacga
    12721 taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca aagatgaaca
    12781 aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa tcgaagggaa
    12841 cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga tcaaagaagc
    12901 aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa
    12961 aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag aagtagacaa
    13021 tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac aaaaagttgt
    13081 cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag gaactttgcg
    13141 aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc
    13201 agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag catttccaaa
    13261 ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt cactattttg
    13321 attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga
    13381 gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg
    13441 aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt
    13501 caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga caccgttagt
    13561 tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc
    13621 gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca gttgattgat
    13681 gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc ttacattaga
    13741 agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat
    13801 attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt ggatcgcatt
    13861 gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca aggaagaaat
    13921 gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc
    13981 aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt cgactttgtc
    14041 tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta attcagcttt
    14101 taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta ctacgtgatt
    14161 attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc ttccttcact
    14221 ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc cgaatagtcc
    14281 gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg
    14341 aactttatct accgctttgg tgatatcaga atctacattg actttgacat tttccacaac
    14401 tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt tgccagtgcc
    14461 catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc
    14521 cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg gtgtcacatt
    14581 ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt
    14641 atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact ttaggaaaaa
    14701 tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt gtaaaactac
    14761 gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat
    14821 aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaataacact
    14881 gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa
    14941 aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcatga
    15001 agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat taaaagaaaa
    15061 atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg caaaaatcga
    15121 attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca
    15181 agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag aatacgtcta
    15241 tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac aaatcgacca
    15301 attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt taggactcag
    15361 cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga cgtatccaca
    15421 tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga tcaatgcggg
    15481 ggctccatac cacaaataat gatatttttt aagagaaaaa attatcccta ttttaaataa
    15541 tagcaatagg tagtggtagg gagaaattta taaataactt ttttatttgc tttggtagcc
    15601 aagtaagttg caaaagaaaa atagatactg ttaaaaagat aatgtagaca tagaaacaaa
    15661 tacgtaaaat tttagttaga ataatggcaa ggagaaaaaa catgatagat gaaaaaaagt
    15721 gtttaaaaaa tctattagag gatatggatg atattgaaga aaactatcta aatggactgg
    15781 gaaaacaaat atatttcaga tacttaagct atctaaaaat gaaataagac atagtaattt
    15841 tttagctttt ttgtttgctc caaatgaaac ccacaattta tctgatgagt tttttaaaat
    15901 gtttttgaaa aggtatatag attctaatga tgataccaaa gctgctatca attattttga
    15961 tgctttactt aatagctatg aag
//
LOCUS       DAJSBC010000059        15926 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSBC010000059 DAJSBC010000000
VERSION     DAJSBC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.059"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            83..1081
                     /locus_tag="ONT65_002037"
     CDS             83..1081
                     /locus_tag="ONT65_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2329642.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="ONT65_002038"
     CDS             1105..1389
                     /locus_tag="ONT65_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329643.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="ONT65_002039"
     CDS             1470..1868
                     /locus_tag="ONT65_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2329644.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="ONT65_002040"
     CDS             1878..4421
                     /locus_tag="ONT65_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2329645.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="ONT65_002041"
     CDS             4443..6785
                     /locus_tag="ONT65_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329646.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="ONT65_002042"
     CDS             6801..8975
                     /locus_tag="ONT65_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCR2329647.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="ONT65_002043"
     CDS             8965..9387
                     /locus_tag="ONT65_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2329648.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10330..11241
                     /locus_tag="ONT65_002044"
     CDS             10330..11241
                     /locus_tag="ONT65_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR2329649.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11728..11994
                     /locus_tag="ONT65_002045"
     CDS             11728..11994
                     /locus_tag="ONT65_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329650.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12066..12407
                     /locus_tag="ONT65_002046"
     CDS             12066..12407
                     /locus_tag="ONT65_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329651.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12462..12881
                     /gene="ssb"
                     /locus_tag="ONT65_002047"
     CDS             12462..12881
                     /gene="ssb"
                     /locus_tag="ONT65_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2329652.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12958..13254
                     /locus_tag="ONT65_002048"
     CDS             12958..13254
                     /locus_tag="ONT65_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329653.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13269..13700
                     /locus_tag="ONT65_002049"
     CDS             13269..13700
                     /locus_tag="ONT65_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2329654.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13882..14187
                     /locus_tag="ONT65_002050"
     CDS             13882..14187
                     /locus_tag="ONT65_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329655.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14591..15079
                     /locus_tag="ONT65_002051"
     CDS             14591..15079
                     /locus_tag="ONT65_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2329656.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            15066..>15926
                     /locus_tag="ONT65_002052"
     CDS             15066..>15926
                     /locus_tag="ONT65_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329657.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQFRMFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFL
                     IFSLIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENIS
                     ILKFDSRFKELFNVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNF
                     VIVFMYANMVP"
BASE COUNT         5651 a         2375 c         3297 g         4603 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa acaagtgtag
    10201 aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa agcagttgca
    10261 gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt aaattgaagg
    10321 tgatgaaata tgaagtattt attgcatggc tattttattg attattctac tcgcaatagt
    10381 agtactttag cgattctatg tttgcttcat gaattgagag tggttactag aaaacagcta
    10441 tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa tgctttagtg
    10501 agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat gtattattat
    10561 ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa agttcctgaa
    10621 tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat ggcattggca
    10681 aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta tgaaacgaag
    10741 gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat ttttcgtttt
    10801 cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca
    10861 cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg aacacgtcct
    10921 gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact tcatgcgttt
    10981 aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga attgtttgag
    11041 cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt attagagaat
    11101 gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt
    11161 caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag
    11221 tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg tgccagtttg
    11281 ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt
    11341 tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt
    11401 cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg
    11461 cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc gctatacgaa
    11521 aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa aatcaaacaa
    11581 gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc cccaaacccc
    11641 cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca
    11701 ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact gaaagaacag
    11761 gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga aggagatttt
    11821 gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt agacccaatg
    11881 aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc gcaagatttc
    11941 actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt ataaaagtca
    12001 gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa agaaggaaaa
    12061 agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt agttggggct
    12121 acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt
    12181 ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga aaagttgttc
    12241 caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt attgtaaaaa
    12301 aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt tgggacacgt
    12361 ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg gatagtttcc
    12421 cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat tcagtgaatt
    12481 tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc aatgcgacag
    12541 gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag
    12601 attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa tatactggaa
    12661 aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag aatcagcaag
    12721 gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta gagagtaaag
    12781 aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct tcaattccag
    12841 gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat aattttgttc
    12901 tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg attttttatg
    12961 aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt tgattataca
    13021 gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta
    13081 gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga ttatgtcgga
    13141 gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac aggagaagaa
    13201 cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga ttaaagagga
    13261 gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg attgattttg
    13321 ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa ataaaacaag
    13381 caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata gtgaatcaaa
    13441 ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg caattttttt
    13501 cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat aaaagcagga
    13561 tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa caaaagaaaa
    13621 tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa
    13681 gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta atcacaacta
    13741 acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg tctgatactc
    13801 ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag gcggcagaac
    13861 aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa attactcagg
    13921 gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat aaccgactta
    13981 tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt
    14041 catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa aacatgtttg
    14101 attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat agagcaacta
    14161 gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa atcctctaca
    14221 gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa tttttaccat
    14281 aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa aaaatggaag
    14341 cggttactta ttcttttgaa tctaagataa aaattcaaaa gaataacata acccaaatat
    14401 gattatactt gagattctaa atatttatcg aaaaggaatt tgttttaagg aaaaatgatt
    14461 ctatggacgg ttattgattt atatttacca aatggctaat aaaatagtat ttcttatatt
    14521 gtatagtttt tgtttttatg tgaaaacatg agcaatagaa ttgtactagt aggataagga
    14581 tggatggaaa atgaaaaaaa ctatttttgt tttagcgatt tttatatgtt ttgggctact
    14641 tttggtaggc tacttgttgt ttaattctaa tgaaatgaga actatttcta cgaatgactt
    14701 gttaaaaaaa agtgaattaa aggataaaaa aaaagaatct cttgccttta taatgtttac
    14761 tcaagaagag tgtgagtatt gtatagcaat aaatccaatc atagatagtg taaataagga
    14821 aactttgaat aagataaaga aatatgatat atctgtagat tttcaatcta aacaaaagtg
    14881 taaaattaag tttgttccga ctattgttgc atatagtaag aataaagaag tggggagaat
    14941 tgacaatgaa ttagataatt tgtatcgaaa aaagcaagat ggttcaattg taatagacaa
    15001 gaaaaaaaca aagacattgc ttatagattg gatgaaaaaa atggataaac aaggagaata
    15061 tttgtatgga gaagattaaa cagacatttc cgttaattaa taaatattgg aataagaaaa
    15121 aaatttttat attttttatt acgtttctgc tgtcatcttt attttctcta attttcccgt
    15181 atctatttag taatgttatt gatttatatt tagagaatga gttatcacag aatattgtgc
    15241 gaatagtaac gattcttata tttcagttat ttttagtctt aattaaagaa gtgtatggat
    15301 atataaaaac tatttatgca tttgaagttg atttatcaat gaaaattgat tttcttaagg
    15361 ttttacaaaa aaaaaatctt tttttttcgc aaacgaagca ttcaggagaa attcaattta
    15421 ggatgttttc ggatatttct actatcgtaa atactatctt tattttttct gttgaattac
    15481 ctgtgatagg cgttttagtt cttgtagcgg tatttattat aagtaaagtg aatcttattt
    15541 ttctgatttt ttcactaata atgatgggag ctatggtgtt acagtataat ttatttcaat
    15601 taccgcttaa gaaaacaatc gagaatacaa aaagtgttaa tcaaagtatt acaggagaat
    15661 tgaatgcaca ttttgatcaa ttagaaatta taagcgcata taattttgag aatatctcta
    15721 ttttaaaatt cgattctaga tttaaagaat tatttaatgt tttcaaaaaa caaaataaat
    15781 tactaacact gggaagctgt ttggcacagg ttattttcca attatggatt ggaggaatat
    15841 ttcttattga tgttatttta ttagctaaag gaacaataag tattactaat tttgtaatag
    15901 tatttatgta cgctaacatg gtccct
//
LOCUS       DAJSBC010000060        15230 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSBC010000060 DAJSBC010000000
VERSION     DAJSBC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.060"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            278..787
                     /locus_tag="ONT65_002053"
     CDS             278..787
                     /locus_tag="ONT65_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2329658.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ONT65_002054"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ONT65_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2329659.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ONT65_002055"
     CDS             2322..3302
                     /locus_tag="ONT65_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2329660.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ONT65_002056"
     CDS             3424..4842
                     /locus_tag="ONT65_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR2329661.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ONT65_002057"
     CDS             4913..6382
                     /locus_tag="ONT65_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2329662.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ONT65_002058"
     CDS             6392..6859
                     /locus_tag="ONT65_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2329663.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="ONT65_002059"
     CDS             6895..7767
                     /locus_tag="ONT65_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2329664.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ONT65_002060"
     CDS             7848..10382
                     /locus_tag="ONT65_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2329665.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ONT65_002061"
     CDS             10538..11311
                     /locus_tag="ONT65_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2329666.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ONT65_002062"
     CDS             11304..12095
                     /locus_tag="ONT65_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2329667.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ONT65_002063"
     CDS             12105..12596
                     /locus_tag="ONT65_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2329668.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ONT65_002064"
     CDS             12614..13024
                     /locus_tag="ONT65_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2329669.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ONT65_002065"
     CDS             13028..14428
                     /locus_tag="ONT65_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR2329670.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ONT65_002066"
     CDS             14451..14825
                     /locus_tag="ONT65_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2329671.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAJSBC010000061        14939 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSBC010000061 DAJSBC010000000
VERSION     DAJSBC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.061"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..562
                     /locus_tag="ONT65_002067"
     CDS             <1..562
                     /locus_tag="ONT65_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2329672.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ONT65_002068"
     CDS             762..1076
                     /locus_tag="ONT65_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2329673.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ONT65_002069"
     CDS             1097..1474
                     /locus_tag="ONT65_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2329674.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ONT65_002070"
     CDS             1484..2257
                     /locus_tag="ONT65_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329675.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ONT65_002071"
     CDS             2279..3682
                     /locus_tag="ONT65_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2329676.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="ONT65_002072"
     CDS             3864..5048
                     /locus_tag="ONT65_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2329677.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ONT65_002073"
     CDS             5045..5335
                     /locus_tag="ONT65_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329678.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ONT65_002074"
     CDS             5332..5553
                     /locus_tag="ONT65_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329679.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ONT65_002075"
     CDS             5595..6374
                     /locus_tag="ONT65_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCR2329680.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="ONT65_002076"
     CDS             6441..6935
                     /locus_tag="ONT65_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2329681.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="ONT65_002077"
     CDS             6991..7629
                     /locus_tag="ONT65_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329682.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ONT65_002078"
     CDS             7701..8096
                     /locus_tag="ONT65_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2329683.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ONT65_002079"
     CDS             8080..10533
                     /locus_tag="ONT65_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2329684.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ONT65_002080"
     CDS             10530..12557
                     /locus_tag="ONT65_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329685.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ONT65_002081"
     CDS             12554..13576
                     /locus_tag="ONT65_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2329686.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ONT65_002082"
     CDS             13593..14522
                     /locus_tag="ONT65_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2329687.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="ONT65_002083"
     CDS             14767..14883
                     /locus_tag="ONT65_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2329688.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         5072 a         2485 c         2954 g         4428 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DAJSBC010000062        14815 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSBC010000062 DAJSBC010000000
VERSION     DAJSBC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.062"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(296..1024)
                     /locus_tag="ONT65_002084"
     CDS             complement(296..1024)
                     /locus_tag="ONT65_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR2329689.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ONT65_002085"
     CDS             complement(1002..2576)
                     /locus_tag="ONT65_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2329690.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ONT65_002086"
     CDS             2805..3743
                     /locus_tag="ONT65_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2329691.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ONT65_002087"
     CDS             3755..4666
                     /locus_tag="ONT65_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2329692.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ONT65_002088"
     CDS             4690..6162
                     /locus_tag="ONT65_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2329693.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ONT65_002089"
     CDS             6213..6824
                     /locus_tag="ONT65_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2329694.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ONT65_002090"
     CDS             6854..8767
                     /locus_tag="ONT65_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCR2329695.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ONT65_002091"
     CDS             8895..11876
                     /locus_tag="ONT65_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCR2329696.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ONT65_002092"
     CDS             11873..13534
                     /locus_tag="ONT65_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCR2329697.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ONT65_002093"
     CDS             13574..14506
                     /locus_tag="ONT65_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR2329698.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAJSBC010000063        14737 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSBC010000063 DAJSBC010000000
VERSION     DAJSBC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.064"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            200..1096
                     /locus_tag="ONT65_002094"
     CDS             200..1096
                     /locus_tag="ONT65_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2329699.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1246..2187)
                     /locus_tag="ONT65_002095"
     CDS             complement(1246..2187)
                     /locus_tag="ONT65_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2329700.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2313..3434
                     /locus_tag="ONT65_002096"
     CDS             2313..3434
                     /locus_tag="ONT65_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2329701.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3692..5194
                     /gene="eat(A)"
                     /locus_tag="ONT65_002097"
     CDS             3692..5194
                     /gene="eat(A)"
                     /locus_tag="ONT65_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2329702.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5222..5788
                     /locus_tag="ONT65_002098"
     CDS             5222..5788
                     /locus_tag="ONT65_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2329703.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5999..7060
                     /locus_tag="ONT65_002099"
     CDS             5999..7060
                     /locus_tag="ONT65_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2329704.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7063..7959
                     /gene="murQ"
                     /locus_tag="ONT65_002100"
     CDS             7063..7959
                     /gene="murQ"
                     /locus_tag="ONT65_002100"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2329705.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7973..9955
                     /locus_tag="ONT65_002101"
     CDS             7973..9955
                     /locus_tag="ONT65_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2329706.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10020..10466)
                     /gene="nrdI"
                     /locus_tag="ONT65_002102"
     CDS             complement(10020..10466)
                     /gene="nrdI"
                     /locus_tag="ONT65_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2329707.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10677..12101
                     /locus_tag="ONT65_002103"
     CDS             10677..12101
                     /locus_tag="ONT65_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2329708.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12282..13055)
                     /locus_tag="ONT65_002104"
     CDS             complement(12282..13055)
                     /locus_tag="ONT65_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2329709.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13052..13957)
                     /locus_tag="ONT65_002105"
     CDS             complement(13052..13957)
                     /locus_tag="ONT65_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2329710.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14111..14431)
                     /locus_tag="ONT65_002106"
     CDS             complement(14111..14431)
                     /locus_tag="ONT65_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2329711.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4716 a         2623 c         3109 g         4289 t
ORIGIN      
        1 actaatgata acaaattttc agacaattac aactattcag aaaactttta ggcgaatttt
       61 aatagaagaa tgatttattt catcatattt tcatacaaat gttaaatttt catacaaata
      121 taggaaacgt tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag
      181 agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc
      241 actttatttt ttgccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca
      301 gtcggaattc attaaatcag ctagacaaca gttggcaaat tatcagctga tcgttttttt
      361 agattatgga tttgatatgg agatggctcg acgcgtgcgc ccatttacca atgcaaaaat
      421 tgtgttattt ttttggaatc acttcaaagc agaacacgag gcgatgctga agcaggcaca
      481 agatgaaccg gcaatcgatg agatttatca ttttgatttt ttggaagcaa aagaacttgg
      541 gctgaaacat aatagttcat tttattcgaa acatatgaat ttttcttcta gcgaaccaaa
      601 gacggatcta ttttttggtg caacgaataa tgggagaaaa gaacgagcag aaagctataa
      661 gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac atcgagggaa
      721 tgaacaacct ggatatctta cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg
      781 gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat ttgaatccat
      841 gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc atttttatcg
      901 tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg aatttctgca
      961 tacaccattt aagccaatcc tatcagagct attggaattt tatgatgcag aaaattgggc
     1021 aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg acctctcctc
     1081 tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa caaacaagca
     1141 caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg ggcttaaaaa
     1201 aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt ctgcgccaag
     1261 gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc
     1321 atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag tcaaacgatg
     1381 gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata
     1441 agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt cttttgctgc
     1501 gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc
     1561 tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc catttacaca
     1621 agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta aatttgcttc
     1681 ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag
     1741 ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca
     1801 catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg
     1861 acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga
     1921 acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc
     1981 gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc
     2041 gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc
     2101 gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc
     2161 tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt
     2221 ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag
     2281 tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca attgattcat
     2341 ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc
     2401 gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa
     2461 tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc
     2521 agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat
     2581 ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg
     2641 cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa
     2701 ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct
     2761 tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc
     2821 attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta
     2881 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
     2941 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
     3001 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
     3061 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
     3121 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
     3181 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
     3241 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
     3301 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
     3361 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
     3421 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
     3481 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
     3541 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
     3601 tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata
     3661 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
     3721 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
     3781 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     3841 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     3901 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     3961 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     4021 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     4081 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     4141 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     4201 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     4261 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     4321 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     4381 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     4441 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     4501 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     4561 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     4621 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     4681 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     4741 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     4801 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     4861 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     4921 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     4981 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     5041 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     5101 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     5161 atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg
     5221 tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca
     5281 cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac
     5341 tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc
     5401 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc
     5461 tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga
     5521 ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt
     5581 acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa
     5641 agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag
     5701 agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga
     5761 taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt
     5821 caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata
     5881 taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt
     5941 ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga gggataatat
     6001 gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg
     6061 ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga
     6121 tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact
     6181 agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga
     6241 tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa
     6301 tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac
     6361 gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg
     6421 gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa
     6481 tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt
     6541 gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct
     6601 tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg
     6661 tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct
     6721 ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg
     6781 gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc
     6841 tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa
     6901 gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa
     6961 agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc
     7021 tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta
     7081 gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt
     7141 tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa
     7201 gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga
     7261 cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa
     7321 tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa
     7381 gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat
     7441 ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt
     7501 acaccatatg tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg
     7561 atttcttgca ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat
     7621 gcaggtccag aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt
     7681 atcttgaata tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg
     7741 atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg
     7801 caagcgacag aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat
     7861 gttaagctgg ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa
     7921 ttagctgaag caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa
     7981 aaacgaactg actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg
     8041 caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga
     8101 tcttgtaaat gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga
     8161 acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga
     8221 tttggcaggc gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc
     8281 atcagcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa
     8341 atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt
     8401 gccaatgatt cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc
     8461 aaacttagtc acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat
     8521 gaacatcttg aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc
     8581 acaagttttt ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac
     8641 agggatgaat ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg
     8701 acaaggtggg atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa
     8761 actgcataaa gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat
     8821 tgccatcggt ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg
     8881 catggtcggt gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct
     8941 aggcacatta ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca
     9001 tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg
     9061 tgcaggacaa gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt
     9121 aacagaaatg atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat
     9181 ttacggagta acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat
     9241 cggcggggca gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg
     9301 agccgctttg atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt
     9361 ggcagcttat gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt
     9421 agaaaaagaa gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt
     9481 acctcaaact tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt
     9541 ttctttagat aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt
     9601 tgtagaacca gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt
     9661 cccaacgaaa cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat
     9721 gggaatcaac acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca
     9781 gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg
     9841 caaagaaaca tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt
     9901 ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga
     9961 tagaaacaat agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat
    10021 tatttattgt tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt
    10081 aattcataat ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga
    10141 caatactgat tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga
    10201 ttgtcttcat gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta
    10261 tctaaaccat ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct
    10321 ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct
    10381 tctgcatgca ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct
    10441 gaaatcgata tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc
    10501 atctttttta agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata
    10561 atgagaaaaa agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat
    10621 aaaaaaggtt tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg
    10681 atacagagca acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt
    10741 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc
    10801 cagctatttt cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca
    10861 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
    10921 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
    10981 gcaacgtagc ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg
    11041 aaggtgggca aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat
    11101 ggctagtgaa tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta
    11161 aattagtgcc attattttta tttattgtag tagctatcag cacgttttcg atccaacaat
    11221 tcattgataa cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt
    11281 ccagtcaagt attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag
    11341 cctctgtcgt aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag
    11401 ggatattcgg cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga
    11461 cgatgaatca gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga
    11521 tggttggtaa atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg
    11581 tttggctctc atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac
    11641 ttttgcctaa agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat
    11701 tgacgacgtt atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata
    11761 actttgctta cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt
    11821 accaaatgaa actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag
    11881 caagtgcctt tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc
    11941 ttgttctttt cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc
    12001 ctgtacaaca tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga
    12061 ttgcaatggt tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa
    12121 agagaggttg tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa
    12181 atgttgtcac agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata
    12241 aggctgaacg tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta
    12301 aatacgagat atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt
    12361 tgattagtag aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc
    12421 agattcaaca tactgatcgg attccatttg atccactccc atttttggat gagtgcaata
    12481 aggataccag atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgcg
    12541 ccggaactat tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg
    12601 atataactac ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc
    12661 ttctccgtga aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata
    12721 gctaaaaggt ataaataaac agaataaata actaatgtga tccatttgct tactaatatt
    12781 tctcctcgag aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc
    12841 gaaatgatcg tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa
    12901 agctgagcag tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct
    12961 gttcctatga gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc
    13021 aatttaaata attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat
    13081 ccagaatttc caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat
    13141 ggatattttg tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta
    13201 aaagcttgat tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag
    13261 cagctagatc atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag
    13321 ccattgtaca ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt
    13381 tttcaagctc gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt
    13441 cggtaatgat ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat
    13501 ctaaaataac gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca
    13561 ttcccaacga gtaggatttc gcttttttgt gaatgtactt ttccatcttg agctgattca
    13621 cgatagtgtc gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc
    13681 ctgttaagaa tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt
    13741 tatgactaga gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga
    13801 tcagtcctaa aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc
    13861 caacgatttt gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa
    13921 agttcttgga taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag
    13981 atagtacaag catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta
    14041 ttttttcata aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta
    14101 ttccaagtgt ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc
    14161 ccacaaagaa ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga
    14221 ccgaaccaac tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt
    14281 tgattgcctt tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat
    14341 aattgatttt agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa
    14401 ttcctgctat gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa
    14461 tacacaaaaa gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc
    14521 ctacccggtg gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg
    14581 caatctcata ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa
    14641 gaagatgaat gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa
    14701 aacagtaact agaccttatt tctatatatt atcgggc
//
LOCUS       DAJSBC010000064        14416 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSBC010000064 DAJSBC010000000
VERSION     DAJSBC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.065"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(535..1038)
                     /locus_tag="ONT65_002107"
     CDS             complement(535..1038)
                     /locus_tag="ONT65_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2329712.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1397..2422
                     /locus_tag="ONT65_002108"
     CDS             1397..2422
                     /locus_tag="ONT65_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2329713.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2745..3623
                     /locus_tag="ONT65_002109"
     CDS             2745..3623
                     /locus_tag="ONT65_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR2329714.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4099..4215
                     /locus_tag="ONT65_002110"
     CDS             4099..4215
                     /locus_tag="ONT65_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2329715.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5143..5481
                     /locus_tag="ONT65_002111"
     CDS             5143..5481
                     /locus_tag="ONT65_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329716.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5575..5805
                     /locus_tag="ONT65_002112"
     CDS             5575..5805
                     /locus_tag="ONT65_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329717.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6205..6576
                     /locus_tag="ONT65_002113"
     CDS             6205..6576
                     /locus_tag="ONT65_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2329718.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6850..7542)
                     /locus_tag="ONT65_002114"
     CDS             complement(6850..7542)
                     /locus_tag="ONT65_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2329719.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7545..8330)
                     /locus_tag="ONT65_002115"
     CDS             complement(7545..8330)
                     /locus_tag="ONT65_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2329720.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8567..9967
                     /gene="lacG"
                     /locus_tag="ONT65_002116"
     CDS             8567..9967
                     /gene="lacG"
                     /locus_tag="ONT65_002116"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR2329721.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9992..10309
                     /locus_tag="ONT65_002117"
     CDS             9992..10309
                     /locus_tag="ONT65_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2329722.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10322..11995
                     /locus_tag="ONT65_002118"
     CDS             10322..11995
                     /locus_tag="ONT65_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR2329723.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12009..13640
                     /locus_tag="ONT65_002119"
     CDS             12009..13640
                     /locus_tag="ONT65_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR2329724.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         4594 a         2336 c         2927 g         4559 t
ORIGIN      
        1 ggaataaaaa aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac
       61 gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc
      121 ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag
      181 ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct
      241 ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga
      301 ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct
      361 ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa
      421 taatatagta gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac
      481 cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg
      541 aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta
      601 tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg
      661 ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc
      721 tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat
      781 ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg
      841 aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa
      901 agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt
      961 aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa
     1021 ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct
     1081 gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga
     1141 agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga
     1201 aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc
     1261 tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact
     1321 aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt tccaattact
     1381 aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc
     1441 ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa
     1501 tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca
     1561 aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt
     1621 tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc
     1681 tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg
     1741 cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt
     1801 accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat
     1861 tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag
     1921 atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct
     1981 ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg
     2041 tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct
     2101 gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca
     2161 gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag
     2221 aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat
     2281 tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag
     2341 tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt
     2401 tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga
     2461 tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa
     2521 ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa
     2581 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
     2641 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
     2701 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
     2761 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
     2821 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
     2881 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
     2941 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
     3001 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
     3061 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
     3121 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
     3181 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
     3241 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
     3301 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
     3361 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
     3421 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
     3481 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
     3541 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
     3601 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
     3661 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
     3721 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
     3781 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
     3841 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
     3901 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
     3961 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
     4021 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
     4081 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
     4141 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
     4201 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
     4261 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
     4321 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
     4381 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
     4441 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
     4501 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
     4561 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
     4621 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
     4681 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
     4741 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
     4801 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
     4861 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
     4921 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
     4981 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
     5041 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
     5101 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
     5161 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
     5221 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
     5281 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
     5341 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
     5401 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
     5461 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
     5521 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
     5581 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
     5641 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
     5701 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
     5761 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
     5821 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
     5881 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
     5941 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
     6001 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
     6061 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
     6121 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
     6181 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
     6241 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
     6301 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
     6361 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
     6421 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
     6481 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
     6541 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
     6601 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
     6661 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
     6721 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
     6781 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
     6841 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
     6901 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
     6961 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
     7021 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
     7081 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
     7141 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
     7201 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
     7261 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
     7321 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
     7381 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
     7441 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
     7501 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
     7561 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
     7621 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
     7681 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
     7741 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
     7801 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
     7861 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
     7921 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
     7981 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
     8041 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
     8101 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
     8161 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
     8221 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
     8281 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
     8341 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
     8401 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
     8461 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
     8521 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
     8581 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
     8641 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
     8701 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
     8761 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
     8821 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
     8881 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
     8941 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
     9001 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
     9061 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
     9121 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
     9181 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
     9241 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
     9301 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
     9361 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
     9421 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
     9481 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
     9541 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
     9601 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
     9661 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
     9721 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
     9781 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
     9841 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
     9901 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
     9961 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    10021 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    10081 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    10141 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    10201 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    10261 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    10321 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    10381 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    10441 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    10501 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    10561 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    10621 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    10681 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    10741 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    10801 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    10861 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    10921 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    10981 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    11041 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    11101 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    11161 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    11221 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    11281 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    11341 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    11401 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    11461 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    11521 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    11581 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    11641 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    11701 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    11761 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    11821 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    11881 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    11941 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    12001 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    12061 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    12121 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    12181 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    12241 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    12301 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    12361 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    12421 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    12481 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    12541 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    12601 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    12661 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    12721 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    12781 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    12841 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    12901 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    12961 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    13021 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    13081 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    13141 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    13201 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    13261 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    13321 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    13381 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    13441 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    13501 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    13561 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    13621 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    13681 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    13741 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    13801 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    13861 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    13921 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    13981 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    14041 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    14101 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    14161 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    14221 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    14281 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    14341 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    14401 acccaattgt tataat
//
LOCUS       DAJSBC010000065        14286 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSBC010000065 DAJSBC010000000
VERSION     DAJSBC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.066"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(221..1423)
                     /locus_tag="ONT65_002120"
     CDS             complement(221..1423)
                     /locus_tag="ONT65_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2329725.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ONT65_002121"
     CDS             complement(1426..1620)
                     /locus_tag="ONT65_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2329726.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ONT65_002122"
     CDS             complement(1667..2515)
                     /locus_tag="ONT65_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCR2329727.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ONT65_002123"
     CDS             complement(2720..3070)
                     /locus_tag="ONT65_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329728.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="ONT65_002124"
     CDS             3665..4111
                     /locus_tag="ONT65_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2329729.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ONT65_002125"
     CDS             4735..5727
                     /locus_tag="ONT65_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2329730.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ONT65_002126"
     CDS             5743..6627
                     /locus_tag="ONT65_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2329731.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ONT65_002127"
     CDS             6676..8283
                     /locus_tag="ONT65_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2329732.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ONT65_002128"
     CDS             8284..9768
                     /locus_tag="ONT65_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR2329733.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ONT65_002129"
     CDS             9945..10937
                     /locus_tag="ONT65_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2329734.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ONT65_002130"
     CDS             10989..11858
                     /locus_tag="ONT65_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2329735.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ONT65_002131"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ONT65_002131"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2329736.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13898..>14286)
                     /locus_tag="ONT65_002132"
     CDS             complement(13898..>14286)
                     /locus_tag="ONT65_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2329737.1"
                     /translation="RHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKR
                     GTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIK
                     RIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         4366 a         2424 c         2951 g         4545 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacctt
    13921 gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt
    13981 taatcagctt gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg gacaccttaa
    14041 aggcatttga aatacccgat tggtagcgat ggaagatcgt gaatttcttt ttaaacgact
    14101 gaggtaacgt agatgaaatt gttttaaggg tttcttgaaa gccttccgta cctcgtttaa
    14161 cgaaataata gcgtaataat tgacaaaaat gatacgcagt tcgcaattct tcgttgtagc
    14221 ttaacagctc atcaacgatt tctgtttgac taattggtcg tttaaacagt cgatgataat
    14281 gccgtt
//
LOCUS       DAJSBC010000066        14107 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSBC010000066 DAJSBC010000000
VERSION     DAJSBC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.067"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            66..767
                     /locus_tag="ONT65_002133"
     CDS             66..767
                     /locus_tag="ONT65_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR2329738.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ONT65_002134"
     CDS             764..1885
                     /locus_tag="ONT65_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR2329739.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ONT65_002135"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ONT65_002135"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR2329740.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ONT65_002136"
     CDS             3264..4172
                     /locus_tag="ONT65_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR2329741.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ONT65_002137"
     CDS             4207..4479
                     /locus_tag="ONT65_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR2329742.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ONT65_002138"
     CDS             4490..5536
                     /locus_tag="ONT65_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR2329743.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ONT65_002139"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ONT65_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR2329744.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ONT65_002140"
     CDS             complement(8547..9233)
                     /locus_tag="ONT65_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329745.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ONT65_002141"
     CDS             complement(9211..9705)
                     /locus_tag="ONT65_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2329746.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ONT65_002142"
     CDS             complement(9725..10945)
                     /locus_tag="ONT65_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2329747.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ONT65_002143"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ONT65_002143"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR2329748.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14107)
                     /locus_tag="ONT65_002144"
     CDS             complement(13021..>14107)
                     /locus_tag="ONT65_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2329749.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DAJSBC010000067        13852 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSBC010000067 DAJSBC010000000
VERSION     DAJSBC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.068"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..902)
                     /locus_tag="ONT65_002145"
     CDS             complement(<1..902)
                     /locus_tag="ONT65_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR2329750.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ONT65_002146"
     CDS             1185..2153
                     /locus_tag="ONT65_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2329751.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ONT65_002147"
     CDS             2266..2544
                     /locus_tag="ONT65_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2329752.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ONT65_002148"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ONT65_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR2329753.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ONT65_002149"
     CDS             3001..3756
                     /locus_tag="ONT65_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2329754.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ONT65_002150"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ONT65_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR2329755.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ONT65_002151"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ONT65_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR2329756.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ONT65_002152"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ONT65_002152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR2329757.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ONT65_002153"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ONT65_002153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR2329758.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ONT65_002154"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ONT65_002154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR2329759.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ONT65_002155"
     CDS             8493..9035
                     /locus_tag="ONT65_002155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR2329760.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="ONT65_002156"
     CDS             9058..10614
                     /locus_tag="ONT65_002156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR2329761.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ONT65_002157"
     CDS             10628..11530
                     /locus_tag="ONT65_002157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR2329762.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ONT65_002158"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ONT65_002158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR2329763.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ONT65_002159"
     CDS             12973..13395
                     /locus_tag="ONT65_002159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR2329764.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DAJSBC010000068        13835 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSBC010000068 DAJSBC010000000
VERSION     DAJSBC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.069"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            206..544
                     /locus_tag="ONT65_002160"
     CDS             206..544
                     /locus_tag="ONT65_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329765.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="ONT65_002161"
     CDS             complement(749..1324)
                     /locus_tag="ONT65_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR2329766.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2056..2637)
                     /locus_tag="ONT65_002162"
     CDS             complement(2056..2637)
                     /locus_tag="ONT65_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2329767.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2820..3446)
                     /locus_tag="ONT65_002163"
     CDS             complement(2820..3446)
                     /locus_tag="ONT65_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2329768.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3468..4799)
                     /locus_tag="ONT65_002164"
     CDS             complement(3468..4799)
                     /locus_tag="ONT65_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR2329769.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4954..5355)
                     /locus_tag="ONT65_002165"
     CDS             complement(4954..5355)
                     /locus_tag="ONT65_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2329770.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5441..5647)
                     /locus_tag="ONT65_002166"
     CDS             complement(5441..5647)
                     /locus_tag="ONT65_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329771.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5742..7244)
                     /locus_tag="ONT65_002167"
     CDS             complement(5742..7244)
                     /locus_tag="ONT65_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR2329772.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7262..7717)
                     /gene="argR"
                     /locus_tag="ONT65_002168"
     CDS             complement(7262..7717)
                     /gene="argR"
                     /locus_tag="ONT65_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2329773.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7815..8432)
                     /locus_tag="ONT65_002169"
     CDS             complement(7815..8432)
                     /locus_tag="ONT65_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR2329774.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8653..10026
                     /locus_tag="ONT65_002170"
     CDS             8653..10026
                     /locus_tag="ONT65_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329775.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10101..11213
                     /locus_tag="ONT65_002171"
     CDS             10101..11213
                     /locus_tag="ONT65_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR2329776.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11285..12922)
                     /locus_tag="ONT65_002172"
     CDS             complement(11285..12922)
                     /locus_tag="ONT65_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR2329777.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13090..13698)
                     /locus_tag="ONT65_002173"
     CDS             complement(13090..13698)
                     /locus_tag="ONT65_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2329778.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3749 a         2899 c         2472 g         4715 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     1681 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     1741 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1801 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     1861 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     1921 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     1981 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2041 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2101 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2161 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2221 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     2281 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     2341 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     2401 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     2461 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     2521 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     2581 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     2641 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     2701 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     2761 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     2821 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     2881 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     2941 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3001 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3061 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3121 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3181 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3241 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     3301 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     3361 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     3421 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     3481 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     3541 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     3601 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     3661 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     3721 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     3781 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     3841 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     3901 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     3961 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4021 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4081 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4141 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4201 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4261 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     4321 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     4381 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     4441 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     4501 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     4561 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     4621 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     4681 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     4741 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     4801 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     4861 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     4921 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     4981 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5041 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5101 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5161 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5221 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     5281 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     5341 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     5401 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     5461 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     5521 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     5581 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     5641 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     5701 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     5761 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     5821 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     5881 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     5941 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6001 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6061 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6121 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6181 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6241 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     6301 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     6361 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     6421 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     6481 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     6541 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     6601 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     6661 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     6721 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     6781 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     6841 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     6901 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     6961 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7021 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7081 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7141 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7201 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7261 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     7321 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     7381 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     7441 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     7501 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     7561 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     7621 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     7681 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     7741 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     7801 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     7861 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     7921 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     7981 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8041 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8101 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8161 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8221 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     8281 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     8341 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     8401 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     8461 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     8521 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     8581 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     8641 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     8701 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     8761 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     8821 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     8881 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     8941 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9001 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9061 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9121 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9181 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9241 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
     9301 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
     9361 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
     9421 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
     9481 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
     9541 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
     9601 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
     9661 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
     9721 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
     9781 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
     9841 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
     9901 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
     9961 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10021 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10081 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10141 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10201 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10261 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    10321 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    10381 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    10441 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    10501 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    10561 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    10621 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    10681 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    10741 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    10801 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    10861 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    10921 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    10981 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11041 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11101 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11161 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11221 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    11281 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    11341 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    11401 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    11461 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    11521 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    11581 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    11641 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    11701 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    11761 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    11821 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    11881 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    11941 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12001 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12061 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12121 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12181 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12241 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    12301 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    12361 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    12421 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    12481 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    12541 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    12601 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    12661 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    12721 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    12781 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    12841 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    12901 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    12961 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13021 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13081 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13141 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13201 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13261 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    13321 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    13381 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    13441 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    13501 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    13561 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    13621 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    13681 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    13741 attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt taatgacaaa
    13801 ataacgaatt atttaatatt tgtaacattt aaaca
//
LOCUS       DAJSBC010000069        13731 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSBC010000069 DAJSBC010000000
VERSION     DAJSBC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.070"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..1452
                     /locus_tag="ONT65_002174"
     CDS             1..1452
                     /locus_tag="ONT65_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2329779.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ONT65_002175"
     CDS             1801..2433
                     /locus_tag="ONT65_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR2329780.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ONT65_002176"
     CDS             3021..4460
                     /locus_tag="ONT65_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2329781.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ONT65_002177"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ONT65_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="ONT65_002178"
     CDS             5222..5437
                     /locus_tag="ONT65_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329782.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ONT65_002179"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ONT65_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ONT65_002180"
     CDS             complement(6097..7008)
                     /locus_tag="ONT65_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2329783.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ONT65_002181"
     CDS             complement(7027..8031)
                     /locus_tag="ONT65_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2329784.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ONT65_002182"
     CDS             complement(8028..10151)
                     /locus_tag="ONT65_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2329785.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ONT65_002183"
     CDS             complement(10156..12603)
                     /locus_tag="ONT65_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2329786.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ONT65_002184"
     CDS             complement(12590..12979)
                     /locus_tag="ONT65_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2329787.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ONT65_002185"
     CDS             complement(13039..13542)
                     /locus_tag="ONT65_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2329788.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13716)
                     /locus_tag="ONT65_002186"
     CDS             complement(13555..13716)
                     /locus_tag="ONT65_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329789.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DAJSBC010000070        13398 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSBC010000070 DAJSBC010000000
VERSION     DAJSBC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.071"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(14..706)
                     /locus_tag="ONT65_002187"
     CDS             complement(14..706)
                     /locus_tag="ONT65_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2329790.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="ONT65_002188"
     CDS             complement(1143..1712)
                     /locus_tag="ONT65_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2329791.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ONT65_002189"
     CDS             complement(1786..2109)
                     /locus_tag="ONT65_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329792.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ONT65_002190"
     CDS             complement(2263..3075)
                     /locus_tag="ONT65_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329793.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="ONT65_002191"
     CDS             3429..4277
                     /locus_tag="ONT65_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR2329794.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="ONT65_002192"
     CDS             4422..5363
                     /locus_tag="ONT65_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR2329795.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ONT65_002193"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ONT65_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT65_002194"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT65_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR2329796.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ONT65_002195"
     CDS             complement(9827..12013)
                     /locus_tag="ONT65_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR2329797.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT65_002196"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT65_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR2329798.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ONT65_002197"
     CDS             complement(12235..12675)
                     /locus_tag="ONT65_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR2329799.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ONT65_002198"
     CDS             complement(12749..13288)
                     /locus_tag="ONT65_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2329800.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAJSBC010000071        12918 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSBC010000071 DAJSBC010000000
VERSION     DAJSBC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.072"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            355..912
                     /locus_tag="ONT65_002199"
                     /pseudo
     CDS             355..912
                     /locus_tag="ONT65_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            970..3009
                     /locus_tag="ONT65_002200"
     CDS             970..3009
                     /locus_tag="ONT65_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR2329801.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKIRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3118..4269)
                     /locus_tag="ONT65_002201"
     CDS             complement(3118..4269)
                     /locus_tag="ONT65_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR2329802.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4423..4953)
                     /locus_tag="ONT65_002202"
     CDS             complement(4423..4953)
                     /locus_tag="ONT65_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2329803.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5052..5402)
                     /locus_tag="ONT65_002203"
     CDS             complement(5052..5402)
                     /locus_tag="ONT65_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR2329804.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5559..7088)
                     /gene="ahpF"
                     /locus_tag="ONT65_002204"
     CDS             complement(5559..7088)
                     /gene="ahpF"
                     /locus_tag="ONT65_002204"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR2329805.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7098..7661)
                     /gene="ahpC"
                     /locus_tag="ONT65_002205"
     CDS             complement(7098..7661)
                     /gene="ahpC"
                     /locus_tag="ONT65_002205"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR2329806.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7805..8467)
                     /locus_tag="ONT65_002206"
     CDS             complement(7805..8467)
                     /locus_tag="ONT65_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2329807.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8651..10297
                     /locus_tag="ONT65_002207"
     CDS             8651..10297
                     /locus_tag="ONT65_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2329808.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10288..11370
                     /gene="uxuA"
                     /locus_tag="ONT65_002208"
     CDS             10288..11370
                     /gene="uxuA"
                     /locus_tag="ONT65_002208"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2329809.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11478..12918)
                     /locus_tag="ONT65_002209"
                     /pseudo
     CDS             complement(11478..12918)
                     /locus_tag="ONT65_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4317 a         2373 c         2411 g         3817 t
ORIGIN      
        1 attaggaaaa gtctatcatg aactcgtaaa tagaatatag taataacctt gttaggtgag
       61 gctcctatac aaacataggc tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca
      121 gaaactgttg gattaaggct ttttccaata gctaagaatg ttttctttac gttgtatagt
      181 gctaaaactc ggacgatgag atgatgctag gtcagaattc ttgttcgaaa aggtttctga
      241 ccttttcttt atatttttgt gactgactta tttgatgaga caaactcgct cctcacctga
      301 caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg tggaggtcgc
      361 tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct
      421 atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct
      481 tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa
      541 gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt
      601 cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac
      661 aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac
      721 aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag
      781 cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt
      841 agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa
      901 gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag
      961 gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc
     1021 ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa
     1081 gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac
     1141 aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca
     1201 ttatctgaaa aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa
     1261 ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca
     1321 tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca
     1381 ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa
     1441 atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata
     1501 gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca
     1561 agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc
     1621 gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac
     1681 tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt
     1741 caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac
     1801 gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa
     1861 atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg
     1921 aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt
     1981 caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaat agcttttgac
     2041 agtctaggag gaaaagctgc atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt
     2101 gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa
     2161 gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt
     2221 tatgctcctg gatctacgtt taaaatgatt acagcagcaa tcggtctcga caacggcact
     2281 atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg
     2341 ggatctttta aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct
     2401 ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa
     2461 attcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg
     2521 aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact
     2581 ggatatggac agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt
     2641 tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat
     2701 aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt
     2761 gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg
     2821 aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc
     2881 ttgtttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa
     2941 gaagatgatg attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat
     3001 tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc
     3061 cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca
     3121 cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa
     3181 taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc
     3241 aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa
     3301 tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt
     3361 caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa
     3421 atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc
     3481 ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa
     3541 aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca
     3601 aataactagc gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag
     3661 taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc
     3721 aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct
     3781 gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga
     3841 tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga
     3901 agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt
     3961 gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc
     4021 aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa
     4081 taagatcaca ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa
     4141 tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa
     4201 tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat
     4261 aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat
     4321 tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg
     4381 attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt
     4441 tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa
     4501 tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat
     4561 caacaacaga atactactat aaacactcca acttttagct tttgtattca tgctgaagaa
     4621 caactggaac aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac
     4681 gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa
     4741 cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa
     4801 caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga
     4861 agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta
     4921 tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt
     4981 aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat
     5041 ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg
     5101 atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt
     5161 acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca
     5221 aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa
     5281 cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct
     5341 tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc
     5401 ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga
     5461 gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc
     5521 cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg
     5581 aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga
     5641 ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg
     5701 gtcactattt catttcttgg attcatttcg atcgttctat ctagccaatc cgtattaggg
     5761 agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg
     5821 cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca
     5881 tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct
     5941 tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt
     6001 gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa
     6061 gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt
     6121 acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat
     6181 ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt
     6241 tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat
     6301 tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact
     6361 tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata
     6421 gctgcactac tagctgctgg tccaccgcca atcactaata catcataagg ttctttttca
     6481 aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg
     6541 cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta
     6601 tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt
     6661 aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta
     6721 acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca
     6781 ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca
     6841 tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa
     6901 aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct
     6961 atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa
     7021 aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg
     7081 tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg
     7141 ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca
     7201 gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc
     7261 atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc
     7321 tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg
     7381 tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta
     7441 tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt
     7501 aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata
     7561 ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg atgataagca
     7621 tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata
     7681 attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt
     7741 atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa
     7801 aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca
     7861 tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag
     7921 ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca
     7981 ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt
     8041 cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag
     8101 cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta
     8161 ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt
     8221 ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac
     8281 tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg
     8341 gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt
     8401 ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc
     8461 ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga
     8521 aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg
     8581 acatagcgat ttttattgca taaaattcac ttgaatacaa aaactaatat gttagtttag
     8641 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
     8701 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
     8761 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
     8821 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
     8881 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
     8941 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
     9001 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
     9061 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
     9121 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
     9181 tattcttgct gtgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
     9241 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
     9301 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
     9361 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
     9421 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
     9481 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
     9541 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
     9601 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
     9661 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
     9721 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
     9781 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
     9841 tccaaatatt cctaatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
     9901 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
     9961 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
    10021 aggaaaacca ttcatcccga gtccagaccc actattgaca gaccttcagc ataagctttc
    10081 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
    10141 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
    10201 agaaatgttc atcaagatgg tagcaggacc aaatgccgta cgaaaaacat tacaggatta
    10261 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
    10321 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
    10381 acattgatgg atataccagt aggagaggta tggcctaggg agaggatcaa agaactaaaa
    10441 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
    10501 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
    10561 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
    10621 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
    10681 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
    10741 gaatttggat tgccgggatg ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa
    10801 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
    10861 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
    10921 tactcgattt ttggattagc tcgaatcgta aagaatcgag aagatttaga ttggttgtgt
    10981 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
    11041 ccagaaaatg atgtatatgg gattttagca gaattttgcc aaagagatcg aatcccattt
    11101 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accacataaa
    11161 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt
    11221 aacggataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
    11281 tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc
    11341 gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat
    11401 aagaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt
    11461 tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt
    11521 gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct
    11581 tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc
    11641 aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc
    11701 gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca
    11761 cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt
    11821 actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt
    11881 tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaat
    11941 acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta
    12001 agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt
    12061 tcaggtataa acaaatcaaa tacagtacaa agggactcca ccatttctct tgaataaaga
    12121 acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat
    12181 tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa
    12241 attggcagat taggtcgcaa aatatctaca gagagatcaa taaaaaagtc aatatattca
    12301 tactctgtga attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat
    12361 atttttaata gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct
    12421 tttttaaaaa aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct
    12481 gtagcagtac ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc
    12541 tcttttccag aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtactat
    12601 gctaaaaatg cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt
    12661 tgctgactga aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga
    12721 taactgactg attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca
    12781 attccttcaa aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga
    12841 ttgagacgct caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac
    12901 atatggaaat gatctgct
//
LOCUS       DAJSBC010000072        12734 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSBC010000072 DAJSBC010000000
VERSION     DAJSBC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.073"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT65_002210"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT65_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2329810.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ONT65_002211"
     CDS             complement(322..891)
                     /locus_tag="ONT65_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR2329811.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ONT65_002212"
     CDS             1077..1979
                     /locus_tag="ONT65_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2329812.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT65_002213"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT65_002213"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR2329813.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT65_002214"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT65_002214"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR2329814.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT65_002215"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT65_002215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR2329815.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ONT65_002216"
     CDS             8547..8732
                     /locus_tag="ONT65_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR2329816.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ONT65_002217"
     CDS             complement(8794..9990)
                     /locus_tag="ONT65_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2329817.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ONT65_002218"
     CDS             complement(10160..11596)
                     /locus_tag="ONT65_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2329818.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ONT65_002219"
     CDS             11889..12698
                     /locus_tag="ONT65_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2329819.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2675 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAJSBC010000073        12733 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSBC010000073 DAJSBC010000000
VERSION     DAJSBC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.074"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(287..649)
                     /locus_tag="ONT65_002220"
     CDS             complement(287..649)
                     /locus_tag="ONT65_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329820.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ONT65_002221"
     CDS             complement(723..1103)
                     /locus_tag="ONT65_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR2329821.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="ONT65_002222"
     CDS             1167..2360
                     /locus_tag="ONT65_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2329822.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="ONT65_002223"
     CDS             complement(2437..3021)
                     /locus_tag="ONT65_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2329823.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ONT65_002224"
     CDS             3222..4220
                     /locus_tag="ONT65_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2329824.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ONT65_002225"
     CDS             4332..4841
                     /locus_tag="ONT65_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR2329825.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ONT65_002226"
     CDS             complement(4871..5530)
                     /locus_tag="ONT65_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR2329826.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ONT65_002227"
     CDS             complement(5532..6296)
                     /locus_tag="ONT65_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR2329827.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ONT65_002228"
     CDS             complement(6304..6981)
                     /locus_tag="ONT65_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR2329828.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ONT65_002229"
     CDS             complement(7018..8403)
                     /locus_tag="ONT65_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2329829.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ONT65_002230"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ONT65_002230"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT65_002231"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT65_002231"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR2329830.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ONT65_002232"
     CDS             9342..10199
                     /locus_tag="ONT65_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2329831.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ONT65_002233"
     CDS             complement(10280..10615)
                     /locus_tag="ONT65_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329832.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ONT65_002234"
     CDS             complement(10682..12052)
                     /locus_tag="ONT65_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR2329833.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ONT65_002235"
     CDS             complement(12130..12666)
                     /locus_tag="ONT65_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2329834.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3791 a         2581 c         2120 g         4241 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tga
//
LOCUS       DAJSBC010000074        12430 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSBC010000074 DAJSBC010000000
VERSION     DAJSBC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.075"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1562)
                     /locus_tag="ONT65_002236"
     CDS             complement(99..1562)
                     /locus_tag="ONT65_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2329835.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1626..4643)
                     /locus_tag="ONT65_002237"
     CDS             complement(1626..4643)
                     /locus_tag="ONT65_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2329836.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4794..5633)
                     /locus_tag="ONT65_002238"
     CDS             complement(4794..5633)
                     /locus_tag="ONT65_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2329837.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5778..6023
                     /locus_tag="ONT65_002239"
     CDS             5778..6023
                     /locus_tag="ONT65_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329838.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6086..6508
                     /locus_tag="ONT65_002240"
     CDS             6086..6508
                     /locus_tag="ONT65_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329839.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6602..6919)
                     /locus_tag="ONT65_002241"
     CDS             complement(6602..6919)
                     /locus_tag="ONT65_002241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329840.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6946..7152)
                     /locus_tag="ONT65_002242"
     CDS             complement(6946..7152)
                     /locus_tag="ONT65_002242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329841.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7133..7315)
                     /locus_tag="ONT65_002243"
     CDS             complement(7133..7315)
                     /locus_tag="ONT65_002243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329842.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7329..7679)
                     /locus_tag="ONT65_002244"
     CDS             complement(7329..7679)
                     /locus_tag="ONT65_002244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329843.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7705..8007)
                     /locus_tag="ONT65_002245"
     CDS             complement(7705..8007)
                     /locus_tag="ONT65_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329844.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8010..8243)
                     /locus_tag="ONT65_002246"
     CDS             complement(8010..8243)
                     /locus_tag="ONT65_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329845.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9229..9551)
                     /locus_tag="ONT65_002247"
                     /pseudo
     CDS             complement(9229..9551)
                     /locus_tag="ONT65_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9674..10789)
                     /locus_tag="ONT65_002248"
     CDS             complement(9674..10789)
                     /locus_tag="ONT65_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2329846.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10795..11193)
                     /locus_tag="ONT65_002249"
     CDS             complement(10795..11193)
                     /locus_tag="ONT65_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2329847.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3381 a         2514 c         1883 g         4652 t
ORIGIN      
        1 tcaaattaaa aaacgaaaat gagagataca aatatatcaa atatttaaaa agtaaccaca
       61 ttttctatac cactccttat cagaaacagc cttattcttt agaataagac tgtttctggg
      121 ttgattaatt tgtcttttgt taaactacca tctaaggcaa gttctaaatg taaatgtggt
      181 cctgtactat ttcctgttga acccacataa ccaataattt gtccttgttc aactgtttgt
      241 cctacgttca cgcaatattc ctgttggtgc gcataaagag ccacacttcc gtcttcatgc
      301 tcaattgtga cataatttcc tgacaaagta tgatacgaag cttctatgac cgttcctgcc
      361 ttgctcgcat agattggttc tccttgattg gctgccatgt ctaatcctct atgaaattct
      421 tctcctcgcc aaccgaagga actagaaatt gaataatttt ttagaggtac catatagcct
      481 tctttgctta gttgtggacc agctacttct ttatcatatt tgattaagtc ataggtttcg
      541 attaacccat ttaatttttt atcgtagcta gtgtctgttg cgtatcttcc tgttaaaaat
      601 ttagtcgctt cctgataggt ttttgcctta cttttccaaa caccatcata aaaagaggta
      661 tttccaacta agccttcttt taacagtttg gcatagtctt ttaagctttc ttcatagctc
      721 tcatattgcc gaaaagttgc ttgggtcgta taaagtgtcc cattgcctaa atcttcttgt
      781 gtcgcaaaag agacgccctt tccgttatgt gtgcctttga ttccaaatag attgtagttt
      841 ggtgcctgag ccaattgact ttgtccgctt gcggattcta aaatggcttg cgcgatcata
      901 acagaagcgt ataaatcatt ttcttgtcca attttacgag cagattcacc gattttacgg
      961 ataaacgttt ctacagacgc gtctttttca aaatgaatag tcccttcgtt atcttgtgtg
     1021 ctggaaggga ctaattcttg tatcggtgcg cttggtgctt ggtggtagtt ttttgtctct
     1081 tctgaaccga ttgattgtgc ctgcggtacg tcattcatag gtacagtcac actctgttca
     1141 ggtgcctttt gtgggcgttc cgtttttgtt ggcggttccg cttctgtttc ggtacttggt
     1201 ttttgaggag tctgtgagct ttcatcagaa ggttgctcat ttggttcttc tgacggttcc
     1261 tcctcaggtt gttgattgtc ttctggtttg tctggttgat tcggtgtttc tggttgttct
     1321 tcagaacctg ttggtggagc gttcatttct ggattatctg gtgtatctat tgtatcttca
     1381 gtcggtgttt ctggttgatt ttcttctcca gtggaatttg ttgtcgtttc ttcagtgatc
     1441 gttgcagatt gcgaatttgt cagttgattg ttgccttgtt cattagccaa aaccgctggc
     1501 gtatacccaa atacttgacc tactaataac aaagcagata aacatttact cgtttttttc
     1561 acaagattgc ctccattctt caatcaatca tctagagaca gctgattgct gtctctagtc
     1621 atactttaat tgctttgttt cattttacga cgaaccagaa cagcccccac accaacaaaa
     1681 agaaacagtc ccaatgcaat taacacatcg ttcactgttt ctcctgtttt tggtaagctt
     1741 tgttttggtg tgctcgtcgg cgtttgtttg acaggggttg tcggcgtttc ttttttaatg
     1801 ccttttgctg tcaatgtttg tttttcattc ttcaaatcaa agtgacgtgc ttcttcctct
     1861 ttatggtctt tatccttata gacaatatga gttgcgacca atttctcccc gtcttttaac
     1921 gtgcctgctt tgtagtcaaa ggatacttta aactcttcag acgctttaga aactttacgt
     1981 gcagtttcta ccgtaccaac gactgtatct tttccatttt tatcaatctt atggaattga
     2041 gtcacccagt atttggtttc ccctaccgta gattctggaa attcttgcga tacatgatcg
     2101 atcaactctt ggtctttgtc tggatcaaat gtttggtcgc cattcactgt aataaagcgt
     2161 gtttcgattt ttggttcttt cgcagtcact gtttgatctt tattggttaa atcaaaatga
     2221 cgagcgaatt cttcttcctc ttctttgtcg ttatagacaa tgtgcgttgc gacaatcgtg
     2281 tcaccgtctg aaaccatgtc tgctttgtag ttgaacgtca catcaaatgt ttctttttca
     2341 tcggtcactt ttcgtgtttc ttttaccgta tctaatacag tgacttttcc atcggcagta
     2401 attttatgga attgcgtcac ccaatatttc tctgtcccaa ccgcagattt agggaatact
     2461 tgggacactt tatccactaa ttcttggtct tttttcgggt tgaaatcttt tccacctgtc
     2521 gttgtcacaa acaaagtaga aatttctggt tggtcttctt ctggaaccac gatttcttcg
     2581 tttttcacag taattggttg tacttctccg tctttgaccg ttactaaaat cgtttctgtt
     2641 tgtcctgacg cttgcccata atgctcaggg aacacaattt ccgtaaattt atacgtaccg
     2701 tctggtaatt ttagtttttc tggttcttct tttgtttcat acacaaagga cacattttta
     2761 ttatgtgcag tcataccttc aatcaggaat ttcgctcctg gtaattgtag atgattgatt
     2821 gaatcgactt tattgaagtc tacttcaccc gtttttaaca agttgtcaat cacatctaat
     2881 tcaaacgtaa gcacaggcgt cgtgttatcg ccataggcca cagagaacgt ggtttgttct
     2941 tcagaaggtt gacggtagtt gtctggcaca tttttttcaa tcaattggta tttgttttga
     3001 tcgccattgt agaatttttt gtttgccttc cagtgtccgt ctttgtcagt ttttactgaa
     3061 tccaagactt ctcctttttt cacaaggact gtttctttat ctggcgcagt aatatctgtt
     3121 ttggcgataa tatcaaattc gatattttct gcaggaacgg tatcataaac aggtttgcct
     3181 tgattccagg attcttccgc attatagact tctttgactt tctttccttc tggaatgaac
     3241 tgttgtaagt cattttttgc agttaacgtc acttctttgt ttggttcaat cgtaatcgta
     3301 tgagtttgtt tgtctagaat atacccagct ggtgctttga cttctttgac atccaattta
     3361 ttttgaacat attttccgtc tttttcatag aatgcaggga tttcttttaa ggcgatttcc
     3421 cctttggcat ccgttgtata ttgttttgtt tctcccgtat catgacgttt gatttcatac
     3481 gtcacatttg gaactttttt ccctgtgaca gcgtctagtt tggttagttt gaagtcgcca
     3541 ttttttagaa ctttaatagt tagtccagct ctattaggat ctggcacata aagctttcct
     3601 agattttgag caacaggatc tacataggca aaacttgaac ctacccctct agaatttgga
     3661 tccagtgaag aaacagaaat tcgtccgtca tttgagtttt gatttgcttt tataaacaaa
     3721 ctatttcctg attttcttac ttgaacgtta gctgtgtttt caacaaccga cccatcccat
     3781 tgagaaaaca caccttttgt atccttaact tctacttctt cccctacttt aaccgtcact
     3841 gtttggctat caaaagaagg atcttcaagt aaattgttta tttttctatc tacagtctgt
     3901 ttgaaagctt cataattaga ttgtccaata gttccataat taacaaatga accacctaaa
     3961 acttcccaaa tatatgtttg tgctaataca tagttatcca ttgaaccact agaatagtag
     4021 ccaaaataac caattaaaga aatctttttt ctaatagcac ttgtcgtaat taaattttcg
     4081 agtggtgtca catcatattg aatacccgca ctcgtgattg cgccttcttg aatgcaaaaa
     4141 acatcttctc cgtcaatttg ccagcgaacc atgtaatcat gagtatacat tgttccttca
     4201 ggagtaattc gagtcatatt cacatcttta cgaccatttt cagtcaatgt tagattggta
     4261 gcacgtgtct gtgcattctt ttccaagatt gcttgccatt cttcagattc ttgcgccaat
     4321 cgttcttgtt cagaaaaaga agtcgctttt tgaatatctt caatggttat aaagtatttc
     4381 tcataacctt cagggacctc ttgagtcatt acattgggcg gagaaacatc ttctgtcgtg
     4441 tcctgcgtag ttgtctcttc tgttgtttca cttgtggttt cagtactgat cgcttcttca
     4501 ctactttttg attcttcagt cttcattact tcttcagtag aatttgattc atttatcgtt
     4561 tctgcaaatg ccgttacaga tcctaacatg ggattgagaa atgtaacgaa aattgccaac
     4621 caagttgcca ttcttttaaa catatttttt tcgttccttt cattttattt ttcagtcttc
     4681 taaagtaatt tcttatggac ttcgacaccg aaacattttt atcctcctca aaacaaaaaa
     4741 agactagaac ttataattct agtcttcatc aaccacttac ttttctttta tttttagtag
     4801 aaatctacag tgtgtttttg tgacccgtca ctgtaaataa taacatccac cataaaacct
     4861 ttgttgtacc actgcgaatt tggatcgcta acctctgcat tagcccaatc ataggcttca
     4921 tctacagtgt taaatacctt tccactatta ccaatctctc caactacaat tgttggctct
     4981 tcaggtgtcg ttgtgccacc atttccgcct tgatcgcctc caccaggatt gcttggttct
     5041 tcaggcgttg ttgttcctcc acctgtgctt gggttagaag gagttgtttc tcctccagta
     5101 tttccaccat tacctggttg tgtaggtgtt tgagtagtgc caccattgtt ttctggtgta
     5161 cttggcgttg ttgtaccacc agttgttcct gtttgatctc cagctggtgt tgttggtgtc
     5221 gttgtatttg gtgtcgtttg tgtatttgaa gtcgttcctt gtcctactgg ttgattcgga
     5281 tcaactttgt ctttctcttg aatcactgtt tgagccacag tttcacctgc gctgttttgt
     5341 acacttgcta cattgccatt aaatactaat ttatttcctg catagattaa atccacattt
     5401 gtaatcttat tcatgtccgc taatttttgg acagtaatat ttgttgcttg actgattgcg
     5461 cttagggtat ctccccagac aattgtgtat tcattccctt taatttctgc tttgatttgt
     5521 tccgccgaac gaggtgtcca aactttgttt tctgctgctt cagcattcgt tcccacagca
     5581 agactaactc ctccgactac gattcctagt gccaatactt tcaacatttt cattgttttt
     5641 cgcctccgat ttgaataaat ttatttacaa aaacatagta ccaaacttat gtctatcagt
     5701 cgatatattt tttgtagatt tttttatttt ttgatatact aactttggta ctaaaataac
     5761 taaaaggaat gtcacacatg caacttaata atttaaatga aatacatgat tgggctcaaa
     5821 ataatttact taatcgtaaa caagctgcca aaatcactgg acaaaactat gatgcttttt
     5881 ctagctcttt acgtagaaat aatatcaaac catttctaga aataggcgac gaacctggtc
     5941 cttccatcat tcgcttatat ctaagagaag atattgaaaa atacgcaaaa cagctcgaag
     6001 aaaaaagaca gaaacgtcaa tgaggcgttt tctgtcttgg atttacaagt aaaacaataa
     6061 taaacacttt tacaacggag gttctatgaa taagaaatac attgtttcag ttggttcaat
     6121 cgtactgatt gtactcgcta ttattgcttt ttctgtatcc aacaaaacag aaacacagcc
     6181 acaaaatacg gttcaatcga ctactacaag tcaatctagc gatcattccg tctatccttc
     6241 actaaatagt ttaaaagaaa cgcatggaca agatttaatt gatcttggtt cttctcaaat
     6301 tactactgtc ttatttgaaa agaaaaatat tgcgtttatt actcctaaag aaggaactga
     6361 ccttgaattt tctatcaaag taacaaatac caaagaaaat ttattctcca ttcctgacat
     6421 tcaatatacc cctaacaagt taaagattgg ggatacgttt ggtttggcta aagtaaatga
     6481 acaatacttt gcttactcac tgaaataagt ttctaatagc aagaggaaaa accctcttgt
     6541 tatttttatc acataatcat tttataaaaa cgcttcgaaa atatcaacat ttttgaagaa
     6601 actatttttc taatttctca atttcttcca agggattttc gctaatgacc catacaggca
     6661 cagattgctc tcgaggctcc ccttcttcta accaccaacc tgcataatga acttcttcct
     6721 cctcagcatt accatgatac caaccaacaa aggattcatc tacaacgaat gaaatttctt
     6781 cttctggatc aaataattca tttttccatg cttcaattgc ttgttctttg gtgtactttt
     6841 gtttgttaaa tacaccacaa atttcgtcat ctacataaaa aatttcaaaa tcaaaattac
     6901 tcgttccttt tactggcatc ttaacttttc ctcctttgac tcacttcaat agatatacca
     6961 ctcatgttca ttccattgtt tactagttaa ttggagtttt atcttagggc tttgtgcact
     7021 ctttccaaaa acttgagcgt cgtttttttc ttctaaccaa aaaaggtaat ttcttacatt
     7081 cggtactaaa tgtttttcct gcatttcttc atgaaaaaca tataattctg cttcattttt
     7141 caaccaattc attattcatt ccctcattca cttcttcata ccgccattca gatagtgaga
     7201 tttctttttg tttctccact tgtcctataa aaatcgctgt attcaacaag ctgatcacac
     7261 ttaataataa agcaatgaaa ctaatggtta cacttactct ttcacatttt gtcattttgt
     7321 ttccttcttt actcgtttgt tatttcttgt aataggtcta ataactcctt caccgaaatt
     7381 tgcaaatggt tcgcaaaaat ttcaataaaa gcactcgcac acattaccag accttcaact
     7441 gatatatcac tgcgacaata aaattctgga gaaacactga tttctgattc tttcttacaa
     7501 ttgaacgaaa aaactatttt tgaatcaaat tccggtaatt cttccacaac tatgttttgg
     7561 gattctacca agaaagggag tgcctgttct aatgcttctt ctggtgtttg agcagataga
     7621 ataattgctt cttgttcttt tatttcattg ttctcaaata ctttataact ttttttcatt
     7681 gttagttcct ctttcattcc tctctcaatt agctaagccg ttactggtac gccattctct
     7741 aaatttagta tcgctaatcc ttaatgctcg tcgaatatca atcattcgat aacctattgc
     7801 acgaagttct ttatatcttt tagcagttag cgtctctaac gtcatttcag gcaatgggtt
     7861 cgaatcaaaa atagaattcg aaccgattat ttgggccaat tgttgtactt gtaacagctc
     7921 ttctgaactt aattcagtga ttgctcgatt gcctatcatt gcatacattt tctgtcgaat
     7981 atacttcttt tcttgtttag acaacatttc tatccactcc tattttcatt atcatttgtt
     8041 acctccactt cagtaaatcc acaaataaat gaattcatat tccaagtacc attcccttca
     8101 actacaactt ttctcctgtc ctctttcgga tattcaagaa tcagaccgtt taccaatatg
     8161 tttttcacta ctactaattt gtttgtatac cgtggaattg ctgttccgat atacttcact
     8221 ttatttccta cgctaattgt catttcctca ctcctattct tttttatcca aaactttttc
     8281 ctgtcctatt cctgccaacg aatatacttc actgcatgct taaacaaaac aaaatgttgc
     8341 cctgttttat ttccgtcttt tacttgttcc acatacagtt catatttggt cacacctttt
     8401 aaaacgccag atactttttt tcatccattg tcacaattag ttttttcatc aatcgattga
     8461 tactgcactg ctcatttttc ttgttcatat agtattcctt tctttttcaa aactagcctc
     8521 ttttgtttac tagttttcat caatcatcaa cgatttaaca gattttttct ttcgcagggt
     8581 ttctagcgtc agcaacccca aactctaaaa aagtgccttt gctcccttga aaaacgagcg
     8641 gacgattgta ctcgtcatca tatgtttttc atactacctg atccacgcaa ataacctttt
     8701 tttgttctgt gccacaccca tccatacgta tcgcttgaga ttcaccatac ttttttaggt
     8761 ggtttatcct tcctaagtcc ctgttagaca cgctgattaa cttagaaagt cctgtttgta
     8821 tccaattttc aaagatctcc caaaagaaat aagtaggttt acgtgtatcg cttctgttca
     8881 aagtacctgt ttgtacttga gtatttctac tcccctcgtt caagggattc cttccaccag
     8941 ctagcttttc ctttcatcat caaatgttcc tgaaaagcat ttacgtttcg tgtccgtgtg
     9001 cttacttatt cttttttcga tagcgattat ccgctcgcta ctcgctattt tatactttgt
     9061 ttgttagctc aaataaaaac taacaaacaa aaaaggctag tttctttccg ttttattttt
     9121 ttaactgtcg ataaatagtt gttagtttaa ttccaagttc taaaaccaat acgcccaaca
     9181 aaaccacagg aaaaacaatt ggggcaaaca agtaaaatcg gatagttttc atgtcttccc
     9241 tctttctttt tggtgtcgct gtggtcgttc ttgccgttca atacttcgat acatttttta
     9301 ttcattattt gctcgttttg ttttcctctg gactgctttt cttgcttttt gaatacttta
     9361 tacgcttttc gtgcctgaat taataggtct tgtcggcgtt tcattcgtga gaaaacaata
     9421 tttgaaacca tcaaatcctg gttcgaacaa ccacgattcg caataatttt cccatcaaaa
     9481 aaaatagttt tcacttgaga aaaaaacagt tggaaatctt gttttgttgc taattttttt
     9541 gtcaaaatca ctgtgctttt ccctgttgaa tctacaatga ttcttcgaac aatccgattc
     9601 gataacttcc aacgttgccg attaaacaat gttacttcac aacaaatatg acgtaagtga
     9661 ttaggggtca ttttcatgtg tccaattcct ccaaccaatt atgcatcgaa attttgggca
     9721 attgtgacag cccataaatc tgcgtttcca ggtcttcata actacaagaa cgcttggctt
     9781 gaagtaatga atattctgct tgatctgata gttttgttgc cattggatct agattcaatt
     9841 ctcttacaca acattcccaa aaattcaata gcgctctaaa ataaaatcct tttcgattct
     9901 gaattgcttt tccttcatga cgagcaattt tttcattttg aatacaccgt tgatacgttt
     9961 tgcttaaatc ttctgcaaat gtttccccta aaattcgata ctcgcttcca tcagataaac
    10021 catgtgtttg attatacttt cgttgccgct tttctataat tcgtttactg tcgtatatca
    10081 tgtttagctg ctctttcgct tcacgccatt cagctgaact gcgaacaatc catgtcaaaa
    10141 catctttcgg aacaaacgta ctttcgctgt aatgagcaat aaactctttt tgccaatcat
    10201 cagccatttc aacaggacta gtcgtatcga tttttgtatc tagattttct gtatctagat
    10261 tgttagaaga attattaaat aattgtgcag aattttcaca tggatttttt tcgtcaaaat
    10321 catcgaaaat cgctaaatta tcaacagatt cctcctcttc ttttccttgt gcagaatcta
    10381 cactggttga gtcaacagga cctaatgaat acacatcatt ttctgtcgta tctaattctc
    10441 ctagatacaa ccgagctttg tttctttttc ctgtttgttg atcaaatccc atatcctcta
    10501 tttctagtag ctcatattct ttcaattctt ttttgattgt attaacagaa cgtctgcaac
    10561 aattcaatgc attggctaat tcttgttccg taaattcaaa ataaacaaaa ccttgactat
    10621 ctacttgatt tcgttgaatg gctaaatcta agcgtgaccg aaaaaccata tacgcgattt
    10681 ttgctttatc agacaatgag cgatattttt cacttgtaat aaataccttt ggaaattgat
    10741 agtataattc attgtattta cgagttgcgg taaatctttt tgttgccatc gttattacac
    10801 ctcatcttgt ccatatatta agaaagaatt ttggtgttgg tagacacgaa catcttccat
    10861 tgtagcaatc aattctttta gataaattcg accgaattca attgttagta agtcgtaatc
    10921 ttctaattca tttttgattt cttcaatttc ttcttcagac acattgagta aaatggctaa
    10981 ttcttgattg gtataagaaa tatagacatg tttcttttca tcaatccgtt tttcaattaa
    11041 ggattcttct aagtactcat tcagtaagct ataggcgtat tttgcttgtg cagtcatcgc
    11101 ataatatttt gacgatttta taaagaatag ttttggcata tgaaaagttg cttgtgtcag
    11161 cacatctgtt gcgcgaaatt tgttttttac catatttttt cctcctagaa aaacacgcaa
    11221 aaaaagctct gtataaatcg aattacacag agtttcttct tgttgatatt taatttctaa
    11281 aatacgtttc gggtaaaaac taaacactta ctaatattta gattgtagaa tcttcgcctt
    11341 catttattca ttttaaagag aaaaagatca agtgaaatgt gcccaaaagt ttgattctat
    11401 taatttttaa acaccattca taatatattt cctataatgt aaaaagcgtc gatacttatt
    11461 aaagcatcga cgttattttt atagtaaatt tatcaaaatt tttgccaaca ttttttccga
    11521 tttttttccc tatttctatc tttccttatc tttcttagtt aatattattt gatgttaata
    11581 tatcactact aatattaaaa aacattgatt tatcatagga taaagagtga tagtttaata
    11641 cttaaagaga catattaatt ttatttaaaa attgacatta atgactctta atcagcgggt
    11701 cgcgggttcg agcccctcac ggcccattgg gtgccaaacc cacggaacag gtaaatgttg
    11761 gtgcgaaagc atttcgaaaa catttacgct gttctttttt tatttttcaa aataactagg
    11821 gctttttatt tttaactcaa agtccactgt agccctttgg actttgagtt aaaaaagaag
    11881 cataattatg aacagtaaag tttctaatat aacaataatt cgagaagctt tgcaaaatca
    11941 tgctgccaat gaactctcta aacatatagg attaaatcta agtactataa aaggttaaaa
    12001 agtgaagaat atttaataga aaaactaaat ttacttgctg ctttcccctt ttccattaat
    12061 acaacaatca gagagtgtct ttttttcgaa aaaattagaa taaataaaag acacgtagtt
    12121 tttttatcta catgtcttcc atcgactaca agttatcatt tctcaaaacg tatttagctt
    12181 ttaattgatt aatcgtttca cgttgtctac acattgccta attaagattt tcttattaga
    12241 agcatcaatc ccgcaataat acctaaaact ccaggaatta cccacaataa tgttaaaaga
    12301 aaaaatggaa taatagataa aatgagtaaa ctaataccat agaccactgt tttttgattt
    12361 tttcttacta aaaaacctat tatgaaaatc attatcgaaa aaactagggt aattcctcca
    12421 aataaaacaa
//
LOCUS       DAJSBC010000075        12307 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSBC010000075 DAJSBC010000000
VERSION     DAJSBC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.076"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            287..694
                     /locus_tag="ONT65_002250"
     CDS             287..694
                     /locus_tag="ONT65_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329848.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ONT65_002251"
     CDS             708..1109
                     /locus_tag="ONT65_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329849.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ONT65_002252"
     CDS             1111..1482
                     /locus_tag="ONT65_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329850.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1817)
                     /locus_tag="ONT65_002253"
     CDS             complement(1518..1817)
                     /locus_tag="ONT65_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329851.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1823..2053)
                     /locus_tag="ONT65_002254"
     CDS             complement(1823..2053)
                     /locus_tag="ONT65_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329852.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2670..3332
                     /locus_tag="ONT65_002255"
     CDS             2670..3332
                     /locus_tag="ONT65_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329853.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3411..6636
                     /gene="fss3"
                     /locus_tag="ONT65_002256"
                     /pseudo
     CDS             3411..6636
                     /gene="fss3"
                     /locus_tag="ONT65_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6750..7064
                     /locus_tag="ONT65_002257"
     CDS             6750..7064
                     /locus_tag="ONT65_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2329854.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7077..7451
                     /locus_tag="ONT65_002258"
     CDS             7077..7451
                     /locus_tag="ONT65_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2329855.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7461..7805
                     /locus_tag="ONT65_002259"
     CDS             7461..7805
                     /locus_tag="ONT65_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2329856.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7876..9228
                     /locus_tag="ONT65_002260"
     CDS             7876..9228
                     /locus_tag="ONT65_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2329857.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9288..10430
                     /locus_tag="ONT65_002261"
     CDS             9288..10430
                     /locus_tag="ONT65_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR2329858.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10455..10709
                     /locus_tag="ONT65_002262"
     CDS             10455..10709
                     /locus_tag="ONT65_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329859.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10965..12149
                     /locus_tag="ONT65_002263"
     CDS             10965..12149
                     /locus_tag="ONT65_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2329860.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12146..12283
                     /locus_tag="ONT65_002264"
     CDS             12146..12283
                     /locus_tag="ONT65_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2329861.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3476 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgtaggat cgaatttaaa ccataattgt
     1801 gtctcattaa aaagcataac cattactccg atctgttttt aaaattagtt tctacctcta
     1861 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
     1921 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat
     1981 cttttttgct gctttgctca taaaagtctg catattcatc actgatattt tttctaataa
     2041 agtcttccat cataaaaatc acctcaaaac gattatacga acttacgttc tcgttgtaaa
     2101 gcgatatttg aggggcaaaa agggggcaaa aatacgttta tctttgatat atctcagatt
     2161 gaacatagtg tttattatat gtcggtattg ttgatttata gctgttttga gggtattaga
     2221 tacataatag aatatagtgg ttttgtttac gtgcattaca cggaaaagct gctcgtatca
     2281 aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc
     2341 ttgtatatca agggcttttt cttttttctc tatcacagtt ttaagccatt tttggctgtt
     2401 ttggaccatt tttcaatcat attcccttaa aaattgtcct agattgtccg acattgacta
     2461 tcagtgtcgt caaaataggg caaaatttag tagtcagctt gacgacaaat ataatacgaa
     2521 aaataccaaa acatttaccg aatttagcaa cttttgaacc caagtttctc aaagggtcag
     2581 ctattagcaa aaaagacaaa acatgtactt ttctactaaa aagttgatgt gataggcttc
     2641 tatctaaata actattagga gtttttatcg tgaagcaaaa tgatggacgt atcatttgga
     2701 aaaatcaaag tgagttaaaa ctgattttaa caattaatga attcatcgaa aaacacggaa
     2761 ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa tccaaattct gcaccaagta
     2821 tgtggttcat taacaaaaaa tatggatcat gggagaattt attgattagt ataggacgag
     2881 aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca agaattattg gaaatagtag
     2941 aagcctttat taagtgcgag aagataacct ctcaacgaat gtacgaacaa aaatcagtag
     3001 ggaagaatat tccttcactt agtaccatta aaaagatgtt aggagatata cgtccacttt
     3061 ttaaagaaaa aaatgatgga tctcgattta ctgatttcga actgctgtta gaactaaaaa
     3121 atgaaataat ccgattaaaa ctacaggatg atttatcaat gacaaagttt cgaaaactag
     3181 ttcaatcccc aaaactacca tcagttgata caattatgaa aagaacgaac aaaaattggg
     3241 aggaattgat ggcagagatt ggatttgatt atcgaagaat caaaatttat aagcagagaa
     3301 acaacctatc caagacaaag aaaactaaat agtatcactc taaatcagac gttcaaaatt
     3361 aaaatttgga cgtctttttt attacccaaa aacgaaagga gatttatttt atgaaaaagc
     3421 caagatttaa gaattggcgt ttgctcgcaa cactgagtct attatgccaa acaattggtg
     3481 gatcgcttgg cccaacgatt gcctttgctg atgaaattac tcatccacaa acggtaactg
     3541 tggaattgga tttggctcac cagtatgctg tcgaaggtac atttagcgat ggtcgtccca
     3601 tgtcagaagt taccgttccg cactatgcag tttacaatgg tgtgaagcaa gatattttct
     3661 gtattgagcc aggggtgccg atttacaatg agttcactcc tggttatgag aaaaatccat
     3721 tgcctgatat gtcggagaaa gcgaaattag tttccgtttt atggaaggaa gctggtacgg
     3781 atgtggatac acatattgtg gcccagaaga tgatttggca agaagttaat ggctacgctt
     3841 cactcaatta aacgttctga tggtagtgca gtaaatatcg cagccattga gtcaaaaatc
     3901 aatcaagcca ttgcggatta tcaaaagaaa ccaagcttcc ataactccac agtgaaaacc
     3961 gtgttaggtc aatcgactac tgtgacagat acgaacggct tgaatttatc ggagtttgat
     4021 gaagttgtcg agaatatcgc aaatattgat tatcgtgtga acggcaatca actagtgatt
     4081 actccaaatg ccaattcaaa agagagtggt gaattaaccc taaagaagtc agctaataca
     4141 ggaacaccgg tagcttataa aatggctggt caacaaactt tgatggcggg ggcaattgat
     4201 aagcccaata cctacacaat caaaattgat gtggaaactg agggttcttt gaagattaaa
     4261 aaagtcgata aagaaacagg aaatgttgta ccgggaaccg ttttccattt agactttgga
     4321 aaaacattac ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac attggatgag
     4381 attcctcatg gcgcgaaagt aaccattact gaaaaatcag tgccagctcc ttatacgatt
     4441 gacactacac caatcaccgc aaccattaaa tctggtgaga ctatttctgt gacttcaaaa
     4501 aatactcgcg aaaaaggtca aatcattttg aataaaatgg gggtggaaac aggaactgat
     4561 ctttggaacg acaaatactc attagccgga aatacgtttg ccattcgcaa agacagtcca
     4621 accggtgaaa ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga aacaccaaaa
     4681 gagattgcca atgcgttgga attgggaacc tattatgtga ccgaaaccaa agctagtaat
     4741 ggctttgtga atacctttaa accagtaaaa gtcgaattga actatgtcaa tcaaaccgtg
     4801 gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga aaccactttg
     4861 acaaaagaag acaaagatac cggtaatgaa actcaaggta aagctgagtt taaaggagct
     4921 gaatatactc tctttactgc aaaagatggt caagcggtca aatggagtga ggcttttaaa
     4981 ccagagttag tgaagggaac gaaagcttct gatgaaacgg tgactttgac tttagatgaa
     5041 aagaatcaag ttgctgttaa acacttagcg attaatgagt attactggca agaaaccaaa
     5101 gcacctgaag gatatacctt ggatgaaaca aagtaccctg tttccatcaa aaaggtggat
     5161 gataacgaaa aaaatgccgt gattacccga gatgttacgg caaaagaaca agtcattcgc
     5221 tttggctttg atttctttaa atttgctgga tcagctgctg ggactgccga aactggcttc
     5281 aatgatctga cctttaaagt atcgccattg gaaggaacca gtgagattac aggtgccgaa
     5341 gatgaagcaa ttacagctta taacgagcaa ctaggttttg atggctatgg caagtttgaa
     5401 aatcttcctt atggggatta tttacttgaa gaagtagagg ctccagaagg atttcaaaaa
     5461 attacgccat tagaaatccg ttctactttt aaagaaaata aagcggatta cactaagagt
     5521 gaatacattt ttacgattac cgaaaaagga caaaaacaac caattaaaac ggtgaccgtt
     5581 ccttacgaga aactgacgaa taaagcattt tctgttagct tgaatcgttt gatgctctat
     5641 gatttacctg aagaagaaga tagcttaact tctcttggga cttggaaaga cggaggcaaa
     5701 gaattgacag accttgattc taccgagcta gttgataaat tgagctataa cttgcatgaa
     5761 atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggaagcgac aaaagctgcc
     5821 caagaaaaag atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc aacgttggcg
     5881 aataaagaga aaacaggaac ttggaaaatt ttgcataaat taaccgcaga acaagttttg
     5941 gataaaacca tcgtcttatt caactatgtg tatgaaaaca agaaacccta tgaagcaggt
     6001 gatgatccag tagcgaaaga tgctagcttg aacaatcaag cccaaactgt caattgtacg
     6061 gtggaacgcc atgtttccat ccaaacaaaa gcccacctag aagatggttc gcaaaccttt
     6121 actcatgggg atgtggtgga tatgttcgat gatgtgtcga ttacccatga tgtactggat
     6181 ggctcaaaag aagcttttga aacaattctg tatgcattac ttccggataa tacgaccaaa
     6241 gaaatttgga aatctggcaa aattgattat gaagtgaatg acaaagaatt cactaaaacc
     6301 gtacttgcgg aaaaagtgga cacaagtaaa tatccagaag gtacaagttt taccttcaaa
     6361 gaaattaact atggcaaaga cggaaacatc aatgggaaac acaatgagga tctaaaagaa
     6421 aaaactcaaa ctttaacacc taaaaaagtt ccgacaacac caagtgcacc agaacaaccg
     6481 gaaataccaa ctgttccaag tgactctcaa gaatctagtc ctacagtaaa gacattccca
     6541 caaacagggg agaaaaattc taatgttctg ctgtttattg ggtttacctt gatctttgcg
     6601 actgctgggt attatttctg gaatcgccga aactaaggtg atgtgatgaa acaactaaat
     6661 aggaaaaacc aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat ttttgtatcc
     6721 aaaactaaaa atgaaaagag gaaaagaaga tggaattgaa atttgttgtg ccagatatgg
     6781 ccgaaacctt tggaaagata aggtatgccg gcgaaggcga agttttaact gaaggatatg
     6841 ggcggaatac tacagtaatt ggtcgtagtt atcatttgta ttccagtaag caacgagccg
     6901 atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgat caagatcaac
     6961 cactaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt
     7021 ttaccgacta cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa aagaagatga
     7081 gattaacaga aggtattgtt gtagattcag gactaacgtt tgggaaatta cgattttccg
     7141 cattacgccg agaagtacgg aagcaaaatg aggatggaac ggttagtaac gaggtaaaag
     7201 aacggactta taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt agtatcccag
     7261 caaatgtacc cttacgcgag tttgcctatg acgcagaggt agagttggtc aatccaatca
     7321 tggatacggt cgccaattat gttttccgag aaggaactac tgtcaattgg ttcattaagg
     7381 ccgatgattt agtcttgaaa cgacaaccag atcagggaaa ttctacaaac caaaatgaag
     7441 gaaagaaata ggtgaagcgt atggaaatta atgttgaaca tattttagat tgtctcgatc
     7501 aatatggaaa aggtgagcta acggaggaac aactcactaa agcattaacc tatgatgaaa
     7561 aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgcc gaagaagatt
     7621 ttgatgtact gaattggtta ggggaagaag agtccttctt tatggttatc gaagtaaatg
     7681 aaagtctatg tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa atgccggatg
     7741 ccattgaagt ttttttaaag caactagtgg aaacgaaaca actccctata gcagtaaatg
     7801 actagcataa ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt tcgattggag
     7861 gtggaaacca taaaaatgta caaagggcat cgtatacggg caggagatca gcacttggtt
     7921 tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat
     7981 tttcaagagc ttagacaatt tgatatttct aagctatcac tttctactat agaaatcgtt
     8041 cgttctataa aagatttaat ctatttgcta ggaagtctcg ttctttcagg aagcacgatg
     8101 ttgctttata tacacttttt tcaggatcat tggcgcagtt tgtggcatcg acaaaagcta
     8161 gcccggatga ttctagaaaa tcattggtac gaagtaaaac aaactcagag tgaaggattt
     8221 ttcaaagacc tcaatagtag tcgaaccaaa gagacaatca gttacttccc caaaatctat
     8281 taccgtatga aggatggttt actttctatt cgcgttcaaa tttcactggg aaaatatcaa
     8341 gatcagcttt taaagctgga aaagaagtta gaaagtggtc tatattgtga actagtagaa
     8401 aaggaactca aagactccta tgtggagtac actttactgt atgatataat tgccaatcga
     8461 attgggatag acgaagtagt ggcagaaagt ggggctttgc gattgatgaa aaatcaagta
     8521 tgggcttatg attctttacc tcatatgtta atcgctggcg gcacaggtgg tgggaagact
     8581 tactttttac tcactatcat cgaggcttta ttaaaatctg atgccgagct ttttgttctt
     8641 gatcccaaaa atgcggactt agccgattta ggtacggtaa tgcctcatgt ctattcccaa
     8701 aaggaggaaa tttctgcttg tgtggaagat ttctatgagc gcatgatgac tcgtagcaag
     8761 gcaatgaaag aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct tggacttcca
     8821 ccaaactttt taatctttga tgaatacgtg gtttatatgg aaatgttaac gacgaaggaa
     8881 agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca atctggtttc
     8941 tttctgattc tagcttgtca aagaccggat gcgaaatatt taggagacgg gattcgcgat
     9001 caatttaatt tccgggtggc tttgggtcgt atgagtgaac tgggatattc catgatgttt
     9061 ggcgaggttg ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta tgtagatacg
     9121 ggagggagtg tgattagtga gttttatacg ccacttgtac caaaaggata tgatttctta
     9181 agggaaatta gcgacattgg tggattaaac gttaatacgg aaggataaaa taatagtatg
     9241 tagcctagga gtgtcaagaa tcctgggctt tttttacgag gtgatttatg acatttttag
     9301 atttattcgc tgggattggt ggcttttgct taggtatgga acaagcgggt caccagtgta
     9361 tcggcttttg tgaaattgat gattttgctc gccagagtta taaggcaatc catgatacaa
     9421 gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt caatctctcg
     9481 gaccagtgga tatcctttgc ggtggatttc cgtgccaagc tttttcaatt gcgggaaagc
     9541 ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccgcta
     9601 ttctccaacc taagttttta ttccttgaga acgtccgggg attactcaat cacgaaggag
     9661 gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgat gtggaatggc
     9721 aagtgcttaa ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc cttatcggac
     9781 attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggatcgtct ccaacatctg
     9841 atcaaaacat cagaaactta ttaaatatca acccttccaa tcgaggaatg gggggacaag
     9901 tctatgggtc agatgacgta gcgcctacct tgaccggtga tgaaggaatc aaaatcgcat
     9961 tgccggtaga tgatggaatt tctgtagcgg gtatgttact gggaaatttt gaacaaggca
    10021 atcgggttta tgagataact ggaacagcgc ctactttgtc aacgaaacaa ggcggaacga
    10081 agatcatgct tcggaaaaac gaaacagaat atcaagaggt gaagccaggg aacagtgtga
    10141 acctggcttt tcctaattcc acaagtcgtc gaggccgtct gggaaaacaa agtgtgcata
    10201 ccttattaac aggagaccaa caagcagtcg tcacggatca gtatcaaatt cgaaagttaa
    10261 cacctaggga gtgctggcgg cttcagggct ttccggactg ggcttttgat cgagcttctc
    10321 aagtcaattc agatagtcag ttgtataagc aggctggaaa ttctgtaaca gttccggtca
    10381 tttttgatat tgctagaaga ttaaaggagg taaaaacaga tgatttatga tgagttgatt
    10441 ggggaatcta ctgggtgatt ggcaagattc agtcagatcc ggaattggaa gaagaactgt
    10501 accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccctgatgat ccgtatgtaa
    10561 ttgatgagga aacggatgct cgaatggagc tgaatcaagt catcagtgaa ttggaacgaa
    10621 ttgcggactt tgctaaagaa cctgatatcc gccaatactt gtttgagata aaagcagagt
    10681 tggaaattga gggtattacc gcggagtaac ggccaaataa accactgata ggcactgacc
    10741 gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg cctgtttatg gacggcgtta
    10801 gccggccatg atgtctcacc ccccgtatct aacagggggg tacaaaaaca caaaaaataa
    10861 gcaagcaacg ataaggaatg gctgtcctat caaggataat aagcaatttg tgtagatgtt
    10921 aactgatttg aaaaagttaa caccttaaat ttggaggtga gaacttggca caaaggaatt
    10981 tagattatcg gctattaaaa gaccggcgta atgaatatgg cgtttcacaa aataaattag
    11041 ctacgacttg tggacttagt cgcccgtact taaatcagat agaaaatggt ggcgtaaccg
    11101 catcgacaaa aaccatgagg aaaatttttg accaactgga aagtttcaat cccgagttac
    11161 ctttaacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat gcgaggaaaa
    11221 tcattcaaga gattctccat ttgaaatttg attatatgct ccacgaagat tacgcctttt
    11281 actcctatca agaacaatat gttatgggag atattgtggt gatgttgtca catgaagaag
    11341 ataaaggcgt ccttttagaa ttaaagggtc gtggttgtcg gcagtttgaa acttttttac
    11401 tcgcccaaaa gcgtagctgg tatgactttt tcgaagattg tctaaaagcc ggtggcgtga
    11461 tgaaacgctt agacctggca atcaatgatc gagttggcct gttagatatt cctgatttaa
    11521 cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa agctatcgtt
    11581 ccggggaact gttgaaacct gatgataaag atggtatggg caatactttg tatattggca
    11641 gtcttaagag tgaggtctat ttctgtttgt acgaaaaaga ctatgagcaa tatatcaagt
    11701 tagggattcc tttagaccag accgaaacga aaaatcgttt tgaaatccga ctgaaaaatg
    11761 atcgagctta tcatgcaatt caagatttgt taaaaggtcg cagtattgaa agtaccacat
    11821 tttccattat taaccggtac ttgcgatttg cggataaggt ggaaggcaaa aggcgaacta
    11881 actggccctt gaatgaacaa tgggggcgct tcattggacg gaatcagaag gaaattcaat
    11941 taacttctga accgaagcct tataccattg aacgaacgtt gaactggctt ggacgacaag
    12001 tcgcccctac gtggaagatg gccaaggagt tggatcggtt gaatcagaca acgtacattc
    12061 aggatatggt aaataatact cgactatcag accggcacaa aaagattttg gagcaacaaa
    12121 gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtgatggaaa ttgtgaaaga
    12181 tattttattg gtgtttggtg gcagtatgct tggtgttact gtgatgtgct tgatgcatgc
    12241 aagtgcagcg gcggaccgag caatggaaaa gaaaggaaag tgatgaaatg aatttcggtc
    12301 aaaattt
//
LOCUS       DAJSBC010000076        12239 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSBC010000076 DAJSBC010000000
VERSION     DAJSBC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.077"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(98..442)
                     /locus_tag="ONT65_002265"
     CDS             complement(98..442)
                     /locus_tag="ONT65_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329862.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(518..1765)
                     /locus_tag="ONT65_002266"
     CDS             complement(518..1765)
                     /locus_tag="ONT65_002266"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR2329863.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(1762..2568)
                     /gene="proB"
                     /locus_tag="ONT65_002267"
     CDS             complement(1762..2568)
                     /gene="proB"
                     /locus_tag="ONT65_002267"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR2329864.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            2798..3325
                     /locus_tag="ONT65_002268"
     CDS             2798..3325
                     /locus_tag="ONT65_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR2329865.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            3388..4758
                     /gene="radA"
                     /locus_tag="ONT65_002269"
     CDS             3388..4758
                     /gene="radA"
                     /locus_tag="ONT65_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR2329866.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            4792..5949
                     /locus_tag="ONT65_002270"
     CDS             4792..5949
                     /locus_tag="ONT65_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR2329867.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            6119..7579
                     /locus_tag="ONT65_002271"
     CDS             6119..7579
                     /locus_tag="ONT65_002271"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR2329868.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    7576..7815
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7901..8437
                     /gene="cysE"
                     /locus_tag="ONT65_002272"
     CDS             7901..8437
                     /gene="cysE"
                     /locus_tag="ONT65_002272"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR2329869.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            8434..9846
                     /locus_tag="ONT65_002273"
     CDS             8434..9846
                     /locus_tag="ONT65_002273"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR2329870.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            9843..10238
                     /locus_tag="ONT65_002274"
     CDS             9843..10238
                     /locus_tag="ONT65_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR2329871.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            10231..11091
                     /gene="rlmB"
                     /locus_tag="ONT65_002275"
     CDS             10231..11091
                     /gene="rlmB"
                     /locus_tag="ONT65_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR2329872.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            11095..11625
                     /locus_tag="ONT65_002276"
     CDS             11095..11625
                     /locus_tag="ONT65_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR2329873.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            11702..>12239
                     /locus_tag="ONT65_002277"
     CDS             11702..>12239
                     /locus_tag="ONT65_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2329874.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVK"
BASE COUNT         4013 a         2303 c         2666 g         3257 t
ORIGIN      
        1 aaaaagaggc tgtgacaaaa tctttgtcac agcctctaaa aagtccggca gttttttgcc
       61 gatcttcaag gcactctact tatcgtacgg atatttttta gcggaaagtc cgattcaagt
      121 ggtttttatt cagctgatat ttataagata agtgaaaaat cccagcaatc agtagcgtaa
      181 tcgtataagc gattgaatga tcgaaaaaag aatagtaatt gattgtcgct aataagcata
      241 atgcaaccat gtaactaatg tgcaagcgtg taaccagccc tttttgcccg attttgtcaa
      301 aatactgcga ccatttttcc cgttgactcc ctatgcttcg ttttttgaat tgaaagaaaa
      361 gggataaaat accgactccc agaaaagcag cgatattcag agaaataagg taaataaaaa
      421 aaatcattaa aatttcaaac atattgacac ctttctagtt ggatagaacc tttacagaaa
      481 aaaggcaaaa ggcgggaata atgtttcaca cgaaacatta ttcgcggatt tgtccatcgc
      541 cataaatgat atattttgtg gaagttaatt catttaaccc cattggacca cgggcatgca
      601 acttttgagt agagatacca atctctgcac caaaaccaaa taccgatccg tctgtaaaac
      661 gagtggaggc attgacatag acagccgccg cgtccacttc atttaagaaa cgttgactag
      721 ccgcataact atctgtcacg atcgtttctg aatgtttagt attatgccga ttgatatgtg
      781 caattgcttc atccaaagaa tcgaccactt tgacagcaag gatataatct agatattcag
      841 tatcccaatc tgtgtcttct gctaaagcag ccgtctctag atattctaac gcacgagcat
      901 ccgcacgtaa agagaccccg tgttctacca gttctttttc aatagctggc aagaaaaaag
      961 gtgcgatctc agaatggatc agcaatgttt ctgccgcgtt acagacagat ggacgggaag
     1021 cttttgcatt gacgatgatg ttcactgcca tagataactg tgcttcttta tcgatatata
     1081 cgtgattatt tcctgtacct gtttcaatga taggtaccgt tgcctgttct ttgactcgtt
     1141 ggattaattt tgctccgcct cgaggaatca gcacatctaa gtattctgtc aatcgcatca
     1201 actgtcctgc agtttcatga gaagtatcat cgacaaactg gatggcataa ggagattcgc
     1261 cagcactgac cagtacttgc tgcaataatt ggactaataa ttgattcgag aagaatgctt
     1321 ctttgccacc gcgaaggata acggcattcc ctgatttgaa tgctagacta gctgcatctg
     1381 tcgtgacatt tggacgagat tcgtaaatga tgccgatcac gcctagtggc acgcgctttt
     1441 tgccgattct caatccgtcg gtgttcgtcc acatctcatc tacttttcca ataggatcgt
     1501 ctaacgaagc aattttttcg atctctgtag ccatggcatt gattcgctca ggtgtcagct
     1561 gcaagcggtc ttgcatcgtt tcactgatat tgttttcgac tgctttttct aagtctttat
     1621 gatttgcttc aatgatcgct tgttgattct ttttgatggt ttgtgccatt tgaacaagta
     1681 gctcattttt gcggtttgta ggcataagtg ctagttgttg cgcgctatct tttgcctgtt
     1741 gtcctaattg catcaagtca gtcatttcaa gtcctcctta aataaagtac ctacttcttt
     1801 tcctgcaaga atatcaaaaa taattgccgg attttttcca tttgctaaaa tcattgcgct
     1861 gtttgcttca aatatcctag aagcggcttt taatttactc tgcataccgc ctgttccaaa
     1921 tgtgctacca gcaccacctg cttgtgccag aatcttttca tcgattttat cgatatagcg
     1981 atacatctga gcctcgggat ttgtagtcgg attatctgaa taaaacccgt cgatatcaga
     2041 aaggacgatc aaaaggtctg ccgcgacaag ttttgccatg atggctgaca gctgatcatt
     2101 atcgccaaat tttgtcagat gatctaattc atccactgct acagtatcgt tttcgttcac
     2161 gatagggatc gtgtccataa tcaaaagctg ttccaatgta ttcctcacat ttttacgact
     2221 ttcaggataa tctaccacgt ctcttgtcac gaggatctgt gccgtctgtt gattatatac
     2281 actgaatctt tgattataga tattcatcag ttcggcttga ccaacagccg ccgcagcttg
     2341 ctgttcagga atcaaagtcg gacgttctga caaatgtaat ttatgcatac caacgcctac
     2401 agctcctgat gagactaaga tcacttcctt tccccgattt ctaagatcag aaaggacaaa
     2461 agctaactga tcgattgccg caagattgat atttccattg ggataaatca atgtacttgt
     2521 tcctactttg acaataattc gttttgcatt tttaatttct ttacgcatgt gacacctcaa
     2581 tcaaatatcc actcattaat ttcgttctat tctacttgtt ttgacgagta aagtcatgta
     2641 aaaaatagtt tttttgtaca acttgcagtc gtttggaaaa agaacgaatt aatggtttac
     2701 tttaagatag cagaatttct tgaagaattt gtaaagaaag tcttttacag cctcggatat
     2761 gttagaatag gagtagttta tttagggagg aaaaccggtg agaaaaagag gatttgaagt
     2821 tatttctaaa tataaagatc aaaatatcaa tctaccgcag cgcgctacac atcatgctgc
     2881 tggctatgat tttgaagcag cagaagaggt cgtgattcca agtatctgga aagcaggctg
     2941 gaaaaagggg ttggaaacag tcgcagatgg attgaaaaaa gatgtatcag aaaatgaaaa
     3001 acaattgaaa ccaaccctcg tgcctactgg aatcaaagct tacatgggag aagatgaatt
     3061 tttgcagctt gctaaccgtt caagcaatcc gttgaagcgg tttttagtgt tgacaaacgg
     3121 agtaggtgtg attgacagtg attattacaa taatgaggca aatgaaggcc atatcatgtt
     3181 ccaattcacc aactttggtt tcaaggatgt agtgatcaaa aaaggcgagc gtatcggcca
     3241 agggatcttt ttgccgtttt taaaagcaga tcaagatcaa acgactgctc aacgtcaagg
     3301 cggatttggt tcttccagta aataaaacgt taaagatact taagcttgat ttaatgtctc
     3361 atgattagga taaaaggagg cggtacaatg gcaaaaaaat caaaagtaca atttgtttgc
     3421 caaaattgcg ggtatgtgtc gcctaagttc ttgggacgct gcccaaattg cggaaaatgg
     3481 aatacgatgg tcgaagaaat cgaacaagat acaacggacc gcagaactcg gacaagtttg
     3541 acaggagaaa aagcaaaacc aacaaaaatc gcagacgtcg tccctaaaaa agaacctcgg
     3601 atcaaaacaa aattagaaga actgaaccgt gtgttaggcg gaggtgtggt gccagggtct
     3661 atggtcttga ttggtggcga tccaggtatc gggaaatcca ctttgctgtt acaagtttct
     3721 cagcaattag cagccatagg cggaaaagtc ttatatgtat ccggcgaaga aagcgcagag
     3781 cagataaaat tacgcgcgga acgcctcgga agtatcaaca cagaatttta tctatacgct
     3841 gaaacagata tgaacgaaat cagtcgtgcc atcgaacata tctcccctga ttatgttatc
     3901 attgattcca tccaaacgat gacgcagcca gacatcacaa gtgtggcagg aagtgtcagc
     3961 caagtaagag aaaccactgc agaactactc aaaaccgcta aaacaaatgg gatagccatt
     4021 tttatcgtcg gtcatgttac caaagaaggc tcgatcgccg gcccaagaat gttagaacat
     4081 atggtggata cggtgttata ttttgaaggt gaacaacatc atagttttcg gattttgcga
     4141 gcagtaaaaa accgatttgg ctcaacgaat gagattggga tttttgaaat gcatgagcat
     4201 ggattggaag aagtcgctaa tccttcacag attttcttag aagaacgcct agacggtgca
     4261 actggctctg ctatcgttgt ggcaatggaa gggacacgtc caattttggt agagatccaa
     4321 gcattagtca cacctactat gttcggaaat gccaaacgaa caacaacagg tcttgatttc
     4381 aatcgggtat ctctgatcat ggcagtgctg gaaaaaagag ctgggttgct tcttcagaat
     4441 caagatgctt acttaaaagc tgcaggtggg gtaaaattaa atgaaccagc gattgattta
     4501 gctatcgctg tcagtattgc ttcaagctat aaagaaaaag gaacacagcc aacggaatgt
     4561 ttcatcggtg aaattggact gactggagaa atcagacgtg tcagtcatat tgaacaacga
     4621 gtcaaagaag tccaaaaact cggcttcaca aaagtatacc tacctaaaaa caacttaggt
     4681 aactgggaag cgccaaaagg aatcgagata gttggagtag caactttggc tgaaacgttg
     4741 aagcgtgtgt tccgctaact aataaatgat tgtcgaaacg gaggaaaaac gatgcaaaaa
     4801 cgtgtcatta ctttgctgat gatcattgtc ggcgcaagct tgggtatttc atttttaccc
     4861 tcagcttggc aggcaatcgg tcaacagtca aacggatggc tgaataatac atttaccaac
     4921 agcctgttag gcgcacttat tttcttttta ttatctttgc tgttggccaa acatatctca
     4981 gctattatca aacaagtaga acaaaaacta agtgaggtca gcttgactta tcttctcttc
     5041 ggtgcgatcg gtgcgatcat cggattgact ttaggtgttg tgatctcgac ccctctttac
     5101 aatatggaaa ttccattcgt caatagtgta ttgccgattt tactgatgat catcttaggc
     5161 tatctgggat tgagaatggg aacgacacga atcgaagaat ggaagaaagt ttttggttca
     5221 cgaagcaaaa aaatcgagca agaaacagct agtcaagttg agagtcaact gttagaacga
     5281 aaatcaggag aaaatttcca taaatataaa atcttggata caagcgtgat cattgacggt
     5341 cggatttatg atattacaaa gactggattc ttagaaggaa ccttgatgat ccctaatttt
     5401 gttctttacg aactacaata catcgcggac tcaggggata gtttgaaacg tgtgcgtggt
     5461 cgtcgcggat tagatatcct aaatgccttg caaaaagaag agggtatatc tgtcgaaatg
     5521 tacgatggag actttgaaga tatcagtgaa gtagacagca aattgatcaa attggctaag
     5581 cttttagatg gtgtcattgt aaccaatgac tataacttga ataaagtctc tgaattccaa
     5641 aatgtacctg ttcttaatat caatgcgttg gcaaatgccg taaaaccagt cgtcatccct
     5701 ggtgaaacga tgaatgttct agtagtaaaa gccgggacag aacgccaaca aggagtagcc
     5761 taccttgatg acggcacgat ggttgtcgta gaagatggac agcattacat gaatgaacgt
     5821 atcgaagtcg ttgtaacaag tgcccttcaa acagctgctg gacgcatgat ctttgctaaa
     5881 cctgcacatg caggacgagg aatcgacaat aaaaaagaga cagagaaaaa gactaaacaa
     5941 tcaaaataaa aaagaaattc agccaactgg taaggaaatt caaccagttg gctttactat
     6001 ttaccttaga aaaggtaaaa tttgtgctga gtggctttaa ataaatgata tttgagaagc
     6061 cgtaactgac ggaatttttg ctgttagtca aagtctagtt tgaaagaaga ggaagaaaat
     6121 gacgaaagta cgcgtacgct atgcaccaag tccaactggg cacctgcata ttggaaatgc
     6181 aagaactgca ttattcaatt atttgtttgc ccgccacaat gatggagact ttattattcg
     6241 tattgaggat actgaccaaa aacgtaatat cgaagatggc gaaaaaagcc agttggaaaa
     6301 ccttgcttgg ttgggaatga attgggatga atctcctgaa aatcctggag aatacggccc
     6361 ttaccgtcaa tctgaacgta aagaaatcta tcaaccgctg attgatcagc tgcttgctag
     6421 caatcgagca tacaaatgct attgcacaga agaagaatta gaagcagaac gggaagcaca
     6481 acgcgcaaga ggagaaatgc ctcattacgc aggaacatgt gcgaacttga cacctgaaga
     6541 acaagcagaa aaagaagcac aaggtctaga atctgttgta cgtttccgtg tgcctcgcaa
     6601 tacagaatac gcatttacag atatggtgaa aggcgctatt tcgttcgaat ccgataatat
     6661 cggtggcgac tttgtcatcc aaaaacgcga tggtatgcca acttataatt ttgctgttgc
     6721 ggtcgatgat catttgatga aaatcactca cgttttacgt ggagatgacc atattgccaa
     6781 tacaccaaaa caactgatga tctatgaagc atttggctgg acaccaccaa ccttcggaca
     6841 tatgacattg atcatcaaca gtgaaacagg taaaaaacta agcaaacgtg acgaatcaat
     6901 cttgcaattt atcgaacaat atcgcgaatt aggctatcta ccagatgcaa tgttcaactt
     6961 tattgcatta ttgggctggt cacctgtggg cgaagacgaa atcttcgaac accaagaatt
     7021 gatcaaaatg ttcgatccaa aacgcctaag taaatcaccc gcagctttcg atgcgaaaaa
     7081 actgcaatgg gtaaacaacc attacatcaa agcaatggat ctagatgcat ttactgacat
     7141 gtgtctgcct tatctaatcg ctgatggacg tgtagcagaa aacccagatg aaaagacaat
     7201 cgaatgggtg aagaagatcg ttagcctgta tcaacctcaa atgagctatg ctgcagaaat
     7261 cgttgatctg tctacgctat ttttcaatga acatcctgta ttgaatgaag cagcaaaaga
     7321 agtacttgct ggtgaaacag ttcctactgt tctccacgca ttcaaagcac agctagaaca
     7381 aatggaagta gtagatgctc caactgtcaa agctgccatc aaagctgttc aaaaagaaac
     7441 tggcgtaaaa ggaaaaaatc ttttcatgcc aatccgtgta gctgtatcag gacaaatgca
     7501 tggtccagaa ttgcctgaaa cgatcgaatt gcttggtcgt gaaaaatcat tagcacattt
     7561 aaatcaagta ctgaactaaa cacgatgaac agaaaaaagt aagcagctga tcattacaag
     7621 agagataccg ggtggtgcaa ggtatcaatg aatggttgcc gaaatgcctc tgggagtggt
     7681 cttggcgaaa cacagaaaac aggaaaaact gttttatagg aaacaaagta gccaagaacg
     7741 tataacaggc gttagctgtc agagataatc attgattatg aagaaaagtg gaaccacgcg
     7801 gaaagcgtct tttgataagg aaacttattt aaaaggcgct tcttttcttt ataagaagat
     7861 gatttcctac ataaaattct tacgaaagga gtggaaagtc atgagttggt ttacccgagc
     7921 gattgatgca gcaaagagaa acgatccggc tgcacgttcg acagctgaga tcgttttgac
     7981 ataccctggt tttcatgcct tgttttggca tcgtttctct cattttttat atcagcaccg
     8041 gctcttttta ttggcgaaga tcaatgctca attctggcgt ttcctaactg gtgttgagat
     8101 ccatccagga gcaacgattg gaactggcgt gttcatcgac catggaatgg gaatcgtcat
     8161 tggcgaaacc gcagaaatcg aggacgatgt gattttattc catggcgtaa cattaggcgg
     8221 cacaggaaaa gaaacgggta aaagacaccc aactgttaaa caaggagcca tgctctctgc
     8281 gaatgcgcag atattaggac cggtaacgat cggaaaaaac gccaaaatcg gtgcaggtgc
     8341 cgttgtgttg aaagatgttc cagacgacgc tacagcggta ggagtaccag caaaagtcgt
     8401 tcgaatcaaa ggagaaaaag tgaggagatc agaatgataa aaatctacaa tacattgacc
     8461 agaaaaaaag aagaattcat ccctatacaa aagggacatg tcggtatgta tgtctgcgga
     8521 ccgactgtgt ataattatat ccatatcgga aatgctcgta gcaccattgc tttcgataca
     8581 attcgacgct attttgaata tcgaggctat aaagtcgatt tcgtatcgaa tttcacggat
     8641 gtagacgata aaatcatccg tacagcaaac gaattaggta ttacagcacc agaagttgct
     8701 gatcgtttca tccgagcatt tgaagaagat acgaaagcgt tgaatgtcga acctgctaca
     8761 ctgcatccaa gagtcatgga tcatatgcca gatattttag catttattca agtgttggtt
     8821 gaaaaaggct atgcttatga atcttcaggt gatgtgtatt accgtacccg taaatttgaa
     8881 aattatggga aattaagtga ccaatccatc gatgaactgg aagtcggagc aagtcagcgt
     8941 acaggggaag aacagcagca aaaagaagac ccattagatt tcgctttatg gaaacaagca
     9001 aaacctggcg agatttcttg ggattcacca tggggcaaag gacgtcccgg ttggcatatt
     9061 gaatgttccg ttatggcaac aaaacattta ggagaaacga tcgatatcca tggtggcggg
     9121 caagatttag aatttcctca tcatgaaaat gaaatcgcac aaagtgaagc gaaaacaggg
     9181 aaaaaattcg ctaattactg gatgcacaat ggctatgtaa cgattggcga agacgatgaa
     9241 aaaatgagta aatctctagg gaatttcatc actgtacatg aaatgatcaa aaacgttgat
     9301 ccacaagtct tgcgtttctt catggcgacc acacaatacc gtcgtccgat ccgctacagt
     9361 gaaacgacga tgaaagaagc agccaccaat ttacaacgac tgaagaatac gtttgacaat
     9421 ttgaaattcc gccaagaaac tgcctcagag aatttggcag atgatgcgga taaaatcgct
     9481 ttgttagaaa atttagaaca gcgattcatc gaagaaatgg acgatgattt taatgcagca
     9541 aatgggatca cagtggtcta tgagcttgct aagtggctga acacttatct agaccagccg
     9601 aaagtttctg ccgctgtctt ggcacaagga gaagctttat tctcgcaatg gctttcgatc
     9661 ttcggtattt tatttacagc agaagagtta ttagatgaag agattgaaca attgatcgaa
     9721 gaacgaaatc aagcaagaag caatcgtgat tttgtccgaa gcgacgaaat tcgtgatttg
     9781 ttgaaagaaa aagggatctt attagaagat acaccgcaag gaacccgatg gagaagagaa
     9841 gcatgagaga ttacacacaa ttgaacggtt tagccttggc ttatgttgga gatgcgattt
     9901 acgaaatcta tgttcgtgac cacctgattg atcaaggaga aacaaaaccc aataagctgc
     9961 atcgtgcggc aacgaattat gtttctgcga atgcacaagc ctttttgatt cagcaaatgc
    10021 tggaagaaca tcttttgaca gaagaagaag aactttatta tcgcagagga agaaatgcta
    10081 aaagccatac gagcgcaaaa aatgctgatg ttacgacgta tcgggtcgca acagggttcg
    10141 aagcgctcat gggctatctg catttgacaa aacaaaccga gcggatggaa gaattgatcc
    10201 gctggtgtat tcagaaagtt ggtgaaaaaa atgggtaaag aaagaacaaa caaacatcaa
    10261 tcgaaacgtg gatttgaaga caaaaaacaa aatagatatt caaataaaaa gcaaactgga
    10321 cgaaatcagt cagtttctga tcaaaatgaa gaagcattag aagataattt cgtctttggg
    10381 caccatgccg cagtagaagc gctacaacaa gggcgaggga ataaactctt tcttcaagaa
    10441 gattcacgag gagaaaaagt agaagaactg aaagaaatcg cacgtgaaca agctgtgcca
    10501 gtcaaatggg ttcctaaaca aaaattagaa actttgagcg atcacggcgt gcatcaagga
    10561 gtcgttcttg cgattacacc ttatgaatat ttatcccttg accaactgat tgaacgaaca
    10621 aaagagaaaa aagaagaccc cttctttttg attttggata gcatcgaaga tccacacaat
    10681 tttgggtcga ttttacggac agcagacgca agtggcgtcg atggtgtgat cataccaaaa
    10741 caccgagcag ttggaatcac acctatcgta acaaagactt ccactggggc agtcgaacat
    10801 gtccctgttg cacgcgtgac gaatttggta caagctgtgc aacagctcaa agaaacagga
    10861 ttttgggtat ttggcacaga tatgaccggc acaaacttcc aaaaatggaa tgccaaagga
    10921 tcaattgctt tagtcattgg taatgaaggc aaaggaatga gtccaggatt gaaaaaagaa
    10981 atggatgaat tgctgacgat cccgatgacc gggcacgtcc agagcttaaa tgccagtgtt
    11041 gctgctggtt tgcttatgta tcaggctttt tcccaacgtc agggggagta gaccatgaaa
    11101 aaacagctgt tgattgtaga cggctacaac atgattggtt cgtggccgga acttgtccag
    11161 cttaaaaagc aggacaaaat ggcggatgca agagaagctc ttttacatcg tttatcaaat
    11221 tatgcgaaat atgaaggaat ggaagtcatc gttgtcttcg atgcccaact agttccgggc
    11281 atccaacaaa attataagaa ataccagttg gatgttgttt ttacgaaaga agacgaaacg
    11341 gctgacagtt atatcgagag aatcgctggt cagctgaatg accgtttgac acaagtgaca
    11401 gtggcgacaa gcgatttagc agaacagtgg atgatctttt cccaaggagc attacgaact
    11461 tctgcttatg aattatataa gacgataaaa aaaacggaaa aaacaatagc ccaagaaaat
    11521 aaagatatgc attttattga tttccgacgt aattctccat ggaatgaaga gcagttaaaa
    11581 aaattgtccc aaaaattgga cgatttaatg agaaaaaata aataacatat cgttctaatc
    11641 agttcgatga atcattgatg ataagattcc tccataaacg aatttatgga ggaattattt
    11701 tatgtctgag gagttattgc gcgctgcacg taatggtgac gaagaagcat ttgctgccct
    11761 gtatcaaaaa tattttccac ttttactacg tttgaaaaac acctattatt taagaaatta
    11821 tgtttctgag gattgggaac aagaagcaag gatcgcttta tatcaggcat tgagaactta
    11881 cgaaaaaagc aaaggtgtta gctttggaag ttattataaa agcgttataa gcaatcaaat
    11941 ctattctctg ttgaggaagc agaacgcgct gaagcgtcgt gatcaaaaag atgaagtctc
    12001 aatcgaccaa aaaatagaga cagaaggacc agacttttta gcagagatca cattgcaaga
    12061 acctttagca attcagcatc ttttattaaa agaagcaatc gaaaattgcg aaatccagtt
    12121 ttcaaaaata gaagctaaaa tttttcatta ttatatgcaa ggcgttgatt ttgtagacat
    12181 ggctgaatgt ttagcaatgg atcacaagca agtaatgagc gcttacggga gagtaaaac
//
LOCUS       DAJSBC010000077        11802 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSBC010000077 DAJSBC010000000
VERSION     DAJSBC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.078"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..194)
                     /locus_tag="ONT65_002278"
     CDS             complement(<1..194)
                     /locus_tag="ONT65_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329875.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFN"
     gene            complement(210..824)
                     /locus_tag="ONT65_002279"
     CDS             complement(210..824)
                     /locus_tag="ONT65_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR2329876.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(971..1654)
                     /locus_tag="ONT65_002280"
     CDS             complement(971..1654)
                     /locus_tag="ONT65_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2329877.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1763..2221)
                     /locus_tag="ONT65_002281"
     CDS             complement(1763..2221)
                     /locus_tag="ONT65_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR2329878.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2305..4068)
                     /locus_tag="ONT65_002282"
     CDS             complement(2305..4068)
                     /locus_tag="ONT65_002282"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR2329879.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4289..5749)
                     /locus_tag="ONT65_002283"
     CDS             complement(4289..5749)
                     /locus_tag="ONT65_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329880.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5957..6883)
                     /gene="glsA"
                     /locus_tag="ONT65_002284"
     CDS             complement(5957..6883)
                     /gene="glsA"
                     /locus_tag="ONT65_002284"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR2329881.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6906..8333)
                     /locus_tag="ONT65_002285"
     CDS             complement(6906..8333)
                     /locus_tag="ONT65_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2329882.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8364..9578)
                     /locus_tag="ONT65_002286"
     CDS             complement(8364..9578)
                     /locus_tag="ONT65_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR2329883.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9919..10161)
                     /locus_tag="ONT65_002287"
     CDS             complement(9919..10161)
                     /locus_tag="ONT65_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2329884.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10193..10750)
                     /locus_tag="ONT65_002288"
     CDS             complement(10193..10750)
                     /locus_tag="ONT65_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2329885.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10763..10951)
                     /locus_tag="ONT65_002289"
     CDS             complement(10763..10951)
                     /locus_tag="ONT65_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2329886.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10964..11530)
                     /gene="amaP"
                     /locus_tag="ONT65_002290"
     CDS             complement(10964..11530)
                     /gene="amaP"
                     /locus_tag="ONT65_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2329887.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3461 a         2447 c         1932 g         3962 t
ORIGIN      
        1 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
       61 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
      121 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
      181 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
      241 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
      301 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
      361 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
      421 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
      481 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
      541 tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
      601 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
      661 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
      721 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
      781 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
      841 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
      901 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
      961 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     1021 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     1081 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     1141 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     1201 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     1261 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     1321 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     1381 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     1441 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     1501 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     1561 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     1621 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     1681 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     1741 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     1801 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     1861 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     1921 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     1981 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     2041 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     2101 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     2161 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     2221 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     2281 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     2341 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     2401 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     2461 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     2521 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     2581 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     2641 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     2701 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     2761 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     2821 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     2881 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     2941 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     3001 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     3061 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     3121 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     3181 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     3241 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     3301 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     3361 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     3421 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     3481 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     3541 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     3601 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     3661 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     3721 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     3781 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     3841 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     3901 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     3961 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     4021 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     4081 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     4141 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
     4201 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
     4261 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
     4321 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
     4381 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
     4441 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
     4501 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
     4561 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
     4621 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
     4681 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
     4741 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
     4801 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
     4861 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
     4921 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
     4981 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
     5041 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
     5101 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
     5161 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
     5221 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
     5281 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
     5341 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
     5401 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
     5461 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
     5521 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
     5581 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
     5641 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
     5701 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
     5761 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
     5821 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
     5881 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
     5941 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
     6001 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
     6061 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
     6121 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
     6181 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
     6241 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
     6301 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
     6361 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
     6421 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
     6481 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
     6541 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
     6601 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
     6661 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
     6721 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
     6781 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
     6841 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
     6901 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
     6961 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
     7021 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
     7081 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
     7141 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
     7201 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
     7261 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
     7321 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
     7381 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
     7441 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
     7501 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
     7561 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
     7621 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
     7681 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
     7741 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
     7801 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
     7861 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
     7921 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
     7981 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
     8041 tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
     8101 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
     8161 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
     8221 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
     8281 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
     8341 cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
     8401 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
     8461 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
     8521 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
     8581 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
     8641 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
     8701 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
     8761 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
     8821 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
     8881 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
     8941 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
     9001 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
     9061 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
     9121 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
     9181 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
     9241 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
     9301 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
     9361 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
     9421 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
     9481 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
     9541 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
     9601 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
     9661 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
     9721 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
     9781 tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat ataaaatata
     9841 taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca tttcaacata
     9901 agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta atactaaaat
     9961 gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc cccaagttcc
    10021 tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg cgatgatccc
    10081 cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac cgccgacgat
    10141 taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact tcttattgta
    10201 cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca gattttgctt
    10261 tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt actttgtctt
    10321 gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca acattcacat
    10381 tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc aattctttga
    10441 tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct agatccacag
    10501 ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct gtgttgacta
    10561 atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca tctacatctt
    10621 tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat tcaccattca
    10681 tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta ttcatgtttt
    10741 tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt cctgactgtt
    10801 tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac aatgtcttga
    10861 aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca aagataatag
    10921 ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc actcgagagt
    10981 gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact gccactttga
    11041 ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc atcaatacac
    11101 ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg atatggattc
    11161 gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct tcgtgtaaat
    11221 gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca tcttcccgtt
    11281 tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag atgataatag
    11341 caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag taattcgtta
    11401 aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca ttgatcaacg
    11461 caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt atttttaacc
    11521 ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac tctaccgaca
    11581 atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc taccaaacat
    11641 tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat tgcttactta
    11701 ttctctattt acttttgatt gctggctata cttaaaggat attattcaag attaaaaatt
    11761 gcaaagatta tgctcagtta ttattacaag attctattta tt
//
LOCUS       DAJSBC010000078        11314 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSBC010000078 DAJSBC010000000
VERSION     DAJSBC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.079"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1251)
                     /locus_tag="ONT65_002291"
     CDS             complement(<1..1251)
                     /locus_tag="ONT65_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2329888.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
     gene            1579..2058
                     /locus_tag="ONT65_002292"
     CDS             1579..2058
                     /locus_tag="ONT65_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329889.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            2091..4319
                     /locus_tag="ONT65_002293"
     CDS             2091..4319
                     /locus_tag="ONT65_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2329890.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4448..6091
                     /locus_tag="ONT65_002294"
     CDS             4448..6091
                     /locus_tag="ONT65_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2329891.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(6164..6709)
                     /locus_tag="ONT65_002295"
     CDS             complement(6164..6709)
                     /locus_tag="ONT65_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR2329892.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6732..7217)
                     /locus_tag="ONT65_002296"
     CDS             complement(6732..7217)
                     /locus_tag="ONT65_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2329893.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(7207..8019)
                     /locus_tag="ONT65_002297"
     CDS             complement(7207..8019)
                     /locus_tag="ONT65_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR2329894.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(8158..9690)
                     /locus_tag="ONT65_002298"
     CDS             complement(8158..9690)
                     /locus_tag="ONT65_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR2329895.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(9799..10830)
                     /locus_tag="ONT65_002299"
     CDS             complement(9799..10830)
                     /locus_tag="ONT65_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2329896.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3714 a         2099 c         2186 g         3315 t
ORIGIN      
        1 tttgaaattg taaaagttgc gaaagccgta cgcattgcgt tttaatactt tgatgtggtt
       61 gtttaagcat tccaatggtc cgtttgaata gctgtagtga agcgcatttt ggatctgtgg
      121 cagaaacttt ttaaacgttc gtaaagtggt tgtataggtt tctggcagac cgctaatgtc
      181 tctagttaaa caatcctcaa atagtgactg atctcttttc tttaccgcat aaagaatgtc
      241 ttggtataac tgatagcctt ggcgtaagtt gggacaagca gctaacagtc gatcggcaat
      301 ctcactttca gtcaaataat ctttaaaact ggcacgccaa acccgttttt catagtttaa
      361 ctttgcttct tctttttgta ggagtttcca atagcgcttc aattttttcc ctaaccctcg
      421 ttgagagtgg gaatttcctt taagtcgtac cgtggtttct ttcactcgat gattcaagaa
      481 agttctgcca atatgttgga cgatgtggaa acgatcgatg atcaactgag cattcgggaa
      541 aagctttttc accaacgaaa cataaggcgc atacatatca ataacaatca attgaacggc
      601 ttcacgagtc gctaatgaaa accgagaaaa atagcgttct aaataaggca agcgtcgatt
      661 ttcgacaata tccaataatg catggctttg tccgtccatc atgataaaac tcattgcact
      721 ggcaacactt cgaaccgatt tgaattcatc aaagcataag acctttggca aagtctgttt
      781 gaaaggattc aatgggcggt agaatcgctt catgacacga tagaccgaag aagtggaaat
      841 atttttctga cgagcgattt ccgtcatgga agtattctct tttaagcgag caatgacaga
      901 ccaacgaact ttttgactaa tgaaacagtg tttttcagca acagaggtat ccgctacgaa
      961 tgttctttgg caagacttac atttaaatcg ttgtttcttc agctccagat aagtgcgata
     1021 ctcagacaca tcattcagta acaaccgaac ggttgtcgtc ccccaacgaa tgatcctaga
     1081 ggggacgaca caaagacaat gggaacactc ttctggcttg tacagaagag ttcctttgta
     1141 aatcaagcaa atttgccctt tgtatttttc tttagttaaa caattttcct cgaaatagat
     1201 atttaggtct agaatattta gggtatcttt gataagctga gtatgagaca tcttgaatcc
     1261 tcctatgaat tggtagccaa attaattcta aaggatttca gggtgtcttt tctatatttt
     1321 tttatacaaa aaagatgttg gtgaagttca ccaacatcaa aaagtataga gccaaaatat
     1381 tccaacatca tcttggactt tctacatgtt actaatgagc gtgtaaaaga acgagatggt
     1441 gtttttattt ctatcaatga agctcttttt ctttgcaatc ctcacttcaa tcctctataa
     1501 tgtcgcatat agtttagtaa atgagtaaac tactaattta cttatttact aaaaataaaa
     1561 ggatgaggag ttaaaataat ggaaatacca acaaatttaa aacataaacc agtaatcgtt
     1621 gtagaagact atgataaagt tgatggaaga aatgcattaa atatggatgc aaaagggctt
     1681 tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg caaggtatgg
     1741 cgacatactg gagaaaaatg gtcacgacag tcggaagaat taccaatcac acgtatttta
     1801 gatttagcga tcttagctgc gcaaggaagt ttatacttcc aagatgctta tcgttatgaa
     1861 aaattttatg atccagaaaa tccagtcgta gacatcattg gattacaagg gaaccgaatg
     1921 acggtagaag tagataccaa aaatccaaag atcgatgaag acatcatttt gtattacgat
     1981 acactacaaa aagacggaga actgattggg gaacgattcc gtattttaaa acgtctttta
     2041 gatgagctag gatattaata gacagagatg atcaaaaggg gaagagaagc atggagaatt
     2101 tctcactaac agaaaaccga accttttcac ggctagaaaa gcaaatgatc tggaagaaac
     2161 cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc aaagggaatt
     2221 ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga aggagatgcc gggtcgggga
     2281 agacacaatt agccaaagca ctatctgctg atctgcaact tccctataca aaagtgacct
     2341 gttttgcaga tatggacaaa tcagatgttt tcggagcctt attacctgta gtagattcta
     2401 ataaggaaca agaccaagaa ttactagaag ctatttacca aacagataca cttgaagctg
     2461 tactgacact gatccagaac cattacggca ttgatccttt ttctgctaaa gaaaaattag
     2521 cagagctagt tcaaaggatc gaagctgatt atccagatgc aatccattac agctattacc
     2581 cttctgaaat cgttcgagcg attgaaaaag gctatttatt agagattcaa gagccgactg
     2641 tcatccgaga tgcctctgtt cttgtggcac ttaactcagc gctcgaacca aatgggatgt
     2701 tgaatttacc gacaggaatc attcgtcgcc atccagattg cgtcattgtg atcactacaa
     2761 atcgaaatta ccaaggaaat cgcccattaa atgaatcttt aagagatcgg atgcaacatg
     2821 cggaaaaaat ggatcttcct gccttagaag tcatggcaga acgtgcgatg gcgaaaacta
     2881 tgatccaaaa acaagaactt ctcttaaaga tggcagagat tatccgtttg ttggatgaaa
     2941 ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg ttcttatttt tattgggcaa
     3001 atacctataa gcaaggacaa gatttactcc agtcgattta tccaaaagtt ttgtataaat
     3061 tatccactga ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac agtggattgc
     3121 tactggaact tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa tctgagcata
     3181 caaaagaaag aaccattagc gccgaagaag caaatgaacg aaacatcact gaaaatgctg
     3241 aagaaattct gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca aatgagggag
     3301 aagagaaaca agaagaaaca atagaaaaaa acgagattcc tcctcaaatg caaaaagaac
     3361 gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca aacaggagat gattctcaag
     3421 aaatgtctgc ttctgacatg gaagcacatg ataaggaaat caagaagcaa ctgaataaag
     3481 aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga aggtctcatc gttcaccgac
     3541 cagctttcca aggaaaaacg ccggaagcac gtgagctaca catgcaagta atgccaatag
     3601 tagaatcact tagccggcaa attttagagt tactggataa tgaacaaaca gagacgtacc
     3661 aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa agtagcatat ggtgatttga
     3721 gaaattttga caaaaagaac ccgccacatg agcaaccttc tttagcggta gctgtacgga
     3781 tcgatgaatc tgggtcaatg atacgggatg atcggatcga agcggcaaaa aaagcagcga
     3841 ttgccattgc cgaattttct aagaaagtcg atatcccatt gttgatttac ggagatacag
     3901 cagaccgttc gtcaagagaa aagacttctt tgttttctta taaagaattt gaagacggat
     3961 ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat cgtgatggtg
     4021 ctgcacttcg tttagcagca gaaaaattaa acagacaatc cgcaacaacc aaactgttgc
     4081 ttaatattag tgacggtcag ccaaaagcgt tgccggatta tacaggcaaa aaagcaaaag
     4141 aagatatcca gtctgttttg aaagaatatg aacgtcaagg aatcctattt gtctccgcag
     4201 ctattggcca agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata
     4261 ttactgactt gaatgaattt cccaagcaaa tgattcaact gattgcccgc tatctatgat
     4321 aaaataaagc tcagcgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg
     4381 gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg
     4441 aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc
     4501 tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa
     4561 acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc
     4621 tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg
     4681 aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat
     4741 tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa
     4801 atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga
     4861 accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg
     4921 acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa
     4981 attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga tagatggatt
     5041 tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa
     5101 ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca
     5161 tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga
     5221 aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat
     5281 ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga aacatgcatt
     5341 aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca
     5401 tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc
     5461 tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg
     5521 acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga
     5581 ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga
     5641 tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca
     5701 atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat
     5761 caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat
     5821 ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga
     5881 acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt
     5941 aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac
     6001 attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta
     6061 tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga
     6121 ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag
     6181 gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt
     6241 tcaaacgagg tctgacaggt acttggacat gcttgatatg cataccgatt gatgtgtcac
     6301 cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt
     6361 caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt
     6421 cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac
     6481 aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga
     6541 tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg
     6601 tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat
     6661 taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc
     6721 ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga
     6781 aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca
     6841 gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt
     6901 cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca
     6961 tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc
     7021 aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga
     7081 aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca
     7141 gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt
     7201 aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc
     7261 ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc
     7321 tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa
     7381 ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata
     7441 atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt
     7501 gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt
     7561 taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat
     7621 catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac
     7681 agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt
     7741 ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat
     7801 accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga
     7861 tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct
     7921 tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc
     7981 aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa
     8041 gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca
     8101 atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta
     8161 ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt
     8221 tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac
     8281 ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg
     8341 tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa
     8401 taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc
     8461 tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc
     8521 gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt
     8581 agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt
     8641 aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt
     8701 caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac
     8761 ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt
     8821 gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc
     8881 cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg
     8941 gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg
     9001 aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat
     9061 gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt
     9121 ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga
     9181 agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc
     9241 aggttgttcc attaaataaa gaataggaat ctgttttgat tttgttccat ttgaaatttc
     9301 tctcactcgc ttttttgtct catcatcaaa taaaatgaaa gggtcttcta aacgatcgat
     9361 aaatacttcc attgttgttg ttggtagatg ataagaagtc atgacaggca ctgcaccaag
     9421 gtaagacaca gaaacagcta gaaggtatgt gtcaaaagcg gaactcttat aaataataac
     9481 tttgtctccg gatttcactc caagatgagc caattgataa gcccgtttca acacactttc
     9541 gcagcatttt ttgtaagttg tttcaagtcc taattctggg aaagctgaaa gtggctcatc
     9601 aaagataatc ggggtctcgg aaaagttagc tgcagcttct tgaaaattcg taaataagtt
     9661 caaaggggaa tacgttatgg tctccatcat tcacactcca ttatcttttg ttaagtaata
     9721 tacaaattca agataatcgt aacataatcc tttatataat aacatagttt attgtgaaaa
     9781 aaagatcata catttgtttt atatggaaga aacccgaacg aatgccgcta attcgtgata
     9841 agaaaaatca accatctttt cccagatatc ttgaaaagaa tgggatgaga ggtatcgatt
     9901 accacataaa taatttaccg cgtcagcatc atctaaatat agttctgcat aataacgata
     9961 tgtttttctg catagttctg gcgatacgtt tttatatact gtaattttgc tcaaaatttg
    10021 gcgaatagtc cgaggagaaa tttgctgatc atgtgaggaa cgaaaaacaa aagaattttc
    10081 gtccgtattt tgtgctgtta acttaaaata gttctccaga aatggcatcg cttcttttgg
    10141 tattggtact ttattcagtc tgttttcttt tctattcaga ttaagaatct gcttggtcaa
    10201 agatacatct ttacgtttaa gttctactac ttcatggatc tttaatccgc aatacagaag
    10261 aatggcagta attgcaaggt tcctcggtct attcttttct tcaaagctag cttctctgct
    10321 tgtttgaaag ccttcaatcg ggataaacaa atgctcaaaa tctttgaaaa gttctgcatc
    10381 gatatatcgc ggagctgctt tggttgtttc cggtaaactt gtacccaaaa gagaaatctc
    10441 ttcttcaatc agtccttttt gatacaaaaa tcgtaaaaaa tgatttaatg aatattgctt
    10501 ccttgaaatc ccgtaatagc tattttcaaa caattgctcc gtttcttggt tcgctgtagt
    10561 ttggaacgaa cgagtatgtc gatagatttt agctaagaaa ttctgataat cttgtttttc
    10621 taaatggata atcagctgat catccacttc ttcagaaaat acttttctca aataatcagt
    10681 aaacagatga aaatcttttg cgtattcata gcgactgcct aatgaaaggg aagaacgatt
    10741 ttcttctaaa aactgttgaa agacggaagg gagcaagtgt gtacgttgct ttaacttttc
    10801 ttgcagttgt tttctagttt tgctgtccat tacaaattcc ctccaatagt gttcattttc
    10861 tatattttac cgcatttcat caaatgaaga tagacagttt gtcttgatct taggaagcaa
    10921 atttttaact aagtactagc taaaagatat gaagtaagaa gatacatttt tagttatttt
    10981 attagtgcag tcattctttt gatcttttaa ttgatttatc ctctttttag ctatgtggtt
    11041 acttcatgag caatgaaatg gatttggttc gttctaatgg caggaggctc tttcttttat
    11101 ttccgaaaaa ttagaaaaca ttttttcaga atcgattggc tctccattta ttcaataatg
    11161 gtttgtctaa cgatattgtt gggataattc ttaaagaagt attagaaata gttggtactt
    11221 cattacttta catgaagatt tattctatgg taggagcaat tatattgaga attaatcctt
    11281 ttttatataa gttgtttgct tattgatgga ttgg
//
LOCUS       DAJSBC010000079        11128 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSBC010000079 DAJSBC010000000
VERSION     DAJSBC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.080"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="ONT65_002300"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="ONT65_002300"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR2329897.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="ONT65_002301"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="ONT65_002301"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR2329898.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="ONT65_002302"
     CDS             2139..3020
                     /locus_tag="ONT65_002302"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR2329899.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="ONT65_002303"
     CDS             3017..3733
                     /locus_tag="ONT65_002303"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR2329900.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="ONT65_002304"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="ONT65_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR2329901.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="ONT65_002305"
     CDS             4450..5451
                     /locus_tag="ONT65_002305"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR2329902.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="ONT65_002306"
     CDS             5448..6809
                     /locus_tag="ONT65_002306"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR2329903.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="ONT65_002307"
     CDS             6769..7920
                     /locus_tag="ONT65_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR2329904.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="ONT65_002308"
     CDS             7895..9064
                     /locus_tag="ONT65_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2329905.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="ONT65_002309"
     CDS             9239..10618
                     /locus_tag="ONT65_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2329906.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="ONT65_002310"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="ONT65_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3701 a         1946 c         2495 g         2986 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcatacagtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgcaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAJSBC010000080        10928 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSBC010000080 DAJSBC010000000
VERSION     DAJSBC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.081"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..288)
                     /locus_tag="ONT65_002311"
     CDS             complement(<1..288)
                     /locus_tag="ONT65_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2329907.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="ONT65_002312"
     CDS             430..1548
                     /locus_tag="ONT65_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2329908.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="ONT65_002313"
     CDS             1676..3052
                     /locus_tag="ONT65_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2329909.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="ONT65_002314"
     CDS             3453..5033
                     /locus_tag="ONT65_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR2329910.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="ONT65_002315"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="ONT65_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="ONT65_002316"
     CDS             complement(5659..5979)
                     /locus_tag="ONT65_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2329911.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="ONT65_002317"
     CDS             complement(6127..8361)
                     /locus_tag="ONT65_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2329912.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="ONT65_002318"
     CDS             8840..9106
                     /locus_tag="ONT65_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR2329913.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="ONT65_002319"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="ONT65_002319"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR2329914.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3478 a         1912 c         2216 g         3322 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaa
//
LOCUS       DAJSBC010000081        10624 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSBC010000081 DAJSBC010000000
VERSION     DAJSBC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.082"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            21..644
                     /locus_tag="ONT65_002320"
     CDS             21..644
                     /locus_tag="ONT65_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329915.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1094..2143
                     /locus_tag="ONT65_002321"
     CDS             1094..2143
                     /locus_tag="ONT65_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2329916.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2140..3021
                     /locus_tag="ONT65_002322"
     CDS             2140..3021
                     /locus_tag="ONT65_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329917.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3024..3794
                     /locus_tag="ONT65_002323"
     CDS             3024..3794
                     /locus_tag="ONT65_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2329918.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4073..4927
                     /gene="aroE"
                     /locus_tag="ONT65_002324"
     CDS             4073..4927
                     /gene="aroE"
                     /locus_tag="ONT65_002324"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR2329919.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5105..6268
                     /locus_tag="ONT65_002325"
     CDS             5105..6268
                     /locus_tag="ONT65_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2329920.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6385..6795
                     /locus_tag="ONT65_002326"
     CDS             6385..6795
                     /locus_tag="ONT65_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2329921.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6969..9197
                     /gene="topB"
                     /locus_tag="ONT65_002327"
     CDS             6969..9197
                     /gene="topB"
                     /locus_tag="ONT65_002327"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2329922.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9368..9652
                     /locus_tag="ONT65_002328"
     CDS             9368..9652
                     /locus_tag="ONT65_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329923.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9812..10048
                     /locus_tag="ONT65_002329"
     CDS             9812..10048
                     /locus_tag="ONT65_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2329924.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10045..10443
                     /locus_tag="ONT65_002330"
     CDS             10045..10443
                     /locus_tag="ONT65_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2329925.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3711 a         1771 c         2247 g         2895 t
ORIGIN      
        1 gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag
       61 aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc
      121 ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa
      181 aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga
      241 aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga
      301 tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg
      361 agcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg
      421 aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag
      481 gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt
      541 tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg
      601 atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga
      661 aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa
      721 aataacaaat aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta
      781 aatcaattct taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa
      841 aacaaaaaaa attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat
      901 agatacaact ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga
      961 ctgccgcata gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca
     1021 ttgtcttttt ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg
     1081 caggaggaaa aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta
     1141 tttagcaggt gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa
     1201 ccagacaaaa aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga
     1261 tgagatttat cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa
     1321 tcttgagatc aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca
     1381 acagctagta caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc
     1441 attagcaaac acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg
     1501 ggctggatta gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca
     1561 gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt
     1621 cggaatcttg tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga
     1681 agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat
     1741 tgcacaaacg gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa
     1801 taccattgcc aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt
     1861 cattccatct gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca
     1921 atatcaatta gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa
     1981 accggaaacg acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca
     2041 agctgatcgc ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac
     2101 agatgactcg cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca
     2161 caaggcatgc tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac
     2221 tttcctgata tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct
     2281 attactcatg gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata
     2341 gcaggactcg ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg
     2401 attttagtga tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt
     2461 tctttattga atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac
     2521 tttgacacag tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc
     2581 ttcttcaata cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct
     2641 cggtcgttag ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt
     2701 gtcattgcat tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa
     2761 gggattggcg taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa
     2821 gaattgacgc tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta
     2881 ttgattttag tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct
     2941 atcatcttag ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg
     3001 ggttttgaga aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag
     3061 tcgtaaacaa tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca
     3121 gagaagggga cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca
     3181 atacgatcgc tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga
     3241 tcacaaaaga ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca
     3301 agatgggaac ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag
     3361 gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac
     3421 tatgtcaaga agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat
     3481 ctggtggtca acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac
     3541 tattattaga tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat
     3601 taacaaatca gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg
     3661 aagacgcctt gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag
     3721 atatttctga aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag
     3781 aagtagtgga ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt
     3841 gacatcttcc tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg
     3901 ttttgtagga tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac
     3961 aagtaaacga tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt
     4021 atcaaacgct aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc
     4081 tggacaaacc aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt
     4141 gatgcacaat actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt
     4201 aaatccaatg aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt
     4261 aaatctttcg atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga
     4321 agcgcaattg atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta
     4381 taataccgat ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa
     4441 gaaaaaaatg actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagctgc
     4501 gttagacggc gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt
     4561 aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt
     4621 tgccgatacg catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac
     4681 agatatcggg atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca
     4741 tgagaatcta gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga
     4801 agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc
     4861 aatcgcgttt gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt
     4921 tgattaaaaa aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa
     4981 gtaatccttt atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg
     5041 ataggctttt gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac
     5101 cacaatgagt cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg
     5161 ggatacgggg agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga
     5221 agatgctacc cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga
     5281 gcaacagata tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt
     5341 caatcggcaa aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat
     5401 cattctatcg attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa
     5461 atatgtagct gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca
     5521 aaaagcgacg aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga
     5581 tgccgggcga gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa
     5641 actgatttct tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat
     5701 caattcagct tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt
     5761 tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga
     5821 tgccttattc atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt
     5881 agatattaaa aaaggggtcc agaagatgga tggacatgtt cttttgcagt acgcacgttt
     5941 ccgaatggat gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc
     6001 tattttcagc caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa
     6061 agctttagga tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc
     6121 gattgcacga ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa
     6181 ttatgggcaa agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc
     6241 tgctattaca gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag
     6301 gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt
     6361 taaccaagag aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt
     6421 gaagccatcg ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac
     6481 ggtgaaatgc ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg
     6541 aatgcacggc tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt
     6601 ggtcctgaat tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa
     6661 gaaacgaagc gcctatttga aagcttagct gagggtggta agattgagat gccactggat
     6721 gctgttccgt gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag
     6781 attaaccaag agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga
     6841 tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta
     6901 ttgccagatg cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag
     6961 gtcagaagat gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg
     7021 aaaccttcca acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg
     7081 ctggtgaagt aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag
     7141 cttatgaaga gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct
     7201 tccgttacga agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc
     7261 agtcagccga taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt
     7321 ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga
     7381 tcaattctct tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga
     7441 actattttcc aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa
     7501 tgaatggaag tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt
     7561 ctcttggtcg tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca
     7621 aacacttcaa aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc
     7681 agttttctgg aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg
     7741 aaatagaaaa acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga
     7801 aaaaggaaaa gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga
     7861 tgaatcagct aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg
     7921 aaagcaagtt tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc
     7981 agtatctggt taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga
     8041 caactgagat caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg
     8101 caattattcc gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac
     8161 agcaagcaat ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata
     8221 tctacgaaga gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa
     8281 agatcccatt acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag
     8341 ctcagactct tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg
     8401 agaaagaaac acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa
     8461 ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa
     8521 tagggacaga agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg
     8581 aagtcaataa aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag
     8641 ccaatgaacc attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa
     8701 aaattggtaa acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc
     8761 tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta
     8821 gcgggacacg tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa
     8881 aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat
     8941 gcgacttcaa attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag
     9001 atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga
     9061 aattcgatgc agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga
     9121 tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg
     9181 aaaacgaagt agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag
     9241 aatataaata ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg
     9301 ctgattttat atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa
     9361 ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga
     9421 ttacgggacg aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat
     9481 tttatcgact ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag
     9541 catgctaaag cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat
     9601 tatttatttt ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga
     9661 gagtttgtga caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa
     9721 gcaaaataaa agtatataca ttgattaaaa gtatatactt ggatataatg tacttactat
     9781 agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag
     9841 aaaatctggc aatagcgatg taacaactat cccaccagaa gtaaaagaat tcatgggtgt
     9901 gcagacaggt gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa
     9961 agcacaagaa gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga
    10021 cgcgttaaaa gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc
    10081 aacgcgaaag ttattttacg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat
    10141 gcattagata tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatcct
    10201 gaaatctatg aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat
    10261 aatggcaata aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt
    10321 ccagttgctt tttctcgtca agaagctgtt gattttatca taatgattac cactagttca
    10381 ttggatttcg attcactaaa gtccaagata accagttatt tacgacaaac cgcaataaaa
    10441 tgaacgaatt aaaaacttta tttctcaatt agatcagcat gtcattaagg aatatgctga
    10501 tttttttatg tttaatcata caaaatagtt ttttcataaa aatatggaaa atatggatta
    10561 attttgaaat aattgattta aaaggaatac aaaagagtga atagtaggaa atagtggttt
    10621 agaa
//
LOCUS       DAJSBC010000082        10585 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSBC010000082 DAJSBC010000000
VERSION     DAJSBC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.083"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            190..339
                     /locus_tag="ONT65_002331"
     CDS             190..339
                     /locus_tag="ONT65_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR2329926.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            350..1864
                     /gene="dltA"
                     /locus_tag="ONT65_002332"
     CDS             350..1864
                     /gene="dltA"
                     /locus_tag="ONT65_002332"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR2329927.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1861..3066
                     /gene="dltB"
                     /locus_tag="ONT65_002333"
     CDS             1861..3066
                     /gene="dltB"
                     /locus_tag="ONT65_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR2329928.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3111..3344
                     /gene="dltC"
                     /locus_tag="ONT65_002334"
     CDS             3111..3344
                     /gene="dltC"
                     /locus_tag="ONT65_002334"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR2329929.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3347..4615
                     /gene="dltD"
                     /locus_tag="ONT65_002335"
     CDS             3347..4615
                     /gene="dltD"
                     /locus_tag="ONT65_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR2329930.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4756..5838
                     /locus_tag="ONT65_002336"
     CDS             4756..5838
                     /locus_tag="ONT65_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR2329931.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5838..6641
                     /locus_tag="ONT65_002337"
     CDS             5838..6641
                     /locus_tag="ONT65_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2329932.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6711..7451
                     /locus_tag="ONT65_002338"
     CDS             6711..7451
                     /locus_tag="ONT65_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2329933.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7462..8466
                     /locus_tag="ONT65_002339"
     CDS             7462..8466
                     /locus_tag="ONT65_002339"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR2329934.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8511..10355)
                     /locus_tag="ONT65_002340"
     CDS             complement(8511..10355)
                     /locus_tag="ONT65_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329935.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3440 a         1722 c         2122 g         3301 t
ORIGIN      
        1 tctttatttt ttatattttt taaaagtacg agaaattata aagaaaaaag cagtgatttg
       61 atgtgctatt tttataaaag ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa
      121 gctagacaca tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt tatatgagga
      181 gggaatccaa tgaacaaatg gcgttctaat ccaaaattga attattgggg aaaattcatc
      241 attcgaacaa ttttttatag tgcagtactg ctgttcctga tttatctgta tcactataaa
      301 aatattcaag gaggaacttt tatctataac gaattttagg aggaagagta tggaaatcaa
      361 aacgattatt gaagcaatcg atgattgggg aataaatgaa cctgatcgag ttgcttatca
      421 ggcggatgag acgcatactt ttggtgaatt gaaacaagca tcagacgcac ttgcttatta
      481 tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt tttggcaatt tggagtttga
      541 aatgattgtc tcatttctag gggttgtcaa atctggccat gcctatttgc cgattgaaga
      601 gcatacaccc aaggagcgta tcctatcgat ctttcgggtg gctaaaccat ctatggtcat
      661 cagtatcggg gactggatag aagatattcc ttcggttcct gtcataacaa aaaaagaatt
      721 tcaagaaatc aagaagatac cagtaggatt ttatcccaga aatagcgtaa aaggtacaga
      781 aaatttttat attattttta cttctggtac tactggagaa ccaaaaggcg tacaaatcag
      841 tcatgataat ctagtcagct ttgttcaatg gacgatggaa gactttggga tcaagcctgg
      901 tatgcatttc ttagcccaag cgccattttc ttttgattta tcggtatttt ctatttatcc
      961 tgcattagtt tctggcggaa tgctgaagcc gttaaataaa gcagttgttc aagactttcg
     1021 tcaattattc gcgacattgc cagccttgaa gttgaatgtg tgggtttcta ctccatcatt
     1081 tatggatatt tgcttgatgg aacctacatt caatggagaa aatgtgccgg aattggacat
     1141 gtttttattt tgcggagaag aattgacgaa aaaaactgca gaaagtctac ttgatcgatt
     1201 cccaaatgca cggatatata atacttacgg tccaacagaa gcaactgtag ctatttcaag
     1261 tattcagatt gaccagcatg tactagatag ttatgaccga ctaccgattg gctacgtaaa
     1321 aaaagacacg aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg
     1381 tgaaatcatt attgcaggac caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac
     1441 agctgctgcg ttcttttgtc aaaatgactt agcaagttac catacagggg atgcaggtag
     1501 actagatgaa tatgggcttt tattttatga aggacgtatg gatcagcagg tcaaattaca
     1561 tggataccgg attgaactcg gggatatcga acattatctg ctccaagaca accgaatcaa
     1621 acaagctgtc gtcgtaccaa aataccaagg aacaaaagta cagcagctgg ttgcttttgt
     1681 cgtattagaa aacaaaacga aacagccgga ttttcagttg actaaatcta tcaaagaaca
     1741 attactagaa gttgtaatgg attatatgat tcctcagaag ttccattatg tcgatctgct
     1801 gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta acagctgagg tgaatcctga
     1861 atgatgttac ctcatatgat cccttatgca gagccgtttt atttttttct tttagccatt
     1921 gcgcttgtac cgatcgttct atctttattg atatgggaaa aaagattacc agtttatcaa
     1981 tcagcagtga cacttttctt tttatttgtc agctttggag gtaattattg gcaacaggga
     2041 gcggcgctga ttgggtatgt tttgtggcag acgatacttg tatggggcta ttttgtttat
     2101 cggcaaagga aaaatcagac aggatggttt tttgccgccg ttattttagc gataattcca
     2161 ctgtttttaa cgaaagtgac tccttttttt gaacatggaa aagcatcgtt acttggtttt
     2221 ttaggaatct cttatttaac ctttaaatct gtacaaatga tcatggaaat aagagatggc
     2281 atgatcaaaa catataagcc atttcgttat atccagtttc tgctgttctt tccaacaatc
     2341 tcttctggac caatcgatcg ctataggaga tttgaaaaag atcagctcca tccgcctgtt
     2401 cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa atatttttgt cggtttttta
     2461 tataaattca ttatcggtta ttattttggt caagtactgc ttcctgtagt gggacgcatt
     2521 gccttacagc atggtggtat ttcatgggca ttagtaggtt atatgtacgt gtacagtatg
     2581 tatttgtttt ttgattttgc tggctatagc ttatttgcag tcggtacaag ctacatgatg
     2641 ggatatcaga caccaatcaa tttcaataaa ccttttttat catggaatat caaagaattc
     2701 tggaatcgtt ggcatatgac gctgtccttt tggttccgtg attatgtata tatgcgattg
     2761 atgttcttca tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt
     2821 tattttgctt tgtttcttct aatgggtata tggcatggat tgacgtggta ctacatcgta
     2881 tatggactgt atcatgcttg tctgatctgt ctgacagatg catggatccg atttaagaag
     2941 aagcataaag agcagttgcc ttctaacaaa tttacgcatg gcttagccgt atttttgaca
     3001 tttcaagcag tatgttttag cttcctgatt ttttcaggat ttttagataa actttttttt
     3061 agctaacgaa tagtgacata ttaatttata taacaaagga gaaaaggaaa atggaagaac
     3121 aaattttatc aatcttagaa gaaataacag gaacagatga agtaagaaaa aatcgtgata
     3181 ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc
     3241 tagacggaga gctggatatc caagtccctg tatctgaatt cgagcgagag gattggagca
     3301 caccgaataa aatcattcaa caagcaactg cattgaaggg ttgagtatga aaaagaaact
     3361 ttttgctatt ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt tttttacctc
     3421 ctcctatcaa gtagatatct ctaatccaga aacgattaga cgtgcctctt cttccatgtc
     3481 agtgaacgtg ttgaaaggag atgcgataaa aaattatgca ttatctgaaa aacaatatat
     3541 tccttttttt ggctcaagtg aattaagcag aatcagtcca tttcatccgt ctgttctggc
     3601 agaaaaatac cagcgaaact atcgtccatt tttgctggga gcacccggga cacaatcgtt
     3661 aagccaatat atgatgatgc gttctgcagg agatgctatg aaaaacaaga aagtcgtctt
     3721 tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa actgattact ttaatacgta
     3781 ttattctgaa cttcaaacat acgactggtt gttttcaatg aagaaagtaa cgcctgctga
     3841 tcgctattta gctagaagat tattaacttt ctcaaaagta aaagagaatg atacgttgac
     3901 ggcgattctg caaacgatca aaaaagggaa gctaccttta cctgaatctt tgaatcagct
     3961 gcgcagccaa tggaacatgc tgaagagaga ggatgaagta ttcagcaata ttgggttgaa
     4021 agatcgacag caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat accaagaaac
     4081 agaattatcc attttagcaa atcaaattgg agaacgagaa acgacgaata atccattcgg
     4141 gttgaaaaac gatttttata cgcatcgtat ccgagcccac gagccagagc tcaaacagtc
     4201 acaaaaaaat tgggattatc gtttttcgcc agaattttct gattttcagc tggtcttgga
     4261 tcaattagca aaaaaccata acgaagtatt gttcatcatc ccaccggtta acgagaaatg
     4321 gagcgattat accggactat ctcaagaaat gttacaagga tttgccaaaa agatcaaatt
     4381 tcaattaaat agccaaggat tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa
     4441 ctattttatg gaagacacga tccatctagg ctggaaaggc tggttggctg cagatcaaca
     4501 gatcagaccg tttttagaag aaaatcatat tactgctagt aagtatcatc ttgacgatgc
     4561 tttttttagc aaatcttggc aacatcaaat accagataaa ctacaattaa aataaagaaa
     4621 acaggcgagt gttgctcgct tgttttcttt atttttgaat attctgaata aaagataaaa
     4681 ataccaagtt tgcctgaaca aaaatcataa gcaatggtat aatcacaata tgaatataga
     4741 aatgggggaa aacatttgga tacaaaagaa gaacagctac ttattgattt aacttctgca
     4801 aaaggagtac ctggaaatga agaagaggta agagaagtat tcagagaata tgccaaacca
     4861 ttcgctgatg atatttttta tgacggttta ggcagtgtga ttgccaaaca tggtgctggc
     4921 ggaggcccca aggtctttat ttctggacat atggacgaag ttggttttat ggtaacaaaa
     4981 ataaccgaaa aaggattctt ggaattccaa actcttggag gttggtgggg gcaagtcatg
     5041 cttgctcagc aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg tgtgattggt
     5101 tccaaaccac cacatgtttt aacacctcaa gtaagaaata aaccttatga aataaaggat
     5161 atgttcattg atattggcgc atcaagccaa gaagaagcca aagagtgggg catacgtcct
     5221 ggagatatgg tcactccata tattgaatac aaacgaatga atggcagtaa atatttactg
     5281 gcaaaagcat gggataatcg tatcgggaca gctgtttctc tgcgagtatt agagaatcta
     5341 tctaaagaag cacacccaaa tgttttattc gcaggaagcg atgtgcaaga agaagtaggc
     5401 ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg atattgcttt tgctttagat
     5461 actggtacag caggagacac tccaggaatg acaccaaaag aagcagattc gattttagga
     5521 aaaggcccac agattttgat ttttgatgca tctatgatcc cgcataaaaa attattgaat
     5581 tttgttattg atgtagcaga ggaaatggat attccattcc aatatacagt gatttctggc
     5641 ggaggaactg atgcaggaca aatgcatttg acaagagatg gtgttccgtc tgtagccatt
     5701 accgttcctg ttcgttatct gcattcccat acatcagtga ttcatgaaga tgattatttg
     5761 aatacagtga aactagtcac agaattagtg aaacggcttg atagtgaaac agttgaaaaa
     5821 attcggagtt atgaataatg aataatagaa tacctatttc tgaatatcgg aaaaaagcac
     5881 gagtttcttt ggaaaatcaa tggggaatca atacttggct tgttttctta tctataatat
     5941 ttacgagcgt catccagaac ttttttgacg gtttcagtcc ttttccggaa gaaagcatgc
     6001 agcagcactt attgagtttt ctagtcgata atttactttt attcgcattc aactatgcat
     6061 tgttttacat tgcgctagta gttgtacggg gtggcagacc aaaaagtaac cttttatttt
     6121 cagttttcca aagaaagtat tttggttcga tacttggcat caatttgatc aattcagtca
     6181 tcaactggct actcaacagt ttaatctttc tgcccatgtt tttaatcagc ggagtgagta
     6241 cttatatgca gctgcttttc agttcaaatg cggtacctac gggtgtcatg ccagaagatt
     6301 ttcttgatat cagctttgtt ttatcgatga tagggatgat ttttgtttca gcactcatca
     6361 tgagtattgt tggcggttta ttccaatttg ctgcatggac aaagttcgac tatcctgatt
     6421 tgtcggttat tcaatgtttg aaatatggct ggtttttatt gaaagataga attggtacgt
     6481 atattttgtt gcagctgtct tttatcggct ggtatttgct aggagcaatc gctttgttca
     6541 tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt tatgaacaag
     6601 cacggattga aaaaggaggt ccagacgcct attttactta gcatgtaagc agtttattca
     6661 tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg ggaatgtacg atgaaattaa
     6721 ctgttttagg atgcttagga gcgtatcctt ataaaaatca agggacaaca ggttatttgc
     6781 ttcaatctga cgggttcaat ttgttgatcg atgcaggtag tgctacgctg atcaaattac
     6841 aagaacacct tgatccactt gatctagatg ctgtgatcct tagccattat catcatgatc
     6901 atattgctga ccttggtgta ttacagtatt actggcaact tcatcctaag agaaatccgg
     6961 agacattgct ttcaatttat ggacacacaa aagattccat tcactttgaa gaattgacgt
     7021 tggaaaatgt ctcaaaagga catccttatt ttgaagctga agaattaaaa attggtcctt
     7081 ttctgattac ttttatggaa acaatccatc cagtagtatg ttacgccatg cgtatcgtag
     7141 aagaaacaac ggggaaagta ttcgtattta caggtgattc aggttatttg gaaagtttcg
     7201 ctgattttgc gaaagatgct gatttgtttc tagcagatac ctatcttttt gaaggaaatg
     7261 aacatcatca tgcccatttc acttcaaaag aagctggaga acttgcgaaa tcagctaacg
     7321 taaagaaact tgtacttaca cacctacctc agtttggtga tttagaacaa ttgaaaaatg
     7381 aagcgcaaaa ggcagcaggc aaagggattg atatagaact tgctgcagtt gacaaagtgt
     7441 ttaacattta ggaggaagaa catgattttt gtaccaaatg aaaaccacga cccaagaatc
     7501 aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc tattttgctc
     7561 ttttatatca atgaaccttc aatcatcatc ggcagaaacc aaaatacgct tgaagaaatc
     7621 aatcgagatt atgtggaaga aaatggcatt catgttgtcc gtcgcttaag tggaggcgga
     7681 gctgtctacc atgattttgg aaacctgaac tttagtttta tcatgcctga tgatggggat
     7741 tcattccgtg actttgcgaa agtaacacag ccaatcatcc aagcacttca tgaattgggt
     7801 gttgctgggg cggaattgaa aggccgaaat gatctagtca ttgatggcat gaaattttct
     7861 ggcaacgcga tgtatgctac caacggtcgg atgtttgctc atggaacaat tatgttcgac
     7921 tctgatatca atgaagtagt caatgcatta aaagtaaaaa aagacaaaat cgaatcaaaa
     7981 ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac catttcttcc agaagaaaag
     8041 caaaaaatga caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt cggcgtagag
     8101 tcagtagaag aagtaaaaac gtacgaattg actgatgaag attggaaaaa aatcaatcga
     8161 atctctgagg aatattatcg aaattgggat tggaattacg ggaagtctcc agatttcaat
     8221 ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag tccatttgaa tgtctcagaa
     8281 ggaatgatca aagaagcaaa aatctttggc gatttcttcg gactaggaga tatctctgac
     8341 gtggaaaaaa aattggttgg acagaaatat gataaagcat ctctatcaaa aatagtcagc
     8401 caaattgatg tgaagaaata ttttggtgcg atagaaccag aagaattgct taccttactt
     8461 tattgaaaat aaaaaatagc ggaatccaca tatatggatt ccgctatttt ttatacagga
     8521 ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc
     8581 gttagtgttc ctgtgtaacg atccgctacg tctttcatca tcattaagtc aaaacgttcc
     8641 tgatcatcta attcttctgt gatgcctcga ctaaataggt ttgtatcgta atgtgcacta
     8701 tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta tcgacaaata aatttttcga
     8761 tacagtggat tttcgatggc atgagtttct ctaaaggaat gctctaaagc gtggatcaaa
     8821 agttgagcca gatcgtcatc gttgatagga agttcttcgg gaagatctgc ttggaaggtt
     8881 atctgaatat tccgtttggc agctaacttc tggtaacgcg ctaagataag atttgtcacc
     8941 tggtggttgc aaaaagaaca cagcatttcg aaattttttg cttcttctaa tatcttttcc
     9001 acatagtttt tagcttttgt ttggtccgta tgcattaaat cttgcagatt ctccatctct
     9061 tggacaaatt ccaggcgaag tgtatttact tcttggatat gctgtcgaag ctgctcataa
     9121 tgttgctcca acaaatcatt tttgattttt aagaaatcta cttgtctccg atctttgtcg
     9181 gtatttaaga tgtgatcgat tagattgtag ctgataataa cgattagaat gataatatat
     9241 aaaatcgtag accaattgat cgttgtttga tagataccta atgccgtttg gatgtaaatc
     9301 aagcaatgga taatggctac caagacgatc caaggtgtac aagctacata aaaacggtta
     9361 ttcttatatg cttcgccaat gcatgcaagt gaagtgacta aagtgcttat aacactagca
     9421 atattagcaa tcggcattaa ttcaggaagt tctgcaggag taaaagttgc ataaagcaga
     9481 acgccgataa aaatcaaaag ttggaacgta caaaaaattc cgtaccattt tttatagtaa
     9541 aacattcggc tccaatagta gcagatgatg aaagctgacg ttaagataat aaataaatga
     9601 gataaaaaag aatgaacgaa tggttcaaaa agaataccac ttaaaatatc ttctgataca
     9661 gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa taagtgagta ttcaagacgt
     9721 ttttgtttat acatcaagta gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg
     9781 attatcatcg tcaatacttg cggcactgat tgtgaataaa taaaactaat caatggtcca
     9841 gttgttccta gataaacttt cggtggcagt ccagcatagt tctgataagg agatgagact
     9901 gtaagggtca atttttttcc gatataattt gctggtaaat ctatcacagc caatgagagt
     9961 ccgggattgc tttcttcctg ataatctttc cgttggtata aaatcttttc gcctagatat
    10021 acagtaatcc attgatggtt tcctttgatt agtaaatttg cttcgtccaa cggtttttca
    10081 agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg taggaatcgt acgagtgtac
    10141 ttgctgtcaa acttaaaatc aggatcatct tttgtataga aaacccacga atcttttatt
    10201 tctttcattt gattgctgtc cgcaggagaa aaaataaaat aaaagaaata agcaattcct
    10261 gttaaaaata acaatgataa tataatgatt atgctccact ttatacgtaa ataaaaagat
    10321 tcttttttat tatatatgtt attaaaaaaa gacataaagg tattcctttc taaaacttgt
    10381 aatgttaggg taaacgtttt ttaaactaac gtcaattatt tataataaaa aatgagataa
    10441 agatgaggaa atagaattag aaacatataa ttattaaagg gaaacatttt tacagctata
    10501 atttgaaaaa aatttatcgt tattatatga ttaagaagga ttgaataaag aaagaagttg
    10561 aaaacgatac ctgattacaa aaaaa
//
LOCUS       DAJSBC010000083        10356 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSBC010000083 DAJSBC010000000
VERSION     DAJSBC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.084"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="ONT65_002341"
     CDS             6..305
                     /locus_tag="ONT65_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2329936.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ONT65_002342"
     CDS             378..1739
                     /locus_tag="ONT65_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR2329937.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ONT65_002343"
     CDS             1761..2021
                     /locus_tag="ONT65_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329938.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ONT65_002344"
     CDS             2035..2787
                     /locus_tag="ONT65_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR2329939.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ONT65_002345"
     CDS             complement(2881..3486)
                     /locus_tag="ONT65_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2329940.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ONT65_002346"
     CDS             complement(3539..4513)
                     /locus_tag="ONT65_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR2329941.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ONT65_002347"
     CDS             complement(4891..5610)
                     /locus_tag="ONT65_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2329942.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT65_002348"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT65_002348"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2329943.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ONT65_002349"
     CDS             7161..8558
                     /locus_tag="ONT65_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2329944.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ONT65_002350"
     CDS             8639..9079
                     /locus_tag="ONT65_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2329945.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ONT65_002351"
     CDS             9227..9553
                     /locus_tag="ONT65_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2329946.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ONT65_002352"
     CDS             9554..10303
                     /locus_tag="ONT65_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329947.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3286 a         1826 c         2146 g         3098 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagact
//
LOCUS       DAJSBC010000084        10086 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSBC010000084 DAJSBC010000000
VERSION     DAJSBC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.085"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(247..759)
                     /locus_tag="ONT65_002353"
     CDS             complement(247..759)
                     /locus_tag="ONT65_002353"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR2329948.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT65_002354"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT65_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR2329949.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ONT65_002355"
     CDS             complement(3212..3655)
                     /locus_tag="ONT65_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR2329950.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ONT65_002356"
     CDS             complement(3656..4444)
                     /locus_tag="ONT65_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2329951.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="ONT65_002357"
     CDS             complement(4441..5382)
                     /locus_tag="ONT65_002357"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR2329952.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ONT65_002358"
     CDS             complement(5559..6146)
                     /locus_tag="ONT65_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR2329953.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT65_002359"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT65_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR2329954.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ONT65_002360"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ONT65_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2329955.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ONT65_002361"
     CDS             complement(9063..9998)
                     /locus_tag="ONT65_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2329956.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAJSBC010000085         9515 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSBC010000085 DAJSBC010000000
VERSION     DAJSBC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.087"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..152
                     /locus_tag="ONT65_002362"
     CDS             <1..152
                     /locus_tag="ONT65_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2329957.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ONT65_002363"
     CDS             complement(230..763)
                     /locus_tag="ONT65_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR2329958.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT65_002364"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT65_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR2329959.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT65_002365"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT65_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR2329960.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT65_002366"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT65_002366"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2329961.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ONT65_002367"
     CDS             complement(4488..5066)
                     /locus_tag="ONT65_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR2329962.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ONT65_002368"
     CDS             5388..5858
                     /locus_tag="ONT65_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR2329963.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ONT65_002369"
     CDS             complement(5966..7192)
                     /locus_tag="ONT65_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2329964.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ONT65_002370"
     CDS             complement(7946..8146)
                     /locus_tag="ONT65_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2329965.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ONT65_002371"
     CDS             complement(8280..8888)
                     /locus_tag="ONT65_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR2329966.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ONT65_002372"
     CDS             complement(8973..9443)
                     /locus_tag="ONT65_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR2329967.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         2825 a         1908 c         1564 g         3218 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaa
//
LOCUS       DAJSBC010000086         9425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSBC010000086 DAJSBC010000000
VERSION     DAJSBC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.088"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            94..1248
                     /locus_tag="ONT65_002373"
     CDS             94..1248
                     /locus_tag="ONT65_002373"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR2329968.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ONT65_002374"
     CDS             complement(1270..2022)
                     /locus_tag="ONT65_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2329969.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ONT65_002375"
     CDS             complement(2386..5052)
                     /locus_tag="ONT65_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2329970.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ONT65_002376"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ONT65_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2329971.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ONT65_002377"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ONT65_002377"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR2329972.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8282..8557
                     /locus_tag="ONT65_002378"
     CDS             8282..8557
                     /locus_tag="ONT65_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329973.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            8984..>9425
                     /locus_tag="ONT65_002379"
     CDS             8984..>9425
                     /locus_tag="ONT65_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2329974.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFL"
BASE COUNT         2842 a         1909 c         1641 g         3033 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8221 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8281 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8341 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8401 cacagaaaaa ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat
     8461 gtttactgaa aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa aagaatttcc
     8521 aagaagtcat aaactctatg tgaatcagca attttaactt ctaactgttc tttattaatt
     8581 aaaaatgttc tgtaataaaa taaaacttta taacaactat aacaaaaccg aatgcaaaac
     8641 agaagttcta taagttataa tcatagttgt taggtgattc atctagtaaa aacaccagat
     8701 ttcaaatttc acacaattta aactagatca atcatcaacc ttcttcaaat tgttagaacc
     8761 ctttcccgag gagctgcagc ataagcaaaa aaacaaacag gaagccaaat ggctaagaca
     8821 ttttttctat tagtctagtt gtttctcgtc gcaagtttaa gactttagaa agttatttct
     8881 aatgatgttt gggttgtttt gatcaatgct tatgcacagc tccttaccta ttatttatct
     8941 caacagatct aacaaattaa tggactattt aaggagtttt tttatgctta cgatctattc
     9001 ttctcgcaca catagttacc atctgatggc aaactggctg aaagaccatc aaatgcctta
     9061 cagagaacga attttcacac ctaaacagcc tttaacaagg aaagagatat tttatttatt
     9121 gtcattaaca gaaaacggat ttgacgatct attagctaaa cgctcgattg attataaaaa
     9181 tttgaaagac caaatcgacc aatgtacgac ctcagagctt gtcgatctga tcgtctcgca
     9241 ttttactttg ttacaacgtc ccatcattac tgacggaaag aaattattag tcggattcaa
     9301 tgaagatagc atgcgtcttt ttatccctgt acaatggcgc agaagtcaac tgaaatctgt
     9361 ctataacgat tttacatggg taagcaatct agaaaattct acggatttag atgatgaatt
     9421 tttaa
//
LOCUS       DAJSBC010000087         9361 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSBC010000087 DAJSBC010000000
VERSION     DAJSBC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.089"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(92..760)
                     /locus_tag="ONT65_002380"
     CDS             complement(92..760)
                     /locus_tag="ONT65_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2329975.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="ONT65_002381"
     CDS             894..1181
                     /locus_tag="ONT65_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329976.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="ONT65_002382"
     CDS             1597..3135
                     /locus_tag="ONT65_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR2329977.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="ONT65_002383"
     CDS             3132..4097
                     /locus_tag="ONT65_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329978.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="ONT65_002384"
     CDS             4084..5406
                     /locus_tag="ONT65_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2329979.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="ONT65_002385"
     CDS             5647..6390
                     /locus_tag="ONT65_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2329980.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="ONT65_002386"
     CDS             6405..7397
                     /locus_tag="ONT65_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2329981.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="ONT65_002387"
     CDS             7409..8146
                     /locus_tag="ONT65_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2329982.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="ONT65_002388"
     CDS             8136..8858
                     /locus_tag="ONT65_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2329983.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="ONT65_002389"
     CDS             complement(8942..>9361)
                     /locus_tag="ONT65_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR2329984.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DAJSBC010000088         9306 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSBC010000088 DAJSBC010000000
VERSION     DAJSBC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.090"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(154..318)
                     /locus_tag="ONT65_002390"
     CDS             complement(154..318)
                     /locus_tag="ONT65_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2329985.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            613..1941
                     /locus_tag="ONT65_002391"
     CDS             613..1941
                     /locus_tag="ONT65_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR2329986.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2143..2976
                     /locus_tag="ONT65_002392"
     CDS             2143..2976
                     /locus_tag="ONT65_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR2329987.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2992..3729
                     /locus_tag="ONT65_002393"
     CDS             2992..3729
                     /locus_tag="ONT65_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR2329988.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3884..4810)
                     /locus_tag="ONT65_002394"
     CDS             complement(3884..4810)
                     /locus_tag="ONT65_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR2329989.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5085..5918
                     /locus_tag="ONT65_002395"
     CDS             5085..5918
                     /locus_tag="ONT65_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR2329990.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5952..6791
                     /locus_tag="ONT65_002396"
     CDS             5952..6791
                     /locus_tag="ONT65_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2329991.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6817..7377
                     /locus_tag="ONT65_002397"
     CDS             6817..7377
                     /locus_tag="ONT65_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2329992.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7576..9297
                     /locus_tag="ONT65_002398"
     CDS             7576..9297
                     /locus_tag="ONT65_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR2329993.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
BASE COUNT         3165 a         1565 c         1914 g         2662 t
ORIGIN      
        1 agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt
       61 ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa
      121 tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa
      181 tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat
      241 gacattccca aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc
      301 agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc
      361 aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat
      421 acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc
      481 gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca
      541 tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg
      601 aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt
      661 caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct
      721 tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct
      781 acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac
      841 acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat
      901 tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca
      961 acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa
     1021 gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa
     1081 tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac
     1141 aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta
     1201 gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc
     1261 tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat
     1321 catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta
     1381 gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat
     1441 acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat
     1501 atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt
     1561 ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat
     1621 tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt
     1681 ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa
     1741 tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt
     1801 gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta
     1861 ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat
     1921 ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa
     1981 gagctggctt aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc
     2041 tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata
     2101 tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt
     2161 ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag
     2221 acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa
     2281 aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta
     2341 ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc
     2401 gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa
     2461 acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat
     2521 tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct
     2581 ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa
     2641 gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac
     2701 ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct
     2761 gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga
     2821 ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga
     2881 ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac
     2941 ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa
     3001 aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca
     3061 tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga
     3121 agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg
     3181 gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag
     3241 tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta
     3301 gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt
     3361 aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa
     3421 aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc
     3481 gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga
     3541 tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta
     3601 gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa
     3661 acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa
     3721 aataaataaa aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta
     3781 cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt
     3841 gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg
     3901 aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt
     3961 tttgactatt aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc
     4021 cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag
     4081 ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg
     4141 cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt
     4201 ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca gcggaaatgt
     4261 ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc
     4321 cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca
     4381 cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt
     4441 cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt
     4501 cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat
     4561 caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata
     4621 tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga
     4681 tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg
     4741 tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt
     4801 tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttt cattttacag
     4861 acttccttac gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt
     4921 tttgctatca gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta
     4981 aaatggaaaa gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt
     5041 tacaagaaag cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat
     5101 taacatcgtt taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat
     5161 atgcagggtt tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt
     5221 cctcagttga gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct
     5281 atcaaggaat cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga
     5341 ttgcgcattt tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt
     5401 tagtccaatt cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac
     5461 ttagaaagtg gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca
     5521 gcgatgaatt tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca
     5581 tcatcgatga gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc
     5641 cgaactttgc tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata
     5701 aagagtggcg taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag
     5761 cacatgaagt caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata
     5821 attctggtgg tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc
     5881 cagatggact caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag
     5941 gggcgaagtc tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc
     6001 gttcaaaagc gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt
     6061 tatgcggtgt agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg
     6121 aattagatat gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta
     6181 tcgaccaccc ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc
     6241 atcgacaaat cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag
     6301 gacaatcgat ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc
     6361 tgatgaaaaa aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt
     6421 tagagatatt aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga
     6481 aaaagttaga acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag
     6541 atatcatccc aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg
     6601 aaattgaaat ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga
     6661 aaggtgtagg aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag
     6721 actttgtgac tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc
     6781 tgatttatta gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa
     6841 aaagtagaaa cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt
     6901 acagaaaatg aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt
     6961 gcagctactt cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca
     7021 ttagataaag cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt
     7081 gaacaagtag atgcactgac agatatcctt tactttactt atggttcttt ttcattacta
     7141 ggaatagtgc ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt
     7201 ccagatggaa aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg
     7261 cagatggaat atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa
     7321 gcgattgcta agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca
     7381 gagtttcact atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt
     7441 agtgaatgca tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg
     7501 tattatttgg atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa
     7561 acggaggaac cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata
     7621 gtcgccattg tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg
     7681 gatgccttag aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat
     7741 gatgttaaaa aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa
     7801 aaatggaatg acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg
     7861 gaaaacctca atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa
     7921 gctacaatgg accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg
     7981 cgggaaacgg aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa
     8041 ctgaaagaaa aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa
     8101 aaacagatca aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga
     8161 gaccctgttg aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa
     8221 gctctgatga aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa
     8281 cttgaagaaa tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa
     8341 gaagatcttg ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat
     8401 cttgaaaaaa cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt
     8461 gattcgttat atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac
     8521 caatcaacga ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt
     8581 gagctggatc acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga
     8641 ggtttccaaa ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc
     8701 cgtcaacacg aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc
     8761 ttagaagata tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa
     8821 ggcgaaaaag aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa
     8881 cgttttgtag aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt
     8941 gttgctactg accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg
     9001 gaagaggtca atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag
     9061 acacatgatt tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt
     9121 tatcgtcata cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat
     9181 aaagaatatc actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa
     9241 cctggtgctt tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc
     9301 aaaaaa
//
LOCUS       DAJSBC010000089         9281 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSBC010000089 DAJSBC010000000
VERSION     DAJSBC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.091"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..435
                     /locus_tag="ONT65_002399"
     CDS             <1..435
                     /locus_tag="ONT65_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR2329994.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ONT65_002400"
     tRNA            complement(759..842)
                     /locus_tag="ONT65_002400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ONT65_002401"
     CDS             1370..1870
                     /locus_tag="ONT65_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2329995.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ONT65_002402"
     CDS             complement(2060..2404)
                     /locus_tag="ONT65_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR2329996.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ONT65_002403"
     CDS             complement(2558..3478)
                     /locus_tag="ONT65_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2329997.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ONT65_002404"
     CDS             3665..4156
                     /locus_tag="ONT65_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR2329998.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ONT65_002405"
     CDS             complement(4180..6555)
                     /locus_tag="ONT65_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR2329999.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ONT65_002406"
     CDS             6881..7507
                     /locus_tag="ONT65_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2330000.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ONT65_002407"
     CDS             7530..8234
                     /locus_tag="ONT65_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2330001.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ONT65_002408"
     CDS             8426..9259
                     /locus_tag="ONT65_002408"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR2330002.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DAJSBC010000090         9048 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSBC010000090 DAJSBC010000000
VERSION     DAJSBC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.092"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ONT65_002409"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ONT65_002409"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2330003.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ONT65_002410"
     CDS             2714..2938
                     /locus_tag="ONT65_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330004.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="ONT65_002411"
     CDS             2951..3451
                     /locus_tag="ONT65_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2330005.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="ONT65_002412"
     CDS             3548..5395
                     /locus_tag="ONT65_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR2330006.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="ONT65_002413"
     CDS             5398..6294
                     /locus_tag="ONT65_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2330007.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="ONT65_002414"
     CDS             6344..6733
                     /locus_tag="ONT65_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2330008.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9048
                     /locus_tag="ONT65_002415"
     CDS             6720..>9048
                     /locus_tag="ONT65_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2330009.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3196 a         1362 c         1909 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DAJSBC010000091         8988 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSBC010000091 DAJSBC010000000
VERSION     DAJSBC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.093"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(130..651)
                     /locus_tag="ONT65_002416"
     CDS             complement(130..651)
                     /locus_tag="ONT65_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2330010.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(653..1687)
                     /gene="sppA"
                     /locus_tag="ONT65_002417"
     CDS             complement(653..1687)
                     /gene="sppA"
                     /locus_tag="ONT65_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2330011.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1720..3135)
                     /locus_tag="ONT65_002418"
     CDS             complement(1720..3135)
                     /locus_tag="ONT65_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR2330012.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3104..5257)
                     /locus_tag="ONT65_002419"
     CDS             complement(3104..5257)
                     /locus_tag="ONT65_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCR2330013.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5515..5751
                     /locus_tag="ONT65_002420"
     CDS             5515..5751
                     /locus_tag="ONT65_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR2330014.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5770..5985
                     /locus_tag="ONT65_002421"
     CDS             5770..5985
                     /locus_tag="ONT65_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCR2330015.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(6688..7440)
                     /locus_tag="ONT65_002422"
     CDS             complement(6688..7440)
                     /locus_tag="ONT65_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2330016.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(7455..8702)
                     /locus_tag="ONT65_002423"
     CDS             complement(7455..8702)
                     /locus_tag="ONT65_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2330017.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(8778..8924)
                     /locus_tag="ONT65_002424"
     CDS             complement(8778..8924)
                     /locus_tag="ONT65_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCR2330018.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         2914 a         1588 c         1269 g         3217 t
ORIGIN      
        1 gttctgtagt atttaaagta gttgaatcaa tagaagatag aaacatacca cccatcaatg
       61 ctaaaagact acataaaatc cctgttattc cactaattaa aatcaaaata aactctgttt
      121 ttctcttatt catttaattc tctccagatt aagtgtttta gaattagaaa ttttttgacc
      181 atagttaata gcttctaatg aacgaataac aaccactgac gtgttagaaa aataatcccc
      241 aatatgttgt ttcttgggcg tgaaagcagc caccaaatac ccaatttgaa atacaaaatt
      301 ttttaatata aacctacata tacactcacg aataaagata gtttgccaac ttagctcttt
      361 ttcctcaaag ctcactactc ttatcccaaa aatcattttc ccaattgttt gtcctgaatt
      421 cagcttagtc aaaagtacaa aataggttaa ataaatagct aatgacgcta taccataaaa
      481 agtaataatg gatttgtcag attccagtcc catgattttg taaaagagac ctatagtagc
      541 atgggtgatg atgctaatac aaattaaatc aataagaaaa gcaaaaaatc gtaaccaaaa
      601 tcccgcaaat acataattag gcaaagtttc aatgccactc ttcttattca ttttatagcc
      661 ctccatagta atacattggt tttggagtat cagaaaatcc aagtttttca aaaactgaaa
      721 ttacacgatc tgatgtagtt gcctttagcc cttgccattc agcaatctta ttacctaacc
      781 aagaagaagc aaagccagtt gtgtcatttt tatattctat aaccgtcgca tcagttaatt
      841 tcttctcttt ttttagggaa tctaatgcat cttcagagta accaatttga tccaccaacc
      901 cattttctac tgcttggtta ccatcataaa ttcttccatc cgcaagttct ttcactgcat
      961 tagtatccat atttcttcct tttgctacaa cattgacaaa acgatcataa gcactcatca
     1021 caaattcctg tataacttta ttctcttcct ctgaaggttt gtgttgaggt gacatcatat
     1081 cttttaatgc accactttta tacgtcgcat cagttacgcc aagtttttct aataagccag
     1141 aataatttaa accagaaata atgacaccaa ttgatccagt tgttgtttct tccgtagcaa
     1201 atattttatc tgaaccagca gaaatgtagt accctccact tgccgctgta tttttaaatg
     1261 cagtatagat aggaatatct agctttttaa tttttgccat ttctttagca atctcagcac
     1321 tttcatatat tcctccccca ggtgaattta cttctaataa cactccctta accgctttgt
     1381 cttcttgaat tttctttagc tgtgttaaaa aattctgatg gttatattgt tccctactaa
     1441 aaagattgct ttcacctgta tctgcaataa ctccattgac ggatagtttg acaatctttt
     1501 tatttgatgc gccttcttct aacgtttctt ctgtgatttc gttagatccg taaaaaatct
     1561 tatttattcc tgttagggtt gtatttgatg cttcctcttt atcttttcct ggaattagcg
     1621 cgacgataat ggatagtaac aataatccgc acgctactcc cacagcaatc catcttcttt
     1681 tattcatata aactcctcct atattcttag taaaaataat tattttttat ttagaaacac
     1741 atcgacaatt tgttgccaat atgttttttc tcctacaatt aaagacaact ctccagttaa
     1801 tccataacga aacggaaacg attgagttgt ttttaacgtt cctttcactg cataaaaaac
     1861 acctttctca gtggcagtag aattttctga aatttcatct aaagttccgt cgatcgtaat
     1921 cggagcccca cctttttgat ccaatttaaa atgaaccttc atcccttctt taatataggt
     1981 actttcattc gcaggtaaaa gtgctgtaaa tttcattttt gatttttcgt ggatcggata
     2041 aatttctgca agcactgttc ctttggctag ttctgcttgt ccctttgttt cttcatttaa
     2101 atgaaccgtt ccagatacag gagcttttag tattcctttt gaaagttgat cgtccaaact
     2161 tttgataact gactggttct tctcttgttt ttcatttagc tctgtaactg tttgttttac
     2221 agttgttatt gcttgttcta gctcttgctt tcctttttcc gtttgactaa ttctgtcatt
     2281 ctcagaagta gttggtttaa ctaatttagc tttttctgac tgatactgtt ctacttcttt
     2341 ttttagttgc tcaatttgtt catttatgct tgctgaaatt gtcagtttca cttgattttt
     2401 ttgatcatca gaaacattat ttagttgttc ttgccaattc tcatagtttg ccatcatttc
     2461 ttttgaaaaa tcttttaatg attgattatt actccaagct acctgtactt gttgccattc
     2521 ttgtaattta ctatctgaac tctcaatttg tttttctagt tgtctttttg tcttttcgta
     2581 tacttctaat tgcttttgat catttaattc actttgcttc aaagcgtagc ggagtgattc
     2641 tttttctgac aacatgctct ttaattgatt actgtagcca aaactgtcat ccgttgaaaa
     2701 caaatttttt cctttactta tactttcaat aaatagctga gccatcttta tttgttgagt
     2761 aatacgttgg ttttcttgtt ctatctgagc cttttcattc tctattaaag aacaatccaa
     2821 tctaaccaac acatcgtcct tatgaacaaa ttgattttct tttaggaagt tttccattat
     2881 tttagcttct aatggcactt gtaacttctc tattttttcg gggttcaatt gagccgttgt
     2941 ccgaataaca acctcttttt tagcaaaggt taaaaacaat ccaataatac ataaaaaaag
     3001 aattacaggg tataacaccc aacggtagaa gctatgatgt tgtttagtat agatactaga
     3061 atgttcgaac caattattat tctgcataaa ttgtttccct ctcctagatt tcccataatt
     3121 tttgatagat accatcggca aaacgaagtt catcgtgcgt tcctttttct accaaatgcc
     3181 cttcatggag aactaaaatt tgatcacatg cttttgctat tggtaaatga tgcgcaataa
     3241 aaataatcgt tttttctttt aaattgagga gataattcat aattgcatgt tctaacaagg
     3301 tatctaatcc actagttgct tcatctaaaa ttaatatgtc cggatttttt aataaagccc
     3361 gtgctaaggc taatcgttgt cgttgctctc ctgaaagatt atttccacct tcttccaaca
     3421 atgtctcgta acgtaatggc tgttgattaa taaattcatt tagttggact atctcacaaa
     3481 cttctattat ttgttcgaaa cttggtgggc tatctaaacc gaatataaga ttctcaatga
     3541 ttgttccact aaagaaaaat gactcttgag gcaaatatgt cactcgatcg cgtaatatct
     3601 gaaaaggaat gtctaagtga ttaatgtttc cataatggat cgatccttct gatgcttcat
     3661 aaaaacgaac aaataacttt gcaagagttg attttccaga accactaact ccaactatag
     3721 ccgttttact gtgcgaagga attgtacatg aaatttcgtt caacgttggg gccttcatgc
     3781 tatacgagaa tgaaacgttt tttacagtga tatcttgttg aaataattgt tcagaaagca
     3841 tatattcaaa agactggttc gatttttctg aatcaatcgc gaaaatttca tttaaacgat
     3901 tatttgcaac ttgagccgtt tgcattttca cttgtaaatt aataatgttt tgtaaaggat
     3961 ctgtcaaaaa tactagtaaa gcattgtatg tgataagttg acctagacta attctattat
     4021 ccataacaaa ataagaacct agccataaaa ttaatgcact gctaattaat tgaatcaaat
     4081 gtttaattcc ttgttgaata ttatcaagcg taaccgtacg aaatgatttt ttcattaatt
     4141 taacaaattc atggtctata cggttatata ctttctcttc accgctatat gctttaattg
     4201 tttctattcc tttaagactt tctatgatac tagaatttac agctgcaccg gctgccattt
     4261 cttccctgtt cgatttttca tagcttttca cgaaagcgaa tattgcaact acataaaaag
     4321 gcaaagagca tagagtgatt aaaaacaacg tactattctg aatagctaac gtaaccccaa
     4381 caagcaacac cataccaata tctaaaaata ttgataacgt agcacttgct aaagcatcaa
     4441 taatcttact ggcatcaaga aaacgagaaa taatttcccc tgattttctc gtagcaaaaa
     4501 aattcattgg taaagttaac acatgtttga aatactgaag cataattgcc atactcattc
     4561 gctgtcctaa aataataaga agataactac gactatattc aaacaatacc cgaaataaat
     4621 agacagcgat caagccaatg gaaattatat ttaaagttga acgtgcttga ttagggatga
     4681 aaaaatcaag aataccttga aaataatatg agcttcctat gccaaaaaac gtaatgaaaa
     4741 tcgatgctaa aacgatgtgg aatactaatc ctttttgttt ccaaattata ggcaaaaaag
     4801 aacttaatcc agctactttt tctttaatag gaatataagt ttctctaggc ataggtaata
     4861 aaagaatacc tgtccattct ttttgaaacg cttcaatcgt ttttctattt tttccattag
     4921 cgggatctgc aatccatagt acgtttccct tcgttttata aattacaacg tagtgcatat
     4981 agctgttatc aaccaatata tgtgcaatta atggtaagat aagatctctt tctttccaga
     5041 tctcactatc tgcttgaata gccatacaat caaaagacaa tttttcaaat gtctttttca
     5101 atccaaatgc tgaagtaccc tctaaatcag tcccagatag gtcccgtaat ttatgaatcg
     5161 ggatatcagt tttaaaatat tttaaaatca tcgaaaggca agcaacacca caatcttttt
     5221 catcttgttg ctgcacaaaa ggaaatgatt ttatcactta tctaacacaa ctccttctaa
     5281 aaaattttta tagaaaaatt ctcaatatag ctttaataca gtgaaaatat tgtctcataa
     5341 ttccacaaaa actttgaaaa cagttcttga acactaactt tttcattcct gaatcaaatt
     5401 tattttggaa attttactat tcaagaataa acttgataag tttcaggaag atttttactg
     5461 ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata ttatttaaag aagcgtgttt
     5521 aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg cacaaactgt tggtggaggc
     5581 aataatgctt ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg ggcagtaggt
     5641 ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg gtgttgttgg agggttatat
     5701 ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata aaaaaggaga
     5761 ataagtagta tgcatattaa aaacacaaaa acaacattta tattatcttc agaagagttg
     5821 aaaaatattc aaggtgggag tgccgtggga gttttaggca ctactttttc tggggcaaca
     5881 gcaggagtaa aactttgttc agccggagga ccttatgcaa tagctgcttg tggtgtaggt
     5941 ggagctttat taggtgctgg attcagcatg tggaccggtg cgtaaacaat taaaaaatat
     6001 caaacctcaa gattggtttg tgtcgattac gaacataggt atgttcttta tcttttcaaa
     6061 agtttggaat ggacagaact ttttactaaa gcttcttgtg ctagttttta taattatatt
     6121 aaatttttat cttttattaa agtcagataa aaattctttg gaaatgtaat ggtagagata
     6181 tttttacaaa aggacttgca gcgctgtagt tggtgctgca ggaggaccta tttcggccgt
     6241 tacaggaggt attggtaatg tacttgtcgg atgtgcatta aagcctgttc ctgcaagtta
     6301 aatttataaa aacaggaaag taattatctc aaatcctatc aagtacaatc ataggattgc
     6361 acttgacagg acaaattttt taactaaaaa atgaaaaaca ctttctaaaa attagtagaa
     6421 tttgccaaat ctttcgcatc ttccaccata catttaattt taattcccct ttatggtact
     6481 taataaaaac aagaaaagaa ctattcattt ggcccttttt tggttccaga ccatagaaat
     6541 attcctccag taactaatat tgctgttatg cacgtaacta ataacagcta taaaaattgg
     6601 tacaacttta ataggattca gaaaaatttg cactagtgaa ggaatctatt ttattagaaa
     6661 aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact tctccttaat
     6721 tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat atagtgaacg
     6781 ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc gactaattct
     6841 tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt cgtactgccc
     6901 atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa aataaatgtc
     6961 atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt gattcgtaac
     7021 ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa tctcgtttcg
     7081 atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc ttctttttat
     7141 tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt gaacatcata
     7201 ttttctgatt acttctgcta attctatacc gttaacttca tgatttaaat caatatctaa
     7261 gaaatatatt ccttgctttg gtcggaattg tttgaggtat tttttaactt ctaacggact
     7321 ttgtgttttc aatactatct taaatacttt atcgtgaaaa agaatgaaat tatcaataat
     7381 tcgttctaac tgatttaatt gtataatttg atcttcacat aatattattg gataactcat
     7441 aaatcctcct tccctcaaaa caacaaaata acttgagtag taaattgata cggatcgttt
     7501 tgatagttca caaacatatt gggattcttt ttattgattt cctctatact agataaacct
     7561 aacccttgat gtcccttttt tgttgttatt ccttttttca ttagagtatc tatagagagt
     7621 agtatctctc tacaactatt cgatatcgaa atttctatct gtttatcatc tcggtaaata
     7681 gctagagaca gttctctatc ttgactctct atagatgctt caattgcatt atcgagaata
     7741 attccaagca ccctaataca gtcaaatatt ggaacaggaa cttcaataat agtacttgga
     7801 cattcgaatt tacaacaaat cttattgtta tttgcttggt atagcttcgc tatcaacaaa
     7861 cttttcaaat attcattttt tatatttttt aaataacgat attcaaactt attggttaga
     7921 taagaattag aatagatctc taattctttt atttcttttt ccaatatcga atcatggttt
     7981 tgattagcga tttcttttaa actcagcaat aaatttttat agtcatgtct aaatctggca
     8041 aatttttctt gatctttttc cagtgaatca gtatattttt ttagataaac caattctttt
     8101 cttttcaatt gctgttcata ttgttctcgt tgcctaatag ttattctaat aaataaaaat
     8161 agaacaacaa acatttgaat gattaaaaat attaagacac caaacacaaa atgatcaaat
     8221 acttgatatt gatgagcaac ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg
     8281 aatatagcag taagtttaga actggactgt tcaacaatcc tatttatagc taattttcta
     8341 tataaccaaa taaacatcag caaaaacaaa gaatcaagca ttatctggaa tactacataa
     8401 ccataccctt ttatcccttc aaatgaaaac atatacgtca taaccatgga agtaataata
     8461 gatctgaaat catcaataag catacataat agtaatttat ttaaaagcac tatattagtt
     8521 ttcttctttt cctttaatat aaaataactt ccgattatag gtatagctgc ggaaacatca
     8581 gtaaataatt caacaaaaaa agtgagtggt atcaataaac atacaaaaag atattttctt
     8641 ttgttaagat cagtattagt catataaaat aagagaaaaa agaaaaagtt tcccacgaac
     8701 attcttaata gataagcttt tgtatcgata gtaatcaaga gatcccctcc tgattttagg
     8761 atatcataaa aaaaatttta gttacacttt ttaaataatg aaaaaacaca ttccactaaa
     8821 tttgatgccg gttttccttg aggttttgtt ccagcacctc ctttaatttt tttaagctct
     8881 tgatctgaac attgtttagc atttagtcta tttttttctt ccattacgat tccccctaca
     8941 aactataaat acttttatgt cactgattca atatacctat ttttctaa
//
LOCUS       DAJSBC010000092         8954 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSBC010000092 DAJSBC010000000
VERSION     DAJSBC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.094"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(162..341)
                     /locus_tag="ONT65_002425"
     CDS             complement(162..341)
                     /locus_tag="ONT65_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330019.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(722..1429)
                     /locus_tag="ONT65_002426"
     CDS             complement(722..1429)
                     /locus_tag="ONT65_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330020.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1422..1859)
                     /locus_tag="ONT65_002427"
     CDS             complement(1422..1859)
                     /locus_tag="ONT65_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330021.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1874..2125)
                     /locus_tag="ONT65_002428"
     CDS             complement(1874..2125)
                     /locus_tag="ONT65_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330022.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2125..2331)
                     /locus_tag="ONT65_002429"
     CDS             complement(2125..2331)
                     /locus_tag="ONT65_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR2330023.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2768..3028)
                     /locus_tag="ONT65_002430"
     CDS             complement(2768..3028)
                     /locus_tag="ONT65_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330024.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3103..3306
                     /locus_tag="ONT65_002431"
     CDS             3103..3306
                     /locus_tag="ONT65_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR2330025.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ONT65_002432"
     CDS             complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ONT65_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2330026.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3935..4834)
                     /locus_tag="ONT65_002433"
     CDS             complement(3935..4834)
                     /locus_tag="ONT65_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2330027.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4847..5440)
                     /locus_tag="ONT65_002434"
     CDS             complement(4847..5440)
                     /locus_tag="ONT65_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2330028.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5516..5665)
                     /locus_tag="ONT65_002435"
     CDS             complement(5516..5665)
                     /locus_tag="ONT65_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330029.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5681..7885)
                     /locus_tag="ONT65_002436"
     CDS             complement(5681..7885)
                     /locus_tag="ONT65_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2330030.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7896..8918)
                     /locus_tag="ONT65_002437"
     CDS             complement(7896..8918)
                     /locus_tag="ONT65_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2330031.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT         2298 a         1851 c         1539 g         3266 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaagtgcta tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt
      181 acctcaaatc ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg
      241 gattcacttt tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc
      301 aacgtcacgg gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat
      361 cgaaataacc ctattaaaaa tatggctgca atcaacttga atattagact cacaagggtt
      421 aacacaaagc gccaaaagat caaacaaaat accagtattc ctaagaatac tacgaagttt
      481 aaatgtagag ctgcaccatg acgcataagt atctcgccaa caaacagtac gaacaataga
      541 ggaatgattt tattcatatg gatctccttc tttctcttag gttatcgggc tttgcccgct
      601 tatttcgcac cacctaagcg gcgggggcgg ttggatcgat tggtgcgaaa taagaaccat
      661 tcccttaatt tcaaggtttt ttgttcttat ttcacacatc ctcacttttc ggtataaatg
      721 gttattgacc ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat
      781 tttggatttc ttctgtatcg tcgtaagtgt aggtgtctaa aaaatcagtt aaagtcgtgt
      841 accagtcgtc gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta
      901 cagtgagttc ataatccatt cccattaaca cgatagattc cgtcccacac gtcaaaaatc
      961 tagtaaatac aaggtaataa tcgtctaccg tcgcatcttt gctgtttagc tcttccattt
     1021 cttcttccat taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt
     1081 gtttcctgca aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa
     1141 cattcatgat atatttttct gtttctagtc gtagaagctc cgctcctttt tttaatgtgt
     1201 caggacgacg tttatacacg tctaaacgat tatggctgac tgtaaccttc gtttgactcg
     1261 tttcactttc catatacaac tgttcttcca ctctttgaat aaagtacttc atttcttcca
     1321 tacaactaaa gtctttttga atccgtctga ttacttcatt caagtgttct ttgtttgtta
     1381 aaaattgcga aacatcttct gttgttagct gataaaccac cttattcatt atctttctcc
     1441 tccgattcaa tcgtataaaa tgcaccattt ttttcgatat tttcaaaagt atctagccag
     1501 tcgctagcca ttgctttagc tttcttaatc tgtgcgaatt ttgcataagt cagttctaag
     1561 gcttgatatt cggaaggttt gatagtgctt ggtttgacac ctacttccaa gcatagtaag
     1621 gcttggaaaa accattttcc attttgtttt ttcagttctt ccagtgttcg atagtacgtc
     1681 gcaatgagat tcatttcgac acctgattca atcatttctt ccactgcgac tgacaatttt
     1741 tccttgtaat gttttttggg tacacgtgtt tcatggtata ggaacgtcat atcctccatt
     1801 ttttgtgcca ttttatccaa aggtaatttg acaaattcgt catagtaatt ttgagccata
     1861 ttttttctcc tttttagttc tctaatttgg ttaaccaata ttcgatttca taagtttcca
     1921 tgttttcttg ataatgcagt tcatatcctg caatcttcac taggtcttca aatagcatag
     1981 tcggtgcatc ctctactaaa ggggtttcta tcgtccagta ccgcggaata agtaaagctt
     2041 ctgctacttt ttcagaaatg acttctttgt ccaccaaaat ttgaacgact tcttttacag
     2101 tcaataattt gtgttgtgtt ttcatcaccg tttctttact ttctcatcaa tcaaaacata
     2161 atcgccataa gacaaggtat attttttccc gcatgttgga cattgataca aacgatagct
     2221 tttgatacat ttcgctcttt ctccacaatc gcattcgaca gtatgtggtg catgatagtt
     2281 tgggaataat tcgctgaagg gaacacccca ataatcaaat ttctttcgca tgattgattc
     2341 tgcctcctct taggaaaggt tttatttcct ttcgttagtt ctttctttat tgatttgttg
     2401 gccagaatga atcaacgaaa agcgacaagg acaagggcgc gcagcgtgcc atttaccctt
     2461 gtccttgtcg ctttttggtg ataaaaggca acttgttgcc ggtctggcaa atcaataaag
     2521 agatagcgaa caggaacgaa ccttttggtg agaggaagtt ttttctattt tgtcttgctt
     2581 tgcaagacac catatcttag tgaacgtgca acgttcactt cctttcttct cgaacggcgg
     2641 tggcggttgg agtgagagaa aggtttttca attgagaagg cacttgtgtc ttcctaaaaa
     2701 ggtcttttcc tgtttctatg taaaacaaaa agagctgcaa tcctcattta ggactgcagc
     2761 tcttctttta tatgcccatc gatcgcgtaa aatgatcggg gtttctaatt tgcatcgatt
     2821 caatgactaa ttgattccgt ttattgaaat gacctgtgac ggcaatattg tattttccat
     2881 catccataat taacaattta tcgataattt caatatctgc aactacacaa ttaatcgatt
     2941 catgaattgt ttgtaaagta aagcgaacca ataatggttg tgtttttatt actcggattt
     3001 tatcaagcaa accaacatat ttgttcatca cgatcacctc gctaaaatta tagcaaaaac
     3061 agctaaaatt gagaattttt attcgatttt agatatgcta taatgatcaa gacagtaaag
     3121 tttgtgacgg tggctatccg attgaaagtg aggtggtgcg tatgtgcgta cttccaaaat
     3181 cttacagaaa ggagaagcct tttgcctgtt gaaacggcgc taggattaat gattagtttc
     3241 ggttctttga tcgcaacaat catttttgga atcttagcag ttgtaaacaa caatcaaaaa
     3301 aaataaccgt cagctttagc cggtcgacga ttatttttca aaaataatat taagctggtc
     3361 accgtcttaa acggtgctgt taggggagtt gttagcgcaa ctcctctttt cgtatccatt
     3421 ataacaactt tattgatctt ttacaatcag aatggtaaat catcatcact aatatcgatt
     3481 gattgtccgc caaatggatt ttgtgaagcg ttcaatccat tgttgttagg cgtagctaca
     3541 tcccgttgtt caaaggtggt agggcttgga ctgctggacg attgatcagc gtgcgcacgg
     3601 ctttctgtcg ctgctttact ttctaatagt tggaaattat cggccacaac ttctgtcaca
     3661 tacacacgtt gtccttgttg gttatcgtag gttcgggttt gaatccgtcc aacaacgccg
     3721 atcaaactgc cctttttagc taatgtcgcc atcgtttctg ctggtttgcg ccaaatcaca
     3781 cagttaataa aatcagcttc tcgattgccg ttttgattcg tgaaatttcg gttgacagct
     3841 agggtaaaag tcgctactgc agtaccactc gcggtataac gtaaatctgg atctttggtt
     3901 aagcgtccta ctaaagttac attattgatc atctttatct ctccatttct tgattcattt
     3961 gttgtgatct tttaaactgc cgttttcttt cttgattttc aattttgatc acacttttca
     4021 ttaggtgatc aatttcgttg actgtatctg taaatgaaat ggaattcgca aacgtgttaa
     4081 ctgagcgata tttttcccac tgcttttcca aatagtcatt tcctctgatc ttatccattt
     4141 ctctttcata ataaaaggac aacggttttg tatccccacg ttttgtaaat gtatttcgaa
     4201 cacttttgta taactctttc gtatcgatat tttctagttt cggaatggta aataagtcgt
     4261 agtaatcttt tgctctcgaa ttatctgtgc caaagctgaa cgtagcactt agcttttctg
     4321 caataatttg ttctgttgga taagctggaa tctccacttt tttatcttca aacattaatt
     4381 ggtgatagtg tgtatctact tcaggataaa tagaatctcc tgttgtcacg tccattttaa
     4441 acggtacacg tactttctct aaattagcca ccaatttaat ttgaaatcca ggataaaaat
     4501 cggcttctct tgtttccttc attccttgaa tagagaaaac aatgccttct tttgtgggtt
     4561 ttctagttaa atcattaaaa atgctcttca atttttcttc agttaatttg ctatttctaa
     4621 atgtcgtatc gatatcaatt gttgtccttc tatcgattcc atattttgat cctaataaaa
     4681 agccgccttt caaaatgaag tcatttttgt actttgattc agaaatttgt tcaattagac
     4741 gatccatcat aaaatgccgt tgcactaact gtgcatttaa tcctgtactc tttgctaaat
     4801 ttcttgtttt tgctgcaaat tgtgtcggtg tcatcttttt cctccatcaa tataagcttt
     4861 gcatgtggta tttcatttct ttttctacat gtagttttgt catgtaatct cttaatttac
     4921 ttggatctct caatgggtct tccatgtact cttttaatgc ttctaatttc atttcatatt
     4981 caaattcata tctcggattc caaatatcac aaagtgtacg ctctttatca tacatagtaa
     5041 taggatttcc ttgaaaggaa gttccctcta cttttcctag ttcataaact tctttcttca
     5101 cgaaatgaaa ttgtaactgc tgctcttttg taattgaacg atcaacgtta tagccatggg
     5161 gaaaagttaa gtgaatgaat ttcggcatat tcgtagaaag ctggtaaata tctaaagtcg
     5221 tttcatgaga gaagattccc ctggaaaatc gttgctgaag ccaaaataaa tcatctattc
     5281 catgaccatc ctctacataa taaccacgat caactttgat caattttcct tgattcacca
     5341 atctttgtac agtggttgct gcgattccaa atttcctggc ttccttcata gataaatttc
     5401 catcgcgaat agccaacaat tctttcagtt tatcttgcat ttacttcccc tcccttcgtt
     5461 acaaatacac tacatcaaat atatcatagt agtttatttg taacaagatt aacgtctatc
     5521 gatctaattc gatttctttt tgaatgcgct gctggttctt taattgttgc agtcttcttt
     5581 tatttgcacg taatttctct tgttctaaac gaatttttgc tttttgattt tcgatttctt
     5641 gttgtttgtg tgttgtattt cgcataggat aaccccttca ttattttgta gtgcggcgtg
     5701 ttcttttctt atagttcgtt tttttctttt cagaaaattc aaagcctgtt gtaaagtctt
     5761 cttttaaaac gatcgttgct tcaaacgaac ctttctgacc ttttagtcct ctgactgatt
     5821 ggcttgttcg gccgttttga atgatctctt tcagttgtgt ggctgtgagt ttctttttgg
     5881 caatcgttgt ccataattta aaatcacagg cttcagacgt acatgccgcc acattttgat
     5941 aaagcaagac ttcttgttcc cgacattttg gacaaacacc taaatgacgc gttgcttcct
     6001 gtttttcact ggctgctttc acctcttgta acgttgatcc aaaatctgtt gactggattt
     6061 gtcccggtac gacttccaac aagtgagaga caaatttttg gatattagct aagaaagtag
     6121 cttgtgttcc tttctgctca ccaatcttta ataagtaact ttcccatttg gcggtcattt
     6181 cggcacttgt tagcagcggt tctttttcaa cagcctgaca aagcaaaatt cctttattcg
     6241 tcaccacaag tttatttttc tctaccttga tatattcctt ttgcttcaag gtttcaataa
     6301 tatttgctcg tgtcgcttct gtaccaattc cctccacttc tttcaatatg gattgtgctt
     6361 cttcactatc cacggtttta ccagctgtct tcatggcggt gattagtgta ccttccgtgt
     6421 aaggttgtgg tggcttggtt tctttttcaa agatttgtag atctaccgtc accgtttctc
     6481 cttttgttac tttaggaagt agcggttctt cttccttctc ttttctgtcg ctctctttgc
     6541 caaatagctt tttccaacct agatctactg gtacttttcc gactgccttt agctgaagct
     6601 gctggacttg tgtcaaaatc gtagtttctt cataggtata tttttccgca aacatggcca
     6661 ccgtcgtctt taaaacttga tcataaatcg cttgttgtaa aggtgtcagc ttttcatacg
     6721 ttgcttctgt cataactgtt tttgtaggaa tgatcgcgta atgttcctgg actttcttat
     6781 tattcacata ccgactattc ggtgtatgaa taggagtagg gaatgattca gcctttaaaa
     6841 aatgcttgta ttcatctaaa tgatccaaca gatacgcatg ctctccttct gtaatataag
     6901 gggtgtctgt tcgtggatag cttagatatt tcccttcata caacccttgc atggcttcca
     6961 aagtatcttt ggcactagct ttcatgagct gattcatttt agactgaagg ctggatagag
     7021 aaaaaagacg tggacttgcc gcgctttttt tctcggtttc gacttcaaga atcgtgcctg
     7081 gctgctttcc tacttgaaca cctttttctt ttaaataggc ttcaaatgct tcttgcgtcg
     7141 cttgggtttc attgggatca agcttcgcat caaatatgcc attttgagcg gttacttggg
     7201 ctttcccttc aaaataaggc tctttttgga agtttctaat cttttcctgc agctgataaa
     7261 tcatgtaaag cgttggggtt tggactctgc ctaaggaaaa acttcctggt atgccctttt
     7321 gctgcaagag taaactatac agtgggctgc cgttcatgcc gatcaaccaa tcagcgatct
     7381 ggcgtgcttg tgcttcttca aatttgggat aggtctcttc tgcgtctcgt aattgctgga
     7441 atccctgata aatggcttct ttttctaagg agttgatcca aagtctttta aaggtttttc
     7501 cttgatcagc gcctgcttgt tgaatgatcg tccaagcgat cagttcccct tcacgatcgc
     7561 tatctgtcgc aataataatg gtgtcggccg attgaagttg ctgtttcacc acgttgaatt
     7621 gctttttctt atctttagga acatgatagt gataatgtgt agggaaaata ggcaaatgct
     7681 ctaatgacca ttgtttccaa ttttcatcgt acatatcggg cgaatctaac tcgactaaat
     7741 gaccaaaacc atacgtgatc acagtttcac ccgaaaacaa tcgatcttgg atttcaaaat
     7801 acccgtcttt tcgggtggct ttagaaaagc tatcggcata ggccttcgct tgtgacggtt
     7861 tttctgctaa aatgacagtt ttcatcgtga cgatctcact ccctttcttg ttgtctgcct
     7921 tgttgaaaca acatcgcacg tttttgcttt ctaatttcct gttgatggat ctcatgcttc
     7981 aattgatcgt tccaatcctt tttcacttca ggtacaaatt catgttcatg atttttatag
     8041 cctttgtagt attgttctgc tttataagag aaaagaatct gagaattaat atcccctttt
     8101 ccttgataaa aacgatcaaa atcatacgta ccattcgagc gttcacaatc ttttaattct
     8161 tttgcgactt ctctacaaat atctttcaaa ctaatgactt ctattgaatc gttaacagaa
     8221 agctgctgac caaaagacgc attgactttg tgagcggaaa ccagttggtt cgttaattgc
     8281 agattgtttt ctgtcttgtg tatggccata atagcttctg gtgtgacctt ttcttctttt
     8341 gcgacttctt cataaatgac tttgtattct cttggcactt gccaatcatt agcgattcct
     8401 cgttcacaac gtactttctg attcgtaaat ggatcaactg cgcccattaa ttgttctttt
     8461 tcgtagaaaa tatgtcctgc acggtcatta tccacacaaa tctcgatgga ttgaggaaag
     8521 gcttgtttct ttctcaattc agatactgcc tcaccaaaat aatgactgat cacatggtgt
     8581 tttaaccctt ccatagagac taaccacgta tcgtttaact gatcacgatt cagtgcagcg
     8641 tagctaagta aatcaatact actctcaaaa aattttaact gtttaggatc accaatcttt
     8701 aggttaaagc catgattcgc tgtcgaattt ttatctatat gcttatatgt gcctctctca
     8761 ccaaaacgtt cataatcaat gaacgttcct tgataactcg cccctactgt ctcttttgtc
     8821 tgtggatctt tccataaaaa ggcgaccgca tgatccaaac gatcgtctcg aactaagaaa
     8881 ttttgccgtt catgttttgg ctttagttcg gcaatcaatc cttctttttc tgcataatca
     8941 atcaaccaat cggg
//
LOCUS       DAJSBC010000093         8920 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSBC010000093 DAJSBC010000000
VERSION     DAJSBC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.095"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..777
                     /gene="menA"
                     /locus_tag="ONT65_002438"
     CDS             <1..777
                     /gene="menA"
                     /locus_tag="ONT65_002438"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2330032.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT65_002439"
     CDS             complement(1712..3058)
                     /gene="gor"
                     /locus_tag="ONT65_002439"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR2330033.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3192..4445)
                     /locus_tag="ONT65_002440"
     CDS             complement(3192..4445)
                     /locus_tag="ONT65_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2330034.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4451..5374)
                     /locus_tag="ONT65_002441"
     CDS             complement(4451..5374)
                     /locus_tag="ONT65_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2330035.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5595..8087)
                     /locus_tag="ONT65_002442"
     CDS             complement(5595..8087)
                     /locus_tag="ONT65_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2330036.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8094..8561)
                     /locus_tag="ONT65_002443"
     CDS             complement(8094..8561)
                     /locus_tag="ONT65_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR2330037.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8697..8770)
                     /locus_tag="ONT65_002444"
     tRNA            complement(8697..8770)
                     /locus_tag="ONT65_002444"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8734..8736),aa:Arg,seq:acg)
     gene            complement(8828..8901)
                     /locus_tag="ONT65_002445"
     tRNA            complement(8828..8901)
                     /locus_tag="ONT65_002445"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8865..8867),aa:Pro,seq:tgg)
BASE COUNT         2479 a         1884 c         1485 g         3072 t
ORIGIN      
        1 aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt
       61 ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt
      121 gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga
      181 tttatctgtt gttttattgg agtattctat acattcggac cgattccttt atctcgaatg
      241 cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt
      301 tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa
      361 tttgatttgg taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg
      421 gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt
      481 gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc
      541 cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat
      601 gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa
      661 ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta
      721 ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag
      781 catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat
      841 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      901 gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
      961 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1021 ccgtttatca gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
     1081 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1141 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1201 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1261 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1321 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1381 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1441 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1501 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1561 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1621 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1681 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1741 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1801 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1861 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1921 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1981 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     2041 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     2101 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2161 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2221 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2281 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2341 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2401 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2461 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2521 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2581 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2641 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2701 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2761 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2821 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2881 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2941 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     3001 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     3061 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3121 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3181 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3241 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3301 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3361 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3421 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3481 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3541 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3601 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3661 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3721 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3781 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3841 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3901 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3961 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     4021 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     4081 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4141 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4201 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4261 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4321 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4381 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4441 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4501 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4561 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4621 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4681 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4741 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4801 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4861 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4921 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4981 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     5041 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     5101 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5161 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5221 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5281 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5341 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5401 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5461 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5521 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5581 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5641 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5701 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5761 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5821 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5881 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5941 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     6001 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     6061 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6121 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6181 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6241 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6301 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6361 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6421 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6481 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6541 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6601 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6661 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6721 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6781 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6841 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6901 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6961 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     7021 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     7081 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7141 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7201 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7261 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7321 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7381 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7441 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7501 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7561 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7621 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7681 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7741 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7801 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7861 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7921 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7981 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     8041 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     8101 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8161 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8221 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8281 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8341 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8401 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8461 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8521 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8581 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8641 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8701 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8761 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8821 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8881 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DAJSBC010000094         8873 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSBC010000094 DAJSBC010000000
VERSION     DAJSBC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.096"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..718)
                     /locus_tag="ONT65_002446"
     CDS             complement(<1..718)
                     /locus_tag="ONT65_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2330038.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIR"
     gene            complement(724..1830)
                     /locus_tag="ONT65_002447"
     CDS             complement(724..1830)
                     /locus_tag="ONT65_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2330039.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1830..2966)
                     /locus_tag="ONT65_002448"
     CDS             complement(1830..2966)
                     /locus_tag="ONT65_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2330040.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2998..4449)
                     /locus_tag="ONT65_002449"
     CDS             complement(2998..4449)
                     /locus_tag="ONT65_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2330041.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4493..5257)
                     /locus_tag="ONT65_002450"
     CDS             complement(4493..5257)
                     /locus_tag="ONT65_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR2330042.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5285..5983)
                     /locus_tag="ONT65_002451"
     CDS             complement(5285..5983)
                     /locus_tag="ONT65_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR2330043.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5995..6774)
                     /locus_tag="ONT65_002452"
     CDS             complement(5995..6774)
                     /locus_tag="ONT65_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR2330044.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6790..7734)
                     /locus_tag="ONT65_002453"
     CDS             complement(6790..7734)
                     /locus_tag="ONT65_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2330045.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7780..8559)
                     /locus_tag="ONT65_002454"
     CDS             complement(7780..8559)
                     /locus_tag="ONT65_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330046.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2534 a         1840 c         1317 g         3182 t
ORIGIN      
        1 ctctaattat agcaaacatc catctagact cttgattaca tgctaatgaa taacccgcaa
       61 aatatcctga tgctacggat gcgtgagcat aatcaggcct cctaatagac caaatatcga
      121 tatcaaaata ctcatctggt attggttttg tacaataaaa tgtggcgtct aaccacaaac
      181 ctccatgttt tgctagcaaa gataatctta ataaatcaga ataatttgtt ctcgtaatga
      241 ttcctttgtt tttcttttct tccacccatt ttggtatatc aacatattgt ctataattat
      301 cttctgttaa aattataaca gtatgtttcc ctgcatttcg ttttattgat tttacacaag
      361 cttttacaat ttccggcgat tgatccaatc cctgccacca acaaacccaa atgcagttag
      421 aatatggtga ttttgattga ttaattattt gattttgact ataatcatat gttttcaaaa
      481 aatctgaaaa agtcttttca taatacttca gcattacctc atgttttttt aataaatgtt
      541 ccttctgttt tttactttca ttatatccat tacgattcat gacttgaata tcaattttag
      601 cttgtaacgt ggcaaatgct tccttccaag atgttatctt tgcaattcgt cttgttgcat
      661 tcaattcttc ggatatgcga tgtataattc tttttatact tgaatgtttc tgtgtcatat
      721 tctttaacct ctttgtatta attcaaataa ctcataatat tttgattcta agattttgta
      781 tgctgtttga atattataat ctgcctttat taaaatcgaa cgttctgaat ctcgatgaga
      841 gatatctaca tttgcaatct ctctcgccca ttcttttgcc gattgattta aacttatgaa
      901 attggtctta tcactgaaag caacttctgt aggaactttc tccgaaaaga aacacggtaa
      961 atctgcaaat tgagcttcaa tacccactaa cggaacccct tcaaataatg atggcaacat
     1021 taatacgtca aatgcttgat aaaatttttc tatatcattt ctatttccta aaaatttaat
     1081 atcatcatct atgccacttt tctttgcata attttttatt tcttcttcca tatcaccagt
     1141 tccaataatt aataatacgg aattattctt aatagcgtga aattctttaa aaatttcaat
     1201 caaaaaacga tgatttttag aatatgaaat tcttccaata tgtcccatta caaacgtctg
     1261 acttgaaatt cctaatttca ttcgtacatc atttctaata ctttcatcat aataaaattt
     1321 tttagcatcc actgcatttg gaatcaaagt gaatttatca tttcgaaaca aaaaatcacc
     1381 tgctactttt ccacatgaaa attcatgtgt caaaacagac gttaacttac atcgataata
     1441 ttgctttaaa agatatttaa aatctctatc tatcgatgta ttatggctat gtgctatccg
     1501 tatcggaatc ccagcttttt ttgcagcttt taaaggtaaa taactcatcg catcaatatg
     1561 agaatgaaca atctcatatt ctggatgatc cttaaaaaat tgttccatcc atttaaaata
     1621 tttaggataa ttttgtggat aaagtctcgg tgcataatag acctttcctc caagacttat
     1681 tatttcctca tcataatcac ttttatatgg acgatgtgtc aaaaaatcaa actgaatttt
     1741 agttcgatct atattcctat aatagttcat tagcatcgtc tccaaccctg cacgatgcat
     1801 atcattcaca cactgtaata ttcgaatcat tatatatttc ccctatacaa atttaatgtt
     1861 tcttgtacga ttttatccca attatattta ctacaaataa actcagtagc ttgattcttc
     1921 aaaactttaa ccatttccga ttgattgcat gcctcttcta atcttatctt taaatcactt
     1981 acatccgatt tcttaaaaat catagccttg tcttctacta cttccgtaca ctctgagata
     2041 tttgaaacta tacaacagtt cccataactc attgcttcta acaaacttaa tggcattcct
     2101 tctaaatcac ttggaagtgt atagatatac gcattactat ataattcttc taattcctgt
     2161 ccttgtacaa atccagtaaa tataattcgc tcatcacctt ttgctaattc ctttagttca
     2221 tttgcaaatt catctgtatc agagcttcct ccggctatta taagtttttt atctgtctga
     2281 acatctttaa atgcctcaat tagataccga attccttttt cgggaaccaa tcgccccaaa
     2341 aacaaaatat aagaatcttt tgttaatcca tacttttctg taatcaatcc tgctgatttt
     2401 atttcaattt ttttaacgcc atttggtata aaccttgttt ttcttccata tatatcttca
     2461 aaataatttt ggacgctttc actaagtaca attatttcat ctgcaaattt tacagcacac
     2521 ttttccccta acatgatgta tgtactagca agcttgttcc atttttctct ttgatgatct
     2581 agaccatgaa ttgttgcaac acatcttttc ccaaaaagtt ttggcaacca caacatagca
     2641 catggtcctt ctgcatgaaa atgaacaaca tcatattttc cgaatgctgc tctaattcca
     2701 ccaaatactg aagaactcat tgctgcaatt ccttttatat ttaatgtaaa aatagtcttt
     2761 aattttacac ctttatattc tttattttta tgctggtcaa attcctttcc gctaacatga
     2821 tgaccacttc gattatagca agtcacttca tgcccaagtg caaccattcg cgttgccaat
     2881 tcttctacca ctatctcaat tccaccttct cttgatggaa ttcttttatg acctagcata
     2941 cagattttta atttttttct tgccatcacg ttttactcct tctatagtat ctttatctta
     3001 catggaaccg tttctatgaa gcacagtttt gactgtccta aacaaaattc taaaatctaa
     3061 tcccatactc caattcctga tatattctgc atcaagtttt acaacctctt cgaagtctgt
     3121 gatatcactt ctaccactca cctgccacat tccagtaatc ccaggtttca tcgcaattct
     3181 cgcacgatga tgaaaatcat acttctcata ttctgacttc aagcaaggtc taaaccccac
     3241 caggctcatt tttcccatta ggacattata aaattgtgga aactcatcca atgatgtaga
     3301 tcgaataaat tgtccaattc ctgtcttctt tgttccatca ggaagtattt tattgccaat
     3361 gattcttggg tcaaaatcca tcttaaacat gagattatta ctcatcttat tttctttcat
     3421 caactctgct ttgcgttctt ctgcatccat atacatacta cggaacttat acatcttaaa
     3481 tgtcttacca ttttgaccaa tacgttcttg tgcaaagaaa atcggccctg gtgatttgat
     3541 gtaaataact ggtgctacga agataaaaat aacacctgtt gcaatacatc caataattcc
     3601 agcaagaatg tcaatcgttc tctttataaa tgcttgcctc atcgtcataa cattaatact
     3661 agatgtaata acagtataat caccaactcg gccaataaat tgttttccac gtgtactatc
     3721 agacactttt gtaagattca tatgtacagc aagtcccatt tccgaacatc tggcgattaa
     3781 ttctttggga tatggaacac tttcgtcaac attgatgaat acttcatcta cccaattctg
     3841 taaaagatat tccgctgcat tatcagcatt tgcaactact gggacacctc cgatggtagt
     3901 tccatacata tctctgtcta ctacaatcac accactaagt ttatacatgc cataattctt
     3961 ctctttaaga ttacgaacta cattttttgc aatcttttga gatgttacaa taactaacga
     4021 tacatcttca ccgtttttca tgcggtgatg caacaaccct ttccaaagta tacgaattat
     4081 atatgttaat aatccataca ccacccccat tataaataaa gaaactctgg aataatcatt
     4141 tccatcctgc attgtaataa ggtataaact gcacacaagt aacagcataa aagattgctt
     4201 tatagaagct acagcctctt tataatatcc tctttttaaa acatttttat atgcctcaaa
     4261 taaaaatata atcgtaagat tctcaagttc tataaaaatt gccatgtggc gataaatagg
     4321 aatcacatac ggattccagt caccctgtcg cattacatat gatatataaa aagcaagctg
     4381 gacacaaaat aagtctaata aaataaaatc aatatgttta gaccaaccac ttgagttttt
     4441 tctatacatc tctcactctc tcctcatttc ctttttatct acgaataaag aactaaaata
     4501 aaccaaactt cttctttttt acctcttcat agattggata attgatctca tccccgttga
     4561 tcagatcctt tgcgacttgt ttcatctgta ctactttttc cttaccaaac tccttggcaa
     4621 tctgctcata gcactctttt aagtaaaagg tgcgcttaga gaccccgtgt gcatcagaag
     4681 cagccatctg cacgagatta tgtttgacca ttgttttggc tgttttctgg atatctttgc
     4741 caaatacacc aacataactt ggcgcagtaa gttgggccaa acatcccata tctaggaacg
     4801 gaagcaaatg atttgggtct tctcgaaaat gcgcgttgcg ttctggatgg acgatcactg
     4861 gtatctttcc tagttctaac aactcaaaaa aaagccgttc tgcatataac ggcacttcca
     4921 gtgtcggaaa ttcgatcaga agatatgtat catctaaatc tgtaaataaa atttcatctc
     4981 ggtggatgtc ttcgatgagc gttccggtaa tacgtacttc ttgtccttcc aataacgtca
     5041 aaggcaactg tcttttctct aattcggctt gtaacgaagc gactagagaa atgacttgtg
     5101 atttttcatt gctgtatttt ccattattgt ggtgtggtgt gcataaaata tgcgtaatgc
     5161 cttgctgaat cgctttttca gccattgcaa tacttgcttc taagttttct gccccgtcat
     5221 cgattcctgg taggatatgg caatgtagat cgatcatttc tcttgtctcc tctcttctta
     5281 ttccttaatt tccgtagtaa taaccagcat ctttgctgtg ttctgctccg ttgtatacga
     5341 cacctaagac tcgtgcttgt accatatcta acagttcttt tgctttagtc aatgattctt
     5401 ttcgtgacgt attttcccgt acgaccaata aggtgccatc cgtttttgaa gacatgatct
     5461 gtgcatctgt tacggcaacg actggcggca tgtcgaagat cactacatca aatagttgtc
     5521 ttgcttctgc taaaatctga tccattcttg gcgaacttag tagttctgat ggatttggtg
     5581 gtttaggtcc acttggcaga atcgacaaat tgtcgactgg tgttcgttgg atctcatctg
     5641 ctacacttcc tgatgaacta agaacagtac ttaatccgct tgcgttgttc aacttgaacg
     5701 ttttatagat gacgggctta cgtaagtctg catcgactag cagcacccgt tgaccagatt
     5761 tggcaaaaac aacggcaatg tttgctgcag tggtcgattt cccttctcca ggtcctgatg
     5821 aagtcacaac aatcgttttg atttgctgac cagctgcgga ggcaaactgg atattcgtcc
     5881 gaatcgtacg atattgttca gcgattggtg aagagggatt ggtgagtgtg accaaactga
     5941 ctgcatgagt ttgggctgct ttttgtttct gtgttcgtgc cattttcttt cctcctagac
     6001 tcttgagcgg cttctgcgtg atggcgctgt tttatcgttt gtagcttttg gttgtgtctg
     6061 agctggtcct tttttacgga taccagcatt caattctttc gctgacattt ctggaactgt
     6121 tcctaagatt gggaatccta atgtatctgt cacatatttt tcatctttca ctgtccgatc
     6181 gagtaattcc aataagaatg ccagacctac acctaccatc attcctaaca ttaagccagt
     6241 cattaaattc aatttattat ttggcgaaac aggtgtcgta ttggcaactg cacctgaaat
     6301 gatcgagatt ttatcaacat tcatcacatc ttttgcgttc tcttggaaaa cttgtgaagt
     6361 tgtgttagcg atctgttgag cagccactgc attcgtactt attgcctgaa tcgagaacat
     6421 ttgtgaattt tctgcctgat taactgagat ggcatctttg atttgttcag cagtcatttt
     6481 cacattgtct tctgtttcta aacgatcttt gacttcattg agcactaaat cactgacgat
     6541 catatcttta tacgtattga tcatttgcag gttcgtattg acatcattga catttgcagt
     6601 gtctgattgt ggcagtgtca cgatcaattg tgcttgtgaa ctgtattttg gtgtcaagat
     6661 gaaaaatgtt acgattcctg agatggccaa gccagcaaac attgagatga tgattattac
     6721 caagtgtttg cgtaatgttt caaaaatttc tttcaagctg attgtctctt ccattttttc
     6781 tccactttcc tatttattct ctagattcat ttgatctttt aattcttttt tcacacgatc
     6841 aagttcttga tcactgactt tttgatagga gatattatct tgcatgaagc cttctccttg
     6901 cagctgatct tgcttaatcg taccaaatgc ctcccgataa tctaaagcaa tttttttcat
     6961 ttcatcaaag ttcatgtctg ttttcatatt cgactctaga gcattcaaaa cggattgata
     7021 gttgctgaca ccgtttagac tcaatacttt tttcgctacg ccttctacga tctttctttg
     7081 gcgcccttgt ctaccgtaat caccatttgg gtcttcataa cgcatacgag aataagataa
     7141 tgcctgatcc ccgttcaatg taattttacc gattggaaag tcatagccat cttgtgagaa
     7201 agtgaggtca ttattgactt cgataccacc aactgcatca acgagttcct ttagcccttt
     7261 catattgata gagacatagt gatcgattgg gatgtctaca tatttttgta cggtatccat
     7321 cgccatggca gctccgccga atgcgtaagc gtggttgatc ttgtcttttg tgtcacgacc
     7381 tacgatgtcg acatacgtat cacgaggaat gctgacgatc gttgtctgtt tgtcttcagg
     7441 actgactgtg gcaaccatca tcgtatctga tcttccttga tcgacccgac ctaaatctcc
     7501 agtatcaatc cccatcaata gaacagagaa agattcttgt ttgtccagat caactggttt
     7561 cgttcgttct ccttgggttc gttccactga ttcgtaagtt ttctcaatag aatgtgagag
     7621 atccagatat aattttgctc ctactcctac aattgctaat actaatacga taagaactgc
     7681 taaaataatc agaaatattt ttttccctct tgattttctt cttcgtcttg ccatagtttc
     7741 ctccgtttgc taaccttgct tgaatcaacg ttttaactgt caatagctta tattgctata
     7801 gcccatttgc tcagcaacag tttgtgcttt tgttgcgtat gctgcgctta ttttttcatt
     7861 ccggatctgc tctatttgac tgagcaccgt caaatattga tcgtaagtag cggtggcagt
     7921 cacttgacca tcctttaaca tcgtatcgaa taactgatct agttgcgtga cttgcgcgat
     7981 ctgaatatca gcgaatccta gatattgcat aacaaccgtt ttccaaggac tatctttaaa
     8041 aaagctcata ttttcacgta cacgagcgac atctgcactt gtcacctttt ctttgatgat
     8101 gacatcgtct acagcttgct gccagtttga tacattttta gtaaataaat cgttgacagc
     8161 tgtctggatc ttcagttttg attctgcact atctaactgt gtcacgattg ctttcttctc
     8221 atcttggatt gcaacagctt ctttaggcaa ttctgattct ttgatgtcgt aatcatctgc
     8281 tgaaagtttg acagaggata gcttatttga caaacttgtg attgtttctt ctgttatttc
     8341 agggtttaga aaaatttttt cttttgtact atacagcttg tctacttctt ttgataaaga
     8401 agtcaaggtg tctttttgat tggtgatggc agattctgca taattgatcc gttgctgatg
     8461 ttgccgctga ttattgatat ggtatccaaa caaacagatc gcaatagcac ctgcacctac
     8521 cgccacagca gtcaatgact tgacgaatgg cttttccata gattcactcc taagtattga
     8581 tgatagataa attaagatat aattcactta cataaaaatg acagcttttt atttaaaaaa
     8641 acataattaa actaatatat tcacagaaat atttgtaaac aagttttccc tatataatca
     8701 taccacaaac tgttttcttg actagggcaa tttacgattt aaaagtaaat aaaacattaa
     8761 taagataaaa attaatcgat ttcattttta tttccataat tttcataggt gtttttaaaa
     8821 gttacacaaa aaatagaata acaaaaaaat taacattgaa ttaataatag tta
//
LOCUS       DAJSBC010000095         8851 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSBC010000095 DAJSBC010000000
VERSION     DAJSBC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.097"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            63..1277
                     /locus_tag="ONT65_002455"
                     /pseudo
     CDS             63..1277
                     /locus_tag="ONT65_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1785)
                     /locus_tag="ONT65_002456"
     CDS             complement(1456..1785)
                     /locus_tag="ONT65_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2330047.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="ONT65_002457"
     CDS             complement(1775..2047)
                     /locus_tag="ONT65_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2330048.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="ONT65_002458"
     CDS             complement(2498..3301)
                     /locus_tag="ONT65_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2330049.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="ONT65_002459"
     CDS             complement(3279..4403)
                     /locus_tag="ONT65_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2330050.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="ONT65_002460"
     CDS             complement(4419..5234)
                     /locus_tag="ONT65_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2330051.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6096)
                     /locus_tag="ONT65_002461"
     CDS             complement(5224..6096)
                     /locus_tag="ONT65_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2330052.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="ONT65_002462"
     CDS             complement(6181..7470)
                     /locus_tag="ONT65_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2330053.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="ONT65_002463"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="ONT65_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DAJSBC010000096         8758 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSBC010000096 DAJSBC010000000
VERSION     DAJSBC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.098"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..277
                     /locus_tag="ONT65_002464"
     CDS             <1..277
                     /locus_tag="ONT65_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330054.1"
                     /translation="SELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKG
                     IYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(374..808)
                     /locus_tag="ONT65_002465"
     CDS             complement(374..808)
                     /locus_tag="ONT65_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2330055.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1040..1750)
                     /locus_tag="ONT65_002466"
     CDS             complement(1040..1750)
                     /locus_tag="ONT65_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2330056.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1740..2600)
                     /locus_tag="ONT65_002467"
     CDS             complement(1740..2600)
                     /locus_tag="ONT65_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR2330057.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2605..3243)
                     /locus_tag="ONT65_002468"
     CDS             complement(2605..3243)
                     /locus_tag="ONT65_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR2330058.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3258..3557)
                     /locus_tag="ONT65_002469"
     CDS             complement(3258..3557)
                     /locus_tag="ONT65_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2330059.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3600..5084)
                     /locus_tag="ONT65_002470"
     CDS             complement(3600..5084)
                     /locus_tag="ONT65_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2330060.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5104..5565)
                     /locus_tag="ONT65_002471"
     CDS             complement(5104..5565)
                     /locus_tag="ONT65_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2330061.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5583..6650)
                     /gene="ulaG"
                     /locus_tag="ONT65_002472"
     CDS             complement(5583..6650)
                     /gene="ulaG"
                     /locus_tag="ONT65_002472"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR2330062.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6868..7638
                     /locus_tag="ONT65_002473"
     CDS             6868..7638
                     /locus_tag="ONT65_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2330063.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7911..8738)
                     /locus_tag="ONT65_002474"
     CDS             complement(7911..8738)
                     /locus_tag="ONT65_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2330064.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2646 a         1851 c         1378 g         2883 t
ORIGIN      
        1 cagtgaacta cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc
       61 ccacaaagtg acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg
      121 ttcggcaaaa ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc
      181 atttgcttta aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat
      241 tgtatttgat aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga
      301 gtgatactaa gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt
      361 acgttattat atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga
      421 cataagaaat accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt
      481 ttgttttctc agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct
      541 tctccaaagc ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa
      601 tgaggatatc ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga
      661 taatcttccc ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta
      721 accttgcagt gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct
      781 catcgatcat aagggaatca atattcattt ttttcttcct ttcttataca attctccctc
      841 taataataca gttctttgac tatttaatct atcacatata actctcctgt aaagattctc
      901 tcgattcctt aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt
      961 tgtgataaat tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc
     1021 tacccttacc ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat
     1081 aatgtttatc tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt
     1141 ctttggccat cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg
     1201 aatcccccca tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat
     1261 ctaattttct ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt
     1321 ctcttacttc ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag
     1381 catgagtcgt gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg
     1441 tggaatgagt atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg
     1501 tggggagatc tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg
     1561 ttaccaccat ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga
     1621 tgactcctag ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt
     1681 atcgcggcaa tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt
     1741 cacaagacat catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg
     1801 atttctcttc gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca
     1861 taatctagac gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa
     1921 ggaacatcac gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg
     1981 atagcagtga tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat
     2041 gcggacaaat tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg
     2101 atttctaaaa attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga
     2161 atgatgccat atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct
     2221 ctggaattca ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg
     2281 acgcttagat tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc
     2341 ttttctggat cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg
     2401 atacgtacac cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa
     2461 tctaatcgtg ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc
     2521 atttctttta caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca
     2581 taaatcccaa tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt
     2641 ttgtgcggcc ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata
     2701 cacatcgatc ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac
     2761 attaaagcct agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc
     2821 accagataac agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg
     2881 ccaatccatg gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat
     2941 ttctttttgt gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc
     3001 tgctcctgct tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc
     3061 agcgactagt tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc
     3121 ctgtaagcaa agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac
     3181 atctcctaat gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct
     3241 catcttagtt ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca
     3301 ctgatttctt tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct
     3361 gcctgagtaa acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct
     3421 gaactttttc cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct
     3481 ttcattgctt tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac
     3541 gatactaaaa ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt
     3601 tatactctct gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca
     3661 ggatttttcc gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt
     3721 ccagcataac ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg
     3781 aacatcccga tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa
     3841 ccgcctactt gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct
     3901 cggttattgg cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct
     3961 atgacgatcg ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa
     4021 gcaccaaaca agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct
     4081 acgtcaattc ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga
     4141 gttagttcat cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa
     4201 tttaaagaag tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct
     4261 tgaatcaaat aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt
     4321 aacgaagtag caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct
     4381 tcaatttttt tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac
     4441 tcagctaatt ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg
     4501 gcaccttctg tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa
     4561 caacctaaaa tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa
     4621 aacagtaaaa tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa
     4681 acggaacgca gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca
     4741 atcagtaata aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca
     4801 tccacagcat tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc
     4861 tttagtccaa ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa
     4921 ataaagtaac cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc
     4981 agcaaaatat agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata
     5041 tttgttgcaa aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat
     5101 caattaatta tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag
     5161 acgctcaacc aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg
     5221 ttcttctgga tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc
     5281 cccttcactt tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa
     5341 aacaccatca cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata
     5401 aggaccgtat ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc
     5461 cttatcgatc aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt
     5521 atctgggtaa tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt
     5581 tcttataaaa acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct
     5641 cgataaaaat taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact
     5701 tgccagatat aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca
     5761 gtgatctctt ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact
     5821 acttttgcat tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt
     5881 atccctcgtg ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca
     5941 tgtttggcaa ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat
     6001 gtttcgaata aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat
     6061 acctcttctg gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact
     6121 acattgatgt ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca
     6181 ggaattcccc atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca
     6241 ggacaatttt gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt
     6301 gtaacgatta aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta
     6361 cgtaaattag gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc
     6421 atcatgccat tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc
     6481 gttccttcat gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt
     6541 ccaggatcca ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat
     6601 gtttctacta accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc
     6661 cccccatatc gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa
     6721 cccttttata tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc
     6781 agtataataa agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat
     6841 caagatgtcc ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt
     6901 caaaaagaaa ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct
     6961 gaatcccttg atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa
     7021 aacgagatca tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt
     7081 gatcaatctg gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt
     7141 aacgattatg atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt
     7201 cttcaatcta accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac
     7261 aaagataatt gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta
     7321 gtgggcgaca tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc
     7381 tgtagcggag tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc
     7441 aacgaaatga tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa
     7501 atcggactga catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact
     7561 gataaatttt gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt
     7621 caagtagatt cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg
     7681 aataacaaat ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca
     7741 agatgtatcc atccaatcga taccatcata atctattgct tcttttataa ccgttatatg
     7801 ttgcctaaat aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc
     7861 ttttttcgaa taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg
     7921 tcgttttcaa tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg
     7981 tccatatatc tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca
     8041 taaagcaagt attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc
     8101 tcacataatt ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca
     8161 taatatacaa tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta
     8221 cgctccacat aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg
     8281 tttgcagctg cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta
     8341 aaataaaata atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa
     8401 aataaaattt gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa
     8461 attttataag attcttggta ttttgataca gaattcaatg attgaatctc atttattttt
     8521 ttcctgattt tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt
     8581 ttgcaaagcg cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg
     8641 acagttgctt gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt
     8701 cggtattctt ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaa
//
LOCUS       DAJSBC010000097         8673 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSBC010000097 DAJSBC010000000
VERSION     DAJSBC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.099"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..834)
                     /locus_tag="ONT65_002475"
     CDS             complement(<1..834)
                     /locus_tag="ONT65_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2330065.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GI"
     gene            980..1783
                     /locus_tag="ONT65_002476"
     CDS             980..1783
                     /locus_tag="ONT65_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR2330066.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1961..2908
                     /locus_tag="ONT65_002477"
     CDS             1961..2908
                     /locus_tag="ONT65_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR2330067.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2976..3269)
                     /locus_tag="ONT65_002478"
     CDS             complement(2976..3269)
                     /locus_tag="ONT65_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR2330068.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3297..3962)
                     /locus_tag="ONT65_002479"
     CDS             complement(3297..3962)
                     /locus_tag="ONT65_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR2330069.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4102..4734)
                     /locus_tag="ONT65_002480"
     CDS             complement(4102..4734)
                     /locus_tag="ONT65_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2330070.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4741..5808)
                     /locus_tag="ONT65_002481"
     CDS             complement(4741..5808)
                     /locus_tag="ONT65_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2330071.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5805..6536)
                     /locus_tag="ONT65_002482"
     CDS             complement(5805..6536)
                     /locus_tag="ONT65_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR2330072.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6705..7184)
                     /gene="greA"
                     /locus_tag="ONT65_002483"
     CDS             complement(6705..7184)
                     /gene="greA"
                     /locus_tag="ONT65_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR2330073.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7320..8495)
                     /gene="mltG"
                     /locus_tag="ONT65_002484"
     CDS             complement(7320..8495)
                     /gene="mltG"
                     /locus_tag="ONT65_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR2330074.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2587 a         1775 c         1340 g         2971 t
ORIGIN      
        1 aataccatat tttaatttta ataaagaaat tatacgagga tatatctttt caatctcgct
       61 tagtagctga tcttcaaaat caatatgtcc gtcgaaaata attatctttt cgctatactg
      121 ccaaatatgg acatgatgaa catcctttac cccatcgatt gcttcaattt cttgttccat
      181 tttttcatag tcgatagcag gtgcactttg tatcaaaata gcggcagctt ttttgatcac
      241 cattatcgct tctatcaata tatatatcga aatgatcagt gtaataactg gatcgatcca
      301 gtagatatcg aaaaaacgga tcaaaattgc tccgataatc acacttaccg aagacagtgt
      361 atcacttaac aaatgcaaat aactagattt tatattcaaa ctttcatctg cttcttgtcg
      421 taataataat actgagaaca aattcgcgag caatccaata actgctactg ttagcatcaa
      481 atgactattt atcttttctg gtgaagtgaa ccgcttgaat gcttcaacgg ttaaaaacaa
      541 ggataatatg atcaatatga atgagttgat gaaagcactc aatatttcta aacgctggta
      601 gccatacgta cgttttttat ttgcttcttt tcgggccatt ttttgtgccc cgtaagaaaa
      661 aataattgcc aatgtatcac taaggttatg gaaagcatct gaaacaagag acaaactccc
      721 tgacataatc cctccaataa attctgataa agtgatcacc gcattcaaaa ccactactcc
      781 aaatagtttt tttgatgatc tctgtggctg tgtaggcact attccttcct ccattttatt
      841 tatattggat agtctttaga caagaataac atttttttga tatttcacca atcattcagc
      901 tgtttcctta aagcacttgt atttcacttg atacttcgct agaattgcaa gtgatgacac
      961 tactagatga ggagtactta tgacaacaaa acaattaatt atcagttcct ttatacgatt
     1021 caaagaccta aaggaggcga gaaatgtccc attttggaaa gccattgtct atcttttagt
     1081 tttgagtata attatggctt tgccaatctc ttttcaagtt tttcaagtct taaacaacat
     1141 tcaattggat gggcaaaaga ttgctactcg cattcctgat tttacaatcc aaaacgggca
     1201 gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca aactccatta tcttcacatt
     1261 tgatccagag ggaaaacgaa cagaacagga catctctact gatttgatgg ggaattttgt
     1321 tagtgtcggg atgttaaaaa acaaacttat catagctctt cctaatacag gcacgacttc
     1381 tgctttgttg aacaataacc aattagaact tccctacacc aatgaatctt taaaaaactt
     1441 aacaggtaag cagctccgtt cttttttaag tgaagcatct ataccacttt ggataaaagt
     1501 cctgacattt atattttcta tttatcctag ttttctgaat ctgctgatca ctttgctatt
     1561 taccaacatt gctgcttttc tctatgcacg ctttcgacta acaaaagcta cttttcttga
     1621 ttgtttaaaa acattgatct attcaatttc attgcctact ataattgcaa caatcttgat
     1681 ggtctttctt ccttcttttg acactagcgc cttcattgct tttgcaggaa tctttatttt
     1741 tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat taagaaataa agacaactcg
     1801 ttatttctac ttcaatagac ccttttagag gttgtgacat ttttttgtca cagccccttt
     1861 tttatttgaa aaatatttct ttctcaaaaa acaagcaagg cgcttcaaag ttcttcttgt
     1921 atgcgctaca cttagagtaa agaaaggcgg gaaattaact atgtcagaaa gaatcattta
     1981 tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc catactatag cgcctggtta
     2041 tatctttaaa acagaattac aaccagagga tctacaaaat gtagagatca gtttgggctg
     2101 gaactcagac taccaagaaa aactattagc ctcttctacc ttgaaatggg tccaatctat
     2161 ctcagctggt gttgatcagc taccccttga gactttttca aagaaacata ttttactttc
     2221 aaatggtagt ggcatccaca gtgaatcgat tacggagcat gtcttaggaa tcatccttgg
     2281 gtattctcgt ggtctttttc aagcacaaaa ggcacagagt gacatgaaat ggttaggttc
     2341 atcggtccat taccaatcta tccaagggaa aaaaatacta atcattggaa ctggccatat
     2401 tggaaaaatg attgcaaaaa aatgcaaagc attagacatg aactgctatg gaatcaatac
     2461 atccggtcat ccagcagaag gaatggaaaa gactttttct ttaaatcaac taaaggaagt
     2521 tcttcctgaa gtagatttcg tagtcaatat cttacctcta acggaggaaa caactgggct
     2581 ctttgatcag catatgtttg aacactttga tccaaaatct gtattcatca atgttggccg
     2641 tggtgcttct gttaagacac aagatctggt gcaggcttta aataaccagc aactttcctt
     2701 tgcagcatta gacgtttttg aggaagagcc tcttccacaa gatcatcctc tttggaaaat
     2761 ggacaatgta ctgattactt cccacattgc aggtttgaca cctgattttc aaaagaaatt
     2821 aatggctatt tttttaacca atctgaaaag ctactttgcc acccatgagc ttcaaaccaa
     2881 tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca atattttcaa aagaaaaaag
     2941 agaaggtgac aaaaattgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt
     3001 ggctcctcca ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca
     3061 tctgcttcct cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct
     3121 ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact
     3181 tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt
     3241 tgtgcttttg gcgttaccgt taatttcatc ccaacaacct actttctctt ttctttctat
     3301 tgattcatct tatcgtctag aatatccaca tcgcctgtct ctccaataac cagtagatta
     3361 tcattttcac gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt
     3421 cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg
     3481 ttgaaaaatt taggattgga gactcggatt tctgctaaag aatatttatc tgacaactct
     3541 aggtaatcta aaatattctt tgacactaaa ttatgcgcaa tccttattcc catgtctcgc
     3601 tcgggatgga cgacgcgatc agctccgatc ttttcaagta ctctggcatg atactcattt
     3661 tgggcttttg ctaaaatatt tggtacgccc atttccttta ccatcagagt aactaagata
     3721 ctggcttgaa tatcttctcc aatagcgaca attacatgat cgaaatttct tatgcctaat
     3781 gatcgaagtg tcatttcatc ctgtgcattt gccacaacag catgagtggc tatattcata
     3841 tattcattga cccgatcttc attactatca atagccagta cttcttgacc agcttcgatc
     3901 agtgttcggc agatacttcc gccaaatcgt ccaagaccta caattgcaaa attttgtttc
     3961 accgttttcc tcctttagta tcttcttttc tcagtataac aaagccttta caaaaacaaa
     4021 agcaagcatc attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga
     4081 ttatcctctt tatcatcgtt tttatttcac caaaccatgt ttgaatgcat agatagcggc
     4141 ttgtgtacgg tcttccactt ctagcttagc caatatattg gaaacatgtg tttttactgt
     4201 tttcagtgta ataaacaatt cgtccgctat ttcctgatta ctttttcctt cagaaatcag
     4261 cattaaaatt tcattttccc gattcgttag ttcttcatgt aatacaggct catttcgccg
     4321 actcattttt tccatcattt ttgttgtcac ttctggttct aatactcgct cgccacgttg
     4381 cgttgccctt atagcatcag caatttcatg tgcggtagag gtcttcaaca aataccctgc
     4441 tgcacctgct tcaatggcag gataaacttt ttcgtcatca ataaaactgg ttacgataat
     4501 gattttagct tctggccaat cttttaagat tgcttttgtt gattcgatac catccatttc
     4561 ttccatgact aagtccatta aaatgacgtc cggtcgaagt gtcatcgcct tctcatagcc
     4621 ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct tcttgaatag ataaataaga
     4681 agagacgcct aagcgcacca tttcatggtc atctactaat aaaactttta tcatccttat
     4741 ctatgcctcc ttcatcaaag ggaccttgat ttctacactt gtaccttgac ctttaaaact
     4801 aatgattttt actgttccac caataccagc tactcgttct ttgatattgt tcaatccata
     4861 acttcccgtt ttcgtttcat tcatattaaa ccctgttccg tcgtcaataa tacgtaacaa
     4921 aagattattg tctattttgt gcaaatatac ctctaattca ttggctttag catgtcttaa
     4981 tgtatttgat aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagatag
     5041 ttttacatct tctacatccc atttcagcga aatctggatt ttattttgca attccttcaa
     5101 taactgctcg ataccttgct ttaaactttt ctcttctaaa ttgactggac gtagatgaag
     5161 aagcagcgca cgcatctcag actgggaagc gttgatgatc tctgctacca ttttcaactg
     5221 cttgcgaaac atttctggtg tctcgctttt ttctgcttgc tccgtcaatg ccgacatcat
     5281 catcattgct gcaaacaatt gttgcgaaac tgaatcatgc agctcacggg ctaatcgatg
     5341 tctttctaat tctaaaattt cttctttggt ttgtccatct acatattggg gacggctcgt
     5401 tacaatttgt aattcactag atacttcaat cattttttct tttatcttag tgatttcctt
     5461 atcaatatct ttgatgtata aatcgtccga tgcacttggg atcggaagaa gcaacaattt
     5521 ggattcataa ttgccagaag aaagtgcacg cagcttttct tcgattttcc cgtattttgt
     5581 cttttgaata agtgataata atagaaagac aattaatcct acacctaagg aaatggctaa
     5641 caaatggaag ataagcggaa catataataa acgtgcacgc agtaattctc cccaccaatg
     5701 actttgattg ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc
     5761 aatccccgaa taaacagcta gcatcgctct ggatattttt cccatcatat tcgtatcacc
     5821 tcgacatctc caatcaaggt attagtaata atcttcaatc tacgagggtt ttcatcataa
     5881 tcactgctgt agattttgat ttgttcattt ttcaaagaaa attgctcttc ttcaaacaag
     5941 acatttccta ctaatgtcgc atgctctaat cggatggcta cgcccaaagg tactaagatc
     6001 cgtgttctgc ctatcccttt tcttacaatc acaatattat catcttttgg caaaagcgta
     6061 ttgcccaaat ctatgatagt atcaccagaa atgacggcga tgttgatatc gtcccattca
     6121 tagacgtcgt ttccaatacg ttggttaccg aataattgct gtcttttccg ctcattatta
     6181 tgtgttttta attgctctgt ttggatcatc atgatctgtt tctttcgcca aaaagcattt
     6241 ttggttaaat ctatccctga aatctctaca cctttcagac caataaataa aacggcaaat
     6301 acagccatca tccataaagc aggactgttg actaagctaa aaaaaatgac caaacttcct
     6361 aatatcaatt gaaaattttg aaatttagta cgaggatgtt tgcgcatagc tagatatata
     6421 ttgaaaatac cgaatatcac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca
     6481 gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac gccaagaact attcatcgct
     6541 ctttctccct cctgacacat tctcatttca tcatacctat ttttttaact gttggctata
     6601 ggactttaga tgtaagatcg tttgttgaaa tctaaaatac acaaagagaa aaagatctag
     6661 agtgtatcat cagtaagatg aactctctag atctttcagt cagactacgc ttgttctact
     6721 ttgacgattt taactaacat atcgccacct ggagtagcaa tagttacttc atcattttta
     6781 cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg aaattttgcc agaaaacgga
     6841 tctgcttcag cgcttccgac aatcgtgtac tcttcttctt ctccatctgg tagttcgatg
     6901 aatgtaactg ttttaccaat tgatacctca tctgcatcta caccgtcgtt atcgatgatt
     6961 tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc cttcaacaaa tgcttgttca
     7021 tctttcgctg attcatattc agagttttcg gaaagatctc cgaaaccgcg ggcaattttg
     7081 attcgttcaa tgatttctcc gcgtttgact gtttttagtt cttctaattc tttttctaat
     7141 ttttctttac cttctagagt cattggataa actttctcta ccatgtgtaa cgtctccttt
     7201 tttcgataaa ataccgcaaa aggtaatctt ttgaggaata tagtccaaaa aagattacca
     7261 cgcttttctt caaatgtaaa tgtttttcta aaaatgatca atgtctagag cctgtttttc
     7321 taagaattat ttacatattt ttccactaat tccatatgtt cttcataagt tttagcgaaa
     7381 taaacatttc cagtgctcgt atctgctacg aaataataat agtcattttc tgctggttct
     7441 aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag gtccaggtcc atagccggta
     7501 ttggtataca aattatatgg tgaatctaca gctgtatctt catacgtcac taattctttg
     7561 tgttcaccta atgcgtataa aacagagata tctgactgaa gtggcatatt tgctttgatt
     7621 cggttaaaga agacttgtgc aatgttttta cgatcatttt cttttacgcc ttctttttca
     7681 actagagaag ctagagttaa aacttcttgt acagtcaaat ttttctgctt gatagctgaa
     7741 taatagtttt gcatgaccgt attcgtcttg ttcaccattt caatcactag atccttaagc
     7801 gaagtactcg tgtaataatc atatgtggct gggaataagt aaccttccaa acggtatttt
     7861 gtgttctcag ctttagatgc actttccaac agttctggga atttttggtg caactcgtta
     7921 aagaaagtct catcattcac taaatctaaa aattctttct tgtctttacc agttaccttc
     7981 gctacccgtt cagcaatctg atcgatatcg tacccttctg gaatggcaat tttcccatct
     8041 gcaacttttg ttggctcaga agtaccgcct tcttgcaact gtttgccaat ctcgtcaaga
     8101 gtcatattag gcgctaactg ataatagcca gcttgaaagc cagttaaatt tttaaatttt
     8161 gtataataat tgaaaacaat cccgctttta ataatattgt ccttctccaa aatctcacca
     8221 atctgcttgt tagaagagcc actcgggatc tcgacttgga ctaattggtc atctgattta
     8281 tctaaaggct tcaacccgct atctacataa cgatacacgg taaatccaag aaatccgcca
     8341 ataatcaata atgtaagagc aatcactaaa atgatcttcc tcacaatctt atcttctttt
     8401 ttacgcagat ttctccgctt gtcgattata tctgctgtag ctgccttact ggcttctttt
     8461 ttatctttac ttgggtgttg tttgtttcct gacaaaacaa ctcctcctta ctatcaagtt
     8521 cgtaacttta ttcattatac atgatatttg aattggcgtg aatgatttta tttaaatatt
     8581 ctatcaaaaa tgtaagtagt aaatccatta ctataagatt aaaataaatg ttatcaagat
     8641 acaagctatt ttagctttat tctttctgta aaa
//
LOCUS       DAJSBC010000098         8489 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSBC010000098 DAJSBC010000000
VERSION     DAJSBC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.100"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..173)
                     /locus_tag="ONT65_002485"
     CDS             complement(<1..173)
                     /locus_tag="ONT65_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2330075.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="ONT65_002486"
     CDS             complement(593..1714)
                     /locus_tag="ONT65_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR2330076.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ONT65_002487"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ONT65_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR2330077.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ONT65_002488"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ONT65_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR2330078.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ONT65_002489"
     CDS             complement(4304..5215)
                     /locus_tag="ONT65_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2330079.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ONT65_002490"
     CDS             complement(5232..6239)
                     /locus_tag="ONT65_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2330080.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ONT65_002491"
     CDS             complement(6236..8365)
                     /locus_tag="ONT65_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2330081.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8489)
                     /locus_tag="ONT65_002492"
     CDS             complement(8370..>8489)
                     /locus_tag="ONT65_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2330082.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1485 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgacc
//
LOCUS       DAJSBC010000099         8328 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSBC010000099 DAJSBC010000000
VERSION     DAJSBC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.101"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..1020)
                     /locus_tag="ONT65_002493"
     CDS             complement(61..1020)
                     /locus_tag="ONT65_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2330083.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1116..1661
                     /locus_tag="ONT65_002494"
                     /pseudo
     CDS             1116..1661
                     /locus_tag="ONT65_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1844..4519
                     /locus_tag="ONT65_002495"
     CDS             1844..4519
                     /locus_tag="ONT65_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCR2330084.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4701..4940
                     /locus_tag="ONT65_002496"
     CDS             4701..4940
                     /locus_tag="ONT65_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330085.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4956..6329
                     /locus_tag="ONT65_002497"
     CDS             4956..6329
                     /locus_tag="ONT65_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330086.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            6333..7967
                     /locus_tag="ONT65_002498"
     CDS             6333..7967
                     /locus_tag="ONT65_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330087.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            8130..>8328
                     /locus_tag="ONT65_002499"
     CDS             8130..>8328
                     /locus_tag="ONT65_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330088.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2874 a         1338 c         1644 g         2472 t
ORIGIN      
        1 tgaaaatcac ctttataaac tccattcttt tccactgttt tattaatatt ttctgatttg
       61 tcaaattaag ctagacaata tgtctttatc tatgcagctc aaaaatactt ccaatggtgt
      121 tcgataattt aatgattttc ttggaatatg gtttcttttc gaggcaacgg attgaataaa
      181 ggtttcatcc gtgtccctaa agtccattgt tttaggtaag ccatctcgtc taagcaagcc
      241 gtttgagtgc tcatttagcc ctctttgcga aggtgttcct ggatctgcga aaaagatggc
      301 aatatcgttc gagttactta tagatcgcca gttggaaaat tcttttccac aatcaaatgt
      361 catggattta aacaagtgtt taggcatgga tttgaaccaa tcatttaacc tgttttcgat
      421 atcttcggct cgtctgcctg tcggtttcaa agtaataata accttggata aacactcaac
      481 caatgtaata acagcgctct tgtgtttaac tcccacaatg gtgtcgactt cgagatgacc
      541 aaattcttta gtcaggtgtg gatagagact gttccgttca tgaatcgttc tgcgaaaggc
      601 ctgttttcca cgcttttctt gatgattatt cggtttacgt ttccccttca taggtaacga
      661 ctgtgtgtca aaaaggcctt ttttaaacat ccgatagagt gtacgaacag aacaatcaat
      721 tggaaactcg ccacggccga taatcacatc gggtgaccat ccttgagcta ccttttcttg
      781 aacatatgtt gattgttcat cagataaaac ggttgaacgt cgtccacaac gagatttatt
      841 ttgtttgtat tgacggtaat aatctagcac tttttttcct tgtttgagaa acgaataaac
      901 attataaatg gtttgtctag agcgatttaa actttgtgcg acaagagcga caggtttctc
      961 gatattgaaa taagactcta tgattactag ttcgtctgtt gtaagatggg tgtaagtcat
     1021 ttgtactcac tccttattaa ttctttggtc ggaactattt tgagtgtagc acaaatgatc
     1081 tttttagttg tctagcttaa ttttacaatc gacgttttca aaatgctgag actattgaac
     1141 gagaacatca ttatttcgat ttattagtga atacagtggt agaattagcg aaatgggcaa
     1201 agtcaaacga aacttatagt caagtactta aaagaattat gagtcaagaa acttcattta
     1261 tctatgagtt aagcaagtca aatttgaaat atacttataa tgaagatgaa cttgaaattt
     1321 tagataatcc agaaaattca caatggatta aagagttgaa gagagaagac tttattgaga
     1381 cactaagaat tctaaaatcg ttatttgatt tgaaattagc caaagctacc atcatgggaa
     1441 ttgaaagaga ttcatattat tgggaggaaa ttggactata tgctccccag attgaaggtg
     1501 aagataaaga gaaaaggata gacaacatta ttaactatat taatagttta agtgaacctt
     1561 taaaatgtag aggacttgat tttataggaa cattaggatg gacagaaaca tactatgaga
     1621 aaatatataa agaaataatt gaacaatatt ttcctaaata atttaataag gattgattta
     1681 tcgtaaaaga aaaaagttgt gatttccaaa atcacaactt ttttcttttg tcaagatttt
     1741 gagtgggata tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg
     1801 tagtcataca aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac
     1861 ttcaattcag aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag
     1921 aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca
     1981 agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta
     2041 taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat
     2101 tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac
     2161 agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga
     2221 aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc
     2281 cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt
     2341 acttcaccga agatttggag tagaagatga agttgatatt gatacttctg tttataatcc
     2401 ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg
     2461 tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt
     2521 taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag
     2581 ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc
     2641 tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact
     2701 tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc
     2761 tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga
     2821 tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc
     2881 attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt
     2941 tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg
     3001 caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg
     3061 ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa
     3121 acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca
     3181 acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga
     3241 agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa
     3301 aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca
     3361 taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt
     3421 gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca
     3481 gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa
     3541 tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc
     3601 aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa
     3661 ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa
     3721 ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg
     3781 tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc
     3841 tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa
     3901 tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag ggattacaaa
     3961 aattgaagca acttttgtcg aagctcaaaa aaaactatgg gctgttttac ctgaacattc
     4021 tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagccc tcctaattca
     4081 agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta
     4141 cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa
     4201 aacattgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc
     4261 cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt
     4321 tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct
     4381 ttttaggcaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg
     4441 tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa
     4501 atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat
     4561 ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg atagcgagca
     4621 aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca
     4681 tgaaagaaag gaaaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc
     4741 tgtatattca agaacatttt tcaaatcaag ccttttttac aaaaccaatt tacttggatt
     4801 tcgaaataag aggggtatct gctggatcta tcgggggtac tcttcaagcg ttgaagaacg
     4861 aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc gtgaaaaaat
     4921 ggtatttagt tcattcatag agaggggaat caagcatgcg tgtggcttgt tatgatcgaa
     4981 cgtctgttta tgaagaagcg aaaattagta atgacgctca atatgatgca gtgaaagagt
     5041 atgttttaga gaaatatgaa caaattcccc aacgattttc agacaaagga gaatcagggg
     5101 aaaaagcgga gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag
     5161 agggggtgat ctatgtttgg cgttatgatc gattggcacg tcaagccaaa atagccttag
     5221 aatttattga gttgtgccaa caacatcagg ttcaagtgta ttcgatttct gaacctcttc
     5281 ctgcttcttt ctcacacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg
     5341 cagaacaaca acgaaacgtc attattgaga atgttcgtgc cggactttcc tataaaaagc
     5401 aacaacggca atatttgtct tctgcggtac catatgggta tcgtttagtg aatgggaaaa
     5461 tccaacaaga agtacacgaa gcaaggattg ttcgctatat ttttcaactt tatttgacca
     5521 aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagctt ttcttccgcg
     5581 aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg
     5641 aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaag
     5701 caaccttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt
     5761 atctgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga
     5821 agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa
     5881 gctgccaagg aatttttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac
     5941 aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaatta acgcaacaat
     6001 tagccgaaaa gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa caagaagcac
     6061 aatggattaa ggcatttgaa gagggcaatt gttcactgga agaactcaag caacaactgc
     6121 aagctcttca aaagccgatg tctacttctt cgtttctttc actccaacag tgtgaagcaa
     6181 aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattatta tgtgactatg
     6241 tttgtcggat tgaggtaaca aaggataaaa cagtttgtgg gatttacttc cgacaactga
     6301 cacaaaatat tttagaaagg aacgaatgat ctatggaaat gatacaaacc aaacgagtcg
     6361 cattgtatat tcgcgtgagt acacaagaac aagctttaga aggacatagt attcaagcac
     6421 aagaacaaaa tggacgacag tttgccgaac ggatgggcta tgaagtggta gaagtttatg
     6481 ccgatgaagg aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga
     6541 acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac
     6601 gcaatatgtt agatgtctta caaacagtcg aggaattatt gcgatatgac gtaggattac
     6661 attcgatctc ggaacaattt gacgtgacca cttcgacagg taaattgatg ttacaactcc
     6721 ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa atgtcaatgc
     6781 aaagcttagt gaaagataaa aagcgttatg caggaggccg acgtttaggc tatgtcagtg
     6841 gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc
     6901 gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga
     6961 atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata
     7021 tcttacgcaa taaaatttat gcagggtatt tagaatatgc gcgctacatc aattgggaaa
     7081 ccaaacgacg gaaggggaag aatccgagac cgattttagt gaaaggaacc catgaaccga
     7141 ttatttcgga agaagattat caaaaagtac aagaacgcct tgctttagaa ggaaaacaac
     7201 ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg
     7261 gcgcaccgat ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca aagaaacgga
     7321 ttcgatatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt
     7381 cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc
     7441 taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc
     7501 aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa
     7561 ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc
     7621 gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag
     7681 ggatctttca acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactaatt
     7741 tggatcgttt tcttgcccag agtgagaaga agcaaatcaa agccttgtat cgtaccttta
     7801 tagagaaaat aacctttgat cctaaccata aggagatgtt ggaaattacg atgaaattca
     7861 cgcatgccgt ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg
     7921 atacggctat ttttgtgtta aaaacaccct ttacgttaac gatctagtct aggttatatc
     7981 aaacaacgtt agaggggcgc taacgcttct ctagcgattt gaaatcgtta gagaagtagt
     8041 tagggaggtc cttatcataa atagggatat ttattaatga atatttgaaa tataattgta
     8101 tataagaatt aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg
     8161 aaaaatacac tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag
     8221 gtaaaaagac cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg
     8281 attttccgaa ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DAJSBC010000100         8040 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSBC010000100 DAJSBC010000000
VERSION     DAJSBC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.102"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            301..2067
                     /locus_tag="ONT65_002500"
     CDS             301..2067
                     /locus_tag="ONT65_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR2330089.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2169..3428
                     /locus_tag="ONT65_002501"
     CDS             2169..3428
                     /locus_tag="ONT65_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2330090.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3575..4876
                     /locus_tag="ONT65_002502"
     CDS             3575..4876
                     /locus_tag="ONT65_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2330091.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4879..5730
                     /locus_tag="ONT65_002503"
     CDS             4879..5730
                     /locus_tag="ONT65_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2330092.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5743..6558
                     /locus_tag="ONT65_002504"
     CDS             5743..6558
                     /locus_tag="ONT65_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR2330093.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6709..7875
                     /locus_tag="ONT65_002505"
     CDS             6709..7875
                     /locus_tag="ONT65_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2330094.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2622 a         1384 c         1596 g         2438 t
ORIGIN      
        1 aggctagtct aaattattat gtttcattct tctagaatat acttctacgc caaaaactgt
       61 taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac
      121 taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct atttggcaag
      181 tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg
      241 tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca
      301 atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat
      361 acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta
      421 tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa
      481 aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagt
      541 cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat
      601 caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata
      661 ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg
      721 tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact
      781 ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga tttgcaaggt
      841 gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta tttttgtccc
      901 atatttaaag ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc
      961 gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata
     1021 cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac
     1081 gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca
     1141 gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact
     1201 tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat
     1261 ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat
     1321 gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc
     1381 aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg
     1441 gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga ttattttgca
     1501 aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac
     1561 agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc
     1621 ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt
     1681 caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac
     1741 ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt
     1801 tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct
     1861 actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg gaaactagag
     1921 ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa
     1981 aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga
     2041 caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg
     2101 caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat acaaaggagg
     2161 aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa
     2221 tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg
     2281 aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa
     2341 aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg
     2401 atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg
     2461 cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg
     2521 gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct
     2581 acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata
     2641 cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg
     2701 agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct
     2761 atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag
     2821 atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca
     2881 aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc
     2941 aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca
     3001 aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt
     3061 atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc
     3121 tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa
     3181 tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg
     3241 aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag
     3301 aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa
     3361 aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa
     3421 aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata
     3481 aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag
     3541 tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat
     3601 caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg
     3661 ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct
     3721 tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga
     3781 tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt
     3841 gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg
     3901 caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc
     3961 ttatctattg acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct
     4021 agttacattg ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt
     4081 aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa
     4141 tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca
     4201 aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg
     4261 tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag
     4321 tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat
     4381 aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac
     4441 gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt
     4501 tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc
     4561 aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc
     4621 aacatttatt acacagtata cggggaattt caataatttc tccatgatct atctgtttaa
     4681 caatggcggt cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg
     4741 gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt
     4801 gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt
     4861 caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt
     4921 tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt
     4981 ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt
     5041 tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact ttacgggact
     5101 tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta
     5161 actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg
     5221 ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg
     5281 gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt
     5341 ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta
     5401 gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta
     5461 ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg
     5521 gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc
     5581 ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca
     5641 attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc
     5701 ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta
     5761 aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca
     5821 tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact
     5881 cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg
     5941 ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta
     6001 caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct
     6061 gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc
     6121 caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa
     6181 ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc
     6241 agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc
     6301 atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa
     6361 ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata
     6421 ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt
     6481 gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa
     6541 gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg
     6601 aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt
     6661 cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa
     6721 actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg
     6781 gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc
     6841 catgaagaaa tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc
     6901 tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat
     6961 cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa
     7021 aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac
     7081 ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt caatacacct
     7141 gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt
     7201 atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact
     7261 gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat
     7321 ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg
     7381 gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa
     7441 catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca
     7501 aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg
     7561 atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat
     7621 attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg
     7681 acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg
     7741 ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa
     7801 tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca
     7861 atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag
     7921 ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt
     7981 agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac
//
LOCUS       DAJSBC010000101         8032 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSBC010000101 DAJSBC010000000
VERSION     DAJSBC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.103"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1324)
                     /locus_tag="ONT65_002506"
     CDS             complement(<1..1324)
                     /locus_tag="ONT65_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2330095.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRII"
     gene            complement(1335..2003)
                     /locus_tag="ONT65_002507"
     CDS             complement(1335..2003)
                     /locus_tag="ONT65_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2330096.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2133..2717)
                     /locus_tag="ONT65_002508"
     CDS             complement(2133..2717)
                     /locus_tag="ONT65_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2330097.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3048..3281)
                     /locus_tag="ONT65_002509"
     CDS             complement(3048..3281)
                     /locus_tag="ONT65_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR2330098.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3296..4246)
                     /locus_tag="ONT65_002510"
     CDS             complement(3296..4246)
                     /locus_tag="ONT65_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2330099.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4243..5085)
                     /locus_tag="ONT65_002511"
     CDS             complement(4243..5085)
                     /locus_tag="ONT65_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2330100.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5087..5806)
                     /locus_tag="ONT65_002512"
     CDS             complement(5087..5806)
                     /locus_tag="ONT65_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2330101.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6498..6656
                     /locus_tag="ONT65_002513"
     CDS             6498..6656
                     /locus_tag="ONT65_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330102.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6728..7954
                     /locus_tag="ONT65_002514"
     CDS             6728..7954
                     /locus_tag="ONT65_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2330103.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
BASE COUNT         2826 a         1370 c         1063 g         2773 t
ORIGIN      
        1 aaataattct attgcatgtt ttataaagaa attccggatc atgagtagct ataactactg
       61 cacccttttc ttttaagtct ttaataaggt cagcaatcct ttccatatta atataatcca
      121 acccacttgt aggttcatca aatagaaata aattagtttt tgacaatgaa gctatagaaa
      181 ccaataatct ttgtttttct ccaccggata aatcaaacgg taattgatct ttcattttat
      241 aaagttccat tttttttaaa attttttctt gctgatcttt actaactatt tcattcccca
      301 gggctaactc gctcctcact gattctgtga aaaattggta atccatttct tgcattacta
      361 aaaacggcga tgacaaattt tttttatttt cgattatctt tcctttggta gggcgaataa
      421 tcttcatgag tattcttagt aaggttgttt tccccacacc gtttttccct accaaaccaa
      481 taacatctcc aactttgaca tcaaaattta tgtcttttag aatattttca taagtaattt
      541 cttcaacttt taatacatct gcacttacct tttcgatata gggaacttca atcgaactat
      601 taaaaatgtc aaatttcctt aatgcgttat tgttttcagt ttttaacaac tgaatagttg
      661 tagtttttac agttctattt ttaacaaata gaacttgatc aattaattct ttcaaatagt
      721 aaaaacgatg ttcagctata acaatagtaa atcctttatt ttttaaatca caaagtaagt
      781 cagcaagttt cttagtcgct ttgaagtcca aatttgaaga cggttcatcc aatataatta
      841 atttagagtt tagcgataaa ctacatccaa tagaaatttt ctgccgttca ccacttgata
      901 gcgaaaaaat gtttttatct aatatatttg aaagatctaa taattcagct aattcattga
      961 ttcttttatc catatgctct tttgtaaatc caaagttttc catcgaaaat actaactcac
     1021 tcgttgtatt tgttgtaaag aattgtcctc taggatcttg acttacgtag ccaatatttt
     1081 ttgatatact ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa atttccccaa
     1141 ccaattttcc gtcataaagt cttggaataa gtccattcat taactttaat agtgtggatt
     1201 tcccactccc agaagaacca gtaatcaatg taagactgcc tttttttatt gaaaaattaa
     1261 ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa agaaacattg ttaaattcaa
     1321 acaattcttt cacctcaaat caaaatactt aagaaaaaaa tagtaacgga aaacaggatg
     1381 atatagtcta ataagctcat gtttgtttta tagtaagaac ttcttttcac aggagattct
     1441 atcccctttg ttattgcatt agcggtcatt tcttcagcaa tcatagttgc acgtataaga
     1501 acgggaacta acagaaattc caaatattgc agaggctgaa aaaaagacaa gcgaatgttt
     1561 ctcattttta aagattcttt aatcattctt atttcagaac gataagtcgg aaagaatctg
     1621 atcactactg taatacttaa cattattttt ttcggtaaat gtattttttc taaagaggat
     1681 aataattcat tgctagtata gacactaacc aacaaccatg agatcattac gaccggcaac
     1741 attcgaaata caatatataa ggatgtcgca aaaaatatag taaaaaactc tatcttgata
     1801 gcatcaataa ttttcattac taaaaacaga actgaataaa tcaagatgaa ataaatacat
     1861 gtctttattt tacgaaacat caatactaaa actgtagcaa taatgaaaca ccccacttga
     1921 aatatatttc cctttcctaa aaaaataatg accggaaaaa aaatatttaa agtaatcaag
     1981 gttaacggat tgatttttga cactctttca cctccaaaaa ataagatcgc actgttatct
     2041 taagctgata aaaatgcacc gttattcaga taacagtgcg atcttatcaa aaagcataac
     2101 tgagaacaat tctcaatgca agcttttttt aattaactca gtaattcaat ccacccagga
     2161 acagaaaatt gatacaatct tctggttaat agcatagtct tttcttttga atagctatgc
     2221 ttcaatatat catagaatgt agcgtagaat gagtaaatgt agaagtggat ttcttcttca
     2281 ttaaagacta aattttgatt gatcattccg atagtcttta acttatctag acaacagact
     2341 gtctttttcg tcacaaatga tgtaattttt tcaacaatat cttgatattt ggttccagct
     2401 gattggaata aaatcaaatc gaatatacgt ttattctcaa aaatataatt aataaatgga
     2461 tctaattcat tgaagttctc tctccaactt ttctctatat ctatattttc taagtttaag
     2521 aggtatttgg taaactcttc attatacata ttaataagcg cattaatatg cggctgaata
     2581 atttcttcaa ataacgcttc cttagaagta aaatgtttat aaaaagcacc cgtagttacg
     2641 cctgcggtca ggcacagttg ccttatctta gtttgctgaa atccttcttt ttcaaaatgg
     2701 cttagtccac tttctattaa cttttggtga gttataatat aatctctggt ttctatgggt
     2761 acttcctcct aataagatga attaacttta ttatcttaca caggacttga agtttcaatc
     2821 gttaacgttt attactgaac tcattcctta gagctaaact aatgagaaag agcagaataa
     2881 ctgatacaaa aagcaatcct acaagaatta atagactcat ctaaatcatt caccttttct
     2941 aaaataatcc gagaaagatt taaaaaaatt atatccagct attaggataa gtcctatcat
     3001 aatcactcca atagccattt tcttatcctc cctaaaaata aagtctttta cttaaaacct
     3061 tttttgtaat cgtcaataaa ttttttccct ttatcagtta attgaaatcc tatatctaaa
     3121 tagtcctcat atctaagttt agccagcatc tcacttacag atgctggact aacacttaga
     3181 acttttgcta aatcattatt ttttaatttc ttttttcttt ttgataattc ctgaattgct
     3241 agtaaataaa ttagtatagt agtcgtttta atttgtacca tacctaccct ccaaattatt
     3301 tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat catcgcatag taactatcac
     3361 catcttgtcc tttttctgca attgaatcag taaaaatcgt tgaatagata ggaatattgg
     3421 tatcttttga tactgttttc attggtctat catccacact gctttctacg aataaggagg
     3481 gaacttttgt tgtgcgtaat ttttcaacta agtgttttat ttgatctggt gttccttctt
     3541 cttcagtgtt gatttcccaa atgtaagcag aaggcacatt atacgctttc gagaaatatt
     3601 taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa gcaaatttag
     3661 atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga ttttctttat
     3721 agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt ttagcgtaaa
     3781 taataccgtt ttctaaattt aaccaagcat gaggatcttc cttccctttc tcactctgac
     3841 cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg ttttcctctt
     3901 tgttcgcatt tttaactaat tttgtaaacc aagcatttcc tccagtttcc aagttaacac
     3961 cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc aaaggttcat
     4021 attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta tcttttgcta
     4081 tattttcggt aatatctgct aaaatcgaat tagtaactac tacttttaat ttttcgttcg
     4141 aagccacttt atctttgtct ttactattta ttgaacatgc cccgaatata aaaagagcaa
     4201 tcaaaatccc cagacttccc agtactgctt tccattttct catcataacg atctctcctt
     4261 ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata agaatgccgc
     4321 tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaataa acaatccaag
     4381 caatgatgaa cttgctccga ctgttgcaga aataaatatc atagttttca agctatttgc
     4441 atataaatat gctgtagctg ctggtgtaat taacattgca acaattaata tagttcccac
     4501 actttgcata gctgttactg ctactaaagt taatagaacc atcaatagat aatgataaaa
     4561 actgacattc atccctattg cttttgccat taatggatca aaagaagtga ttaataattc
     4621 tttaaaaaat agaactacta taaccaacac taaaaccgaa acgccaattg taatccactt
     4681 atcaatatct tgtacagcta aaatatttcc aaataaaata tgaaataaat ccgttgagct
     4741 atttgcaact cctataagga taactcctag tgctaaaaaa gaactgaaag taataccaat
     4801 tgctgtatct ccttttatta cactattgct ttttataaaa gtaataatga tagaagctaa
     4861 tagaccaaat acaatcgcac caataaaaaa attaatacct aaaatataag aaattgctac
     4921 tcctggcaaa acagcatgag aaatagcatc acccataaga gacattcctc ttaaaataat
     4981 aaaacaacct actgttccag aaacaatccc tataactaca gatgtaatta gagcattctg
     5041 taaaaagtga aagtcataca gaccattaat aaaattttga agcatattat tccccctctc
     5101 caataaatat agtatctcca taggcttttt ttaaattttc ttcattaaat acagattcta
     5161 cacttccgtg agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta
     5221 ccttgcttaa atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattttttta
     5281 acgtatccat aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa
     5341 atatgaaatc cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc
     5401 cagataattc tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct
     5461 ttgaaactct ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg
     5521 ttcccataga tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt
     5581 gctctacata tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct
     5641 ctccctgatg agggataata tttaacattg ctttaagcaa tgtggatttt ccagcaccat
     5701 ttggaccaat aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg
     5761 ctaaaaaatc gttataacaa acattaagct ttttcacttc taacataact ttctccttct
     5821 aaatgataat gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa
     5881 attttatagt ttagattttt ttctgttgct gaagattaga atgacaaaac tatttgaaac
     5941 tacgcgtata gtaagtagct ttcaaaatat ataaaaaagg agcgttctca aatgagaaca
     6001 tgctcctaaa actactttat tggcgtatgg aaaaattata cttgaagtgg atagcgggtt
     6061 gaataagttg ataagcatca aaacatttat aaaaattatt caagactatg tctgttccat
     6121 atacaaatat gaaaatcagt aaactccatt taactttttt cgtagtgtgt ctttttatac
     6181 atgtttatcg ttattaatag tgaataggaa aaaaactatt tttgatcatt gacaattaaa
     6241 tacaattttt gaataacgat ccctgctatt cgagctagaa actcaagtac gccatgacct
     6301 gattagcgac aaatacttag agatttcttt ttgttctggg aaaacaaaat gccatgacaa
     6361 tagcagcaaa taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc
     6421 tatgtgctta cccaaagcaa ttcaaaaatt tcataactac tattttagga gatgatttta
     6481 tagaatccag agtctacctg aataaaaaac aaacctgtgc atttctagga atcgataata
     6541 atactctaga taagtggatt cgtatatata atttcccagt aataagagtt ggtggtgtag
     6601 ttcgctttga cgtagaagat attaaagact ttatgaatac atttaaaata acttgaaatt
     6661 ttcttctcgc cgcatatact aaaattagtg aaatatactg ccttactttt tttaaggagt
     6721 gaggattttg cctacaaagc tttcaaatgg gaaatataaa actaatctga gatatccaaa
     6781 aaggtttaaa gaaataacag gaattgcatc agaaaagtat caaaaaacat ttccaaatag
     6841 gcaattggcg attaaagccg aaaatgatat gaagaagaaa atagaaaaag ttttgagaga
     6901 ggagaatgca aactcattag aattaaaagg aaaaataacg tttaaaaaat tttacgaatc
     6961 taaatggctt cctagatatg aattaggtca aacaattaga tcaaacaggc ctcctagtga
     7021 tataaccatt agtaatacaa aagatatatt tcggttgcat attttaccta tgtttggtga
     7081 atacgcaatg aattatctaa atatcaatac tgagataata tcagatgaat taaccaaaaa
     7141 atcgaaagaa tatgcaaata taaaaatcat aaaaggatat gttcgttcca tgttcgatat
     7201 tgcagaaata ctaaattata tcgaatttaa tcgaactact aaaattattc aaagtatcac
     7261 tgcacctaaa aaaaatgctt tagaagaaaa aagaatacaa gaaggtaagc aagcactatc
     7321 atcaaaagaa cttactaatt ggatagaagc agtaaatgat gattttaata atcatttact
     7381 tacttttcat gattacacac tttttatgat taccctgtat cttggtgata gaaaaagtga
     7441 gacttacgct ttgcaatgga aatatattga ttttgaaaaa caaacggtcc gtcttaaaca
     7501 tactttagat aaatatcaaa gaaaaaagtt tacaaaaggt cgaaaagata ccgtcattca
     7561 agtaccagaa gttgtaatga cattgctaag tgaatggaaa tcggtacaag ctgatcaatt
     7621 gttgaaatta aaaattaagc aaacgttaga tcagtatctc tttacttata ctaaaccatc
     7681 tggagaagta aattgtcctg ttcatgccga ttacctaaac tatcgaatca attcaattaa
     7741 aagaaggcat cctgacttag ttcatttatc tccacacaaa ctccgtcata cttatgcaac
     7801 tatagctaga caaggtggag cagatatgaa tcaaatttca aatgctttaa cacactcaga
     7861 tatttctaca acaaagatat atgtgaacac accagatata gttgataagg ctgtttttga
     7921 agcttttcag agaggattga ataagtgtga ttgatatgtg ttcttaaatg tgttcttaaa
     7981 aacaaaagaa gcactttcca aataattgaa aagtgccaga aacgttgata ta
//
LOCUS       DAJSBC010000102         7846 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSBC010000102 DAJSBC010000000
VERSION     DAJSBC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.104"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(7..2025)
                     /locus_tag="ONT65_002515"
     CDS             complement(7..2025)
                     /locus_tag="ONT65_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR2330104.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2038..3036)
                     /locus_tag="ONT65_002516"
     CDS             complement(2038..3036)
                     /locus_tag="ONT65_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330105.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            3234..4475
                     /gene="sstT"
                     /locus_tag="ONT65_002517"
     CDS             3234..4475
                     /gene="sstT"
                     /locus_tag="ONT65_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR2330106.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4622..5257
                     /locus_tag="ONT65_002518"
     CDS             4622..5257
                     /locus_tag="ONT65_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2330107.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            5448..6188
                     /gene="truA"
                     /locus_tag="ONT65_002519"
     CDS             5448..6188
                     /gene="truA"
                     /locus_tag="ONT65_002519"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2330108.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6333..7712)
                     /locus_tag="ONT65_002520"
     CDS             complement(6333..7712)
                     /locus_tag="ONT65_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330109.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT         2581 a         1226 c         1471 g         2568 t
ORIGIN      
        1 tatttgttag tcttttattt tttgtgtcgc ccatgttgca ctgtactggt tacctgcaac
       61 aagatcatat tgtatcagac gataatcttc tagaatttct ttttgtttct cagttaaatt
      121 tttttcttgg ataatttgac tatcctctgt aacaaagacc tgttttccat taaaacgatt
      181 agacaagctt tctgtaggat caattgttaa tgcaggtaga tgattagata cttctgtcaa
      241 caacgcatag taaggagtta ctttgatatt cgcatgctca agagccatag caccaaagtt
      301 attaggagca acaacttctt tatttaacgt agctgcttgt tcacgactat acttgttgtt
      361 ataaatgaaa taatctgtct ggtgctgttc taatccatat tttttcgaat cattcccact
      421 atagattgcg ggtaaatggt caccataaaa aacaaaggta atcggtttat cgattttgtc
      481 gatttcttgg atgaattttt ttactgctgt atcggtatag tgaaggcctt gcatataagt
      541 ttgtaattct tttcttctat taggaatgac agcagttcct tcagcagtgt agttcaattc
      601 actatagtaa tcatcataag gcatatggtt ttgcatagtg gataattgga tgaattgtga
      661 cgtttccgta ttgctttgta aagctttcaa ggtttctttg tatgctgaat catccgatat
      721 gcgtgggctt tgttctattt tatccgtata agttaattta tccggactgt ctaaataata
      781 gaacctgtca aaaccaaatt tatcaaacac ttgttttctg cgataaagag cagctgtaaa
      841 aggatggatg gctatttttt catcgaatag atcggtaata tttggactta ttttctgacg
      901 ctccactagc tgggtataag gtgtaactaa cgaagcagac aggttactga tactcaagcc
      961 tgtcagtcct tcccattcca tgttagctgt tccgccacca taaccgacgg ataacatcaa
     1021 tcctcctgtt ccttctgtta taatgctttt gatgtaagga atagggtctg taggtactgt
     1081 cagattaggg acacgtgcag gatcactgaa gctttcactt aaattgagaa cgatcgtctg
     1141 attgtttaac cagtcacttc ttgtttggtt gatttcatct gcctttgtgt tgtacttttt
     1201 cattattcca ttaatttttt ctttcgaata gccagcaggt tcttccatga ttttaatgtc
     1261 gatattgttt aggaattgaa tgactggtcc gttatctttt acggcgcctt gctggttaaa
     1321 gaaagtcttg tttactttaa ataaattaaa cagcaagaaa gaaggcgaat ttttgtgatt
     1381 aatgaaaaag acacccgaaa aacatcctaa taatacagcg atactgataa atcgacgttt
     1441 ccaatgaatc ttcaaggaat actgtttttg tagtctacgt tgtacaataa tactagtaat
     1501 caataatagg agaaggatca atccgcccac gattaataag actgggctaa ccatcgataa
     1561 tagttctgac aatccggtca gcatttttaa atctgaagga aagacaggct cttcccgcat
     1621 ttccattttg attactgtcg aaatagtgag cagtaagtta atgatcaagg tagcagtcaa
     1681 ggcgtaccaa aaacgattgg aaatcaacaa tagtaagaag aaaaatccca taataatgat
     1741 gattgtcagg atcatgggtg cttgtcgctg agtaaatatt tttaaggcaa cctggatagt
     1801 ttctttccaa ccttcttttg gttctaaaac gaaaatctgc acgaaggtaa agcagtaaaa
     1861 aaatgctgca actaaaaaat aacttttatg aacaaaaagc caatgaattc tttttttcca
     1921 acgctgagtt aattgttcaa tcgaatcaaa agcgaagtat tcgatgaaac gatgaatgaa
     1981 acggattttt tgtatataaa ataaaaagat aactaggagg gacactgcta ataaatatca
     2041 aataaaaaac tgcccgatat ttatcagcca tgccttccct gagtttggaa attcacgttt
     2101 cataaatgta gatacgcgat aagaaactaa agggaaatta catataactt gagtgacaat
     2161 caaaaataca gcaagaaaat ttcctcttcc ttttatacct aatttatcag atagcatttc
     2221 taaaaataaa aacgaagaga aagtataata cattgcgaag agagaatatg gtttagaaaa
     2281 cctcagcgct gtatctgtat ttcctctgat gatgagtgaa atttgtgtca tcaaaataat
     2341 caaaccgaat aaaacaatca agctgataaa tacttgagga agcttatagc ggaccttttc
     2401 tttccagttc cattctttca aaccataacc cattaaaaat aaataaaaaa tccataaaat
     2461 actctgtcca ttctgtaatg caaacgggtc tttattaaat agagtagagc agccaactaa
     2521 tccaattgtt aaaaataata gtagatattt aattgttgtt ttcgataatt tatttaaaaa
     2581 tccaactgta taaggaatac tacaaaaaag caaagtacaa gaaacagcat aagtaaagta
     2641 attctgactg ataggtgtta ggataagcca ataatcacga atattcaagc tgccagcttg
     2701 tacgtaaaaa aagatattgc aaactagtgt ggctattagg aatataaagg caaaacgact
     2761 taaataatgt aaaactttta aacgggcgat tttttgatat tggtaaccca tgtaaagtgg
     2821 gatcaaattt gcaccaataa caacggaagt ttgtaaaaat gtatagatgt agaatcgacc
     2881 agggtttgtt gcatatgtag gaatagtgac tgctgaaatc aatatacgct gtaaaaccat
     2941 cattatacaa gcgacaaaga ttgtcaggaa gccataactc tccttcttta tttttttcaa
     3001 gaataaacac ctcacaaaaa aattggacaa aaacataaga aaacgtttcc tgataagttg
     3061 cccattaata acatcctaac ttagaatatc tcaaaaaaca acgtgtagac agaaaaagta
     3121 caaaattagc aagaaatggt tgtttgtatt gttagaattt ttagaaaatt atacctttag
     3181 tttctttgca taaaatgata caatgattgt taaaattgtg aaagggtggt catttgttag
     3241 agaagttttt ggatatgtcg ttagttcaac gtattgttgt tggtatttta gtcggaatat
     3301 tgtttggtat ctttttcccc acatggacat ttatttcaat attgggaacg ttgtttgtca
     3361 gttgcttgaa ggcaatcgca cctattctgg tttttttctt gacgatggcg tcaattgcca
     3421 agcataaaat tgggaataag acattcgtga agcctatttt gatcttatat ttagtgggaa
     3481 cattgctttc tgcacttgta gcagttattg taagttcaat ttttactgtt cctattactt
     3541 tacaagaaac agtatctgaa aaagcaccgc aaagtttgga aagtgtttta tctactatgt
     3601 tgacgaatgt cacacaaaat cctgttcaat ctcttattga tgcgaattac cttggcgtct
     3661 tattctgggc tgtattgttg ggactagctt ttcgagcacg ttcagaatcg acaaaagagt
     3721 tggtggatca gatatctata gcgttgtcac aagtcgtaca agtgattatt gcatttgctc
     3781 cgatagggat tttaggattg gtttatcaat caattgctac gactggaatc gctggattgg
     3841 cagagtatct gcagctttta cttgttttga tcggtacgat gctgtttgtc gcattagttg
     3901 tgtacccatt cctcacgttt ttgtttatta aagaaaatcc ctatccattg attttctttt
     3961 gtttgaaaga aagcgcactt cccgctttct ttacccgaag ttctgccgca aatatcccga
     4021 taaacatgat gttggctgaa agattgaaat tgacaaaaga gtcttattcc atatcgatcc
     4081 cgttaggtgc tacgatcaat atgggcggtg ccgcgattac catttccttg atgacgttga
     4141 ctgctgtaca ttctttaggg attgaagcac cttttgtttt gaaagtcctt ttatgtattt
     4201 tgtctgcttt agctgcttgt ggagcttcag gtgttgcggg cggttcactg ttattgattc
     4261 cattagcatg tagtttgttt ggtatctcta atgatgtcgc gatgcaaatc gtcggtattg
     4321 gatttattat cggtgttatc caggattcaa tggaaactgc cttaaattcg tcaagtgatc
     4381 tgttgtttac tgcaatagga gagctaggcg cgcgtaaaag aagtggggaa aaaatcaaat
     4441 tgaaagattg tttagatgga ttaagtgaac gttgaacgtt agatatttag tatttttaaa
     4501 atgattcgag tttttaatga gttatataga tgttcgtgta aatagaggtt tctgttaaat
     4561 caagataaag tcgtttataa taacaattaa aagcataggt aattgttatt ggaggcgtat
     4621 catggatttt ttacgtaaat ttggtttttt ctttttatta ttaggcgtgc tgagtgattt
     4681 tttgactccg tacgtattag gattgtatta tcctgaatta gatcaaatga agagcgtgat
     4741 cagtgtattt ggagaggttt ccagtcctgt aagaaaagcg tttcttattt ggtctgtggt
     4801 ttcagggatc tttttcatcc tgtcattgcc tgcattgtat gctgcatttg ttagtacatc
     4861 tcgtgtgtta gctaccctag ttacagcagc catcgcgttc tatggtatag gtgattgtat
     4921 ctttacaggt ttgttcagta tcaataccca tgaatcttcg tggaatgtct cgacatgggt
     4981 ccataatatc ggatcaggtt taggttacag tggtttttta ttgtttccag ttcttctttt
     5041 cttgatctat cgaaaaaatg ggcaaatgga cgtaagccac ttataccttg gactgtctgt
     5101 gatcagtttg ctttttgcgg cgatttatgg actggcaaga attccttcta taaattcttg
     5161 gccattactg aataaaatag gtttttgcca aagaatcagt tttttcttta attatgtgcc
     5221 cattgccagg ttgagtatct tgcatctcaa acagtaaaac ttgagccgtc aaattgaaca
     5281 gtcagcggat acttcatccg gtggctgttt tttgtttttc tgaaatatat atttccacag
     5341 ttttcttgta tagcttctgt cttagagaag caaggagcta tattttgtgc tacaatctta
     5401 aaaaggtaga gagaagacca aatggaagac agaggagcca ctaaaatatg agaaacatca
     5461 agttgacaat cgaatatgac ggaaaaagat attcaggatg gcaaagactt ggagatgacg
     5521 ataaaaccat tcaaggaaaa atcgaaaaag tattgcatca gatgacaaat gaagagatag
     5581 aaattattgg atcaggaaga acagatgctg gcacccatgc gcgaggacaa gtagcaaatt
     5641 ttaaaacaaa cacagaaatt ggactggcag aaatgatcga ctttatgaat cgttaccttc
     5701 cgcgggatat tgtcatcaaa agaatcgaag aaatgcctga gcgcttccat tcaagatata
     5761 atgcggtggg caaaaaatac agctattatg tttggaacaa cgtgattcct tcagcatttg
     5821 agcgaaacca cagcttttat ttccctcaag agttggatat ggacaagctg aatgcagctt
     5881 gcgaaaaatt agtgggtaca catgatttca ttggtttttc tgcattgaaa aaatcaaaaa
     5941 aatccacagt gcgtacgatt gatgaattat ctatcgaacg aaagggagaa atgcttcatt
     6001 ttacttttgt cggagacgga ttcttatata aaatggtgcg gattatcatg gggacattac
     6061 tagaaatcgg cacaggtgcc atgccgatag aaacaatcga tgaaatattt gaaagcaaag
     6121 tgcggcaaca tgcaggagaa accgtccctg cacaaggtct gtttttggat gaagtttatt
     6181 atcgataaaa atactttgat aatggatgaa acgcatgttt cacagtataa atatcgatgt
     6241 taaataatgt atgaagcaaa ttaaagaaat ataaaaaaag actttttagc ctgtttatgt
     6301 gtacagctaa aaagtctttc ttaaataaaa atctagtgag aatgttgtac ttctcttctc
     6361 ggttgttttt gtttagaaaa agtaaatcga taaagtaaaa ataaaatcaa agtaacgata
     6421 gataaatatc ttgaagcaat tgctagtttt gtgtactgat aagtaataac gactttattt
     6481 atacctggag gaatagttaa ttcagttgtg ccaaacttag ataattttgt ttgtactagt
     6541 ttgccattaa tagaagccac ttgcccttta taacgataaa taggtgtcgt caagacagta
     6601 tttttattat tcgaattatc taattcaatt gtaagtttgt tactcataaa atgagttgtt
     6661 ggatttattt cctggttacc tagtaaatat tgatcattat taatcatatc aggatgatag
     6721 attgactctt tgtttgcata atcagtatga ttgtaatttt cagctatagc aacagcgtct
     6781 tcaggtgcta taagtgttaa tgaatctttg ttagcatgga tagaattgct tactcctgag
     6841 agatgcaata ttagtgcaag aaacgtaaag accattattt tccatgaatt atttttagta
     6901 gaaaaaatat aatagatcac tatactcatc gtataggcta taaataaaga agaaaaacca
     6961 tttaaccgcc agacaaattg tagactctta actggtgtat ggttaaaaag ttgccaagga
     7021 aataaattgg ttgaacaaaa aagaacaaat acaccaagaa agaaaataaa taaatcatca
     7081 gcagttaatt tttttataaa aataaaagca ccaatcaatc cgagaaataa gaataatcca
     7141 atagtatagt tgttatagtt attatttaaa atatgtgtca gcatatcact aggagccata
     7201 ccacttaatt cttttcccag tgggactttc aattcttgtg ctctagattg ttgaataaaa
     7261 ggaattaaga atcccgcact taaaaaaaag gtgaggacgg tagcttttaa aagacttaag
     7321 agtctagcga ttttttggtc ccaaaaataa aaagaaagaa acaacgttcc tccaataaat
     7381 acagaaacca tagcgacaga aagtaaatga gtatacacta ctagcgtcat gccaatagac
     7441 aaccagtacc atttttggta atctctaata taaatttcgt aacaccccat tagtatcaaa
     7501 ggcaaaaatg ttaaagcgac agcttctccc aatgaagcac gatggaatat gtcaatggca
     7561 cgataggcgg aaaatgtata aataatcgaa aataacagcg aaatataacg attgtttttt
     7621 atttgtttca tagaaaaata agaaacgacc attgttaaaa aagtgataaa aaaatagtag
     7681 atattataac ttaaaacaag atttcccatc attttataaa ataaaaaagc tggataaaga
     7741 gtaagccatg gataaaaaat attcatcatt gttccatgat gatcgaagtt attgaagttt
     7801 actggagaac tccatacatt atctagtcct atgattcgag ataaat
//
LOCUS       DAJSBC010000103         7834 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSBC010000103 DAJSBC010000000
VERSION     DAJSBC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.105"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..572
                     /locus_tag="ONT65_002521"
     CDS             <1..572
                     /locus_tag="ONT65_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330110.1"
                     /translation="LYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQP
                     LLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLE
                     NDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFC
                     EHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            559..1980
                     /locus_tag="ONT65_002522"
     CDS             559..1980
                     /locus_tag="ONT65_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2330111.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1993..3000
                     /locus_tag="ONT65_002523"
     CDS             1993..3000
                     /locus_tag="ONT65_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2330112.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            3012..4016
                     /gene="neuB"
                     /locus_tag="ONT65_002524"
     CDS             3012..4016
                     /gene="neuB"
                     /locus_tag="ONT65_002524"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR2330113.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            4013..5161
                     /gene="neuC"
                     /locus_tag="ONT65_002525"
     CDS             4013..5161
                     /gene="neuC"
                     /locus_tag="ONT65_002525"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR2330114.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5134..5811
                     /locus_tag="ONT65_002526"
     CDS             5134..5811
                     /locus_tag="ONT65_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2330115.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5801..6943
                     /locus_tag="ONT65_002527"
     CDS             5801..6943
                     /locus_tag="ONT65_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR2330116.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            6956..>7834
                     /locus_tag="ONT65_002528"
     CDS             6956..>7834
                     /locus_tag="ONT65_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2330117.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIK"
BASE COUNT         2838 a          996 c         1436 g         2564 t
ORIGIN      
        1 tactttatga tgtaaaaaat ataaaaaaaa ttcccaaaat agatgaaaac agtaaattga
       61 tgaaaaacat acaatcaatc tttgggatag aagaagtttt tgtcggagat aaatgtatct
      121 tttttgatca acctttatta tctgatggtt atggcataga tgaaacagat attttcgcta
      181 aagtaaatag tatattttct ggaaaaaaag aaactttaat taagcttcat cctagagcaa
      241 ggacaaatcg ttatggtgaa aaaaacatat tgaattccag tttaccgttt gaagtcttgc
      301 tacttgaaaa tgacttctct tcaacacttt tgattagtcc tatatctaca atttcttttt
      361 caccatctat gatgtttgaa aagacgatca actctattat gttggctaaa ttaattttaa
      421 atgaatatac agcattatct gaagataaaa aagaaacatt gacaaatatt gttcttttct
      481 gtgaacatta taaccgtcta tcagaagaaa aaattttact cccagaaaac tgggcagaat
      541 taagaaagtt ggtagagtat gggattattt aacacacttt ccaagtcaaa attagttaag
      601 tccagtttct ggtatactat agggaatttt tttattaagg gaatttcatt cataactatt
      661 cctatttttg taaatattac tactcaagca gaatatggtt tgattaataa tttcacttca
      721 tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa attcggcaat taataatgca
      781 agttttgact ataaagatga cataaatgaa tttatgtcat cggtactttc tctagctaca
      841 ctttcgttaa ttgtgttctt attaataggg aacatatatt ttttgttttc aaatgctttt
      901 tttgatatga accaacttgt atttaatttg atgttattac aaagttttgc tagttttgta
      961 attagttata tccagtctta ttttacgatt aactttaacc attttaaata tttgatttta
     1021 tcttttatat ctactttttt gaatattgca atctctgtag ctttgatgca gacattatat
     1081 tttgatgata aatatgttgg cagagtagtc ggcggaacac tgtcatttgt gttgataggt
     1141 ttagtaattt atttaagaat aatgataaag ggtgctcatt tttttgataa aaaacagtgg
     1201 agatacgctt taaaaatctc tttgccgcta atcccacatt ccttagcaaa tatagtttta
     1261 gcacaattcg atagaattat gattaattct tatttaggtc ctgctgcggg gggaatatat
     1321 tcctacatta gtaacatagg tattattcta agcgtaatat ggatgtctac taataacgcc
     1381 tgggttccat ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt gaagaaagtt
     1441 agtaactatt atttagtagt ctttactttg attacgatga ttgtaatggt agtttcgatt
     1501 gatttagcaa aatttatggc tccgaaagac tattattctg ggttatcttt agtggttccg
     1561 ataaccttag gctatttctt tcagttttta tatagtttac cagttaatgc tgaattttat
     1621 gagaaaaaaa catcgtttat tgcgattggt acagtagcaa gtgctatagt taatatacta
     1681 ttaaacatga ttttcatacc taggttagga ataattgctg ctggatacac aaccgtattt
     1741 acgtattttt tactttttat ttttcattat aatatcgcta aaaaaattac gaataaacaa
     1801 ttatttgaca caaaaaaaat atgtatcata ataatttttg tatcaatttt ttctttgata
     1861 cttcatattt taatcaatca aatgttgatt cgttacgttt tgacatcatt attgataatt
     1921 agtttagtta tatatttttt aaagattaag aatcgaatta tagatgacta taattcatag
     1981 gaggagatat aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt
     2041 ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt
     2101 agaggtgaag aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga
     2161 tttttaattg gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat
     2221 attatttttc atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa
     2281 gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat
     2341 gtaaaacatt ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt
     2401 gcgacaaagt tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca
     2461 gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt
     2521 cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg
     2581 acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat
     2641 tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct
     2701 caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa
     2761 gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt
     2821 gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac
     2881 gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa
     2941 ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa
     3001 ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg
     3061 gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg
     3121 ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat
     3181 atcagaagga taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat
     3241 tatctcaatc tgattttatt agaataaaag attattgtat acaaaataaa attgattttt
     3301 tgtctactcc ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat
     3361 ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg
     3421 ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag
     3481 attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt
     3541 ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg
     3601 ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta
     3661 aggtaattga gaaacatttt accttggata aggaactacc tggtccagat cataaagctt
     3721 ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt
     3781 taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa
     3841 aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta
     3901 caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa
     3961 aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa
     4021 aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa
     4081 tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa
     4141 atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca
     4201 aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa
     4261 tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac taggggatag
     4321 aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat
     4381 tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat
     4441 agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga
     4501 agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag
     4561 attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata
     4621 cgttttgcaa gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt
     4681 agctttgatc aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag
     4741 tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat
     4801 ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat
     4861 gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa
     4921 tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa
     4981 tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt
     5041 gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt
     5101 gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta
     5161 aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt
     5221 gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa
     5281 atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa
     5341 aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata
     5401 aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg
     5461 gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca
     5521 gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt
     5581 agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta
     5641 ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct
     5701 tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct
     5761 tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt
     5821 atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa
     5881 ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac
     5941 tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt
     6001 aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc
     6061 tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa
     6121 tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa
     6181 ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg
     6241 aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt
     6301 aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt
     6361 taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa
     6421 atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca
     6481 acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg
     6541 acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa
     6601 ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc
     6661 aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa
     6721 cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga aaccactaca
     6781 tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc ttagtgtgtc
     6841 agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga cagatagtga
     6901 acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat attttatgaa
     6961 cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca ttcaagaaca
     7021 ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa aggaagttga
     7081 aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt tgtttgaaat
     7141 aaattcagtt tttgtttgta catcccctat aacccatgaa tttattataa aagaggcttt
     7201 aaatttagat tgtaatatct ttacggaaat caatttatta aatgaatatt atgatgaaat
     7261 aatcgaagta gctaaaaaga aagatagatt actttatcta tcttctacat ttttgaagag
     7321 aaaagaaatt caatatatta agtctaaagt gacgcataat aaaaaaattt cttatcaata
     7381 ccatgtagga cagtatttac ccgactggca tccttgggaa gattatcgca atttttttgt
     7441 ttcaaacaaa aaaactaatg ggtgtagaga aatattcgct attgaattac cttggattat
     7501 agatacattt gggaaagtta aatcgtttac tgtaatgacc cagaaattat ccaatcttga
     7561 tattgattat tcggacagtt tcactcttat tattgagcat gaaactggta tagttggaac
     7621 tttaagtgta gatgttgttt caagagtagc taaaagaaat ttaactataa ttggtgaaaa
     7681 gattttaata gaatgggaag gtaacccgga ttctctattt tcatggaatg aagaaacttc
     7741 tagtatgaca gatattcaat tgtacgatga ggttatgcat gataataatt attcacatac
     7801 tattattgaa gatgcatatt tagaggagat taaa
//
LOCUS       DAJSBC010000104         7811 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSBC010000104 DAJSBC010000000
VERSION     DAJSBC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.106"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            342..815
                     /locus_tag="ONT65_002529"
     CDS             342..815
                     /locus_tag="ONT65_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR2330118.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            812..2944
                     /gene="feoB"
                     /locus_tag="ONT65_002530"
     CDS             812..2944
                     /gene="feoB"
                     /locus_tag="ONT65_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR2330119.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2946..3080
                     /locus_tag="ONT65_002531"
     CDS             2946..3080
                     /locus_tag="ONT65_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR2330120.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3174..3818
                     /locus_tag="ONT65_002532"
     CDS             3174..3818
                     /locus_tag="ONT65_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2330121.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4005..5198
                     /locus_tag="ONT65_002533"
     CDS             4005..5198
                     /locus_tag="ONT65_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2330122.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5191..6918
                     /locus_tag="ONT65_002534"
     CDS             5191..6918
                     /locus_tag="ONT65_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR2330123.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7312..7509
                     /locus_tag="ONT65_002535"
     CDS             7312..7509
                     /locus_tag="ONT65_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR2330124.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7511..>7811
                     /locus_tag="ONT65_002536"
     CDS             7511..>7811
                     /locus_tag="ONT65_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2330125.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDK"
BASE COUNT         2581 a         1309 c         1708 g         2213 t
ORIGIN      
        1 taaaaaagtc aaacaacaaa cactacatat tgtgttgtgg aaactgtgga atactaagtt
       61 ttgtgttttt gaaaaaagag ctgaaaacaa gtatcgtgtg ggaaaatagc aaatttaata
      121 gagaaaaaat tgtggaaaac tatgaagaaa tatttttttc tcatttttat ctatcaatac
      181 taaagattcg ctcataatat cacaagtata attgacattc tcaattaccg agagtattct
      241 atgtgagaat gattctcatt tatttgcgta acacgtaaga aaattagaac atataatgaa
      301 tgattttaat acatgattta tttcacaaga gaggaatcaa tatgcagacc ttagcgcagg
      361 caaagcagaa tttcgtttat caagtcgaac gaatcgaaac agaagaaaaa acaaaacgcc
      421 atttgcaaaa tatcgggatc gttgccggaa gtaaaatcgt cgtcgtcaat ttatcaggca
      481 acaatgccat tttactggta aaaagtcatc gagtggcagt aagtcgggaa atactaggtc
      541 aaatctttgt caataaaaag actgcttcct ctgaaagctg gacttctctt gatcaattaa
      601 aagcaggaga gactgggatc gtcgtagcaa tccacggaca aggcgcagtg aagcgacggt
      661 taatggatat gggattgacg cgaaatgtaa aaatagttat ccgaaaacta gcccctctgg
      721 gagatccaat cgaactgaca gtgcgagggt atgaactgac gttacgaaag tcagaggccg
      781 aactaatctt gatacaaagg gaggaaaaag aatgaatatc gcgttagtcg gaaatccaaa
      841 cagcgggaaa actagcttgt tcaatctctt gactggttcg aatcaatatg tagggaattg
      901 gccgggcgta acagtagaaa aaaaagaagg atcgttgaaa aaaaatccga acattcatat
      961 tcaggaccta cccggcatct attctttgtc tccttataca ccagaagaag tgattgcaag
     1021 agattatttg gttcaggaac agcctgaact gatcatcaac attattgatg ctaccaattt
     1081 agaacgaaat ctttatttaa ctacgcagtt attggattta gacattccta tgatcattgg
     1141 tctgaatatg atggatgtca tcaaaaaaga aggaaaaaaa atcaatctag aaaagctttc
     1201 ctatggtctt ggtgtaccag ttttaccaat cagcgtattg aaaaagcaag gaatcgaaaa
     1261 actaatcgaa aagattcagg agtctgcaaa aaagcggcct tctattcctg ttccctgtac
     1321 atatgatcca cgtttagaag cagctttaca tgaaattgaa gatgtactag gagaacaaag
     1381 cggcaagaaa cgctggtatg caatgaaata ttttgaacga gatcagaagg taagagagga
     1441 ttacgagata gcagtttctc agcaaaaaga gatcgagcaa ctgatccagt taacagagaa
     1501 actattggac gacgatagtg agacgattct ggtcaatgaa cgttatgaat ttattaccca
     1561 actatgtaca ctctgtgttg tgtcaaatga ttctttccaa ttatcgatga gtgacaaaat
     1621 cgatcagatt gcaacgaatc gttggttagc cttgccgatc ttcgcatttg tgatgtggct
     1681 gatctattat ctggctattc aaacagtagg aacaatgggg acagattgga tcaatgacac
     1741 attgtttggt acatggttac cagaacatgt tggcagattg ttagctgaat ggcaagtagc
     1801 gggatggatg caggagctga tcttgaacgg catcatcgca ggcgtgggag cagtgttagg
     1861 atttctaccg caattgatta tattgttttt atgcttgagc tttctggaag actgcggata
     1921 tatggcgagg atcgcttttg tcatggatcg acttttcagg aaattcggtc tttcaggaaa
     1981 atccttcatt cctatgctga ttgctacagg atgtggagtg ccaggcgtca tggccagtcg
     2041 cacgattgaa aacgagcaag atcgtcgttt gacgatcatg gtcacaacct tcatgccttg
     2101 ttctgcaaag ttgccgatta ttgccttagt agcaggggcc ttttttcctc atcaatcttg
     2161 ggtagctcct tctgcctatt ttcttggtat gactgcaatt attttctctg gaatcagctt
     2221 gaaaaaaaca cgtttgtttg ctggagatac agctcctttt atcatggaat tgcccgcata
     2281 tcattttcct caatggtcag cggtcctaag acaaacctat gaccgcagca agtcttttgt
     2341 taaaaaagca ggaacgatta tctttgtttc tagtatcgtg atctggttca gctcttctta
     2401 taatttttat gctcaacctg tttctgaaga ccaaagtatc ttagcctttt tcgggcgaat
     2461 cctggcagtt gtttttcagc cgcttggttg gggaaactgg caaggaacag tcgcagcgat
     2521 tacaggattg gtcgctaaag aaaatattat tggcacattt ggtattttgt ttggtcatgc
     2581 ggaagtatcc gaaaatggca gagaaatctg gcaagtgcta caacacacat atactcccgc
     2641 agcagcttat tcattgttag ccttcaattt actttgtgcg ccttgttttg cggcaattgg
     2701 ggctatccat cgggaaatga atgccaagaa atggacttgg atcgccatag gctatcaatg
     2761 cggtttagct tatttagtta gttttgtgat ttatcagttg ggacacttat tggagggtgg
     2821 acaggtagcg ttagggacgt atcttgctat tttactgatg ataggactcg tttatgtttt
     2881 gttccgtcag ccaaaatcca agaaccagtt ttatactata ttgtcattag aaggagagga
     2941 ataggatggc gacattctta ttaagtgtgt taatctttgg aagtgcaggc gcaattgtct
     3001 atactcgttt aaaatccggt aagaattgtg atgactgtca aacagcttgc ccagtaaaaa
     3061 aggaacagat caagcgatga tcagtcgttg caagaggcta ttctcccctt ttttgcttat
     3121 tttgctagag catagtaaga tgagggaaag taaaaaaaga aagaggaaag aaaatgtctg
     3181 gaaagaaaaa aatcgtccgc ccgcgctacc agcagattgc tgttgattta gccgaacgta
     3241 tcgttgaaaa ccgatataaa gtgggtgaga agatccatgc ccgctcaaca cttgccagta
     3301 cgtacaatgc ttctcctgag actacccgca aggctatcaa tgtgctggta gatcttggaa
     3361 tcatggaagt acggcatgga agtggggcat ttgttgcttc gaaggaaaaa gcaaaagatt
     3421 tcctattgca atatcaagat gtacaatcca tccaagatac aaaagcagaa atcatggcaa
     3481 gtgtggagaa gcaacaagat gagctgaatc atttttctca attattggat cagctggtca
     3541 atcaaacgaa gcgaatacat aagatgaatc ctttacttcc gtttgaatta tctttatcag
     3601 aaaaagcaat gcatctggac cgttcaatta gcgagttgaa tatctggcag gcgactggcg
     3661 ccacagttat cgcaatttct cataatgaag aactgcttgt ttcacctggc ccctatgcta
     3721 aatttacggc aggggacatc gttcatttta ttggaaatga atatacacta cagcgtatga
     3781 caaacttttt ttacccggag catcagcttg cagaatgatt gtgtgaaata ttaaaaaaat
     3841 attaacttgg ctaaaccttt tatttacaaa gatttagcca agtttttttc agtttggaca
     3901 agcaaatttc tttcattttt gacaaagtga ctacatgatg ttaacatagt gtggtcactt
     3961 ttttcagaaa gaaaaatgaa tttacataaa aaaggagaat ttgtttggta aaagtagaag
     4021 taaaacattt aacaaagata tttggtaaaa agacacaagc cgctttagat atgatgaatg
     4081 atcaccagcc gaaaacggag attttaaaga aaaccggtgc aacggttggt gtgtatgacg
     4141 tgaattttga cgtaaaagaa ggagaaatct tcgtgatcat ggggctttcc gggagtggga
     4201 aatccacatt gatccgtcta ttgaatcgtc tgattgagcc aacttcgggc agtatctata
     4261 ttgatggaga agatgtatcg aagttaagca aagaagaatt gcgagaagtc cgtcgtcata
     4321 aaatcaatat ggttttccaa aacttcggtc tgtttccaca tcgaacgatc ttggaaaata
     4381 cagaatacgg gttagaagtc cgtggtgtac ctaaagaaga acgacaagaa aaagcagaaa
     4441 aagcattaga aaattctagt ttgctttcat ttaaagacca atatcctagt cagttatctg
     4501 gtgggatgca acaacgggtt ggcctagcca gagctttagc aaatgatcct gagatcttgc
     4561 tgatggacga agcgttctcg gctcttgatc cgctgatccg gcgagaaatg caagacgaat
     4621 tactggacct gcaagccaat gttcaaaaaa cgattatttt cattacccat gatttgaatg
     4681 aagctttacg gattggggac agaatcgcct tgatgaaaga cggcgaaatc atgcagatcg
     4741 gaacaggtga agaaatattg accaatccag caaatgatta tgtacgtgaa tttgtggaag
     4801 aagtcgatcg ttccaaagtg cttacggctc aaaacatcat ggtacctgca ttgacgacga
     4861 atatcgaatc tgatggacca aacgttgcat tgacaaggat gcgtaatgaa gaagtcagca
     4921 tgctgatggc tgttgacaga aagagacacc tgaaagggat catcactgca gatcaagcgt
     4981 tagaagctag gaagcaaaaa cgtccattga tcgacttcct agatgaaaat gtgacggtga
     5041 ttgggaaaga catggtggta agtgatattt tcaatattat ttatgactca ccgactccac
     5101 ttgcagttgt agaggatgga aaattaaaag gtgtcgtcat ccgaggtagt gtgattgaag
     5161 cattagcaga aacaagcgag gtgagtgaac atgaataatc tattgaacta tcaattacca
     5221 gtggcaaact gggtagaaaa tatcacagaa tggtttacga caactttttc tggcctattt
     5281 tcatttttac aaacgattgg acaggcagtg atgtcgggga ttacaaattt actgttagtg
     5341 attccagctc ctttgtttat cttgttattg acggtagcag ctttctttat ttctaagaaa
     5401 cgtccaggac ttacgttgtt cacattaatc ggtctatggt ttatttataa ccaaggtcta
     5461 tggaatgatt tgatgaatac tgtcacactt gttcttttat ctagtgtgat ctcgatcatc
     5521 attggggtac cattagggat cttgatggca aaaagcagta aagcccaaag tattatcaaa
     5581 ccgatccttg actttatgca gacaatgcca ggattcgttt atttgattcc agcagttgca
     5641 tttttcggta tcgggatggt tccaggggta tttgcctcag tcatctttgc tctgccacca
     5701 accgttcgtt ttacgaattt agggattcga caagttccta aagaattagt agaagcttct
     5761 gattcattcg ggagcacaag tcgtcaaaaa ctattcaaac tagaattgcc tttagctaaa
     5821 agtacgatca tggcagggat caatcaaaca acaatgctgt cactttcaat ggtcgttatt
     5881 gcctctatga tcggtgcacc cggcttagga cgcggcgtat tatcagcatt gcaaagagca
     5941 caagtcggga atggatttgt caacggggtt gcattagtta tcttagcaat catcattgac
     6001 cgttttacac agcatttgaa ccagccaaac aataaaaaag ctgcaggagc aacaacacag
     6061 aaaagtaaaa aacgtcaagg actgatcatt ggtgctgtag tagtagtgat cttaggtgcg
     6121 attggtatag ggagtttttc atctgccaaa gaaacaaaac gtatcaactt atcttatgtt
     6181 gaatgggata cagaagttgc ttcaacaaat gtagtcggtg aagtattgaa acaaatgggc
     6241 tatgatgtaa cgatgacgcc attagataat tcaatcatgt ggaaatctgt ttcaaatggc
     6301 gagtcagatg ccatggtttc agcttggcta cctaaaactc atggttcaca atatgcacaa
     6361 tataaagatc aagtcgaaga cctaggtgcc aatttgacag gagcaaaagt tggtttagca
     6421 gtgccagcct acatggatgt aaattcgatc gatgagttaa ccgatcaggc aggtaagaaa
     6481 atcataggaa tcgagccagg agctggtgta gttacggctg ctgaaaacac gatccaaaaa
     6541 tatgacaact tgaaagactg gaaagtagag acatcttctt caggagcaat gaccgttgca
     6601 ctaggtcaag cgatcaaaaa acacgaacca attgtagtta caggatggac acctcattgg
     6661 atgtttgcca aatatgatct gaaatactta gaagatcctg aaaacggaat gggaagcgaa
     6721 gagcagatcc atacgatggt tcgaaaagga ttgaaagaag accagccaga agcttacaaa
     6781 gtattagaca atttccattg gtcagaaaaa gatatggaaa aagttatgtt ggaaatcaac
     6841 aatggaaaag atccacagca agcagcaaaa gactggatca aagagaacca agaattagtc
     6901 gaaagctgga aaaaataaaa agacagacac gaaataaatg gagcttccaa aattggatca
     6961 aatatccaaa tttggaagct ccatgttcgt ctctatcttt tttgtctata aaaaaagaga
     7021 atgcgtagct catcttcgaa acgtccaaag acgccgacaa actattatga atttatgggt
     7081 gcgagtcaaa gagatcgatc gtaggtatga aagactaatg tgattaaagt ttattatgta
     7141 cgaagtgcat tctcaaaaaa tattaaagaa tatacatagt ttatatactt aaaagtgatt
     7201 aaattcttat tcaggaatta ttttgctacg tttcaagaag gaattgtttt ttgaaattta
     7261 atgaatgtta gaataatagt ctaaaaacat taatttaggg gtgattagat tatgaaacat
     7321 ttaaaaattt tgtctattaa agagacacaa cttatctatg ggggtaccac tcatagtgga
     7381 aaatattatg gaaatggagt gtattgcact aaaaataaat gtacggtcga ttgggccaag
     7441 gcaactactt gtattgcagg aatgtctata ggtggttttt taggtggagc aattccaggg
     7501 aagtgctaaa atgaaaaaaa atgctaagca aattgttcat gaattatata atgatatatc
     7561 tataagtaaa gatcctaaat attctgatat tcttgaggtt ttacaaaagg tatatttaaa
     7621 attagaaaaa caaaaatatg aattagatcc cggtccttta ataaatagat tggtgaatta
     7681 tctatatttt actgcttata ctaataaaat aagattcact gaatatcaag aggaattaat
     7741 aagaaatttg agtgaaattg gaagaactgc tggaataaat ggtttatatc gagcagatta
     7801 tggagataaa t
//
LOCUS       DAJSBC010000105         7681 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSBC010000105 DAJSBC010000000
VERSION     DAJSBC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.107"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(82..1230)
                     /gene="nagA"
                     /locus_tag="ONT65_002537"
     CDS             complement(82..1230)
                     /gene="nagA"
                     /locus_tag="ONT65_002537"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR2330126.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1306..2142)
                     /locus_tag="ONT65_002538"
     CDS             complement(1306..2142)
                     /locus_tag="ONT65_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2330127.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2326..3543)
                     /locus_tag="ONT65_002539"
     CDS             complement(2326..3543)
                     /locus_tag="ONT65_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2330128.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3692..3871)
                     /locus_tag="ONT65_002540"
     CDS             complement(3692..3871)
                     /locus_tag="ONT65_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330129.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3926..4009
                     /locus_tag="ONT65_002541"
     tRNA            3926..4009
                     /locus_tag="ONT65_002541"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3960..3962,aa:Leu,seq:gag)
     gene            complement(4249..5619)
                     /locus_tag="ONT65_002542"
     CDS             complement(4249..5619)
                     /locus_tag="ONT65_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330130.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6333..6623)
                     /locus_tag="ONT65_002543"
     CDS             complement(6333..6623)
                     /locus_tag="ONT65_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330131.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6831..7523)
                     /locus_tag="ONT65_002544"
     CDS             complement(6831..7523)
                     /locus_tag="ONT65_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2330132.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2336 a         1525 c         1178 g         2642 t
ORIGIN      
        1 gtaaagtgat ttttactaga acacaaaaaa accttaaaac caaacttaag aagtttagtt
       61 ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta gataagttgc
      121 ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt gagcgatttt
      181 tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca tcatgactgc
      241 ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt tcaatttcaa
      301 tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta ctactactgg
      361 aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac agtctgtaat
      421 taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat ttggatgtac
      481 atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata acgcacccac
      541 catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga caaatacact
      601 tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt gtcctagaga
      661 aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt gacgttccgg
      721 tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat ggaaagtatc
      781 caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt ctgtgaagaa
      841 aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat acattttgcc
      901 gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg ttggtaaaaa
      961 tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt ctgcatcatt
     1021 atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac caggagcaat
     1081 ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt tggtgtattc
     1141 accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg acgctaaaaa
     1201 gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt ttctacctct
     1261 aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca aattttttga
     1321 aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg gttcttcttt
     1381 acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt agctattctt
     1441 ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga tttcattttg
     1501 atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt ttgatcgttc
     1561 atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg cttcagccgt
     1621 ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt ctgtgtcaga
     1681 aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat aataaaggga
     1741 ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat aatagcgttg
     1801 aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta cttgttctaa
     1861 cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt tatcgtttgt
     1921 ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta gccgttgaca
     1981 aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc gacttaatac
     2041 actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt cttttcgaat
     2101 ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc tccccaaaat
     2161 atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc aagaaaagac
     2221 ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag agagcctcag
     2281 tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt tttggcgata
     2341 ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca ttttaggaag
     2401 ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat gtacaagtag
     2461 aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa tattgaatcg
     2521 ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg ctgaaagacc
     2581 aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc ttccgcctgg
     2641 caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa tttgctgaga
     2701 caatacattg gaacacaaac gcatggagga caacatattc agcccttcta tatagccttt
     2761 gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg ctacacgatg
     2821 agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg tagcaatcgg
     2881 aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat aaataatcag
     2941 gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat aaagtgcccc
     3001 agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta tttttgattt
     3061 gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat gaacaggtgt
     3121 gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg gaaccagtac
     3181 ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc tcactccgtt
     3241 gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc aatattgctc
     3301 aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg agtcacgggc
     3361 attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag gtgcgggatt
     3421 tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg cttcttctag
     3481 aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt caaattctct
     3541 catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt ttgttattta
     3601 ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga aaaacgtaag
     3661 tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca ttccatagat
     3721 tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca agcaaaaaac
     3781 gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa aattctccgt
     3841 catatttctc catcttaata agtcctccca tttttgagct tgacaactat gtaaaacctc
     3901 gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca agcatgattg
     3961 agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac tacaactcgt
     4021 gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc attatataca
     4081 tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga ggtgagtata
     4141 tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat taatcgaaaa
     4201 acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt atctgtttat
     4261 caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca taaattcatt
     4321 tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta agatggcgat
     4381 aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg gagaaaatcc
     4441 cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa tagaagtttt
     4501 cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg ccaagactac
     4561 taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta actctgtaaa
     4621 gagattattc aggttactac tactccaaat cattgccctt ttatttccat tgacgtaagt
     4681 aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa acggtaatgc
     4741 agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga aaattaagaa
     4801 aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa atgatgaaat
     4861 acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat
     4921 ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa
     4981 gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa
     5041 actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa
     5101 tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag
     5161 ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt
     5221 ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca
     5281 aaccaataat aaatttttaa catttatttt ctcaaaaaca tacttgcaaa gtaatactac
     5341 aattcctaac ataatcgtag aatttaataa tttccatagc ataaagtgtt tagaaaagaa
     5401 cattaagaat agttctataa taattcttga actccatgtc ttataatgtt ctatcgaagc
     5461 acccaaatag ccaagttcat ctaatctact tgcataaact acgtcatccg ctctcatagt
     5521 cggcatatac atattaaaga gtaaaaagat accccaaatc cctattagta acaagatcag
     5581 ttctttgtta ttcgttattt ttctattatg tttgttcatt tttatactcc ttcttatatt
     5641 tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa agttatcttt
     5701 taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat agtcaacaaa
     5761 aaaattttca ttatatccag aaaaattttt cttagttaag gacctatgga ttgcagtaat
     5821 gcagaattcg tcgaataaac tgccaaagac atacaatcat attgctttta aaattgacga
     5881 acaagatata gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg
     5941 acgttctcgt gtgaaaggcg aggcttcttc aatttacttt tacgactatg ataatcacct
     6001 cttcgagtta cataccggta ctctccagga acgtttaaaa tcctacaatt ccactacctg
     6061 agactagtaa tgactagtct ctttttatgg cctctgatcc ccccattgag cgaatactaa
     6121 tagggcttcg aattgatctg ttcaatcttt aagcaatatc cctcaaatga gtttggaaag
     6181 tttagtgatt catcacaaat acttaaatac atgtagttat atatagattg aggttcttgt
     6241 gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg
     6301 gctaggacat ttttgtctca gccgcttgtt ttctagttta ttctttcatc ttgtcggacc
     6361 ttttttcttg ggtatagact tttctcatat tctaccacaa ttttgggttt ccctaacaca
     6421 tgataggatc tcacaacaaa ttgctttcta ttattataat aacctgtaac gactaacttc
     6481 atcccttcgt ctacttcagc taggaagttc aaactatggg aagcaatcaa acaattgacc
     6541 ccatttaaac tgaaacggat caacggtctg gcactaactt gcaaagtacg gatcttatct
     6601 actgttccat attttttctc cataaccttc acctcaatac cattatacga acatacattc
     6661 cctttgtaaa gaagaaactc tgttttatct ttttgacttt ttgtctatta cctttcttaa
     6721 aatcatattg gcacttcctg cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt
     6781 atagattact gttgcttctt tactaattta ggtttacttg ttaagaaaga ttatttacgc
     6841 aaatgttctt caacaatttg ctgagattta tcagctgtat cttccacttc atcttttgct
     6901 ttttctaact tttgaatata ttgtttcaag cttgagtttt cagcagttgc ttcgtctaat
     6961 ttcttttgtg tatctgttaa ctgagtttct acatttgttt tttcttgtgt cagtgttgcg
     7021 atttgttgat tcaaaccatt tacagtattt tgtaattctg aaagttttgc ttgtgcttct
     7081 tgtagtcttg ctgtccaatc tgcattcaga tgattgattt catcattttt ttggttgatc
     7141 tcatctagtt tgacattgat ttcattttgt ttgctttgta gttgtgcatc cttgctgact
     7201 aattgacttt ctaagttttg tttttctgct gccgattgat ttttttggtt ttctaattca
     7261 tttacttttg tttggtattc ttggacatct gcctttgaat cttgcaatgt ctgttctttt
     7321 tgtgctagtc gagcggtaag atctgtaagc gcttgctctt tatctgtcaa tgtatgatca
     7381 agctgtgtca gattctcatt gattgtatca atatctgtct ctccacccca caaaataatt
     7441 tcatttgcgt accgcgtgcc tgtgtagcca attagacata gaaatgcaac gacaatccca
     7501 ccagtaataa atttcttttt catttgcata gctccttaat ccaatacttt tacatgctaa
     7561 aagtaagtga tctcaaacac aatgtcaagc gtttttaaat tacatgtgta attttaatat
     7621 aaatactatc aaatactcat ttaatcctcc ttttttatct atttaaaaaa tcttttttat
     7681 a
//
LOCUS       DAJSBC010000106         7632 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSBC010000106 DAJSBC010000000
VERSION     DAJSBC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.108"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(62..790)
                     /locus_tag="ONT65_002545"
     CDS             complement(62..790)
                     /locus_tag="ONT65_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2330133.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="ONT65_002546"
     CDS             complement(803..2083)
                     /locus_tag="ONT65_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2330134.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="ONT65_002547"
     CDS             complement(2224..2826)
                     /locus_tag="ONT65_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2330135.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="ONT65_002548"
     CDS             3071..3277
                     /locus_tag="ONT65_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330136.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="ONT65_002549"
     CDS             3589..4308
                     /locus_tag="ONT65_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2330137.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="ONT65_002550"
     CDS             complement(4389..5072)
                     /locus_tag="ONT65_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR2330138.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="ONT65_002551"
     CDS             5290..7476
                     /locus_tag="ONT65_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2330139.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2735 a         1284 c         1438 g         2175 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggtgt
     7621 gacaagaatt tt
//
LOCUS       DAJSBC010000107         7519 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSBC010000107 DAJSBC010000000
VERSION     DAJSBC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.109"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(862..1275)
                     /locus_tag="ONT65_002552"
     CDS             complement(862..1275)
                     /locus_tag="ONT65_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR2330140.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1731..2216)
                     /locus_tag="ONT65_002553"
     CDS             complement(1731..2216)
                     /locus_tag="ONT65_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330141.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2355..2570)
                     /locus_tag="ONT65_002554"
     CDS             complement(2355..2570)
                     /locus_tag="ONT65_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR2330142.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2794..3594
                     /locus_tag="ONT65_002555"
     CDS             2794..3594
                     /locus_tag="ONT65_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2330143.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3613..5481
                     /locus_tag="ONT65_002556"
     CDS             3613..5481
                     /locus_tag="ONT65_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2330144.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5474..5965
                     /locus_tag="ONT65_002557"
     CDS             5474..5965
                     /locus_tag="ONT65_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR2330145.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5953..6507
                     /gene="srlA"
                     /locus_tag="ONT65_002558"
     CDS             5953..6507
                     /gene="srlA"
                     /locus_tag="ONT65_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR2330146.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6525..>7519
                     /locus_tag="ONT65_002559"
     CDS             6525..>7519
                     /locus_tag="ONT65_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR2330147.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2674 a         1204 c         1431 g         2210 t
ORIGIN      
        1 actttcaaaa atatgttttg gaagttggga caaacgagtt aatacatttt aatacattat
       61 tttttattca aaaaaaatag cacaatggca aagatcgtaa gagagatcat aagccccaat
      121 gaatgactaa aaagaaaagc agaatcacat gccatatcat cccatatttc ttcaagaaaa
      181 taaataagcg ggaaaaaggg aaggaatagt agaacatcga tccagcacca tttttttctt
      241 cttttgaaat aacataaaac aataaacatg aacgtgcagg gaagtaaaaa caaagcttta
      301 ctcataacta acaaaatacc agctcctttt atttttttct cccccacatt caaagagtaa
      361 caatggtatg tacttaataa tagaaaaatg gatcaacttg tatgaataac agctgaaaag
      421 atctgcacca aataaaaaac ttccagccaa aactgactgg aagtttattt ttttctgaaa
      481 tattctaaca caaatatgaa aaagatggcc gaagctaata agccgatttc gtagcaaaac
      541 aatgagatac cagagtcgta ataataaaat cctctaatct tatggataaa atagatcatc
      601 ggacaagcag ataagagtaa gtatttgtta ggccttttga ataagtaaaa gaaagcactg
      661 gtcattatga taaatggtat ccaaaacaaa agtttactta agacaatcaa atgatcaact
      721 cccatatttt ttaccaagag agatcataac atctttatcg taaaaacaaa atattttttt
      781 gctcattttg aaaatttaaa catactcgtt cttatccaaa tatataaaaa tcgcccctca
      841 ttcatcgaga ggcgataagt tctaatttaa ctcaattttt tttgtttctt ttggctgata
      901 agctgtttta ggtaatacca atttcagcac accgttttta aaggttccag tgattttttc
      961 ttcatcaaca ttaggaagat agaatgaacg tttgtaagag accgaatgtc tttctttccg
     1021 tacatagttg ccatcttctt tatcttctgt atgggactca tgattggcgg agatcgtcaa
     1081 agtattatct gaatattcaa ctgtcgtgtc ttctttgtca cagccaggca tatcagcagt
     1141 tagtttgtat tctttttcat tttcgactaa atctactttt ggataacttg aaacacctaa
     1201 aaaatcattg aatgctggac taaacacgtc attgaaatca gggaacatgt cgcgaatgct
     1261 aggtacattt gccatttttg atcatccttt caataagtat tttttattta tccctattta
     1321 cgtaataact ctattttgaa aaaaatgcaa gtataatggt tagctttgtg aatatttcat
     1381 ctaatacgat acaaaggcta tctttctaat ttgaaaaagg gatcattaaa ataataagaa
     1441 gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc aaatatagaa aaattgaatc
     1501 tagaaaaccg cccgacaata aatttcatta actcagttga ccgaaaatga agtagatacc
     1561 aaaaatttat caagagagca agtagagaag tgagtatatt cataagagaa atagctgtaa
     1621 cgaattatta ggttaatgca aatgcttatt tatactccgt ataactttct cagaaaaaat
     1681 gataccaata atgacttgct agtactgtaa atccgtataa tatcatctaa tcaaaatggt
     1741 gttccatttg catctgtcat cgtaatcgat ccttcgggtg acactcgata aaaaccaacg
     1801 cttccagatc ctccttggga acggatttgt ttgctggaag ctgtgaacaa ataatctcga
     1861 tcaatttttt gataaaagct aataacaata tcgtcacttt gtaattcgga acggtgggca
     1921 ataagaaata gaagtgcttt gtcagagttt tctaatggaa gagaaggtac agtgctttct
     1981 tgcatttcag atgttgaaga aatgctaaac gactgaatac ttgattggct atgttgcgaa
     2041 tctacattac cagtagaagt attagtaatg gaagtagtag aagtactttt agttgaacca
     2101 acctcagttg ttgaaatggc tgctgaagat tctttaacag aattctttgt tttatatcca
     2161 caggcagaaa aaagtattac agttcccaac aagcctgcgt aaatcccttt atgcatgata
     2221 gctcctccta aaatagatta tctttattca gtatatcact taaacaaaga aaagcagatg
     2281 gtttaaaatg aaaaaacctt cccaataaag ggaaggttca tgaagataat aactttttta
     2341 ataattatag ctttctaata tctttcatca acttttctag taatcattgg gaaaaatttc
     2401 aagcagtaga tcgcacaaat acctacgacg atataaatga tcttcgcaaa aatagttgtt
     2461 gaaccaccag caatcatagc tactaaatca agttcgaaca agcctaccaa cagccagttc
     2521 aaccctccca caatcaataa agttaatgca atagcatcta aagttttcat tagaattctc
     2581 ctttcttaaa tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc
     2641 aggaatttta gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac
     2701 attttttagc tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg
     2761 gatttgtaag aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga
     2821 aaagtagtaa ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg
     2881 ttgaagcaga atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa
     2941 aacctactat ctttaaaagt cgatgtgtct tctaaaacgg acataaaaga agggatatat
     3001 aaagtaatga aacattttaa gacgatcgat ggtctagtca ataatgcagg aataaatatt
     3061 ccttctcttc taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt
     3121 tttgataaga tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga
     3181 agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg
     3241 gaaggatcag aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact
     3301 cgttcttggg caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg
     3361 atactggaag aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga
     3421 ggtataagcg tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga
     3481 aggagtggta agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca
     3541 agttatatca ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata
     3601 ggggggacgc acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact
     3661 cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata
     3721 aaaaaagata ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa
     3781 aacaaacatt ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga
     3841 aaattgaaaa cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat
     3901 aggctgatcg aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc
     3961 agcagaggaa cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat
     4021 gtaaagatta caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta
     4081 cgactacttt tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt
     4141 ctagaagaaa aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa
     4201 atcacccttt taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg
     4261 ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa
     4321 ctgatcaatc agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt
     4381 atcagttttc ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct
     4441 gatttgcgct tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat
     4501 ctggatgagt ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg
     4561 gtaatttttc gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct
     4621 ttttcttacc aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag
     4681 atctctaaag tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa
     4741 aaagagcatt tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca
     4801 ttaatgatcc aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt
     4861 tcagaagaac aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt
     4921 ccgttaaaaa atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac
     4981 tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct
     5041 actgtttttt tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa
     5101 ttagtcgctc ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg
     5161 aaaagagaat atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc
     5221 aaccagctat ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca
     5281 gaaacaggag gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa
     5341 gttgagaatg aattatttga gctatacgat atcctatttg aactcataaa aatgggaata
     5401 gatgagttta ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa
     5461 ggagagagac ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt
     5521 gctgcaagca gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact
     5581 gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat
     5641 tgttatgcta gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt
     5701 tacatctttg gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat
     5761 cgatcgttac catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa
     5821 tgccagagaa gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt
     5881 gccttttatt gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag
     5941 gagtgacaaa cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa
     6001 acaggtgcag aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg
     6061 atggttgcaa tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc
     6121 aaattatcag cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt
     6181 atgttgggaa atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca
     6241 agctattatg ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt
     6301 aatcctggag aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta
     6361 aattcgatgg agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt
     6421 ggttggataa ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc
     6481 aaaaaagtag agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta
     6541 agatcgtaag aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata
     6601 aacacaagtt catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag
     6661 aagaactgac aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa
     6721 tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag
     6781 ggataccaac catcaatatc gttcccacag gaaaaagtgg cccattagca caatatatca
     6841 ctgaagatat ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg
     6901 atgataacaa agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt
     6961 ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg
     7021 gcgcgggaaa agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga
     7081 tccggacagt gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt
     7141 ctgggatcgg taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat
     7201 taacaatcat tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg
     7261 cagttattag ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc
     7321 ctccccaatt agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta
     7381 ttccagtcgg tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat
     7441 cagttcttta ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa
     7501 gttttggatt atatagctg
//
LOCUS       DAJSBC010000108         7509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSBC010000108 DAJSBC010000000
VERSION     DAJSBC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.110"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(190..867)
                     /gene="nth"
                     /locus_tag="ONT65_002560"
     CDS             complement(190..867)
                     /gene="nth"
                     /locus_tag="ONT65_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR2330148.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(874..1572)
                     /locus_tag="ONT65_002561"
     CDS             complement(874..1572)
                     /locus_tag="ONT65_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2330149.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1840..3024
                     /locus_tag="ONT65_002562"
     CDS             1840..3024
                     /locus_tag="ONT65_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR2330150.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3030..3473)
                     /locus_tag="ONT65_002563"
     CDS             complement(3030..3473)
                     /locus_tag="ONT65_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2330151.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3699..3986)
                     /locus_tag="ONT65_002564"
     CDS             complement(3699..3986)
                     /locus_tag="ONT65_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330152.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4157..5509)
                     /locus_tag="ONT65_002565"
     CDS             complement(4157..5509)
                     /locus_tag="ONT65_002565"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR2330153.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5782..6228)
                     /locus_tag="ONT65_002566"
     CDS             complement(5782..6228)
                     /locus_tag="ONT65_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2330154.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6464..7390)
                     /gene="cysK"
                     /locus_tag="ONT65_002567"
     CDS             complement(6464..7390)
                     /gene="cysK"
                     /locus_tag="ONT65_002567"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR2330155.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2026 a         1542 c         1232 g         2709 t
ORIGIN      
        1 tttctacatc gggtattttc ccaataaaaa taacaacttt tatttttatc gcaataaatt
       61 gtaataagtt accatcctag ctactgagta tgtacattta ctttttcatg caaaaaagat
      121 aaacgaaaca gcttttctcc ataataacaa gataattcat acatttattt gcaattaaag
      181 aaaccacgtc tacaggcgtg gtttcttttt tagcgccttt tcttttagac gcatccgatt
      241 tttcccatct tgacatattg atagcaaagg acatacctcg cacttgggat ttcttgccgt
      301 gcaatgatat ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata attcttgtgg
      361 tactttccgc atcagtgttt cttctacttc catgaccgta gcatctagtt tgcagatcct
      421 caggcgtttt gagacacgtt ccacgtgggt atctactgcg attgctggta tcccgaatgc
      481 atcccccaat acgacatttg ctgtctttct gcctacacca ggaagagaca tcaattcttc
      541 tcttgacgtt ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac acgctttgat
      601 gttttttgct ttatttcgat acaaaccaat cgtttttatt ttaagaatga tttcatcgat
      661 tgaagcttct gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt
      721 attcactgat acatctgtcg cctgggcact taagatcact gcaatcaata actcaaatgg
      781 attattgtgc ttgagttctc catgtgcttc gggaaacatt ccatacatcg tttctagagc
      841 ttccattgtt ttttgtttgc ttaacatatt ccgctactcc tccaaccaat ttttcagtga
      901 gactttcggc aattcttctt gttgagctga ctgttctact tctctttgaa gaagctgctg
      961 ttttcttcgt ttttggtctt cttccacttg ttgttttgta cgtagatttt ttctttccca
     1021 cgatagcaaa atgcggtcaa cgtaattaaa actataagcc tgatttaata cggcttcccg
     1081 cagtgctagc ttcaaaattt ccggctgata atggtcttct tctagccatt gtccgatccg
     1141 ctggaactcg atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt gatacactga
     1201 ctggatttct ttttcttgtg ttttttcttc gtattttttt tcttgtgttt ttaaatactc
     1261 acctaataga tcatagatgg ggtaaagatt atatcgatct gcttttttac cgttgacaat
     1321 cgtcgtttct atcttgataa atccatttgt tacgagacga tccagtattt gaaaaatctg
     1381 atcttgtttg attcccatat ccatagcaat catctgcaaa tcagggaaag tatctccttc
     1441 aagctgtgcc atatgcaact gtagaacaaa taaaaattct tcagattgta gaccgatcct
     1501 atgataattt ttaagtaaaa gattagatat gactgtttgt ccagcgctta agtaattttc
     1561 taaattcaac atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag
     1621 caagaacagg tttcttttct atttacttca ctgatcggtc agatttacaa tgtcgtctac
     1681 ttgatttttg tttgttcatg tttttgatgc gtatcttcac ttttcggaat aaataaatta
     1741 agtagatttc gctgttcctt agaatgttga caaggaaaca aaattactat acactatttt
     1801 tgtttccaag ttaacattat tgcataaagg agtttttaca tgtacagttt cacaaataaa
     1861 cattctaaag tttttggata cgtctctgtc ttattcactg gattaggatt tactatcgtc
     1921 agtcctgtac ttccgttttt aacgctgcct tattctcact ccatacatca gcaagctttc
     1981 tatatcactt tgctcatgtc tgtttatgct ctagctgcct tcttatctgc ccccattctc
     2041 ggtagcttaa gtgatcattt tgggcgtcat ccgatcctta ttatcagttt attaggatca
     2101 agtatcggat atttgctttt tggtttagga aattcgatat ggatgttgtt ccttggtcgt
     2161 atcattgaag gattgacggc aggagaaatc tccaccttat atgcttactt tgccgacatc
     2221 acggaaccta atgaaagaac caaagttttt ggatggatgg gtgctttagt cggtatagga
     2281 acaacgctgg gtcctattat agggggtcta ttagctgagt tggggaattc tgttcctata
     2341 tttattggag cactctttac tttcttaaat gccgtttatg gctacacatt tatgctagaa
     2401 agccttccaa taaaaaaacg ttcagtagat ttaagtttct cccatgtaag accttttcac
     2461 caattacaac aattattcaa gatttcatcc gttattcctt tattgacagc aggttttgct
     2521 gtttggttgg cagctggctc tcttcaatct atcttttctc aattttcgat tgacgccttt
     2581 cagtggaagg ctgggttggt tggtttaagc ttctctttga ttggaatctt ggatactgtc
     2641 tcgcaattgt tcattatgcc tcgattgctc aaaaaatttt cagaacagca gatcacaaga
     2701 atagggatgt tcagtgaaat cctcgcctat ctattcatca cattatctgg aattcttcta
     2761 ctgccgattt tgtttttatt tgggattatc tgttatggtt tcggtgattc tatttttact
     2821 cctgtattca atggccaact atccaattct gtctcagata accagcaagg tcttgtaatg
     2881 ggcggtaccc aaagtataca atctctatcg cgcgtcatcg gcccgctaat cgcaggacag
     2941 ctttatgctg cagcaccatt tcttccgacg gtattcggat ttttgcttat gatttttgcc
     3001 tattatcact acaataaaaa atagtagctt catttgctac ttcgtataaa ttctctgtat
     3061 cgaccgataa aacgaaatac tgtgtcaaat tcttcttttg acatattttc aaatacagta
     3121 gcatctcgct gtacccattt ttgatggagt tgctcgtgtc tattccttat tttttgtccg
     3181 attggcgtca attgaaaata gatttctttt ttattctctt cgctttgata agtgtttatg
     3241 atccctctag ctatcagttt ttttgttagc ttactgatgg ctcctctagt catgtaacta
     3301 gcattcgcta attttgtcac gttaggatca gggattttcc cgatatgttc aatcacttct
     3361 agttcattta gagtaaatcc tgcaaattct ttttccattt ctggtttatt acgatgaata
     3421 atctcattag ctaaatccag atattcttct attagcagtt gtcttttatc catttctaca
     3481 ccttcttttt ctttcattat attgtttttt tgtttcctat tcaacaaaga taaaaatatt
     3541 ttgtaggaaa acccaatctg aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc
     3601 cctttttctg attaaaagat agtaaagaga cagtcatcgg taattgtgat gattttttgt
     3661 catcacttaa ggatgagcgg ccttttcaaa aactgtcttt aaagatcatc atcagtttct
     3721 agcacatatg gctgaccttc atatgtgtac tctgtattca atgtgccgtc attttcatct
     3781 gagtagatgc gtctactgaa aggaaaacga tgagaaacat tatctttggc gtcatttgct
     3841 ggcatcgtgt aatattcctc ttgcgtttca ttcaactgag agagtacttg atctgccaac
     3901 tcatcaatat tgatcgttaa tttcttcgaa gcacttttat aatcttctaa aaatgcatct
     3961 tgttggacaa aatattcttt attcatcttc tttctcacct ttcttttcaa aatcaaataa
     4021 agctgcttgt tcttttatgt aacagcttta tctttgctgc ttattgtgac aaaaaattcg
     4081 aagaaagaca actaattgta cttttcacaa aaaaagacag agggtcgtga caaaattgtg
     4141 tcacaaccct ttttctttat tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta
     4201 ggttcaagta attccatggg cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga
     4261 gatcttctac agtcatttta ttttgaacag ctaaagacat ggtatttgct gattgtgtaa
     4321 tgtcgtattt ggacatgaac tgagcaccta cgatacgatt cgttcctttt tcatagacta
     4381 gctccatcat tactttttca gtagttggca tgaactctgg acgataatta tcttcaaata
     4441 cagtcgcttg gacttctaga tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac
     4501 cgatcgtcca gccaaatagg taaagtcctg aagttccttg agtaccacga tacgccattt
     4561 tatgatctgt caagttattt cccacaagca aaccttgacg aacggcattt gttgccaaag
     4621 gaatatagtt ttgcgtgcca cttggattgt agttgactac tgcagagtct ccagctgcaa
     4681 aaatgtctgg attacttgtc tgcatgtatt cattcacttt gattgccccg tttggcagtg
     4741 tatctacttt tccttcgatc agttttgtat ttggacggaa gcccacacat aaaatgacca
     4801 tatctgtatc aaatgtatct gttggtgtag ctacttgttt cacttttcct tcttcatctg
     4861 tgataaattc agtaacattt tcgcctagcg caagagtaac accgcggtct gtcaattctt
     4921 tttctaagat atctgtgaat ggtttatcta gatatttatt caagatgcga tctaatccat
     4981 caatcaatgt cacttgtttt ccagattcga caaaagcttc tactaattca ataccgatat
     5041 atccaccacc aacaattact acacgttttg cttctttcgc ttgtgcaata atttcatttg
     5101 cttgattgta gtttttgcat aggagtacat ttttcgcatc gattccacta atcggtggga
     5161 taataggcca tgaaccagtt gtcatcacta atttatcata agaaaccgtt tctgtttctc
     5221 ctgttttcag atcttttgcc gttactgttt tattttctgt atcgacgtct gttacatcgt
     5281 gttccatttt aactgttgct cctaatgaag ataattcttc aggatttgag tagaacaagc
     5341 ctgctgggtc ctttactact ccgccgacat aaagtgcgat accgcaagat aagaaagaaa
     5401 tattgtcatt tctttcatat actgtcactt ctgcttctgg atgatttttt aaaatacttt
     5461 ttacagctgc agtacctgca tgagtacagc caattactac tactttcatt tatggtccct
     5521 ccgattaaca ttttgtcctt cgacaaaact actataacag aaataaaacg gatacaacag
     5581 tttaatgctt gtgaattcac aaaggtttgt ttgtagtttc attccttttc aagcataaga
     5641 acccaatatc tacaagattc ttattttaat aatattcact tttatatcta agattgtgaa
     5701 taaaagacaa aaaaataaaa aagctagcgt tttttttaca cagctagctt tttttaggta
     5761 taacacttgt agaaactgtt tttacatttg cgcctttttt gctgactctt tacaatccgg
     5821 acagagtcca tacaattcca accgatgttc attaatctca aaatcagtca gtctgcttgc
     5881 agccatttct atatcttcaa gccctgggta atgaaaatca acaatcttac cgcagttttc
     5941 acaaattaca tgataatgac gctttgaact gaaatcaaat cggctagaag catcgccgta
     6001 cttcatttct tgaacaaatc caatcgaagt aaataagcga aggttattgt aaacagtcgc
     6061 aacactcata tttggaaacc gatgttcaag tgctttataa atctcatcag ccgttggatg
     6121 ggaatgatgt tcaatcaaat attctaaaac cgcgtaccgt tgaggtgtga ttcgaatgtt
     6181 ctcttttttt aacttttcga taacctcttc gactaactta ttttccatgt aaacacccca
     6241 cgttgttatt ctcgtcttat catactattt ttacatttta aaatccactt aacatgaaaa
     6301 gaaaattcaa ttttattgta ttttaatgaa aaaagacgta aaacagtcgc gttcactatt
     6361 ctacgtctct attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt
     6421 aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa
     6481 gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt
     6541 ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta
     6601 ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa
     6661 tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga
     6721 ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt
     6781 ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga
     6841 tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt
     6901 gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt
     6961 gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag
     7021 aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac
     7081 gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc
     7141 ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg
     7201 gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta
     7261 cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta
     7321 cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga
     7381 tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg
     7441 ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat
     7501 atggtaatt
//
LOCUS       DAJSBC010000109         7387 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSBC010000109 DAJSBC010000000
VERSION     DAJSBC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.111"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            123..515
                     /locus_tag="ONT65_002568"
     CDS             123..515
                     /locus_tag="ONT65_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330156.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="ONT65_002569"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="ONT65_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="ONT65_002570"
     CDS             857..1054
                     /locus_tag="ONT65_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330157.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="ONT65_002571"
     CDS             complement(1097..1672)
                     /locus_tag="ONT65_002571"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2330158.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="ONT65_002572"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="ONT65_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="ONT65_002573"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="ONT65_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HCR2330159.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="ONT65_002574"
     CDS             4334..5734
                     /locus_tag="ONT65_002574"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR2330160.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="ONT65_002575"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="ONT65_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2332 a         1228 c         1311 g         2516 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtagaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     7381 taccaat
//
LOCUS       DAJSBC010000110         6898 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSBC010000110 DAJSBC010000000
VERSION     DAJSBC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.112"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..182)
                     /locus_tag="ONT65_002576"
     CDS             complement(<1..182)
                     /locus_tag="ONT65_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2330161.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ONT65_002577"
     CDS             517..951
                     /locus_tag="ONT65_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2330162.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ONT65_002578"
     CDS             974..1294
                     /locus_tag="ONT65_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330163.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ONT65_002579"
     CDS             1435..1626
                     /locus_tag="ONT65_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330164.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ONT65_002580"
     CDS             complement(1697..1954)
                     /locus_tag="ONT65_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR2330165.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ONT65_002581"
     CDS             2112..2375
                     /locus_tag="ONT65_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330166.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ONT65_002582"
     CDS             2399..2587
                     /locus_tag="ONT65_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330167.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ONT65_002583"
     CDS             complement(2670..2855)
                     /locus_tag="ONT65_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330168.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ONT65_002584"
     CDS             3070..3441
                     /locus_tag="ONT65_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2330169.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ONT65_002585"
     tRNA            complement(3591..3663)
                     /locus_tag="ONT65_002585"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ONT65_002586"
     CDS             complement(3709..4137)
                     /locus_tag="ONT65_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330170.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ONT65_002587"
     CDS             complement(4197..4955)
                     /locus_tag="ONT65_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR2330171.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ONT65_002588"
     CDS             5250..5447
                     /locus_tag="ONT65_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330172.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ONT65_002589"
     CDS             5553..6035
                     /locus_tag="ONT65_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330173.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ONT65_002590"
     CDS             complement(6093..6689)
                     /locus_tag="ONT65_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2330174.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2335 a         1060 c         1355 g         2148 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaa
//
LOCUS       DAJSBC010000111         6375 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSBC010000111 DAJSBC010000000
VERSION     DAJSBC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.113"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            37..1059
                     /locus_tag="ONT65_002591"
     CDS             37..1059
                     /locus_tag="ONT65_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2330175.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1070..3274
                     /locus_tag="ONT65_002592"
     CDS             1070..3274
                     /locus_tag="ONT65_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2330176.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3295..3444
                     /locus_tag="ONT65_002593"
     CDS             3295..3444
                     /locus_tag="ONT65_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330177.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            3520..4113
                     /locus_tag="ONT65_002594"
     CDS             3520..4113
                     /locus_tag="ONT65_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2330178.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4129..5028
                     /locus_tag="ONT65_002595"
     CDS             4129..5028
                     /locus_tag="ONT65_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2330179.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5031..5516
                     /gene="ssb"
                     /locus_tag="ONT65_002596"
     CDS             5031..5516
                     /gene="ssb"
                     /locus_tag="ONT65_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2330180.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5882..6142
                     /locus_tag="ONT65_002597"
     CDS             5882..6142
                     /locus_tag="ONT65_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330181.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2324 a         1097 c         1317 g         1637 t
ORIGIN      
        1 ccagagtggc tgattgatta tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa
       61 catgaacggc aaaatttctt agttcgagac gatcgtttag atcatgcggt cgccttttta
      121 tggaaagatc cacagacaaa tgagacagta ggggcgagtt atcaaggaac gttcattgat
      181 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
      241 catggcttta acctaaagat cggtgaccct aagcagttaa aattttttga gagtagtatt
      301 gatttactta gctatgctgc actaaatcgt gatcagttaa acgatacgtg gttagtttcg
      361 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
      421 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
      481 catattttct acgaaaaaga acaattaatg ggcgcagttg atccatttac gaatcagaaa
      541 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caaaagaata caaagtcatt
      601 tatgaagaag tcgcaaaaga agaaaagatc acaccagaag ctattatggc catacacaag
      661 acagaaaaca atctgcaatt aacgaaccaa ttggtttccg ctcacaaagt aaatgcgtct
      721 tttggtcagc agctttctgt taacgattca atagaagcta ttaatttgaa agatatttgt
      781 agagaagtcg caaaagaatt aaaagcttgt gaacgcgtgg atggtacgta cgattttgat
      841 cgtttttatc aagaaaaagg ggatattaat gctcagattc ttttctctta taaagcagaa
      901 caatactaca aaggctataa aaatcatgaa catgaatttg tacctgaagt aaaaaaggat
      961 tggaatgatc aattgaagca tgaaatctac caacaagaga tcagaaagca aaaacgtgcc
     1021 atgttgtttc aacaaggtag acagcaagaa agggagtgaa atcgtcatga tgaaaaccgt
     1081 tattttagca gaaaaaccgt cacaagcgaa ggcttatgcc gatagctttt ctaaagctac
     1141 ccgaaaagat gggtattttg agatccaaga tcgattgttt tcaggagaaa ctgtgatcac
     1201 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaattggaa
     1261 acaatggtca ttagagcatt tgcctatttt ccctacacat tatcactatc atgttcctaa
     1321 agataagaaa aagcaattca aggtggtgaa acagcaactt caatcggccg acaccattat
     1381 tattgcgaca gatagcgatc gtgaagggga actgatagct tggacgatca ttcaacaagc
     1441 aggcgccgat catggaaaga tatttaaaag actttggatc aactccttag aaaaagaagc
     1501 catttatcag ggattccagc agttacgaga tgcagaagag acctacccaa aatttgaaga
     1561 agcacaagca cgccagatcg ccgattggtt gatcggcatg aacggtagcc cgctgtatag
     1621 tttactcttg cagcaaaaag gcataccagg aagtttttcc ttaggcagag tccaaacccc
     1681 aacgctttac atgatttatc aattacagga aaagattaga aacttccaaa aagaaccata
     1741 ttttgaagga aaagcccaag taatcgctca aaatggcgca tttgatgcga agcttgatcc
     1801 gaatgaaacc caagcgacgc aagaagcatt tgaagattat ttaaaagaaa agggggttca
     1861 attgggaaaa cagccaggta cgatccacca agttgaaacc gagaaaaaaa gtgcggcaag
     1921 tccacgtctt ttttctctat ccagccttca atctaaaatg aatcagctca tgaaagctag
     1981 tgccaaagat actttggaag ccatgcaagg gttgtatgaa gggaaatatc taagctaccc
     2041 acgaacagat accccttata ttacagaagg ggagtatgcg tatctgttgg atcacttaga
     2101 cgaatacaaa cactttttaa aggccgaagc cattcctact cctattcata caccgaatag
     2161 tcgatatgtg aataataaga aagtccagga acattacgcg atcattccta caaagacggt
     2221 catgacggca gcggcttttg aacaattaag tccactgcag caggctattt atgaacaagt
     2281 gttaaaaaca acggtggcca tgtttgcgga aaagtatacc tatgaagaaa caacgatctt
     2341 gacgcaagtt cagcagcttc agctaaagac agtcggaaaa gtaccagtag atctaggttg
     2401 gaaaaagctg tttggcaaag agagcgacag aaaagaaaag gaagaagaac cgctacttcc
     2461 taaagtgaca aaaggagaag cggtgacggt agatctacaa gtccttgaaa aagaaacgaa
     2521 gccaccacaa ccttataccg aaggtacgtt gatcacagcg atgaaaacag ctggaaaaac
     2581 cgtggatagc gaagaagcac aatccatatt gaaagaagta gaaggaatcg gtacagaagc
     2641 aacacgagca aatattattg aaactttgaa acaaaaggaa tatatcaagg tagaaaaaaa
     2701 taagcttgtg gtgacaaata aagggatttt actttgccaa gccgtggaaa aagagccgct
     2761 gttaacaagt gccgaaatga ccgccaaatg ggaaagctat cttacaaaga ttggtgagca
     2821 aaaagggaca caagctactt ttctagcaaa tatccaaaaa tttgtttccc acttgttgga
     2881 agtcgtccca ggacaaatcc aagccactga ttttggagca acgttacaag aggtgaaagt
     2941 agccagtgaa aagcaagaag cggcgcgtca tttaggatct tgtccaaagt gccaggaaaa
     3001 agaagtcata ctttatcaga aggtggcgac ttgcacgtct gaagcctgtg attttaaatt
     3061 gtgggtaacg attgccaaaa agaaactcac agcgacacaa ctgaaagaga tcattcagaa
     3121 cggccgaaca agtcaaccag tcagaggact aaaaggtcag aaaggttcgt ttgaagcaac
     3181 gatcgtttta aaagaagact ttacaacagg atttgagttt tctgaaaaga agaagaccac
     3241 ctacaaaaga aaagtgagac gtaaaacgag ttagaattga cggagggaaa aacgatgaga
     3301 aatacaacgc ataaagaaca agaaatcgaa aatcaaaaag ccaaaatacg ttcagagcag
     3361 gagaaaatgc gtgccagcaa acgaagacta cagcaattga agaatcaaca acggattcaa
     3421 aaagaacttg aattagatcg ataaatcctc ctgttgttaa tattaaacta acatgttata
     3481 gttatgttag tttaatatta acattcgaga gggaaagcga tgaaagataa agtcaagtgc
     3541 atttttgaga aatacaatgg taatctatcc ttgaaggatg ctagaaatga agggttatca
     3601 cctatgacgt tagatcgttt agccgaaaag gacaaaatag aaaaaatagc atcaggtttt
     3661 tataccttaa aagatagttt tgtggatgaa ttatatctcg ctcaaagtat ctatactaga
     3721 ggaatcttct ctcacgagac agcgcttgat ctttttcaag taggaacata tatccctaaa
     3781 aaaattaata tgatgtttcc taaaggatac aatgtttcga aagagaattt agatcagtat
     3841 gccattcaac cgcattacac gaataaagaa aactttgaac tgggaatcac agaggcagaa
     3901 agcttttacg gcaataaaat tttcgtctat gatttagagc gaacgttatg tgatatgtgg
     3961 aatcccagat ataaagcaag cgtagaagta aaacaagaag cattaaaaga atatatgaca
     4021 agtaccagca gaaatacaaa taagctaaga agatacatga atattttaaa atcaccaaaa
     4081 gaaatgactt tgtatatgtt gccactttat tgagaggagt aaaggtgaat gacaccgaca
     4141 caatttaaag cgaaaacaag aaatgttgca gctgagaaac atttaaaccc acaaattgtt
     4201 caaagatatt atatgatgga aaaattatta gaatgtattg ttgaatcgcc ttatcatgat
     4261 gattttgtat taaaaggggg atttctgatc ggatcaaaat acgggttaga aaaccgttca
     4321 accgttgata ttgatacgac ttatcgaaat tcgaaactaa caaaagaaaa attagaaact
     4381 gttttttctg aattaacagc tgctcctaca aaagatggca ttcggtttga attaaagggc
     4441 ttaacagaga cgagggaagc cgattattac ccaggttttc aagcaaagtt tattgctttt
     4501 cttgaaaatg cacgtgtacc ttttaaatta gatattactt caggtgattc tattattcca
     4561 gcagcattga tatatgaaca taaattgatg tttgaagata aaacaattca tattccagct
     4621 tatcctacgg aacaaatttt agctgaaaaa ttacatgcca cgtttagttt cggaatagat
     4681 aattcacgta tgaaagattt ctatgatctg tatatgatcc ctaaactaga acgtatcgat
     4741 gagataaatt tgtataagag tgttcaagga acatttaagg aaagaaataa tcaatcagtg
     4801 tttaagaatt tgtataatca ggaaatgcct caattgatga agaatgaggg attgagggag
     4861 aaatggaaaa gatatcagca agaaaactat tttgccaaag atatttcttt cgaatctacc
     4921 ttaacttctg ttgattcgct gatgcaatca attgtcgagc aagaggaagt agaacgaaaa
     4981 caaaatttta agagatcaca acaaatgaat caagaaatgg agagataaag atgatcaata
     5041 atgtaacttt agtaggtcga ttaaccaaag atcccgattt acgttacacc gcaagtggca
     5101 ctgcagtgac cacttttacc ttagccgtta accgtaattt tactaatcaa agtggcaatc
     5161 gagaagctga ctttatcaat tgtgtgattt ggcgcaaacc agctgaaacg atagcgacat
     5221 tagctaaaaa aggcagcttg atcggcgtcg tcggacggat tcaaacgcgg acttatgaca
     5281 accagcaagg acagcgtgtc tatgtgactg aagttgtggc cgataatttc caactattag
     5341 aaagtaaagc agcgacagaa agccgtatgc acgctgatca atcgtccagc agtccaagcc
     5401 ctaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
     5461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttctaagtag
     5521 cattttctat tggttgcatt tgctacggtt tatgttataa tttagacaac aaaaagaatt
     5581 ttcaatctat aaccaaaaac cactagctgg cgggctagtg gctttctttt acgtttctat
     5641 tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc aagccatttg
     5701 cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc
     5761 aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta
     5821 tagcataatt gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt
     5881 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt
     5941 ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga
     6001 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     6061 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
     6121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
     6181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     6241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     6301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
     6361 tctcaccaaa aggtt
//
LOCUS       DAJSBC010000112         6326 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSBC010000112 DAJSBC010000000
VERSION     DAJSBC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.114"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(207..1169)
                     /locus_tag="ONT65_002598"
     CDS             complement(207..1169)
                     /locus_tag="ONT65_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2330182.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ONT65_002599"
     CDS             complement(1171..2610)
                     /locus_tag="ONT65_002599"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR2330183.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ONT65_002600"
     CDS             2795..4741
                     /locus_tag="ONT65_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR2330184.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="ONT65_002601"
     CDS             5005..5877
                     /locus_tag="ONT65_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2330185.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6173..>6326
                     /locus_tag="ONT65_002602"
     CDS             6173..>6326
                     /locus_tag="ONT65_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2330186.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTA"
BASE COUNT         2022 a         1098 c         1115 g         2091 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta
     6181 tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc
     6241 ggttgctatc gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa
     6301 ctttctcaaa tgtggtggaa cagcac
//
LOCUS       DAJSBC010000113         6027 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSBC010000113 DAJSBC010000000
VERSION     DAJSBC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.115"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1039
                     /locus_tag="ONT65_002603"
     CDS             <1..1039
                     /locus_tag="ONT65_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCR2330187.1"
                     /translation="QFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIG
                     FFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINST
                     ADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGN
                     TFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPI
                     LSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGK
                     DIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCYRGRNI
                     ISMIKSREHKR"
     gene            1075..1791
                     /locus_tag="ONT65_002604"
     CDS             1075..1791
                     /locus_tag="ONT65_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2330188.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1810..3393
                     /locus_tag="ONT65_002605"
     CDS             1810..3393
                     /locus_tag="ONT65_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HCR2330189.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3528..4106
                     /locus_tag="ONT65_002606"
     CDS             3528..4106
                     /locus_tag="ONT65_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330190.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            4052..4570
                     /locus_tag="ONT65_002607"
     CDS             4052..4570
                     /locus_tag="ONT65_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330191.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            4578..5618
                     /locus_tag="ONT65_002608"
     CDS             4578..5618
                     /locus_tag="ONT65_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2330192.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            5867..>6027
                     /locus_tag="ONT65_002609"
     CDS             5867..>6027
                     /locus_tag="ONT65_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2330193.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2184 a          692 c         1170 g         1981 t
ORIGIN      
        1 tcaatttcta atacaatttg ctaaagggtt agaaaaagtt aaagctatgg gcatagcagg
       61 tattatcagc acaattgtaa caataattgc caatattttc ttcttgctta ttctgaaatg
      121 gggattaatt ggttttttca ttgcaaatat tttatcacag attatttcag ctacttatct
      181 gtcagtatta gtgagaatat gggaatatat cgattttgag gctaaagatc ctcagctaaa
      241 gagagaaatg ctaaattact cgattccatt aattgcaact gttgttggat ggtggattaa
      301 tagtacagct gataggtatg tggtttcctt tatagtaggt gtctcggcga atggcttatt
      361 gtctgtggca tataaaatac ctcaaataat taatactttg cagggaatat ttatacaggc
      421 ctggcaaatt tctgcaatta aagaatatgg agataatgag actgcaaaat tttatggaaa
      481 tacatttgtt atgattaatt tgttaatgtg cataacctgt tcaagtctta tcatattaac
      541 tagaccgttt tcacatattc tttatgcgaa agacttttat gaagcgtgga aatatgttcc
      601 atttttatta atagctaatg ttttaaattg tgcatcaggt ctgttaggac ctatcttatc
      661 agcaaaaaaa gattctaaat ccatgatgtg gtctgcaatc ataggggcat tagttaatgt
      721 gattatgagt gtagtccttg tttataagat aggggttcaa ggtgccacaa tagccactgt
      781 tatatgttca tatgtaatat atgttgttcg taaacgagct gtgggaaaag atatttttat
      841 aaagcattat catattgttg tggaatcttg gggattattg tttgttcagg caatcataga
      901 aatatatttt aaaaattatg taatagaaat tataattatg attattttaa ttattataaa
      961 tatcagttat attaagcagt tgtgttatag aggaagaaac attatttcaa tgattaagag
     1021 tcgagagcac aaacggtaga cgatgttatt tttataaaaa gatataattt attaatggtc
     1081 gataattatg aaaggaaaaa tattcttaaa aaaaagagta taggatgtcg tatgaaaata
     1141 gtcgctatag taccaatgaa gttaaataac aaaagattgc cacaaaaaaa cacgaaagct
     1201 tttaccaatg gaaaaccatt gtgttattat atactttcaa cacttttaaa agtaaaacag
     1261 attgataaag tgtatgtcta ttgtagtaac ccagatatta aagaatttat accagaaggt
     1321 gtaacatttt taaagagatc tagttcctta gatcaggata caacgagtat tacacaggta
     1381 ttagccggtt tcacaaaaga agtagaggct gatgtatatg taatgacaca cacaacagca
     1441 ccatttatta gcgaaaaatc tattgaaaaa ggactagatg ccgtgctgac aggagaatat
     1501 gattctgcat ttgctgcaaa aaaattgcaa gatttcttat ggaaagacgg tgaaccattt
     1561 aattatgaat tggacaatat tcctagaaca caggatttgc cgcctttata cgaagaaact
     1621 agtggatttt atatttataa tcgcaatgtg atgaccgagt tgcatagaag aattgggaaa
     1681 aaaccttgca tagttgaagt aggtgagatt gagtcagttg atattgatga agccgaggat
     1741 tttatgattg ctgatgcgat ttataatcat cttattttac aaggtagtta agacaggggg
     1801 aaggttagga tgaacaatgt acagatttta gattgcacac ttagagatgg gggctattgt
     1861 aacaattggg aatttggatt tgataataca aaaaaaatta tttctggact tatagaatca
     1921 ggaattgaga ttatagaatg tgggtttctc acaaataaaa cagtatacaa taaaaatgtt
     1981 acaaaatata cgaccttaga agaattagca cagataattc ctgaaaaaag agaaggaaaa
     2041 ttatttgtag caatgatgaa ctatggcgaa tataatatag aggatcttcc agattatgat
     2101 ggttcatcta ttgatggaat taggattgcg tttcataaaa aaaattgcaa tgaagcgttg
     2161 aaattatgtc gtgagataaa gaaaaaaggc tacttagttt ttgttcaagc tatggtatct
     2221 ctcaattata cagatgaaga gtttttagca gtaataaaaa atgtgaatca gtttaaacca
     2281 tacgcttttt atattgttga tagttttgga atgatgaaag gaaaagattt aactcgtctt
     2341 ttttacatga ttgagcataa tctagaaaaa gatatacaga taggttttca ctcacataat
     2401 aacatgcagc ttgcatattc taatgcacaa aaacttataa ccgtacaaac aaatcgaagc
     2461 ctgattattg attcttcggt gtacggaatg ggaagaggag caggtaatct taacacagaa
     2521 ctatttgtag aatatttgaa tgataatgca ggaaaaaaat atcaattaaa accattatta
     2581 tatataattg atgaaatact aaatagtttc tatcagaaga gttattgggg atattcatta
     2641 ccaaactata tttcagcagt tcataatgct catcctaatt atgcaagcta tctgtcatat
     2701 aagaatactt taacaattga agcaatgaat gaaatattcg aaatgatgga tgaagaaaaa
     2761 aaggtatctt atgataaaca atatatagaa gatttatact acaaatatat ggaaactggg
     2821 aaaatacagg aggaatatat ggaagaactg agaaaatttc tctcagacaa aaaaattcta
     2881 ctgattgcac caggaaaaag ttctgtagat gaggtagaaa gaataaaaga gtttgttacc
     2941 aaagaagaag tcgtaataat tagtgtaaat tttgaatatt ctcatataga tatagattac
     3001 gtatttttaa gtaatttaag acgttttcat gaattaccga aagaaagcag aactaaatgt
     3061 attgttacat caaatataca agcatcggat gtatatttac ggacgagtta taaaaagttg
     3121 ttatctgaaa gggaagttgt aagagacaat gcaggattaa tggcaattag atttttagcc
     3181 gatatgaatg tggatgaaat attcttagct ggatttgatg gatattcaca taatgaagat
     3241 gagaattatg gtgagcaatc catggaaatt ataacaaaat ttgcattatt agatgcaatg
     3301 aacgtgcaaa tgagagatga attgtcagaa ttagctaaaa aagtaaaggt gacattttta
     3361 acggatccac gttgggttcg aattgataag tagtagtaat aatgttttta aaattttgat
     3421 aaagagatag tgagagaata aaaatgggta aagtcttaaa taaaaaagaa gaaaaatatt
     3481 taagggacga tggggcgaga tgaaactcta attttgaact tttaagaatc atttctatat
     3541 tgtgtattgt tgcttaccat gctaatttgc gtttaaatta taatgcagac ggaattatag
     3601 tacatggttc attaactgct aaccagattt tcttgaatta tattggtatt tggggtcagc
     3661 taggagttaa ttgttttata tttatttcgg catatttttt aagtgagaaa agaaacattc
     3721 actttgaaaa tgttttggga attgtattag aatgtatttt ttatagtgta ttagtttatg
     3781 gaattctttg tgcatgtgga atagaaacat tttcattgaa cagctttgta aaagaaatat
     3841 tatcatttgc tctaggtaga tattggttta ttacatccta tttagcattg tatattctaa
     3901 gtccgtattt aaatatcatg cttaacaatc tggaaaaact tcagttgcaa aaaataagta
     3961 tgttattgtt tataggtgta tgctgttatc gtatgttatt taaaacatct ttgtttgggg
     4021 attgtgctgt tttaattgca ctatatgtca tatgttatac actgaaaaaa acgataataa
     4081 tatttttgag agacacagaa tcataggaac aattgtatgt ataacagtta taccacttct
     4141 catatccata ggaatacttt tcaaatttat accaaatgca atggttaacc agatggttca
     4201 tattggttct ccattgatgg ttatagcaag tatattttta ttttatgtat ttaaaaattt
     4261 ttcattttac agtaagtata taaataaact tgcgagaacc agtttagggg tatatatttt
     4321 acatgaaaat cattatttca ttgatggtat gaagtctaga ggcttaatat gggatgggat
     4381 atttggatta ggcaaaaggt ttttagatag ggatttttgg atttggttta taattgcagt
     4441 tgtagttgtg tgcataattt gcatggctat cgatgttgta agagaaaaat ttgaaagaca
     4501 gatttttaaa attaagagtg taaagaacct gtgtaaactt atgaatcatg ttataaatat
     4561 atacccataa ttaataaatg aggagaaaag aagtgaaagt tatatcattc ggagaattat
     4621 tattgagact tgcagcaccc ggttattcaa agttatttca aagagatagt ttggagacta
     4681 cattctgcgg aggggaagct aatgtagcgg tatccttagc aatatttgga tatcaatcag
     4741 aatttataac aaagcttccg gattcagatg ttggatatgc ggcagctaat gctttgcgtt
     4801 attttggagt aaatgttgat aatattatat ttggagaggg acgcatggga ctgtattatt
     4861 tagaaaaagg agcagctcag agaccatcta aagtaattta tgacagaaaa ttttctgcaa
     4921 tttcgttagc tgatagtgat gaatttaagt ggaaggagat cctaaacgat gcaaattggt
     4981 ttcattggac agggataaat ccagcattgt ctgagaatct ctttgagacg tccttagatg
     5041 catgtcaggc tgccaaaaaa agaggtataa caatttcttg tgatttaaat tatcgatcca
     5101 agctttggac atcagaggaa gcaaaagaaa agatggaaca gatactacca ttggttgata
     5161 tttgtatttg taatgaagaa gatgcagaaa aagtgctagg aattaaagct gagcatacaa
     5221 atattaatag tggagaatta aataaagatg gctatgtgca ggtggctagg gagattcaca
     5281 agaagtttgg atgtaggtat gtagctacaa ctctgcgtag aagttattcg gcaagtagaa
     5341 atggttggag tgcaatgtta tacgaagcag aaaaagataa agcgtatttt tccacagaat
     5401 acaacataca aattgttgat cgcgttggtg gtggagacag cttttctgct ggtataatat
     5461 atgcgttatg tgaaaatatg tccggacaaa aggctattga atttgcgact gcatctagtt
     5521 gtttaaaaca tactatagaa ggtgatttta atcgaactac aattgaggat gtatataatt
     5581 taatgaatag cggtggaaat ggtcgcgtgt tgcgttagtc aacatataaa gataaatcac
     5641 agtaattttt atggtgatta tctgtcttag tagtgattag aataacgtga aaataaattg
     5701 ttgagcgttt atactgtttt gaaaaataaa tttaacatat tgaaaaatac aaatatatac
     5761 gtatattata aagcgccgat tgtaaaatta agctagacaa ctaaaacagt catttgtgct
     5821 acactcaaaa tagttccgac caaagaatta taaggagtga gtgcaaataa cctataccta
     5881 tcttacaacg gatgaacttg taataataga gtcttatttg aaaattaacc aatctgttgc
     5941 taaaactgcg cattgcttga atcgttcaag tcaaacgatc cataaagtat atctgttctt
     6001 caagcaaaga aaatcagcct tagaata
//
LOCUS       DAJSBC010000114         6002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSBC010000114 DAJSBC010000000
VERSION     DAJSBC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.116"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT65_002610"
     CDS             <1..395
                     /locus_tag="ONT65_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330194.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ONT65_002611"
     CDS             398..1228
                     /locus_tag="ONT65_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR2330195.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ONT65_002612"
     CDS             1244..2095
                     /locus_tag="ONT65_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2330196.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ONT65_002613"
     CDS             2092..2451
                     /locus_tag="ONT65_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCR2330197.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ONT65_002614"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ONT65_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ONT65_002615"
     CDS             2624..2929
                     /locus_tag="ONT65_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330198.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ONT65_002616"
     CDS             2929..3240
                     /locus_tag="ONT65_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR2330199.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ONT65_002617"
     CDS             3237..3410
                     /locus_tag="ONT65_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330200.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ONT65_002618"
     CDS             3407..3823
                     /locus_tag="ONT65_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2330201.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ONT65_002619"
     CDS             3823..4491
                     /locus_tag="ONT65_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2330202.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ONT65_002620"
     CDS             4488..4865
                     /locus_tag="ONT65_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330203.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ONT65_002621"
     CDS             4862..5161
                     /locus_tag="ONT65_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330204.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ONT65_002622"
     CDS             5151..5309
                     /locus_tag="ONT65_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330205.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ONT65_002623"
     CDS             5403..5819
                     /locus_tag="ONT65_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2330206.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAJSBC010000115         5793 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSBC010000115 DAJSBC010000000
VERSION     DAJSBC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.118"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..988)
                     /locus_tag="ONT65_002624"
     CDS             complement(<1..988)
                     /locus_tag="ONT65_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCR2330207.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="ONT65_002625"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="ONT65_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2330208.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ONT65_002626"
     CDS             1813..2967
                     /locus_tag="ONT65_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2330209.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ONT65_002627"
     CDS             3182..4150
                     /locus_tag="ONT65_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2330210.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ONT65_002628"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ONT65_002628"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2330211.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..5788
                     /locus_tag="ONT65_002629"
     CDS             5180..5788
                     /locus_tag="ONT65_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2330212.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1745 a         1131 c         1310 g         1607 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca cgggtgagct
     5581 tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg cggcaaatgg
     5641 aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg aaaacagtgg
     5701 gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac cataccccaa
     5761 tagctatttt gatttccccg ttaaataaac ttt
//
LOCUS       DAJSBC010000116         5790 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSBC010000116 DAJSBC010000000
VERSION     DAJSBC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.119"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="ONT65_002630"
     CDS             complement(99..1229)
                     /locus_tag="ONT65_002630"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR2330213.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ONT65_002631"
     CDS             complement(1877..3079)
                     /locus_tag="ONT65_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2330214.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ONT65_002632"
     CDS             complement(3139..3342)
                     /locus_tag="ONT65_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR2330215.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="ONT65_002633"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="ONT65_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="ONT65_002634"
     CDS             4879..5691
                     /locus_tag="ONT65_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330216.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAJSBC010000117         5764 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSBC010000117 DAJSBC010000000
VERSION     DAJSBC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.120"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..969)
                     /locus_tag="ONT65_002635"
     CDS             complement(<1..969)
                     /locus_tag="ONT65_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2330217.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKL"
     gene            complement(985..1101)
                     /locus_tag="ONT65_002636"
     CDS             complement(985..1101)
                     /locus_tag="ONT65_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2330218.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(1346..1423)
                     /locus_tag="ONT65_002637"
                     /pseudo
     CDS             complement(1346..1423)
                     /locus_tag="ONT65_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1577..2212)
                     /locus_tag="ONT65_002638"
     CDS             complement(1577..2212)
                     /locus_tag="ONT65_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2330219.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            2725..3867
                     /locus_tag="ONT65_002639"
     CDS             2725..3867
                     /locus_tag="ONT65_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2330220.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYVCDNPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYEY
                     GYYGYKEHYAYGEIKVMVSDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPTLSEKYKNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDNLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            3860..4066
                     /locus_tag="ONT65_002640"
     CDS             3860..4066
                     /locus_tag="ONT65_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2330221.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            4145..5338
                     /locus_tag="ONT65_002641"
     CDS             4145..5338
                     /locus_tag="ONT65_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2330222.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDRLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFQNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFINHKGKPKVAIDYTMLFVRMVKKYNKNHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
BASE COUNT         1900 a         1129 c         1055 g         1680 t
ORIGIN      
        1 taacttcaaa aactcatttt gcaaaataac aatttccccg gaataagcct tatcgatttt
       61 acataattca ccatttattg aagtatacat ttctgtaatt tttatttttt ccttttccga
      121 tattctaacc gaatctcgca aatgcagtac gccactataa agacgtatat atgcaagacg
      181 ctgtcttttt tccgaatact caattttgaa aacttttccg caaagttcag actgacctcg
      241 atgtgttgat gaataaaatt tattcgtaat cacttctata aggttatcaa tccctatatt
      301 gttttttgca cttccgtgat aaacagggaa cagggaacaa ttatgaaatc ttatgctttc
      361 ctcttgttcg agttctaatg cttccaataa tttcccagac gtatatttct ctaaaaggtc
      421 atcgtttccc tctattaccg tatcccattg ttcagattcg gtaaagttcg tcacacacat
      481 attaggatac agttctacct tctgtttgat tacaatttcg gcagaaagtt tctctttaat
      541 atcctgataa accgttgata aatcaattcc attttggtca atcttattga taaaaaagat
      601 tgtgggaatc cccattttcc taagtgcatg aaataatata cgagtttgtg cttgtacgcc
      661 atcttttgca gaaatcagta gaattgcccc atctaaaact gataatgaac gatatacttc
      721 tgctaagaaa tccatatgtc ctggcgtgtc tatgatgttc accttcgtat tttcccactg
      781 aaaagaggtt attcctgtct gaattgtaat tcctctctga cgttctaaaa gcgtattatc
      841 cgtcctcgtt gtacctttgt ccacgcttcc taattctgta atcgctccac tgttatataa
      901 taagctttct gttaaggtag tttttcccgc atcaacatga gctaaaactc caatattaat
      961 aattttcatg tgattttcct ccattcaaaa gcccaaaagg gcataaaaat cccagtgata
     1021 aatactctta tcactgggat ttttatgcat aaccataggc atacaaagca tacagatatt
     1081 ctccggatac tttagaatca catgataaag gtattcttaa actgggtaca aaaaactaag
     1141 ccctcctaaa aaaggacatc caattatttg ttcccactat caaattgaca gtttatttaa
     1201 gaataccttg ccgcatattt attaactcct tttaaataga tacttaaata atagcacgta
     1261 agagcatatt tgtaaaggaa tctccaattt tttatcaaag agagtacgtg attacaaaat
     1321 agctgtaata atgtaccaat atttgttatt ctataatctt ccaattactc ccgttctttt
     1381 caagtaccaa atcaaattga gatacctgcg ttgcctttgc ctgatttacc acaaatcaga
     1441 ttttatccct ttcttcctct tgattgccca caacagaaaa gaaaaagccc tgtcaagagc
     1501 tttgccacca ttggtggtgc tttgcactct tgatcgggct ttttgtttgc tgtatcttct
     1561 cgcaagagga aaaaagttac tcgtcctcat catcaaaatt gttattcctt aatacttctc
     1621 gtaacagttc catatcttct tttgagttcg ggtttatcat tttcacgact tgataaggtg
     1681 tcttatactt atgtccctct atgctttccc caaacatatc catactgtat aaatcatcat
     1741 agcggtctaa taatttttga aacttcaaat gctggataaa ttctttgatt gggtaaaggt
     1801 cggacgcttc aatgcttttc ccgttgatat agtctgtaat atattcccgt aacttttcta
     1861 actcatattc cagccattct tcctcgctgt caaagaagtt atcatagtgt ccatgtttca
     1921 gttctgcatt tagacgttct tcggctctta aaaactgaca gacttccttt tcagattgat
     1981 tgacatactt ccattctccc gacaacaatt cattgggcgt aacgtccagc acttccatta
     2041 tcttttccag cgtttcatag gcaggatagt tcaaccctct ttcaatcttg gaaaggctct
     2101 gcatattgat accgattttg tccgcaagtt cctgttgttt cattccccta aattttctta
     2161 tggtctgtat attatttcct aaatggctta ttttcattgc ataaccctcc atatctcgtt
     2221 atatcttcgc tattataagt ctatcatgct taatatgatt tgtaaaggca aaaaaagata
     2281 ttgacaaata agcatataag aattactatg ttcttgtaat cgaataagca caatagaatg
     2341 aaatataaaa acattcaaaa agaaatacct atctgattaa atccgtagat atgaatatcg
     2401 gttgaaatgg aacttgttag aaaaggcaaa acagaacttg ctgacaaagt atttctgaca
     2461 cggtggcgga agcctctcag gcttaccgcc atgacacctt gtgtcaaggg gaagaaatgc
     2521 tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc gttatctaac aggggggcac
     2581 tatttcacaa aagacaagtc aaaaaccata caaccccaaa gctgtacgga gtaatgagga
     2641 gttttaagaa gatagtacac ttcacacttt tttgaaaggc ggtttacagg tatttcaacg
     2701 ctattgtggg attggaggaa tacaatgaac aacttagaat ttgcacttga aatgaagaac
     2761 aaacgtcttg ccagcggtct ttcacaagga gaacttgcaa gtcttaccca tgtatcaaga
     2821 tatagtatca accgctttga gaacggaaaa gccaacgcca gcaaggaaac acaaaatatt
     2881 atcctgcgtt gtctgaatta ctatgtctgt gataaccctt tttatctgct ggtggattat
     2941 ctgtctgtcc gtttcccaac gactgacgca ttggaagtta tccgaaaggt gcttggcatg
     3001 aaagcagatt attttatcca ttatgaatat gggtattatg gctataagga gcattatgcc
     3061 tacggagaaa tcaaggttat ggtttctgat gatgaacaca tgggcgtatt tttggaatta
     3121 aaaggggcag gctcacgcaa catggaatac gtcttacagg cacagaacag ggattggtat
     3181 tcattcttaa accgctgtct tgactgtggc ggtgttatcc gacgttttga cttggcaatc
     3241 aacgatatgt gcggtctgct ggatattccc accttgtctg aaaaatacaa aaatggcggt
     3301 gcggaatgtc gctgtaaaaa ctatgaaaat gtgcagggtg gaaaactaag cggaaaaaac
     3361 cgtaatttag caagcactct ttatatcggc tcaaaggcaa gcacaaaata tttctgcctg
     3421 tatgaaaaac aaaaggaaca ggcaactaaa aagaaacata cggatattat caaccgtttt
     3481 gaaattcgtc tgcgtgacaa aaaggcagta caggcagttg aagaactgtt attgacgtat
     3541 aaccctcatg ggctggtgtt ttacctcatt accgattttg tggaatttcc cgactatcca
     3601 ttgtgggaga tattcatttc ccatgataat ttaccttttg aaatgaaccc tgtacctgtc
     3661 aatatggaac gcactctgca atggctggaa agacaggtca tgccgtccat tgtgatgata
     3721 gaagaaatcg acaggctgac aggttcaaac tatatgaaaa tgattgatga atgtacccac
     3781 ctttccgaaa aacaggaaat gcttgtggag cagatgtgta cggacatagc agacgtaatc
     3841 gaaagtgagg gggtgtttta tgagtaatgc acaggatatt cccgtatggg aaaaatacac
     3901 ccttaccatt gaagaagcgt caaagtattt ccgtatcgga gaaaacaagt taagacgctt
     3961 agcagaggaa aacaaggacg ctggctggct cattatgaat ggcaaccgca tacagattaa
     4021 acgccgacag tttgaaaagg tcattgataa attggacgca atctaatgca aatgagcctt
     4081 gtatgtgtta tgatgaacac aagtcatatc aaggctcttt ccaacaagga aaggagcaga
     4141 caccatgaaa gaaaaaagac gggatagcaa aggacgtatc ctgcatactg gagagagcca
     4201 acgaacagac ggaaaatact tatataaata tgtggacgca tttggaaaca caaaatatgt
     4261 gtatgcttgg agattgacac ccacagaccc gacaccaaaa ggaaaacggg aaaaaccctc
     4321 acttcgtgaa ctggaacagc agataagacg ggatattgag gacggtatcg acagcacagg
     4381 caagaaaatg acgctttgcc aactttatgc caaacagaac gcacagaggg caaacgtgaa
     4441 gaaaagcaca cagaaacaac gggaacagct catgcgatta ctaaaagagg acaggttagg
     4501 tgctaggagc attgatacga taaaaccctc tgacgctaag gaatgggcgt tacgcatgaa
     4561 agataaaggc ttttcctata acaccattaa caaccataaa cgctcgttaa aagcgtcgtt
     4621 ctatatcgcc atacaagacg attgtgtaag gaaaaaccct tttgatttca agttaagtga
     4681 agtcctagaa aatgatacca aagagaaagt cgcattgaca gaagaacagg aacaagccct
     4741 actctcattc atcaagacgg acaatgtgta tcacaagtat tatgatgatg tgctgatact
     4801 gttaaagaca ggacttcgta tctcggaact gtgcggactg acagtagccg atattgattt
     4861 ccaaaatgaa gttgtgatta tcgaccacca gttattaaaa agcaaggaac agggttatta
     4921 cattgaaacg cctaagacaa aaagcggaac aaggcaagtg ccattaagca gagaaacgat
     4981 acaggcattt caacgggtta tgaagaaacg cccaaaggca gaaccatttg tgatagacgg
     5041 acggggcaac tttctattta tcaatcataa aggcaagccc aaagttgcca ttgattacac
     5101 tatgcttttt gtccgtatgg taaaaaaata caataagaac cataaggaca acccattgcc
     5161 acatatcaca ccgcatacac tacgccatac gttctgcaca aggctggcaa gcaagaacat
     5221 gaacccgaaa gatttacagt atatcatggg acattcaaat atcagtatca caatgaactg
     5281 gtatgcccat gcgtccatag ataccgcaaa atcagaggtt cagcgtctaa tcgcataaga
     5341 agtatttacc acgattttaa ccacgattga cagggaaaat ataagaagat agacctagat
     5401 atgtgaggtt taccacaaaa gcaaaatgcc cgtagagccg ataaaataag gctttacgga
     5461 catttaagaa gatataaaaa gatagtcaaa aagacatata taatttatac ctaatttaag
     5521 taagtacaaa catcatttta agaataggaa aggacattta tatactattc tctgtatttt
     5581 tgatttataa ataaatatga attgaatcca aatcagctat attactattt tataataacc
     5641 aactataaaa aacgaagtta ggtgaaaaac taacttcgat attcataaaa gactctatta
     5701 gttcaactta gcaaaagatt taaacaaaaa aagatgaact atttctagtt catctttttg
     5761 cctt
//
LOCUS       DAJSBC010000118         5573 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSBC010000118 DAJSBC010000000
VERSION     DAJSBC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.121"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(59..775)
                     /locus_tag="ONT65_002642"
     CDS             complement(59..775)
                     /locus_tag="ONT65_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR2330223.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(1002..1544)
                     /locus_tag="ONT65_002643"
     CDS             complement(1002..1544)
                     /locus_tag="ONT65_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2330224.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(1549..1776)
                     /locus_tag="ONT65_002644"
     CDS             complement(1549..1776)
                     /locus_tag="ONT65_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2330225.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1855..3735)
                     /locus_tag="ONT65_002645"
     CDS             complement(1855..3735)
                     /locus_tag="ONT65_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2330226.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3924..4538)
                     /locus_tag="ONT65_002646"
     CDS             complement(3924..4538)
                     /locus_tag="ONT65_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2330227.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4680..5240
                     /locus_tag="ONT65_002647"
     CDS             4680..5240
                     /locus_tag="ONT65_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2330228.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         1628 a         1142 c          896 g         1907 t
ORIGIN      
        1 aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt
       61 aatttgcttg tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct
      121 cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg
      181 gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg
      241 catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt
      301 ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag
      361 ggactttaag accatataag tccacactca tttgattcgt ataacttttg tattgttgtc
      421 ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc
      481 acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag
      541 aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg
      601 tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag
      661 ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg
      721 tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa
      781 aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg
      841 atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga
      901 tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt
      961 tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc
     1021 tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct
     1081 ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca
     1141 gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt
     1201 tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa
     1261 cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag
     1321 attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct
     1381 tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta
     1441 aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct
     1501 gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt
     1561 tacttttgct gattggactt cataacctaa tttttccaca actgcagcta attgttcagg
     1621 gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt
     1681 attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca
     1741 agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt
     1801 gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga
     1861 tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat
     1921 caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa
     1981 gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa
     2041 aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa
     2101 gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc
     2161 agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc
     2221 ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac
     2281 ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag
     2341 catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg
     2401 tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat
     2461 gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga
     2521 aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc
     2581 agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc
     2641 aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt
     2701 ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt
     2761 ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat
     2821 tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt
     2881 accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag
     2941 aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat
     3001 aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga
     3061 tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc
     3121 gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc
     3181 atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata
     3241 ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac
     3301 gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac
     3361 aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt
     3421 caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc
     3481 cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga
     3541 gataactttg actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat
     3601 agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa
     3661 accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca
     3721 cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat
     3781 acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt
     3841 ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac
     3901 tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga
     3961 tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca
     4021 cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct
     4081 ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca
     4141 ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca
     4201 agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt
     4261 aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct
     4321 ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt
     4381 tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt
     4441 tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg
     4501 taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa
     4561 tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct
     4621 ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa
     4681 tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca
     4741 cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag
     4801 atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat
     4861 tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct
     4921 atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct
     4981 tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag
     5041 aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga
     5101 gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa
     5161 gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca
     5221 tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct
     5281 tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa
     5341 atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt
     5401 gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa
     5461 tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca
     5521 tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa tca
//
LOCUS       DAJSBC010000119         5306 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSBC010000119 DAJSBC010000000
VERSION     DAJSBC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.122"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..265)
                     /locus_tag="ONT65_002648"
     CDS             complement(<1..265)
                     /locus_tag="ONT65_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330229.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSI"
     gene            complement(563..1537)
                     /gene="sgrA"
                     /locus_tag="ONT65_002649"
     CDS             complement(563..1537)
                     /gene="sgrA"
                     /locus_tag="ONT65_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR2330230.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1733..2572)
                     /locus_tag="ONT65_002650"
     CDS             complement(1733..2572)
                     /locus_tag="ONT65_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2330231.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2757..3644
                     /locus_tag="ONT65_002651"
     CDS             2757..3644
                     /locus_tag="ONT65_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2330232.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4228..4923)
                     /locus_tag="ONT65_002652"
     CDS             complement(4228..4923)
                     /locus_tag="ONT65_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR2330233.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4907..5305)
                     /locus_tag="ONT65_002653"
     CDS             complement(4907..5305)
                     /locus_tag="ONT65_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2330234.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1690 a         1072 c          780 g         1764 t
ORIGIN      
        1 gtatagaata aactttactc agtaaaaact gagaagaaaa ctcgactttt atcatcttgt
       61 tttctgaaag aaaagataat ttatattctg gatacctttc gcaaaacgat tctgcttgtt
      121 gaatcaccga attcatctta gccagggtgt agtttagatc taaagcgata tccttcacca
      181 catataattt ctcgtctctt tcataacaat agttcaaaaa ttgatatact tgcctttcat
      241 tgatatcaat aaattcttca aacaaagtac atactccttt ttatctatta tttagactta
      301 ttccgacagc tacatgaata acgtacaaat agattcgtta ctgatttttc agagatacta
      361 ccatgcgcag tgtgcattgt caattattct tgctcttatt ttccatttgt gttcctgtta
      421 tttcctgttt ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct acaacttcgc
      481 caggcaatac agcactctcg aatgaaatat tcactcaaaa atctatcgtt ttattcaatt
      541 ttagatctgt tgcttttatt ctttatttca ctcgatggaa gagagaacga aaatagcttg
      601 ctcctacaat caaaatgagt aacacaccca tccatgtaat tagcttgttc aaggttctac
      661 taccagtttc tggtaaatcg ctttttctat tatctgtttc tttcgctttt tcttgatgat
      721 caattccacg agaaacaaaa tcaggctgtt ttagcacact ttttaggttg ctccctgcat
      781 ttctcttttg ttccttagtt ggtatgatag gtaccaaatc aagttcatta tttacttttc
      841 ctgtgctgct ttcgctgctc gtgcttcctg tgctactctc actactcatg cttcctgtgc
      901 tactttcact actcgtactc tccgtactgc tttcgctgct cgtgcttcct gtgctacttt
      961 cactactcgt actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc
     1021 tcgtactctc tgtactgctt tcaccgctcg tgttttctgt actgctttca ctacttgtgc
     1081 tatccgtact gcttggcggt ccaacaactg tccaactgct tggtttcaaa ttctccgtga
     1141 tcgtcatctg tccatcataa aaaacaatca aatcaacttt atctttactc ttgatctcat
     1201 caatcgatag ttctgcagaa atcattattg tatactcaat accagcggtc cattgtacgt
     1261 cttttaaata aacttcccca ataacttttt ctgaacttcc attaccatcc caaccatatg
     1321 ttagaacagc atgtacatca ctagcattca cgcttttgtt tggtttgact ggaatagtaa
     1381 tgacttgttg tgtcatgtca taaacaatcc gatcagtttt cacagtaaat gaaagattct
     1441 catttgcccg ttcatttgca gaagtactga ttccaatcat tgctgcaatt atactcagcc
     1501 caagaataac aaataatacg gtcgctgttt tcttcatttc tatcactctc catataatta
     1561 ccttctattt gataaaggaa ttatataaaa tgaaaatgat gagaacacga cagtcagttg
     1621 tgaaaatcta acaaaagatg tcgtgtattc gcttgaattt gtttactttt tcgtttaatc
     1681 aatgacagac ttaaaaaagc aagaccgata tcagccttgc tttcttcatc ttttattgat
     1741 aaacagcagt gtatttcttc atccgctttg ttttatatgc atagagtaac aaaccaacaa
     1801 tcaagactaa caagaataat gtagttgata tagatgcttc agccccaaag tttcccccag
     1861 taattattgt attttgcgat tggatcagag tggagaaaaa accttgttct tgcgttccgc
     1921 tgactggtat acccaaaata gctccttgtg tccaattcca agtaatatga gcaccaatag
     1981 ccgtaaataa acttcctgta ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga
     2041 tattgaaaac tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg
     2101 cagcagatcc aatcattgct attaacggac caaatctaag catctgatgc tgaaaaaagc
     2161 cacggaataa aagctcttct gatgccccca ctgcaataaa taaaataaaa ctcgttatca
     2221 gcggcacaat gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa tataatggac
     2281 tagtaataag cagaaacact ccaattccaa caacgagacc tcccgctaaa tacccccagt
     2341 cttttcctcg aacagaaaca ccataatctt ctattctttt tccttcgacc ttactagcaa
     2401 gaaataaagc tagtaaaaaa acagctaaat gtccgaccgt accggcaatt gctccaatcc
     2461 attcactatt tccaataaac atatccgcaa gtgcattatt gacaagaatt cctaaaacaa
     2521 aaccaactaa gactattaaa acagatccaa tcaaccgaat aatcctgatc atctttcatc
     2581 cctcctttat attaagttta acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat
     2641 gttctccatc atagagagaa tagattttta cgaactaaga atatctacag caaccttacg
     2701 aaaatgaaag actgtctccc taattcttct gttagactgt ttttaataag gaaaggatgg
     2761 gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca attttactat
     2821 tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga acacttacag
     2881 ttcaggattt ttatgataac ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa
     2941 tgattgtctt caaagcattt gatgatgtat acggaaaaga gatttcttca aaggaagtaa
     3001 caaaagaata taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag
     3061 cagttggcca aacagaagag acatataaga aactctttaa acagatgctg gctttccaat
     3121 acggtttgaa agccaatgta aaattaacag ataaagatct agacactgct tggaaagaat
     3181 tctatccaga agttagtaca cagatcattc tattttcgac taaagaagaa gcagataaag
     3241 caaaaaaaga agcaaatgaa ggggaaaatt tctccaagct tgttcaagct tatggaaaaa
     3301 ataaaacatt aaaggaaact gatggaaaaa tgaattttga ttcaaccaat ccagagattc
     3361 ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct gatatcattc
     3421 ctgtaacaga tccaacgact tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac
     3481 aggataaagg ttctaataaa gacaaataca aatctgagct agaaaaaatt gctaccgaag
     3541 caaaaactct tgatacggaa ttcatggata aaacaatccg taaagtgatg aaaaaagaca
     3601 atgtaactat taaagatcct tatgtaaaaa atatattcaa ataaacaaat ttgacccaat
     3661 tggggatgtg gcaaaatttg agaagtgatt tttggaaatt ccttcttatt tttgaggcaa
     3721 accacttttg ccacaacttc atcggggcct ttttttattt gtaccaaaac aaatcactgg
     3781 atttgttaca atcatgagga tataatttaa aaaagttttt tatttctatc acatgaatca
     3841 atgcgccatt aagtagattc tttcatgctt ccctattttt tcacttgccg tatcgcttca
     3901 aaaacaacta tcataaactc aaactcgact gaaaagttat tttaaaaaag actaaaaata
     3961 tcccttctgg acttgttcct cttattctgt tatactagag aaacacgaaa gatattttta
     4021 tgcgacactt ttcatttgtt tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta
     4081 caaggatgta ttagaggtta gggaatacat cgtttaaatg agaaataagt aatagtcacc
     4141 caaactttaa attaatgaat attgaatcgt cagatagcct cctcgagttt tcgagaaggc
     4201 tatcgtttat tttatggaga aattgattca gaagtaatca gcgacctttt ttagttcacc
     4261 aatcgtttct gcttggaaag tttgcaccca atcttcttgg aagcccctca ctgctttatc
     4321 aatcgagggc atctctaata cttgatctag tagctcttta cctaacgtta tcgcatgagc
     4381 tccagatcga atggctgtag tgacttgttc acaatttttg aaactagctg ctaatatttt
     4441 tgttggtctt tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc
     4501 aatgccttga ttttgcatcc tgttataata aggtgcaata taatctgctc cattattaat
     4561 agctaaatac ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt
     4621 tttcaacttt ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga taaacgtatc
     4681 ttttcccagt tcttctatga taactcgagc atcctctatc atttcttctt ccgttcttcc
     4741 tattgtctga atatggatag gaatctgtcc gattattttt ttgatttctt tcatatgcga
     4801 gaagaagttt gacgctcctt cttttttcag aattgtagga tttgttgtta cgcctgttaa
     4861 attcaaaaac tgttgacaag aataaatact ttttgtattt gctgtatcaa gcatgatttc
     4921 catatagctt tcccccattc tgatcctctt gatcgatttt aatgattgtc ccctttctta
     4981 aatgcggcat ttccttatct tctacataca ttgttcctgg aagttccggc tcatgagatc
     5041 catcaaattt tatcgtgata tgtcctaatt cattcaaatt agtttggaca acatctccta
     5101 ctgctgtgat tacatacatt tgtttatcaa aacagagctt catatcacaa gttatttctt
     5161 ttcttacctg attcaattca atcttataac aataatctgt taatgtttcc ggtgcttcat
     5221 ctccaaataa aatcattatt ttttctttcc tgaatacttc tgcttctata ccaacatctg
     5281 ttacaaccgt ttcaaagatc gtcatc
//
LOCUS       DAJSBC010000120         5250 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSBC010000120 DAJSBC010000000
VERSION     DAJSBC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.123"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1666)
                     /locus_tag="ONT65_002654"
     CDS             complement(<1..1666)
                     /locus_tag="ONT65_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2330235.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1708..2766)
                     /locus_tag="ONT65_002655"
     CDS             complement(1708..2766)
                     /locus_tag="ONT65_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCR2330236.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2885..3340)
                     /locus_tag="ONT65_002656"
     CDS             complement(2885..3340)
                     /locus_tag="ONT65_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330237.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3924..4727)
                     /locus_tag="ONT65_002657"
     CDS             complement(3924..4727)
                     /locus_tag="ONT65_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2330238.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1551 a         1026 c          727 g         1946 t
ORIGIN      
        1 aattttgacc atatactctt tctttcctag gtggaagacg taaatctata tttaaatgtt
       61 ttcgtactaa ttcagcactc ttcaacattt cattataagc aatttccaga agagtttgtt
      121 cggataaagc ccaaaatgga actattcgtc caaatcggtt aataacttca ggctttagac
      181 cttcaccaat caaggcttgt ttgatccctg atgcgacttt tcggtattct ctttttagtt
      241 cagtgctgta ttcattagaa ttttccttgt ttgtaacacg agatagtgag ctatatcggt
      301 ttaacgaatc gaacatctcc gcacctgcat ttgttgtcgc aaataaaatt gtatttgaca
      361 atgaaacggt gtcgtgaact tctcgatcat cccatcctct ttcagtaaaa cttatcctac
      421 cttcatctaa aattgataaa aaggcatcga gtgtttctac accagctttc tcatattcat
      481 cacacacaat aatcgcagaa ggatcatgcc ttacatgagt acctataagt cgtctaaaac
      541 gcattgcatc atctttggtt ttgtagtccg gcatattaac taagagaact ttattttcat
      601 cgccataaag tcctcttgcc acggctttag ccattgcagt tttaccgacc cctgttgatc
      661 ctgtaaacag agcagttgct tttggttgct ttcgaccttt aaaacgatca aatccaagct
      721 tcatacgttt aaacaacatt gttatagtat aaactgctaa cggttgtcct tttatggact
      781 ttaattcctc ttgaagtttt tccatatcaa aatcagaatc tagctgaata ttatacttac
      841 caaatatttg caatagactt tctcgattat aagcatttcc gtctactcga tcttttgcaa
      901 tcaaatcctc cattacagca attgatttag ccggttcgga caaatcctca cgataaagtt
      961 tgtttgcttg tacgatttct tgaagtaatt catcaggaat cctttgcgat aatctaaaag
     1021 aagcctgagg atcatcttta acagaggatg tatttagcca ttgtctcaaa atatcgacga
     1081 caacatcgct ttttagttca ttcaattcta acggttgtag ccgtctccct aaggcatcat
     1141 ctcttttgat atagttttca tattcttttc ttgtagtagc ggtaatcatt tgaagtgatt
     1201 catttgctaa cggttctttt agcgaatcac ccccaatttt attgccactc ccaaaagagc
     1261 taacaacttt atgaaactcg tcaatgaaga ggacaaattc attatctttg tcctcagctt
     1321 ttaaaattcg ctcatattct ttcaatcttg gaattaatgt ttctaaacgt gtttggagga
     1381 catcatctcc cccaacacgc aattgtccga ttgataattt atatacttgt actttctttc
     1441 catcttgtcg ctgtctttca gcccattcac ttgcaagtgc tgttttacct gttccggctt
     1501 cacctaacaa gatagcttgc ggtgtttccg gtctattcaa gatcagtgac aactcatgga
     1561 gttcgtcatc acgtcccact aacttatgtt ttggtggttt cattggtgtg agagcatgat
     1621 taaattcggt ttgaatattc ttaaaaaact cggtactatt ttccatctgt ttgtgttcac
     1681 ttcctctaaa ataagaataa tttacttcta gtcagtcaaa gttaggtact taaatgtcgt
     1741 cttcatgtct ccaaaagaat ctttgttaaa gtcagcattt ttgtattgat caatcaattt
     1801 ttgtgtatca ttgattgctt taatttcggt ctccattcga ctgtctagtt gacgaatact
     1861 ttcttgcgaa tctttatcgt caggattttg ttgtaaacgt tccagatact cagcttttgt
     1921 ggcttcaatt ttcttgatgt tagtatttga agcatttaac tgatcttcta gcttatttac
     1981 aacattattc ttacttgtaa ctgctacaac ttttgaataa tcaatatcgc catctttggt
     2041 aagaaagctt ccttgataga cattagttga atctgaatat ggatttattc tgaaattgat
     2101 attatcagga gtattaacag gttttgcttt tttcccaaaa gaaattactc cttcattatt
     2161 aacatcattc atagaatatt gagaaagtgc ttttgtaact gaatctgctt cattcacatt
     2221 gactttggta tcatcgtcag ctgtatcatc accagaaatt agtgatactt ggtttgcaat
     2281 aaaaacttga taagctcttt cctctaattt accttttagg aataagaaag catccccatc
     2341 agtacttaac atcccatagc tcatttcaag cttcttagga cgatcttttt cttttgttgc
     2401 catatataag ttatagtttt ttccttgcgt agataacaac tttctagcgt tttgggagta
     2461 accaagtttt actactgtta catctctatt ttgatctgtc cacacatctg aaatagtgat
     2521 ttctgctcct gatcgagcaa actccaatga agaccctata ggtgttgtat tgttcacgag
     2581 ataataatta tagtttccta attgataaat gtagctaccc aagcctgtta ataaaaaaag
     2641 cactcctaga aaagttgcaa gcataatgcg taatccgtga cgtgttctct tatccttatc
     2701 accaagcaac cctccaataa tactgtattg atctttggta gatgttgaat tgacgttttt
     2761 ttccatcagt ttgtctgccc cctttcataa aagagcaaac aatcagacgt tttttcggta
     2821 attctaggtt cgggattttg aatcatttcg gtatattctt ccccattaat atttaacaca
     2881 aaaattatct tcctttcttc gttcctttct tttgctcttt attttccaaa taatcaatga
     2941 tttttgctat tgtaccaaga tagtaattcc cctcagcttt gttttttcgg attttgctaa
     3001 ccattggagt aggtacaatc tcttcatctc tcaattcact gaaagatttt ttacctagat
     3061 attttccaat aacagatttt aaaaaacgtt caaaatcttt ctcgtaagtt gttttcttgc
     3121 cttttagttc atggttcata ttgtcaacga cttttgcggt ttcaataatt gttttagttc
     3181 tcagcttaat aatttcatct ggaattttca atcctcgtaa tttcatcgtt gtactaactg
     3241 gtaacccatg ctttactgca tcagcaatac taaaactttt tagatcatct agtaagctct
     3301 ccatatagtt aacaaattca tgccaattaa tagcattcat tgtctcaccc cttcattttt
     3361 ttaaaatgag gttcgggcgt aacacattta tgttattata atatcataat aacataaata
     3421 aatcaatcag caaatttaga tatcccttaa agtatcagca attattcaac aagatattta
     3481 ttttagctat ttcagtcatc ataaaagact aattagcaat aaaataacta atattatcca
     3541 tttatcagac gaaaagaaaa aacttatcta attactctat attaactttt aacaaatagt
     3601 tgacttgtcc aaaaatgtta ccttcaatct cacaaagata ttaagatgtt tcctaccaaa
     3661 actgatatca ccggactttt tattctgtgt tgtccaaaag tgttaccttt agtcgatcaa
     3721 caaattaaaa aaaattgttt ttttcttgac ttgtccaaaa gtgttaccca tagttgttaa
     3781 aatttgtata gattagccat tctttcgaat attatttaga ctgaaacttg cttacaaaaa
     3841 aacttcttgc atttattgtc caaaagtgtt acccatactt ttttaaagaa gcaggttgta
     3901 ttaaaattac aacctgcttc ttctcaatct tcttctaacc aattatgcat tgaaatggtt
     3961 ggcaagtttg attctggctc actaaaagtg aatctgagcc gatcaactct tcttcctttc
     4021 tttgcataga ttttttcata atcaaaagtt tcaaatacct gtattccgtt ttcatcagga
     4081 gctaaaaatt cttctttcgc ctgctctagt attcttttgt caatatttgt cattctataa
     4141 cttgaaggta tacttaatgc tcttctaaaa tcttcaactt tgactaccca ataaccattt
     4201 cgccactctt tatttctgta ttgcatgaga tgacgataca attcttttgt gtagctactt
     4261 cttagttttg taaaattatc aagttcaaaa cgagtaaagt ttttgccaat tgagtttaag
     4321 ataaattcaa attcctcatt tgccttaatt gtaactgttt ccttctgttt atctatccgg
     4381 aatttttgaa aaagccacag ctgttcatag acgtctgaat cttcaaaacg gtaggttaaa
     4441 gtacctagtt tgtcactcaa agcttttaac acagaataaa aactgtcctt ttctcttctg
     4501 tcgtatttga ccaaataacg aaagaggttg aaatcaaaag ttacttctgc tgttccttga
     4561 cgtttcattt ttgataaaat agcccaaaac acatccattt caacaggctt aaatcctcgc
     4621 aaagctgttg tattcagacc attgttataa cgcacaattg catttgtttg ttctgatact
     4681 aaatctacat cactatcaat aatcttttcg gtaacttctt gagacattga ttactctcca
     4741 atcttctttt tgcgctcctt taatcatgtt aatctggtac taattaaagg aaacaatttt
     4801 ttctgcttgt tacctaaatt aaatcacaac taaaggtaac agtcaacaaa gatttgttac
     4861 taatctaatt ttctattttt tcgccttcca aatcaagaaa accgaaggta acgtcttatc
     4921 ctagtgtgat acctcaaatg tccacttttg ttacctttgc tgtccaaaag tgttaccctt
     4981 actgtccact tttgttacct ttactgtcca aaagtgttac ctttataccc ctttaatact
     5041 tactctccca agagattaag cctcccctaa cagatatatt aacagatatt taacagatat
     5101 attaacagat atttaataaa ggaggattac tcctctctta tttcaaaatc ttaaaaataa
     5161 aaaatcttaa aaaaaactaa cactatctag gagtttattt tgcttgtgta actttgaata
     5221 attgaaaaaa aggaaaagga ggaagcttca
//
LOCUS       DAJSBC010000121         5212 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSBC010000121 DAJSBC010000000
VERSION     DAJSBC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.124"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            604..954
                     /locus_tag="ONT65_002658"
                     /pseudo
     CDS             604..954
                     /locus_tag="ONT65_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="ONT65_002659"
     CDS             948..1370
                     /locus_tag="ONT65_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330239.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="ONT65_002660"
     CDS             2540..3508
                     /locus_tag="ONT65_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2330240.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="ONT65_002661"
     CDS             3525..4049
                     /locus_tag="ONT65_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2330241.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="ONT65_002662"
     CDS             4425..4706
                     /locus_tag="ONT65_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330242.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="ONT65_002663"
     CDS             4708..5124
                     /locus_tag="ONT65_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330243.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          893 c          868 g         1484 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAJSBC010000122         5199 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSBC010000122 DAJSBC010000000
VERSION     DAJSBC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.125"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..696
                     /locus_tag="ONT65_002664"
     CDS             25..696
                     /locus_tag="ONT65_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2330244.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ONT65_002665"
     CDS             755..1195
                     /locus_tag="ONT65_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330245.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ONT65_002666"
     CDS             1207..1875
                     /locus_tag="ONT65_002666"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2330246.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ONT65_002667"
     CDS             1939..2562
                     /locus_tag="ONT65_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR2330247.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ONT65_002668"
     CDS             2618..3004
                     /locus_tag="ONT65_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR2330248.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ONT65_002669"
     CDS             3217..4608
                     /locus_tag="ONT65_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR2330249.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1728 a          861 c         1095 g         1515 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAJSBC010000123         5196 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSBC010000123 DAJSBC010000000
VERSION     DAJSBC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.126"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            235..1376
                     /locus_tag="ONT65_002670"
                     /pseudo
     CDS             235..1376
                     /locus_tag="ONT65_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1502..2581)
                     /locus_tag="ONT65_002671"
     CDS             complement(1502..2581)
                     /locus_tag="ONT65_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2330250.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2684..3124)
                     /locus_tag="ONT65_002672"
     CDS             complement(2684..3124)
                     /locus_tag="ONT65_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2330251.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3181..3591)
                     /locus_tag="ONT65_002673"
     CDS             complement(3181..3591)
                     /locus_tag="ONT65_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2330252.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3771..4205)
                     /locus_tag="ONT65_002674"
     CDS             complement(3771..4205)
                     /locus_tag="ONT65_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2330253.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4305..5153)
                     /locus_tag="ONT65_002675"
     CDS             complement(4305..5153)
                     /locus_tag="ONT65_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2330254.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1890 a          987 c          867 g         1452 t
ORIGIN      
        1 atgttcagat aggaaaagaa aaatatctaa ttttgtattg ctttttttct tcaagataaa
       61 cgttttaatc gcttcttgag tttgcatgaa agaggctcct ttttacttaa attgttaaaa
      121 cttgaaaata ttactgtttt tttcataaat ttagacaaaa aaacagtaaa catataggta
      181 gaatctctac tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga
      241 tattcaacca tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag
      301 acagtccaca cagaggagac agaaacagcc aaatggacag aaaccaagtg ggccaacgtc
      361 aatcgaatta cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga
      421 aaaggacaaa cagcgaccat caaagcaaaa attgactatt cgggagactt agcacctata
      481 ctacaatcag ctgttacatt tgagaatgtt gatcccactt tgtcgattgg ctatgacgag
      541 aacactttga ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag
      601 gacttgttaa aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat
      661 catttaacgc cgtatagtca gcggcagaca attgagcaag attcagccat cgactcagga
      721 ggagatcttg ttgaagaacc aacagataaa ccagaaaatg aaaataagcc agaagtaccg
      781 cctacagaaa atccagacgg agaacagaaa ccggaaatag aacctggaga agaacccgat
      841 acagaaactc aaccagagcc agacaataaa tcaaaacctg aaataacccc tggagaaaaa
      901 ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat
      961 ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa
     1021 ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata
     1081 aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt
     1141 ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca
     1201 gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg aaaaataaac
     1261 agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat
     1321 taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt
     1381 tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa atgaccccaa
     1441 aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat
     1501 tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat
     1561 ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt
     1621 cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca
     1681 acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat
     1741 aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa
     1801 tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca
     1861 gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt aaaagagcga
     1921 caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt
     1981 aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg
     2041 tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg
     2101 ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt
     2161 caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt
     2221 ttggctgatg ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt tgatgaataa
     2281 gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca
     2341 tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa
     2401 tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga
     2461 aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa
     2521 aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca
     2581 ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa atgacgttat
     2641 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
     2701 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
     2761 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
     2821 cagcaccttg agcagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
     2881 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
     2941 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
     3001 tcacaagata ctgggtatag gtaagattca gcggcttgag gaggggagtg tagtgtctaa
     3061 tgatttcttt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
     3121 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
     3181 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
     3241 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
     3301 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
     3361 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
     3421 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
     3481 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgcacttc
     3541 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaagttacc
     3601 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
     3661 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
     3721 caaacatata caaaaaatat cagagacaaa aaataatctc atcataaaca ttacatatat
     3781 ttctcaaaaa gttgttttct ttcttcaaaa attacttcac gatggacctg atgaagcatc
     3841 cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc catcatttct
     3901 gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt gtcctcgata
     3961 tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg agcagtcaaa
     4021 ccaagagacg tgttttcatc cagcatatgg aatctcgtac catacaaggt aaaaatgact
     4081 tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc aaagattgtt
     4141 tgatacagtt ctagtgcttt caaactatca tttaatacaa aatcgatttc cgttccttta
     4201 tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt attataccag
     4261 ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc gattggatga
     4321 taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa ggcacttgga
     4381 tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac aacgatcaat
     4441 aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt aaggccacgt
     4501 ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact catgaccata
     4561 acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga agtgattgca
     4621 atagataaaa tagcataaag aaactcttct attccaaaaa taaacagatt aagagaaaca
     4681 acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata ttttttaaaa
     4741 atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa aatcgcaact
     4801 cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga agtaatcatt
     4861 gtttctggaa ctattgcaat gattaaagga aaaaccaagc ttccgaaaag tactttaaaa
     4921 aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt caacactaaa
     4981 ataactatag ctcggtcaaa gcctaaagca gcttccagta atattcctag accgctgaca
     5041 cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc taatatgaag
     5101 agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt catttattca
     5161 cttccttaat tcattaataa tactcttatc ttatta
//
LOCUS       DAJSBC010000124         4890 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSBC010000124 DAJSBC010000000
VERSION     DAJSBC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.127"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..258)
                     /locus_tag="ONT65_002676"
     CDS             complement(<1..258)
                     /locus_tag="ONT65_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330255.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ONT65_002677"
     CDS             complement(274..924)
                     /locus_tag="ONT65_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330256.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ONT65_002678"
     CDS             complement(935..2107)
                     /locus_tag="ONT65_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR2330257.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ONT65_002679"
     CDS             complement(2126..2998)
                     /locus_tag="ONT65_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2330258.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ONT65_002680"
     CDS             complement(3020..3940)
                     /locus_tag="ONT65_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330259.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ONT65_002681"
     CDS             complement(3940..4209)
                     /locus_tag="ONT65_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330260.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ONT65_002682"
     CDS             complement(4206..4586)
                     /locus_tag="ONT65_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR2330261.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1296 a         1064 c          801 g         1729 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc
//
LOCUS       DAJSBC010000125         4746 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSBC010000125 DAJSBC010000000
VERSION     DAJSBC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.128"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            7..1197
                     /locus_tag="ONT65_002683"
     CDS             7..1197
                     /locus_tag="ONT65_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCR2330262.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1331..3520
                     /locus_tag="ONT65_002684"
     CDS             1331..3520
                     /locus_tag="ONT65_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2330263.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3517..>4746
                     /locus_tag="ONT65_002685"
     CDS             3517..>4746
                     /locus_tag="ONT65_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2330264.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1703 a          727 c          856 g         1460 t
ORIGIN      
        1 ttccctatga acgattttac tacagaaatt ctaaagactc tagcgaacaa aggcgatttg
       61 aatgaattat tccgtgtcca tttggagaaa gcagtcaata cgcttctcaa aacggagtta
      121 acggctttcc tagattacga aaagtatgat cgcattggtt ttaacacggg taattctcgt
      181 aacggctcct atgaccgtac ggtcaagacc gagtatgggg aacttcatct ccagattccg
      241 cgcgaccgca atggtgagtt caagcaacag actgttcctg cttatagacg gacgaatgac
      301 acgttagagg agaccgtcat tcacctcttc cgaaaaggta ttaccatgtc ggaaatcgca
      361 gacttgattg agaaaatgta tgggcattac tacacgcccc aaaccatgtc caatataaca
      421 aaatcattta cagaagaggt aacggcgttt aatgggcggg agcttcatga ccgttatgct
      481 gctatttata tggacgcaac gtatattccg ttaaagcgga aaaccgtcgc caaggaagct
      541 attcatatcg cagttggcat tcgcccggac ggatcaaagg aagtattgag ctatgcgatt
      601 gcaccgactg aatccatcac gatttgggag gaaattttat tggaccttca agagcgcggt
      661 ttgaaaaatg tcctcctgtt catcacggat ggcttaaagg ggatggtagg agcgattagt
      721 cggttctatc ccaaagctcg ttttcaacat tgttgtgtac acgtttcccg taatatcagt
      781 cataaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg attttaaaat ggtgtatcaa
      841 gcctcatcta aagaggtggc gttggaagca cgtggtactt ttgcggaaaa atggaaaacc
      901 agctatccaa aagtggttga atcgattctt tcgaacgatc acttgctcac tttctatgat
      961 ttccccttgg ccatacgcaa gagtatttat tctacgaact tgattgaatc ctttaataaa
     1021 caaatcaaga aatacagcca ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt
     1081 ttcttagtct cgtcttttga tacttacaac caaaaattcc taggtcgcag tcataaaggt
     1141 ttccaacagg ccgaaggcga gctcgaacaa atgctaagcc aactgattga gaattagaaa
     1201 caatctgcag tagggaagaa tcatttacac aaaattattg acgctccctt ataatgagaa
     1261 cttttactaa cataatttat gataaactta ctatataatc atattaatta aatagttagg
     1321 agatctgctt atgtttatat ctactcttca aattcgaaat tttagaaatt atgaatccga
     1381 aatctttcga ttcgataatg agacaaatac tattataggt gagaatgatt cagggaaaac
     1441 caatgctctt actgcattga gaatcttatt agatgatagt tattactata gtagtaaaac
     1501 actaaaagaa tcagattttt ttcatggaat tcaaaatggg tggcaagggc attggattat
     1561 tatctctgcg acatttgaag gaatcagcga agaagaattt gacaatgaga tttgtgcttc
     1621 catttcttta aattctgaaa gtcaaacaat cttagaagaa ttgatttcaa atgcagataa
     1681 aggtgtaggt agtatttctc tatttattag acctaacaaa gctattagaa agcaattatt
     1741 tgatatttcc actgaattag actctcatca atttaacgaa tttagaaatt ctattcgtct
     1801 atccgactat gaattttact acacatctaa atcaaatcta aatttctgta tcaatgaaaa
     1861 ttatgataaa ttggtaggaa gactcaatga caatatagct agtaatcctg aagaagacga
     1921 tgaagctctg ttgggctcaa gaattgacat gtcagatatt ttcaagcata tttcggttgt
     1981 ttatgttgac gctcttcgag atgttcttag agaaatgaaa aacaatcgaa acccagtcaa
     2041 aagaatcatg gaaacaattg aatcaaaaat atcatcagat aatgttgagt cattaaaaac
     2101 aattatacag caattaaatg agactattac cagtgttcct gaaattagga aaattggcga
     2161 aaatattaat cggcaactaa atagcataat tggttcagtg tattctccta atcttcttct
     2221 ttcctcaact atgtcagatg atatgggttc tttagcaaaa tttctttcaa tgaagccaga
     2281 acaaaatatc gacttagact tactagggtt aggacatcta aatatgattt atttagcttt
     2341 gaaaatagtc gaatttgaag cgtgtcgttc aagagaacta cttaatatta tgttgattga
     2401 agaaccagaa gctcatattc atcatcatat tcaaaaaact ctgttcgaag gattaaatct
     2461 tcaaaaaaac tatactcaga ttctcatgac cacacattct gtacatttag ctgaagtgtc
     2521 tgaaatatct cggatgaatg tattaaaatc gtttaatggc aaatctatcg ttatgaatcc
     2581 agcaaataat ctgaactcat ttgctaaaga aaagttaaaa aagaacaatc taaatctaat
     2641 aaacgcagta gagagatatt tagatgttaa aagaaatgga ttgctttttt ctaaaggtgt
     2701 tatcttagtt gaaggagatg ctgaagaaat acttattcct caaatagtaa aaaaattatt
     2761 tggtataaac ttagacgaac ttggaatagg tttgattaat gttggtagta cttcgtttga
     2821 atatatcgct agcttgtttg acgatgatag aatacagaga aagtgtgcta ttgtaactga
     2881 tttagatgaa caggcaatcc catcagaaca tcgattgtat aaaagcactg cacaagagaa
     2941 aggtcaagca agaaaagaaa aattatcctc cctttttaat gaaaacaatt gggttaattg
     3001 tttttatgct aatacaacat ttgaaattga gtttttagat gtgaataaag aaaatattct
     3061 aacttatgtc agtccaatta tagatgacgc attcaaagat aaaaaaacaa taactgctta
     3121 taaagaagaa atacaaagtg aacaatattt agaaagaaat ttagcgatgt taaaactagc
     3181 caatcatgtt ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg actttttaat
     3241 caaaattcct aagtatattc ttaatgctat tgcttttgca tgtcaggaaa tagtagatct
     3301 ctcaatttat aaaaaaattc tactccatac aatcgacttg tacgaggaaa ctacagaatt
     3361 gtcagcttta tcagctagtt taaaaacaag tgatacagct gatttgaata tagcaataat
     3421 ttctttatta gagtactttg gtaaagatga ggatgttgtg tatctaatca atcagataga
     3481 aaattacaca aaaatacttt atgctgaggt ttcaaaatga caaatatctt cgaaaaaatc
     3541 gtaaatgatt taaatttaga acaaaaagat gttgtggaaa gtgaagataa cctttatgta
     3601 aatgcatgtc ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc ttaccaagca
     3661 attaaacacg atggatcact aaaaagaata attgctatca catacactaa tagagctgct
     3721 gaagaaatta aggaacgcct aagtttactt agtatagatg atgatattaa tatttgggtg
     3781 gggacaatac atcagttttg tcttgaattt attatttatc cttttaaaat gaatctctca
     3841 agagtatcaa aaggatttac aattattgat gattatactc aaagagctta cgtcagccaa
     3901 attaataata tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat tgatttgtca
     3961 ctttctcatg attttaaaat taatgaagtt cagtatactc aagtttctga aatgtatcac
     4021 actatgttgg aaaaaaataa agaaattgat tttgatatga ttttactagt agcgtataaa
     4081 attttaactg taaacagtaa cgttgcacgt aatattgcta gtaatataag ggcgatttat
     4141 gtggatgaat ttcaagatac tagagaatta caatacaatg taatagctaa attacttcaa
     4201 aacaaccctc aaattcagtc aatgtttgtt ggagatattg accaagcaat ttatggatca
     4261 ttagatggta tagctaaatc agttgaagaa ctagaaatgc taactagtca cacttttcaa
     4321 tcaaagacgc tacatggctg ttacaggtct aatcagagat tagtagactt ctactctaat
     4381 tttcagagtt gtccttacga gattgaaagc agagggaata ataaaaacga ccgtggtttt
     4441 ataagttatg attgcaaagt tactaaggag gatttaccca acataataaa aaatataata
     4501 aaggaaaaga ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc tcctcaacac
     4561 tatcctctgt tttcactggg tgagtattta aagaaagaat taccgtattg caattttgat
     4621 gctataaata tatcaccaat taaggtaaat aatcatagtt tatttttcaa gttagcaatt
     4681 ttgtacttta ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc aaacgatatt
     4741 ttaatt
//
LOCUS       DAJSBC010000126         4728 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSBC010000126 DAJSBC010000000
VERSION     DAJSBC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.129"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            68..1114
                     /locus_tag="ONT65_002686"
     CDS             68..1114
                     /locus_tag="ONT65_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2330265.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1131..2354
                     /locus_tag="ONT65_002687"
     CDS             1131..2354
                     /locus_tag="ONT65_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2330266.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2443..3330
                     /locus_tag="ONT65_002688"
     CDS             2443..3330
                     /locus_tag="ONT65_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR2330267.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3416..3646
                     /locus_tag="ONT65_002689"
     CDS             3416..3646
                     /locus_tag="ONT65_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR2330268.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3703..4668)
                     /locus_tag="ONT65_002690"
     CDS             complement(3703..4668)
                     /locus_tag="ONT65_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2330269.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1597 a          788 c          963 g         1380 t
ORIGIN      
        1 atagggctta agtttgagtg acagtttgta ggctatgtta tactatataa gtgaaggggg
       61 acgtaaggtg aaggtattat tatattttga aagtgaaaag atgcttgcaa aatctggaat
      121 cgggcgtgcg cttgatcatc aaaaacgtgc tctgacggaa gtcggtatct cttatacact
      181 agatgaaaaa gaagattatg acatcctgca catcaataca tacggaatca atagtcataa
      241 tatggtcaat aaagccagaa gagaaggaaa aaaagttgtc tatcatgcgc attctacaga
      301 ggaagatttc cgaaattcct ttatcggatc gaaccagctt tctcctatcg taaaaaaata
      361 tctggtagga ttgtaccaaa aagcggacta tctgatcacg ccaacacctt attcaaaaca
      421 gttgttggaa agctatggta tccgtgtgcc gattcaagct atttccaatg ggatcgactt
      481 agagaaatat caccctgatc caatgaaaga acaaaagttc agagaatatt tcaaactgtc
      541 tccggatcaa aaagtaatca tttgtgtcgg tttgtttttc gaacgtaaag gaatcattga
      601 tttcgtagaa attgctaaga agatgcccga atacacattt atttggtttg gtcatgttcc
      661 catgtactcg attcctcgga acatccggaa aattgtaaaa gaagatcatc ctgaaaatgt
      721 cctgtttcct ggttacattc gtggagagat catagaaggt gcgtattcag gtgcagatct
      781 attctttttc ccttcttatg aagagacaga agggatcgtt gttttagaag ctttagcaag
      841 caaacaaaac gtacttgttc gagatattcc agtgtataat ggctggttag aagatagcaa
      901 gaactgctat atggggaaga caaacgaaga atttatccga ctgatccaac agattaccaa
      961 tcaagaacta ccgagtacga cacaagccgg ttatcaaaca gcaaaagagc gaagtatcaa
     1021 taaaatcggt gaagaactta aaaatgtata cgaaagtgta ctgaatgaaa aagtctctag
     1081 caaattaaaa aaagtaaatc aggtcaagga ctagtttgga ggcgaaaaac atgaaaatcg
     1141 gcttttttac cgatacctac tttccgcaag tcagcggagt tgctacatcg atcaaaacat
     1201 taaaacaaga attagaaaga catgggcatg aagtctatat ttttacaacg accgatccta
     1261 atgcggataa gttggaaaaa gatgtgattc gaatgccaag tgtaccattt atttctttca
     1321 aggatcgaag agttgttgtt agaggcatgt ggtatgctta tttggttgcc aaagaactag
     1381 aactagatct tattcatacc catacagaat tcggagcagg gatacttggg aagatggtag
     1441 ctaagaagtt gaaaatacct ctgatccata cttaccatac gatgtatgaa gactaccttc
     1501 attatatcgg taaaggaaaa gtgatcaggc aatcccatgt aaaatacttg tctagattat
     1561 ttgcaaatca tacgacaggt gtagtttgtc caagtgaacg tgttattgat acattacgca
     1621 gctatggtgt aatagcaccg cttcgcgtga ttcctactgg tatcgacatt gaaaaattca
     1681 aacgttctga tatcactcag caggatatca gcaaattaag ggagtcacta ggtcttcagg
     1741 aaaaccattt gatgattctt tctttaagca ggatttctta tgagaaaaat atccaagcct
     1801 tgattaatgg gtttcctcag gtcatctccg cttatcctaa cgccagatta gttatcgtag
     1861 gaaaaggacc ttatgtagaa gagttagagg aattgatctc agaactgcag ttagaagaat
     1921 ttgtgcagtt cacaggagaa gtggacaaca atgatgtagc actttattat aaagcggcag
     1981 attattttgt cagtgcttct acttcagaaa cacaagggct tacttataca gaagcgatgg
     2041 cagccggaac accttgtgtc gtggaaggaa atgcttattt gaaccgattg ttcgatcatc
     2101 cttcattagg gaaaactttt gaaaccgacg aagactttgc accagcactt attgattata
     2161 tttccagtga agttcctaaa gatccaaaag ttttagaagc taaactttat gatatttcat
     2221 ctacacattt cggcgaagct atgatcgaat tttatcaaga catggttgtt tatcatgaag
     2281 aagagatgcg aagaaaagaa gaagaagact cgattgaacg aatcaaaata aaattaaatt
     2341 catttaaaag ataaaattca aacatttttt atgaaaaaat ccttgtttct agcgtatact
     2401 agcatagaag caaggatttt tgatttttag gaggtcgaaa aaatgaaact tggttttatc
     2461 ggaacaggcg tcatgggaag tgctgtcgca cgccatctac tagaagcagg acacgaagta
     2521 gctgtctata atcggacaaa agcaaaagct gatcctttag taacagaagg agctatctgg
     2581 gcagatacac caaaagcggt agcagaacaa agtaatattc tctttacaat ggtaggttac
     2641 cccaaagatg tagaagaaat ctactatgga cagtcgggga ttttttctgc agatatctca
     2701 ggacatattt tagttgattt gactacaagt acaccaagcc tggcagaaaa aattgcaaaa
     2761 acagcaaaag aaaaaggcgc agatgcgtta gatgcaccgg tatccggtgg tgacttagga
     2821 gcaaagaatg gtacactgac gatcatggtc ggtggagaag aagccgttta tgatcaagta
     2881 ctgcctttat tcaaagaatt tggtacgaca tttacattgc atggttctgc tgggaaaggt
     2941 cagcatacaa aaatggctaa tcagatcatg attgcaggta cgatgacagg aatgacggaa
     3001 atgcttgtct atgcacaaaa aaacggactt gatttaaaga aagtgattga gacgctttca
     3061 ggtggaagtg cagctaactg gtctttaagc aattattctc ctcgtatttt aaaagaagat
     3121 tacacacctg gtttttttgt caaacatttt atcaaagatt taaagattgc attagaagaa
     3181 gcagaaaaaa tggatcttgt attgccagca actacccaag cattgaaact atatgaagag
     3241 ttggcagata aaggttttga gaatgacgga acccaagctt tgatcaaact ttggtggcct
     3301 gaaggaaaaa taccagaaaa aaaatcataa aaaacgagtt tttttagaga aaatcaatgc
     3361 aagtaggcta tgcttttgat acaatataac agaaataaga aaaggaggac ctccgatgac
     3421 acattcacaa ttagtcgcaa tcataaaacg actggaggct atggtcgagt cagcagataa
     3481 tgaactgcaa gttcgccgtt ttgaaaaaga aggcgtagag aagtgtacag taacttatga
     3541 taaagcgacc gatacatttg aattaactga aacagatact catcagcaat acgaatttga
     3601 taatatcgat atcgtagcga tggaaatcta tgacttaatc caataaattt taggataatt
     3661 gacatttgaa gttgaagatg cctaaaagaa gccgagaagc aactaattta gttgcttttc
     3721 agcttctttt ttgtttaaca gttcaatttc aaactgttct ttttctaatt ttttgcggta
     3781 actgatcaag atttgtacag gatcgatttc ttcttttgac aattgacgtt tcaaagataa
     3841 atgagcttct ttcttttcat aatagaagtc aggagaaacg tcaccagagg taatcattgt
     3901 ttctgcaagt tgaccataac aaacgaggta gtgctttttc tgactctcat ctgctgctaa
     3961 tggcatgctg aaggtgagcg atggtgttct taaaaactgc ttttgttcct ggatccgtct
     4021 aataaaataa gtaactaatt cgatatcttt ttgaagcata ccggcttttt cgaaattcaa
     4081 ctgatagatg aaatgttgtt gtcttttttt caagtaagtc agtagctttt tatttcggcc
     4141 ttgaaacagc ccttggagtt cctttttttt ctgttcaaaa gtcatttcct gtaggtcagg
     4201 aagctggact cgcaaagcta acaaactaat ttcagacaac caaggcattt gataagtttc
     4261 ttccattata cgcaaaatag acggcatttt tttatagaga cgaaaaggcc cgtatgttcg
     4321 agccgtagga gtatctgtta atacaaatcc cttattggta atatgtacat aattataatt
     4381 actgaaataa ttcatttgtc ggttataaaa aggacggtaa tgttggatca attgacactc
     4441 gagcaataaa gcatctaatt ccgtgtctac tacgacgaaa tcaaaatctg ttatatgatg
     4501 aatcatacgt aatacttttt ttgaatgctg tttattctga tgaaaatagc tgcttactct
     4561 atttttgagc cgttttgctt taccaacata gatgacctta ccatctcggt ctttcataag
     4621 atagacaccc ggcagcatgg ggagagtttt cattttttct tttaacatag gatctccttt
     4681 tacgctttga ataaaaagac tataacaaat tttgaaataa tggacaag
//
LOCUS       DAJSBC010000127         4689 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSBC010000127 DAJSBC010000000
VERSION     DAJSBC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.130"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            106..357
                     /locus_tag="ONT65_002691"
                     /pseudo
     CDS             106..357
                     /locus_tag="ONT65_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ONT65_002692"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ONT65_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2330270.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ONT65_002693"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ONT65_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="ONT65_002694"
     CDS             1786..1869
                     /locus_tag="ONT65_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2330271.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT65_002695"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT65_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2330272.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ONT65_002696"
     CDS             complement(2994..3857)
                     /locus_tag="ONT65_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCR2330273.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ONT65_002697"
     CDS             complement(3859..4131)
                     /locus_tag="ONT65_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2330274.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="ONT65_002698"
     CDS             complement(4148..4357)
                     /locus_tag="ONT65_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR2330275.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4434..>4689)
                     /locus_tag="ONT65_002699"
     CDS             complement(4434..>4689)
                     /locus_tag="ONT65_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCR2330276.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1440 a          794 c          861 g         1594 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attctattct
     4441 ttttcgtttt ctaattgaat aattcgctca agcatttcaa aaaatacgtt cttatacatg
     4501 gataaaactt ttttgtcata gcctttatgc tctgtaatgc cgtttttaga ccaggtactt
     4561 actttattac ttcgcttgat aatattttgg aaaaccaagt tatcttcttt atgttgctta
     4621 tacagttctt ccaggtttga ttttatcgtt gcgctgtccg tatcaactaa ataaggaaca
     4681 aaaccgatc
//
LOCUS       DAJSBC010000128         4635 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSBC010000128 DAJSBC010000000
VERSION     DAJSBC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.131"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            175..519
                     /locus_tag="ONT65_002700"
     CDS             175..519
                     /locus_tag="ONT65_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR2330277.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ONT65_002701"
     CDS             520..939
                     /locus_tag="ONT65_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR2330278.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ONT65_002702"
     CDS             complement(1031..1381)
                     /locus_tag="ONT65_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330279.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="ONT65_002703"
     CDS             complement(1347..2567)
                     /locus_tag="ONT65_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR2330280.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="ONT65_002704"
     CDS             2933..3499
                     /locus_tag="ONT65_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2330281.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="ONT65_002705"
     tRNA            3644..3731
                     /locus_tag="ONT65_002705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="ONT65_002706"
     CDS             4065..>4635
                     /locus_tag="ONT65_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCR2330282.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DAJSBC010000129         4608 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSBC010000129 DAJSBC010000000
VERSION     DAJSBC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.132"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..337)
                     /locus_tag="ONT65_002707"
     CDS             complement(83..337)
                     /locus_tag="ONT65_002707"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2330283.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(448..702)
                     /locus_tag="ONT65_002708"
     CDS             complement(448..702)
                     /locus_tag="ONT65_002708"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330284.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /locus_tag="ONT65_002709"
     CDS             complement(895..1170)
                     /locus_tag="ONT65_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR2330285.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="ONT65_002710"
     CDS             complement(1142..2095)
                     /locus_tag="ONT65_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2330286.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="ONT65_002711"
     CDS             2707..4200
                     /locus_tag="ONT65_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR2330287.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4608
                     /locus_tag="ONT65_002712"
     CDS             4335..>4608
                     /locus_tag="ONT65_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2330288.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DAJSBC010000130         4470 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSBC010000130 DAJSBC010000000
VERSION     DAJSBC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.133"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            318..1049
                     /locus_tag="ONT65_002713"
     CDS             318..1049
                     /locus_tag="ONT65_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2330289.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="ONT65_002714"
     CDS             1194..3221
                     /locus_tag="ONT65_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2330290.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="ONT65_002715"
     CDS             3329..4426
                     /locus_tag="ONT65_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2330291.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          923 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt
//
LOCUS       DAJSBC010000131         4458 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSBC010000131 DAJSBC010000000
VERSION     DAJSBC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.134"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..115)
                     /locus_tag="ONT65_002716"
     CDS             complement(<1..115)
                     /locus_tag="ONT65_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2330292.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="ONT65_002717"
     CDS             246..698
                     /locus_tag="ONT65_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330293.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="ONT65_002718"
     CDS             712..1401
                     /locus_tag="ONT65_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330294.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="ONT65_002719"
     CDS             1526..3877
                     /locus_tag="ONT65_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR2330295.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="ONT65_002720"
     CDS             3957..4352
                     /locus_tag="ONT65_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330296.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1567 a          573 c          885 g         1433 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tatttaat
//
LOCUS       DAJSBC010000132         4425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSBC010000132 DAJSBC010000000
VERSION     DAJSBC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.135"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            690..1658
                     /locus_tag="ONT65_002721"
     CDS             690..1658
                     /locus_tag="ONT65_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2330297.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ONT65_002722"
     CDS             1669..2484
                     /locus_tag="ONT65_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR2330298.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ONT65_002723"
     CDS             2552..3262
                     /locus_tag="ONT65_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2330299.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ONT65_002724"
     CDS             3298..>4425
                     /locus_tag="ONT65_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2330300.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAJSBC010000133         4375 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSBC010000133 DAJSBC010000000
VERSION     DAJSBC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.136"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..1350
                     /locus_tag="ONT65_002725"
     CDS             49..1350
                     /locus_tag="ONT65_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2330301.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1377..1637
                     /locus_tag="ONT65_002726"
     CDS             1377..1637
                     /locus_tag="ONT65_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2330302.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2636..3871)
                     /locus_tag="ONT65_002727"
     CDS             complement(2636..3871)
                     /locus_tag="ONT65_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2330303.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1278 a          694 c          895 g         1508 t
ORIGIN      
        1 ttgtcatgca atgaatggtt catattttat tgaaaaggag tggttttagt ggaaaagctg
       61 atgaaatggg tcgaagagaa acttgtccca ccaatggcaa aaattggtac acagcgtcat
      121 ttattagcaa ttcgtaatgg ggttgtttca acattgtcat tgattttgat tgggacattt
      181 tttatggtct ttatcaattt accgtttcct ggatggaatg aatttattgc cccttattcg
      241 gcaacgattg ttttaccctt taggatgacg atgggattga tggctattta tgcaactttt
      301 gttatgggtt cggatctagc taaatcatat ggattagata gtgtgacagg tggtatatta
      361 tctttaggaa cgttctttat gttacaagtt cctgtaaacg ttttaactcc tgaagaggca
      421 cctcttggct gggtcttacc tatgagttct ttgggagcat ctgggatgtt tgcggggatc
      481 ttatctatga tttttgcagt ggaagtatat cgtttcttta aaaatcgaaa tattactatc
      541 aaaatgccag aacaagtgcc acctgctgtt gcccgtagtt ttgaggcatt gattccaggt
      601 gcagtcattt taacaattac ttggctgatt cgaagcgtcg cgggctttga tgtaaacgct
      661 gccttattat cgttattcga gcctttaacc aatattttag ggaataattt gcttggtgtt
      721 ttactaccaa tgtttttgat ccatttgttg tggtcatttg gtatccatgg catgagtatt
      781 gtaggggctg ttgttcgtcc aatgtggctg atcatgttgg atgagaatgc aaaggcattg
      841 gcagatggta cagctgccac ccaattaccc tatatcgctc ctgaacagtt ttatcaatgg
      901 acggtaacaa ttggtggtgc aggagcaacg atcgtggtat ctgttttgtt tatattcttc
      961 tgtaaatcaa aattcttgaa agaggttggt cgtttttcta ttattcctgg tatcttcaat
     1021 atcaatgagc ccataatttt tggagctcct atgatgttga atccttacat gttcatcccg
     1081 ttcaacttag ttcctttagt tttgactatt atcagttatt ttgcagtcaa attagaaatg
     1141 gtcaatagat ttactgtttt gccaacatgg acattaccag ctccaatcgg cggttatctt
     1201 tcagcaggaa atgactggcg tgttgttatc ttgatcatga tcaacacatt gatagcattt
     1261 attatctatt atccatttgt aaaagcttat gataaaaaga tgctgtcaga tgaacaaagt
     1321 cgtggagaaa acgaagtcca gactgtctaa gtttggtttt tagaaggtgg gaaacaatgt
     1381 attggctgat tttatttttt gtttttatct ttttattaac ggctagtcac cttatattga
     1441 atatgctagc tacatatcat attcaaatca atcgatggat ttgggcactg gcatcatttt
     1501 tgatcgtgat tcttccaaag atcattgtac cgcatatgaa tgttttgttt tcatggggga
     1561 catatgtact ttgtggaatt tttgctatca attttatgat cgagcagcat agatggtttg
     1621 tcacatcgaa gctttaaatg ttctaaaagt ggttgtaaca gaagtggtta gcttcaagaa
     1681 ataagaagaa atttacgaaa attgcttctt aaatttttgt gaaatgttgg cttatttttc
     1741 gtttggttga acttattacc gcagatgcaa gcttttgacc accgtttatt cggaatatgg
     1801 gactgtgaca aggcttttgt cacagccctt ttttacataa agattattat tgttttaact
     1861 gttttggcag aaagaaggag gaaaaaatgg aaattttatc agcaatggat ttagaattag
     1921 tagatctact accaaagtga tcaagtcaaa tatagatttg ggagcttaca accatatagt
     1981 aggggctgca gtatggtgta tgaatggtaa tatgtatgat gtgagtgttt attcaaatca
     2041 tgaaacgtgt atagaaataa ttgtgctggg aatagcaatc agtcatggtg aacgtgactt
     2101 ttagtgtatc gttgctgacg acatagaaca ttctgacatg atctactcaa tatgtgggga
     2161 ttgctggcag tttatatagg agtatgcatc ggattgcgag atcattgtct caacagaaga
     2221 gggaggttga ttatcaaaga taaagtggta aatcctacat ttgatttagt aattgttgag
     2281 gggactagcg gcacaaatct ctacaaagcg atgcttctag atctatatga cacattttaa
     2341 gaaaatgtat ttgcctaatt attatggtct ttcttttgaa gaaacgttga gatgatccaa
     2401 ccagagaaag gtgtcaaaga atttgactaa gaataaatgg cagacgctac ctatgctcat
     2461 ttggatagcg tcagatcttt tattttaagg atgattttgt gttttttaga caccgtaatt
     2521 taagtaactc taacttttca atccactatt tacttttttt gtattttcac tgtctgggtc
     2581 tcgaatagcg aaattactga tgagtctcta ttaaaaaagc tttttctact tgaaactata
     2641 ttgggtggtt gctcaggaag gtttcgattt cttgaattgc ttgttgtgga ccatttattg
     2701 gaaaaacggc ttgcagcgtg cgtgcctgct gacgaatctt gggtgaagaa acttcgcgca
     2761 gagcaatctg cagttttctt tcagtcactg gttttgtgag ggcgattgcg tttccgtatt
     2821 ttacacaata gtcaatatta cagctttgct caaattgcat ccccacaccg ataaacggtt
     2881 tgccggattc acaggcagtt tggacggtcc cttccccgcc gtggataaaa gaaaaatcaa
     2941 tgaattcatg caaaagatga ctagacagat attctgttat taaaagatct tctgagtcag
     3001 attgaatgtc atcaagcaga tagtcaatag gtgcaataac cgctagatcc ggttgttttt
     3061 gtaagaagaa caaaattttg cgtaacatac gcggattgcc tgaactaccc attgcgaaat
     3121 agataatacg ttttctttct tgttgctttc gctgtataaa agttagcact tcttcaggca
     3181 aggaagtact gagcttagca aaaattggac caacgattcg ataattttcc ggcagctgtt
     3241 cttcttccaa aaatagttta ggcgttgtga tgagatttag atcagcatcc atcatttgta
     3301 aacttgtttt tggtaaagga agcatttctt tttgtgcagc gtgtttgaag gcattgggga
     3361 tccatcgggt atttactata cgcttttttg tccagtgcca aaggggattg cttaaagggc
     3421 cgaagaaacg taactcgtgg ggcatcagcg catgctctga ttgaaaaaaa ggttcactca
     3481 aagcataggg tttaacatat actaaaggaa tctttttatt gcgggccgat aaaaaaattg
     3541 tcacattcga acctgtcaca atcagtcgag gctcaaactt agcaatcaga ctctgttcac
     3601 tagtgatacg agaggcaacc atttctttgg taaaagggtt tttaagtgta cgcaactggt
     3661 caaattttat gatttgttct tcttgttttt tcgtaaggat cggcgctagt aaatgatact
     3721 caaagccact ttctgtgatc aaaggagcaa aagttttaga aaagccaaag aatacacagt
     3781 aataacggtc tttcatagct ttggcaacct caatcattcg tgttgtctca gctaaattga
     3841 atactgcagg agcaaaaaga attgtcttca tgaaatcctc cttattggaa tgagtctatc
     3901 ccttcgtttc ttactagtat actaaaaaac acagcgttcg agtagcctat gtatattttg
     3961 aatattaaac tgtctctcgc tgttttcaca acttctaaat ttaaattttg taatcaagtt
     4021 gaaactccaa ttttcaaaaa aacacgcaac aattttttga tcgatttata tgttgtgcca
     4081 gataaatacg agaagttgtg tcattaaaaa taaacatgag aacataaaaa aataaaataa
     4141 ttatattaat tgataggtgt gctgtcgtca ttatacacac gtaagaaaga aatagttatg
     4201 tttaaaaata ttcttagact ttctcgccat gacaattttc actatgtgtt ataactgtaa
     4261 acgataatta ttcagataac acaaaaagaa atcaaggagc agatacgcca gttgtttaat
     4321 ttgaatatta ttttaaaatt aaaattttta ttctgttcta aacatttttg gttgt
//
LOCUS       DAJSBC010000134         4345 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSBC010000134 DAJSBC010000000
VERSION     DAJSBC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.137"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(387..1853)
                     /locus_tag="ONT65_002728"
     CDS             complement(387..1853)
                     /locus_tag="ONT65_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2330304.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT65_002729"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT65_002729"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2330305.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DAJSBC010000135         4310 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSBC010000135 DAJSBC010000000
VERSION     DAJSBC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.138"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(65..1285)
                     /locus_tag="ONT65_002730"
     CDS             complement(65..1285)
                     /locus_tag="ONT65_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR2330306.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ONT65_002731"
     CDS             complement(1305..1916)
                     /locus_tag="ONT65_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2330307.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ONT65_002732"
     CDS             complement(1959..2891)
                     /locus_tag="ONT65_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2330308.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ONT65_002733"
     CDS             complement(3312..4172)
                     /locus_tag="ONT65_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2330309.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAJSBC010000136         4203 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSBC010000136 DAJSBC010000000
VERSION     DAJSBC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.139"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..354)
                     /locus_tag="ONT65_002734"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ONT65_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ONT65_002735"
     CDS             complement(614..1162)
                     /locus_tag="ONT65_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330310.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ONT65_002736"
     CDS             complement(1163..2020)
                     /locus_tag="ONT65_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2330311.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="ONT65_002737"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="ONT65_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="ONT65_002738"
     CDS             complement(2582..2911)
                     /locus_tag="ONT65_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2330312.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="ONT65_002739"
     CDS             complement(2988..3260)
                     /locus_tag="ONT65_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2330313.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="ONT65_002740"
     CDS             complement(3260..3523)
                     /locus_tag="ONT65_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2330314.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="ONT65_002741"
     CDS             complement(3629..4045)
                     /locus_tag="ONT65_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330315.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="ONT65_002742"
     CDS             complement(4051..>4203)
                     /locus_tag="ONT65_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2330316.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DAJSBC010000137         4072 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSBC010000137 DAJSBC010000000
VERSION     DAJSBC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.140"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(19..477)
                     /locus_tag="ONT65_002743"
     CDS             complement(19..477)
                     /locus_tag="ONT65_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2330317.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="ONT65_002744"
     CDS             complement(490..1821)
                     /locus_tag="ONT65_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR2330318.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="ONT65_002745"
     CDS             complement(1832..3274)
                     /locus_tag="ONT65_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2330319.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="ONT65_002746"
     CDS             complement(3261..4025)
                     /locus_tag="ONT65_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR2330320.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         1225 a          881 c          603 g         1363 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tc
//
LOCUS       DAJSBC010000138         4071 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSBC010000138 DAJSBC010000000
VERSION     DAJSBC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.141"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(180..1685)
                     /locus_tag="ONT65_002747"
     CDS             complement(180..1685)
                     /locus_tag="ONT65_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2330321.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1682..2368)
                     /locus_tag="ONT65_002748"
     CDS             complement(1682..2368)
                     /locus_tag="ONT65_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2330322.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2561..3982)
                     /gene="gndA"
                     /locus_tag="ONT65_002749"
     CDS             complement(2561..3982)
                     /gene="gndA"
                     /locus_tag="ONT65_002749"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR2330323.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1120 a          859 c          694 g         1398 t
ORIGIN      
        1 attacagcat tttattgttt tttcgttcat ctaaaagata cttacattta attaacggat
       61 gttattctat ttaattgttc gctagttttt ttataatgac atccctgaat ttctcacttg
      121 cttgtatttt gtaccttatt ctttgttgga tatataaaaa aacaagtaaa gcacatgctt
      181 tacttgtctt ctgcctttgc gataggaata aagattctaa aaattgtccc ttgccctact
      241 acactttctg ctaagatacg tcctttatag ttttcaacca gttgtttagc gatagacaac
      301 cccagaccat ttccaccctt cgttctagct cttgctttat ccactcgata aaaacgattg
      361 aagatcttat taaggtcttc tttgggtatt ccttcgccaa agtcctgaat cgcgatctca
      421 aattcattca gagtggaaga aatagagata tggacttctt ttcgtgtagt tgaatacttt
      481 actgcgttat ccaatatgat aatgattaat tgttcaaagt gattacgata aatttgcagg
      541 gtcacttctt ttgtcaaatc atcatctaaa gtgatcacat agtctggata taatatcttg
      601 aaattattat aaacttgata ggtgacttct tttcctttag ttgtttcatt cgcataatga
      661 acatctactt gttctgctct tgataagtct agcatctctt gaactaagct tttcatacgg
      721 ctgatttctt gcatgctggc gcttaatgat tcttctaata tctcaggatc atctttcccc
      781 caacgattta gcagactaag atgcccttca ataattgcaa caggtgtcct taactcatga
      841 gatacgtctt ccacgaattg ctcttgttgt tcaatataca aacgcatacg gtcaagcatc
      901 tcattgaata tttcagctaa atctgccaat tcatcatttg tgtcgatttc aggcatgtgg
      961 atatctgatt gcggatcttt acgtatagta tccattgtat ctctgagtac cttgagtggt
     1021 tttaagaaat aagaagataa aaagaaacct aaaatactac ttaagatcaa agaaacaact
     1081 tctaaaacaa taagagtcaa taatagctta tttcgaatat catagaaaga agataattca
     1141 tagaagcttt gcgcatagcc gatcttttct cttgtacctt tagaatatat aggctggata
     1201 gataaaaagc cagtttttcc ttctagtgta acgatcgttg gaactttacg tgtagtctga
     1261 atcaggtttc gactgttttc atgggtttta aaaatcaagt tctcgtccaa atcataaaca
     1321 taaagatcca ttgatggctg ccctagctca gctataaagg aatcgatctt cattaagttt
     1381 ccttcgacca gtgaagtatg atctacgacc tcatctccgt catttgtcgc tttcgtcaga
     1441 ttacgatacg tatttactaa tgtcagttct tcatccgagt tagccaaacg agaagctact
     1501 tctgaaatcg tacgctctac attgtttctt tcttttgcca caatcagatt gatagatgat
     1561 ttataggtaa tgacagcgaa aattgtaaac acaacgaata tgaagaaaga actcgcaaag
     1621 gcccacttga ttgtcaagga gggccctttc agttctcttt tttccgattt tttcggcttt
     1681 atcacgagcg catcacataa cctgttccgc gtacagtttg gatatagctt tcctccccgg
     1741 gaacgtcaat tttgttgcga agataacgaa tatatacgtc cactacgttt gtttcaactt
     1801 ctgtttcgta tccccacact ttatttagta aaacgtcacg tgctaatacc acgttcacgt
     1861 tttccattaa agtaagcagc aactcgtatt ctctttttgt tagttcaatt acttcatttc
     1921 cgcgacgtac gacacggttt tctttttcga tagtcaaatc gcgataagta atagtagttt
     1981 gtttagccac atttttatcg ccttcaatat cgattcgacg caacaatgca cgaagacgtg
     2041 ctaataattc ttcgatagca aatggtttga cgatgtagtc atctgctccg tggtcaagtc
     2101 cagatacacg atcgatcaca gaatctcttg cagtcatcat aatgattggt gtatttttga
     2161 cttggcgtac acgtctgcag acttctaaac cgttcaattc tgggagcatc agatccaaca
     2221 atatggcatc ccactcatta tttaatgccg catctaaacc agttcggccg ttgtagtgga
     2281 cttctgcgtt atacccttca tgcttcaact ctagctcgac aaatcgtgcc aagttctttt
     2341 catcttcaat gattagaata ttgctcattt attattttat cctttctatt ctactggctt
     2401 tttaaaaact ctatcattat accgataata tgcttaaaaa tctatagtca ttttatcatg
     2461 gatagcaatt ttaaaaaaga gctgaagtct actcataaca atcgacttca gctctttcgt
     2521 cctcttttta ttttctcatt tcctaacagc cgaaagacta ttattcttca tggtaccatg
     2581 agtaatggaa gattccttct ttgtcgacac gctcataggt atgtgcgcca aagtaatcgc
     2641 gttgggcttg gattatattt gcaggcagac gttctgctct gtatgaatcg aagtaagcga
     2701 tggctgatga gaaagtcggt acaggtacgc cagcttggac agccaaggca acgacgtctc
     2761 tgactgattg ctgatatttt ttcgtgattt caacgaagta atcatctagc aataaatttt
     2821 cgatctcagg atttttgtca taggcatccg taattttttg caagaatcga gctcggatga
     2881 tacatccagc gcgccagatt tttgcgattt caccaaatgg caagtcccaa tcaaattctt
     2941 ttgaagcgac acgtaattga gcaaatcctt gggcataact catgatttta ctgaaataca
     3001 acgcttctcg gattttttcg accaattctt ttttgtctcc cgtgaactca aaatcgttcg
     3061 tacgagacaa aatcttgctt gcttgtacgc gttcttcttt ataagcagaa atatagcggg
     3121 caaatacaga ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg ctttggcttg
     3181 tccatttacc tgttccttta ttcccagctg catcaaggat cacatctact actggtttac
     3241 ctgttccttc atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc aaatagctgt
     3301 ctaattcgcc ttgattccat tctttaaaga tatcagccat ttcttctact gaaaggttta
     3361 aaatattctt cattaagtca taagattcag caatcagttg catatctcca tactcaatac
     3421 cgttgtgtac cattttcaca taatgtccgg ctccatttgg gccaatatat gtgacacatg
     3481 gtgctccgtc ttctgctttt gcagaaattt gctctaagat tggtgcaact agcgcatagg
     3541 cttctttttg tccaccaggc atgattgaag gtccttttaa agctccttct tcaccaccgg
     3601 aaacgcctgt accgataaag ttgatgccag agttggctaa ctcttcatta cgacgcatcg
     3661 tatctttaaa gaatgtattt cctccgtcaa tcaaaatatc gcctttatca aggtgaggaa
     3721 gtaactcttg gattgttgca tctgttgccg gtcctgcttt gaccattagc aagatccgac
     3781 gtggtttctc gatagattct acaaattctt cgattgtata tgttgctttg aaatttttat
     3841 ctgggtgttc ttctactaca gcggttgttt tagcccctgt acgattgaat aaagccactg
     3901 aatagccgcg gctttctatg ttcaaggcta gatttttacc cataacggcc ataccgacaa
     3961 cgccgatttg ttgttttgac atatgaactc ctcctacata cgggatttct ccctaatctc
     4021 ttacattata gcttaacacg gctacattgc aaactaaaga acaacatatt t
//
LOCUS       DAJSBC010000139         3987 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSBC010000139 DAJSBC010000000
VERSION     DAJSBC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.142"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..938)
                     /locus_tag="ONT65_002750"
     CDS             complement(<1..938)
                     /locus_tag="ONT65_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR2330324.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
     gene            complement(1160..1522)
                     /locus_tag="ONT65_002751"
     CDS             complement(1160..1522)
                     /locus_tag="ONT65_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330325.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1526..1864)
                     /locus_tag="ONT65_002752"
     CDS             complement(1526..1864)
                     /locus_tag="ONT65_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330326.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1843..2262)
                     /locus_tag="ONT65_002753"
     CDS             complement(1843..2262)
                     /locus_tag="ONT65_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330327.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2255..2488)
                     /locus_tag="ONT65_002754"
     CDS             complement(2255..2488)
                     /locus_tag="ONT65_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330328.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2491..3459)
                     /locus_tag="ONT65_002755"
     CDS             complement(2491..3459)
                     /locus_tag="ONT65_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2330329.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
BASE COUNT         1131 a          892 c          549 g         1415 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
      121 tgcctataaa tatacctcaa ctcatcagaa acgctctcac gctcttttac agccgtttgg
      181 cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt aattttgttg
      241 gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat agagtcaact
      301 tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc gtgtatccct
      361 tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt ataaccttca
      421 gcaaccggca caatattcaa gtgcaggtgc ggacttattt cgtcattatg aataacagca
      481 ttataaacag ccaaattagg gtttctctct tgaaactcct tgtaatactg aaccaaaatt
      541 ttattggcaa tcactcgatt ttgttcagct tgttgtttcg atatgccagt agaagaacca
      601 tctgctcgat ttgtctggaa atcatctaaa atgcctattt gcacaataaa ttctcgttga
      661 ggttccaact ttttatcctt gcgcactttc gctaaatagt cctcgatttt tctatcggct
      721 cttttttgtt tcgcattata tttttctacc acttcgccaa acaattcctc atatttatct
      781 gctagattcg tctgctctaa ataaatattt tcatccgatt tcgtgaagtc aatgtgctta
      841 tgccaatcat tggttttctg gcgatcagtt aactcacgat tattatgaga aatacttgta
      901 ttcttcgttc cttttttaaa tgaaatacta aaccccaagt taaaaccctc cttcgcgctg
      961 tcgctaataa aaagattgta aacctttctg ttactctttg tcaaagagta acattttcct
     1021 aattgtgaca tggatgtcac aattccaaac gctctccgag ggttttcgct gtcacaagtg
     1081 acaacgaaaa aaggagccga aagcgtatca cgctatcaac tccttttatg ctttattttg
     1141 cttcacgcaa aataatcgct tatcgtaaat tcacgtcatt aaatattgac gtaaatttac
     1201 tctcactcat ctgacttttc ttattcggta tcactccgaa taagtcgtca gattcatcgg
     1261 gataatctcg ttcgcttggc gctgattttt catcagcaag ccactcatta tccactgttt
     1321 tttcgtgttg ctgatcgtca gaaattgacg ataaccaccg ctcaaaatca gcaaaagttt
     1381 caaacccaaa acgcttgcga agagccttgc ccattgcagc attttctttc tcaatcaagc
     1441 gttttttctg ttggcgaagt ttcgcttgtt ctttttttaa tttttcttct tgcgctttaa
     1501 tatctgctaa agtcattccc ataaactaca tctccttttc tttggaatta ataacggaaa
     1561 tttggtttaa ctgcataaca tgactcatta atttcaacca aaattctttt tctgattcat
     1621 cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa gattttgcta
     1681 gatcagcatt gataaccgag ttgagaacat ccataattcg ttcaagcatt tcactataat
     1741 tatcttcatc cggcaaattc aaataatcag ctaaccgttc atacatcaat tcaattctat
     1801 cgactgtttc ttctctcaaa tcaattttat ccatgtcaca cctcacttcc agaattgcca
     1861 ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt tttgttgctg
     1921 aagcaaagtc aatttttgtt ggtcacgaat catttccgtt aattcagaaa tatgcctatc
     1981 tttttccgct aattgttcac gcaaaaattc attttctttt tctaacggga aatcattgcc
     2041 tgcatcattg accggtaaat caccagtcaa ttgaccacct tttgaccgat aaattgaccg
     2101 cacaacacca atcaattcct tgtctaacat gataacccca ttatctttat accgagcaag
     2161 gtcataacct gcattgacca gttgattgcg cactgtattt ttagatatac ccaactcatc
     2221 agcaaggttt tttatggtca ttttttctgg caaatcagtc attatcttct ccgttacttt
     2281 cagctacccc ataaacatat ttaacagcat gcttagggat aatgattggg gaatcaattt
     2341 tattattttt caaaatcaac tcatactgcg ttacatcaag taacttaccg gacatttttt
     2401 tgctatccaa aaaaacaacg acaacgttat cacctgttaa attcaccaac caatttttct
     2461 taccaaaatg cttttgtttt tctgccatta ctattcctcc tctgtcacat caagtccctg
     2521 taaccaagta tccaattctt tttttaaatc atctgctgat tcttcaaatg tttctcgtaa
     2581 atgattcgtc aactcatcat tttgactttc tcgctttaat aaatttcgat atttatattt
     2641 cgagtttctt gacaaaatcg cccaattaga atgattagct aacaatccat caatcataac
     2701 tttatccgta ggttttatat cagttggtaa aaaatgtgga ctcacaaagc tatccaaact
     2761 ttctttcacc atagcatacc attccgtagc tttaccacgt cttaactgca tttctaaccg
     2821 ccaccaagta ttaatttctg aaggcaccac ctttcttttc atttcttgct ctattttttt
     2881 attgtacaaa cgaatttgcc gctcgcttgc ccgactaccc caatacaccg tttccagttt
     2941 tcctgagcgt ccataaatta atttaaatga aactggatca ataattcgat attgcgtgat
     3001 aaactcatca ggcacatcaa taagatcgca ggcaacatcc gctcgtgaaa aatggggctc
     3061 taaaaataaa tcatgaataa aatttttcat ggaatccgct aaaaatcgac tgattttatt
     3121 aggattaaaa tcaatccgcc cttttccttc ttgccattta gaaatttcaa tataggcaat
     3181 attttcgtca tgttggtcta atagctgcca accattattt ccaaccgctt ttaaatagcc
     3241 attgccttca tttacccgca taagctgttc aaaatctaat attaatacat catgttgagt
     3301 atgataaaaa atattttgat tcaactttcc aacaatggta attcggtcaa tcgaccaatt
     3361 taattttata gtttgtacca aagtttgaac atttaaccgc ctgttagaca gggctgttaa
     3421 tttttcccac tcattcacga ttgttctttt tttactcata ttcagttcct cctcaaagaa
     3481 aaaagagatt tttggaaaga atttccagag ttcacgtaac tctaaaaatt cataaccatc
     3541 aatctctagt cttgaagaaa cctaaaaata ttcgtataat gaatttgagt tacggaacta
     3601 gtgtggttag tttccgtaaa ggtcgagaac gttggtagcg ttgctcgact tatttttttg
     3661 ctctttttcc agttcatttg tttgctaact caataatagc acttctcttt agctatgcaa
     3721 ataccaaaaa aataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     3781 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg ggctagtggt
     3841 ttagccgtca actccttatc ctcggcacaa accgcctccg gtaccgttga ctgcacatcg
     3901 tggctaccac tcaatctcat tctcagaacg ctctaattta tgctgtgaga cgttttgacg
     3961 ataaaataag gttttctcct gctctaa
//
LOCUS       DAJSBC010000140         3791 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSBC010000140 DAJSBC010000000
VERSION     DAJSBC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.143"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..83
                     /locus_tag="ONT65_002756"
     CDS             <1..83
                     /locus_tag="ONT65_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2330330.1"
                     /translation="SVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            74..859
                     /locus_tag="ONT65_002757"
     CDS             74..859
                     /locus_tag="ONT65_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR2330331.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            828..1544
                     /locus_tag="ONT65_002758"
     CDS             828..1544
                     /locus_tag="ONT65_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2330332.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(1646..2680)
                     /locus_tag="ONT65_002759"
     CDS             complement(1646..2680)
                     /locus_tag="ONT65_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR2330333.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2684..3742)
                     /locus_tag="ONT65_002760"
     CDS             complement(2684..3742)
                     /locus_tag="ONT65_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR2330334.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         1248 a          758 c          675 g         1110 t
ORIGIN      
        1 aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc aaacgaccgt
       61 tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta cacctctttt
      121 tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag aagtaatgaa
      181 attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag ctcaaaccca
      241 tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga agtatgaaga
      301 agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg gccaagtctc
      361 aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa acgaagccaa
      421 agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa tggagatgga
      481 tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg caaaagaagc
      541 cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact atgacacaga
      601 agctgaagta ttcatcgtga ccgtctgcga tggcggtgtc gggatatcag aagaaatctc
      661 agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt ttggattgaa
      721 tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca aacaaacaaa
      781 tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg
      841 tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt gtggaaagca
      901 tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa aaatatttca
      961 gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa agtggtatcc
     1021 attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg atcactgctg
     1081 cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat tatttgatca
     1141 aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga
     1201 caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc aaccacacca
     1261 ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca aaactgactt
     1321 taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca aaagaactgg
     1381 ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt ttagtagaac
     1441 aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccagccgcta acctgttatg
     1501 aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc gaattatccc
     1561 atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat
     1621 tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct
     1681 catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca
     1741 tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt
     1801 acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc
     1861 ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc
     1921 ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca
     1981 agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac
     2041 ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg
     2101 agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt
     2161 tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga
     2221 tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt
     2281 tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca
     2341 aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa
     2401 aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac
     2461 gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca
     2521 acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat
     2581 aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac
     2641 gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta
     2701 tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag
     2761 ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg
     2821 ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa
     2881 aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag
     2941 gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt
     3001 tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac
     3061 cagctaaata atccatcggc tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg
     3121 ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga
     3181 acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt
     3241 tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa
     3301 agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg
     3361 cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg
     3421 ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa
     3481 tataaataac taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga
     3541 tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg
     3601 atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata
     3661 atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc
     3721 gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa
     3781 aaaagctttt a
//
LOCUS       DAJSBC010000141         3782 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSBC010000141 DAJSBC010000000
VERSION     DAJSBC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.144"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(701..994)
                     /locus_tag="ONT65_002761"
     CDS             complement(701..994)
                     /locus_tag="ONT65_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR2330335.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1397..2575)
                     /locus_tag="ONT65_002762"
     CDS             complement(1397..2575)
                     /locus_tag="ONT65_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2330336.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2679..2993)
                     /locus_tag="ONT65_002763"
     CDS             complement(2679..2993)
                     /locus_tag="ONT65_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330337.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3100..3318
                     /locus_tag="ONT65_002764"
     CDS             3100..3318
                     /locus_tag="ONT65_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330338.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3313..3708)
                     /locus_tag="ONT65_002765"
                     /pseudo
     CDS             complement(3313..3708)
                     /locus_tag="ONT65_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1211 a          721 c          554 g         1296 t
ORIGIN      
        1 aaagatttta tttttaaata aaaaatactc tttttttacg attattgaaa aaaaaagata
       61 aaacttgatt aattgaagta ttgcttaaat ttggaagcta tgagggaaga ataaaagagt
      121 taggacggga ttatctacta tttttctttg ttttaaatat ttgttatctt tgaacataaa
      181 agagaaatgt gtcgactgca gtgttgtgtc cctcatcacg aataaacggt gttcaattag
      241 ctgatcttgt attcacttaa aaaatggtaa agtacgagtt gctattcccc attttcaatt
      301 ttctaacgct caggacaaaa tactgttttc acaacctcat tggaataaaa gcatatatat
      361 tgtttcaaga acgaaatctt gttatctttt taaagggcat ctgtccaaaa tctaatttta
      421 ggaggtacgt tttggtattt attcgtacaa ataaaagaga attatcttta ttcatgtaac
      481 gcatccaatt gattttttca tgtacgctcc tggaacgatt ttactgacga ttgcttattt
      541 aactgcgaat cgctcattag cttttatcat agctatccat attttgaata acgttcttgg
      601 ctttgtatta tagatacaga tgacaaaaaa agttcaagta tgcggtgtag aaccaatatc
      661 gcatacttga acttttttca atgagtataa attaaaactt ctaattttca attcccatca
      721 tctgatatgc ccgaccgtct gaacttcttt tgaaaaagcc aaatgtgatt agataacgtc
      781 tttgttcgac ataatcgcca ctagaaaata cgttcagcag tatcgaatta acctctgttt
      841 ctgtataagt cactccatgg tcaaagcgtt tcaagacttc tttcaatagg atgtatttct
      901 tttttgttga acttggtact tgttttactt ttccatcatt catgaatgtg tctaaaactt
      961 tcatcttttc ttgctccgta taagtataaa ccatcttctt tctccttttg attctaagaa
     1021 tactccattt atgttagaat attctggatt ttttattaaa gcggttactc ttaagaaaga
     1081 tattatcata gtgctctttt ttttactact acttataggt agatttattc taatccatta
     1141 ttctccccta ccaataatta aagaaaaaac tggatcaggg tgttcaaatt acatgctaag
     1201 aaaaaagaac ccttgaatac caagggttct ttctcagtct gataacaatg attaacgacg
     1261 gatttctttg atacttgtga aaagtcgtta aagtattgat ataaaaggat ttttattacc
     1321 tctgactaca tattgactaa tttgttaaaa aatcgttttt caagaatcct gtaataccag
     1381 tgctttcacc gtttttttaa ctctctaaag aactaaaagc aatcagaggt tttacctttt
     1441 catttgacgt taagtggcga taaattttcg aagttgtttg gtcagtatgg cctaagcgat
     1501 ctttcaagac ttcccagcta cagtgttccg ctactaagct tgccattgta tgacgagcta
     1561 aatgagtatg aaatggctta ttaattcctg agcgattcac gcaccgtttg acgtgatcat
     1621 ttacatactc tcgacgataa ggaacaccac gtttactaga atcaagatac acaaacaaat
     1681 atgtttcttc tataataaac ctgtaatgtt gcctcatctc acccaaagcc ttaatcaact
     1741 gttgtagctt ttccataact agaggagtta ctagaactcg tctagcagaa ctaactgtct
     1801 tagtagtttc aattcgaaaa tccttactct tacctttaac tgccgtttgc ttatagaaca
     1861 gaatttcatt tcttttgaaa tctatatctc caggctgtag agcaagcgcc tcgctgactc
     1921 ggcagccagt gtaaaataga acatggaaaa gtacagacat cggtaattcc tcatgaacaa
     1981 tgccataatt caaaaaagtt ctcaattcat cgacagtaag aaacttgtcc tcaatattat
     2041 ttatggcttc ttttaaatca gttaccgtta actgatattt tggcattctc acagacttca
     2101 ttggattttc acttatcaaa ttctttcttt gacaatattt aaaaatcata tttagaactt
     2161 gaataagtga ttgtaaacta tttttggcat agccaacatt tgttccatta acgccacatt
     2221 cctttaaggt aaaaagcaat tcttgaaggc actctggtgt caaatcagac aactttttat
     2281 caccaatcca tcgaacaata atttttaatt gttcctctcg cttatcatag gttatttcct
     2341 tttgttcatt taacgaacgg taatattgga accattgctc acctacaaca ctaaacttgt
     2401 cattgcttgc aacaattttt ttcttaagcc tttgttgttt ctccatttcc agataagcga
     2461 gttgcgcctc acgttttgaa ttaaagccac gtcttgttgt tcgatatgct tgaaagtacc
     2521 atgtgatcct accagtagaa ctgatttgct tcttgaatcg atctgttttt tccatttatt
     2581 gattcctcct attttgaagg aacccaacaa gcagaaaccc tttggaaatc tttctatcta
     2641 tgtcatcatt gtaccgcatc ttagaaagaa aatgtaactt ataaagatcg aagtagtcgc
     2701 aatttttctc gatagtcatt cgaatcactc tttctcatcc attctaaaac atcatcacga
     2761 aaatagtgtc ctcgaatccc gtaccaacag ggaggaaact caccaagttt tactaaacgg
     2821 ttcagtgtac tatcacttat ctgaaatagt cgctttaaat cagtatttgt taataccaga
     2881 ggtaaatgac tgactgcaag caatttttcc gtgtaatctt ccagtttttt ttgtaaaatt
     2941 gaatagaact ctatttccag agaattaaca tttgttttaa cttccaactc catcaccctt
     3001 ctctctcaaa atcaaaacga ccaccacggt accccttaaa attgtaaaaa aacactccag
     3061 tactccatca aaacggacta gaaaagagga atttttaata tgaaacaagg aattgcagac
     3121 ataaaaataa ttaaagaaat tcttgaaaaa tctacggcta atgcaatagc attcggaaca
     3181 ggaatcaact tgagtacagt aaaaaaactt aaaagtggag agagagctga agaaaaatta
     3241 aatcttgccg atgctattaa aatcacagaa tttggcatga aaaatatgcc aaccaaaatc
     3301 gaaatatgga aatcctagct cttccaaagc catttgtcgt tggtaccaaa cactgccgag
     3361 acttttccca ataatatcgc caatttcacg atcattaaga ccgatgaaga aggacataaa
     3421 aatgatttct tgtctttgac taggtaatgc tgcaatcgca tttgccaact cctcattgga
     3481 aataaagatt ttcataccaa ggacaataaa agtaccacta ctattcaaat ttggttccaa
     3541 ataatagaac tctgtaccta accctttttg ttccaatatt tccattgata tttgccgttg
     3601 gcgaaatcta gcgtacttct tttgaatatt taacgcctca ttgcagacta ctttcttaca
     3661 aaaactgtca aacgtatgtt gaatgtgggt ttcctctgag tcgcccataa tattctcccc
     3721 ctcaaaaaat tcattcaact cgttttactt ctatcccttt ccattattac tacaatctag
     3781 ga
//
LOCUS       DAJSBC010000142         3715 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSBC010000142 DAJSBC010000000
VERSION     DAJSBC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.145"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ONT65_002766"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ONT65_002766"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR2330339.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ONT65_002767"
     CDS             complement(2262..2513)
                     /locus_tag="ONT65_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR2330340.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ONT65_002768"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ONT65_002768"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR2330341.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ONT65_002769"
     CDS             3463..3681
                     /locus_tag="ONT65_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330342.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1026 a          797 c          688 g         1204 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caaga
//
LOCUS       DAJSBC010000143         3693 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSBC010000143 DAJSBC010000000
VERSION     DAJSBC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.146"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..235
                     /locus_tag="ONT65_002770"
     CDS             <1..235
                     /locus_tag="ONT65_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2330343.1"
                     /translation="SGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVI
                     TNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            470..706
                     /locus_tag="ONT65_002771"
     CDS             470..706
                     /locus_tag="ONT65_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330344.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            713..2665
                     /locus_tag="ONT65_002772"
     CDS             713..2665
                     /locus_tag="ONT65_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2330345.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT65_002773"
     CDS             complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT65_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2330346.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3464..>3693
                     /locus_tag="ONT65_002774"
     CDS             3464..>3693
                     /locus_tag="ONT65_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2330347.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSV"
BASE COUNT         1313 a          590 c          642 g         1148 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
       61 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
      121 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
      181 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
      241 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
      301 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
      361 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
      421 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
      481 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
      541 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
      601 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
      661 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
      721 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
      781 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
      841 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
      901 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
      961 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
     1021 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
     1081 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
     1141 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
     1201 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
     1261 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
     1321 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
     1381 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
     1441 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
     1501 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
     1561 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
     1621 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
     1681 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
     1741 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
     1801 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
     1861 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
     1921 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
     1981 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
     2041 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
     2101 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
     2161 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
     2221 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
     2281 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
     2341 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
     2401 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
     2461 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
     2521 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
     2581 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
     2641 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
     2701 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
     2761 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
     2821 tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg
     2881 atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc
     2941 tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga
     3001 atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct
     3061 tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg
     3121 tccctttgta gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt
     3181 ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa
     3241 cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc
     3301 attatcctgc ccgttagttg aataagcaat tttttctata agttatatta tcataatacc
     3361 acaaaatcac aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt
     3421 atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc
     3481 attttgtact ccaaaactga actacttact tgcatatata cgcctttata gtggagtact
     3541 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
     3601 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
     3661 tttgcaaaat gagtttttga agttaaatag tgt
//
LOCUS       DAJSBC010000144         3432 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSBC010000144 DAJSBC010000000
VERSION     DAJSBC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.149"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..830
                     /locus_tag="ONT65_002775"
     CDS             12..830
                     /locus_tag="ONT65_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2330348.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            complement(1513..1611)
                     /locus_tag="ONT65_002776"
                     /pseudo
     CDS             complement(1513..1611)
                     /locus_tag="ONT65_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1876..2331)
                     /gene="tnpA"
                     /locus_tag="ONT65_002777"
     CDS             complement(1876..2331)
                     /gene="tnpA"
                     /locus_tag="ONT65_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR2330349.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2574..3432
                     /locus_tag="ONT65_002778"
                     /pseudo
     CDS             2574..3432
                     /locus_tag="ONT65_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1137 a          586 c          598 g         1111 t
ORIGIN      
        1 gaggagtttg tatgagttat atctatttaa ttaaagaaaa ttatccattt ttaacgaagt
       61 ctgaaaaaaa agtcgctgat tttattttaa attctgggaa aactattatt tatagtacga
      121 tgagtgatat taagaaaaac acaaaagttg gcgatgctac tattattcgc ttttgtcaaa
      181 aaattggttt ttctggtttt tctgatttaa aaattgaaat tgcaaaggaa gactttagtc
      241 aaaaaaaaga acatccgact agtgtaaagt actatgatga aattgctaat agtttaacag
      301 aagctctcta ttccactatt aggcttttag acgaagaaaa attgaatgaa gccattaaaa
      361 tgatcactca atcaaaaaac atatatatat ttggagtggg atctagtgga aatactagtc
      421 ttgatttaga aaatatgttt cttcgcgtag gagttcaagc aaaagctgta ctagaccctc
      481 attttcaagc ccaagtcgct tctttattaa cagttaatga tttagtaatc attttttctt
      541 tatctgggaa aacgaaagat acttatgatt cattgaaaat agctaaaaaa aatggcgcaa
      601 aaattattgc tataactaac tatattcatt cacctatagg aaagtctgca gacttagttt
      661 tacaaacagc cattgaagaa tttcttaatg gtggatcttt agctggaaaa atatcacaac
      721 tttatatttg tgatctatta gttcatggtt atgaacaaaa caataatatt gattcagttg
      781 aattaagaga aaaagttttg cgctctatta ttgataaaag tattgagtga actaccccca
      841 cttagctaaa cctaacggtt tttaacgatt gaagtaggca attcttggga tgagagcata
      901 cttgatagct tattttttac taatagcggt ggctcttatt gaccaaatat ccctgtagtt
      961 cctacggttc ttgattttat ctaaagatat ttttatcgtg gaagctattg atcaacttga
     1021 aagaaaaacg tgtagctcat tatcactagt ttatcgagta tggatgaagc aatggatcat
     1081 cctctcttaa ataaatttat taaggatctg atccttcaaa ttttttccat gatcgatgag
     1141 caggaaccta ctgaatctaa acgacatcgg gcacaaggaa ttattaagat tgctaagcga
     1201 atgttttcta taaagggtgt tctaagctat acagtgttaa ttcaaaagat tcccaatcat
     1261 cttgtttata agaacaacgt tgaagattaa aaaagtgttt caatgctaaa attgcaaaat
     1321 acttcaatgt tatgagacaa actgtctata agaatcaatt tccacccatt tcatcacttg
     1381 ttaaaagttt atgatccttt tgatttttat attaatttat ctgtatttaa tacagatttt
     1441 atttattagt tcatctgcct attgtatttt attttgttgc cttaccatct gaagtaaata
     1501 tactcttaat tgtcaaatta agttagacag atcatcaata cttacgtaac tcaaaaatac
     1561 ttccaaaggt gttcgataat ccagtgattt tctaggaaga ttatttctgc gccagcattt
     1621 tctaatgttt gaatttgtgt tttgtaatct tggttgcttg tgctaattct tgaatagccg
     1681 taaattgcca aaatttcatc accctttctg gacataagtt tctgacacca gttaaaatca
     1741 agtctatcag taacccatac acccgtgagg acgggtgtat ttttaatccc cctagaaggg
     1801 gtcgacactc gctgcccctt aaaaggggat ttttaaaaga ccgccaactg ctcatcttca
     1861 ctcacttact tctctttatt ttttaaatgg ttctacgtac tctcttttac ttataatata
     1921 tcgaatgcga tcatcagctt cttgttcacg gatgtatttt tcatttgtct tttgattcaa
     1981 tccgactgta ctcacatagt atctcttcga ccaaaaggat ttgtttccgt atttatattt
     2041 gagatttgcg tgctgctcgt ggattagtac cgcactacga cctttcagat accccataaa
     2101 actggatata catatttttg gtggtactcg aacaagcatg tgaatatgat ctagcattgc
     2161 atgtgcttct atgatttcca catctttcat ttcgcacaac ttcctaaata tttttccaag
     2221 atcttttcgt aattgcccat agattacttt tcgttgatat tttggtgtaa aaataatatg
     2281 atacttacaa ttccaacgag tatatgctaa acttttatcg tcatttgaca tgaaaaaact
     2341 cctttcgatc tgatgagcgg ttggtagcca ctcactagta tatcgaaagg agtttttatc
     2401 gctaaagata cttcttccac gcgtagaaac gcgcggtttt cttgtttcgc acgctttacg
     2461 cacaaaactg actgaagtca ttaagaaaat aaaagggtct aaaactttta agttttgtac
     2521 cgacccccaa aagttagacc aaaaaatcta acttttggag gtcggtgttt tttatggcta
     2581 agtatacttt tgaacttaaa ttaaagatcg ttcatgatta cttggatggt aaaggcggaa
     2641 gtgattatct ggcaaagaaa tattccatta aggctccatc acaagtgaag cgctggatca
     2701 atgcctatca agaatttggt gaagagggtc tagtaagaaa gcgacaaaag aaaaaatatt
     2761 ctgttcaatt taagctggat gcgatagagt tatatctaac aagtgagctg tcctatcgtg
     2821 aagttgccaa cacacttaac atgaacaacc ctaatttgat cgctagttgg atgcgtcaat
     2881 tccgtgaggg tgggatcgat ggactctcaa aaacgaaagg atgtccacct atcttgtcta
     2941 agaaaaatga acccaaaaat aagaaaaaat ctacaactaa agcaacgtct aaagaacgtg
     3001 aacgcatcga ggagttggag aaacgagtcc gttcccttca aattgaaaat gcctttttaa
     3061 aagaattgag gaagctgcga aaacaggaag cccaacaacg acgaatgaag caatcgcacg
     3121 aatcatcgca agcctccgag gaccgtttaa attagttgag cttctagaaa ctttaaagtt
     3181 ccctaaggca atctttatgt actggcaaaa acgattggac cgtaaaaatc cagatcaaga
     3241 gattgaagcg gaaatgctta aagttcggga gaagcacaag gattatggct gtttacggat
     3301 gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa aagaaagttc agcgcctgat
     3361 caagaagcta ggaataaaag taacgacata cacacgtaaa tcacggcgct acaattctta
     3421 tcgaggacaa gt
//
LOCUS       DAJSBC010000145         3424 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSBC010000145 DAJSBC010000000
VERSION     DAJSBC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.150"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            286..1236
                     /locus_tag="ONT65_002779"
     CDS             286..1236
                     /locus_tag="ONT65_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2330350.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1257..1790
                     /locus_tag="ONT65_002780"
     CDS             1257..1790
                     /locus_tag="ONT65_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2330351.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1806..1976
                     /locus_tag="ONT65_002781"
     CDS             1806..1976
                     /locus_tag="ONT65_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330352.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            2426..2959
                     /locus_tag="ONT65_002782"
     CDS             2426..2959
                     /locus_tag="ONT65_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330353.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2971..3174
                     /locus_tag="ONT65_002783"
     CDS             2971..3174
                     /locus_tag="ONT65_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330354.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
BASE COUNT         1265 a          546 c          604 g         1009 t
ORIGIN      
        1 tttgtcaccc tcttttataa tctcttttta aacctttttt taaacctctt taccgtcggc
       61 tgtgtcctta ctccttcaat ggatacagcg ttttataaac tacattttgt ctgcttttaa
      121 actacatttt gtctgctttt aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
      181 ttttaaactc tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt
      241 tgtttataac gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc
      301 gtgaaatacc atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg
      361 aatctatttt tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt
      421 acttttgacc aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt
      481 aatgatttag aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct
      541 aatgggctcg atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct
      601 aaagatccat atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta
      661 gatgagtggg ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag
      721 actatgtttc gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag
      781 gattttcatg aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat
      841 aatcgtgtta taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc
      901 aaaaagaaat acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa
      961 ccagaaatca aaaaagcaga tgattttaat aaacatgcac caaccaaaga agaacaaaat
     1021 aaagcattgg ctttagctga taaacaaaaa gaatacgaag aacctgctac aacaattaac
     1081 gaaaaaatca aacagaagaa acgagaactt gaactcctcc aacaaatcaa acaactagaa
     1141 aaagagaaag aagctcttga aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa
     1201 agtttattat ctgatttaga taatttattc cagtagcaac ctaggaggca accagtatgc
     1261 aaccacaaga aacctttaca ataaaggaat tgtcagaact ttttaagatg agtaggcaag
     1321 caatatcgaa acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt
     1381 ataaggttgt attgcgttct ggagtattgc aacttgctcg caacttaggc aaccaaatgt
     1441 tgctagatag gttgcacgag tacgaacaag acaacgataa aaatgttaat ataccaagca
     1501 aaaacgtggt tacaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag
     1561 aacaacttgc cctcagagag caaaataatt ctcatataca aaaactactc gaccagcaac
     1621 aacaactcac cctacaagcc aacaaacaaa tcgaaaaact gcaagaaaaa ctacagttga
     1681 cttatacaga agaagctcca gagaatgact caacgactct ttctgaagca acagaaacaa
     1741 tagacaaaca aattgatact gaaaaaaaat ggtggcaact ttggaaataa ctggagggaa
     1801 cattcatgac aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggattca
     1861 gtccgatgtt attgtcagtg atcaataaag ggaataaaca tggtgaattg aaattgaatc
     1921 caaattatta caacagtcct aacgaagaat tgtttgtaaa agaaacagat aaataataaa
     1981 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     2041 acgcgcttat gaagcgctac caaaaattta tcatatttta ttaaaaataa caacccagta
     2101 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caaccgcaga aaatcaagct
     2161 taaaaagagc ttttgaccta gaccaatcga aaatcacaaa gtgagcgcct gtatctattg
     2221 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
     2281 tcctaatgca ttctaactat accacattat caaaaatata aattatcttt actaaataaa
     2341 atattgacaa gcctatacct tttgttttat gattttcaca aagaaaacgt tttctatata
     2401 tttttctgta tcaggaggaa attctatgaa aaaatttact ttaggggtag ctttgttaac
     2461 tattggggca gttgttttac cgtcaactac tatagtttat gcttcagaaa attcccaagt
     2521 ggtatcatta aacgaaaaca atgctactga aaatgtagtt attgagtaca ctgaagatgg
     2581 tatggtcgta actaaatatg aggatggtta tagtgcggaa gattttgcaa attcttcaga
     2641 tatcatgaca agagctgcag ttgcaagatg gggcaaaaag gaatatacaa atattgctgt
     2701 aaccactgga gtagctgcta atgctattaa tgctgccttt tgggcaggag ggaaagctag
     2761 cacttctatg tttggtatcc caggttgggc aattggtgct ttgttaacag gtgcaagttg
     2821 gactaaaaaa ggtgcttctc cagggaatgc agttgcaaaa gaatgggaca aaaatggtaa
     2881 tggctggatt ggattttatt accaaagggg atatgatgca gctggaagag ttgtagctac
     2941 tagatattca acgttataag ttaaggagtg atgtttttga tatctaaatc attaaaatca
     3001 ttaaaaacat ttactaaaaa gaatccgcaa atagctacta ttcgtatttt tatatttgct
     3061 atagctgttg ctataattgt taatatgcta agcggagggc aattatacaa atctggttta
     3121 tttttagtgt taatcttagt tgtcttagac ataataaagt ctaaaaaaaa ataaatcgat
     3181 acattttaga agaatagggt tgtttgaata ttacaaagca acaatttcac cttcggtgaa
     3241 ataagcgaaa ctttacaaaa agtggctagc aaatgctagc ctttttttgt attgtggagc
     3301 gtggattttg agggggattc aagggggagc ttgcgctcct ttgacaagcg gttatttgca
     3361 ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt
     3421 tact
//
LOCUS       DAJSBC010000146         3401 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSBC010000146 DAJSBC010000000
VERSION     DAJSBC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.151"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            150..1190
                     /locus_tag="ONT65_002784"
     CDS             150..1190
                     /locus_tag="ONT65_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2330355.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2014..2346
                     /locus_tag="ONT65_002785"
     CDS             2014..2346
                     /locus_tag="ONT65_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2330356.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2965..3306
                     /locus_tag="ONT65_002786"
     CDS             2965..3306
                     /locus_tag="ONT65_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2330357.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1265 a          460 c          644 g         1032 t
ORIGIN      
        1 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
       61 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      121 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata
      181 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      241 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
      301 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
      361 aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
      421 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
      481 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
      541 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
      601 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
      661 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
      721 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
      781 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttgtccctg
      841 aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg
      901 tccagactat tcataatgca aagaaaaaag ctgaaataga aagtggtatt tcgatagttt
      961 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1021 aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct
     1081 atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt
     1141 tgaattatga aagctcccca agcactatta cgaaagactg gttagagtga aactttactg
     1201 acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattctaat
     1261 tatattacga atattacaaa ataatagcta ttttaataga atttataagt taaaatatta
     1321 tgataaaaat cacaaacgta gccttttacg tcataagtgt taaaagaaat aattaatttc
     1381 aaatatagaa aagggaacgt atgtttgcat actccttttt tttgagttat aataatacgc
     1441 gaagagagat aatttcatta tttaaacaag ctaaaaatct tgatattact tcttttcagg
     1501 caatacgaat attattcgct tacatttttt gtataaattt aattgtctgc tatgcttgtg
     1561 atgaattgat tttcgttctg cacttttgga tgataaattt ggacaattga aaatacaaaa
     1621 aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc atacacggca
     1681 ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc gcaatggatt
     1741 tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag gttaaaaata
     1801 cgtgatatta cgatgaatct atgcctaagc acaatgttat aaaaatgtcc gtgtggctct
     1861 ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt tccctctttt
     1921 ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga aaaacttgtt
     1981 aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat tttgagtctc
     2041 aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac gaagaaacta
     2101 tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca gaaagacaaa
     2161 gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt aaaagatcct
     2221 atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa cggtgtactg
     2281 attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc
     2341 ggctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct tgaaaatatt
     2401 gtcgagactt ctaaaggaac ttagagtcac tagaaaaata aggagtagac ttgatctatc
     2461 aacaattccc ttcggggagt gacggggtag gttttaacga ttggtaacgt aaaaaattgg
     2521 tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta aataataagc
     2581 gcagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgtc tagattaatc
     2641 atgagcagct actaagggat ctagactttg acgcaaactc atggaataag gagtaggtct
     2701 tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact
     2761 tgcgaggacg ttgattattg gatcaatcga gcatttaccg ctgtctacta gggtttctcg
     2821 tttcggacgc cataaacgag tcgaaatttt ttatgtattt ttaagtaaac gaaaaacgat
     2881 aaaatatgaa caagtcctga ataaagtgaa aaaattgata taaaacatag agaaaatgaa
     2941 atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg attgggcaca
     3001 catactatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac gtttagaaga
     3061 ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt atacgatcaa
     3121 acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga aggtaatgaa
     3181 tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga agatacttgg
     3241 tcatatttga gtgattatat tgtcagcttt gaaatcattg aatttgtagc ggataaagaa
     3301 gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc acgatgatta
     3361 ataaaaaggt agtattacct taggcattga gtaaggaatt t
//
LOCUS       DAJSBC010000147         3391 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSBC010000147 DAJSBC010000000
VERSION     DAJSBC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.152"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..2493)
                     /locus_tag="ONT65_002787"
     CDS             complement(22..2493)
                     /locus_tag="ONT65_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2330358.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="ONT65_002788"
     CDS             2766..>3391
                     /locus_tag="ONT65_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330359.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DAJSBC010000148         3362 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSBC010000148 DAJSBC010000000
VERSION     DAJSBC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.153"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2232)
                     /locus_tag="ONT65_002789"
     CDS             complement(<1..2232)
                     /locus_tag="ONT65_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330360.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ONT65_002790"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ONT65_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          927 a          749 c          540 g         1146 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gc
//
LOCUS       DAJSBC010000149         3318 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSBC010000149 DAJSBC010000000
VERSION     DAJSBC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.154"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            135..347
                     /locus_tag="ONT65_002791"
     CDS             135..347
                     /locus_tag="ONT65_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330361.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(625..2424)
                     /locus_tag="ONT65_002792"
     CDS             complement(625..2424)
                     /locus_tag="ONT65_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2330362.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2548..3144)
                     /locus_tag="ONT65_002793"
     CDS             complement(2548..3144)
                     /locus_tag="ONT65_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2330363.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1205 a          597 c          584 g          932 t
ORIGIN      
        1 tagttatcta aaaagcagaa agatactcaa aataaaaaat aaaaaaatgg ataaaaacgt
       61 tttcaaatac aaggtaattt gttattataa aataaagaaa aaacaaaata aaaataaaga
      121 aaagagggaa tgaaatggta aaagcagaag taggaagatc ttatatatgc aaaccgatcg
      181 gagtatctca tgaactaatc ggctgtattg aacgtacata tatcaacacg gctgtcgtgt
      241 atgttgaaag ttatgaccaa cgagactatc aacttatcaa tgaaagcaag tatcgcatgc
      301 tcgtaaaatt ctgtgatatt agcgcacctg ctgaattggt ttcttaaaca gtatttttga
      361 gaattcgcaa gaactccctt ataatataaa taaagacaga agggagcgag aagatatgcc
      421 aaaaagaaaa agttcaggga aaatgggaat tattcttgca gtgatctctg caattcctgc
      481 tattttagct attgttcgtg cgatgaagga atcaagagaa agttaagcac acattatgag
      541 cgtcaaacaa aaaacgcata tacctgcggt ggcaggttat gcgttttttg ttgtataaag
      601 acttttttca ttaacgattt ttgattagcc gactcccaaa acaaaattta gcagtttatc
      661 tgaataagtg atttcttcat ctgtttgttt cacactttca tttagtagtc ccatctcatc
      721 ggttatgata ccatccacac cataaaacat catgcgactc atagcatctg aatcgttgat
      781 tgtccataca tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat
      841 cgtactatat tccattgtca gaaaatcagc aggcgtcaca gggggcccaa caatattgaa
      901 aggtaagatg tagcccacat aaaagtttgg ttctgcttct tttaaatctg aaacggtttg
      961 gtacgtcaaa ctttgcaaaa tatgatggtt ctctaagata actttgcggt atttatggac
     1021 aaatcgttcg acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa taagtaattt
     1081 ttggtttagc ttatgtgctt tttccaaata agaatcaaaa gaacaaacag gggcttgcac
     1141 tccgttttca gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc
     1201 atttacccct gttaagtgac gaagatttgc atcatgcatc actacgaaat gaccatcttt
     1261 tgtttcctga acatccatct ctacataatc aggatgatag tctctgcttg ttttttcaag
     1321 tgcttctaaa gtattttgcg ccccattttg ggtactcaca cctctgtgag agacagtcac
     1381 tggttttgtc tgaacagctg aagtcagata atccatattg taaagacaga cgccaatacc
     1441 gaaaaaaact gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac gcaagttggg
     1501 ggcttgtggg ataaaccact tagggatctc aggcaagaag ccttcgtcat ccatgaaatc
     1561 aacaatgata taaaaaatcc ctacggtaga catcacgata ttgaataata aaatcccttg
     1621 taaaagcgtc atagcaaaca cagcgctgat caatgcatag tctgggaaaa attgttcaac
     1681 catactttgt gccaaaataa caacgatata tcctaaagaa gaaaggagta aaatcgtacc
     1741 gccaatcaca ataaattggc ccgtgatggc caaaagacgc gatttggtca atgaccagct
     1801 ttcccgaatg gctgctcgaa aaggacgatc tcgtagaatc atttccggta aagcaaaaat
     1861 cagtcgaatc ccaatatagc caaggaaaat ataaacgagt aaaaaggaac tgacgatgat
     1921 ccaacgatta gcaaaaataa aatccataat gaaagcgggg atttttattt ttgacagtag
     1981 atcagaatta aaacttaatc cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa
     2041 caaaaaagta atgggtctca ctttcttcag ctgaagtatc gtcaagtgta agagttgctt
     2101 caaagagatt ggttctttct ttttaataaa aaaaacactc atcaaaagaa aagtaaattc
     2161 gaaataaact aacagtaaaa tcgtcatcaa aactacgatt aacgagagta gaaccccagg
     2221 atgttgagaa aaaattgaag gaatattgtc ataagacaaa tagtcaattg cccctcggtg
     2281 taaaataaaa cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa taaagccatg
     2341 catcagcaag acatcacgga aataagcttg tgttcctttt aaaaagtccc aagcattacc
     2401 taagctacgt ttcaaataat tcatcctatc acctgctctt cacattgtag cgcaaatcta
     2461 atgaaaaagg aaagggtgca gtgtgttttg cagacccttt cccagattta acattgtcaa
     2521 tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat attgacctcc
     2581 ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca aaatgattag
     2641 tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg catcgtcagg
     2701 agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt gattatccag
     2761 ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga atggctttcc
     2821 ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa tatagttata
     2881 gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga tccctccagg
     2941 aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg gtcctaaaat
     3001 gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac ctaaattaat
     3061 aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga aatttatcag
     3121 ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt tgttaactat
     3181 actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa caaacaattt
     3241 tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact aaagacttat
     3301 ttaataactt gttttttt
//
LOCUS       DAJSBC010000150         3295 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSBC010000150 DAJSBC010000000
VERSION     DAJSBC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.155"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(379..666)
                     /locus_tag="ONT65_002794"
     CDS             complement(379..666)
                     /locus_tag="ONT65_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2330364.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(686..910)
                     /locus_tag="ONT65_002795"
     CDS             complement(686..910)
                     /locus_tag="ONT65_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR2330365.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1365..1535)
                     /locus_tag="ONT65_002796"
     CDS             complement(1365..1535)
                     /locus_tag="ONT65_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330366.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1550..2083)
                     /locus_tag="ONT65_002797"
     CDS             complement(1550..2083)
                     /locus_tag="ONT65_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2330367.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2104..3048)
                     /locus_tag="ONT65_002798"
     CDS             complement(2104..3048)
                     /locus_tag="ONT65_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2330368.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT          932 a          582 c          498 g         1283 t
ORIGIN      
        1 agtaaagctc actaaagctc actaaagggc ttggcaagct ctatatttgc tagccaaata
       61 cctacgtatt tggtgcaaat aaccgcttgt caaaggggcg caagctcccc cttgaatccc
      121 cctccccccc actctccaca atacaaaaaa gactagcaaa tgctagccac tttttgtaaa
      181 gtttcgcttg tttcaccttc ggtgaaattg ttgcaaatca ataattaggt agagctacat
      241 ttttttcgtt gttcaaagat tcttatgcca tttactatta ataaatctat aaatatgata
      301 atttttcggc tctataattt atgggactga tgaatcagaa ttgattcttc agtcccattt
      361 tcattttatt ccaaagaatt aactccaaat accaatagaa gcccatcctc tatatacttg
      421 agattcctta ctaacaattt cttgtaattc tattaaagat ttaggtaatc caaatttatg
      481 ttttaaaata tatcttccaa gaaaactact taatttcata ctaacttcac caacactttt
      541 tccctcactg atttctttag aataaagaaa gagcttttct tttaagtcag gatagtcatc
      601 tatagttgct tcattgtata ctttactaat tgcatttaaa agtttttctt ctttagtaaa
      661 atccattata ataacctccc taccactacc ttctaggtgc ccatggacca tgattaaccc
      721 aaccattaac aataattttt ccaatttctc ctttagcttg attccaatct acccaacatt
      781 tttctttgtt acaataaaga ccatttccat agtaagtagc tgcatcaact tttattcctg
      841 taccgatgcc agctagacaa gttcctaata ttcctagaat aacacaatgt tttaatactt
      901 tctttttcat gattgttttt ccttttcgtt tttttaaaat tgcttgttat attaaataat
      961 cttagtaatt ttattatata acattttatt atataacaat gattaacgtc ataaaaagta
     1021 gttttgaatt cataatcgtt taaataaaat tataagatca gttatttaaa cagctagtag
     1081 acataatgtg tatactacca agtaacgcct gtaccaaggg atccaggcgc tgattttgtg
     1141 aatttttatt ggtctaggtt aaaagctctt tttaaggctt ggttttctgc ggttgtttct
     1201 ttctaattct ttgtgaatat tgatcggtca ttttttaaat acttgttatt tttgtttgtg
     1261 tttgttatgc tttttacata tctcatattc atttattatt cttttctaag gttaataata
     1321 ggataatctc tttcagaaag actgctcgta cgagcagtct tttcttattt atcaagtttt
     1381 tctacaaata cttcctcatt ttgactgttg tagtaattag gattgagttt tagttcccct
     1441 tttttatttc ctttgttggc cactgataat aacattgggc taaagcttaa tattcctagt
     1501 aggatagaac cgaatagcaa gtgttttttt gtcataagta tttccttctt catttccata
     1561 gctgccacca ttttttccaa ttctttgttt gttcgtccat tgtttctgtt gcttcagaaa
     1621 gagtcgttga actattctct gaagactctt ccgtataagt caactgcagt tgctcctgaa
     1681 gtttttcgat ttgttggttg gcttgcaggg tgagttgttg ttgctggtcg agtagttttt
     1741 gtatatgaga attattttgc tctttgaggg caagttgttc tcgtaaaaat tcattttctt
     1801 cacgcaactg atctagcaac ttgtttgcaa ccacgttttt gtttggtata taagcatttt
     1861 tatcgttgtt ttgttcatgt ttatgcaact tatctagcaa cctttgattg tctaagttgc
     1921 gagcaagttg caatactcca gaacgcaata caaccttata accacgctca tttttagcta
     1981 tcatggaaga atctaatttt tggatatgtt tcgatattgc ttgcctactc atcttaaaaa
     2041 gatctgacaa ttcctttatt gtaaaggttt cttgtggttg catactggtt gcctcctagg
     2101 ttgctactgg aataaattat ctaaatcaga taataaactt tttttctctt tttccgaaag
     2161 tgcggactct gtttgttttt caagagcttt tttctctttt tctagttgtt tgatttgttg
     2221 gaggagttca agttctcgtt tcttctgttt gattttttcg ttaattgttg tagcgggttc
     2281 ttcgtattct ttttgtttat cagctaaagc caatgcttta ttttggtctt ctttggttgg
     2341 tgcatgtttg ttaaagtcgt ctgctttttt gatctcaggt ttgaacgtaa attgatagcc
     2401 aataacaggt tttccccgtc cttttccgta tttctttttg attgttagtc ctttgaagat
     2461 tgcagttagt tcttgacgta taggttttat aactcgttta tctatatcag gttgcttata
     2521 acttttagga atatctaata attcaagaaa gtcttctttg gagaaatatg cgaaaccttt
     2581 ggttctgtat tgctttaaca gacgaaacat agtctttgca tagccacttt gtagctcgtt
     2641 gaattgttgt aaagaatagc gtacccactc gtctaaatta tttaacaaag gcagtgcttt
     2701 ttcgtaaatt cgtatatcta cgtaaggatt ttctacgtcc ccgttgattt taaattctgt
     2761 aaacattaca aacatatcgt aagaaagccc agactcactt ctacgaccaa agcgtaaaga
     2821 taagattttt tcataagttg cttttaaatc ttctacaaat cgtacatttg cagttgcttt
     2881 gtagttactt aaatctttta gttggtcaaa cgtaaaacga actgtcttat cccctttttc
     2941 acgcattcta gacacgatgg aaaaaaacaa attcatttca accggtgtaa attttcttaa
     3001 aggaattgta tttaattcat ggtggtattt tacaatctca ttagccatat aatcacctca
     3061 atccaattat gaatgacatt ttttaaatgt caagacattc atatttgacg ttcaaacaga
     3121 caaaatgtcc ttcataaaca gacaaaatgt ccttcataaa cagacaaaat gtccttcata
     3181 aacagacaaa atgtccttca taacattctg tatccattgg tagagtaagg acacagctta
     3241 cggtaaagag gtttaaaaag aggtttaaaa agagatataa aaagaggggg gctaa
//
LOCUS       DAJSBC010000151         3202 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSBC010000151 DAJSBC010000000
VERSION     DAJSBC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.156"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..3161
                     /locus_tag="ONT65_002799"
     CDS             12..3161
                     /locus_tag="ONT65_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR2330369.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1127 a          543 c          688 g          844 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagttga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcacgtacga cccatgatca gtatggagag gtcttgcaca
     1441 cctataccat caagaatgca ttagaagatg gctcggtttt aggatttcaa gtagagcatg
     1501 aaaacaccgt ggagccgact tcattagaga ataaaatcta tcggaaatta aaagaagtag
     1561 aaacatatgc agagtattca ccggaacaga tcaatcgtat gatcgatcaa atggagtctg
     1621 tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat atccaaaaag
     1681 tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt cgaaatggcc
     1741 gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa aagtattatc
     1801 gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt cccaatcagc
     1861 ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct attacctatt
     1921 cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa aaaattattg
     1981 aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag cgttataacg
     2041 gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa
     2101 tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct acgattcaaa
     2161 ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt tcacgtacga
     2221 accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag ccagcgacaa
     2281 tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa gaatcgggtt
     2341 tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat ggtaagttaa
     2401 aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt
     2461 acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact tatgatgatt
     2521 acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt ttggaagaac
     2581 aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca gaaaaagagg
     2641 gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg
     2701 attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag
     2761 aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc
     2821 gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca
     2881 ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt tccaatgagt
     2941 ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca ggtgaagatc
     3001 caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac
     3061 atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg cgtaaaatgt
     3121 tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga gttaagaaaa
     3181 ttgtacgatt caaagatagt ag
//
LOCUS       DAJSBC010000152         3129 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSBC010000152 DAJSBC010000000
VERSION     DAJSBC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.157"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            103..1347
                     /locus_tag="ONT65_002800"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ONT65_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ONT65_002801"
     CDS             1360..1992
                     /locus_tag="ONT65_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2330370.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="ONT65_002802"
     CDS             2197..2424
                     /locus_tag="ONT65_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR2330371.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="ONT65_002803"
     CDS             2493..2759
                     /locus_tag="ONT65_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2330372.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ONT65_002804"
     CDS             2749..3018
                     /locus_tag="ONT65_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2330373.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DAJSBC010000153         3114 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSBC010000153 DAJSBC010000000
VERSION     DAJSBC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.158"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..940)
                     /locus_tag="ONT65_002805"
                     /pseudo
     CDS             complement(1..940)
                     /locus_tag="ONT65_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(1161..1523)
                     /locus_tag="ONT65_002806"
     CDS             complement(1161..1523)
                     /locus_tag="ONT65_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330374.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1527..1865)
                     /locus_tag="ONT65_002807"
     CDS             complement(1527..1865)
                     /locus_tag="ONT65_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330375.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1844..2263)
                     /locus_tag="ONT65_002808"
     CDS             complement(1844..2263)
                     /locus_tag="ONT65_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330376.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2256..2489)
                     /locus_tag="ONT65_002809"
     CDS             complement(2256..2489)
                     /locus_tag="ONT65_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330377.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(2486..>3114)
                     /locus_tag="ONT65_002810"
     CDS             complement(2486..>3114)
                     /locus_tag="ONT65_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2330378.1"
                     /translation="SQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          868 a          701 c          433 g         1112 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
      121 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
      181 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
      241 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
      301 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
      361 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
      421 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
      481 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
      541 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
      601 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
      661 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
      721 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
      781 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
      841 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
      901 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
      961 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     1021 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     1081 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     1141 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     1201 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     1261 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     1321 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     1381 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     1441 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     1501 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     1561 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     1621 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     1681 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     1741 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     1801 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     1861 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     1921 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     1981 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     2041 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     2101 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     2161 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     2221 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     2281 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     2341 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     2401 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     2461 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     2521 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     2581 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     2641 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     2701 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     2761 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     2821 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     2881 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     2941 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     3001 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     3061 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaa
//
LOCUS       DAJSBC010000154         3087 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSBC010000154 DAJSBC010000000
VERSION     DAJSBC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.159"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT65_002811"
     CDS             complement(25..162)
                     /locus_tag="ONT65_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2330379.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT65_002812"
     CDS             complement(159..1343)
                     /locus_tag="ONT65_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2330380.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="ONT65_002813"
     CDS             complement(1602..1892)
                     /locus_tag="ONT65_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330381.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT65_002814"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT65_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          842 a          739 c          540 g          966 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattat
//
LOCUS       DAJSBC010000155         2948 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSBC010000155 DAJSBC010000000
VERSION     DAJSBC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.160"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1350
                     /locus_tag="ONT65_002815"
     CDS             <1..1350
                     /locus_tag="ONT65_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2330382.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="ONT65_002816"
     CDS             1484..2827
                     /locus_tag="ONT65_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2330383.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1171 a          398 c          561 g          818 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaat
//
LOCUS       DAJSBC010000156         2947 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSBC010000156 DAJSBC010000000
VERSION     DAJSBC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.161"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(449..865)
                     /locus_tag="ONT65_002817"
     CDS             complement(449..865)
                     /locus_tag="ONT65_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330384.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="ONT65_002818"
     CDS             complement(867..1148)
                     /locus_tag="ONT65_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330385.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="ONT65_002819"
     CDS             1948..2922
                     /locus_tag="ONT65_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR2330386.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAJSBC010000157         2905 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSBC010000157 DAJSBC010000000
VERSION     DAJSBC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.162"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1097)
                     /locus_tag="ONT65_002820"
     CDS             complement(<1..1097)
                     /locus_tag="ONT65_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2330387.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ONT65_002821"
     CDS             complement(1227..2603)
                     /locus_tag="ONT65_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2330388.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..>2905)
                     /locus_tag="ONT65_002822"
     CDS             complement(2603..>2905)
                     /locus_tag="ONT65_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2330389.1"
                     /translation="DKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDS
                     VTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT          881 a          557 c          408 g         1059 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatc
//
LOCUS       DAJSBC010000158         2830 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSBC010000158 DAJSBC010000000
VERSION     DAJSBC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.163"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            802..1056
                     /locus_tag="ONT65_002823"
                     /pseudo
     CDS             802..1056
                     /locus_tag="ONT65_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1126..1638
                     /locus_tag="ONT65_002824"
     CDS             1126..1638
                     /locus_tag="ONT65_002824"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330390.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="ONT65_002825"
     CDS             1635..2672
                     /locus_tag="ONT65_002825"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2330391.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          747 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac
//
LOCUS       DAJSBC010000159         2822 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSBC010000159 DAJSBC010000000
VERSION     DAJSBC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.164"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            108..797
                     /locus_tag="ONT65_002826"
     CDS             108..797
                     /locus_tag="ONT65_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2330392.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            811..2313
                     /locus_tag="ONT65_002827"
     CDS             811..2313
                     /locus_tag="ONT65_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2330393.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2326..2802
                     /locus_tag="ONT65_002828"
     CDS             2326..2802
                     /locus_tag="ONT65_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2330394.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT          910 a          384 c          561 g          967 t
ORIGIN      
        1 aaaactccta aatactccat ttttatcttt acttttatga aagcggatgc tatattgttt
       61 ttggagttgt tatccaaaaa taataaaaaa ttggagtgtt taggataatg gataaaaaga
      121 ggtttttaga tcttatttca aaaggtttga ttgtttcctg tcaagcatta cctggtgaac
      181 cattttacaa agaggaagaa agtttaatgc cttattttgc tttggctgct caaagggctg
      241 gagcagttgg tatacgtgca aattctgttc gagatattat tgaaattaga aagtgtgtat
      301 cattaccaat gattggaatt attaaaagag attatccacc agaatctcca ttcattactg
      361 ccactatgag agaagtagat gaattagtag aaaccggtgt tgaggtaatc gctcttgatt
      421 gcactcttag aaatcgacat gatggactat ctataaattc ttttattact aaaataaaag
      481 aaaaatatcc tcatcagtta tttatggcag atatttctac atttgaagaa ggcaaaaatg
      541 catataaagc aggaatagat tttgtaggaa ctactttaag tggatatact gaggaaagta
      601 aaaaacaaga tgggccagat atagatttga ttaaaaaatt ggtagaagaa gggatttgtg
      661 taattgctga gggaaggatt catactccag aacaagcaaa agagataaaa aaaataggag
      721 ttgcaggtat tgttgtgggt ggtgctatta ctcgaccaca ggaaattgca tcaagatttg
      781 ttaaagcatt aagttagggg gagaaataaa atgtttaaaa aattttcaca attaggacgc
      841 gctttcatgt taccaattgc tatattacca gtagccggat tactattggg actgggagga
      901 gcgttaacaa atgagagtgc tataaatgct tatccatttt tagatcaacc gtggttgcat
      961 acaattttaa gtattatgag ttatgctgga aatgcagttt ttgcaaacct agcactaatt
     1021 tttgcaattg gtattgctgt aggacttgca aatggagata aaggaacagc tggtttagct
     1081 ggtggagtag cttatctagt atatactgca actattagtg ggtttcttga acttttttca
     1141 gcaaaagatg caacactaga tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt
     1201 gcatttttac acaatcgtta tagaaaaatt gaattaccac aattcttagg tttttttgga
     1261 ggatcaagat ttattccgat tgtttctagt ttagctgcaa tcattattgg ttctttcttt
     1321 tatttaattt ggccaccaat tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa
     1381 atgggtagtt taggaacttt tttatatggt tttttattgc gtttaacagg tgctgttgga
     1441 ctacaccaca ccatttaccc tttgttctgg tatacatctt taggtggtac cgaaactgta
     1501 gctggatcaa caattgctgg agcccaaaat attttctttg cccaactagc tgatccaaac
     1561 cacactggtc tgtttactta tggtactaga ttttttgcag gaagatttgc aacaatgatg
     1621 tttggattac cagcagcatg ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa
     1681 aagaatggtg ggctttattt tagcggggcc cttacatcat ttttaactgg aattactgaa
     1741 ccagttgaat atatgttttt atttgttgct ccttggcttt atgtgattca tgcattctta
     1801 gatggattat ctttttattt tgctgatatc ttaaatattc gtgtagggaa ttccttttca
     1861 ggtggtttga tcgacttttt attattcggt gtacttcaag ggaatgacaa aactaattgg
     1921 ttaaaattaa ttccttttgg tattgcatgg gcatttatat atttctttgt ttttacattc
     1981 tttatcaaaa aatttaaggt agctattcct ggaatggaag aagaatcaga agttcattct
     2041 gaaaaagtaa taaacaaaaa tacatcatta catgaagagt ctcttatcat tattgaagct
     2101 cttggtggaa aagaaaatat tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct
     2161 gttacgaatg gggagattat tcaaaaacaa acaataaaag acactggtgc aactgctgtc
     2221 ttcgaagtga agggtggcat ccaagctgtt tttggaggga aagctgactt attaagtcaa
     2281 gaaatcaatc aaatattagg aaaagataat taggaggttt ttaatatgtt tgaaattttc
     2341 aaaaagaaaa aacaagaaac tttttatgca ccttgttccg gaaaaatcat ttccatcaca
     2401 gaagtaaatg atcctgtctt ttcacaaaaa ttactaggtg atggttttgc cgtcatacct
     2461 gatggttcaa aaatttatgc tccattggct ggtcgagtaa caaatatatt tcctactaaa
     2521 catgctatta atataaaaag caactcaggc attgattatt tgattcatat tggaattgat
     2581 acagttgaat tacaaggaga gcctttttcg atttgtgttc aagaaaatga tgaggttact
     2641 cctgaaacct cgttggttga tgtggatttt gacaaaataa taaaatctgg taaagatact
     2701 tctgttatag ttgtttttcc ggagaaaaat cagttcaatg atttaataat agataatggt
     2761 cgaattgctc atgggaattc atgtggaaaa atcacttttt aaaaaagtgt atataaaaag
     2821 ct
//
LOCUS       DAJSBC010000160         2820 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSBC010000160 DAJSBC010000000
VERSION     DAJSBC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.165"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..936)
                     /locus_tag="ONT65_002829"
     CDS             complement(61..936)
                     /locus_tag="ONT65_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2330395.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1191..1275)
                     /locus_tag="ONT65_002830"
     tRNA            complement(1191..1275)
                     /locus_tag="ONT65_002830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1239..1241),aa:Leu,seq:caa)
     gene            complement(1293..1364)
                     /locus_tag="ONT65_002831"
     tRNA            complement(1293..1364)
                     /locus_tag="ONT65_002831"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Gln,seq:ttg)
     gene            complement(1374..1446)
                     /locus_tag="ONT65_002832"
     tRNA            complement(1374..1446)
                     /locus_tag="ONT65_002832"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1411..1413),aa:His,seq:gtg)
     gene            complement(1508..1592)
                     /locus_tag="ONT65_002833"
     tRNA            complement(1508..1592)
                     /locus_tag="ONT65_002833"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            complement(1613..1683)
                     /locus_tag="ONT65_002834"
     tRNA            complement(1613..1683)
                     /locus_tag="ONT65_002834"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Cys,seq:gca)
     gene            complement(1729..1800)
                     /locus_tag="ONT65_002835"
     tRNA            complement(1729..1800)
                     /locus_tag="ONT65_002835"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1766..1768),aa:Gln,seq:ttg)
     gene            complement(1812..1884)
                     /locus_tag="ONT65_002836"
     tRNA            complement(1812..1884)
                     /locus_tag="ONT65_002836"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:His,seq:gtg)
     gene            complement(1887..1960)
                     /locus_tag="ONT65_002837"
     tRNA            complement(1887..1960)
                     /locus_tag="ONT65_002837"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1926..1928),aa:Trp,seq:cca)
     gene            complement(1967..2047)
                     /locus_tag="ONT65_002838"
     tRNA            complement(1967..2047)
                     /locus_tag="ONT65_002838"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2011..2013),aa:Tyr,seq:gta)
     gene            complement(2049..2124)
                     /locus_tag="ONT65_002839"
     tRNA            complement(2049..2124)
                     /locus_tag="ONT65_002839"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2089..2091),aa:Phe,seq:gaa)
     gene            complement(2160..2232)
                     /locus_tag="ONT65_002840"
     tRNA            complement(2160..2232)
                     /locus_tag="ONT65_002840"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2197..2199),aa:Asp,seq:gtc)
     gene            complement(2242..2314)
                     /locus_tag="ONT65_002841"
     tRNA            complement(2242..2314)
                     /locus_tag="ONT65_002841"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2279..2281),aa:Val,seq:tac)
     gene            complement(2330..2401)
                     /locus_tag="ONT65_002842"
     tRNA            complement(2330..2401)
                     /locus_tag="ONT65_002842"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2366..2368),aa:Glu,seq:ttc)
     gene            complement(2416..2505)
                     /locus_tag="ONT65_002843"
     tRNA            complement(2416..2505)
                     /locus_tag="ONT65_002843"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2469..2471),aa:Ser,seq:gga)
     gene            complement(2545..2618)
                     /locus_tag="ONT65_002844"
     tRNA            complement(2545..2618)
                     /locus_tag="ONT65_002844"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2582..2584),aa:Asn,seq:gtt)
     gene            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT65_002845"
     rRNA            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT65_002845"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          829 a          652 c          536 g          803 t
ORIGIN      
        1 aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta cgcccttatt
       61 ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt gaatggcaag
      121 ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg ctttgctatg
      181 tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct gataaatcaa
      241 gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg attctttatc
      301 ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca tttctactat
      361 ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat gcatagtgat
      421 gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc gctcgaccac
      481 ttctactgct atatcatatt catttggata aaggaatctc atttctggca acataattgc
      541 tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat ctgtcaatgt
      601 aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt caatcacttc
      661 ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga atcgtttgat
      721 cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa atggcttact
      781 cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc catcgtcatc
      841 gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt cacctcctct
      901 atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct ccaaacaatt
      961 tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct aattgaatag
     1021 tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt atcaatatct
     1081 tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat tacataaaaa
     1141 caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta taccggcggc
     1201 cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa
     1261 ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca
     1321 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat
     1381 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
     1441 attcgccatc ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct
     1501 tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag
     1561 tccagcgcgt ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta
     1621 accggatttg aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc
     1681 gccattatac tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg
     1741 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     1801 agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg
     1861 cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac
     1921 ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga
     1981 ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta
     2041 ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg
     2101 ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac
     2161 ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta
     2221 caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt
     2281 aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg
     2341 ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc
     2401 caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct
     2461 acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct
     2521 caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat
     2581 taacagtcat gcgctactac caactgagct atgccggaat aacatcagcg tggcgacgtc
     2641 ctactctcac aaggggcaac ccctcactac aatcggcgct aagaagctta acttctgtgt
     2701 tcggcatggt tacaggtgta tccttctcgc tatcgccacc acactgtggt gttatctttt
     2761 attgagtaaa ttttgttcac tcaaaactgg atttgaagtc acataagttt ttttccgagt
//
LOCUS       DAJSBC010000161         2794 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSBC010000161 DAJSBC010000000
VERSION     DAJSBC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.166"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            20..400
                     /locus_tag="ONT65_002846"
     CDS             20..400
                     /locus_tag="ONT65_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2330396.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            382..1296
                     /locus_tag="ONT65_002847"
     CDS             382..1296
                     /locus_tag="ONT65_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2330397.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1541..2230
                     /locus_tag="ONT65_002848"
     CDS             1541..2230
                     /locus_tag="ONT65_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330398.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2351..2437)
                     /locus_tag="ONT65_002849"
     CDS             complement(2351..2437)
                     /locus_tag="ONT65_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR2330399.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
BASE COUNT         1037 a          535 c          546 g          676 t
ORIGIN      
        1 tactgaaagg gtgggactta tgagcgaaca acatcaaaat ttggctagcg atcattctaa
       61 aaaatatacc tatcgatccg agccaaaaca aatcagtttt cgagtgagcg aatccgaatt
      121 tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc
      181 caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg ccagacattg cccaggcaat
      241 cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa attgccaagt ggtgcaatac
      301 acaccaacac gacgttgctc ctgatgatgc tcaacgttta tccgaaaacc taaaaatcat
      361 gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa gtcgaaccac ttcatgtagc
      421 cgagcaatca attacgcaga accacgagca accgtcaaaa caggaataaa ctgtgacatt
      481 gattatgcca aaagtgaaat gaaacaaata cgcacgcttt atggaaaaga cgatcgtgta
      541 caagcacact tattgattca gtcctttcga ccaggagaaa ttaccgctga aaaagccaat
      601 caactagggg cagagtatgc gaaaaaaata gcccctaacc accaaatcgc gatttacaca
      661 catacagaca aagaccatat tcataatcac attgtcatta actccgttaa tcttgaaact
      721 gggaaaaaat ttcaagccca tgggcaagca tttttaaaca aatgttatga cctaaatgac
      781 gagatttgtc ttgaacatga cttaacaatc acggaacgag gaaagaaacc cgaaaaacga
      841 accatgtctg aaattaaatt aaaagaaaaa aatgaaccgg tttggaaaga cgaaattcgg
      901 tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa cgtataccca attttgtgat
      961 tcacttaaaa cattcggcat tcattgcttg aatcgcggaa aaaattttac ttacgaattg
     1021 gtccacaaaa agaaaaaagt acgatccaac aaactaggaa aggattacga aaaggagacg
     1081 attttacatg agctggacag acgagaacga acctacaatc aacaacgaac caacgagcta
     1141 cgaagagctc gagctagcat tcgcgaaact gcagaagaag tacgaagaga acaacgaact
     1201 tttacgcctg aacaatcgga aattagaccg actcgaagaa accatgaaca acacgttgaa
     1261 cgaggaacag accaccaaca agaattatcg ctctaaaacg ttaaaagaca tcgaaaacga
     1321 aataaagaga caacgtgcct ttcttgcgcc ttatacccaa gaaaggatac aaactacgat
     1381 ggattcacaa gtcaaaaaac tccaccaagc gctattagac cagcgaaacc aagaacaaaa
     1441 aaaggcttta attcaacgat cgatggatat tcttgccccc ttatgttgct tagtactaac
     1501 attattagcc ttttggttgg tatggggaga ataaacagac atggcattct atttacccta
     1561 tctactcatt tttgccagta ttttaggaat tatttggctt agctataagg cttatcaaac
     1621 aagatattca ttaaaaggct ctaaaattcg atttaagagg tttttattgc caggggttat
     1681 atttacatta tcaatcgttt taagtggttt attcggcgtt ttagagggaa ataaacgtat
     1741 ttcaagaagt attttattag gggatatcac tcaaaagtat gaagcagcat gtgataaaaa
     1801 gaaaaaggag caagtgttaa cacaaaaaat ggaagagttt accacttgtt atgaggaaat
     1861 gaacgatatt tttgtgaatc aagaaaaacg tttgacggat aaaaacatgg aaaaactcac
     1921 tcgtctttat caaaacttgc cagaaaaaca gcaaaaagag gttcaagaca actatgagcg
     1981 aaccaaaaaa gacgtgcaat attttaaaga tacgcagaca gaagaagctt gttataattt
     2041 attcagtgat acaatccctt tttcaactag tgaacaggaa cgaaaagaac ggcaacaatc
     2101 agttacctac gaacgttata aagcactgct tcagcaagca acgaacatac aaaatccaac
     2161 gaaaaaagaa acggcattaa attatctaaa aagtgtcaag gaatggttag atcagcaaca
     2221 gcaaaattga atttgatcgg caatcatgtt aaactaaaca tgcaaatgat caaacattag
     2281 atacacgaaa gccccagaga ggtcggattc tctggggctt tttttcaggt tgatgtcgcc
     2341 tttattgatt ttacttcttg tttcggttat tcaaccaata actgaataaa gagacacaaa
     2401 cttctataag aagggggcaa atgaacaatt caaacattag atacacctcc ttccagtcgc
     2461 aagactgtcg gataggcgac aagttattat atcttaaaaa attaaacttg tgtggtgttt
     2521 ttaagtatcg cttattttag ggttgttagg gcttgccctg accgcctgta aggcgcttta
     2581 aagctagata aggattttta tccaagactg tcttaaaacg gcttagataa gcattcagaa
     2641 ccgctcaaag gcctctttac caacatgttt ttagcaaagg agcctttagg cgactgcgca
     2701 gtacaatgtg agagcgtagc gatcacacct ttttggcgac cgtaggaagc aaggttttgg
     2761 attggcagtt ttgaaaaatg cgcgaacctg ttta
//
LOCUS       DAJSBC010000162         2752 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSBC010000162 DAJSBC010000000
VERSION     DAJSBC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.167"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..443)
                     /locus_tag="ONT65_002850"
     CDS             complement(<1..443)
                     /locus_tag="ONT65_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2330400.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="ONT65_002851"
     CDS             513..863
                     /locus_tag="ONT65_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2330401.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="ONT65_002852"
     CDS             1277..2095
                     /locus_tag="ONT65_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2330402.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="ONT65_002853"
     CDS             complement(2168..2527)
                     /locus_tag="ONT65_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2330403.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          878 a          421 c          517 g          936 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc ta
//
LOCUS       DAJSBC010000163         2730 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSBC010000163 DAJSBC010000000
VERSION     DAJSBC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.168"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1211)
                     /locus_tag="ONT65_002854"
     CDS             complement(<1..1211)
                     /locus_tag="ONT65_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2330404.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..>2730)
                     /locus_tag="ONT65_002855"
     CDS             complement(1201..>2730)
                     /locus_tag="ONT65_002855"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2330405.1"
                     /translation="SEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQTML
                     YRELLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLAS
                     TYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLI
                     SQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYL
                     THPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAV
                     VMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPH
                     GVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFID
                     ASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVD
                     TFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHE
                     Q"
BASE COUNT          769 a          570 c          451 g          940 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtga
     2521 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
     2581 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
     2641 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     2701 cagtcccaat aaataatttt tatattctga
//
LOCUS       DAJSBC010000164         2719 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSBC010000164 DAJSBC010000000
VERSION     DAJSBC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.169"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(173..463)
                     /locus_tag="ONT65_002856"
     CDS             complement(173..463)
                     /locus_tag="ONT65_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2330406.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(599..778)
                     /locus_tag="ONT65_002857"
     CDS             complement(599..778)
                     /locus_tag="ONT65_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330407.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(775..1125)
                     /locus_tag="ONT65_002858"
     CDS             complement(775..1125)
                     /locus_tag="ONT65_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330408.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1118..2443)
                     /locus_tag="ONT65_002859"
     CDS             complement(1118..2443)
                     /locus_tag="ONT65_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2330409.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          823 a          526 c          406 g          964 t
ORIGIN      
        1 attaatcata agaaatatgt tttgcgagca gcaactagaa taatttaaac attattaaac
       61 tgtctatatt gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata
      121 ttacgatatc atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat
      181 gtttttttat ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca
      241 ggagcttttc ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga
      301 atgaagcagg taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt
      361 gtgggtaggc atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt
      421 aaacattgat cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt
      481 tcttttatta ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc
      541 tatttgtaaa aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt
      601 atatatataa cgggaaaata taaaacctaa aattatgata acctgctgca gcttctacat
      661 agaaagagaa gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct
      721 cttctgatag ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc
      781 taatcgactc catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat
      841 tttgtctttt ccaacgtaga tccccagctc atcactacct aagatcttgc caacaatatc
      901 ttctggatag tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt
      961 tatgactgca gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa
     1021 attttgactg gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata
     1081 aaaccctgcc cactttaaac gtccacgatc attgtaatca atcattttta attcccccat
     1141 ttccgcccgc atgaccacca acaagtgtgc ttctagtaat gcttctagcg ctttctaaca
     1201 ttgaagaagc atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt
     1261 tatctaaatt ttcttcgtta atttgctttt gtggatcact aaataaatcc agctgaagcg
     1321 aatcggtgta cacaagcttg ctataggtta ttccaacgtg ccttacttct tgtccttcat
     1381 aatacttatc aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag
     1441 gtattttcat ctggtgtgaa aatcctggtc tcgtttctaa gatcgacgta ccaatgttta
     1501 aatgaacaca tcctgtttta caattatgct gcctgatacg aatggctact tgttccgcca
     1561 tttcttttac caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg
     1621 aattaccata agatttttcc tttgtcggtt ctggcgggat tgctatatct gttcgatcga
     1681 ttccgttagc atggaagtac agctgtaatc caatcacacc taatctattc ttaatcgtat
     1741 aaggattcca gttagcaagg tctctaatgc tcaaaattcc catttggttg agcctttttt
     1801 tcattcgaga accaattccc caaaagtccg tcatttcggg aatattccat actttctcag
     1861 gaacgttttc gtacgtccat tccgcaataa atccttcatt atgctttgct tcattgtcta
     1921 aggctaattt agccaaaagg ggattatcgc cgacgcctac cgtagcaatc aaacctaact
     1981 cttctttaat acgttcttgg atcatttgag cgagcttctt ccttcgctga cttcgtgttt
     2041 cttcagtcgt aaaaagattc agtgattttg tcacttttaa aatcgattca tcgatcgagt
     2101 aaatcagtag atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc
     2161 gcttgatata taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg
     2221 gtaagtcacg tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag
     2281 aagctaatat caatcctgaa ccagtattat cagatcgact cataacaaca agttctgttg
     2341 ttaatggatc taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga
     2401 ttaaaaagta atcatttact ggctcttttg aataatccat cataaataac actccattct
     2461 tccacattat acaaacatat gttcgatgtt gtaaagaaga aatagctaaa tacataaaaa
     2521 aagaagcata ggtatgctat caccctgctt cttcttatga tttgtatgat caatttattc
     2581 tatcacttta ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag
     2641 actgcaaatt agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata
     2701 aaatccttac aacatttga
//
LOCUS       DAJSBC010000165         2703 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSBC010000165 DAJSBC010000000
VERSION     DAJSBC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.170"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(75..1169)
                     /locus_tag="ONT65_002860"
     CDS             complement(75..1169)
                     /locus_tag="ONT65_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2330410.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="ONT65_002861"
     CDS             1358..2119
                     /locus_tag="ONT65_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2330411.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ONT65_002862"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="ONT65_002862"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2330412.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          855 a          542 c          463 g          843 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gcc
//
LOCUS       DAJSBC010000166         2653 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSBC010000166 DAJSBC010000000
VERSION     DAJSBC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.171"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            256..531
                     /locus_tag="ONT65_002863"
     CDS             256..531
                     /locus_tag="ONT65_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330413.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ONT65_002864"
     CDS             544..2616
                     /locus_tag="ONT65_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR2330414.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
BASE COUNT          814 a          414 c          569 g          856 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aac
//
LOCUS       DAJSBC010000167         2646 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSBC010000167 DAJSBC010000000
VERSION     DAJSBC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.172"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..303
                     /locus_tag="ONT65_002865"
     CDS             <1..303
                     /locus_tag="ONT65_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330415.1"
                     /translation="FLCMSYEPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLK
                     DTGVLIGTNSKVYQEVKEKIENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            955..1380
                     /locus_tag="ONT65_002866"
     CDS             955..1380
                     /locus_tag="ONT65_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330416.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1410..1574
                     /locus_tag="ONT65_002867"
     CDS             1410..1574
                     /locus_tag="ONT65_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330417.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2532..>2646
                     /locus_tag="ONT65_002868"
     CDS             2532..>2646
                     /locus_tag="ONT65_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2330418.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDV"
BASE COUNT          877 a          452 c          460 g          857 t
ORIGIN      
        1 tttttatgta tgagttatga acctgctacc aatacaacca caaccagtaa ccaagcaaaa
       61 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
      121 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata tcaagaagtg
      181 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
      241 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
      301 taaaaaaaga aaccgaaaaa taaaaaaatt cctcgatttc tttttaggga tcggtcttcc
      361 agcaagattt gcaagttggt accaacttgc gaaaaaaaaa agaaaaatcc gcttctgcga
      421 attcttcttt tttcattttg gggcgctgcc ctaatcccta agtcccggtt attcaatagc
      481 atggaaactc aaagaataag acgttccctg gaataaaaaa aaggctgctc gttcgagcag
      541 tccatacata ggaaaatgaa aaaacgacgt cccttactga gaaacgctat taaactttta
      601 acaaatcctc taaaaaataa caagtcttta aaaagtgacc ggtaaaaatg cacaaagaac
      661 taaaaaagag aaattgcatc aaatcaagca ttagaagcta gtaactagct agaccaattg
      721 aaaatcacaa agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat
      781 tatgtcaaca agaatttaat ataacatatt tttctagttt gttataacaa tgccactcta
      841 acaaaaatgt aagcattatc cttattaaaa gctccttgac aaggactata ccttttattt
      901 tatgattttt acatagaaaa cgttttctct attttttgtt tagaggtgat ttttatgatt
      961 tgcaagaaca agaaaaaaat tattgctatt attgctagtt tctctttgat ttttagtact
     1021 gcatgtactg tcttattaac aaataatgct aatgttgaag ctgcaagctc ttcttacaaa
     1081 cataatcatg ctggttatag atgttacaaa atgagttatt atgtaagtcc ttctaaatgc
     1141 aaacaattgg ctcgccaaac taataaactt aataccgtct cagctattgc aaactttatt
     1201 ggactatctg gattaacacc ggggattata tcattagttt tcggatcagc agttagtgct
     1261 aataatgtat ttgttacagc cgctagaaga ggaaaaggcg ttcaattaaa ttatattgcc
     1321 cattttagta ataccactac ctataattat agttctaacc aaaattatgt tataaaataa
     1381 ttaaaacttt aaactaggag gaatgcttta tgaaattaac aaaaattgct gtaattttag
     1441 ctgttattgc tggcattatt ttatattttg tattgcctgc taatcaattc ttcctttact
     1501 ttggaatacc tgcgatttta atagtaaatg gcgtaagaat ctttgaacaa cgaaaaaaat
     1561 gtagctctac ctaatcgtta caaaacaaca atttcacctt cggtgaaata agcgaaactt
     1621 tacaaaaagt ggctagcaaa tgctagcctt ttttgtattg tagagcgtga attttgaggg
     1681 ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa atacctccgt
     1741 atttggctag caatccttct aaaaaataca cctatcgatc agagtcaaaa caaatcaatt
     1801 ttcgagtgag cgaatccgaa tttgcaaagc tgaagctttg caaatgagtg tgccggcttc
     1861 cgtaaaaata catatggcta taatttgcgt tttaaggcgt tttaaacgtt ctggtatata
     1921 attataccaa aacaacttta aaacgcctta gatctaattt tagaggtctt atagaacatt
     1981 ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttgagaaca agataagggt
     2041 tttatactaa gactgtctta aaacggctta aacaaacgct cagaactttt caaaggagtt
     2101 ctttatgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagtat aatgtgagag
     2161 cgtagcgatc acacattttt agtgaccgta gggagcgagt ttcccagttt agaaaaattc
     2221 gcgaacttgt ttgaggaaaa aagcaggagt tttttaggcg ggaaattttg cctcctcttt
     2281 tataatctct ttttaaacct ctttttaaac ctctttaccg tcggctgtat gcttactctc
     2341 ccaaggtgtg cagaatcttt tgggggtctt tttgtctgtt tttaggggtc tttttgtctg
     2401 tttttagggg tctttttgtc tgtttttggg ggtctttttg tctgttttta ggggtcttta
     2461 attaggggct tgacccctat tagaaagctc tttataattt tgttaagtag ttaatcttga
     2521 aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat acgattcctt
     2581 tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tatagtatct agaatgcgtg
     2641 acgtag
//
LOCUS       DAJSBC010000168         2528 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSBC010000168 DAJSBC010000000
VERSION     DAJSBC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.173"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            11..2383
                     /locus_tag="ONT65_002869"
     CDS             11..2383
                     /locus_tag="ONT65_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330419.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1013 a          399 c          557 g          559 t
ORIGIN      
        1 ggaaacggac atggcagaag aaaaagaacc actagacaat agtcgcttgg gaaaatccaa
       61 aagaaaatta gtacgattgc aaaatgaatt aaacgaacaa attgagaaga tgtttgaaca
      121 ccagagaaag accaacggtc agccaatgaa cgataagaga aatgggcatt cttggtttag
      181 gcaacaagaa cgtttagaaa acaaagtgca tagtctgaga gaagagatca agcaacaaga
      241 aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta
      301 cggcggcctg gatatgacaa tcgaaaatat tccaagaatc aaagaagaaa tcgaacgctt
      361 tgaaaaaggt gaatcgactt tttcagccgc tacgatccga aaataccaac ggaaattgga
      421 aacattagaa cagctgaaag aaagatcaga aaaaggaaaa gaaaatcttc ttcctgaagt
      481 acaagcaatt attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt
      541 tttgaaagac tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc
      601 aaaaaaatac caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga
      661 ggctgcgcca actgattctt tgaaggagaa agaaaacgat catttctatc gagtagaatt
      721 caacgaaaat gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga
      781 attgatcgat caactcaaaa cgttggataa ccaatacgcc gcagaagcag gttattataa
      841 attctatttt gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg
      901 ggatggcgtc aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt
      961 acctgaaaag gaacaaaaac aaacggtcaa aaatgaacct gtttcgaaag aacaaatgca
     1021 gtcgaaagaa acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa
     1081 gcaagaagtc aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc
     1141 tcctgaagac gtgaaagaac ttttgaattt tatgtctcgt tttccagaac gatcctttag
     1201 aaatcaatta ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgctgc
     1261 gttgaaaaaa gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag
     1321 aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac
     1381 accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta
     1441 cacgattgaa aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa
     1501 aatctttccg aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca
     1561 agctgggatt caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag
     1621 tgaagtctat caaagagaat taggacaagc acggggggca tatgttcgta acgaatttac
     1681 tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag
     1741 tattcatgaa ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa
     1801 agaattgcag gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacttc
     1861 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
     1921 ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
     1981 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
     2041 agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc
     2101 tgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
     2161 aacgttacat ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt
     2221 aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct
     2281 aggaaattca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga
     2341 aatctattat gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact
     2401 atctgaaaaa cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat
     2461 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
     2521 ggctgtag
//
LOCUS       DAJSBC010000169         2436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSBC010000169 DAJSBC010000000
VERSION     DAJSBC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.174"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..272)
                     /locus_tag="ONT65_002870"
     CDS             complement(<1..272)
                     /locus_tag="ONT65_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330420.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ONT65_002871"
     CDS             complement(309..446)
                     /locus_tag="ONT65_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2330421.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ONT65_002872"
     CDS             complement(448..894)
                     /locus_tag="ONT65_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330422.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ONT65_002873"
     CDS             complement(891..1040)
                     /locus_tag="ONT65_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330423.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="ONT65_002874"
     CDS             complement(1054..>2436)
                     /locus_tag="ONT65_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330424.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DAJSBC010000170         2407 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSBC010000170 DAJSBC010000000
VERSION     DAJSBC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.175"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..1150
                     /locus_tag="ONT65_002875"
     CDS             5..1150
                     /locus_tag="ONT65_002875"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR2330425.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ONT65_002876"
     CDS             1321..1779
                     /locus_tag="ONT65_002876"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR2330426.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ONT65_002877"
     CDS             1938..>2407
                     /locus_tag="ONT65_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR2330427.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAJSBC010000171         2400 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSBC010000171 DAJSBC010000000
VERSION     DAJSBC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.176"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            207..2069
                     /locus_tag="ONT65_002878"
     CDS             207..2069
                     /locus_tag="ONT65_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2330428.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ONT65_002879"
     CDS             2069..>2400
                     /locus_tag="ONT65_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2330429.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAJSBC010000172         2388 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSBC010000172 DAJSBC010000000
VERSION     DAJSBC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.177"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..596
                     /locus_tag="ONT65_002880"
     CDS             <1..596
                     /locus_tag="ONT65_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2330430.1"
                     /translation="YKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRK
                     GTDYRNNYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLAT
                     YILRYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESAN
                     DMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            597..1349
                     /locus_tag="ONT65_002881"
     CDS             597..1349
                     /locus_tag="ONT65_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2330431.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1354..2301
                     /locus_tag="ONT65_002882"
     CDS             1354..2301
                     /locus_tag="ONT65_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR2330432.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          846 a          307 c          460 g          775 t
ORIGIN      
        1 attacaaaaa tatttatgtt gctgctccac tgaggtataa aaatatcatg gaagttgttc
       61 gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc tatttgttct acttatcttc
      121 ctgattggag aaaaggtact gattatagaa ataattattc tgcctttaaa tctatggggg
      181 gaggagtaga attagactgt atacatgaat tggattatat tataaaccta tttggtttcc
      241 ctaaatcagt aaaaaaacag tttggtaaat attcgaatct tgaaattaac agtaatgatt
      301 tagcaacata tatattgaga tacgatgata aaattataga aattcattta gattattttg
      361 gaagagttcc tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact
      421 tattaaataa taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta
      481 atgatatgta tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa
      541 actggaatga tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg
      601 aaattttatt tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga
      661 aatatttggc aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac
      721 aaaaacatcc tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag
      781 aattgacgac taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta
      841 cagatactgt agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag
      901 ataatatatt tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta
      961 atcttgaaga attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag
     1021 ttgtttctgc aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta
     1081 ctatagttaa taaatcaaga tacacatcta ggcaacagac acctgaagtt tttgaaatga
     1141 atgcctcaat gtattctttt aaacctgaat ttctaaaaaa aagtaattat atttttgatg
     1201 ggcaatgtgg gataataaaa atgagagatt atctaatttt agatattgat agtgaagaag
     1261 attttaaatg gttagaattt ttgtttccta aatttttaga agattcttcg gagcttcaag
     1321 atatctataa ttatttatat cggaggtaaa aggatgagct gttatcttat caccggtggg
     1381 gctggcttca ttggttctac cttagccaat catttaggag aagaacatgt ggtaatcgtc
     1441 gtcgatgact tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa tatcactttt
     1501 attgagggag atgtcgctga gaaatccttg atggaagaga tcatgaaagc taataaattt
     1561 gattatatct ttcatttagc agcagttgct agtgtggcag attcagttgc ccgaccagta
     1621 gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt tagaattgat ccgcaagtat
     1681 caacctgatt taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg
     1741 actttaccaa aaaaagaaga gtccgtgatt cgcccattga cgccttatgc ggttgataaa
     1801 ttcgctgcag aacaatatgt gctagattat tgtcatttat atgatgtacc cggtagcgca
     1861 gttcgttttt tcaatgtcta tggtccaaat caaaatccga attctccata cagtggtgtg
     1921 atttctattc tcgtagatcg ctataagaaa caaatcgtag gagaagcaac ttcatttaca
     1981 ttatttggtg atggaagcca gtctagagac tttgtttata tagatgatgt aatccaagca
     2041 ttacttttag ttgcgaatga ggaagctgca ctggggaaac aattcaatgt tggaacgggt
     2101 aaagcaacca cacttttgga attgattcat gcaatcaatg aaattctcgg tgtagaattg
     2161 acacttgaat acaaagaaga gcgttcgggt gatatccatg actctctagc agatattact
     2221 aagatccagt caattggcta tcaaccgaaa tatggtattt taagtggaat gactaattat
     2281 ttgaaaactg aaatcaatta aaacatacta agataagatt agagggtaaa ttcaggctct
     2341 ttgtcaataa ggactgatga gttttgtaaa atcaaatctg ggatagaa
//
LOCUS       DAJSBC010000173         2276 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSBC010000173 DAJSBC010000000
VERSION     DAJSBC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.182"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(62..265)
                     /locus_tag="ONT65_002883"
     CDS             complement(62..265)
                     /locus_tag="ONT65_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330433.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(275..697)
                     /locus_tag="ONT65_002884"
     CDS             complement(275..697)
                     /locus_tag="ONT65_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330434.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            935..1549
                     /locus_tag="ONT65_002885"
     CDS             935..1549
                     /locus_tag="ONT65_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330435.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1714..>2276)
                     /locus_tag="ONT65_002886"
     CDS             complement(1714..>2276)
                     /locus_tag="ONT65_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330436.1"
                     /translation="NTRDRFISIITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKLL
                     GSMFKGYFNIEFPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFF
                     YKINDSFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKE
                     LYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          747 a          380 c          338 g          811 t
ORIGIN      
        1 tttatgaaca ctttcaatta gaaaaaaaga atgtcgacta gacactcttt ttttaaaatc
       61 attatttttc tttttgatat gttttttggt ctcttttttt tcttttttct tcagaatctt
      121 ctagattaga aattgatttt tgaatttttt gtgtctcctg taaagttcgc tgaacttgta
      181 aagctgattc ttctttcggt aaattaattt tacttagaat atcgctatct tgataaaaat
      241 ttggtgtgtt cacaaattct ggcataaaaa atccctactt tctagttaag gttcctgaat
      301 aagtcgcaat ccaattgttt ccagctgatt tataactttg taaatacaat tttccatagt
      361 accaataacc atttcttttt tctttccaat tccgtgattg aggaggtcta gaaggccaac
      421 caactttatc aaaaatcatc gttcctgtca ctcgtatcgt tttagaagct gctagagcaa
      481 gtaatggaga aactattaaa taattttcag taacagttaa gtcgttagtt gggttaccaa
      541 tggctattcc gtccctatct aaaggagtta attcttggtt attatctact acagtagttg
      601 gttcatttgt actatcagca aatgtaggaa ttgtagttcc agttacacta attccaatag
      661 ttaaaatacc taaagataaa ataaacttat ttttcattgt ctatcacctc gaaatcaaaa
      721 ataagaaaac gttttcttaa taaatcttaa cagataaatc aagttatata ctagaaaatt
      781 cataaaatat atttttaatg tcataatcgt ttaaattttc ttgattgaca tttatttaca
      841 aaaaatattg ccatttaaaa agaaaagtga catatggctt cctcgtgtat gattagggtg
      901 tattttaaat agttgcagaa agtaggtaaa agttttgaga gttgcataca tacgtgtctc
      961 atcgatcgat caaaatgaac aacgccagat tgaagaaatg aaaaaattcg gtgcagagag
     1021 aattttcatc gagaaacaat ctggcgcaac gattactcat cgacctgttt tccaagaagc
     1081 attagattac gtccgagatc aagatatttt tattgtggaa gctattgatc gacttggaag
     1141 aaattatgac gagatcatcg cttcagtcaa ttacttgaag aagaaaaatg tgaagctgat
     1201 cattactagt ttaccgatca tggctgaagc aattggcaat cctctcctgg ataaattcat
     1261 taaggatctg atcatccaaa ttttggccat gatcgctgaa caagaacgga ctgaatcgaa
     1321 acgtcgccag gcacaaggaa ttaagatcgc aaaagcaaac ggcgtctaca aagggcggcc
     1381 caaattatac agtgcagacg caaaagatcc ccaacgtcgc cttgtttaca aaagcatcgt
     1441 tgaagatctt aaaaatggtg ttgcgattgc taagattgct aaagactaca atgttacgag
     1501 acaaaccgtg tatagaataa aaaaagacag catggtcaat gacaaatagt tgatcatgct
     1561 gcttttatta tcttctcatc tttaatttcc tacatataat tactatctat aaaattaagt
     1621 cttactcaga acagataaag cataaaattc caacaaacca ttttttactt aactaaataa
     1681 agagtctgtt aatataatct tcacttattt acgttaatcc cctactgtat ttgttggttc
     1741 cttatctata ttttctacaa aatccttatt atcttgtgat ttagcttcgt ataattcttt
     1801 gtatgtcgac acattggtta tactaattcc tatagctagt tcttgagact ctacttttct
     1861 ctctagctcc tttgacaaag tgatttgaga tttatttggt tccgttggag tcaattctat
     1921 agattcttca taaaaatctc tatacataaa gctatcatta attttataaa aaaaattaaa
     1981 tgtttttagc actgccatat aacattcaaa acattcttct atagttggtt tacttccttg
     2041 aggagaatga accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc
     2101 tttcggaaac tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg
     2161 gggagattgt aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc
     2221 ctttacccct atttcacagg ctgtaattat cgaaataaac ctatctctag tatttg
//
LOCUS       DAJSBC010000174         2233 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSBC010000174 DAJSBC010000000
VERSION     DAJSBC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.183"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            221..838
                     /locus_tag="ONT65_002887"
     CDS             221..838
                     /locus_tag="ONT65_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330437.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            879..1178
                     /locus_tag="ONT65_002888"
     CDS             879..1178
                     /locus_tag="ONT65_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR2330438.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1212..1379
                     /locus_tag="ONT65_002889"
     CDS             1212..1379
                     /locus_tag="ONT65_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2330439.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1424..2029)
                     /locus_tag="ONT65_002890"
     CDS             complement(1424..2029)
                     /locus_tag="ONT65_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330440.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          738 a          362 c          418 g          715 t
ORIGIN      
        1 atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat ataatcatgc
       61 gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga ttttctttgt
      121 ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt aatctgattg
      181 ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat ttttttatat
      241 ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga aggaattagg
      301 aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta aacgccctga
      361 atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac aatcattaga
      421 tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa
      481 gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt acaagcaact
      541 agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct atatggccga
      601 agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg cgaaacaaaa
      661 gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg cgatctattc
      721 tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg gcatgaaacg
      781 aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact tttcttgata
      841 gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg
      901 tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt ttggttcact
      961 ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc
     1021 agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa accgcgcggg
     1081 acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt tatgcgttaa
     1141 ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa tttacaagga
     1201 ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata actagtttta
     1261 atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa agtgtttatc
     1321 aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat ggaacataga
     1381 aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa tcattcgctt
     1441 tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg
     1501 gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt tgcggatctg
     1561 ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt tgagccaata
     1621 atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca atataactta
     1681 gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca gtattaaaat
     1741 ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata aaagcaactg
     1801 tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg atgacgcaat
     1861 caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta tctttaccac
     1921 tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc aattgtcgat
     1981 ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata aaaaactcct
     2041 ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc tatcatagca
     2101 tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt taaatggatg
     2161 aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt acagaacaat
     2221 taaaccaatt ttt
//
LOCUS       DAJSBC010000175         2232 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSBC010000175 DAJSBC010000000
VERSION     DAJSBC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.184"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..544
                     /locus_tag="ONT65_002891"
     CDS             <1..544
                     /locus_tag="ONT65_002891"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2330441.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="ONT65_002892"
     CDS             complement(900..1472)
                     /locus_tag="ONT65_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330442.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="ONT65_002893"
     CDS             complement(1488..2093)
                     /locus_tag="ONT65_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR2330443.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DAJSBC010000176         2156 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSBC010000176 DAJSBC010000000
VERSION     DAJSBC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.185"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            17..1045
                     /locus_tag="ONT65_002894"
     CDS             17..1045
                     /locus_tag="ONT65_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR2330444.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1101..1319
                     /locus_tag="ONT65_002895"
     CDS             1101..1319
                     /locus_tag="ONT65_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330445.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1390..>2156
                     /locus_tag="ONT65_002896"
     CDS             1390..>2156
                     /locus_tag="ONT65_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330446.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLS"
BASE COUNT          824 a          249 c          385 g          698 t
ORIGIN      
        1 aaggagtatg ataataatga atagcaaaaa acttcagatg ggtgaaattg ttagaggtcc
       61 tagtcaaata tctattgatg ttacatataa atgtaatttt agatgtaaac attgttacaa
      121 tgcgagtggt ggagacaatt gttttatgga taatgagttg actgatgatg aactgttaga
      181 acttacaaaa gatatatgta atttaaagcc atataatatt tgtttctgtg gaggagaaac
      241 tttactgaga aaaaaaatag ttttatcttg tgccacttta ttaaaagagg caggtattcc
      301 taatattgct attgttacta atggatcatt agtaactcag gatgttgcaa aagaattatt
      361 caaagcaggt attaatcgat ttcaagtaag tttagatgga aaaaatgcta gtagccatga
      421 agcacttaga ttatatccaa acagttttga aaaggcaatt aatgctatca aattatttag
      481 agaattgtcc gaagaagtgg atgttgcttt ttgtcctact agttttaatt acaatcaatt
      541 acgagaagta tatcaactag taaatactct taaagtgaag tctttaagaa ttcaaccatt
      601 tatgcttagt ggtaggggaa tgcaaaatag ggaaatagtg ccaactgttt ctcaatatag
      661 aaaactagtt tctgatatta actatattaa aagaaatgaa gattatacca agatagattg
      721 gggagatcca attgatcata tcatccgaca cagaacttta ttgaaagatt tttcgaattt
      781 tgtttctatt aggtcagacg gaggattaat ggcatcacct tacttgccaa ttcaagttgg
      841 aaatattaaa tgtcacagtc tttctaaata ttggcgagca ggactttata aagttttttc
      901 tttagattta gttcaagata tagctactca agtttcttca atacaagata tgagtaaaga
      961 attaaaaaaa ggacatatta aatggcttaa tgaagatata gaaattgata ttctcgatga
     1021 atacaaacat gatattttaa tttaggttta tcattggaaa gtttagataa acaaagtact
     1081 tttagaaagg agggaaaaaa atgatttctg aacctcaaat gaataatatg tatgaaagcc
     1141 caactataat gggagctggt gaagctgcgc caagagcttg ttcagcaatt atggtgtgtg
     1201 tgttattagc atctgcagga gtaggtatcc aagcaggagc ggtgtatact tatgctgtag
     1261 ttgctacagt agctgtagct gcagccgcag tttggaaaac agtaggatta gttggttagg
     1321 aattaatagg agactaactg tctcctattg attccaatga ttaagctaag gaggaatttt
     1381 aagaaaataa tgaaatacaa taatttaaaa ccgaaattac ttaatgagaa catagaatat
     1441 ataagagaag aaaattatgg atatttaacc atagttccca aaaatggaac tgaattgaaa
     1501 gataacgttt tcaatccaac agtaagatat atattggcta actgtactgg aaaaaacagt
     1561 gtaaaaaaaa tagcgaatgg tttaaaaaag aagtttccta gtttagaata tgaaaaattc
     1621 tatcaagatg ttgtaggaac tattaaattt ttcaaacact ataatattat tgatatagga
     1681 gattatagta tggaagaaaa gaaaatatat aaaattgaac aaaatttaga attagaatta
     1741 ctaaatgaat cacattatga agaaatttat aattttatca cagatcatcc tgaaagtatg
     1801 tattttcatg aagatattta tcatcaagga aatagtaaag aattagaagt tgtccttaga
     1861 tataatttat tttcgtttac gagtgaattt tactttttaa gacataaaga gagaattgta
     1921 ggaatggttt ctataattgt taatccattt caaaacttat cctacagtat agtaaattac
     1981 ctcatatgtg agcctgcata tactacgact attttgacaa cagtattaga aaatattaac
     2041 gaagtttcaa ttttagaagt aaagaagcta gtgattcatt tgctaaattc aacagaagat
     2101 gaatcactaa tcccctattt aaaagattca cattttttta aagaagcggt actatc
//
LOCUS       DAJSBC010000177         2122 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSBC010000177 DAJSBC010000000
VERSION     DAJSBC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.186"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..914)
                     /locus_tag="ONT65_002897"
     CDS             complement(<1..914)
                     /locus_tag="ONT65_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR2330447.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ONT65_002898"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ONT65_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR2330448.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          717 a          374 c          412 g          619 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aa
//
LOCUS       DAJSBC010000178         2103 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSBC010000178 DAJSBC010000000
VERSION     DAJSBC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.187"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            169..1539
                     /locus_tag="ONT65_002899"
     CDS             169..1539
                     /locus_tag="ONT65_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCR2330449.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="ONT65_002900"
     CDS             1560..1844
                     /locus_tag="ONT65_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330450.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cat
//
LOCUS       DAJSBC010000179         2079 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSBC010000179 DAJSBC010000000
VERSION     DAJSBC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.188"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(243..464)
                     /locus_tag="ONT65_002901"
     CDS             complement(243..464)
                     /locus_tag="ONT65_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330451.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="ONT65_002902"
     CDS             complement(562..807)
                     /locus_tag="ONT65_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330452.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="ONT65_002903"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="ONT65_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          707 a          361 c          300 g          711 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgt
//
LOCUS       DAJSBC010000180         2065 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSBC010000180 DAJSBC010000000
VERSION     DAJSBC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.189"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1008
                     /locus_tag="ONT65_002904"
     CDS             <1..1008
                     /locus_tag="ONT65_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330453.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ONT65_002905"
     CDS             1025..1174
                     /locus_tag="ONT65_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330454.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ONT65_002906"
     CDS             1171..1617
                     /locus_tag="ONT65_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330455.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ONT65_002907"
     CDS             1619..1756
                     /locus_tag="ONT65_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2330456.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ONT65_002908"
     CDS             1794..>2065
                     /locus_tag="ONT65_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330457.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAJSBC010000181         2042 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSBC010000181 DAJSBC010000000
VERSION     DAJSBC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.190"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            200..742
                     /locus_tag="ONT65_002909"
     CDS             200..742
                     /locus_tag="ONT65_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330458.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ONT65_002910"
     CDS             898..1347
                     /locus_tag="ONT65_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR2330459.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2042)
                     /locus_tag="ONT65_002911"
     CDS             complement(1377..>2042)
                     /locus_tag="ONT65_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR2330460.1"
                     /translation="RRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          733 a          323 c          392 g          594 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
     2041 cg
//
LOCUS       DAJSBC010000182         2002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSBC010000182 DAJSBC010000000
VERSION     DAJSBC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.191"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="ONT65_002912"
     CDS             <1..378
                     /locus_tag="ONT65_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2330461.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="ONT65_002913"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="ONT65_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2330462.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2002
                     /locus_tag="ONT65_002914"
     CDS             1859..>2002
                     /locus_tag="ONT65_002914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330463.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DAJSBC010000183         1974 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSBC010000183 DAJSBC010000000
VERSION     DAJSBC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.192"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            31..786
                     /gene="fabI"
                     /locus_tag="ONT65_002915"
     CDS             31..786
                     /gene="fabI"
                     /locus_tag="ONT65_002915"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR2330464.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(896..1909)
                     /locus_tag="ONT65_002916"
     CDS             complement(896..1909)
                     /locus_tag="ONT65_002916"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR2330465.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT          575 a          417 c          370 g          612 t
ORIGIN      
        1 aatttttatc agaaacaaag gagagtcatt atgtcatttc tagaaggaaa aaaaatcgtc
       61 attatgggcg tcgctaacaa aaaaagcatt gcttggggtt gtgctgaagc aatgttgaaa
      121 caaggggcag aaattatcta tacttaccaa aatgaacgaa tgaaaaaatc attagtcaaa
      181 ttggtcggcg aagaagcatt cactgtagaa tgtgatgttt ctagcgacga aagcatcgaa
      241 aaagcaatga atgccatcgg tgaatatgct ggggagatcc acggccttgt ccatgcagtt
      301 gcttacgcca acaaagaaga attgtccgga aatgtgtctg atatttccag agacggctat
      361 caattagctc aagatatcag cagctactct ttaattgctg tatcaaaata cgcacaaccg
      421 tttcttgcac caaacagcgg gatcgttagc atgacttatc taggttcaga acgcgcaatc
      481 ccgaattata acatgatggg tattgcgaaa gcttctcttg aagctgctat ccgttatttg
      541 gcagctgagt tctcgcctaa aggaattcgg gtcaatggaa tctcagctgg ggcaatcaaa
      601 acattagctg tcacaggtgt taaagactat caaaaactga tccagttatc tgaatctcgt
      661 acgcctgatc agcaaggtgt gacgatcgaa gaagtcggta atgcttgtgc tttcttaatc
      721 agcccacttt ccagcggaat catcggtgat attatctacg ttgacaaagg tgtccatctt
      781 tcataaactt ttgttgcatt ctttaaagta aaacatataa aaatcaggaa aaaacttttt
      841 aaagtctctt cctgattttt tctttttacg tttttttcaa ctcattactt tgaaattatt
      901 ccacgacttc aatccagcct tctggtgctt ctatgtctcc aaattgaata cctgtcaatt
      961 catcatacaa ccgttttgtt actggtccta cttccgtttc actataaaat acatgaagat
     1021 tgttattgtg ataaatccca ccgattggag aaataacagc cgctgtacca catgcaccag
     1081 cttctgcaaa ttgatctaga tcatcaatga aaatatctcc ttcaattgct tgcagtccta
     1141 gtcgttcttc agctaactgc aacaaggaat atttcgtgat acttggtaag atagagggcg
     1201 attgcggagt gatgaatttt ccgtcttttg taattccaaa gaaatttgct gcacctactt
     1261 cttcgatttt cgtatgtgtg gctggatcaa gataaacaca atctgagtaa tccagttctt
     1321 ttgcttctgt tcctggaagt aaactcgctg cgtaatttcc gccgactttt gctgcaccag
     1381 tccccttagg agcagcacga tcgtaatcag atacaataaa attcgttggt tttagtccac
     1441 ctttgaaata cgctcctact ggcgtacaga agacgacaaa taaatattct ggagctggat
     1501 ggacaccaat gttttcaccg accccaatca gtaaagggcg caagtataat gtagcgcctg
     1561 ttccataggg cggcacaaaa tcttcatttg cttttaccac ttgcttgaca gcatcaacga
     1621 acatcttttc agggaccatt ggcatcagca atcgtctagc actttgattc atccgtcgac
     1681 tattttgttc aggtcgaaag agattgacct tgccgtcttt tcgacgataa gcttttagac
     1741 cttcaaaaca ttgctgacca taatgaagcg ctggagagcc ttcatgaatc gtcaggtaat
     1801 tatcttctgt caattctcct tcttcccatt gtccgtcttt ccatttggca ataaaacgcc
     1861 aaggagtttt gatataagaa aaacctagat tgttccaatc gatttgcata tacttaccac
     1921 cttcgttatt ttctccacat tttatcatta tccatattat ttgtcatttt attt
//
LOCUS       DAJSBC010000184         1973 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSBC010000184 DAJSBC010000000
VERSION     DAJSBC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.193"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..867
                     /locus_tag="ONT65_002917"
     CDS             <1..867
                     /locus_tag="ONT65_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2330466.1"
                     /translation="VGDKKVQFTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFG
                     RRSADGLQRERFVLFNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNEL
                     ESSYSKTMFRLLKQYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAI
                     FKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYS
                     EKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKK
                     GLLDNLQNLFKE"
     gene            877..1374
                     /locus_tag="ONT65_002918"
     CDS             877..1374
                     /locus_tag="ONT65_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330467.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1750..>1973
                     /locus_tag="ONT65_002919"
     CDS             1750..>1973
                     /locus_tag="ONT65_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330468.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNIL"
BASE COUNT          814 a          323 c          306 g          530 t
ORIGIN      
        1 gtaggcgata aaaaagtaca atttacattc gaacaattaa aagatttaag caattataaa
       61 gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat
      121 ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt tttatttaat
      181 caatttaaaa tcgatggaaa agctgatatt ccttttgctg aaatacaagt ccatgagaaa
      241 gccttaccct tacttaataa tcttgaagaa tgggttcgat attctctcca acaattcaat
      301 gaattagaaa gcagttattc caaaaccatg tttagattat taaaacaata cagaaccaaa
      361 ggaattgcct atttttcaaa agaagacttt catgaattac ttgatatccc taaaagttat
      421 agagaaagtg atataaataa aaaaattata aagcctattc gccaagaact aaccgcaatc
      481 ttcaaaggtc tgacaatcaa aaagaaatac ggaaaaggaa gaggaaaacc tgtcattggc
      541 tatcaattca cgtttaaacc tgaaataaaa aaagcagacg actttaataa acacgcacca
      601 acaaaagaag aacaaaataa agctttagaa ctagttaatc aacaaattta ctctgaaaaa
      661 gaccccactt cattacaaga aaaaatcaaa gaacagaaac gaaaattaga acttttacaa
      721 caattagaag agctagaaag aaaacaaaat aatctgaata aagaaaataa ggagattaac
      781 gaacaaatac cagataacaa ttccgatatt cccccagaat taaaaaaagg cttactagat
      841 aatcttcaaa acttatttaa agaataaggg gtttttatga cagataaaac tatcaaagaa
      901 ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt atcaactgag gaaattacct
      961 agtaatttta cctacaaaaa aggaaatatt atctacctga aaaaagaagc agtaacaaca
     1021 attacaaagg ttttatcagg taatgagccg ggtaacttac ctagtgaatt acctgattca
     1081 ttacctaccg attacctact caaggagatt gaaactaaga ataatcatat tgaagaaaaa
     1141 gataggcaaa ttgaaaaact tcaactagct ttagaaacac aacaaaaatt actcgaccag
     1201 caacaacaac tcaccctgca agccaaccaa caaatcgaga aactgcagga acaactacag
     1261 ttgacttata cggaagaatc ttcggaaaat aaatcaacga ctctttctga agaaacagaa
     1321 tcgatagaaa aacaatcaaa gacggagaaa aaatggtggc aattctggca ataaaaccag
     1381 caatttaaat taataaaaat tgctggtttt attcttcttt ttgcttttgc gagcagagca
     1441 acatgggccc atgttgcgat attttggtgg cgccccaatc cccgtttttt cgttgaatct
     1501 tactcggact gcactcgttt tacagctgat ttttctgaat tttgaaagag cataaggaaa
     1561 cgtggtatca ctttcttacg aaaggtgcta gataagctgt gcctatcacg tttctcagaa
     1621 aaaaatttgc tatcactttt ttgccattgt ggctaggcta tgtatagtgt tttggcgaaa
     1681 aatagctata caaatactat atttgtatga tatacataaa ttatatactg atacgaaagg
     1741 aaaatttcca tggacatttt agttcgaaat atcaatccca aaattattac aagaatagat
     1801 aaacgttgtc aggaactaac aaccaaaacg ggcaaaaaat ggagtcgaaa tcaataccta
     1861 aaaattttaa tcgaaaacga ttttgaccac gaacttatga attacaaaaa agatcaattc
     1921 gatttattat tcgaaaaatt tattgccatt caagcagaaa atacgaacat act
//
LOCUS       DAJSBC010000185         1799 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSBC010000185 DAJSBC010000000
VERSION     DAJSBC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.194"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ONT65_002920"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ONT65_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2330469.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAJSBC010000186         1721 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSBC010000186 DAJSBC010000000
VERSION     DAJSBC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.196"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..291
                     /locus_tag="ONT65_002921"
     CDS             <1..291
                     /locus_tag="ONT65_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR2330470.1"
                     /translation="KLKDINSIDNYYIQFKGFDFNSSTEFKLALYAQFFIEKLNRFQE
                     IVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
     gene            298..810
                     /locus_tag="ONT65_002922"
     CDS             298..810
                     /locus_tag="ONT65_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330471.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1081..1704
                     /locus_tag="ONT65_002923"
     CDS             1081..1704
                     /locus_tag="ONT65_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330472.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          679 a          205 c          282 g          555 t
ORIGIN      
        1 aaattaaaag atataaattc aattgataat tattatatac aatttaaagg ttttgacttt
       61 aatagctcta ccgaatttaa gttagcactt tatgcacaat tttttattga aaaattaaat
      121 aggtttcaag aaatagttga agatagtgat tttaacactt ttaatagcca taaaccgatg
      181 agaggtactt atataaaatt tctcgaaaat tataatgatt taaaatctac caataattat
      241 caatttggaa acttgtctta tgagatgata agtaaagttc taagtttgtg atgataaatg
      301 aaattaaata gtataaatga tttatttgct attataggtg ttattgctaa catacttttt
      361 tcgtgtttga attttagatt tactcaaaag aacaaaaaga acaaactcag tatagaacaa
      421 atgaaaaaag acattgaaac tgatttaaaa aatgaccata aagaaagaag aaaaattgaa
      481 gaatatactt ctttaatgga aaagtgtaaa gcaactgatg caatttttag ctttttaaga
      541 aaaagtgatg agataatcac acgtgaaaat atagatttag aaaatgagat tagtaaactt
      601 gaatctatct tttccgatat aaattctctt aagtatttaa tcgtcgaaga aaattcggat
      661 agctttttag aatttgaaaa atatctaaca gatcttatag ataatagaaa taatgaatta
      721 atgagacaaa acattgcaag attccttact aaattagttt ctgaatggaa aagtattatt
      781 tctcaaaata tggataaatt aagatattaa ggatgttagt tttaaaataa actaatgtcc
      841 ttatttttgt gttcacattt ctgttaccta agaaatactt aaaggaggtg atgtagccag
      901 caacgactac aacttgaacc cgctatctac agtttatttg ttgtttcttt tgggtatact
      961 ctaatggtac aaatccatat ttgcttaaag cgttgatttt tcagcggttt ttctttattg
     1021 caaagtgtac cctaagacct gtatactaat agtaaataga ttggatagga atgatggcaa
     1081 atgtatttag cttatttaag agtttcttcc gacgatcaaa gtttagctcg gcaacataag
     1141 ctaattgaaa attggtcagt caaaaataat attgctgaag acgatttaaa aattttcgag
     1201 gaaaaagtct ctggaaaaaa tattgaaaac agaccagagc tgcaagagct tatttctttc
     1261 atacgtgaac gagatacgat tattattcct agtcttgatc gattagcaag aaatagtaaa
     1321 gatacaaaag acttacttca attgtttcgt agtaaaggtg cttctattga aatattagac
     1381 ctaccgtcgt ttaatggggt aactgaccct gcattaagag acttgctaac aaatttagtc
     1441 atagaagtgt ttagctatgt tgctgaaaac gaacggaaaa aaatcaaaga gcgtcaaaaa
     1501 caaggcattg aaattgcaaa gaaaaatggt cgttatactg gtaaaaagat tcaataccat
     1561 gccaatgcaa caggaaaaaa caaattgatt tacgatgaaa ttgttgatgg tctgaagaaa
     1621 aaagaaacta tttctaaaat agcaaagaac gcaggagtta caagaaaaac cgtttatcga
     1681 gtaaaaagag atttaggaat gtaatatgtt ccagttagaa a
//
LOCUS       DAJSBC010000187         1664 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSBC010000187 DAJSBC010000000
VERSION     DAJSBC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.197"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            131..505
                     /locus_tag="ONT65_002924"
     CDS             131..505
                     /locus_tag="ONT65_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2330473.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ONT65_002925"
     CDS             complement(619..1248)
                     /locus_tag="ONT65_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2330474.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ONT65_002926"
     tRNA            complement(1382..1454)
                     /locus_tag="ONT65_002926"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAJSBC010000188         1634 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSBC010000188 DAJSBC010000000
VERSION     DAJSBC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.198"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            101..868
                     /locus_tag="ONT65_002927"
     CDS             101..868
                     /locus_tag="ONT65_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2330475.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ONT65_002928"
     CDS             1060..1458
                     /locus_tag="ONT65_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR2330476.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DAJSBC010000189         1621 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSBC010000189 DAJSBC010000000
VERSION     DAJSBC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.199"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(28..1509)
                     /locus_tag="ONT65_002929"
     CDS             complement(28..1509)
                     /locus_tag="ONT65_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330477.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          509 a          224 c          285 g          603 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
     1621 g
//
LOCUS       DAJSBC010000190         1594 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSBC010000190 DAJSBC010000000
VERSION     DAJSBC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.200"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..279)
                     /locus_tag="ONT65_002930"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="ONT65_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="ONT65_002931"
     CDS             complement(545..739)
                     /locus_tag="ONT65_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330478.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="ONT65_002932"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ONT65_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          562 a          241 c          213 g          578 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agta
//
LOCUS       DAJSBC010000191         1578 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSBC010000191 DAJSBC010000000
VERSION     DAJSBC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.202"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..752
                     /locus_tag="ONT65_002933"
     CDS             138..752
                     /locus_tag="ONT65_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330479.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="ONT65_002934"
     CDS             complement(776..1126)
                     /locus_tag="ONT65_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2330480.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="ONT65_002935"
     CDS             complement(1120..1383)
                     /locus_tag="ONT65_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR2330481.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          521 a          286 c          245 g          526 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaa
//
LOCUS       DAJSBC010000192         1573 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSBC010000192 DAJSBC010000000
VERSION     DAJSBC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.203"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAJSBC010000193         1553 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSBC010000193 DAJSBC010000000
VERSION     DAJSBC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.204"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            40..1506
                     /locus_tag="ONT65_002936"
     CDS             40..1506
                     /locus_tag="ONT65_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR2330482.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          429 a          290 c          327 g          507 t
ORIGIN      
        1 taatattttc tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag
       61 ggcttcttcg ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga
      121 tttagttatt acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac
      181 gggggacttg gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg
      241 atctacatgt ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc
      301 actgtattct tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt
      361 ggtgtaagta cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa
      421 ccaaatgttt ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca
      481 ggattttcga ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt
      541 ggtacaatcg gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg
      601 ttctttggat tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca
      661 acaccgccga atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc
      721 gcacttgttg ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt
      781 gacttttttg tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc
      841 aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc
      901 ccattattcc ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta
      961 gctttatttg ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc
     1021 tggtatcaat cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg
     1081 tggacgaaac ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta
     1141 tttgcaggat tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga
     1201 aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt
     1261 ctttcaccag ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa
     1321 acaatcgcta tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca
     1381 cgcttctaca caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc
     1441 gtcggtggta tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt
     1501 cattaatcaa tgaacataaa aacaggaatt gcttatttgc agcagttcct gtt
//
LOCUS       DAJSBC010000194         1520 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSBC010000194 DAJSBC010000000
VERSION     DAJSBC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.205"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..848
                     /locus_tag="ONT65_002937"
     CDS             <1..848
                     /locus_tag="ONT65_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2330483.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1390..>1520
                     /locus_tag="ONT65_002938"
     CDS             1390..>1520
                     /locus_tag="ONT65_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2330484.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
BASE COUNT          502 a          267 c          295 g          456 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaattcggtt
     1321 ctgttgcaaa gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag
     1381 gacagagtaa tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct
     1441 gttggttact acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat
     1501 cgtggaataa atgtttgtca
//
LOCUS       DAJSBC010000195         1470 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSBC010000195 DAJSBC010000000
VERSION     DAJSBC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.206"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(251..406)
                     /locus_tag="ONT65_002939"
     CDS             complement(251..406)
                     /locus_tag="ONT65_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330485.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(865..1122)
                     /locus_tag="ONT65_002940"
     CDS             complement(865..1122)
                     /locus_tag="ONT65_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2330486.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1115..1384)
                     /locus_tag="ONT65_002941"
     CDS             complement(1115..1384)
                     /locus_tag="ONT65_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2330487.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          484 a          257 c          159 g          570 t
ORIGIN      
        1 aaggggatac tttcgtgtcc atgcatgtaa ttaactaatt gttgagcttc agaagttata
       61 tctctaatta tatagttaac ggaattagga agtgtcttta actctaatga aatttctatc
      121 caattactat cttctaatct gtatatgctc gaagtataat ttgattcgtc agcaagcatc
      181 gctacatccc aaacactgat cataaaatct tttaaacgtt caatcatttc agcaatattg
      241 ctaaaatcag ctaatttttc tttagaataa aaaaaacatt tatatcagaa tcaataaatt
      301 ctattttttt tattactata tcgtcatcat tatcttgacc atttatgttt attgttccct
      361 gagtggggag tacttctttc aaattcaagt taaatttata ttccattact tctcctccaa
      421 tatataaaaa ttacttaact gtatccaaat ttaatatgta aataaatcat taatttttag
      481 ctaaattcta tgagaagttt atttttgttg tttttgctaa ttaactaaaa ctagtacaaa
      541 aaatttaaaa tagattatta aacctattgc ttattctatt cagtcatata tataagcgtc
      601 atcctttagt aactaacatc ccacactaca atagtattat aacatatcta ttcaacttca
      661 aattgttgaa atttaagcca tttatattta cttttcctca actttagaaa tgattttact
      721 aaccttatat ccgtaatacg cgtaacaaac acattcatta ataacaatca acactaagct
      781 atattagctt tttaaatgac tagggtaaat aaaataagga tcaaaaactt ttaagtttct
      841 gaccctttcc ttacttccga ttggttaata gtgatctttt gcagaataaa taaatatcgt
      901 ttcattttca actctatata tcagtctatg ttcatctgta attcttctgg accattttcc
      961 agataaatca tgctttaatg gctcaggttt tcctaatcca gcaaaggggg aacgatcgat
     1021 atcttttatt aacttgttaa tcttttttat attgcttttg tttccttgct catgccaata
     1081 aagataatca tcccaagcat catcagacca agccttaatc atcagattca acctcgatta
     1141 agtcatgtgt tttaaatgca cctttggaga attgttcatc tcctcgacga attttttcca
     1201 tgacgtaatt attagaaagt gttctcaacg tttcttgcat agaatcataa tctctttttg
     1261 ataatacaac aactgtatct tctacatctt tacttgttac aataagtgtt tcagcatcct
     1321 cattaacttg tttcatataa ctacgtaaat tttggcggaa atttgaataa gctactgctt
     1381 ccattccttt caccccttat ttatttctat aaaacaattg tacattatat tgtacaatta
     1441 agcaaatgat taatattcta acttcttata
//
LOCUS       DAJSBC010000196         1457 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSBC010000196 DAJSBC010000000
VERSION     DAJSBC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.207"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..626)
                     /locus_tag="ONT65_002942"
     CDS             complement(<1..626)
                     /locus_tag="ONT65_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330488.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1457
                     /locus_tag="ONT65_002943"
     CDS             914..>1457
                     /locus_tag="ONT65_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2330489.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEE"
BASE COUNT          505 a          212 c          279 g          461 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaa
//
LOCUS       DAJSBC010000197         1453 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSBC010000197 DAJSBC010000000
VERSION     DAJSBC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.208"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..625)
                     /locus_tag="ONT65_002944"
     CDS             complement(<1..625)
                     /locus_tag="ONT65_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2330490.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ONT65_002945"
     CDS             complement(784..1050)
                     /locus_tag="ONT65_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2330491.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ONT65_002946"
     CDS             complement(1040..1396)
                     /locus_tag="ONT65_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2330492.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DAJSBC010000198         1451 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSBC010000198 DAJSBC010000000
VERSION     DAJSBC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.209"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            105..1406
                     /locus_tag="ONT65_002947"
     CDS             105..1406
                     /locus_tag="ONT65_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2330493.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DAJSBC010000199         1447 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSBC010000199 DAJSBC010000000
VERSION     DAJSBC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.210"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..307)
                     /locus_tag="ONT65_002948"
     CDS             complement(<1..307)
                     /locus_tag="ONT65_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330494.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     "
     gene            complement(309..1367)
                     /locus_tag="ONT65_002949"
     CDS             complement(309..1367)
                     /locus_tag="ONT65_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR2330495.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          562 a          240 c          173 g          472 t
ORIGIN      
        1 tttttgtgaa ataattttcc ccctgtataa aaatttcatc ataaacggat aaatcatccg
       61 agaaaaattg attatggtta gttaacaagt taattttacg tttaaaggaa gaaccgattt
      121 tccggtgatt cacaaaaaca acatcattaa atagccctaa ttcttttata cgctcactat
      181 atcttctagc gttttcaaat tcagaataaa taaataaatc tccttttgat tctcggtaaa
      241 aattttgata aacatttaat atattaatca aatgtaaagg agtccaagca atgaaagctg
      301 tcttcatatt aattctcctt ttttaaatac aagtaagaaa tgcttcccaa ataataccag
      361 aaaacaattg aaatgacatc gcgcttattg aaaagtacat gagcctccac catttcatct
      421 ataaaaatag atagtataaa aattaaaaca ataccaagaa acgcataatt cactttactt
      481 ctaaaaaatg ttttgagtaa actaaagata tttaacacat agaaagcata gatacatata
      541 aaagcaatta taccacttga tacaagtact tgaagaaatc cattatgcat gtttccatgt
      601 gctcttttta gttcattcac atctgtagta tcaagatcta cttttttcaa aaattttgca
      661 tcgctattcc catagaaatc ggtctctcca taaccaaata acaaagttgg tcttataact
      721 ttaagcgcag ctttccaaat cgtaaaacga ccagctgaaa tttcggagtt atcactagaa
      781 tgctgtaata ttacttcttt ttctggaagt ggagttttat ctgattcatt tgactctata
      841 acttctactt ttctatcaat cgatacagga attgttgcta aaaaatcctt agcataatta
      901 gtaccaaaag aaaaatatac caatagaact aataatatgc ttaccatcca tttgattttt
      961 acaggtagta cttctctaat gctctttcta aattttgaat tggcaaacaa caaaagaaat
     1021 aatactaaaa aagaacaaat tactacctgg gcacctctag acgaagaaag tgtaatataa
     1081 aatatttgta cgataaaatt catcatataa aaaacacgaa tcgtttttgt tatttttgaa
     1141 attaatacac taattaaact taaaataata gaagtatatc caaacatagc tccaacattt
     1201 ggacttgtat agacaccaaa taaccgatgt tcaataaaac cttgtcttgc aagtaatcct
     1261 ggcgttcctg ttgggacatg ataagaaatc aaaaaaataa attgatagat agatattagc
     1321 gcagctaaaa aaattatgat aatgaaaaca ttattaaaat acaccatttt tttctttttt
     1381 tcctcttcac tttgtgaaaa gtctgcagga aaaattacta tcaaagtaat cagcaaatat
     1441 ccaaaat
//
LOCUS       DAJSBC010000200         1441 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSBC010000200 DAJSBC010000000
VERSION     DAJSBC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.211"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            92..1147
                     /locus_tag="ONT65_002950"
     CDS             92..1147
                     /locus_tag="ONT65_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2330496.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT          584 a          135 c          272 g          450 t
ORIGIN      
        1 atcttttaaa aagcaaaaag atgataaagt tactttttat gggatgttgg tagaaaacaa
       61 attattataa atattagagg tataaaaagt tatgaaacac aaggtaagtg tgatagtacc
      121 aatttacaat gttgaaaaat atttaagaaa atgtgtggat agtttattga gtcagacatt
      181 aaaagagata gaaatcgtat tagttgatga tggttcgcca gatgcttcgg gggaaatagc
      241 agatgagtat caaaaaaaat attctaatgt aaaaacaata catagagaaa atggggggtt
      301 aggaccggca agaaatacag gaattgagaa tgcgacagga gaatatgtgg cttttcttga
      361 ttcagatgat tgggtagaaa gtgatatgta tgaaaaatta tacttagttg cgtccaaaga
      421 aaatgctgat atagtagtta gtggacattg tgattttgcg aatgacaaag ctatggtgaa
      481 aaagcaacat cctttgagtg ggaatgtata tgatacgcct aatagtatta aaaatgtacg
      541 aaaaaattta tttggacata gtccaaaaga tcaagaagta gaggccttcc cgatgtcagt
      601 atgtatgtct ttgtataata aaaatctgct agataaatat agactaagat ttgaaaaaat
      661 tttatctgaa gatacaattt ttaacttgaa tgcatatgat gtcgcaaata aaattgcttt
      721 tacagaatat acagattatt gttatagaaa agaagaacaa gagtctatta caaaaagttt
      781 atctccaaat aagcagacgg aatttaaaga atttttaata actctaaaag ctatagcatc
      841 gaaagaagat ttagaatgtc aaatcagagc aaaacgtatg gcaattgatt attgtaggct
      901 ttatgtgggc ttagttgatg atgcaaattt gggaattaaa gagaaaagaa agtatataaa
      961 aaaatttatt gaagatgagg gattaattca atgttggaag ggatatccta caaatacgct
     1021 accatttcaa caaaaatttt ttcataaaat gcttgaatgt aagtgttata caatattgtt
     1081 gttgttaagt agaattagaa agataattaa aaatatgacc agaaaaaaat tgtttataat
     1141 attataaact aaaagtatat caaataatag aaattaacaa gttacaaaaa aatattataa
     1201 agaataatat tttctaaaaa gtaaactata gtaatgtgga ttatcacctt ccaacagtat
     1261 ataaaatgag cgtaaaaacg gctatttttg ccttaacaac gatttttttg tatacaaata
     1321 cgatatgctc attaattatt ggaatttgta gtgctgttta aattaatgaa ggtgtaatta
     1381 tatgagtgat cattttgaaa aagtttgttg agaagatgtg cttctagtca tgtgatggga
     1441 g
//
LOCUS       DAJSBC010000201         1417 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSBC010000201 DAJSBC010000000
VERSION     DAJSBC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.212"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(150..1337)
                     /locus_tag="ONT65_002951"
     CDS             complement(150..1337)
                     /locus_tag="ONT65_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR2330497.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAJSBC010000202         1405 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSBC010000202 DAJSBC010000000
VERSION     DAJSBC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.213"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            73..234
                     /locus_tag="ONT65_002952"
     CDS             73..234
                     /locus_tag="ONT65_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR2330498.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>1405
                     /locus_tag="ONT65_002953"
     CDS             253..>1405
                     /locus_tag="ONT65_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2330499.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFM"
BASE COUNT          570 a          126 c          240 g          469 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgt
//
LOCUS       DAJSBC010000203         1383 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSBC010000203 DAJSBC010000000
VERSION     DAJSBC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.214"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..510
                     /gene="mscL"
                     /locus_tag="ONT65_002954"
     CDS             49..510
                     /gene="mscL"
                     /locus_tag="ONT65_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR2330500.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(570..1016)
                     /locus_tag="ONT65_002955"
     CDS             complement(570..1016)
                     /locus_tag="ONT65_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2330501.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(1109..1354)
                     /locus_tag="ONT65_002956"
     CDS             complement(1109..1354)
                     /locus_tag="ONT65_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR2330502.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT          405 a          292 c          251 g          435 t
ORIGIN      
        1 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
       61 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
      121 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
      181 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
      241 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
      301 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
      361 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
      421 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
      481 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaag aaagccgatt
      541 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
      601 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
      661 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
      721 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
      781 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
      841 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttctcc atcaatgtca
      901 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
      961 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
     1021 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
     1081 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
     1141 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
     1201 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
     1261 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
     1321 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
     1381 cca
//
LOCUS       DAJSBC010000204         1379 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSBC010000204 DAJSBC010000000
VERSION     DAJSBC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.216"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            77..1246
                     /locus_tag="ONT65_002957"
     CDS             77..1246
                     /locus_tag="ONT65_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HCR2330503.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          507 a          204 c          233 g          435 t
ORIGIN      
        1 gtgtaaatag aaaaaaggaa gtcccttctg tagaatagag ttaccacaac acattcacag
       61 aaaagaggac ttccatatga acgattttac tacagaaatt atggaaatac taatcaataa
      121 aggtgatttg gatgatttgt ttcgtcgtca cctagaattg gctattaacg ctttattaca
      181 agctgaatta acggtatttc ttgactatga aaagtatgat agagctggat ttaattcagg
      241 taattcccgt aatgggaatt attcgcgctc ttttaaaaca gagtacggtg aattgaattt
      301 agtaatacct agagatagga atggagactt ttctcaacaa actttacctg cttataaaag
      361 aaccaatgac tctttagaaa ccactattat tcagctagtt caaaaaggaa ttactatgtc
      421 cgaaatctct gatctaatcg aaaaaatgta tggtcattat tacaaaccac aaactatttc
      481 aaatatgagt aaaattgtgt ctgaagatgt ttcggcattt aaagaaagaa ctttagaagc
      541 taaatactca gtcattttta tggatgctac tcatattccc ttaaaaagac aaaccgtatc
      601 aaaagaagct gtctatatag taattggtat caggttggat ggaactaaag aggttctagg
      661 atttactata gctccaactg aatctgctta tgtttggaaa gagattcttc aagatctaaa
      721 agaccgtggt ttagaagagg ttttgttagt cgtaacagat ggtttaagtg gtattgacga
      781 tagtattcat agtgtttatc caaatgctca atttcaacaa tgttgtgtcc atatatctag
      841 aaatatcgct cataaggttc gtgttagtga tcgacaagaa atctgtagcg atttcaaatt
      901 agtttaccaa gcaagttcaa aagaagaagc aaataatcaa ataaggttta tgatagataa
      961 atggaaaaag cagtatccac gagtggttaa attgctcatg aatcctgcta tattaacctt
     1021 ttataatttc cctccgtcaa tcagaagaac tatttattca actaatttga ttgaaggttt
     1081 taacaaacaa ttaaagaaat ataccaaaag aaaagagcaa tttcctaatg aagaatctct
     1141 ggagagattt ctagtatctc aattcaacaa ctataatcaa aagtttctat gccgagttca
     1201 taaaggtttt aaagaaatac atgatacatt agaatcaatg ttttaacttg atcaacagaa
     1261 tgggcttttc catttacaca aaattattga cgctcccgac aaaattatga tcttgcgtaa
     1321 agttataaaa tattaataaa aatattttat taatgaaaag cttggtagca cggttattc
//
LOCUS       DAJSBC010000205         1375 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSBC010000205 DAJSBC010000000
VERSION     DAJSBC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.217"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            191..445
                     /locus_tag="ONT65_002958"
     CDS             191..445
                     /locus_tag="ONT65_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2330504.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ONT65_002959"
     CDS             446..763
                     /locus_tag="ONT65_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2330505.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DAJSBC010000206         1340 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSBC010000206 DAJSBC010000000
VERSION     DAJSBC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.219"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1310
                     /locus_tag="ONT65_002960"
     CDS             <1..1310
                     /locus_tag="ONT65_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HCR2330506.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          570 a          102 c          226 g          442 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttatggaat taaaaatatt aaacaggaat
     1261 ttatcgaaaa tggtagaaaa gaaatttata aaaggactta taaagattga ttatataata
     1321 tatgaaaagc tattataaaa
//
LOCUS       DAJSBC010000207         1309 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSBC010000207 DAJSBC010000000
VERSION     DAJSBC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.220"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            19..1143
                     /locus_tag="ONT65_002961"
     CDS             19..1143
                     /locus_tag="ONT65_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2330507.1"
                     /translation="MIVVVLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILT
                     TNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGS
                     VQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTI
                     PMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLG
                     LQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTL
                     FTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDS
                     QDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          457 a          244 c          219 g          389 t
ORIGIN      
        1 agaaaatatt catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca
       61 ggaactattc ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct
      121 caatcagctc aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc
      181 gatcaactat taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc
      241 atcatcatgg aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct
      301 tctctttatt atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa
      361 caagtcgagc gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt
      421 gctggcagcc agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga
      481 actgcttctc caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac
      541 ttaaaattta cagattatca gacctctcat tatgattcat tgaactttac catattgact
      601 ggaattttga tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc
      661 gaacctttag gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat
      721 gcactatcct atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa
      781 acagagatcc ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat
      841 acgctgttta cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta
      901 tggcgtgaaa atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat
      961 atcctgtcgc aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca
     1021 caggatgcta tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact
     1081 gctgatctaa gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc
     1141 tgatatgatg aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta
     1201 ctcgactaaa cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact
     1261 ttaattccaa ataaacagaa cagaaagagg tgttcttctt tctgttctg
//
LOCUS       DAJSBC010000208         1281 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSBC010000208 DAJSBC010000000
VERSION     DAJSBC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.221"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..963
                     /locus_tag="ONT65_002962"
     CDS             4..963
                     /locus_tag="ONT65_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR2330508.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ONT65_002963"
     tRNA            1152..1225
                     /locus_tag="ONT65_002963"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          426 a          245 c          258 g          352 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca a
//
LOCUS       DAJSBC010000209         1278 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSBC010000209 DAJSBC010000000
VERSION     DAJSBC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.222"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(305..661)
                     /locus_tag="ONT65_002964"
     CDS             complement(305..661)
                     /locus_tag="ONT65_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR2330509.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="ONT65_002965"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="ONT65_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2330510.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          415 a          229 c          179 g          455 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaa
//
LOCUS       DAJSBC010000210         1270 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSBC010000210 DAJSBC010000000
VERSION     DAJSBC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.223"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..383
                     /locus_tag="ONT65_002966"
     CDS             <1..383
                     /locus_tag="ONT65_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2330511.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="ONT65_002967"
                     /pseudo
     CDS             complement(367..960)
                     /locus_tag="ONT65_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          392 a          250 c          215 g          413 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaatc
     1201 acacctataa atttattact atataaggga tacttaaacc aaatacccca tctaattatt
     1261 ttataattta
//
LOCUS       DAJSBC010000211         1214 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSBC010000211 DAJSBC010000000
VERSION     DAJSBC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.224"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            70..1026
                     /locus_tag="ONT65_002968"
     CDS             70..1026
                     /locus_tag="ONT65_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330512.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="ONT65_002969"
     CDS             complement(1080..>1214)
                     /locus_tag="ONT65_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2330513.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAJSBC010000212         1195 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSBC010000212 DAJSBC010000000
VERSION     DAJSBC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.225"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1076
                     /locus_tag="ONT65_002970"
     CDS             <1..1076
                     /locus_tag="ONT65_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2330514.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAJSBC010000213         1193 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSBC010000213 DAJSBC010000000
VERSION     DAJSBC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.226"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>1193)
                     /locus_tag="ONT65_002971"
     CDS             complement(<1..>1193)
                     /locus_tag="ONT65_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2330515.1"
                     /translation="DKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRII
                     RNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAI
                     LMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVS
                     GSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLK
                     TVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKD
                     STTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQ
                     ETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGY
                     RNFYN"
BASE COUNT          340 a          229 c          248 g          376 t
ORIGIN      
        1 gttataaaag ttgcgataac caaacgcaat tcgtttaatc agcttgatct tgttattcag
       61 cccctcagtg acgccgtttg agtgggacac cttaaaggca tttgaaatac ccgattggta
      121 gcaatggaag atcgtgaatt tctttttaaa cgactgaggt aacgtagatg aaattgtttt
      181 aagggtttct tgaaagcctt ccgtacctcg tttaacgaaa taatagcgta ataattgaca
      241 aaaatgatac gcagttcgca attcttcgtt gtagcttaac agctcatcaa cgatttctgt
      301 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagtggct cccatgtcgt
      361 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
      421 agagtgtgag gttcgaaaac gattcatgat ctggacacgt aattgattga agctgcgatt
      481 gatgtgttgg acaatatgaa agcggtcagt tacgatctcc gcatttggaa agactgtttt
      541 aagcaactga caatagctgg cattcatgtc catcactagg tacttgacct ttaaacgggc
      601 ttttctagga aagtgcagga agtattgagt tagtttgtaa agtctgcgat cttcaaggat
      661 cgtaaatagc tgatgggttg tcccatcaac acaaataaaa ctcatggaac cagaaacaga
      721 cttcattgat ttgaactcat ccatacacaa caccgtcgga aggaagttca tacgtgactg
      781 gtaatgcttc gttgcttcac gcaagatccg tgtcaccgtc ccatccgaga cgaagtactt
      841 cttagcgatt agtttacgtg attgattttc gtacagttcc atgagaattg cctgcttcag
      901 ttcctttgtt agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtccttcc
      961 acaatcatgg cagagaaaac gggtacgttt taattctaaa atagttaact tttctttgac
     1021 tttcaatatc tgagtgcgtg tcgtatagga accgttgcgg ataatacgaa ccgatcgaca
     1081 gtgttcacaa taggaaggag agtcatccaa ttttccttta ataaactgcg cttgaacgcc
     1141 tttatattct ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctg
//
LOCUS       DAJSBC010000214         1176 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSBC010000214 DAJSBC010000000
VERSION     DAJSBC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.227"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>1176
                     /locus_tag="ONT65_002972"
     CDS             <1..>1176
                     /locus_tag="ONT65_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR2330516.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVL"
BASE COUNT          385 a          208 c          257 g          326 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tacttt
//
LOCUS       DAJSBC010000215         1159 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSBC010000215 DAJSBC010000000
VERSION     DAJSBC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.228"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..969
                     /locus_tag="ONT65_002973"
     CDS             <1..969
                     /locus_tag="ONT65_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2330517.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          393 a          204 c          232 g          330 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccag
//
LOCUS       DAJSBC010000216         1156 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSBC010000216 DAJSBC010000000
VERSION     DAJSBC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.229"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..104
                     /locus_tag="ONT65_002974"
     CDS             <1..104
                     /locus_tag="ONT65_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2330518.1"
                     /translation="ETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
     gene            166..783
                     /locus_tag="ONT65_002975"
     CDS             166..783
                     /locus_tag="ONT65_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330519.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          416 a          156 c          203 g          381 t
ORIGIN      
        1 tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc ttcggctttt
       61 cggtgtctac tgaaatcaag gtattaatgg gaataacagc ctaagatatt tggagttcag
      121 agagggcgcg tttgattttc aaacttcgca acagaaccga gaaccttggg ttttcaagtg
      181 ctttttggtt cattaatctt aaccatagtg tttttaaagt ggaaaacaag caacaaaaaa
      241 agtatgtgga gccttgtagg aatagttctt attattttta ctgtgactgc agcaacagaa
      301 gcaaattatt cgcataaaat tcagtcgtat aaaggtcgtt tagttatgac caaaaaagat
      361 gttacacgtt atacatcctc aagaactgga ggcctatatg actatgcacc attagatgca
      421 ttgaaatata aagatcattt aaagaaacat gaagtaaaag ttggtgatcg gtgggagaat
      481 gcatctcata aagcatataa ttccaaaata gtctatacag tagaagatgc cgatgagcaa
      541 aatatcacat tacctgtatt caattattgg ggaacagttg tgaaagtaaa tgggaaaaag
      601 agcactatag aaaatgacaa tggtttagtg tctattaaag gtacggggaa aagaactata
      661 gttgaggttt catctacgta tccaactacg atgattctat ctttattaat tagttcaata
      721 acttttatat tcttatttac acgttattta ataagatgct gcggtgaaat gaaaatagat
      781 tagatagata ttataaatat gttgattcta ttttcatagc aatggagaaa atacattagc
      841 aatatataaa agaaggtatc aagagtcaca gtgaatttat tccgaaaagt gaaaagagca
      901 aatataaaat ttaatgaaaa tatagaacta aataggaaaa cgtagacgta attttctacg
      961 ttttcctttt catatatatt aaatattacg gaaaattata taaactaatt tagtgttttg
     1021 tttgtaattc ctaattttgc tccactttta gataattttt aggtctattt aactcatcag
     1081 acattctatg ctcatttttc tccaaatttg tttcaactct ttcgtactta attttgcgta
     1141 tttgaaaatt cttctt
//
LOCUS       DAJSBC010000217         1143 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSBC010000217 DAJSBC010000000
VERSION     DAJSBC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.230"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT65_002976"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT65_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR2330520.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAJSBC010000218         1051 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSBC010000218 DAJSBC010000000
VERSION     DAJSBC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.232"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ONT65_002977"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ONT65_002977"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR2330521.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAJSBC010000219         1011 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSBC010000219 DAJSBC010000000
VERSION     DAJSBC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.233"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..>1011
                     /locus_tag="ONT65_002978"
     CDS             12..>1011
                     /locus_tag="ONT65_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR2330522.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEA"
BASE COUNT          332 a          188 c          202 g          289 t
ORIGIN      
        1 cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag aaagatttga
       61 tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc gtcgatgctg
      121 ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta gatggaaacg
      181 ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt gaacaataca
      241 atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa aactgtcgaa
      301 cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg aatcatgtca
      361 gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaaatt
      421 gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc tacttaccaa
      481 agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
      541 ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa ttaattgatg
      601 tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc gctaatcctg
      661 aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt accaaacgta
      721 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac atgaatcaag
      781 cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga cagaaaagtc
      841 aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc
      901 attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt cttacggaag
      961 cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg t
//
LOCUS       DAJSBC010000220         1003 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSBC010000220 DAJSBC010000000
VERSION     DAJSBC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.234"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            174..>1003
                     /locus_tag="ONT65_002979"
     CDS             174..>1003
                     /locus_tag="ONT65_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2330523.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          352 a          145 c          170 g          336 t
ORIGIN      
        1 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
       61 aaaatgatag cgtaaaatat tttgtgtaaa tagatattta gggtagaaaa aaagaaagtc
      121 cattctgtaa aattaaagtt accacaaccg aaattttata ggaggacttt ctcatgccca
      181 attttactac agaaattatg gaaacactaa tcaataaagg tgatttagat gaattatttc
      241 gatgtcattt agaattagct gttaattcat tattgcaggc tgaattaaca gcttttcttg
      301 attatgaaaa atatgatcgt gctgggttca attcaggtaa ttcccgtaat gggaattatt
      361 cacgttcatt cagaacagag tatggagaat taaatttggt aattcctaga gatagaaatg
      421 gtaagttttc tcaacaaact ttacctgctt ataaaagaac caatgactct ttagaaacca
      481 ctattattca gctatttcaa aaaggaatta ctatgtctga aatctctgaa ttgattgaaa
      541 aaatgtacgg gcatcactat acgccacaaa ccatttcaaa tatgactaaa atagtttctg
      601 aagatattat tgcctttaaa gaaaggtcct tagaatccag atactctgtc atttttatgg
      661 atgctactca cattccttta aaaagacaaa ctgtttcaaa agaagcagtt tatattgtaa
      721 ttggcattcg attagatgga accaaagagg ttcttgggtt tagtcttgct ccaactgaat
      781 ctgcttatgt ttggaaagag attcttcaag acctaaaaga ccgtggttta aaagaggttt
      841 tattagtcgt aactgatggt ttaagtggca ttaacgatag tattcatagt gtttatccaa
      901 atgctcaatt tcaacaatgt tgtgtccata tatctagaaa tatcgctcat aaggttcgtg
      961 ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt tta
//
LOCUS       DAJSBC010000221          995 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSBC010000221 DAJSBC010000000
VERSION     DAJSBC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.235"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..913)
                     /locus_tag="ONT65_002980"
     CDS             complement(26..913)
                     /locus_tag="ONT65_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2330524.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAJSBC010000222          994 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSBC010000222 DAJSBC010000000
VERSION     DAJSBC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.236"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>994)
                     /locus_tag="ONT65_002981"
     rRNA            complement(<1..>994)
                     /locus_tag="ONT65_002981"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAJSBC010000223          988 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSBC010000223 DAJSBC010000000
VERSION     DAJSBC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.237"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..704
                     /locus_tag="ONT65_002982"
     CDS             <1..704
                     /locus_tag="ONT65_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2330525.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DAJSBC010000224          978 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSBC010000224 DAJSBC010000000
VERSION     DAJSBC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.238"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>978
                     /locus_tag="ONT65_002983"
     CDS             82..>978
                     /locus_tag="ONT65_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR2330526.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYE"
BASE COUNT          333 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaa
//
LOCUS       DAJSBC010000225          959 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSBC010000225 DAJSBC010000000
VERSION     DAJSBC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.239"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(107..511)
                     /locus_tag="ONT65_002984"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ONT65_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ONT65_002985"
     CDS             complement(570..>959)
                     /locus_tag="ONT65_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330527.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAJSBC010000226          958 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSBC010000226 DAJSBC010000000
VERSION     DAJSBC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.240"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            324..958
                     /locus_tag="ONT65_002986"
                     /pseudo
     CDS             324..958
                     /locus_tag="ONT65_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          324 a          181 c          177 g          276 t
ORIGIN      
        1 ccagaaagaa atgaacacct tcaattatct aaatctcgaa aatggttaga tgtaatcatc
       61 aggaaaaaca atcttaaacg aattactatt catggtctta gacacacaca tgctagcttg
      121 cttttagcaa gctagtgcaa atattaaaga tgtgcaagag cgtttaggcc actcttctat
      181 tcaaatcatt atggatctat atattcatat tacagacaaa agaaaagaag aaaccgtagc
      241 gcaattcgca aaatataggc tctataattt atgggactga tgaatcaaaa ttgattcttc
      301 agtcccattg ttattttagg tatatgattc gtacattgaa agagttgaaa aactatcttc
      361 cgaagccgtc aaaaaggacc ctgccaaaag tattgatggt ggatgaattt cgttcgcacg
      421 cttcaattgt ggataaaatg agctttattt gtgcggatgg cgagactgga aaactcattg
      481 atattttgcc tacacgaaaa ttgtctcgat taacacacta ttttagtcgc tgtgcaaatc
      541 ctgaagcggt ggaatattta gtgacagata tgaacgccgc ctacttccaa ctgacaaaac
      601 gtgtcttacc aaatgcgaaa gtcgtcattg attgttttca tatcatcaaa catttgaatc
      661 aggcgttcaa tgattttcgt gtacaagaaa tgaaaagatt acaccagtta gggcaacgac
      721 gggaagccga gaaagtgaaa aagaactggc gctttcttct aaaaaaccgt caaaaaatca
      781 atcactatga ttacaaaaca tggcgcagtt ttcgtgcacc taaatacccg ctactcactg
      841 aagcaatgat gattgatcga ttgctggcat tttcaacgcc tttaaaggac gcctatactt
      901 ttttccatga gctgaccgaa gcctttagag acaaggaatc agacttattt tttacctt
//
LOCUS       DAJSBC010000227          920 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSBC010000227 DAJSBC010000000
VERSION     DAJSBC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.241"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            178..870
                     /locus_tag="ONT65_002987"
     CDS             178..870
                     /locus_tag="ONT65_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2330528.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          429 a           98 c          138 g          255 t
ORIGIN      
        1 taaaaaaata ttgatacata aataaattag ttgtagtaaa tagttttaga attgaatttc
       61 tctatccttt ttaaataatt ttttagacaa aaaacagaaa gtgtctaaag aaagcttgac
      121 gaagaaaata atttttaata acttatttaa ggaaattttt tgaaggagaa gataaaagtg
      181 agaaaaaaga ttattattaa tgctggagta attataggat tgatgttagt aattttctcc
      241 ggaaagcaat atgctgataa tattttatta tggggagggg aagataatat tgaaaagatc
      301 aacaacaatt taactatatt agacacagct ctagaaaata aagagcaaaa aattagtatt
      361 ttaaattcac aattgtcttc aaacaaccaa actcttgaac aactaagaac taatattgaa
      421 gtttccaaac aaaaagtggc atctttagaa aacgacaaga atcaattagt atcagagaaa
      481 actaatttag aaaatcaatt gtcagcaaaa gactctgaat tgcaaggaaa acaaaatgaa
      541 attaatgcga aaatagacga aattaaccaa aaagtagctg aaataaatcg attaaatagc
      601 gattggaata atcgtttaca aaatgaaaag aacaaattta atgaagaaca atctactatt
      661 aatagtttaa accagcaaat taatcaaatg ttgagtgaaa aaaataacat tcaaaatcaa
      721 ctgcaagcta ctcagcaaaa gctagaagaa gaaaagtcaa aaaatagtag tttgaataat
      781 tacattgaaa agcttcaaaa agctaaaaat gatgtagagg ataccgcaaa taaagcacaa
      841 caaattgtaa atgaacatac aatccaatag atatagtttt aatttaacaa atggtatgtt
      901 tggagataat gagatagaat
//
LOCUS       DAJSBC010000228          907 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSBC010000228 DAJSBC010000000
VERSION     DAJSBC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.243"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..888
                     /locus_tag="ONT65_002988"
     CDS             <1..888
                     /locus_tag="ONT65_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR2330529.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          119 c          149 g          250 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcag
//
LOCUS       DAJSBC010000229          852 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSBC010000229 DAJSBC010000000
VERSION     DAJSBC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.244"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>852
                     /locus_tag="ONT65_002989"
     rRNA            <1..>852
                     /locus_tag="ONT65_002989"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          260 a          169 c          251 g          172 t
ORIGIN      
        1 tggcactagg agccgatgaa ggacgggact aacaccgata tgctttgggg agctgtacgt
       61 aagctatgat ccagagattt ccgaatgggg gaacccaaca tctttaatag gatgttacga
      121 ttgtgtgaat acatagcaca ttcgaggtag acgcagagaa ctgaaacatc taagtacctg
      181 caggaagaga aagaaaattc gattccctga gtagcggcga gcgaaacggg aaaagcccaa
      241 accagcaagc ttgcttgttg gggttgtagg actccaatat ggtagttctt tcagatagtc
      301 gaatgacttg gaaaagtcag tcaaagaggg taaaaacccc gtagacgaaa tgtggaagac
      361 acctaggagg atcctgagta cggcggaaca cgagaaattc cgtcggaatc cgggaggacc
      421 atctcccaag gctaaatact ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg
      481 tgaaaagcac cccggaaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtc
      541 aaagcccgtt aatgggtgat ggcgtgcctt ttgtagaatg aaccggcgag ttacgattgc
      601 atgcgaggtt aagttgaaga gacggagccg cagcgaaagc gagtctgaat agggcgtttg
      661 agtatgtagt cgtagacccg aaaccatgtg atctacccat gtccaggttg aaggtgcggt
      721 aaaacgcact ggaggaccga acccacgtac gttgaaaagt gcggggatga ggtgtgggta
      781 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
      841 agcctcggaa tt
//
LOCUS       DAJSBC010000230          823 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSBC010000230 DAJSBC010000000
VERSION     DAJSBC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.245"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(133..717)
                     /locus_tag="ONT65_002990"
     CDS             complement(133..717)
                     /locus_tag="ONT65_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2330530.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          268 a          173 c          114 g          268 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttt
//
LOCUS       DAJSBC010000231          815 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSBC010000231 DAJSBC010000000
VERSION     DAJSBC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.246"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            533..>815
                     /locus_tag="ONT65_002991"
     CDS             533..>815
                     /locus_tag="ONT65_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR2330531.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
BASE COUNT          270 a          130 c          172 g          243 t
ORIGIN      
        1 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcgtaa attagagcgt
       61 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
      121 tgccgaggat aaggagttga cggctaaacc actaatccct gaagctgaca tttgctaaaa
      181 aatggcaagt ccattttttg gccaacctag cgttagcgaa gtttgggggc gcccttttca
      241 aagggtgcgg ttttattttt tggatatttg catagctaaa gagaagtgct attattgagt
      301 tatcaaataa ataaactgaa aaaagagcaa aaaaataaag tcgagcaacg ctggacacgt
      361 tctcgacctt tacggaaact aatcgcacta gctccgtaac ttcttaatca ttatacgaat
      421 atttttagat ttcttcaaga ctagagattt tttggtaatg aatttttaag agttacgtga
      481 acttttgaaa attctttcca gaagtctctt tttctttgag gaggaacaaa atttgagtaa
      541 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
      601 gtatcaaagt ttgacccttc ccttaaaaat gcagtggtca attgaccgaa ttaccatagt
      661 cggaaaactg aaagaaaata tttattatca cactccaaat gatgtgttga ttttaaattt
      721 tgaacagctt atgcgcctaa atgaaggtaa tggttattta agatctgtta gtaataatgg
      781 ttggcagttg ttagaccaac atgaagagaa tattg
//
LOCUS       DAJSBC010000232          813 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSBC010000232 DAJSBC010000000
VERSION     DAJSBC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.247"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            389..787
                     /gene="spxA"
                     /locus_tag="ONT65_002992"
     CDS             389..787
                     /gene="spxA"
                     /locus_tag="ONT65_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2330532.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          310 a          110 c          143 g          250 t
ORIGIN      
        1 taacagagaa aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt
       61 actattaaag aaataaaaaa cataaaaaat cttaagaaat cggtttcatt tagcttttga
      121 gataaaataa tagtacagtt ttatacgctg tttcatatgt ttagaaaatt tgaattagat
      181 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
      241 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
      301 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
      361 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
      421 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
      481 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
      541 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
      601 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
      661 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
      721 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
      781 attatagaag gataatacga aacttggaaa aga
//
LOCUS       DAJSBC010000233          811 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSBC010000233 DAJSBC010000000
VERSION     DAJSBC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.248"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..672
                     /locus_tag="ONT65_002993"
     CDS             <1..672
                     /locus_tag="ONT65_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330533.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAJSBC010000234          765 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSBC010000234 DAJSBC010000000
VERSION     DAJSBC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.249"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..276)
                     /locus_tag="ONT65_002994"
     CDS             complement(<1..276)
                     /locus_tag="ONT65_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330534.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          258 a          129 c          129 g          249 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaagcttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaa
//
LOCUS       DAJSBC010000235          754 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSBC010000235 DAJSBC010000000
VERSION     DAJSBC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.250"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..529
                     /locus_tag="ONT65_002995"
     CDS             <1..529
                     /locus_tag="ONT65_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330535.1"
                     /translation="QIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGE
                     PVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFN
                     DKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKN
                     ILKNVKIAESLDDIL"
     gene            526..>754
                     /locus_tag="ONT65_002996"
     CDS             526..>754
                     /locus_tag="ONT65_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2330536.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKN"
BASE COUNT          289 a           94 c          128 g          243 t
ORIGIN      
        1 acaaattcca ttagttacag ctgggtcaag aaccgcattg caactttcgg atatttttaa
       61 ccctatgata gatatttata aagaaaaaaa atatatattt tttacgtctg tttatgattt
      121 tgagggcgga gaacctgtcg gagaatatga attagtatgc aaaatagcag acttagtagg
      181 aaaagataat ctaattgtta agacgcatcc tagagatgta agaacaattt ataaagataa
      241 tggatttatc gaggataaaa attcatcaat accttgggag gcgattcaat tgtcaggcga
      301 ttttaacgat aaaatattta tgacaattaa ttcaggaagt gttatttcag gtagtacaat
      361 gtctgcaaaa cctgtacgca cgtattatat gtataaattg tgtaacatag aaggaaatcc
      421 gtcttgcaaa aagaatgcgt atgatattga gcgattgctt aagcaaaaaa cgatgaaaaa
      481 cattttaaag aatgtgaaaa tagcagaaag cttagatgat atactatgag caaaactaaa
      541 tatttaatta aaaatatggg aatattaaca ataagtaatt tttcttcaaa aattttagtt
      601 tttttgttag tccctttata tacaagtgta ttatccacaa cagaatatgg cacatatgat
      661 ttgacaattt caacagccac attattgttt ccaattttaa ccttaaacat tgtagatgct
      721 gtgatgcgct ttttaatgga taaagataaa aata
//
LOCUS       DAJSBC010000236          746 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSBC010000236 DAJSBC010000000
VERSION     DAJSBC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.251"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            490..>746
                     /locus_tag="ONT65_002997"
     CDS             490..>746
                     /locus_tag="ONT65_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330537.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNY"
BASE COUNT          295 a           85 c          133 g          233 t
ORIGIN      
        1 ggtgaaagtt taatcctaat actgattttg tgtttaattg ttcatgaagt gaataaacaa
       61 ttgtgaatta aaaaaataaa aacctagtat aatcacttta ataaattgtt ggtagaaggt
      121 gatcaaaatg ggagtaaacg aaatgatgaa agtcagattg agtttttaaa tgataccttc
      181 ctcagaactt caaaacgaat tcaaagattc gttgacaaat cttgagcaag atcatttaaa
      241 aaaatatttt ctcttcattt gacgaaaaat gtttagaaat cttatataag tgaagataaa
      301 gtaattggtg taattaattg tagagaatag cctttgagca attgaagcga tacaattatt
      361 tagaaaatga acttcctcca gcatactaca tgattcctta cgggacgatg ttaaagaaga
      421 aagcaagaaa ttttagaagt attagtagat aaactaatgg gcttttctca atttaaggga
      481 gtagcccaaa tgtttatcaa atatggtgca gatacagtag caaccatgtt tacagaagaa
      541 tatcaagtag actattatga agtaatagat aaatgtgtac attttgttta tgaaaaagtg
      601 tggaatttta tgaattttgc gataagtgaa gaaatcttac gagtacaaga aaacagaaca
      661 ggaatattta ccagagacag aaagcttaat ttgattgcgc attatatacg gaatcgctgg
      721 tcatcaatac caaattttaa ttataa
//
LOCUS       DAJSBC010000237          729 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSBC010000237 DAJSBC010000000
VERSION     DAJSBC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.252"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..729
                     /locus_tag="ONT65_002998"
     rRNA            <1..729
                     /locus_tag="ONT65_002998"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          180 a          162 c          229 g          158 t
ORIGIN      
        1 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
       61 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      121 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      181 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      241 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      301 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      361 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      421 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      481 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      541 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      601 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      661 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      721 gaggactta
//
LOCUS       DAJSBC010000238          712 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSBC010000238 DAJSBC010000000
VERSION     DAJSBC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.253"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..446)
                     /locus_tag="ONT65_002999"
     CDS             complement(<1..446)
                     /locus_tag="ONT65_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330538.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAJSBC010000239          699 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSBC010000239 DAJSBC010000000
VERSION     DAJSBC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.254"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            132..344
                     /locus_tag="ONT65_003000"
     CDS             132..344
                     /locus_tag="ONT65_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330539.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>699
                     /locus_tag="ONT65_003001"
     CDS             506..>699
                     /locus_tag="ONT65_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR2330540.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDI"
BASE COUNT          249 a          104 c          122 g          224 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatt
//
LOCUS       DAJSBC010000240          696 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSBC010000240 DAJSBC010000000
VERSION     DAJSBC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.255"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(54..629)
                     /locus_tag="ONT65_003002"
     CDS             complement(54..629)
                     /locus_tag="ONT65_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2330541.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAJSBC010000241          685 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSBC010000241 DAJSBC010000000
VERSION     DAJSBC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.256"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(242..391)
                     /locus_tag="ONT65_003003"
     CDS             complement(242..391)
                     /locus_tag="ONT65_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330542.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          232 a          109 c          100 g          244 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgt
//
LOCUS       DAJSBC010000242          667 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSBC010000242 DAJSBC010000000
VERSION     DAJSBC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.257"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>667
                     /locus_tag="ONT65_003004"
     CDS             <1..>667
                     /locus_tag="ONT65_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2330543.1"
                     /translation="QQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAF
                     PISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFS
                     NEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKN
                     LFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMD
                     FNSV"
BASE COUNT          224 a          114 c          136 g          193 t
ORIGIN      
        1 caacagtata agaaaaacaa atcaaactgt ggtagacgtc cgcttgtttt acccgaggaa
       61 caatcagaat atattcaaag aaaggttgtt caaggatgga cacccgatgt gattgttggt
      121 cgtgcagcgt ttcctattag ttgttctgct cgtaccattt atcgtatgtt caaaaagggg
      181 ctatttgatt cttctgactt accgatgaaa ggcaagcgta aaccgaatgg acatcaagaa
      241 agacgtggaa aacaaacttt ccgccgctct attcatgaac gtgaaaagga ttatagccaa
      301 ttctcaaatg agtttggtca ccttgaaggt gacactatcg taggtctgaa acataaaagt
      361 gctgtaatta ccttagttga acgattatca aaagttatca tcacattgaa accgtgtggt
      421 agacaagcga ttgatattga aaaaaaatta aatcaatggt ttgaatctgt accgaaaaac
      481 ctattcaaat ccatcacttt tgattgtgga aaggaatttt caaattggaa acagatcagt
      541 aatgtcaatg atattgccat ttatttcgct gatccaggaa cgccgtctca aagaggccta
      601 aacgagaatt ctaacggatt gttacgtaga gatggtttat tgaaatctat ggatttcaat
      661 tcagtag
//
LOCUS       DAJSBC010000243          635 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSBC010000243 DAJSBC010000000
VERSION     DAJSBC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.258"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..270)
                     /locus_tag="ONT65_003005"
     CDS             complement(<1..270)
                     /locus_tag="ONT65_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330544.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ONT65_003006"
     CDS             complement(276..>635)
                     /locus_tag="ONT65_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2330545.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAJSBC010000244          630 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSBC010000244 DAJSBC010000000
VERSION     DAJSBC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.259"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>630)
                     /locus_tag="ONT65_003007"
     CDS             complement(<1..>630)
                     /locus_tag="ONT65_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330546.1"
                     /translation="KHKIKIIVSLILIFMGLKRIELLALSGAILVYYVLLKWGKKIQI
                     RALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGYV
                     GTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKP
                     IRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMIFLLVPMIVS"
BASE COUNT          249 a          117 c           67 g          197 t
ORIGIN      
        1 cacgaaacta tcataggtac taagagaaaa atcatttgag tgtctggata agtgagcgta
       61 ttatccgtca aatataatat aaacatatat atagttaata ataatacaca ctcccctgtt
      121 ttcaatccaa attttttagt tagtcttatc ggtttcacaa aaaataacca aaacatccat
      181 ataataaaaa ctatacatcc aacttctata taaatagtta atatatcact gtgtaggctt
      241 gctgcaattc ttccaccatt taacctaaag tgcgataaat aaagattaaa aaagtatttt
      301 gaaaaccatg tccatcctgt tcctacatat cctaaaccaa gttcataaaa tttagatgaa
      361 tatagataaa atgaaagcct cgcaccgtca tctgtaatgc ccaaatttga cataagcaat
      421 gccaaaattc ctgtatctat aatgtaaata aacactaacg aaataacagt tgcacaaata
      481 gtacacacta atgcacggat ttgaatcttt tttccccact ttaataaaac ataatacact
      541 agaattgcac cacttaaagc taacagttca attctcttca atcccatgaa aattaaaatt
      601 aaagatacga taatttttat cttatgtttt
//
LOCUS       DAJSBC010000245          583 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSBC010000245 DAJSBC010000000
VERSION     DAJSBC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.260"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>583
                     /locus_tag="ONT65_003008"
     rRNA            <1..>583
                     /locus_tag="ONT65_003008"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          125 c          169 g          117 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gta
//
LOCUS       DAJSBC010000246          580 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSBC010000246 DAJSBC010000000
VERSION     DAJSBC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.261"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            225..>580
                     /locus_tag="ONT65_003009"
     CDS             225..>580
                     /locus_tag="ONT65_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330547.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLS"
BASE COUNT          178 a           88 c           78 g          236 t
ORIGIN      
        1 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
       61 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      121 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      181 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      241 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      301 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      361 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      421 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      481 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      541 ttaaattaat aaaagctgct atagttactt tacttctttc
//
LOCUS       DAJSBC010000247          549 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSBC010000247 DAJSBC010000000
VERSION     DAJSBC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.263"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          246 a           76 c           90 g          137 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactct cgatgaagta gacctttgaa aactgaacaa
      541 agtaagaca
//
LOCUS       DAJSBC010000248          526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJSBC010000248 DAJSBC010000000
VERSION     DAJSBC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.264"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT65_003010"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT65_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR2330548.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="ONT65_003011"
     CDS             complement(349..>526)
                     /locus_tag="ONT65_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330549.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAJSBC010000249          524 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJSBC010000249 DAJSBC010000000
VERSION     DAJSBC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.265"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..524
                     /locus_tag="ONT65_003012"
                     /pseudo
     CDS             1..524
                     /locus_tag="ONT65_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          218 a           91 c           95 g          120 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aaca
//
LOCUS       DAJSBC010000250          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSBC010000250 DAJSBC010000000
VERSION     DAJSBC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.266"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..132)
                     /locus_tag="ONT65_003013"
     CDS             complement(<1..132)
                     /locus_tag="ONT65_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2330550.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAJSBC010000251          498 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSBC010000251 DAJSBC010000000
VERSION     DAJSBC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.268"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            178..381
                     /locus_tag="ONT65_003014"
     CDS             178..381
                     /locus_tag="ONT65_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HCR2330551.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..>498
                     /locus_tag="ONT65_003015"
     CDS             381..>498
                     /locus_tag="ONT65_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2330552.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQ"
BASE COUNT          191 a           56 c           89 g          162 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcagg
//
LOCUS       DAJSBC010000252          488 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSBC010000252 DAJSBC010000000
VERSION     DAJSBC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.269"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            99..293
                     /locus_tag="ONT65_003016"
     CDS             99..293
                     /locus_tag="ONT65_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330553.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          205 a           63 c           83 g          137 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtc
//
LOCUS       DAJSBC010000253          471 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSBC010000253 DAJSBC010000000
VERSION     DAJSBC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.270"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          162 a           74 c           96 g          139 t
ORIGIN      
        1 atatttttga aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg
       61 aagaggtttg gacaaaaatg gaaatttccg ccaaaagtaa ctgctcctca actgaaatca
      121 attaaagaac tagaatttga gaatgcactg gtaatttgta tgacatgtac tgatgaagta
      181 tttcgaaaac atttttaaag gttgagatgt atatagattt aaccaatcat ttatctctct
      241 cggaaaagga atattaacta accagcgggt tatcaaaaat aggtaggaaa actgtactta
      301 atggcttatt tcgcgctgat tatgagaata tcagtctgtt tgattaattt ttagggttca
      361 aaatacagag acaaactcca ggtcgggttt cgccacaagc gaattacccg atctggagtt
      421 ttgtctattg caggaaggcg tagccggact gcaggaataa agcccccggt t
//
LOCUS       DAJSBC010000254          462 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSBC010000254 DAJSBC010000000
VERSION     DAJSBC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.271"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..430)
                     /locus_tag="ONT65_003017"
     CDS             complement(<1..430)
                     /locus_tag="ONT65_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2330554.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAJSBC010000255          441 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJSBC010000255 DAJSBC010000000
VERSION     DAJSBC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.272"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            19..>441
                     /locus_tag="ONT65_003018"
     CDS             19..>441
                     /locus_tag="ONT65_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330555.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVL"
BASE COUNT          163 a           81 c           73 g          124 t
ORIGIN      
        1 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
       61 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      121 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      181 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      241 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      301 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      361 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      421 tccaacatga ctaaagttct g
//
LOCUS       DAJSBC010000256          436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJSBC010000256 DAJSBC010000000
VERSION     DAJSBC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.273"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>436
                     /locus_tag="ONT65_003019"
     CDS             <1..>436
                     /locus_tag="ONT65_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330556.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVD"
BASE COUNT          179 a           64 c          102 g           91 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa agtagaagtt
      421 ggaggcacag tagatc
//
LOCUS       DAJSBC010000257          436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJSBC010000257 DAJSBC010000000
VERSION     DAJSBC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.274"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            227..424
                     /locus_tag="ONT65_003020"
     CDS             227..424
                     /locus_tag="ONT65_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2330557.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           61 c           78 g          139 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taataatatt taaaccgtat
       61 atcgagtgat tatccttgtg ttagcattac atccgtgtta tagtaaataa gtaatctaat
      121 ttgaaacgta atctgagcga tatattcaca ctataaaaac tccttttacc aagtaatatt
      181 aattgcaata aaacacgtat tatatacgta tcaggaggaa atatatatga ataacggtac
      241 agtaaaatgg tttaactcag ataaaggttt tggtttcatc actggtgaag atggaaatga
      301 cgtattcgct catttctcag caatccaagg agaaggcttt aagtctttag atgaaggcca
      361 agcagttact tatgatattg aagaaggaca acgtggtcct caagcagtaa atatcgtaaa
      421 ataatgttga acgtta
//
LOCUS       DAJSBC010000258          415 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJSBC010000258 DAJSBC010000000
VERSION     DAJSBC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.275"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..339)
                     /locus_tag="ONT65_003021"
     CDS             complement(<1..339)
                     /locus_tag="ONT65_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2330558.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAJSBC010000259          409 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJSBC010000259 DAJSBC010000000
VERSION     DAJSBC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.276"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..379
                     /locus_tag="ONT65_003022"
     CDS             <1..379
                     /locus_tag="ONT65_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR2330559.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAJSBC010000260          406 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJSBC010000260 DAJSBC010000000
VERSION     DAJSBC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.278"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            92..>406
                     /locus_tag="ONT65_003023"
     CDS             92..>406
                     /locus_tag="ONT65_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2330560.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVN"
BASE COUNT          173 a           39 c           60 g          134 t
ORIGIN      
        1 tattattgtt ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga
       61 aatcttatca aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc
      121 atatagttat gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact
      181 tgggaagttt tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt
      241 acttgctgga cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca
      301 aaataaagga tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa
      361 tttaatagat tatttatcac caattgaaaa tattagatta gtaaat
//
LOCUS       DAJSBC010000261          401 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJSBC010000261 DAJSBC010000000
VERSION     DAJSBC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.279"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            143..401
                     /locus_tag="ONT65_003024"
                     /pseudo
     CDS             143..401
                     /locus_tag="ONT65_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          158 a           57 c           75 g          111 t
ORIGIN      
        1 gctattttta atggcaaaga ggggaagaag atgtttcaaa acaaaggaca acgctatgtt
       61 gtaaaaggag taaaggaatc ttactggaat caaaaatctt tattgagaaa attatctttg
      121 aatttaagca caaagaaatg aaggtacact agatagcaat gaagatatct ttgtcattaa
      181 acgtcgctat tttacacaag taagtgatga tttgattcat gtcttttcca atgagtggtt
      241 tgtttctgag aaaaaattac atgcttctaa tctgcaatat atgccaggtg aagatccaat
      301 tcctaatatg aaagcaatta tcaacagtaa ggattacgaa ggatataaag ccaaacatcc
      361 agaagcgaaa ccatttaaat acccacaaga aatgaagcga g
//
LOCUS       DAJSBC010000262          358 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJSBC010000262 DAJSBC010000000
VERSION     DAJSBC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.280"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          128 a           58 c           60 g          112 t
ORIGIN      
        1 aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt taattagtga cttatttttg
       61 attttatagc actgatacta taaaatatag atatcatcat attacacaat cttaatacta
      121 acttaaaaaa tatctccttt cacaagtatg gtgataaaat tcgttccggg ctaccttttt
      181 aggtagccta ctttaatctt tatacctttc tggatcaacg aaagtatact ttatatagtc
      241 ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc atgaatcggc tacactagac
      301 tagacagaaa aaataaggtg tgtagaataa taaaaaacac actggaggat ttttcatg
//
LOCUS       DAJSBC010000263          343 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJSBC010000263 DAJSBC010000000
VERSION     DAJSBC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.281"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          126 a           57 c           54 g          106 t
ORIGIN      
        1 attagcgatg cagggatcga gagaaacatc aaaagggtat ccttcaagat cacgttaaac
       61 aagcctaccg ataaaaagct ctcagaagcg ttctgagagc ttttgacatt ttcttgtata
      121 attttatcct aacacattac aaagcaccta attcgagctt atatcaagca aatttgctat
      181 ttattactca atacatatct aaaaattact tgatggaaaa ttagatacat ggtaagcatg
      241 aaaagaaaat caatttgagc agatgcagta caatacatag caatttaaaa gctctcagaa
      301 ggcttctgag agcttttaat atattctttg ataatttgtc ttt
//
LOCUS       DAJSBC010000264          341 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJSBC010000264 DAJSBC010000000
VERSION     DAJSBC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.282"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>341
                     /locus_tag="ONT65_003025"
     CDS             <1..>341
                     /locus_tag="ONT65_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR2330561.1"
                     /translation="RLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQ
                     NTELNEYVATLEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRL
                     SIRFGLAEKDMG"
BASE COUNT          120 a           50 c           76 g           95 t
ORIGIN      
        1 aggcttcctg atggttcgta tcatttatct gggaatgatt ttgttgattt gtatcaacga
       61 gctacgagag caacaaatgc caagagaaat gctgataaca tgagtgagca atatttacag
      121 atgcttgagc aaaatacaga attaaatgag tatgtagcta cattggaaga agagcgtcat
      181 ttgtctggta gaaaacaatt agcagattta caagtggaaa ataaaaagct gaaagaagaa
      241 aatcgctctt tacgccagcg tttagagcga ttaaaaaacg catttcaaaa atcgatcact
      301 cgtttgtcta tccgttttgg tcttgcagaa aaggatatgg g
//
LOCUS       DAJSBC010000265          321 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJSBC010000265 DAJSBC010000000
VERSION     DAJSBC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.283"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(69..321)
                     /locus_tag="ONT65_003026"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="ONT65_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAJSBC010000266          309 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJSBC010000266 DAJSBC010000000
VERSION     DAJSBC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.284"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ONT65_003027"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ONT65_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCR2330562.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           97 a           45 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatat
//
LOCUS       DAJSBC010000267          307 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJSBC010000267 DAJSBC010000000
VERSION     DAJSBC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.denovo.285"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(77..>307)
                     /locus_tag="ONT65_003028"
     CDS             complement(77..>307)
                     /locus_tag="ONT65_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330563.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           93 a           46 c           57 g          111 t
ORIGIN      
        1 gatgttggtg aagttcacca acatcaaaaa gtatagagcc gatagaagga ctgaccttct
       61 gtctcagatt tttttgttat ttcacttgaa tcaattgatt gatcaaaaaa atcctgattt
      121 tcatgttctc aaatgattta aatccataag ataatcgttt cagtgtcttg atatggggaa
      181 tcttggcctc tattttgcca ttagaatacg ggtaaatcat agcattgtaa ataccttctt
      241 catacgttaa aaggttttga agtttcgtgc gaagttgtgc atcaagtgtt tcaggaagat
      301 tttttaa
//
LOCUS       DAJSBC010000268        14795 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.063, whole genome shotgun sequence.
ACCESSION   DAJSBC010000268 DAJSBC010000000
VERSION     DAJSBC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.063"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            225..1121
                     /locus_tag="ONT65_003029"
     CDS             225..1121
                     /locus_tag="ONT65_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2330564.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1271..2212)
                     /locus_tag="ONT65_003030"
     CDS             complement(1271..2212)
                     /locus_tag="ONT65_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2330565.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2338..3459
                     /locus_tag="ONT65_003031"
     CDS             2338..3459
                     /locus_tag="ONT65_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2330566.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3717..5219
                     /gene="eat(A)"
                     /locus_tag="ONT65_003032"
     CDS             3717..5219
                     /gene="eat(A)"
                     /locus_tag="ONT65_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2330567.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5247..5813
                     /locus_tag="ONT65_003033"
     CDS             5247..5813
                     /locus_tag="ONT65_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2330568.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            6024..7085
                     /locus_tag="ONT65_003034"
     CDS             6024..7085
                     /locus_tag="ONT65_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2330569.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7088..7984
                     /gene="murQ"
                     /locus_tag="ONT65_003035"
     CDS             7088..7984
                     /gene="murQ"
                     /locus_tag="ONT65_003035"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2330570.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7998..9980
                     /locus_tag="ONT65_003036"
     CDS             7998..9980
                     /locus_tag="ONT65_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2330571.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10045..10491)
                     /gene="nrdI"
                     /locus_tag="ONT65_003037"
     CDS             complement(10045..10491)
                     /gene="nrdI"
                     /locus_tag="ONT65_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2330572.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10702..12126
                     /locus_tag="ONT65_003038"
     CDS             10702..12126
                     /locus_tag="ONT65_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2330573.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12307..13080)
                     /locus_tag="ONT65_003039"
     CDS             complement(12307..13080)
                     /locus_tag="ONT65_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2330574.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13077..13982)
                     /locus_tag="ONT65_003040"
     CDS             complement(13077..13982)
                     /locus_tag="ONT65_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2330575.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14136..14456)
                     /locus_tag="ONT65_003041"
     CDS             complement(14136..14456)
                     /locus_tag="ONT65_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2330576.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4747 a         2629 c         3114 g         4305 t
ORIGIN      
        1 gaataaatag caatatttaa caatgactaa tgataacaaa ttttcagaca attacaacta
       61 ttcagaaaac ttttaggcga attttaatag aagaatgatt tatttcatca tattttcata
      121 caaatgttaa attttcatac aaatatagga aacgttttac tggcttttta ctatttaaac
      181 aggctatcat aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg
      241 tactgctagt tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg
      301 tttggtccgg aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg
      361 caaattatca gctgatcgtt tttttagatt atggatttga tatggagatg gctcgacgcg
      421 tgcgcccatt taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac
      481 acgaggcgat gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg
      541 attttttgga agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata
      601 tgaatttttc ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga
      661 gaaaagaacg agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact
      721 atttcattct tccacatcga gggaatgaac aacctggata tcttacttat gaagagtacc
      781 tgaaaaaaac aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag
      841 tgactttgcg gacatttgaa tccatgtatt ttcaaaagaa actagtgacg gataatcaag
      901 cgattgctca ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt
      961 tagatgaatt accggaattt ctgcatacac catttaagcc aatcctatca gagctattgg
     1021 aattttatga tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg
     1081 agcaatacga atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga
     1141 gattatgtac tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt
     1201 tttgatcttt tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc
     1261 agagagcctt ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa
     1321 aggcacaata tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga
     1381 tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat
     1441 gcaacccgat gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat
     1501 ctagtatgat tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca
     1561 tgctctctgc cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata
     1621 aaccattcaa tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc
     1681 tatagatcgg atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt
     1741 cgccttctgg ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc
     1801 cgggaccagc tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca
     1861 catatttttc cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat
     1921 tgtcagtaat aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta
     1981 atgctacgat caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg
     2041 gcagttttgc gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg
     2101 cttcaatgtg agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca
     2161 tcaatccgat tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc
     2221 tctcctttat tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt
     2281 tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg
     2341 gatattttgg cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag
     2401 gcagttctaa atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat
     2461 ggcggagaaa atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt
     2521 gatgggatgg atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa
     2581 ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa
     2641 ccaacggatc aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat
     2701 gcactgcttc aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat
     2761 ggtggtctag gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat
     2821 cctttattat ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta
     2881 aaattgatta tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg
     2941 caatttggaa gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca
     3001 ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga
     3061 agtggagcag caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct
     3121 ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc
     3181 atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt
     3241 gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac
     3301 caagagctag aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca
     3361 ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga
     3421 cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta
     3481 tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc
     3541 gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt
     3601 ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca
     3661 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt
     3721 ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg
     3781 aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
     3841 gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa
     3901 tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct
     3961 attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat
     4021 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga
     4081 agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg
     4141 aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga
     4201 aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc
     4261 atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg
     4321 aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag
     4381 aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag
     4441 gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag
     4501 gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga
     4561 tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt
     4621 tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat
     4681 tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg
     4741 tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg
     4801 cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg
     4861 caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag
     4921 tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc
     4981 ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt
     5041 tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca
     5101 atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac
     5161 atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac
     5221 ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga
     5281 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     5341 gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag
     5401 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     5461 caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac
     5521 gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc
     5581 aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt
     5641 atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt
     5701 tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg
     5761 aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt
     5821 tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta
     5881 ggttatgaca attcttttga taatataaac tcaaccttta gaaatgaaat ttaattattt
     5941 taataaaagt attgaaattt tgtttcttta gatatatact ctagtcatag aaatgaagac
     6001 ggatacagaa aaagagggat aatatgtttg gcttttcaat atttatgaac gattctttgt
     6061 cagaagagaa gaaacagtat atccgccaaa tggcatccaa tggctttgtc ggtattttca
     6121 cttccatgca tataccagaa gatgatgttt cggcatatca aaagcgattg atcgaactag
     6181 gaagcgaagc gaaaaagaat aaactagaat tgatggtcga tatttcagga gaggcattgg
     6241 aacgagcggg tttttcatta gaagatctaa cgcctttgaa agcaattggt gtaacaggtt
     6301 taagaatgga ttatcatatt tccaatcagc agattgccga ctggtcacat caattgaaaa
     6361 tcagtttgaa tgctagcacg atcacgccta aagatatcga tgaattgaaa gaagcagaag
     6421 ctgatttctc acagattgaa gcttggcata attattatcc acgtcccgaa acagggctgg
     6481 acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag
     6541 gttttgtacc aggagataca gagttgagag gcccactata tcaaggattg ccaactttgg
     6601 aagaacatcg agggatccat cctcttgcag gcgctctaga tctattagca agtaacacgg
     6661 atattgttta catcggagat gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt
     6721 tccaaaaaga acagacagtc ttgctccata cggaacctgt agatgaagaa ttttacgaat
     6781 atatattagg gaagcatacg aatcggcaag acgatgcaag agatgtcata cgaagcgtag
     6841 atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag
     6901 ggtctattac tttagataac gaaaagtatt tgcgctatat gggtgagatc caattaacca
     6961 aatatgattt gccagcggat gaaaaagtga atgtagtagc taaagttatc aaagaagagc
     7021 taccattgat caaccaaatc aaagctggta tgaactatca atttataaga aaagaaggaa
     7081 gataaaaatg agcaaaatca atttagagaa cctaacaacg gaacgcagaa accaagaaac
     7141 ttttggatta gatgagatga gtatttctca agccgttcaa ctaatgaaca aagaagatca
     7201 gaaagtggcc gctgcggttg aaaaagaact gacaagtatc gaaccagtga ttgctaacac
     7261 gattgagtcc tttaaaaaag gtggacgtct gatttatatg ggagccggaa caagtggaag
     7321 actaggtgtg ttagatgctg ctgaatgcgt accgacattc ggtgtagaac cggaaatggt
     7381 cataggtcta attgctggtg gagaagaagc aatgactgta gctgtcgaag gggccgaaga
     7441 ttcttatgag ctagggcaag aagatctaat caagctgaat ttgacaaaaa atgatatagt
     7501 tattggtatc gcagctagcg gtcgtacacc atatgtgatc ggtggattag attacgcaaa
     7561 atcgatcggt gctgtcacag gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca
     7621 cgcggatttc ccaatcgaag tcgatgcagg tccagaattt ttgacaggct ctactcgatt
     7681 gaagtcagga acagcacaaa aacttatctt gaatatgatt tctactatca gtatgattgg
     7741 tataggtaaa gtattcaata acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat
     7801 cgaacgctca aaacgaatca tcatgcaagc gacagaagct gattacgaaa cagctgaaaa
     7861 gtactttgaa gaagcagatc aaaatgttaa gctggctatc gtcatgattt tgacaaatag
     7921 tgataaagag gaagctcaag caaaattagc tgaagcaaac ggatttgtaa aagggacctt
     7981 atgaggggga aaagaccatg gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa
     8041 tttataaagg ggcaggcggc atgggcaatg tagaaagcat tgtccactgc atgactcgtg
     8101 tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc tgaattaaaa gcgattccag
     8161 gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat catcggacct gggaaagtga
     8221 acaaagtagc acagcaaatg gttgatttgg caggcgtgaa attaggagaa aaactttctt
     8281 ctgtaagtac agctacagca aattcatcag catctggaaa agaccgtgta aacgaacggg
     8341 cacaagaaat gaaagcggca caaaaatcga aacaaaaacc atctaaaatc aaatcggtat
     8401 taaaagacat ttctaatatc tttgtgccaa tgattcctgc atttgtcggt actgggatcg
     8461 tggcagggat tgccgcagtt ttagcaaact tagtcacagc cggaacactt gatgcagcaa
     8521 catggcaaca atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt agctacctag
     8581 ttatctatac aggtatcaat gctgcacaag tttttggtgc aacaccaact cttggtggag
     8641 ttatcggggc cgttgtgatg ctgacaggga tgaatccgga agcaccactg aaaaacttat
     8701 ttactggtga agctcttgca gctggacaag gtgggatcct aggtgtcatc ttcgctgttt
     8761 ggctattgtc aatcgttgag aaaaaactgc ataaagtagt acctgatgcg gtagatatca
     8821 tcgttacacc aactctttca ttgattgcca tcggtctgat tgaaattttc ttcatcatgc
     8881 ctttagcagg tttcatttct gatggcatgg tcggtgggat caactgggta cttggcgttg
     8941 gtggagcatt tgccggcttc gttctaggca cattattctt gccgatggtt atgttcggac
     9001 tgcatcaaat tttgactcca atccatgtgc aaatgattga tgaaactggt cgtacgctat
     9061 tgcttccaat tcttgcgatg gctggtgcag gacaagtggg tgcagcattg gcattatgga
     9121 ttcgctgcaa aaaagataaa gaattaacag aaatgatcaa aggagcttta ccagtcggga
     9181 tcctaggaat cggcgaacca ttgatttacg gagtaacatt acctctagga cgcccattta
     9241 ttactgcttg tatcggtgga ggtatcggcg gggcagtgat cggaatgatc ggaaatgtcg
     9301 gggctatcgc tattggacct tcaggagccg ctttgatccc attgatctct gacggtaaat
     9361 ggttaggcta catccttgga ttattggcag cttatgctgg tggcttcgtt gctacattct
     9421 tcttcggtat tcctaaagac cgtttagaaa aagaagaatt agcgactgaa acagccacag
     9481 taaatgaagt gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg actatcctct
     9541 catcagttgc tgacggaaca gttgtttctt tagataaagc aagtgatcca gtttttgcac
     9601 aaaagatgat gggtgaaggc tactttgtag aaccagaaaa cggacatatc tattctccag
     9661 ttactggggt agtcagctct gtcttcccaa cgaaacacgc tattgggatc acaacgccaa
     9721 atggtttaga aatcttgctt catatgggaa tcaacacagt tgacttaggc ggtacgccat
     9781 ttgatgtgaa agtaacagaa gggcagcaag tgacacctga tacgcttgta gcagaagctg
     9841 acttagaagc gatcaaagca gcaggcaaag aaacatccat gatcgtcttg gtaaccaata
     9901 tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca aaagcacctg
     9961 tgatggatgt caaagcttaa tatgatagaa acaatagaaa cgaaagaaga aactgaaatc
    10021 tatttttcag cttcttcttt tttattattt attgttttct gctaaaatct gataaattcg
    10081 tgtcacatca gatggtgttc cccgtaattc ataatctgcc aagaaaggaa agccaaatgt
    10141 ttgtgcatat tgtttggctg ttagacaata ctgattattg aagtttttat ttccgctacc
    10201 gactacacca agacaaagtt tatgattgtc ttcatgttcc agatattcac gcatcgtttc
    10261 ggtcaagatt tctgtatcac cgttatctaa accatttccg ccgtctaaat aagtaggaac
    10321 gaaagtaaaa aatggttctg tttctttcgt aaaatcacta ttttcatgga tttcttttaa
    10381 agtaacttca ggattgacag gatcttctgc atgcattttt tcagcatatt ctgctagatg
    10441 tttggcaaag gcacgcgtat ttcctgaaat cgatatatat aaaatgttca tggatcatcc
    10501 tccaattctt tccttttagt atagcatctt ttttaagttt tttggtggtg taatttggaa
    10561 agggactagg cgctttgctt gtataatgag aaaaaagccg gtagaattat atttctatta
    10621 aagtttttat taattaagta aaaataaaaa aggtttgtta tactttttga taaaagcggt
    10681 tttattttgg aggatgctta catggataca gagcaacaga aaaaaggaat tggtttaatt
    10741 ccacttgctg ccttagtaat tggttcagcg attggaggtg gcgtatttgg tattatgacg
    10801 gacatctccg gatcggctgg cggcccagct attttcagtt gggtactcgt aggaacagca
    10861 atgttgatgc tctccttgtc gatcaataat atcaataaaa aacgtccaga tttaaaaggc
    10921 gggatcttta gttacgcaga agaaggtttt ggccgtttcg ctggttttat cagcggttgg
    10981 ggttactggc tcacatgctg gttaggcaac gtagcttttg caacattgtt gatgagcgca
    11041 ttaggatatt tcttcccagt atttgaaggt gggcaaaatc tggcatcgat cgtgatgagt
    11101 acagtctttt tatggggata tgcatggcta gtgaatcggg gagtcgaaaa tgctagtttc
    11161 gtcaatgcta tcgtcacatt ctgtaaatta gtgccattat ttttatttat tgtagtagct
    11221 atcagcacgt tttcgatcca acaattcatt gataactttt ctagcgacat tatattgaat
    11281 gcgaatggac agccgatccc gttttccagt caagtattga gttgcatcat ggtgatgctt
    11341 tgggtatttg ttggtattga aggagcctct gtcgtaggtt cacgagcaaa aaaacaatca
    11401 gatgttggga aagcaacgat tctagggata ttcggcttga ttattattta cgtcttggtt
    11461 tccatgcttc catatgggac aatgacgatg aatcagctgc aaacgatcaa ggcgcagcct
    11521 gctatgggct atgtgtttga aatgatggtt ggtaaatggg gagcagtcgt gatcaacggc
    11581 ggtttgatca tttctttagt tggtgtttgg ctctcatgga cgatcttacc gattgaaaca
    11641 atgggaaaca tggctgaaga cggacttttg cctaaagcat ggggaaaact caacaaaaat
    11701 ggggcaccaa cttatgctat cgtattgacg acgttatgta cgaatgtctt tttactttca
    11761 ttattattca ctgacagtgc ttataacttt gcttacacac ttggtacagc agctattttc
    11821 ttcacgtggc ttttcttagg attgtaccaa atgaaacttt cttatcagcg gaaagaatgg
    11881 gtccaatttt tagtagggct tttagcaagt gcctttcaag tatgggctat gctagtcgta
    11941 gcctttaatg aagtaatgct tgttcttgtt cttttcttgc caggcattta tttttacctc
    12001 aaagcacgta aggagcaaaa cgagcctgta caacatatcc acttagataa gattttactt
    12061 ataggtgtga cgatggtcgg agtgattgca atggttttat ttgccactgg gatgcttcac
    12121 gtatagaagt ataaaagaag tataaagaga ggttgtgagc ctgtcgttta ttcaaaaaca
    12181 tactaggaaa ggagctgtga cacaaatgtt gtcacagctc ctttttcaga ataaacggtg
    12241 tttaagagac agccaatcgg caataaggct gaacgtctct ttcacagcct cttttttgat
    12301 gagtcatcat acattttttc gcttaaatac gagatatcct aatgctaaga acaaaacgat
    12361 gtaagctaaa ttaccgaata gcatttgatt agtagaaaga tgagtcaatt gcttcatgat
    12421 ctcttcgttt ccaatttggt tttgcagatt caacatactg atcggattcc atttgatcca
    12481 ctcccatttt tggatgagtg caataaggat accagatatg attgacgaag caaaataaaa
    12541 gacgattcct gctgagatgg atgcgccgga actattgata aagcaagcaa tcatcaacac
    12601 taaacttaaa atcagccata aaccgatata actacccaaa gtataagtga acaagctttg
    12661 gatcagtgtt tgaccagtag aaaccttctc cgtgaaggaa atcgaaggga aaagaatcag
    12721 tttcatcaac acagtgacaa taatagctaa aaggtataaa taaacagaat aaataactaa
    12781 tgtgatccat ttgcttacta atatttctcc tcgagaatac tttcgataaa ggagattttt
    12841 caatgtacca tattgtgctt ccatcgaaat gatcgtgctt gcagctgcaa tcatgataaa
    12901 gacaatccaa gatgtggcac taaaaagctg agcagtcatt tcaactggat caatgatcgt
    12961 cgtgtatttt ttggctagaa gtgctgttcc tatgagcaaa acaactaata gaacagataa
    13021 aacagcagtt gactttttct tgatcaattt aaataattct tgttttacaa gcaatgtcat
    13081 ttttcttctt ccccctttac tgaatccaga atttccaata gagaagattc caaatcattc
    13141 gtcagatgct ggacgtctgt cacatggata ttttgtgcag acaatagctg gatcacggtt
    13201 cctaataatt cttcgttttc ttttaaaagc ttgattttat caggatcttc caaagtatat
    13261 ccagcttctg tcaagatcgt tcgagcagct agatcatttg ttgtagagat aacgatgaaa
    13321 tcttgcgctg tttggttcaa ttcagccatt gtacattgtt tgatgatctt tccttgattg
    13381 atcaggatca gctcatcgac cattttttca agctcgctga gtaagtgact ggagatcaag
    13441 aaagaggtgc cctgagtagc caagtcggta atgatttttc gcacatctct tgttgcttgt
    13501 ggatccaaac cgttcatcgg ctcatctaaa ataacgatag acggatggtt cagtaaggca
    13561 agagcaattc ccaatttttg tttcattccc aacgagtagg atttcgcttt tttgtgaatg
    13621 tacttttcca tcttgagctg attcacgata gtgtcgatag cagatacatc gttggtttca
    13681 gagaataact tcagatgatc atagcctgtt aagaatggat agatacccgg gtattcgatc
    13741 aatgctccta cttgttccaa ccctttatga ctagagggag aaatctcatt ttcaccaata
    13801 cagattgttc catctgagta atggatcagt cctaaaatag ctttcatgat cgttgttttt
    13861 ccagcgccat taggacctac taatccaacg attttgccag gggataagct aaaactgaca
    13921 tcgctgaggg cttgtttgtg accaaagttc ttggataatt cttggaccga aagacttttc
    13981 aaaaatattc actccttttc tatagatagt acaagcatac acctgatgag tggagaagtc
    14041 gctaggtcat tggacttatt tcttattttt tcataaaaaa attgaatatt aaataaaaag
    14101 gcttattttt tctgatcagt acttattcca agtgtctagt ggccgctagt aatcttgatg
    14161 ccaatgatac tgatgagtag catccccaca aagaaccaag tggaaagatt cagcttatca
    14221 tgaaacaaga ccacaccaat tatgaccgaa ccaactgcgc caatccctgt ccagatagga
    14281 taagagagac tcatcggcaa acttttgatt gcctttgaca aaaagtaaaa actagcaatc
    14341 atgccaatca ctgtgtaaaa agaataattg attttagaaa aaccatcact ccatttcatc
    14401 atcgttgacc agaatacttc caaaattcct gctatgatta attccatcca tgccatactt
    14461 acattcctcc ttaactataa aaaaatacac aaaaagagga aaatcatccc ttcaaagacc
    14521 taatggaatg atcatcctct ttatcctacc cggtggcaga ttgtatatct ttttctgtag
    14581 tatagcacac ctatccagat atcggcaatc tcatactttt tagtaaagtg taccaagcac
    14641 tgacggatat atcgttagcg gaaaagaaga tgaatgcttt gcaatttcat agaaagaagt
    14701 tgttatttat taatcattta aaaaaaacag taactagacc ttatttctat atattatcgg
    14761 gcaataaatt aaaaaaagct ttaccttaca ataag
//
LOCUS       DAJSBC010000269         9998 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.086, whole genome shotgun sequence.
ACCESSION   DAJSBC010000269 DAJSBC010000000
VERSION     DAJSBC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.086"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(158..1030)
                     /locus_tag="ONT65_003042"
     CDS             complement(158..1030)
                     /locus_tag="ONT65_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2330577.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1158..1727
                     /locus_tag="ONT65_003043"
     CDS             1158..1727
                     /locus_tag="ONT65_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR2330578.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1794..2531
                     /locus_tag="ONT65_003044"
     CDS             1794..2531
                     /locus_tag="ONT65_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2330579.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2762..4030
                     /locus_tag="ONT65_003045"
     CDS             2762..4030
                     /locus_tag="ONT65_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2330580.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4517..4981
                     /locus_tag="ONT65_003046"
     CDS             4517..4981
                     /locus_tag="ONT65_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2330581.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4965..5594
                     /locus_tag="ONT65_003047"
     CDS             4965..5594
                     /locus_tag="ONT65_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2330582.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5948..7426
                     /gene="msr(C)"
                     /locus_tag="ONT65_003048"
     CDS             5948..7426
                     /gene="msr(C)"
                     /locus_tag="ONT65_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2330583.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7473..7919
                     /locus_tag="ONT65_003049"
     CDS             7473..7919
                     /locus_tag="ONT65_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2330584.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8118..8633)
                     /locus_tag="ONT65_003050"
     CDS             complement(8118..8633)
                     /locus_tag="ONT65_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2330585.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8750..9952
                     /locus_tag="ONT65_003051"
     CDS             8750..9952
                     /locus_tag="ONT65_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2330586.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3189 a         1750 c         2075 g         2984 t
ORIGIN      
        1 gttttgtcaa ttcttttttt ataattatag taatttactg gaattatcag aaataaaagg
       61 aaggaacaaa ttaagaaact tatgttattt aatcattgga tcttttgctt aacttattcg
      121 ttgttaaaaa gctgaaagcg aaggaaaaaa actctactta actgtatttc tccgttagga
      181 catctaaaaa tttacgagca gcaggagaaa gaggctggtc ttttttccaa atgagattca
      241 gtgaagcttg taatggcgga tcgagtggga tgaatacttt ttccgtccca taagtattga
      301 tgatcccatc tagagctaat gcaccaaaag tcccgttatc gaccatcaaa gaagcattgt
      361 acaataagtt atatgttccg acgatcgata actgatctat gctgaaacct agccattcag
      421 ctagttgact gtccactgac gtttgccggg aaatgatcaa aggaacagtt ttcagttcct
      481 gcggatggat gactgtttgc tttgctaaag gatgattccg atgtatcaaa agtccccaac
      541 gatcttgggc tggcagtcga aatgactggt atttttgttt gtttgtcggt tcgattacga
      601 gtccaaaatc aagtaatccg ctatccagtt tgtctatgat atcatctgca ttcccgctat
      661 ataaatgaaa ctggatatca ggatgcgcct tgatcaacga atgaaggcta tcagaaatga
      721 aagagattgc tttagtttcg cctccgccaa tgtagatttc tccagagatg attgcgtcat
      781 ttgttaaatt gttcgccgtt ttttctacca acgacaagat ttcttttcct tttgaataaa
      841 gatatcgacc agaatccgtc aactgtatct ctcgggagcc tcgagtgaaa agtgtcacac
      901 caagttcttc ttctaaatct ttcagttgtt ttgagagagt aggttgcgtg atatgcagag
      961 attttgctgc tcggctgata tttttttctc tagctactgc taaaaaatac gtcagtatac
     1021 gaatatccat tttttccctc ctgtttttaa aaaagcatac cactaagtat agttaaaagg
     1081 aatagttaag tatgttttat aagtattaga catcataagc cgtccgttat aaagtaagga
     1141 taaaaggagg gagcaaaatg acaagcctca tcgaaaaaca aatcgtcaat aaagagattc
     1201 gagtaacgga tccgctcttt gaagtgatcc atcagatcca agcggaaaac gaagagcctt
     1261 tagcagcgct gaatactggg taccatgaac cagcagatat ccgcaaacgg ctagaaaata
     1321 tcattagtga caaagtagat gatacggtga ctgttctgct gcctttttat acagatttcg
     1381 gcaaacatat tagtataggg aaaaatgtct ttatcaatcg tcaagtgatg ttcgttgact
     1441 taggtggcat ctgtttagaa gattcagtat tgattggtcc tcgtgtcaac ctcatcacgg
     1501 tgaaccatct aaccgatcca aaagaacgaa gaggcttgtc agtaaagcca atccacatca
     1561 agaaaaatgc ttggatcgga gcaggagcaa ctattctgcc aggcgtaacg attggcgaga
     1621 atgccattgt tgcagcgaat gcaactgtta cgaaagatgt cccagacaat accatcgttg
     1681 caggaatccc tgccaaaatc gtcaaacctg tcgaacggat tatttgaaca gtaagatgaa
     1741 ataagagata gtgagtataa gcaagagatt tttcaaaaca ggaggagaat agaatgaatg
     1801 atttatcaga aaaagtgatt gtcatcatgg gcgcttcaag cggaatcggt gaagcaacaa
     1861 cgaagctatt agcagaaaaa ggggctaaac tagttatagc tgcacgccga gaagatcgcc
     1921 taaaagcaat aaaagaatct ttaccagaag ctgagctata tattcaaaca gcagatgtaa
     1981 gagattttgc acaagtccaa gcagttatcg atttagcgat ggaaaagttc ggtcgaatcg
     2041 atgtattgta caataatgca ggaatcatgc caaccgctcc attagttgaa ggtcaccgtg
     2101 atgaatggca aaatatgctt gatatcaaca tcatgggtgt gttaaatgga atttccgccg
     2161 tacttccaat catggaaaaa caaaaatctg ggcatatcat ttcaactgat tctgtggcgg
     2221 gacatgttgt ttatcctgat tccgccgttt attgcggaac aaaatttgct gtccgtgcaa
     2281 tcatggaagg attacgtcaa gagcaacggc aaaataacat caaatctacg atcatctctc
     2341 caggagctgt tcaaactgaa ttatatcaaa ccatcagcga caaaaaggct gctcttgaac
     2401 ttcatgaagc gcaaaaagaa tggggattaa catctgaaga tatagcttct gctgtagcat
     2461 ttgcgataga aacgccagac cggatgtctg tcagcaacat gattatccgt ccgacgacac
     2521 aggaagtata atccataacg gataatatcc agagagaatt acttgttaaa tagttattca
     2581 aacgaccatc aatttaaggc gtgaatggag aagaatattc ttcatttacg tctttttatg
     2641 cagaaaaagc aaatttatta aaaagaaaca aatgttattt aaatatggac attgacttgc
     2701 ttttactttt ttagttacac tgttatttag gaaaagtttc ataattcaaa ggagaaaaaa
     2761 gatgaaagca aagaatttat tatttagcgt tattctttta ggaaatctag cagttgccaa
     2821 cacaacagtg ctagctgaag aatcgacaga aactgtcgca actcagacat cgagttcttc
     2881 agaaacagga attgaaaacg atacagaaag tactttgcag tcagctactg tctcagaaaa
     2941 tacaatttca accgaagaac caacaactga aacaacagaa caaacacaaa ccgaagacag
     3001 tacatcagca gaagctcaga acaatccaga gaatgcagaa gaaatcacga accaattgac
     3061 agatgggcaa acattgacac atacccatca agcagatttg tctgaattcg aaaactatac
     3121 tggctatgct ttttatgctc gctcatctgc taattctcaa caagcgttca ttgatagtat
     3181 tgcctcaact gcacaaagtt tggcaagtgc caatgactta tatgcatcag tcatgattgc
     3241 tcaagccatc gttgaaagtg gctgggggaa cagtactttg gcttctgccc ctaattacaa
     3301 tctatttggg attaaaggaa gctataatgg ccaatctgtt acgatgccta cttcggaata
     3361 tgtgaatggg caatggatta cggtgaatgc cgcttttaga aaatatcctt cgtacaaaga
     3421 atcattgcaa gataacgtaa cggtattgaa gacgacttct tttcagccag gcgtttacta
     3481 ctattccggt gcatggaaaa gcaatacgaa ctcttacaaa gatgctacgg cttggctaac
     3541 aggtcgttat gccactgcgc ctaattatgg ttctacattg aacaatgtaa tcgaaaccta
     3601 taatctgaca cagtatgata cagcaccaac ttcaacttct ccgatgtatc gtctgtataa
     3661 ccgtcataca ggagaacatt tatatacatt gaatgccgga gaaaaaaatt atctgccaac
     3721 tgtaggctgg gaatatgaag ggattgcttg gcaagcacca aacagcggac aacctgtcta
     3781 ccgtttatac aatccttaca gtggagacca tcattacaca atggcacaat ctgagatcaa
     3841 ctctttaaca aaaatcggtt ggcgttatga aggtctaagt ttttattctg gtggttccaa
     3901 gccaatttat cgactattta atccaaatga aaaaactggt acccatcact ataccttgag
     3961 tgctaaggaa agagattggc taacaccgat gggctggaga tatgaaggaa taggatttta
     4021 tggttattaa aaagaggtag tgaaaaagct gccctgcatc aagtagtaag aacagccaaa
     4081 caaaaacaga ttttcatgtt ttttgtttgg ttgaacttat taccgcagat gcaagttttt
     4141 gaacaccgtt tactcaaaaa aggttgtgac aacatcttgt cataactttt ttattactgt
     4201 ttaagcatac tgatccaatt tctaatattc gacgggattc ttgcaaaagg cgatttcgga
     4261 aatttttggc ggtgtaaaat tatctactgc agaagagcta tcttctatag tttgataaga
     4321 atcaataatc ttggttttag cgttgtgctt tctttatcat tttttccgtt cattctacaa
     4381 aaaatttctt ttctctatac ttttctattg agagcgtttt ttttattaca cagctgattc
     4441 tttgactttt ttcttttttt attctattat tgacaagtca atcgttgacg agtcaattat
     4501 tagaaaggag gaaattatgt ctttagaaga gattagtcac cttttatatc aaatcaaatt
     4561 agccgatcaa aaaatcgtcc agatctttga agaaaatatc ggtataagtc ttactagata
     4621 cgagatcatg atgagactga aagaagaaaa tccattgcta caaagcagtt tgcaagaaaa
     4681 gttgcagatt gatcaaggag cagttactcg tcatctgaag gtattggaag caaatggcta
     4741 tgtttcgcgt cagagaaatc ctcagaacaa ccgggaagtg tatgtagcgc taacagaaaa
     4801 agcccagaag aaaatcaagc attgtgaaat ggaccataaa gatacaggtt ccgtgctatc
     4861 cccggctttc agcgatgtag aaatgaaaca atttctaaca cttttagata aattgaatac
     4921 ccaactttgc aatataacaa cagaaacgaa agaggttaat aataatggaa caaaaaaatg
     4981 atttttcaac aattatgact ggtagaagat ccgttcgcgt atatgatgaa acatacaaaa
     5041 tcccacacga agaaatgctg gaaatgatca aagaagcgac gactgcacct tcttctgtca
     5101 atatgaaacc atggcgcttt gtggtcgctg aaagcgagga agcaaaagaa atcttgcgtc
     5161 ctttgatccg tttcaatacg agacaaaatg atacttcttc tgcaatggtc atgatttttg
     5221 gcgatatgca atgttacgaa tatggcgaag aaatctacaa ccaagcttac gaatcaggga
     5281 agatgccaaa agaagtcaga gatcaacaat tagctgcgat cattccttat tacaagagtt
     5341 tttcaagaga agaaatgaac gatgtagtca aagtcgattc tagcttagcg gctatgcaat
     5401 tcatgttagt tgcgcgtgat catggctatg aaacaaatcc aattggtgga ttcgaagcag
     5461 atcagttggc agaggcattt ggtttggata aagaccgtta tgttccagtt attattttat
     5521 caataggaaa agcggtagaa gaaggttacg agtctgttcg tttagcacca gaaaagatca
     5581 ctatgttcga ataaaaggtt ttcagttact attcagtagc tattgctttt tttgaatcgt
     5641 gtgtggtaac ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga aattacgttt
     5701 cgaacttttg aataacaact aaataaattc aaaagacatg tataaacaac aatttcatgg
     5761 atggagtatg tccatttttt cgaacgacag tagaataggt gaaagctatg acagatttgg
     5821 tgatttagtc atagttattt cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa
     5881 acagattttt ctgctctttt ttagtatcga taattgttgt tttacatgaa ataggagtgt
     5941 aaaacatatg gaaaatttag cagtaaatat aacaaatcta caagtcagct ttggcaacca
     6001 gctagaacta tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg
     6061 agaaaatggt gtcggtaaat cgactttgct caaactaata gccggtgaac tttttcccga
     6121 tcatgggaaa atccaaacag agatcacctt caactacctg cctcaattaa cctatctcgc
     6181 tgaagcaaag gacctaaatt tggaattagc cagtcatttc cagttaagac tggaagaaac
     6241 ttcagagcgg aaatggagcg gaggggaaga acgaaagatc gagttgatac gtcttctttc
     6301 ttcttatgaa caagggatgc ttctagacga gccaacaacc catctagata gaaaaagtat
     6361 tgatcgactg attgaagagc ttcgttatta ttatggtacg ctggtttttg ttagtcatga
     6421 tcgctatttt ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg
     6481 agagttttcg gggaattata gtgcctatct cactcaaaag gaattggaga aaaagactca
     6541 gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga
     6601 aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat
     6661 cagactggat cggttgtctt cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa
     6721 gaatgcgaaa acattagaga gaagactcca aaaaatagga gaaacaacca agccgcagca
     6781 gatgaaacaa atccgttttc cagtaccaaa atctcttgaa ctccacagcc gctatcccat
     6841 catgggacaa aatgtccaat tggaaagaag tgggagaaca ttactggtaa atggtgattt
     6901 tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac
     6961 cttattagaa catatccgca aacaaggaga aggaatcctt ctctctccga aagtaagctt
     7021 tcaagtatat cagcaaaagg gttatcaaat gacatctgaa gaatccatca ttcgttttgt
     7081 catgagacaa acagagtttt cagaatcgct tgtccgtagt ttgctgaatc acttagggtt
     7141 tgctcaggaa actttgacga aaccgttatg tacattaagt gggggagaag cgacccgtct
     7201 gacgattgct ttgcttttta ctaagccaag taatgtgttg ctgttagatg aaccgactaa
     7261 ttttattgat atggcaacga tcgaagcttt agagaagcta atgcaaatat atccgggaac
     7321 gattttgttt acttctcatg attcttactt tgtcgagcgt acggctgatg aagtttatga
     7381 aataaaaggg cagaaaataa aaaaagtact tacgagaaat ttttaaatgt gctacgatga
     7441 ttttcgtgac aaatttaaag gagtggtaca gattgcctac aaataaaaaa gaaggaataa
     7501 tttttacaac aatgatgtgt tttttgatgg tcttggggat gagcgcgtat aatttatggc
     7561 tgcatcaaga tcttcattta tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg
     7621 ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc tttaaactac
     7681 cagtaaatcc agaaagcaag gtccaaatga tcttaactat ttcatgcttg atggttttag
     7741 gaatggtgac atgtatgtct ttattcggtg tgatcatgga acaaggaata cctgatcaat
     7801 tattctctgc ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt
     7861 tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat
     7921 ggaacactgt tatttgaaaa aaggccttgg catgcttttt gtcatagtct cttgaatgca
     7981 gttctagaaa aatagtgatc cgaacgatag gttttggcct gtcgttcgga tcgctttttc
     8041 ttttccaact gtattgctct tttattgaag ataatttgct cttcaataga tatttctaga
     8101 aaggttctgt ttatccctta ctcaaaaaac tgctgcattt gttctcttac ttgctgacac
     8161 cattggagtt cctgctgaca aagcataatt ctgcgctgga tcacaacata acgtccaaat
     8221 tcttgtttct tgtctagcga gaggtcattc aatttttttt gatttttttg catgattttg
     8281 ttcagctgtt gttctctagt gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa
     8341 aaagcataga tttttgctag aaattcatct ttttgcgtag gagcgggagt ttcctctttg
     8401 atccattcct ctacaagagc acgtccttca tcagtcaagt ggtagatctt tttttgagag
     8461 tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt ctaacttggc aagcgttgta
     8521 tagatctgac tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa ttcataccca
     8581 gaacgtggtt tggataaaag cacgcataat aatatatagg ctaaagtatt cataaaaatc
     8641 ctacctctcg tcttattttc tttattatat cacagtaaag tttccacgtt ttacatgtgc
     8701 atattgacat gtaaaataaa tcatgtatga tgctaataga gagaaaggga tggaaatatt
     8761 ggagaatcaa atagatatac aagaacaaac aaatgaagaa acaaagggga aaagttcagc
     8821 gctagttgta gcagctacct gtatgatcgc ttttatggga gtgggtctag tagacccaat
     8881 cttaaaaacg attgctgcac agttaaacgc tacgccagct gaaacgaccc tgttatttac
     8941 tagttacatg ttagtcactg gtgtggtgat gctatttaca ggtttcttat ctagtcggat
     9001 cggtttgaaa aaaacattga tgacaggatt gtttatcatc gttgtattcg ctggtcttgg
     9061 cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg gtgtcgggaa
     9121 tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg gtaacacaga
     9181 aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg gccctctagt
     9241 cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag catttttgat
     9301 gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac caaagagaaa
     9361 agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt cagttggact
     9421 aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc catttttatt
     9481 gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg tgcttctggc
     9541 tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt atcggacaat
     9601 tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg gaacttctat
     9661 gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa ttatcaatac
     9721 actgttgacg acgattgcta tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag
     9781 ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag gtgtgattgg
     9841 tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc ttatcagtgt
     9901 atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat aagaaatcag
     9961 aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DAJSBC010000270         5873 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.117, whole genome shotgun sequence.
ACCESSION   DAJSBC010000270 DAJSBC010000000
VERSION     DAJSBC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.117"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(41..1027)
                     /locus_tag="ONT65_003052"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ONT65_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="ONT65_003053"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="ONT65_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2330587.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ONT65_003054"
     CDS             1852..3006
                     /locus_tag="ONT65_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2330588.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ONT65_003055"
     CDS             3221..4189
                     /locus_tag="ONT65_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2330589.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ONT65_003056"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ONT65_003056"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2330590.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="ONT65_003057"
     CDS             5219..5827
                     /locus_tag="ONT65_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2330591.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1772 a         1144 c         1320 g         1637 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaacggtt ctgttgcaaa gttttaaatc tactatcaaa taa
//
LOCUS       DAJSBC010000271         3558 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.147, whole genome shotgun sequence.
ACCESSION   DAJSBC010000271 DAJSBC010000000
VERSION     DAJSBC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.147"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1170
                     /locus_tag="ONT65_003058"
     CDS             <1..1170
                     /locus_tag="ONT65_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2330592.1"
                     /translation="AQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPS
                     TITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHL
                     DKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPIS
                     KEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEI
                     YRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQE
                     KLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDC
                     THFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1177..1554
                     /locus_tag="ONT65_003059"
     CDS             1177..1554
                     /locus_tag="ONT65_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2330593.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1708..2490
                     /locus_tag="ONT65_003060"
     CDS             1708..2490
                     /locus_tag="ONT65_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2330594.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2616..3347)
                     /gene="erm(A)"
                     /locus_tag="ONT65_003061"
     CDS             complement(2616..3347)
                     /gene="erm(A)"
                     /locus_tag="ONT65_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2330595.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3405..3464)
                     /locus_tag="ONT65_003062"
                     /pseudo
     CDS             complement(3405..3464)
                     /locus_tag="ONT65_003062"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
BASE COUNT         1258 a          529 c          669 g         1102 t
ORIGIN      
        1 gcccaaagac ttaaagagtt ctttaatttt ctggacatga aatttaaaca agttcagaga
       61 gtacaccaat taacgagagt ggaaattgaa gcatatttaa gtgaactaaa catgatggga
      121 ataaaaccta gtacaataac tgggaggatc tctatattgg aaggactatt tagtaccctt
      181 cttaggctag aatgggatga tgttccttcc aaaatattaa tttattctga ggactatccg
      241 aaaataccaa gagcaaaacc acgctttata gatgaattcg tcctagagca attgaacagt
      301 catcttgata aattacccga atatatagct acgatgacta tgattgttca agaatgtgga
      361 atgaggataa gtgaattgtg caccttgaaa aaaggctgtc tattagagga caaagatgga
      421 gatttctttt taaagtatta tcaatggaaa atgaaaaagg agcatatagt tccaatatct
      481 aaagaggtag ctttacttat taaagttcgg gaagataaag tttcagagga atttccagat
      541 agtgaatacc tctttccaag aaaagatgga tcgccattaa aacaagaaac atttagaggt
      601 gagttaaata aattagctta tgagcaaaat atagtggata aatcaggtga gatttataga
      661 ttccatgccc atgcctttcg ccatacagta ggaacaagaa tgattaacaa cgggatgccc
      721 cagcatattg tgcagaaatt tttggggcat gaaagcccag aaatgacaag cagatacgct
      781 catatctttg atgaaactct aaaaaatgaa tttactaaat ttcaggaaaa actggttacc
      841 aataatggag atgtgcttga tctagatgaa gataatgaag tcgatgatgt agagcttcaa
      901 tggttcaaga aaaatataaa tgcacaagtg cttccaaatg gttattgtag attgccagta
      961 gtagcaggtg gttgtccaca tgcgaatgca tgcttagatt gcactcactt ctgtaccagt
     1021 aagcaattct taccacagca cgaagaacag ttagagcgta cagaagagtt attagccata
     1081 gctaaggata aacaatggca aagacaagta gagactaata gccgtgttaa agagcgttta
     1141 gaacaaatca ttggaagttt gacggggtaa ttatcaatgg ataaacaagt tagaaataca
     1201 acagaaattg tacgtttggc gaagcagaaa tcaaaaaaga caagggaaaa agtagacaaa
     1261 gcgatttcta aattttcgat tgaaggtaaa gttattaatt ttaattcaat agcaaaggaa
     1321 gctaatgttt ctaaatcatg gctttataag gaacacgata ttaggcaaag aatcgaatcc
     1381 cttcgtgagc gtcaaataac agcaaatgta gtctcaaaac ccaagaaaag ttctcgttcg
     1441 gaggaaatcc ttattaaaac cttaaaaaga agagtaatgg aattagaaaa agaaaataaa
     1501 aaattacaga accaaattca aaaattatat ggagatctgt ataataaaga ataattatta
     1561 atctgtagac aaattgtgaa aggatgtact taaacgctaa cggtcagctt tattgaacag
     1621 tcatttaggt atatgtccaa tctagggtaa gtaaattgag tatcaatata aactttatat
     1681 gaacataatc agttacgagg tgaaatcatg agcaatttga ttaacggaaa aataccaaat
     1741 caagcgattc aaacattaaa aatcgtaaaa gatttatttg gaagttcaat agttggagta
     1801 tatctatttg gttcagcagt aaatggtggt ttacgcatta acagcgatgt agatgttcta
     1861 gtcgtcgtga atcatagttt acctcaatta actcgaaaaa aactaacaga aagactaatg
     1921 actatatcag gaaagattgg aaatacggat tctgttagac cacttgaagt tacggttata
     1981 aataggagtg aagttgtccc ttggcaatat cctccaaaaa gagaatttat atacggtgag
     2041 tggctcaggg gtgaatttga gaatggacaa attcaggaac caagctatga tcctgatttg
     2101 gctattgttt tagcacaagc aagaaagaat agtatttctc tatttggtcc tgattcttca
     2161 agtatacttg tctccgtacc tttgacagat attcgaagag caattaagga ttctttgcca
     2221 gaactaattg aggggataaa aggtgatgag cgtaatgtaa ttttaaccct agctcgaatg
     2281 tggcaaacag tgactactgg tgaaattacc tcgaaagatg tcgctgcaga atgggctata
     2341 cctcttttac ctaaagagca tgtaacttta ctggatatag ctagaaaagg ctatcgggga
     2401 gagtgtgatg ataagtggga aggactatat tcaaaggtga aagcactcgt taagtatatg
     2461 aaaaattcta tagaaacttc tctcaattag gctaatttta ttgcaataac aggtgcttac
     2521 ttttaaaact actgatttat tgataaatat tgaacaattt ttgggaagaa taaagcgtcc
     2581 tcttgtgaaa ttagagaacg ctttattact ttaatttagt gaaacaattt gtaactattg
     2641 aaaatagaaa gaaattgttc cttcgatagt ttattaatat tagtgacatt tgcatgcttc
     2701 aaagcctgtc ggaattggtt tttagtgaaa agaacacgat attcacggtt tacccactta
     2761 taaacaaaag atcgatactt tttgtagtcc ttctttgaaa tcaatggttg atgtcgttca
     2821 agaacaatca atacagagtc tacacttggc ttaggatgaa aatatagtgg tggtactttt
     2881 ttgagcattt ttatatccat ctccaccatt aatagtaaac ccaaagctcg ttgcagattt
     2941 tgcaatcttt tcgcaaatcc cttctcaacg ataagatagc tatatttagc ctgactttca
     3001 aaggtaattc ttttgacaat atccgtactg atgttataag gaatattacc atatatctta
     3061 tagtttatat gttttgggaa ggaaaatttt agaatatccg tttgaatcac ttttatattc
     3121 tcagaggggt ttaccgcttc tttagtcact tgacataagc ctccatcaat ttctatagca
     3181 gtaactgatc gactcatttt gactagctct ttggtaaaat gtccttttcc tgatccgatt
     3241 tctattacgt tgtcttgttt actgatattc gtgtgattca atatttcttt tacatgcttt
     3301 ttagaagtaa taaaattttg cgtgtcttta gggtttttct ggttcattat aaccttctcc
     3361 ttactggtta taatgaactg acattaagcg ttcattataa ccaattaatt ttgatttggt
     3421 tgataacgaa ctttattaat aacaaatata gaaaaagtac ccatactttt atctcctttc
     3481 catttttttc attatgaatg agataaagta atttctacta ctgcgatact ggtgcacata
     3541 attgaaacct ccttatgt
//
LOCUS       DAJSBC010000272         3514 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.148, whole genome shotgun sequence.
ACCESSION   DAJSBC010000272 DAJSBC010000000
VERSION     DAJSBC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.148"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            240..737
                     /gene="dfrG"
                     /locus_tag="ONT65_003063"
     CDS             240..737
                     /gene="dfrG"
                     /locus_tag="ONT65_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2330596.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(809..2761)
                     /locus_tag="ONT65_003064"
     CDS             complement(809..2761)
                     /locus_tag="ONT65_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2330597.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2768..3004)
                     /locus_tag="ONT65_003065"
     CDS             complement(2768..3004)
                     /locus_tag="ONT65_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2330598.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3239..>3514)
                     /locus_tag="ONT65_003066"
     CDS             complement(3239..>3514)
                     /locus_tag="ONT65_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2330599.1"
                     /translation="TVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGS
                     AKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1080 a          613 c          571 g         1250 t
ORIGIN      
        1 tgtacttcaa aatggactac ttttttcatt ttaacttaca taccaaatta catacaaaat
       61 gatttataaa atgcaattga aaatgataat tgaatgaaaa tttgtgattt tgtggtatta
      121 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
      181 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
      241 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
      301 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
      361 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
      421 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
      481 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
      541 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
      601 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
      661 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
      721 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
      781 agcttgggat tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa
      841 attgtttatc tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt
      901 taaaagggcg acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact
      961 tcggattgaa gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc
     1021 tttcaagaga tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat
     1081 taacatttaa tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac
     1141 aatcaactga ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg
     1201 catatttgcg atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg
     1261 ttagtaattc agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc
     1321 tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt
     1381 ttctgctttt cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt
     1441 ctttcgtgaa tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc
     1501 gccattcgtc ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag
     1561 gagcatcaac atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt
     1621 gaagataagc attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc
     1681 aatctaacct atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg
     1741 tatcttcatt taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca
     1801 catatcttga taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa
     1861 accccgtata tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca
     1921 tcttgtccgt attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat
     1981 atcgttctaa aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc
     2041 caactccaat tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct
     2101 tagcgaacat tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt
     2161 ttggtagaac attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc
     2221 tacttttccc ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg
     2281 agcagccttc tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta
     2341 taaggcgttt aatttcttca acaacagacc aattagataa aggggttact gtacagtttt
     2401 gcgatacacc tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt
     2461 tggaactacc agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct
     2521 gttctttccc aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt
     2581 cagaaccgtt ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa
     2641 atcgttttgg agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa
     2701 tcggaatttt aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca
     2761 ttgtctatca acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc
     2821 gcttgttggc aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat
     2881 ccactgcttc gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta
     2941 cttgctctga tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt
     3001 gcataggaaa atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt
     3061 gcattcctac aataattatt tcacgttcgc atttttatat acattttaac ttacattttg
     3121 tggtgtaaat taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg
     3181 caatttgata ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt
     3241 aatatgcaag acgttgtctt ttttttgtat attcaatttt gaaaacattt ccgcaaagtt
     3301 cagacggacc tcgatgtgtt gatgaataaa atttattagt aataacttct ataaggttat
     3361 caatccctat attacttttt gcacttccat gataaagagg gaacagagaa caattctgaa
     3421 atcttatgct ttcctcttgt tcgagttcca atgcttctaa tgatttaccg gacatatatt
     3481 tctctaaaag gtcatcgttt ccctctatta ccgt
//
LOCUS       DAJSBC010000273         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.178, whole genome shotgun sequence.
ACCESSION   DAJSBC010000273 DAJSBC010000000
VERSION     DAJSBC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.178"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003067"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2330600.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT65_003068"
     CDS             2060..2227
                     /locus_tag="ONT65_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2330601.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT65_003069"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT65_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2330602.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          817 a          361 c          483 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSBC010000274         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.179, whole genome shotgun sequence.
ACCESSION   DAJSBC010000274 DAJSBC010000000
VERSION     DAJSBC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.179"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003070"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2330603.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT65_003071"
     CDS             2060..2227
                     /locus_tag="ONT65_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2330604.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT65_003072"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT65_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2330605.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          816 a          361 c          484 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSBC010000275         2369 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.180, whole genome shotgun sequence.
ACCESSION   DAJSBC010000275 DAJSBC010000000
VERSION     DAJSBC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.180"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003073"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2330606.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ONT65_003074"
     CDS             2039..2227
                     /locus_tag="ONT65_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2330607.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="ONT65_003075"
     CDS             complement(2285..>2369)
                     /locus_tag="ONT65_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2330608.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          814 a          363 c          469 g          723 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAJSBC010000276         2369 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.181, whole genome shotgun sequence.
ACCESSION   DAJSBC010000276 DAJSBC010000000
VERSION     DAJSBC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.181"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003076"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT65_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2330609.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ONT65_003077"
     CDS             2039..2227
                     /locus_tag="ONT65_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2330610.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="ONT65_003078"
     CDS             complement(2285..>2369)
                     /locus_tag="ONT65_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2330611.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          813 a          363 c          470 g          723 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAJSBC010000277         1781 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.195, whole genome shotgun sequence.
ACCESSION   DAJSBC010000277 DAJSBC010000000
VERSION     DAJSBC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.195"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            24..416
                     /locus_tag="ONT65_003079"
     CDS             24..416
                     /locus_tag="ONT65_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2330612.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ONT65_003080"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ONT65_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR2330613.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAJSBC010000278         1590 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.201, whole genome shotgun sequence.
ACCESSION   DAJSBC010000278 DAJSBC010000000
VERSION     DAJSBC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.201"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            122..1162
                     /locus_tag="ONT65_003081"
     CDS             122..1162
                     /locus_tag="ONT65_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2330614.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1260..1448
                     /locus_tag="ONT65_003082"
     CDS             1260..1448
                     /locus_tag="ONT65_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2330615.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1506..>1590)
                     /locus_tag="ONT65_003083"
     CDS             complement(1506..>1590)
                     /locus_tag="ONT65_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2330616.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          531 a          248 c          300 g          511 t
ORIGIN      
        1 tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg
       61 agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga
      121 aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat
      181 tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt
      241 gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag
      301 taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc
      361 gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa
      421 agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact
      481 aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca
      541 catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc
      601 gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt
      661 tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa
      721 tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc
      781 agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa aagctggaac
      841 agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat atctttcacg
      901 ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac tgaaaaataa
      961 tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc gcaatgattt
     1021 aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat atcaggttac
     1081 cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata aagtaagata
     1141 tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg tttcttatta
     1201 aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag gagattgtta
     1261 ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac
     1321 gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag aacgaaactt
     1381 taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc aagacgcaga
     1441 gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta tctgaatttt
     1501 actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc ttccattaag
     1561 gatagttctt tgtctgtaaa gctatccatg
//
LOCUS       DAJSBC010000279         1382 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.215, whole genome shotgun sequence.
ACCESSION   DAJSBC010000279 DAJSBC010000000
VERSION     DAJSBC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.215"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1311
                     /locus_tag="ONT65_003084"
     CDS             <1..1311
                     /locus_tag="ONT65_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HCR2330617.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAJSBC010000280         1350 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.218, whole genome shotgun sequence.
ACCESSION   DAJSBC010000280 DAJSBC010000000
VERSION     DAJSBC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.218"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            47..298
                     /locus_tag="ONT65_003085"
                     /pseudo
     CDS             47..298
                     /locus_tag="ONT65_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ONT65_003086"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ONT65_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2330618.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAJSBC010000281         1083 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.231, whole genome shotgun sequence.
ACCESSION   DAJSBC010000281 DAJSBC010000000
VERSION     DAJSBC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.231"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            368..916
                     /gene="aac(6')"
                     /locus_tag="ONT65_003087"
     CDS             368..916
                     /gene="aac(6')"
                     /locus_tag="ONT65_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2330619.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          377 a          157 c          215 g          334 t
ORIGIN      
        1 aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta
       61 tctaaaaatt ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata
      121 attggttttt tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc
      181 tccaattgat ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt
      241 ttattttata agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt
      301 tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg
      361 aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc
      421 tgatttactg agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga
      481 agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg
      541 atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt
      601 agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga
      661 agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac
      721 aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa
      781 ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt
      841 accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg
      901 accaaattct caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga
      961 aggaatttac gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag
     1021 ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa
     1081 gaa
//
LOCUS       DAJSBC010000282          910 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED16-08
            SAMN30692180-rid15768313.guided.242, whole genome shotgun sequence.
ACCESSION   DAJSBC010000282 DAJSBC010000000
VERSION     DAJSBC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692180
            Sequence Read Archive: SRR21444893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472552.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472552.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 22 of 69
            Pseudo Genes (incomplete)         :: 37 of 69
            Pseudo Genes (internal stop)      :: 17 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692180-rid15768313.guided.242"
                     /isolate="VRED16-08"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            8..745
                     /gene="erm(B)"
                     /locus_tag="ONT65_003088"
     CDS             8..745
                     /gene="erm(B)"
                     /locus_tag="ONT65_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2330620.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          347 a          155 c          138 g          270 t
ORIGIN      
        1 tgataacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact
       61 caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac
      121 aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga
      181 attagacagt catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac
      241 tttaattcac caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt
      301 tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagccg
      361 tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca
      421 ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc
      481 agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca
      541 taccacagat gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt
      601 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc
      661 caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta
      721 tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac
      781 acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa taactaccaa
      841 ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc tatttcttat
      901 cgatacaaat
//
